AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004417.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004417.01.T01 MTR_1g079370 69.434 265 15 2 1 199 1 265 3.50e-120 341
MsG0180004417.01.T01 MTR_1g079380 63.019 265 32 2 1 199 1 265 2.55e-97 285
MsG0180004417.01.T01 MTR_1g079180 56.716 268 44 5 1 199 1 265 1.47e-94 276
MsG0180004417.01.T01 MTR_1g100597 54.779 272 50 3 1 199 1 272 3.26e-91 268
MsG0180004417.01.T01 MTR_1g079260 52.574 272 56 3 1 199 1 272 2.49e-89 263
MsG0180004417.01.T01 MTR_1g079200 54.340 265 54 3 1 199 1 264 8.99e-87 256
MsG0180004417.01.T01 MTR_1g079460 50.376 266 65 3 1 199 1 266 1.24e-80 241
MsG0180004417.01.T01 MTR_1g079230 47.985 273 68 4 1 199 1 273 1.28e-77 234
MsG0180004417.01.T01 MTR_1g079850 48.043 281 64 5 1 199 1 281 7.67e-71 217
MsG0180004417.01.T01 MTR_1g100613 60.106 188 38 2 15 166 1 187 1.15e-70 215
MsG0180004417.01.T01 MTR_1g079250 47.842 278 66 5 1 199 1 278 1.55e-69 214
MsG0180004417.01.T01 MTR_1g026510 43.463 283 76 6 1 199 1 283 4.20e-66 205
MsG0180004417.01.T01 MTR_3g452600 44.615 260 78 4 4 199 10 267 4.73e-63 196
MsG0180004417.01.T01 MTR_3g006850 44.147 299 64 7 4 199 17 315 1.94e-62 196
MsG0180004417.01.T01 MTR_1g079350 42.909 275 81 6 1 199 1 275 1.42e-55 177
MsG0180004417.01.T01 MTR_1g079410 41.573 267 84 5 4 199 8 273 1.18e-54 175
MsG0180004417.01.T01 MTR_3g450990 40.942 276 81 5 4 199 10 283 6.24e-54 174
MsG0180004417.01.T01 MTR_2g079100 43.295 261 72 5 4 196 9 261 9.25e-54 172
MsG0180004417.01.T01 MTR_1g079140 60.000 145 52 2 3 142 12 155 7.02e-53 172
MsG0180004417.01.T01 MTR_1g079400 41.045 268 86 5 4 199 12 279 9.92e-53 170
MsG0180004417.01.T01 MTR_1g079340 38.811 286 88 7 1 199 40 325 3.72e-46 155
MsG0180004417.01.T01 MTR_1g079350 60.902 133 47 2 1 128 1 133 7.15e-46 151
MsG0180004417.01.T01 MTR_7g096300 47.317 205 59 5 1 163 1 198 1.49e-43 154
MsG0180004417.01.T01 MTR_1g078460 71.111 90 26 0 1 90 64 153 2.14e-38 130
MsG0180004417.01.T01 MTR_2g042540 64.762 105 35 2 1 103 1 105 3.96e-38 128
MsG0180004417.01.T01 MTR_1g083580 43.382 136 74 1 2 134 6 141 2.87e-35 125
MsG0180004417.01.T01 MTR_1g078470 42.941 170 32 3 93 199 17 184 5.02e-35 122
MsG0180004417.01.T01 MTR_7g112550 43.373 166 83 4 2 164 5 162 6.97e-35 124
MsG0180004417.01.T01 MTR_7g112560 32.558 258 105 8 7 199 4 257 5.64e-33 119
MsG0180004417.01.T01 MTR_4g023570 33.333 237 111 6 7 199 4 237 1.41e-32 117
MsG0180004417.01.T01 MTR_1g079240 40.588 170 46 5 85 199 10 179 2.55e-27 102
MsG0180004417.01.T01 MTR_0113s0040 36.296 135 83 2 2 134 20 153 1.25e-19 84.7
MsG0180004417.01.T01 MTR_7g103380 33.083 133 86 2 4 134 19 150 1.68e-19 84.3
MsG0180004417.01.T01 MTR_7g096310 45.536 112 43 4 38 142 19 119 2.84e-18 78.6
MsG0180004417.01.T01 MTR_1g101390 36.296 135 83 2 2 134 26 159 7.15e-18 79.7
