AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280009395.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009395.01.T01 MTR_2g061790 91.444 187 11 2 1 186 48 230 9.96e-96 277
MsG0280009395.01.T01 MTR_1g095030 52.941 119 39 4 68 184 19 122 1.28e-26 98.6
MsG0280009395.01.T01 MTR_1g114320 34.437 151 92 2 28 178 166 309 1.25e-20 87.4
MsG0280009395.01.T01 MTR_7g074170 36.000 100 59 1 29 128 318 412 9.45e-20 86.3
MsG0280009395.01.T01 MTR_4g110100 46.429 84 40 1 29 112 333 411 1.19e-19 85.5
MsG0280009395.01.T01 MTR_2g097620 40.860 93 46 2 29 121 163 246 1.52e-18 82.0
MsG0280009395.01.T01 MTR_6g079660 43.529 85 43 1 28 112 207 286 1.82e-18 82.0
MsG0280009395.01.T01 MTR_1g114620 44.318 88 41 1 28 115 239 318 2.03e-18 81.6
MsG0280009395.01.T01 MTR_4g064570 44.000 75 36 1 28 102 154 222 9.07e-17 77.4
MsG0280009395.01.T01 MTR_6g016350 53.704 54 25 0 59 112 309 362 2.86e-16 75.9
MsG0280009395.01.T01 MTR_5g088720 41.892 74 37 1 29 102 174 241 3.50e-15 72.4
MsG0280009395.01.T01 MTR_5g088720 41.892 74 37 1 29 102 179 246 3.62e-15 72.4
MsG0280009395.01.T01 MTR_5g088720 41.892 74 37 1 29 102 220 287 4.33e-15 72.4
MsG0280009395.01.T01 MTR_3g072630 38.947 95 45 3 15 102 174 262 9.60e-15 71.2
MsG0280009395.01.T01 MTR_3g083480 37.805 82 40 1 28 109 215 285 9.90e-15 71.2
MsG0280009395.01.T01 MTR_2g007340 39.796 98 50 3 8 102 151 242 1.34e-14 70.9
MsG0280009395.01.T01 MTR_3g072630 38.947 95 45 3 15 102 174 262 1.49e-14 70.9
MsG0280009395.01.T01 MTR_5g080350 34.783 92 53 2 29 120 168 252 7.34e-14 68.9
MsG0280009395.01.T01 MTR_3g068005 35.294 85 49 1 29 113 170 248 4.94e-13 66.6
MsG0280009395.01.T01 MTR_1g014710 32.500 80 51 1 29 108 204 280 6.20e-13 66.2
MsG0280009395.01.T01 MTR_6g032960 29.825 114 62 2 6 112 185 287 7.75e-12 63.2
MsG0280009395.01.T01 MTR_1g051105 36.486 74 41 2 29 102 133 200 8.06e-12 62.4
MsG0280009395.01.T01 MTR_0067s0100 35.616 73 41 1 30 102 165 231 2.12e-11 61.6
MsG0280009395.01.T01 MTR_1g011380 39.655 58 35 0 60 117 340 397 6.67e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009395.01.T01 AT3G60080 39.316 117 52 3 38 143 144 252 4.35e-18 80.5
MsG0280009395.01.T01 AT5G01980 41.304 92 48 2 28 119 323 408 7.22e-17 77.8
MsG0280009395.01.T01 AT5G15820 44.000 75 37 1 28 102 263 332 9.84e-17 77.0
MsG0280009395.01.T01 AT3G56580 44.595 74 36 1 29 102 159 227 1.96e-16 75.9
MsG0280009395.01.T01 AT3G56580 44.595 74 36 1 29 102 159 227 1.96e-16 75.9
