Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007253.01.T01 | XP_013462834.1 | 99.153 | 118 | 1 | 0 | 1 | 118 | 1 | 118 | 2.78E-80 | 241 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007253.01.T01 | M1C146 | 85.593 | 118 | 17 | 0 | 1 | 118 | 1 | 118 | 1.22E-73 | 217 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007253.01.T01 | I3S7J4 | 99.153 | 118 | 1 | 0 | 1 | 118 | 1 | 118 | 1.33e-80 | 241 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048964.01 | MsG0280007253.01 | 0.811773 | 5.920327e-51 | 3.697227e-48 |
| MsG0180000293.01 | MsG0280007253.01 | 0.812146 | 4.910495e-51 | 3.097712e-48 |
| MsG0180000503.01 | MsG0280007253.01 | 0.811173 | 7.990578e-51 | 4.911719e-48 |
| MsG0180000645.01 | MsG0280007253.01 | 0.855826 | 4.860377e-62 | 1.136685e-58 |
| MsG0180001551.01 | MsG0280007253.01 | 0.810114 | 1.353756e-50 | 8.091402e-48 |
| MsG0180002491.01 | MsG0280007253.01 | 0.803910 | 2.781965e-49 | 1.415150e-46 |
| MsG0180002912.01 | MsG0280007253.01 | 0.864577 | 1.100009e-64 | 3.470646e-61 |
| MsG0180003864.01 | MsG0280007253.01 | 0.806371 | 8.497417e-50 | 4.604268e-47 |
| MsG0180003948.01 | MsG0280007253.01 | 0.824544 | 7.621812e-54 | 6.772004e-51 |
| MsG0180004005.01 | MsG0280007253.01 | 0.806419 | 8.302274e-50 | 4.503956e-47 |
| MsG0180004079.01 | MsG0280007253.01 | 0.804210 | 2.409794e-49 | 1.235203e-46 |
| MsG0180004110.01 | MsG0280007253.01 | 0.870121 | 1.853838e-66 | 7.128902e-63 |
| MsG0180004111.01 | MsG0280007253.01 | 0.859474 | 4.035471e-63 | 1.067082e-59 |
| MsG0180004651.01 | MsG0280007253.01 | 0.835272 | 1.848275e-56 | 2.249166e-53 |
| MsG0180004770.01 | MsG0280007253.01 | 0.857500 | 1.564376e-62 | 3.869362e-59 |
| MsG0180004867.01 | MsG0280007253.01 | 0.834432 | 3.008003e-56 | 3.569088e-53 |
| MsG0180005331.01 | MsG0280007253.01 | 0.820609 | 6.265447e-53 | 4.980739e-50 |
| MsG0180005813.01 | MsG0280007253.01 | 0.866108 | 3.626896e-65 | 1.208268e-61 |
| MsG0180005842.01 | MsG0280007253.01 | 0.817362 | 3.429456e-52 | 2.492347e-49 |
| MsG0180005878.01 | MsG0280007253.01 | 0.817660 | 2.938013e-52 | 2.152705e-49 |
| MsG0180006012.01 | MsG0280007253.01 | 0.807327 | 5.336054e-50 | 2.963594e-47 |
| MsG0180006016.01 | MsG0280007253.01 | 0.863989 | 1.678891e-64 | 5.188383e-61 |
| MsG0180006047.01 | MsG0280007253.01 | 0.849743 | 2.658152e-60 | 5.089332e-57 |
| MsG0180006069.01 | MsG0280007253.01 | 0.844889 | 5.715155e-59 | 9.358158e-56 |
| MsG0280006356.01 | MsG0280007253.01 | 0.812092 | 5.045863e-51 | 3.178329e-48 |
| MsG0280006544.01 | MsG0280007253.01 | 0.827239 | 1.746011e-54 | 1.675928e-51 |
| MsG0280006595.01 | MsG0280007253.01 | 0.843956 | 1.018529e-58 | 1.619710e-55 |
| MsG0280006974.01 | MsG0280007253.01 | 0.867556 | 1.254520e-65 | 4.398644e-62 |
| MsG0280007073.01 | MsG0280007253.01 | 0.865894 | 4.239079e-65 | 1.401674e-61 |
| MsG0280007117.01 | MsG0280007253.01 | 0.820864 | 5.476138e-53 | 4.384756e-50 |
| MsG0280007253.01 | MsG0280007549.01 | 0.815192 | 1.048343e-51 | 7.178812e-49 |
| MsG0280007253.01 | MsG0280007564.01 | 0.805302 | 1.425552e-49 | 7.515430e-47 |
| MsG0280007253.01 | MsG0280007585.01 | 0.812016 | 5.240930e-51 | 3.294374e-48 |
| MsG0280007253.01 | MsG0280007648.01 | 0.814045 | 1.881518e-51 | 1.249087e-48 |
| MsG0280007253.01 | MsG0280007815.01 | 0.828525 | 8.565886e-55 | 8.535934e-52 |
| MsG0280007253.01 | MsG0280007957.01 | 0.802114 | 6.540986e-49 | 3.177690e-46 |