MsG0180004417.01.T01 MTR_4g102770 35.075 134 83 2 4 134 19 151 9.32e-17 76.6
MsG0180004417.01.T01 MTR_5g010800 33.813 139 81 5 2 134 15 148 5.78e-16 74.7
MsG0180004417.01.T01 MTR_0002s0020 31.655 139 87 2 3 134 21 158 1.58e-15 73.6
MsG0180004417.01.T01 MTR_1g050352 37.415 147 45 5 24 165 44 148 6.79e-15 69.3
MsG0180004417.01.T01 MTR_1g079130 66.667 42 14 0 101 142 1 42 8.63e-12 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004417.01.T01 AT5G24560 44.103 195 101 3 6 199 5 192 1.61e-51 164
MsG0180004417.01.T01 AT2G02360 35.686 255 98 5 7 198 17 268 7.56e-46 152
MsG0180004417.01.T01 AT1G80110 52.273 132 60 1 4 135 1 129 2.91e-43 145
MsG0180004417.01.T01 AT2G02240 34.375 256 101 4 7 198 64 316 4.66e-42 144
MsG0180004417.01.T01 AT2G02250 35.000 260 101 6 4 198 47 303 9.00e-39 135
MsG0180004417.01.T01 AT2G02230 45.588 136 71 1 7 142 35 167 5.65e-38 134
MsG0180004417.01.T01 AT2G02340 44.366 142 78 1 1 142 33 173 6.13e-36 128
MsG0180004417.01.T01 AT1G09155 40.881 159 85 3 7 164 3 153 5.26e-33 120
MsG0180004417.01.T01 AT1G56250 31.900 279 103 6 7 199 4 281 5.06e-30 112
MsG0180004417.01.T01 AT2G02310 28.517 263 118 5 4 199 45 304 1.28e-29 111
MsG0180004417.01.T01 AT2G02350 31.298 262 110 6 4 198 24 282 4.47e-29 109
MsG0180004417.01.T01 AT2G02320 31.179 263 105 8 6 198 42 298 5.60e-28 107
MsG0180004417.01.T01 AT1G56240 38.994 159 88 3 7 164 4 154 2.75e-26 102
MsG0180004417.01.T01 AT2G02300 32.143 252 111 8 4 199 34 281 7.94e-26 101
MsG0180004417.01.T01 AT3G53000 36.364 132 82 2 4 134 21 151 6.44e-23 93.6
MsG0180004417.01.T01 AT3G61060 35.385 130 79 3 6 132 27 154 1.84e-17 79.0
MsG0180004417.01.T01 AT3G61060 33.846 130 82 2 6 132 27 155 5.03e-17 77.8
MsG0180004417.01.T01 AT1G12710 35.075 134 81 3 4 134 28 158 1.31e-16 76.6
MsG0180004417.01.T01 AT1G12710 33.582 134 83 3 4 134 28 158 3.82e-15 72.8
MsG0180004417.01.T01 AT1G63090 34.615 130 83 2 4 132 26 154 6.48e-15 71.6
MsG0180004417.01.T01 AT5G52120 31.690 142 88 3 1 134 18 158 4.28e-13 66.6

Find 49 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAGATCTCTTGCAATTGTT+TGG 0.246175 1:-77646987 MsG0180004417.01.T01:CDS
TAGATCTCTTGCAATTGTTT+GGG 0.285923 1:-77646986 MsG0180004417.01.T01:CDS
TGGAGATAAAGGCTCTTAAT+TGG 0.287168 1:-77645303 MsG0180004417.01.T01:CDS
CATACCTTCCACTTCACATT+TGG 0.304270 1:+77645445 None:intergenic
GATGAGTTTGTTGGAGATAA+AGG 0.307324 1:-77645314 MsG0180004417.01.T01:CDS
TTCTTGAAGGAATAGAAATT+AGG 0.317502 1:-77645267 MsG0180004417.01.T01:CDS
TGCCAACGCTCCCACCAAAA+AGG 0.334601 1:-77647227 MsG0180004417.01.T01:CDS
GGATTCCTTTGCCTGATTCC+AGG 0.335609 1:-77646939 MsG0180004417.01.T01:intron
TCAACAAGCTTAGCAACTTC+AGG 0.357954 1:+77645645 None:intergenic
TGATCATGATCGATGATGAT+AGG 0.375392 1:+77647178 None:intergenic
CATCCGATTCCGATGCTGTA+TGG 0.419002 1:-77647303 MsG0180004417.01.T01:CDS
TAGTACTAAAATTGTCTGTT+TGG 0.420764 1:-77645470 MsG0180004417.01.T01:CDS
