Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004770.01.T01 | XP_003591357.1 | 88.889 | 270 | 12 | 5 | 1 | 257 | 1 | 265 | 2.72E-153 | 437 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004110.01 | MsG0180004770.01 | 0.814911 | 1.210487e-51 | 8.226136e-49 |
| MsG0180004111.01 | MsG0180004770.01 | 0.807134 | 5.861893e-50 | 3.239241e-47 |
| MsG0180004770.01 | MsG0180004867.01 | 0.866753 | 2.263569e-65 | 7.715633e-62 |
| MsG0180004770.01 | MsG0180006047.01 | 0.820648 | 6.139258e-53 | 4.885583e-50 |
| MsG0180004770.01 | MsG0280006974.01 | 0.821116 | 4.791751e-53 | 3.863633e-50 |
| MsG0180004770.01 | MsG0280007073.01 | 0.808474 | 3.043064e-50 | 1.741632e-47 |
| MsG0180004770.01 | MsG0280007253.01 | 0.857500 | 1.564376e-62 | 3.869362e-59 |
| MsG0180004770.01 | MsG0380017550.01 | 0.801494 | 8.767334e-49 | 4.192965e-46 |
| MsG0180004770.01 | MsG0480022319.01 | 0.833201 | 6.110828e-56 | 6.987728e-53 |
| MsG0180004770.01 | MsG0480022895.01 | 0.804623 | 1.976779e-49 | 1.024063e-46 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004770.01.T01 | MTR_1g086510 | 88.889 | 270 | 12 | 5 | 1 | 257 | 1 | 265 | 3.30e-157 | 437 |
| MsG0180004770.01.T01 | MTR_1g086530 | 54.086 | 257 | 89 | 7 | 15 | 248 | 26 | 276 | 3.32e-83 | 250 |
| MsG0180004770.01.T01 | MTR_1g110460 | 54.011 | 187 | 72 | 2 | 8 | 182 | 14 | 198 | 3.21e-64 | 203 |
| MsG0180004770.01.T01 | MTR_2g033170 | 70.504 | 139 | 29 | 1 | 17 | 143 | 19 | 157 | 4.11e-62 | 198 |
| MsG0180004770.01.T01 | MTR_4g123040 | 68.310 | 142 | 33 | 1 | 14 | 143 | 16 | 157 | 7.26e-62 | 197 |
| MsG0180004770.01.T01 | MTR_7g035075 | 69.159 | 107 | 21 | 1 | 22 | 116 | 14 | 120 | 9.38e-50 | 162 |
| MsG0180004770.01.T01 | MTR_6g006030 | 67.593 | 108 | 23 | 1 | 22 | 117 | 14 | 121 | 2.91e-49 | 161 |
| MsG0180004770.01.T01 | MTR_1g045610 | 58.197 | 122 | 38 | 2 | 6 | 115 | 6 | 126 | 3.34e-43 | 147 |
| MsG0180004770.01.T01 | MTR_1g073170 | 61.905 | 105 | 28 | 1 | 23 | 115 | 21 | 125 | 1.12e-41 | 143 |
| MsG0180004770.01.T01 | MTR_5g029840 | 45.198 | 177 | 74 | 4 | 12 | 167 | 2 | 176 | 7.11e-40 | 141 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004770.01.T01 | AT2G47190 | 45.848 | 277 | 99 | 10 | 5 | 246 | 3 | 263 | 7.19e-69 | 214 |
| MsG0180004770.01.T01 | AT1G48000 | 67.361 | 144 | 35 | 1 | 22 | 153 | 32 | 175 | 3.85e-68 | 211 |