| MsG0280007253.01 | MsG0280008556.01 | 0.842055 | 3.265514e-58 | 4.891593e-55 |
| MsG0280007253.01 | MsG0280008856.01 | 0.813637 | 2.314313e-51 | 1.519497e-48 |
| MsG0280007253.01 | MsG0280009024.01 | 0.807983 | 3.871325e-50 | 2.187116e-47 |
| MsG0280007253.01 | MsG0280009235.01 | 0.822244 | 2.627633e-53 | 2.187150e-50 |
| MsG0280007253.01 | MsG0280009858.01 | 0.810384 | 1.184174e-50 | 7.127446e-48 |
| MsG0280007253.01 | MsG0280010416.01 | 0.825473 | 4.598194e-54 | 4.195156e-51 |
| MsG0280007253.01 | MsG0280011446.01 | 0.853951 | 1.701506e-61 | 3.739809e-58 |
| MsG0280007253.01 | MsG0280011457.01 | 0.839783 | 1.288641e-57 | 1.798498e-54 |
| MsG0280007253.01 | MsG0380012054.01 | 0.855390 | 6.513321e-62 | 1.501529e-58 |
| MsG0280007253.01 | MsG0380014365.01 | 0.802180 | 6.341193e-49 | 3.085751e-46 |
| MsG0280007253.01 | MsG0380014561.01 | 0.834351 | 3.151453e-56 | 3.730175e-53 |
| MsG0280007253.01 | MsG0380015181.01 | 0.853419 | 2.419200e-61 | 5.223977e-58 |
| MsG0280007253.01 | MsG0380016264.01 | 0.831109 | 2.010633e-55 | 2.160610e-52 |
| MsG0280007253.01 | MsG0380016367.01 | -0.819530 | 1.106679e-52 | 8.538505e-50 |
| MsG0280007253.01 | MsG0380016581.01 | 0.812839 | 3.464847e-51 | 2.226209e-48 |
| MsG0280007253.01 | MsG0380017346.01 | 0.842554 | 2.409645e-58 | 3.666891e-55 |
| MsG0280007253.01 | MsG0380017403.01 | 0.840520 | 8.276138e-58 | 1.181896e-54 |
| MsG0280007253.01 | MsG0380018013.01 | 0.813186 | 2.907466e-51 | 1.885586e-48 |
| MsG0280007253.01 | MsG0480018160.01 | 0.836577 | 8.627150e-57 | 1.091657e-53 |
| MsG0280007253.01 | MsG0480018333.01 | 0.833719 | 4.537678e-56 | 5.269472e-53 |
| MsG0280007253.01 | MsG0480018998.01 | 0.811717 | 6.087003e-51 | 3.795730e-48 |
| MsG0280007253.01 | MsG0480019816.01 | 0.824876 | 6.363519e-54 | 5.708582e-51 |
| MsG0280007253.01 | MsG0480019880.01 | 0.803939 | 2.743336e-49 | 1.396570e-46 |
| MsG0280007253.01 | MsG0480020090.01 | 0.824820 | 6.562450e-54 | 5.877384e-51 |
| MsG0280007253.01 | MsG0480020445.01 | 0.821271 | 4.413110e-53 | 3.573488e-50 |
| MsG0280007253.01 | MsG0480020886.01 | 0.813175 | 2.924168e-51 | 1.895819e-48 |
| MsG0280007253.01 | MsG0480021240.01 | 0.812192 | 4.796892e-51 | 3.029911e-48 |
| MsG0280007253.01 | MsG0480021298.01 | 0.840131 | 1.045985e-57 | 1.475898e-54 |
| MsG0280007253.01 | MsG0480022152.01 | 0.854992 | 8.502525e-62 | 1.934074e-58 |
| MsG0280007253.01 | MsG0480022274.01 | 0.831663 | 1.469673e-55 | 1.605571e-52 |
| MsG0280007253.01 | MsG0480022420.01 | 0.813953 | 1.971451e-51 | 1.305510e-48 |
| MsG0280007253.01 | MsG0480022480.01 | 0.812885 | 3.384691e-51 | 2.177494e-48 |
| MsG0280007253.01 | MsG0480023088.01 | 0.882723 | 8.199371e-71 | 5.049971e-67 |
| MsG0280007253.01 | MsG0480023224.01 | 0.841912 | 3.563726e-58 | 5.314263e-55 |
| MsG0280007253.01 | MsG0480023318.01 | 0.843382 | 1.450039e-58 | 2.264975e-55 |
| MsG0280007253.01 | MsG0480023517.01 | 0.807191 | 5.702867e-50 | 3.156033e-47 |
| MsG0280007253.01 | MsG0480023947.01 | 0.856605 | 2.874425e-62 | 6.899022e-59 |
| MsG0280007253.01 | MsG0580024137.01 | 0.811204 | 7.870875e-51 | 4.842018e-48 |
| MsG0280007253.01 | MsG0580024204.01 | 0.816765 | 4.670547e-52 | 3.339271e-49 |
| MsG0280007253.01 | MsG0580024334.01 | 0.811450 | 6.957362e-51 | 4.308130e-48 |
| MsG0280007253.01 | MsG0580024407.01 | 0.895036 | 1.403471e-75 | 1.431536e-71 |