MsG0280009395.01.T01 AT3G56580 44.595 74 36 1 29 102 159 227 1.96e-16 75.9
MsG0280009395.01.T01 AT5G08139 34.783 92 50 2 16 102 264 350 4.13e-16 75.5
MsG0280009395.01.T01 AT2G40830 45.946 74 35 2 29 102 163 231 9.80e-16 73.9
MsG0280009395.01.T01 AT2G40830 45.946 74 35 2 29 102 163 231 9.80e-16 73.9
MsG0280009395.01.T01 AT2G40830 45.946 74 35 2 29 102 163 231 9.80e-16 73.9
MsG0280009395.01.T01 AT2G40830 45.946 74 35 2 29 102 163 231 9.80e-16 73.9
MsG0280009395.01.T01 AT5G59550 45.588 68 35 1 61 126 304 371 1.37e-15 74.3
MsG0280009395.01.T01 AT3G02340 41.463 82 43 2 28 109 307 383 1.38e-15 73.9
MsG0280009395.01.T01 AT3G02340 41.463 82 43 2 28 109 307 383 1.38e-15 73.9
MsG0280009395.01.T01 AT1G60360 37.037 108 57 2 29 130 197 299 1.65e-15 73.6
MsG0280009395.01.T01 AT5G59550 36.000 100 56 2 29 126 173 266 1.93e-15 73.6
MsG0280009395.01.T01 AT2G39720 43.243 74 38 1 29 102 173 242 4.72e-15 72.4
MsG0280009395.01.T01 AT2G39720 43.243 74 38 1 29 102 173 242 4.72e-15 72.4
MsG0280009395.01.T01 AT3G19950 36.364 88 51 1 28 115 188 270 5.24e-15 72.0
MsG0280009395.01.T01 AT3G19950 36.782 87 50 1 28 114 246 327 5.54e-15 72.4
MsG0280009395.01.T01 AT3G19950 36.782 87 50 1 28 114 246 327 5.54e-15 72.4
MsG0280009395.01.T01 AT3G46620 33.673 98 59 1 29 126 189 280 8.39e-15 72.0
MsG0280009395.01.T01 AT2G44330 44.615 65 36 0 38 102 73 137 3.57e-14 67.8
MsG0280009395.01.T01 AT1G55530 37.805 82 40 1 28 109 201 271 4.03e-14 69.7
MsG0280009395.01.T01 AT1G55530 37.805 82 40 1 28 109 201 271 4.03e-14 69.7
MsG0280009395.01.T01 AT1G55530 37.805 82 40 1 28 109 168 238 4.28e-14 69.3
MsG0280009395.01.T01 AT5G64920 47.458 59 31 0 58 116 254 312 4.65e-14 69.3
MsG0280009395.01.T01 AT3G30460 46.939 49 26 0 57 105 129 177 7.17e-14 67.0
MsG0280009395.01.T01 AT3G30460 50.000 46 23 0 57 102 93 138 7.43e-14 66.2
MsG0280009395.01.T01 AT5G60820 38.667 75 44 1 28 102 341 413 9.49e-14 68.9
MsG0280009395.01.T01 AT5G56340 32.692 104 57 2 28 131 237 327 1.12e-13 68.6
MsG0280009395.01.T01 AT3G13430 36.000 75 38 1 28 102 202 266 6.43e-13 66.2
MsG0280009395.01.T01 AT3G13430 36.000 75 38 1 28 102 202 266 6.43e-13 66.2
MsG0280009395.01.T01 AT3G13430 36.000 75 38 1 28 102 202 266 6.43e-13 66.2
MsG0280009395.01.T01 AT3G13430 36.000 75 38 1 28 102 202 266 6.43e-13 66.2
MsG0280009395.01.T01 AT3G10815 33.735 83 49 2 29 111 95 171 9.14e-13 64.3