GACAGAGATTCAGGATTTGC+CGG 0.423087 1:-77647410 MsG0180004417.01.T01:CDS
AAGAGTGGTCTCTTTCTTGA+AGG 0.428845 1:-77645280 MsG0180004417.01.T01:CDS
ATACTAAGCACTATTGGAAC+TGG 0.440686 1:-77646960 MsG0180004417.01.T01:CDS
TGGAGTTGTACGAGAGACTA+TGG 0.442874 1:+77647372 None:intergenic
GGGGTGATACTAAGCACTAT+TGG 0.458105 1:-77646966 MsG0180004417.01.T01:CDS
GCGCTAACTAACCTGGAATC+AGG 0.472883 1:+77646928 None:intergenic
CATCATCGATCATGATCAAA+AGG 0.478451 1:-77647173 MsG0180004417.01.T01:CDS
AGAAGTCCTGATGAGTTTGT+TGG 0.481141 1:-77645323 MsG0180004417.01.T01:CDS
ATGGCGGCAATGCATCCTTC+CGG 0.482859 1:+77647391 None:intergenic
TTATCTCCAACAAACTCATC+AGG 0.495784 1:+77645317 None:intergenic
ATTCCATACAGCATCGGAAT+CGG 0.497032 1:+77647300 None:intergenic
TGCTGCTTAATGTGTTACTT+AGG 0.503827 1:+77645244 None:intergenic
GGATGCAGATCGGAAAGTCT+TGG 0.523752 1:+77647321 None:intergenic
CAACAAGCTTAGCAACTTCA+GGG 0.526604 1:+77645646 None:intergenic
GTGGGAGCGTTGGCAAGGGA+AGG 0.528441 1:+77647235 None:intergenic
TTTCAATTGGACAGAAAGAG+TGG 0.544181 1:-77647033 MsG0180004417.01.T01:CDS
CATCGGAATCGGATGCAGAT+CGG 0.545902 1:+77647311 None:intergenic
ATGATAGATGAGATGTCAGA+AGG 0.548763 1:+77647268 None:intergenic
AGATCTCTTGCAATTGTTTG+GGG 0.552612 1:-77646985 MsG0180004417.01.T01:CDS
GAGATTCAGGATTTGCCGGA+AGG 0.560627 1:-77647406 MsG0180004417.01.T01:CDS
GGGATTTCAGAACTGTCCTG+TGG 0.565853 1:-77645524 MsG0180004417.01.T01:intron
GAGAGTCTGCCGGCGTCGAG+TGG 0.572733 1:+77647352 None:intergenic
AAGGCTCTTAATTGGAAGAG+TGG 0.575926 1:-77645295 MsG0180004417.01.T01:CDS
TTCTCTAAGTCGATCTATGG+CGG 0.576702 1:+77647440 None:intergenic
TTTGGTGGGAGCGTTGGCAA+GGG 0.595797 1:+77647231 None:intergenic
GTTTGGATCCAAATGTGAAG+TGG 0.602262 1:-77645453 MsG0180004417.01.T01:CDS
TTATCAGTTGGTGTCGAAGG+TGG 0.605148 1:-77645499 MsG0180004417.01.T01:CDS
ACACCAACTGATAACTCCAC+AGG 0.607721 1:+77645508 None:intergenic
GGATCCAAATGTGAAGTGGA+AGG 0.615381 1:-77645449 MsG0180004417.01.T01:intron
GAGTTATCAGTTGGTGTCGA+AGG 0.620810 1:-77645502 MsG0180004417.01.T01:CDS
TGTCCTGTGGAGTTATCAGT+TGG 0.623846 1:-77645511 MsG0180004417.01.T01:CDS
AAATTGATTCCATACAGCAT+CGG 0.632315 1:+77647294 None:intergenic
ACTAACCTGGAATCAGGCAA+AGG 0.635048 1:+77646934 None:intergenic
GGAAACGACAGAGAGTCTGC+CGG 0.651097 1:+77647342 None:intergenic
CATCGATCATGATCAAAAGG+TGG 0.658698 1:-77647170 MsG0180004417.01.T01:intron
CGTACAACTCCACTCGACGC+CGG 0.717247 1:-77647361 MsG0180004417.01.T01:CDS
AGTTGTACGAGAGACTATGG+CGG 0.789727 1:+77647375 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTCAAAAAGAATAACAAAA+AGG - Chr1:77646020-77646039 MsG0180004417.01.T01:intron 15.0%
!! ATTTCTCGAATATAATATTA+AGG - Chr1:77645579-77645598 MsG0180004417.01.T01:intron 15.0%
!! TATTATATTCGAGAAATTGA+CGG + Chr1:77645576-77645595 None:intergenic 20.0%