| MsG0280007253.01 | MsG0580024575.01 | 0.867732 | 1.101777e-65 | 3.886703e-62 |
| MsG0280007253.01 | MsG0580024938.01 | 0.817980 | 2.488791e-52 | 1.839512e-49 |
| MsG0280007253.01 | MsG0580025115.01 | 0.802377 | 5.773838e-49 | 2.824092e-46 |
| MsG0280007253.01 | MsG0580025134.01 | 0.826561 | 2.535052e-54 | 2.385719e-51 |
| MsG0280007253.01 | MsG0580025143.01 | 0.840079 | 1.078944e-57 | 1.520053e-54 |
| MsG0280007253.01 | MsG0580025489.01 | 0.882986 | 6.569948e-71 | 4.089652e-67 |
| MsG0280007253.01 | MsG0580025989.01 | 0.810671 | 1.026563e-50 | 6.226755e-48 |
| MsG0280007253.01 | MsG0580026047.01 | 0.846136 | 2.625183e-59 | 4.474005e-56 |
| MsG0280007253.01 | MsG0580027345.01 | 0.862796 | 3.931570e-64 | 1.166042e-60 |
| MsG0280007253.01 | MsG0580027881.01 | 0.856513 | 3.058914e-62 | 7.320188e-59 |
| MsG0280007253.01 | MsG0580028185.01 | 0.813016 | 3.168197e-51 | 2.045366e-48 |
| MsG0280007253.01 | MsG0680031046.01 | 0.818703 | 1.706660e-52 | 1.286830e-49 |
| MsG0280007253.01 | MsG0680031055.01 | 0.814450 | 1.531006e-51 | 1.027638e-48 |
| MsG0280007253.01 | MsG0680033971.01 | 0.824200 | 9.181567e-54 | 8.077638e-51 |
| MsG0280007253.01 | MsG0680034190.01 | 0.801783 | 7.650753e-49 | 3.685655e-46 |
| MsG0280007253.01 | MsG0680035777.01 | 0.834117 | 3.607864e-56 | 4.240458e-53 |
| MsG0280007253.01 | MsG0780036876.01 | 0.876837 | 1.015955e-68 | 4.999055e-65 |
| MsG0280007253.01 | MsG0780037109.01 | 0.806842 | 6.758634e-50 | 3.706741e-47 |
| MsG0280007253.01 | MsG0780037895.01 | 0.800719 | 1.263309e-48 | 5.924137e-46 |
| MsG0280007253.01 | MsG0780038135.01 | 0.801934 | 7.124056e-49 | 3.445115e-46 |
| MsG0280007253.01 | MsG0780038921.01 | 0.874723 | 5.404488e-68 | 2.458690e-64 |
| MsG0280007253.01 | MsG0780038925.01 | 0.861028 | 1.368165e-63 | 3.816536e-60 |
| MsG0280007253.01 | MsG0780039354.01 | -0.805627 | 1.218565e-49 | 6.478210e-47 |
| MsG0280007253.01 | MsG0780039985.01 | 0.810764 | 9.800099e-51 | 5.959144e-48 |
| MsG0280007253.01 | MsG0780040143.01 | 0.831765 | 1.386222e-55 | 1.519190e-52 |
| MsG0280007253.01 | MsG0780040360.01 | 0.832742 | 7.948149e-56 | 8.964605e-53 |
| MsG0280007253.01 | MsG0780040442.01 | 0.884446 | 1.904381e-71 | 1.256454e-67 |
| MsG0280007253.01 | MsG0780040713.01 | 0.849647 | 2.826886e-60 | 5.395239e-57 |
| MsG0280007253.01 | MsG0780040749.01 | 0.815923 | 7.208823e-52 | 5.036355e-49 |
| MsG0280007253.01 | MsG0780041357.01 | 0.856281 | 3.576031e-62 | 8.490053e-59 |
| MsG0280007253.01 | MsG0780041754.01 | 0.802458 | 5.557220e-49 | 2.723580e-46 |
| MsG0280007253.01 | MsG0880041985.01 | 0.819886 | 9.176997e-53 | 7.150844e-50 |
| MsG0280007253.01 | MsG0880042843.01 | 0.839895 | 1.205121e-57 | 1.687861e-54 |
| MsG0280007253.01 | MsG0880042957.01 | 0.847644 | 1.014773e-59 | 1.815668e-56 |
| MsG0280007253.01 | MsG0880042967.01 | 0.838161 | 3.389370e-57 | 4.501078e-54 |
| MsG0280007253.01 | MsG0880042972.01 | 0.808948 | 2.409829e-50 | 1.396414e-47 |
| MsG0280007253.01 | MsG0880043001.01 | 0.807817 | 4.199704e-50 | 2.362322e-47 |
| MsG0280007253.01 | MsG0880043082.01 | 0.820100 | 8.196529e-53 | 6.425856e-50 |
| MsG0280007253.01 | MsG0880043094.01 | 0.836706 | 7.995496e-57 | 1.015732e-53 |
| MsG0280007253.01 | MsG0880043262.01 | 0.807437 | 5.058086e-50 | 2.817142e-47 |
| MsG0280007253.01 | MsG0880043285.01 | 0.838391 | 2.957133e-57 | 3.955066e-54 |
| MsG0280007253.01 | MsG0880043745.01 | 0.866027 | 3.849888e-65 | 1.278921e-61 |