MsG0280009395.01.T01 AT4G26400 33.333 81 43 1 30 110 221 290 1.73e-12 65.1
MsG0280009395.01.T01 AT4G26400 33.333 81 43 1 30 110 221 290 1.73e-12 65.1
MsG0280009395.01.T01 AT1G68180 41.892 74 37 1 29 102 112 179 2.03e-12 63.9
MsG0280009395.01.T01 AT5G20910 27.679 112 64 2 28 125 200 308 5.78e-12 63.2
MsG0280009395.01.T01 AT4G32600 37.500 56 35 0 60 115 362 417 1.81e-11 62.4
MsG0280009395.01.T01 AT4G32600 37.500 56 35 0 60 115 362 417 1.81e-11 62.4
MsG0280009395.01.T01 AT3G55530 37.500 64 37 1 59 119 209 272 5.34e-11 60.5
MsG0280009395.01.T01 AT1G26800 28.421 95 68 0 26 120 78 172 8.83e-11 58.9

Find 49 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCTTCATTCTAATGATTC+TGG 0.212324 2:-51290351 MsG0280009395.01.T01:CDS
ATGAAGAATCGGCGTCGTTT+TGG 0.271107 2:+51290799 None:intergenic
TACTGTAGGGATTGTTGTTT+CGG 0.304144 2:+51290713 None:intergenic
GAGCTTCTTGTCCTCTTTGT+CGG 0.339530 2:-51290533 MsG0280009395.01.T01:CDS
GATGAGGAAGATGATGTTAA+TGG 0.351942 2:-51290477 MsG0280009395.01.T01:CDS
GGCGGTGGTGGTGGTGTAGA+TGG 0.381074 2:-51290291 MsG0280009395.01.T01:CDS
ATTGTATTACTCCGTGGCTT+CGG 0.399609 2:-51290560 MsG0280009395.01.T01:CDS
AGTGAACTTGGCGGCGGTGG+TGG 0.415179 2:-51290303 MsG0280009395.01.T01:CDS
GAACTTGGCGGCGGTGGTGG+TGG 0.437033 2:-51290300 MsG0280009395.01.T01:CDS
CATTCTAATGATTCTGGTGG+TGG 0.447523 2:-51290345 MsG0280009395.01.T01:CDS
AGGGATTGTTGTTTCGGTGC+GGG 0.459559 2:+51290719 None:intergenic
GCCGTTTGAGGGATCGAGTA+AGG 0.460625 2:+51290668 None:intergenic
ACCTTACTCGATCCCTCAAA+CGG 0.473396 2:-51290669 MsG0280009395.01.T01:CDS
GCTAGTGAACTTGGCGGCGG+TGG 0.473653 2:-51290306 MsG0280009395.01.T01:CDS
GCATATAACGACGCCGTTTG+AGG 0.475721 2:+51290656 None:intergenic
GTTTCGGTGCGGGATTTAGC+GGG 0.479527 2:+51290729 None:intergenic
TCTTTGTCGGTGCGGAATTG+TGG 0.480140 2:-51290520 MsG0280009395.01.T01:CDS
GGAAACCGTTTCTTTACAAA+CGG 0.482757 2:+51290632 None:intergenic
CATATAACGACGCCGTTTGA+GGG 0.500879 2:+51290657 None:intergenic
CTGTTTCGCTTCCTCGTCAA+CGG 0.504931 2:+51290611 None:intergenic
ATGCGCCGTTTGTAAAGAAA+CGG 0.509933 2:-51290637 MsG0280009395.01.T01:CDS
GTCGAAGAAAGATGAAGAAT+CGG 0.511489 2:+51290788 None:intergenic
AGTGATGATGCTAGTGAACT+TGG 0.519447 2:-51290315 MsG0280009395.01.T01:CDS
GGAGATGGTGATGCGCTTGT+CGG 0.522674 2:-51290439 MsG0280009395.01.T01:CDS
TTTGTGTGACGGTTACTGTA+GGG 0.534278 2:+51290700 None:intergenic