!!! GTAAAATTTCTAAGAAAACA+TGG + Chr1:77646633-77646652 None:intergenic 20.0%
!!! TGGTCAGATATTTTAAAATT+TGG + Chr1:77646468-77646487 None:intergenic 20.0%
! AATAGCCTCTAGTATTAATT+AGG + Chr1:77645842-77645861 None:intergenic 25.0%
! GATGCATTCAAAATCATATA+GGG + Chr1:77646439-77646458 None:intergenic 25.0%
! GATTGAACATGCTTAAATTT+GGG + Chr1:77646190-77646209 None:intergenic 25.0%
! GTTGTAAAGTAACAAACAAT+TGG + Chr1:77646488-77646507 None:intergenic 25.0%
! TAACTCCTAATTAATACTAG+AGG - Chr1:77645834-77645853 MsG0180004417.01.T01:intron 25.0%
! TAGTACTAAAATTGTCTGTT+TGG - Chr1:77647235-77647254 MsG0180004417.01.T01:CDS 25.0%
! TCAAATATAACGGTTTGATT+CGG + Chr1:77645874-77645893 None:intergenic 25.0%
! TGATGCATTCAAAATCATAT+AGG + Chr1:77646440-77646459 None:intergenic 25.0%
! TGATTGAACATGCTTAAATT+TGG + Chr1:77646191-77646210 None:intergenic 25.0%
! TGCTATTAGCTCAAATATAA+CGG + Chr1:77645884-77645903 None:intergenic 25.0%
! TTCTTGAAGGAATAGAAATT+AGG - Chr1:77647438-77647457 MsG0180004417.01.T01:five_prime_UTR 25.0%
!! CAAATTTTCAGAGTTTCAAT+TGG - Chr1:77645659-77645678 MsG0180004417.01.T01:intron 25.0%
!! TTGAAACTCTGAAAATTTGA+AGG + Chr1:77645658-77645677 None:intergenic 25.0%
!!! TAAGTTATATATGCCGTTTT+AGG + Chr1:77646861-77646880 None:intergenic 25.0%
AAAAAGAATGACAGAGATTC+AGG - Chr1:77645286-77645305 MsG0180004417.01.T01:CDS 30.0%
AACCTTATGCAAAGATTGAA+TGG + Chr1:77646689-77646708 None:intergenic 30.0%
AATTGATCACGTCCATAAAA+AGG + Chr1:77645979-77645998 None:intergenic 30.0%
ACGTGATCAATTAATGTTGA+TGG - Chr1:77645987-77646006 MsG0180004417.01.T01:intron 30.0%
AGTGTTTATTACATGACAGA+TGG + Chr1:77646975-77646994 None:intergenic 30.0%
ATAAGCTGCATATTGAGTAT+TGG + Chr1:77647135-77647154 None:intergenic 30.0%
CATACCATATATCAAATCCA+TGG - Chr1:77646138-77646157 MsG0180004417.01.T01:intron 30.0%
CATGGATTTGATATATGGTA+TGG + Chr1:77646140-77646159 None:intergenic 30.0%
CATTCAAAATCATATAGGGT+CGG + Chr1:77646435-77646454 None:intergenic 30.0%
CGTGATCAATTAATGTTGAT+GGG - Chr1:77645988-77646007 MsG0180004417.01.T01:intron 30.0%
GAATGTTGGATTTCCTAAAA+CGG - Chr1:77646845-77646864 MsG0180004417.01.T01:intron 30.0%
GATTGTCACAAAATAAGAGT+TGG + Chr1:77646727-77646746 None:intergenic 30.0%
GCTTTGCTTGATAAAAATAG+TGG + Chr1:77645951-77645970 None:intergenic 30.0%
GTTCAAAATGATTGATGCTT+GGG - Chr1:77647160-77647179 MsG0180004417.01.T01:intron 30.0%
TAAGCTGCATATTGAGTATT+GGG + Chr1:77647134-77647153 None:intergenic 30.0%
TAGATCTCTTGCAATTGTTT+GGG - Chr1:77645719-77645738 MsG0180004417.01.T01:intron 30.0%
TGTTCAAAATGATTGATGCT+TGG - Chr1:77647159-77647178 MsG0180004417.01.T01:intron 30.0%
TTCAAAATGATTGATGCTTG+GGG - Chr1:77647161-77647180 MsG0180004417.01.T01:intron 30.0%
! AAATTGATTCCATACAGCAT+CGG + Chr1:77645414-77645433 None:intergenic 30.0%
! TCCTTTTAGGCTTTTAGTAT+GGG - Chr1:77646815-77646834 MsG0180004417.01.T01:intron 30.0%