| MsG0280007253.01 | MsG0880045106.01 | 0.836109 | 1.134362e-56 | 1.415514e-53 |
| MsG0280007253.01 | MsG0880045290.01 | 0.838888 | 2.200639e-57 | 2.988118e-54 |
| MsG0280007253.01 | MsG0880046058.01 | 0.811846 | 5.706758e-51 | 3.570785e-48 |
| MsG0280007253.01 | MsG0880046077.01 | 0.876034 | 1.923803e-68 | 9.185852e-65 |
| MsG0280007253.01 | MsG0880046209.01 | 0.805769 | 1.137539e-49 | 6.069887e-47 |
| MsG0280007253.01 | MsG0880046317.01 | 0.817456 | 3.267675e-52 | 2.380923e-49 |
| MsG0280007253.01 | MsG0880046329.01 | 0.839308 | 1.712167e-57 | 2.355035e-54 |
| MsG0280007253.01 | MsG0880046467.01 | 0.800139 | 1.657958e-48 | 7.661636e-46 |
| MsG0280007253.01 | MsG0880046733.01 | 0.800633 | 1.315163e-48 | 6.154155e-46 |
| MsG0280007253.01 | MsG0880046753.01 | 0.852028 | 6.039356e-61 | 1.245562e-57 |
| MsG0280007253.01 | MsG0880047143.01 | 0.805290 | 1.433628e-49 | 7.555759e-47 |
| MsG0280007253.01 | MsG0880047148.01 | 0.818735 | 1.678351e-52 | 1.266626e-49 |
| MsG0280007253.01 | MsG0880047559.01 | 0.810990 | 8.758510e-51 | 5.358502e-48 |
| MsG0280007253.01 | MsG0880047590.01 | 0.810450 | 1.145528e-50 | 6.907180e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007253.01.T01 | MTR_2g023430 | 99.153 | 118 | 1 | 0 | 1 | 118 | 1 | 118 | 3.37e-84 | 241 |
| MsG0280007253.01.T01 | MTR_4g048510 | 80.342 | 117 | 23 | 0 | 1 | 117 | 1 | 117 | 4.61e-71 | 208 |
| MsG0280007253.01.T01 | MTR_4g101090 | 76.923 | 117 | 27 | 0 | 1 | 117 | 1 | 117 | 5.06e-66 | 195 |
| MsG0280007253.01.T01 | MTR_2g088230 | 71.795 | 117 | 21 | 1 | 1 | 117 | 1 | 105 | 4.44e-58 | 175 |
| MsG0280007253.01.T01 | MTR_4g037225 | 72.174 | 115 | 30 | 1 | 1 | 113 | 1 | 115 | 5.62e-55 | 167 |
| MsG0280007253.01.T01 | MTR_4g123760 | 54.867 | 113 | 51 | 0 | 5 | 117 | 6 | 118 | 5.09e-46 | 145 |
| MsG0280007253.01.T01 | MTR_1g086310 | 55.357 | 112 | 50 | 0 | 6 | 117 | 7 | 118 | 4.37e-42 | 135 |
| MsG0280007253.01.T01 | MTR_7g096540 | 54.839 | 62 | 28 | 0 | 56 | 117 | 1 | 62 | 5.09e-24 | 87.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007253.01.T01 | AT4G21980 | 83.898 | 118 | 19 | 0 | 1 | 118 | 16 | 133 | 3.69e-73 | 214 |
| MsG0280007253.01.T01 | AT4G21980 | 83.898 | 118 | 19 | 0 | 1 | 118 | 1 | 118 | 4.13e-73 | 213 |
| MsG0280007253.01.T01 | AT1G62040 | 82.051 | 117 | 21 | 0 | 1 | 117 | 1 | 117 | 1.21e-71 | 209 |
| MsG0280007253.01.T01 | AT4G04620 | 83.051 | 118 | 20 | 0 | 1 | 118 | 1 | 118 | 5.21e-71 | 208 |
| MsG0280007253.01.T01 | AT4G04620 | 83.051 | 118 | 20 | 0 | 1 | 118 | 1 | 118 | 5.21e-71 | 208 |
| MsG0280007253.01.T01 | AT4G04620 | 83.051 | 118 | 20 | 0 | 1 | 118 | 1 | 118 | 5.21e-71 | 208 |
| MsG0280007253.01.T01 | AT2G05630 | 78.632 | 117 | 25 | 0 | 1 | 117 | 1 | 117 | 6.74e-69 | 202 |
| MsG0280007253.01.T01 | AT4G16520 | 76.068 | 117 | 28 | 0 | 1 | 117 | 1 | 117 | 2.75e-65 | 194 |
| MsG0280007253.01.T01 | AT4G16520 | 76.068 | 117 | 28 | 0 | 1 | 117 | 1 | 117 | 2.75e-65 | 194 |
| MsG0280007253.01.T01 | AT3G60640 | 73.729 | 118 | 31 | 0 | 1 | 118 | 1 | 118 | 1.66e-64 | 191 |
| MsG0280007253.01.T01 | AT2G05630 | 80.189 | 106 | 21 | 0 | 12 | 117 | 56 | 161 | 9.45e-63 | 189 |
| MsG0280007253.01.T01 | AT2G45170 | 72.269 | 119 | 32 | 1 | 1 | 118 | 1 | 119 | 2.67e-62 | 186 |
| MsG0280007253.01.T01 | AT2G45170 | 72.269 | 119 | 32 | 1 | 1 | 118 | 1 | 119 | 2.67e-62 | 186 |