TGTTTCGGTGCGGGATTTAG+CGG 0.546426 2:+51290728 None:intergenic
TGAGTAGTAAAAGCGCGTCT+TGG 0.555119 2:+51290757 None:intergenic
TTGTGATCATCTTTATCATG+CGG 0.559253 2:-51290583 MsG0280009395.01.T01:CDS
GGTAAGAAGCGTTTGTGTGA+CGG 0.566304 2:+51290689 None:intergenic
CGGAATTGTGGAGGATGAAG+AGG 0.572347 2:-51290508 MsG0280009395.01.T01:CDS
GTTTGTGTGACGGTTACTGT+AGG 0.576938 2:+51290699 None:intergenic
ACAATTCCGCACCGACAAAG+AGG 0.599284 2:+51290522 None:intergenic
GAGGAAGATGATGTTAATGG+TGG 0.599878 2:-51290474 MsG0280009395.01.T01:CDS
TAGGGATTGTTGTTTCGGTG+CGG 0.602770 2:+51290718 None:intergenic
TGAAGAGGATGTGAATGATG+AGG 0.616785 2:-51290493 MsG0280009395.01.T01:CDS
CTTCATTCTAATGATTCTGG+TGG 0.625157 2:-51290348 MsG0280009395.01.T01:CDS
GAAGCTCGAATCCGAAGCCA+CGG 0.626516 2:+51290549 None:intergenic
TTGTCGGTGCGGAATTGTGG+AGG 0.626660 2:-51290517 MsG0280009395.01.T01:CDS
TCTTGTCCTCTTTGTCGGTG+CGG 0.626689 2:-51290528 MsG0280009395.01.T01:CDS
GATGATGCTAGTGAACTTGG+CGG 0.626692 2:-51290312 MsG0280009395.01.T01:CDS
TCGGTGCGGGATTTAGCGGG+AGG 0.626805 2:+51290732 None:intergenic
TAATGGTGGAGATGTGATGA+GGG 0.627088 2:-51290460 MsG0280009395.01.T01:CDS
AGAAACGGTTTCCGTTGACG+AGG 0.647530 2:-51290622 MsG0280009395.01.T01:CDS
GATGCTAGTGAACTTGGCGG+CGG 0.650900 2:-51290309 MsG0280009395.01.T01:CDS
GCGCTTGTCGGAGTTGTCTG+AGG 0.656779 2:-51290427 MsG0280009395.01.T01:CDS
TGGAGATGTGATGAGGGAGA+TGG 0.658931 2:-51290454 MsG0280009395.01.T01:CDS
ATGCGGATTGTATTACTCCG+TGG 0.659920 2:-51290566 MsG0280009395.01.T01:CDS
TTAATGGTGGAGATGTGATG+AGG 0.684637 2:-51290461 MsG0280009395.01.T01:CDS
GCATGATAAAGATGATCACA+AGG 0.688727 2:+51290585 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTGTGATCATCTTTATCATG+CGG - Chr2:51290511-51290530 MsG0280009395.01.T01:CDS 30.0%
! TTGTCTGAGGATGATTTTTA+TGG - Chr2:51290680-51290699 MsG0280009395.01.T01:CDS 30.0%
!! AGGATGATTTTTATGGATTG+AGG - Chr2:51290687-51290706 MsG0280009395.01.T01:CDS 30.0%
CTGCTTCATTCTAATGATTC+TGG - Chr2:51290743-51290762 MsG0280009395.01.T01:CDS 35.0%
CTTCATTCTAATGATTCTGG+TGG - Chr2:51290746-51290765 MsG0280009395.01.T01:CDS 35.0%
GATGAGGAAGATGATGTTAA+TGG - Chr2:51290617-51290636 MsG0280009395.01.T01:CDS 35.0%
GCATGATAAAGATGATCACA+AGG + Chr2:51290512-51290531 None:intergenic 35.0%
GGAAACCGTTTCTTTACAAA+CGG + Chr2:51290465-51290484 None:intergenic 35.0%
GTCGAAGAAAGATGAAGAAT+CGG + Chr2:51290309-51290328 None:intergenic 35.0%