! TTTTTCTCTAAGTCGATCTA+TGG + Chr1:77645271-77645290 None:intergenic 30.0%
!! AATTAATGTTGATGGGTTTC+AGG - Chr1:77645995-77646014 MsG0180004417.01.T01:intron 30.0%
!! AGACAATTTTAGTACTACTC+TGG + Chr1:77647232-77647251 None:intergenic 30.0%
!! ATTAATGTTGATGGGTTTCA+GGG - Chr1:77645996-77646015 MsG0180004417.01.T01:intron 30.0%
!! TTTGTGTACTACTTTTGAAC+AGG - Chr1:77647034-77647053 MsG0180004417.01.T01:CDS 30.0%
!!! TTTTTTGATCCTAATTGCTG+CGG - Chr1:77646406-77646425 MsG0180004417.01.T01:intron 30.0%
AATGGCCAAGGTAATTTGAT+GGG + Chr1:77646671-77646690 None:intergenic 35.0%
AGATCTCTTGCAATTGTTTG+GGG - Chr1:77645720-77645739 MsG0180004417.01.T01:intron 35.0%
ATACTAAGCACTATTGGAAC+TGG - Chr1:77645745-77645764 MsG0180004417.01.T01:intron 35.0%
ATGATAGATGAGATGTCAGA+AGG + Chr1:77645440-77645459 None:intergenic 35.0%
CACAAAATAAGAGTTGGAGT+TGG + Chr1:77646721-77646740 None:intergenic 35.0%
CAGTTAATTCTTAGCAGATG+AGG + Chr1:77646061-77646080 None:intergenic 35.0%
CATCATCGATCATGATCAAA+AGG - Chr1:77645532-77645551 MsG0180004417.01.T01:intron 35.0%
CTAGATCTCTTGCAATTGTT+TGG - Chr1:77645718-77645737 MsG0180004417.01.T01:intron 35.0%
CTAGCCATGGATTTGATATA+TGG + Chr1:77646145-77646164 None:intergenic 35.0%
GAAATCCCATCAAATTACCT+TGG - Chr1:77646663-77646682 MsG0180004417.01.T01:intron 35.0%
GATACTTAGCTGAAGTGATA+TGG + Chr1:77646274-77646293 None:intergenic 35.0%
TATTCGAGAAATTGACGGTT+AGG + Chr1:77645571-77645590 None:intergenic 35.0%
TCACGTCCATAAAAAGGATA+TGG + Chr1:77645973-77645992 None:intergenic 35.0%
TCCAAATTCTCTAAGTCACA+AGG + Chr1:77646241-77646260 None:intergenic 35.0%
TCCCATACTAAAAGCCTAAA+AGG + Chr1:77646819-77646838 None:intergenic 35.0%
TCCTTGTGACTTAGAGAATT+TGG - Chr1:77646237-77646256 MsG0180004417.01.T01:intron 35.0%
TGATCATGATCGATGATGAT+AGG + Chr1:77645530-77645549 None:intergenic 35.0%
TGGAGATAAAGGCTCTTAAT+TGG - Chr1:77647402-77647421 MsG0180004417.01.T01:CDS 35.0%
TTATCTCCAACAAACTCATC+AGG + Chr1:77647391-77647410 None:intergenic 35.0%
TTATTACATGACAGATGGAG+TGG + Chr1:77646970-77646989 None:intergenic 35.0%
TTTCAATTGGACAGAAAGAG+TGG - Chr1:77645672-77645691 MsG0180004417.01.T01:intron 35.0%
! AAGCTGCATATTGAGTATTG+GGG + Chr1:77647133-77647152 None:intergenic 35.0%
! GCAAAGCCATATCCTTTTTA+TGG - Chr1:77645964-77645983 MsG0180004417.01.T01:intron 35.0%
! GTCCTTTTAGGCTTTTAGTA+TGG - Chr1:77646814-77646833 MsG0180004417.01.T01:intron 35.0%
!! GATGAGTTTGTTGGAGATAA+AGG - Chr1:77647391-77647410 MsG0180004417.01.T01:CDS 35.0%
AACTGCAGAGTTACAGAAGA+AGG + Chr1:77646365-77646384 None:intergenic 40.0%
AAGAGTGGTCTCTTTCTTGA+AGG - Chr1:77647425-77647444 MsG0180004417.01.T01:exon 40.0%
AAGGCTCTTAATTGGAAGAG+TGG - Chr1:77647410-77647429 MsG0180004417.01.T01:CDS 40.0%
AAGTTAAGCGCTAACTAACC+TGG + Chr1:77645787-77645806 None:intergenic 40.0%
ACACTTATCATGAGAACCTG+AGG - Chr1:77646774-77646793 MsG0180004417.01.T01:intron 40.0%