| MsG0280007253.01.T01 | AT1G62040 | 79.775 | 89 | 18 | 0 | 1 | 89 | 1 | 89 | 1.16e-49 | 153 |
| MsG0280007253.01.T01 | AT4G16520 | 78.409 | 88 | 19 | 0 | 30 | 117 | 30 | 117 | 1.31e-47 | 149 |
| MsG0280007253.01.T01 | AT3G15580 | 57.522 | 113 | 48 | 0 | 5 | 117 | 3 | 115 | 5.59e-45 | 142 |
| MsG0280007253.01.T01 | AT3G06420 | 53.982 | 113 | 52 | 0 | 5 | 117 | 7 | 119 | 1.03e-41 | 134 |
Find 41 sgRNAs with CRISPR-Local
Find 125 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAATCGATGAATTTAGATTT+TGG | 0.191005 | 2:+12608075 | MsG0280007253.01.T01:three_prime_UTR |
| GTTTATATTGAATTGAAATT+AGG | 0.197923 | 2:-12605677 | None:intergenic |
| TGATCGAATCGAAGTGAAAT+TGG | 0.243113 | 2:-12605723 | None:intergenic |
| CATCCTTATGTTCTTCATAA+AGG | 0.263042 | 2:-12607872 | None:intergenic |
| AATTGAAATTAGGGTTTGTT+TGG | 0.264691 | 2:-12605667 | None:intergenic |
| AATTAGGGTTTGTTTGGGTT+TGG | 0.273530 | 2:-12605661 | None:intergenic |
| TTTATATTGAATTGAAATTA+GGG | 0.282883 | 2:-12605676 | None:intergenic |
| ATTAGGGTTTGTTTGGGTTT+GGG | 0.308222 | 2:-12605660 | None:intergenic |
| ATCCTTATGTTCTTCATAAA+GGG | 0.337341 | 2:-12607871 | None:intergenic |
| TTCAAGACTCAACATCCTTT+CGG | 0.352308 | 2:+12605908 | MsG0280007253.01.T01:CDS |
| GATCGAATCGAAGTGAAATT+GGG | 0.396149 | 2:-12605722 | None:intergenic |
| ATTGAAATTAGGGTTTGTTT+GGG | 0.407112 | 2:-12605666 | None:intergenic |
| GATGAAAGACGAAAGAATAA+TGG | 0.410914 | 2:-12605747 | None:intergenic |
| AATCTTACAGGTACTCTATC+AGG | 0.411891 | 2:-12606551 | None:intergenic |
| GTGTTGTGTGCAGGTGATTG+TGG | 0.446452 | 2:+12606640 | MsG0280007253.01.T01:intron |
| TACAGTGGAGAGAACACCTT+TGG | 0.449519 | 2:+12607918 | MsG0280007253.01.T01:CDS |
| GTCCCAGCTGACTTGTCTGT+TGG | 0.460647 | 2:+12607667 | MsG0280007253.01.T01:CDS |
| GTTAATGCATGTTTGAATTG+TGG | 0.467963 | 2:+12608097 | MsG0280007253.01.T01:three_prime_UTR |
| TGCCCTTTATGAAGAACATA+AGG | 0.471436 | 2:+12607869 | MsG0280007253.01.T01:CDS |
| GAAAGGATGTTGAGTCTTGA+AGG | 0.484284 | 2:-12605906 | None:intergenic |
| GAAAACACTCTACCTCCAAC+TGG | 0.492327 | 2:+12607757 | MsG0280007253.01.T01:CDS |
| TGACCAACAGACAAGTCAGC+TGG | 0.519715 | 2:-12607670 | None:intergenic |
| GAGAGCTATATACAGAAAGA+AGG | 0.536911 | 2:-12607967 | None:intergenic |
| GCTGGATACTTACCAGTTGG+AGG | 0.558063 | 2:-12607769 | None:intergenic |
| AATTAAGCTCAGTGCAGAGA+AGG | 0.558355 | 2:+12607717 | MsG0280007253.01.T01:CDS |
| ATGTTTGAATTGTGGTTGCT+CGG | 0.570274 | 2:+12608105 | MsG0280007253.01.T01:three_prime_UTR |
| CAGGTGATTGTGGAGAAAGC+TGG | 0.570859 | 2:+12606650 | MsG0280007253.01.T01:intron |
| GTATATGGCAGAAAGAAGAC+AGG | 0.574538 | 2:+12606499 | MsG0280007253.01.T01:intron |
| TATCTCAAATTATGCCTAGT+GGG | 0.579404 | 2:-12607943 | None:intergenic |
| GAACACCTTTGGTTCCCACT+AGG | 0.580132 | 2:+12607929 | MsG0280007253.01.T01:CDS |
| TTATGCCTAGTGGGAACCAA+AGG | 0.589647 | 2:-12607934 | None:intergenic |
| ATTTGCCATGTATTTGACAA+TGG | 0.591172 | 2:-12608001 | None:intergenic |
| AAAGGTGTTCTCTCCACTGT+AGG | 0.593040 | 2:-12607916 | None:intergenic |
| GCAGTCCATTGTCAAATACA+TGG | 0.593173 | 2:+12607996 | MsG0280007253.01.T01:three_prime_UTR |
| TTATGAAGAACATAAGGATG+AGG | 0.600323 | 2:+12607875 | MsG0280007253.01.T01:CDS |
| GACCAACAGACAAGTCAGCT+GGG | 0.605256 | 2:-12607669 | None:intergenic |
| ATCGAATCGAAGTGAAATTG+GGG | 0.606503 | 2:-12605721 | None:intergenic |