| MsG0180004770.01.T01 | AT5G49620 | 64.103 | 156 | 44 | 1 | 2 | 145 | 6 | 161 | 2.29e-62 | 198 |
| MsG0180004770.01.T01 | AT3G06490 | 66.667 | 150 | 34 | 2 | 10 | 143 | 3 | 152 | 9.38e-61 | 194 |
| MsG0180004770.01.T01 | AT1G68320 | 64.925 | 134 | 35 | 1 | 22 | 143 | 19 | 152 | 1.23e-59 | 191 |
| MsG0180004770.01.T01 | AT5G49620 | 58.824 | 170 | 44 | 2 | 2 | 145 | 6 | 175 | 1.09e-58 | 189 |
| MsG0180004770.01.T01 | AT1G25340 | 46.637 | 223 | 84 | 6 | 18 | 211 | 12 | 228 | 4.08e-58 | 186 |
| MsG0180004770.01.T01 | AT1G25340 | 63.704 | 135 | 40 | 2 | 18 | 143 | 12 | 146 | 1.38e-57 | 185 |
| MsG0180004770.01.T01 | AT1G25340 | 47.706 | 218 | 84 | 6 | 18 | 211 | 12 | 223 | 4.21e-57 | 185 |
| MsG0180004770.01.T01 | AT3G27810 | 66.364 | 110 | 25 | 1 | 19 | 116 | 17 | 126 | 1.39e-49 | 163 |
Find 62 sgRNAs with CRISPR-Local
Find 81 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATTGCACAACATCTTCC+GGG | 0.442714 | 1:+82507842 | MsG0180004770.01.T01:CDS |
| AACAACCAGGTTTCAGAACA+GGG | 0.521106 | 1:+82508211 | MsG0180004770.01.T01:CDS |
| AAGAGTACTGGTGCTTGTGA+TGG | 0.605785 | 1:+82508235 | MsG0180004770.01.T01:CDS |
| AAGCACATATATGAGAGGTA+TGG | 0.571829 | 1:+82507209 | None:intergenic |
| AATGAGACAGGGTTAAGAAA+AGG | 0.510276 | 1:+82507270 | MsG0180004770.01.T01:CDS |
| ACAACCAGGTTTCAGAACAG+GGG | 0.690824 | 1:+82508212 | MsG0180004770.01.T01:CDS |
| ACATTGCAAAGTTCAGTTTC+AGG | 0.345402 | 1:+82508070 | MsG0180004770.01.T01:CDS |
| ACCTATTGCCCCAGCGAGAG+TGG | 0.529174 | 1:-82507615 | None:intergenic |
| AGAAGGATATTTGATCCGTT+TGG | 0.402041 | 1:-82508037 | None:intergenic |
| AGGAATCAGTGGTGTTTCCT+CGG | 0.499809 | 1:+82508090 | MsG0180004770.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATTATTAAGGTTCAGAAAGG+TGG | + | Chr1:82507234-82507253 | MsG0180004770.01.T01:CDS | 30.0% | |
| ATTCCAAGTTGCTTCAAATT+CGG | + | Chr1:82508129-82508148 | MsG0180004770.01.T01:CDS | 30.0% | |
| TGTAATGTAATCAACCAGAA+TGG | - | Chr1:82507319-82507338 | None:intergenic | 30.0% | |
| TTATTAAGGTTCAGAAAGGT+GGG | + | Chr1:82507235-82507254 | MsG0180004770.01.T01:CDS | 30.0% | |
| AAAATTGCACAACATCTTCC+GGG | + | Chr1:82507842-82507861 | MsG0180004770.01.T01:CDS | 35.0% | |
| AATGAGACAGGGTTAAGAAA+AGG | + | Chr1:82507270-82507289 | MsG0180004770.01.T01:CDS | 35.0% | |
| ACATTGCAAAGTTCAGTTTC+AGG | + | Chr1:82508070-82508089 | MsG0180004770.01.T01:CDS | 35.0% | |