ACTTAGCTGAAGTGATATGG+AGG + Chr1:77646271-77646290 None:intergenic 40.0%
AGAAGTCCTGATGAGTTTGT+TGG - Chr1:77647382-77647401 MsG0180004417.01.T01:CDS 40.0%
ATGCAAAGATTGAATGGCCA+AGG + Chr1:77646683-77646702 None:intergenic 40.0%
CAACAAGCTTAGCAACTTCA+GGG + Chr1:77647062-77647081 None:intergenic 40.0%
CATACCTTCCACTTCACATT+TGG + Chr1:77647263-77647282 None:intergenic 40.0%
CATCGATCATGATCAAAAGG+TGG - Chr1:77645535-77645554 MsG0180004417.01.T01:intron 40.0%
CATGTAGTGCATCTTCAATG+TGG + Chr1:77646607-77646626 None:intergenic 40.0%
CCAAGTCAGTGAAGAATAGT+TGG - Chr1:77646100-77646119 MsG0180004417.01.T01:intron 40.0%
GAATGGCCAAGGTAATTTGA+TGG + Chr1:77646672-77646691 None:intergenic 40.0%
GAGTTACAGAAGAAGGATCT+TGG + Chr1:77646358-77646377 None:intergenic 40.0%
GATAGGGGAATTCTTCAGTT+CGG - Chr1:77647347-77647366 MsG0180004417.01.T01:CDS 40.0%
GGCCATTCAATCTTTGCATA+AGG - Chr1:77646684-77646703 MsG0180004417.01.T01:intron 40.0%
GTATGGGAGAAGATGAATGT+TGG - Chr1:77646831-77646850 MsG0180004417.01.T01:intron 40.0%
GTTTGGATCCAAATGTGAAG+TGG - Chr1:77647252-77647271 MsG0180004417.01.T01:CDS 40.0%
TCAACAAGCTTAGCAACTTC+AGG + Chr1:77647063-77647082 None:intergenic 40.0%
TCAGGTTCTCATGATAAGTG+TGG + Chr1:77646775-77646794 None:intergenic 40.0%
TTCTCTAAGTCGATCTATGG+CGG + Chr1:77645268-77645287 None:intergenic 40.0%
! AGCAATGTCGATGTCCTTTT+AGG - Chr1:77646802-77646821 MsG0180004417.01.T01:intron 40.0%
! ATTCCATACAGCATCGGAAT+CGG + Chr1:77645408-77645427 None:intergenic 40.0%
! CCAACTATTCTTCACTGACT+TGG + Chr1:77646103-77646122 None:intergenic 40.0%
! TAAATTTGGGTTTGAACCCG+AGG + Chr1:77646177-77646196 None:intergenic 40.0%
!! AGATACAGAGCCTTTTTGGT+GGG + Chr1:77645491-77645510 None:intergenic 40.0%
!! CTAAGCTTGTTGACGTATGT+TGG - Chr1:77647070-77647089 MsG0180004417.01.T01:intron 40.0%
!! GTATGTTGGCTTGAAATTCG+TGG - Chr1:77647084-77647103 MsG0180004417.01.T01:intron 40.0%
ACACCAACTGATAACTCCAC+AGG + Chr1:77647200-77647219 None:intergenic 45.0%
ACATAGGCCAGATTGGTGTA+AGG + Chr1:77646549-77646568 None:intergenic 45.0%
ACTAACCTGGAATCAGGCAA+AGG + Chr1:77645774-77645793 None:intergenic 45.0%
AGCTTCTACATAGGCCAGAT+TGG + Chr1:77646556-77646575 None:intergenic 45.0%
AGTTGTACGAGAGACTATGG+CGG + Chr1:77645333-77645352 None:intergenic 45.0%
ATGCAGCATAACAGAGCAGT+AGG - Chr1:77647280-77647299 MsG0180004417.01.T01:CDS 45.0%
CCAGATTGGTGTAAGGACTA+AGG + Chr1:77646542-77646561 None:intergenic 45.0%
CCTTAGTCCTTACACCAATC+TGG - Chr1:77646539-77646558 MsG0180004417.01.T01:intron 45.0%
GACAGAGATTCAGGATTTGC+CGG - Chr1:77645295-77645314 MsG0180004417.01.T01:CDS 45.0%
GGATCCAAATGTGAAGTGGA+AGG - Chr1:77647256-77647275 MsG0180004417.01.T01:CDS 45.0%
GGCCTAGTAAGCTTCTACAT+AGG + Chr1:77646565-77646584 None:intergenic 45.0%
GGCCTATGTAGAAGCTTACT+AGG - Chr1:77646560-77646579 MsG0180004417.01.T01:intron 45.0%