| GAAGAACATAAGGATGAGGA+TGG | 0.616176 | 2:+12607879 | MsG0280007253.01.T01:CDS |
| TTATCTCAAATTATGCCTAG+TGG | 0.627502 | 2:-12607944 | None:intergenic |
| CAGACAAGTCAGCTGGGACA+AGG | 0.641093 | 2:-12607663 | None:intergenic |
| TTTCTTTACATGACCTACAG+TGG | 0.650715 | 2:+12607903 | MsG0280007253.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTATATTGAATTGAAATTA+GGG | - | Chr2:12605679-12605698 | None:intergenic | 10.0% |
| !! | ATTAATAATAAATTACCGAA+AGG | - | Chr2:12605926-12605945 | None:intergenic | 15.0% |
| !! | GTTTATATTGAATTGAAATT+AGG | - | Chr2:12605680-12605699 | None:intergenic | 15.0% |
| !! | TATAACAAAGAATAGAAAAT+TGG | - | Chr2:12606821-12606840 | None:intergenic | 15.0% |
| !! | AATCAGAATTTAACAGAAAA+AGG | + | Chr2:12607201-12607220 | MsG0280007253.01.T01:intron | 20.0% |
| !! | ACAGAAAAAGATTAGAAATT+TGG | - | Chr2:12605824-12605843 | None:intergenic | 20.0% |
| !!! | ATTTAGGGTTTTTTACTATA+CGG | + | Chr2:12606070-12606089 | MsG0280007253.01.T01:intron | 20.0% |
| !!! | TAATCGATGAATTTAGATTT+TGG | + | Chr2:12608075-12608094 | MsG0280007253.01.T01:three_prime_UTR | 20.0% |
| !!! | TAATGAAGTATTGCTAAATA+TGG | + | Chr2:12606616-12606635 | MsG0280007253.01.T01:intron | 20.0% |
| ! | AATTTCATCGAATAACTAAC+AGG | - | Chr2:12606117-12606136 | None:intergenic | 25.0% |
| ! | ACTGTGTGATGATTAAATTT+AGG | + | Chr2:12606054-12606073 | MsG0280007253.01.T01:intron | 25.0% |
| ! | ATCCTTATGTTCTTCATAAA+GGG | - | Chr2:12607874-12607893 | None:intergenic | 25.0% |
| ! | ATTCAAGTCCAATAAACAAA+GGG | - | Chr2:12606463-12606482 | None:intergenic | 25.0% |
| ! | CAGGACAAAATAATATTCTT+AGG | - | Chr2:12606757-12606776 | None:intergenic | 25.0% |
| ! | CTGTGTGATGATTAAATTTA+GGG | + | Chr2:12606055-12606074 | MsG0280007253.01.T01:intron | 25.0% |
| ! | GTTAATAATCACAACCAAAT+TGG | - | Chr2:12607816-12607835 | None:intergenic | 25.0% |
| ! | TATTCAAGTCCAATAAACAA+AGG | - | Chr2:12606464-12606483 | None:intergenic | 25.0% |
| ! | TGCTTATAATAGTGATCATA+GGG | + | Chr2:12606137-12606156 | MsG0280007253.01.T01:intron | 25.0% |
| ! | TGTTATATAAATCGAGTGTT+AGG | + | Chr2:12606834-12606853 | MsG0280007253.01.T01:intron | 25.0% |
| ! | TTCATCTGATTGAAATTGTT+GGG | + | Chr2:12606031-12606050 | MsG0280007253.01.T01:intron | 25.0% |
| ! | TTGCTTATAATAGTGATCAT+AGG | + | Chr2:12606136-12606155 | MsG0280007253.01.T01:intron | 25.0% |
| !! | AATTGAAATTAGGGTTTGTT+TGG | - | Chr2:12605670-12605689 | None:intergenic | 25.0% |
| !! | ACAGTGTTATGTTTTGTATA+TGG | + | Chr2:12606484-12606503 | MsG0280007253.01.T01:intron | 25.0% |
| !! | AGAATATCAATTTTGCATTC+TGG | - | Chr2:12607032-12607051 | None:intergenic | 25.0% |
| !! | ATTGAAATTAGGGTTTGTTT+GGG | - | Chr2:12605669-12605688 | None:intergenic | 25.0% |
| !! | ATTTTGTCCTGAATTTTGTT+CGG | + | Chr2:12606766-12606785 | MsG0280007253.01.T01:intron | 25.0% |
| !! | GATTTCTTGAAGATTTTGAA+AGG | + | Chr2:12606793-12606812 | MsG0280007253.01.T01:intron | 25.0% |
| !! | TCGATTTTTAATCATGTCTA+AGG | + | Chr2:12605880-12605899 | MsG0280007253.01.T01:exon | 25.0% |
| !! | TCTGTGTTTTGTAATATTGT+TGG | + | Chr2:12606959-12606978 | MsG0280007253.01.T01:intron | 25.0% |
| !! | TTTGAAAACCCTTTGTTTAT+TGG | + | Chr2:12606452-12606471 | MsG0280007253.01.T01:intron | 25.0% |
| !!! | AATCTTGTTTTTTGCCAATT+TGG | + | Chr2:12607799-12607818 | MsG0280007253.01.T01:intron | 25.0% |
| AATATTAAACCAAGTCTCTG+CGG | + | Chr2:12607249-12607268 | MsG0280007253.01.T01:intron | 30.0% | |