!! TACTGTAGGGATTGTTGTTT+CGG + Chr2:51290384-51290403 None:intergenic 35.0%
ATGCGCCGTTTGTAAAGAAA+CGG - Chr2:51290457-51290476 MsG0280009395.01.T01:CDS 40.0%
ATTGTATTACTCCGTGGCTT+CGG - Chr2:51290534-51290553 MsG0280009395.01.T01:CDS 40.0%
CATTCTAATGATTCTGGTGG+TGG - Chr2:51290749-51290768 MsG0280009395.01.T01:CDS 40.0%
GAGGAAGATGATGTTAATGG+TGG - Chr2:51290620-51290639 MsG0280009395.01.T01:CDS 40.0%
TGAAGAGGATGTGAATGATG+AGG - Chr2:51290601-51290620 MsG0280009395.01.T01:CDS 40.0%
TTTGTGTGACGGTTACTGTA+GGG + Chr2:51290397-51290416 None:intergenic 40.0%
! AGTGATGATGCTAGTGAACT+TGG - Chr2:51290779-51290798 MsG0280009395.01.T01:CDS 40.0%
! TAATGGTGGAGATGTGATGA+GGG - Chr2:51290634-51290653 MsG0280009395.01.T01:CDS 40.0%
! TTAATGGTGGAGATGTGATG+AGG - Chr2:51290633-51290652 MsG0280009395.01.T01:CDS 40.0%
ACCTTACTCGATCCCTCAAA+CGG - Chr2:51290425-51290444 MsG0280009395.01.T01:CDS 45.0%
CATATAACGACGCCGTTTGA+GGG + Chr2:51290440-51290459 None:intergenic 45.0%
GAGCTTCTTGTCCTCTTTGT+CGG - Chr2:51290561-51290580 MsG0280009395.01.T01:CDS 45.0%
GATGATGCTAGTGAACTTGG+CGG - Chr2:51290782-51290801 MsG0280009395.01.T01:CDS 45.0%
GTTTGTGTGACGGTTACTGT+AGG + Chr2:51290398-51290417 None:intergenic 45.0%
! ATGAAGAATCGGCGTCGTTT+TGG + Chr2:51290298-51290317 None:intergenic 45.0%
! ATGCGGATTGTATTACTCCG+TGG - Chr2:51290528-51290547 MsG0280009395.01.T01:CDS 45.0%
! TGAGTAGTAAAAGCGCGTCT+TGG + Chr2:51290340-51290359 None:intergenic 45.0%
!! GGTAAGAAGCGTTTGTGTGA+CGG + Chr2:51290408-51290427 None:intergenic 45.0%
!! TAGGGATTGTTGTTTCGGTG+CGG + Chr2:51290379-51290398 None:intergenic 45.0%
!! TGAAGAATCGGCGTCGTTTT+GGG + Chr2:51290297-51290316 None:intergenic 45.0%
AGAAACGGTTTCCGTTGACG+AGG - Chr2:51290472-51290491 MsG0280009395.01.T01:CDS 50.0%
CGGAATTGTGGAGGATGAAG+AGG - Chr2:51290586-51290605 MsG0280009395.01.T01:CDS 50.0%
CTGTTTCGCTTCCTCGTCAA+CGG + Chr2:51290486-51290505 None:intergenic 50.0%
GCATATAACGACGCCGTTTG+AGG + Chr2:51290441-51290460 None:intergenic 50.0%
TCTTGTCCTCTTTGTCGGTG+CGG - Chr2:51290566-51290585 MsG0280009395.01.T01:CDS 50.0%
TCTTTGTCGGTGCGGAATTG+TGG - Chr2:51290574-51290593 MsG0280009395.01.T01:CDS 50.0%
TGGAGATGTGATGAGGGAGA+TGG - Chr2:51290640-51290659 MsG0280009395.01.T01:CDS 50.0%
! ACAATTCCGCACCGACAAAG+AGG + Chr2:51290575-51290594 None:intergenic 50.0%
! TGTTTCGGTGCGGGATTTAG+CGG + Chr2:51290369-51290388 None:intergenic 50.0%