GGGGTGATACTAAGCACTAT+TGG - Chr1:77645739-77645758 MsG0180004417.01.T01:intron 45.0%
TGGAGTTGTACGAGAGACTA+TGG + Chr1:77645336-77645355 None:intergenic 45.0%
TGTCCTGTGGAGTTATCAGT+TGG - Chr1:77647194-77647213 MsG0180004417.01.T01:CDS 45.0%
! AGCGAGATACAGAGCCTTTT+TGG + Chr1:77645495-77645514 None:intergenic 45.0%
! GAGATACAGAGCCTTTTTGG+TGG + Chr1:77645492-77645511 None:intergenic 45.0%
! TTTTGCGTCCAACTTCAGCT+TGG - Chr1:77646328-77646347 MsG0180004417.01.T01:intron 45.0%
!! GAGTATTGGGGACAGAACAA+TGG + Chr1:77647121-77647140 None:intergenic 45.0%
!! GAGTTATCAGTTGGTGTCGA+AGG - Chr1:77647203-77647222 MsG0180004417.01.T01:CDS 45.0%
!! TTATCAGTTGGTGTCGAAGG+TGG - Chr1:77647206-77647225 MsG0180004417.01.T01:CDS 45.0%
AGCTTACTAGGCCACATGTG+TGG - Chr1:77646572-77646591 MsG0180004417.01.T01:intron 50.0%
ATGGCTAGTAGCTACCACCT+CGG - Chr1:77646157-77646176 MsG0180004417.01.T01:intron 50.0%
CATCCGATTCCGATGCTGTA+TGG - Chr1:77645402-77645421 MsG0180004417.01.T01:intron 50.0%
CATCGGAATCGGATGCAGAT+CGG + Chr1:77645397-77645416 None:intergenic 50.0%
GAGATTCAGGATTTGCCGGA+AGG - Chr1:77645299-77645318 MsG0180004417.01.T01:CDS 50.0%
GCGCTAACTAACCTGGAATC+AGG + Chr1:77645780-77645799 None:intergenic 50.0%
GGATGCAGATCGGAAAGTCT+TGG + Chr1:77645387-77645406 None:intergenic 50.0%
GGATTCCTTTGCCTGATTCC+AGG - Chr1:77645766-77645785 MsG0180004417.01.T01:intron 50.0%
GGGATTTCAGAACTGTCCTG+TGG - Chr1:77647181-77647200 MsG0180004417.01.T01:CDS 50.0%
GTCCTAGTGTGAGAAGCGAT+GGG - Chr1:77647311-77647330 MsG0180004417.01.T01:CDS 50.0%
TCGACATTGCTATGTGCCTC+AGG + Chr1:77646793-77646812 None:intergenic 50.0%
! ATTTGGGTTTGAACCCGAGG+TGG + Chr1:77646174-77646193 None:intergenic 50.0%
!! GGGTGGTGGAGATTGAGATA+GGG - Chr1:77647331-77647350 MsG0180004417.01.T01:CDS 50.0%
!! GGTGGTGGAGATTGAGATAG+GGG - Chr1:77647332-77647351 MsG0180004417.01.T01:CDS 50.0%
!! TGGGTGGTGGAGATTGAGAT+AGG - Chr1:77647330-77647349 MsG0180004417.01.T01:CDS 50.0%
ATGGCGGCAATGCATCCTTC+CGG + Chr1:77645317-77645336 None:intergenic 55.0%
CACCCATCGCTTCTCACACT+AGG + Chr1:77647316-77647335 None:intergenic 55.0%
CGTCCTAGTGTGAGAAGCGA+TGG - Chr1:77647310-77647329 MsG0180004417.01.T01:CDS 55.0%
CTAGTGTGAGAAGCGATGGG+TGG - Chr1:77647314-77647333 MsG0180004417.01.T01:CDS 55.0%
GAGGTGGTAGCTACTAGCCA+TGG + Chr1:77646158-77646177 None:intergenic 55.0%
GGAAACGACAGAGAGTCTGC+CGG + Chr1:77645366-77645385 None:intergenic 55.0%
GGATCCAGTGTCCACACATG+TGG + Chr1:77646586-77646605 None:intergenic 55.0%
TAGGCCACATGTGTGGACAC+TGG - Chr1:77646579-77646598 MsG0180004417.01.T01:intron 55.0%
TGCCAACGCTCCCACCAAAA+AGG - Chr1:77645478-77645497 MsG0180004417.01.T01:CDS 55.0%
TGGCTAGTAGCTACCACCTC+GGG - Chr1:77646158-77646177 MsG0180004417.01.T01:intron 55.0%
TTGGCGCTCCAAGCTGAAGT+TGG + Chr1:77646339-77646358 None:intergenic 55.0%