| AGAGGTAGTAAAGTATGATA+TGG | + | Chr2:12606871-12606890 | MsG0280007253.01.T01:intron | 30.0% | |
| ATTCTGTTGCATATTCGATT+TGG | + | Chr2:12607047-12607066 | MsG0280007253.01.T01:intron | 30.0% | |
| ATTGTTGGAAATATGAAGCA+TGG | + | Chr2:12606974-12606993 | MsG0280007253.01.T01:intron | 30.0% | |
| ATTTGCCATGTATTTGACAA+TGG | - | Chr2:12608004-12608023 | None:intergenic | 30.0% | |
| CATAGTGAGTATAATCTTAC+AGG | - | Chr2:12606566-12606585 | None:intergenic | 30.0% | |
| CATCCTTATGTTCTTCATAA+AGG | - | Chr2:12607875-12607894 | None:intergenic | 30.0% | |
| CTTCATCTGATTGAAATTGT+TGG | + | Chr2:12606030-12606049 | MsG0280007253.01.T01:intron | 30.0% | |
| GATGAAAGACGAAAGAATAA+TGG | - | Chr2:12605750-12605769 | None:intergenic | 30.0% | |
| GCAAAAAACAAGATTTCGTA+TGG | - | Chr2:12607794-12607813 | None:intergenic | 30.0% | |
| GTTAATGCATGTTTGAATTG+TGG | + | Chr2:12608097-12608116 | MsG0280007253.01.T01:three_prime_UTR | 30.0% | |
| TATCTCAAATTATGCCTAGT+GGG | - | Chr2:12607946-12607965 | None:intergenic | 30.0% | |
| TTATCTCAAATTATGCCTAG+TGG | - | Chr2:12607947-12607966 | None:intergenic | 30.0% | |
| TTATGAAGAACATAAGGATG+AGG | + | Chr2:12607875-12607894 | MsG0280007253.01.T01:CDS | 30.0% | |
| TTCTTCAGATTTGTCCATAT+CGG | + | Chr2:12607355-12607374 | MsG0280007253.01.T01:intron | 30.0% | |
| ! | GTTGGTCAATTTGTTCTAAT+TGG | + | Chr2:12607613-12607632 | MsG0280007253.01.T01:intron | 30.0% |
| !! | TATTCTTTGTGTGTTGCTTT+AGG | + | Chr2:12607566-12607585 | MsG0280007253.01.T01:intron | 30.0% |
| AAAACAAGATTTCGTATGGC+TGG | - | Chr2:12607790-12607809 | None:intergenic | 35.0% | |
| AAACCAGTATCAACTCAACA+AGG | - | Chr2:12606902-12606921 | None:intergenic | 35.0% | |
| AATCTTACAGGTACTCTATC+AGG | - | Chr2:12606554-12606573 | None:intergenic | 35.0% | |
| AGTCCAAAGATGTAAAGATG+TGG | - | Chr2:12607146-12607165 | None:intergenic | 35.0% | |
| ATCAAGACCGAACAAAATTC+AGG | - | Chr2:12606776-12606795 | None:intergenic | 35.0% | |
| ATCGAATCGAAGTGAAATTG+GGG | - | Chr2:12605724-12605743 | None:intergenic | 35.0% | |
| ATTCTTTCGTCTTTCATCGT+GGG | + | Chr2:12605752-12605771 | MsG0280007253.01.T01:intron | 35.0% | |
| GAAAATCAGATGATCAAGCT+TGG | + | Chr2:12607461-12607480 | MsG0280007253.01.T01:intron | 35.0% | |
| GAATACTGCATATGCATATG+TGG | + | Chr2:12607300-12607319 | MsG0280007253.01.T01:intron | 35.0% | |
| GAGAGCTATATACAGAAAGA+AGG | - | Chr2:12607970-12607989 | None:intergenic | 35.0% | |
| GATCGAATCGAAGTGAAATT+GGG | - | Chr2:12605725-12605744 | None:intergenic | 35.0% | |
| GTTAAACTAGAGTCTCATCT+GGG | - | Chr2:12607394-12607413 | None:intergenic | 35.0% | |
| TATTCTTTCGTCTTTCATCG+TGG | + | Chr2:12605751-12605770 | MsG0280007253.01.T01:intron | 35.0% | |
| TGATCGAATCGAAGTGAAAT+TGG | - | Chr2:12605726-12605745 | None:intergenic | 35.0% | |
| TGCCCTTTATGAAGAACATA+AGG | + | Chr2:12607869-12607888 | MsG0280007253.01.T01:CDS | 35.0% | |
| TGTTAAACTAGAGTCTCATC+TGG | - | Chr2:12607395-12607414 | None:intergenic | 35.0% | |
| TTAAACTAGAGTCTCATCTG+GGG | - | Chr2:12607393-12607412 | None:intergenic | 35.0% | |
| TTAACATTCAAGAGATGCTC+CGG | - | Chr2:12607423-12607442 | None:intergenic | 35.0% | |
| TTCAAGACTCAACATCCTTT+CGG | + | Chr2:12605908-12605927 | MsG0280007253.01.T01:CDS | 35.0% | |
| TTTCTTTACATGACCTACAG+TGG | + | Chr2:12607903-12607922 | MsG0280007253.01.T01:CDS | 35.0% | |
| ! | AATTAGGGTTTGTTTGGGTT+TGG | - | Chr2:12605664-12605683 | None:intergenic | 35.0% |