!! AGGGATTGTTGTTTCGGTGC+GGG + Chr2:51290378-51290397 None:intergenic 50.0%
!!! TCGGCGTCGTTTTGGGAAAA+AGG + Chr2:51290290-51290309 None:intergenic 50.0%
GAAGCTCGAATCCGAAGCCA+CGG + Chr2:51290548-51290567 None:intergenic 55.0%
GATGCTAGTGAACTTGGCGG+CGG - Chr2:51290785-51290804 MsG0280009395.01.T01:CDS 55.0%
GCCGTTTGAGGGATCGAGTA+AGG + Chr2:51290429-51290448 None:intergenic 55.0%
TTGTCGGTGCGGAATTGTGG+AGG - Chr2:51290577-51290596 MsG0280009395.01.T01:CDS 55.0%
! GGAGATGGTGATGCGCTTGT+CGG - Chr2:51290655-51290674 MsG0280009395.01.T01:CDS 55.0%
! GTTTCGGTGCGGGATTTAGC+GGG + Chr2:51290368-51290387 None:intergenic 55.0%
GCGCTTGTCGGAGTTGTCTG+AGG - Chr2:51290667-51290686 MsG0280009395.01.T01:CDS 60.0%
AGTGAACTTGGCGGCGGTGG+TGG - Chr2:51290791-51290810 MsG0280009395.01.T01:CDS 65.0%
GCTAGTGAACTTGGCGGCGG+TGG - Chr2:51290788-51290807 MsG0280009395.01.T01:CDS 65.0%
TCGGTGCGGGATTTAGCGGG+AGG + Chr2:51290365-51290384 None:intergenic 65.0%
!! GGCGGTGGTGGTGGTGTAGA+TGG - Chr2:51290803-51290822 MsG0280009395.01.T01:CDS 65.0%
! GAACTTGGCGGCGGTGGTGG+TGG - Chr2:51290794-51290813 MsG0280009395.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr2 gene 51290278 51290838 51290278 ID=MsG0280009395.01;Name=MsG0280009395.01
Chr2 mRNA 51290278 51290838 51290278 ID=MsG0280009395.01.T01;Parent=MsG0280009395.01;Name=MsG0280009395.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|186
Chr2 exon 51290278 51290838 51290278 ID=MsG0280009395.01.T01:exon:9049;Parent=MsG0280009395.01.T01
Chr2 CDS 51290278 51290838 51290278 ID=MsG0280009395.01.T01:cds;Parent=MsG0280009395.01.T01
Gene Sequence

>MsG0280009395.01.T01

ATGGACTCACCTTTTTCCCAAAACGACGCCGATTCTTCATCTTTCTTCGACGTTGTTTTCCAAGACGCGCTTTTACTACTCACACCTCCCGCTAAATCCCGCACCGAAACAACAATCCCTACAGTAACCGTCACACAAACGCTTCTTACCTTACTCGATCCCTCAAACGGCGTCGTTATATGCGCCGTTTGTAAAGAAACGGTTTCCGTTGACGAGGAAGCGAAACAGCTTCCTTGTGATCATCTTTATCATGCGGATTGTATTACTCCGTGGCTTCGGATTCGAGCTTCTTGTCCTCTTTGTCGGTGCGGAATTGTGGAGGATGAAGAGGATGTGAATGATGAGGAAGATGATGTTAATGGTGGAGATGTGATGAGGGAGATGGTGATGCGCTTGTCGGAGTTGTCTGAGGATGATTTTTATGGATTGAGGATTACTCTGAATCACATTGCTTCACGTCATGCTCTGCTTCATTCTAATGATTCTGGTGGTGGTGAGAATAGTGATGATGCTAGTGAACTTGGCGGCGGTGGTGGTGGTGTAGATGGAGAAGAATCTTAA

Protein sequence

>MsG0280009395.01.T01

MDSPFSQNDADSSSFFDVVFQDALLLLTPPAKSRTETTIPTVTVTQTLLTLLDPSNGVVICAVCKETVSVDEEAKQLPCDHLYHADCITPWLRIRASCPLCRCGIVEDEEDVNDEEDDVNGGDVMREMVMRLSELSEDDFYGLRITLNHIASRHALLHSNDSGGGENSDDASELGGGGGGVDGEES*