! GGTCGGTTTCCGCAGCAATT+AGG + Chr1:77646418-77646437 None:intergenic 55.0%
!! AGCCTTTTTGGTGGGAGCGT+TGG + Chr1:77645483-77645502 None:intergenic 55.0%
!! TTTGGTGGGAGCGTTGGCAA+GGG + Chr1:77645477-77645496 None:intergenic 55.0%
!!! TTTTGGTGGGAGCGTTGGCA+AGG + Chr1:77645478-77645497 None:intergenic 55.0%
CGTACAACTCCACTCGACGC+CGG - Chr1:77645344-77645363 MsG0180004417.01.T01:intron 60.0%
GTGTGAGAAGCGATGGGTGG+TGG - Chr1:77647317-77647336 MsG0180004417.01.T01:CDS 60.0%
!! GTGGGAGCGTTGGCAAGGGA+AGG + Chr1:77645473-77645492 None:intergenic 65.0%
! GAGAGTCTGCCGGCGTCGAG+TGG + Chr1:77645356-77645375 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 77645258 77647469 77645258 ID=MsG0180004417.01;Name=MsG0180004417.01
Chr1 mRNA 77645258 77647469 77645258 ID=MsG0180004417.01.T01;Parent=MsG0180004417.01;Name=MsG0180004417.01.T01;_AED=0.50;_eAED=0.50;_QI=35|0.25|0|1|1|1|5|0|199
Chr1 exon 77645258 77645362 77645258 ID=MsG0180004417.01.T01:exon:45198;Parent=MsG0180004417.01.T01
Chr1 exon 77645450 77645536 77645450 ID=MsG0180004417.01.T01:exon:45197;Parent=MsG0180004417.01.T01
Chr1 exon 77645644 77645671 77645644 ID=MsG0180004417.01.T01:exon:45196;Parent=MsG0180004417.01.T01
Chr1 exon 77646940 77647055 77646940 ID=MsG0180004417.01.T01:exon:45195;Parent=MsG0180004417.01.T01
Chr1 exon 77647171 77647469 77647171 ID=MsG0180004417.01.T01:exon:45194;Parent=MsG0180004417.01.T01
Chr1 five_prime_UTR 77647435 77647469 77647435 ID=MsG0180004417.01.T01:five_prime_utr;Parent=MsG0180004417.01.T01
Chr1 CDS 77647171 77647434 77647171 ID=MsG0180004417.01.T01:cds;Parent=MsG0180004417.01.T01
Chr1 CDS 77646940 77647055 77646940 ID=MsG0180004417.01.T01:cds;Parent=MsG0180004417.01.T01
Chr1 CDS 77645644 77645671 77645644 ID=MsG0180004417.01.T01:cds;Parent=MsG0180004417.01.T01
Chr1 CDS 77645450 77645536 77645450 ID=MsG0180004417.01.T01:cds;Parent=MsG0180004417.01.T01
Chr1 CDS 77645258 77645362 77645258 ID=MsG0180004417.01.T01:cds;Parent=MsG0180004417.01.T01
Gene Sequence

>MsG0180004417.01.T01

ATGACAGAGATTCAGGATTTGCCGGAAGGATGCATTGCCGCCATAGTCTCTCGTACAACTCCACTCGACGCCGGCAGACTCTCTGTCGTTTCCAAGACTTTCCGATCTGCATCCGATTCCGATGCTGTATGGAATCAATTTCTACCTTCTGACATCTCATCTATCATCTCACACTCTCCTTCCCTTGCCAACGCTCCCACCAAAAAGGCTCTGTATCTCGCTCTCTCTGATCGTCCTATCATCATCGATCATGATCAAAAGGTGTTTCAATTGGACAGAAAGAGTGGAAAAAAATGTTACATGCTTTCTGCTAGATCTCTTGCAATTGTTTGGGGTGATACTAAGCACTATTGGAACTGGATTCCTTTGCCTGATTCCAGGTTCCCTGAAGTTGCTAAGCTTGTTGACAACTGTCCTGTGGAGTTATCAGTTGGTGTCGAAGGTGGCCAGAGTAGTACTAAAATTGTCTGTTTGGATCCAAATGTGAAGTGGAAGTTCGGCATAGAAAATGAAGAAGTCCTGATGAGTTTGTTGGAGATAAAGGCTCTTAATTGGAAGAGTGGTCTCTTTCTTGAAGGAATAGAAATTAGGCCTAAGTAA
Protein sequence

>MsG0180004417.01.T01

MTEIQDLPEGCIAAIVSRTTPLDAGRLSVVSKTFRSASDSDAVWNQFLPSDISSIISHSPSLANAPTKKALYLALSDRPIIIDHDQKVFQLDRKSGKKCYMLSARSLAIVWGDTKHYWNWIPLPDSRFPEVAKLVDNCPVELSVGVEGGQSSTKIVCLDPNVKWKFGIENEEVLMSLLEIKALNWKSGLFLEGIEIRPK*