| AGAAGGATATTTGATCCGTT+TGG | - | Chr1:82508040-82508059 | None:intergenic | 35.0% | |
| ATTTCATTGTCTGTTCTTCC+CGG | - | Chr1:82507863-82507882 | None:intergenic | 35.0% | |
| GAAAATTGCACAACATCTTC+CGG | + | Chr1:82507841-82507860 | MsG0180004770.01.T01:CDS | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 82507219 | 82508351 | 82507219 | ID=MsG0180004770.01;Name=MsG0180004770.01 |
| Chr1 | mRNA | 82507219 | 82508351 | 82507219 | ID=MsG0180004770.01.T01;Parent=MsG0180004770.01;Name=MsG0180004770.01.T01;_AED=0.29;_eAED=0.32;_QI=0|0|0|1|1|1|3|0|257 |
| Chr1 | exon | 82507219 | 82507345 | 82507219 | ID=MsG0180004770.01.T01:exon:27287;Parent=MsG0180004770.01.T01 |
| Chr1 | exon | 82507506 | 82507635 | 82507506 | ID=MsG0180004770.01.T01:exon:27288;Parent=MsG0180004770.01.T01 |
| Chr1 | exon | 82507835 | 82508351 | 82507835 | ID=MsG0180004770.01.T01:exon:27289;Parent=MsG0180004770.01.T01 |
| Chr1 | CDS | 82507219 | 82507345 | 82507219 | ID=MsG0180004770.01.T01:cds;Parent=MsG0180004770.01.T01 |
| Chr1 | CDS | 82507506 | 82507635 | 82507506 | ID=MsG0180004770.01.T01:cds;Parent=MsG0180004770.01.T01 |
| Chr1 | CDS | 82507835 | 82508351 | 82507835 | ID=MsG0180004770.01.T01:cds;Parent=MsG0180004770.01.T01 |
| Gene Sequence |
>MsG0180004770.01.T01 ATGAGAGGTATGGATATTATTAAGGTTCAGAAAGGTGGGTCTGCAAAGGAAAATGAGACAGGGTTAAGAAAAGGTCCTTGGACATTAGAAGAGGACACCATTCTGGTTGATTACATTACAATACACGGTTTGAGGAGAAGTGGTAAAAGTTGCAGATTAAGGTGGCTAAACTACTTGCGTCCCGATGTACGCCGCGGGAATATCACAGTTCAAGAACAGATATTGATTCTTGACCTCCACTCTCGCTGGGGCAATAGGTGGTCGAAAATTGCACAACATCTTCCGGGAAGAACAGACAATGAAATAAAAAACTATTGGAGAACAAGAGTGATCAAGCAAGCAAAACAGCTCAAGTGTGATGTCAACAGCAAACAATTCAGAGACGTTTTACGTCACGTTTGGATGCCCCGATTGCTCGAACAAATTCAGCCCGCACAACAATTCCCCGACACGAATAATCCAAACGGATCAAATATCCTTCTTCAACAAAACACATTGCAAAGTTCAGTTTCAGGAATCAGTGGTGTTTCCTCGGACTCTTCATCAGTAGAATTCCAAGTTGCTTCAAATTCGGACAAAAATAATTCTTTGGAGCTTTTAGGCCATGAAGGTTCAAAACCATGGTCAAGTTTCAACAACCAGGTTTCAGAACAGGGGAAGAGTACTGGTGCTTGTGATGGTGACTCGTTGGAGAGTATGTGGAACGATGAGAACATGTGGTTTTTGCAACAACTTTACGAAGATGTTGAAATAAAATACAATTTACTTGCGTGA |
| Protein sequence |
>MsG0180004770.01.T01 MRGMDIIKVQKGGSAKENETGLRKGPWTLEEDTILVDYITIHGLRRSGKSCRLRWLNYLRPDVRRGNITVQEQILILDLHSRWGNRWSKIAQHLPGRTDNEIKNYWRTRVIKQAKQLKCDVNSKQFRDVLRHVWMPRLLEQIQPAQQFPDTNNPNGSNILLQQNTLQSSVSGISGVSSDSSSVEFQVASNSDKNNSLELLGHEGSKPWSSFNNQVSEQGKSTGACDGDSLESMWNDENMWFLQQLYEDVEIKYNLLA* |