| ! | ACTCTAGTTTAACATCTTCC+CGG | + | Chr2:12607401-12607420 | MsG0280007253.01.T01:intron | 35.0% |
| ! | ATTAGGGTTTGTTTGGGTTT+GGG | - | Chr2:12605663-12605682 | None:intergenic | 35.0% |
| ! | CACTGAGCTTAATTCTTTTG+CGG | - | Chr2:12607711-12607730 | None:intergenic | 35.0% |
| ! | CACTGCAAATTTTCATGCTT+TGG | + | Chr2:12607527-12607546 | MsG0280007253.01.T01:intron | 35.0% |
| ! | TGCAAATTTTCATGCTTTGG+TGG | + | Chr2:12607530-12607549 | MsG0280007253.01.T01:intron | 35.0% |
| !! | ATATTCGATTTGGCCAAGTA+TGG | + | Chr2:12607057-12607076 | MsG0280007253.01.T01:intron | 35.0% |
| !! | ATGCTTTGGTGGATTCTAAT+TGG | + | Chr2:12607541-12607560 | MsG0280007253.01.T01:intron | 35.0% |
| !! | CTGGTAATTTGGTTTGAGAA+GGG | + | Chr2:12607278-12607297 | MsG0280007253.01.T01:intron | 35.0% |
| !! | TATTCGATTTGGCCAAGTAT+GGG | + | Chr2:12607058-12607077 | MsG0280007253.01.T01:intron | 35.0% |
| !! | TCTGGTAATTTGGTTTGAGA+AGG | + | Chr2:12607277-12607296 | MsG0280007253.01.T01:intron | 35.0% |
| !! | TGCGGATATTTCTGGTAATT+TGG | + | Chr2:12607267-12607286 | MsG0280007253.01.T01:intron | 35.0% |
| AAATATGGTGTGTTGTGTGC+AGG | + | Chr2:12606631-12606650 | MsG0280007253.01.T01:intron | 40.0% | |
| AATTAAGCTCAGTGCAGAGA+AGG | + | Chr2:12607717-12607736 | MsG0280007253.01.T01:CDS | 40.0% | |
| AGTGACCAGCAATTTAAGGA+TGG | - | Chr2:12607098-12607117 | None:intergenic | 40.0% | |
| ATAATACACTGCCCCATACT+TGG | - | Chr2:12607073-12607092 | None:intergenic | 40.0% | |
| ATCGTGTTAGATTTGTCCTC+TGG | + | Chr2:12607118-12607137 | MsG0280007253.01.T01:intron | 40.0% | |
| CGATAGTGACCAGCAATTTA+AGG | - | Chr2:12607102-12607121 | None:intergenic | 40.0% | |
| GAAAGGATGTTGAGTCTTGA+AGG | - | Chr2:12605909-12605928 | None:intergenic | 40.0% | |
| GAAGAACATAAGGATGAGGA+TGG | + | Chr2:12607879-12607898 | MsG0280007253.01.T01:CDS | 40.0% | |
| GCACCACATCTTTACATCTT+TGG | + | Chr2:12607140-12607159 | MsG0280007253.01.T01:intron | 40.0% | |
| GCAGTCCATTGTCAAATACA+TGG | + | Chr2:12607996-12608015 | MsG0280007253.01.T01:three_prime_UTR | 40.0% | |
| GGTTAAGTTAACTGCCGATA+TGG | - | Chr2:12607372-12607391 | None:intergenic | 40.0% | |
| GTATATGGCAGAAAGAAGAC+AGG | + | Chr2:12606499-12606518 | MsG0280007253.01.T01:intron | 40.0% | |
| GTGACCAGCAATTTAAGGAT+GGG | - | Chr2:12607097-12607116 | None:intergenic | 40.0% | |
| GTGATTGATTGTTGCAGTGT+CGG | + | Chr2:12606299-12606318 | MsG0280007253.01.T01:intron | 40.0% | |
| TAACATTCAAGAGATGCTCC+GGG | - | Chr2:12607422-12607441 | None:intergenic | 40.0% | |
| TATGCCCATCCTTAAATTGC+TGG | + | Chr2:12607090-12607109 | MsG0280007253.01.T01:intron | 40.0% | |
| ! | ATTAGCGTTCTAAACACGTG+TGG | - | Chr2:12606412-12606431 | None:intergenic | 40.0% |
| !! | ATTCGATTTGGCCAAGTATG+GGG | + | Chr2:12607059-12607078 | MsG0280007253.01.T01:intron | 40.0% |
| !! | CATCCTTGTTGAGTTGATAC+TGG | + | Chr2:12606896-12606915 | MsG0280007253.01.T01:intron | 40.0% |
| ACCAACACCTCGAATTGAAG+AGG | - | Chr2:12606388-12606407 | None:intergenic | 45.0% | |
| ACCTCTTCAATTCGAGGTGT+TGG | + | Chr2:12606384-12606403 | MsG0280007253.01.T01:intron | 45.0% | |
| ATCAGAGCAGCTGTAACAAC+AGG | - | Chr2:12607846-12607865 | None:intergenic | 45.0% | |
| ATGGCTGGATACTTACCAGT+TGG | - | Chr2:12607775-12607794 | None:intergenic | 45.0% | |
| CAAGTCTCTGCGGATATTTC+TGG | + | Chr2:12607259-12607278 | MsG0280007253.01.T01:intron | 45.0% | |
| CAGAAATATCCGCAGAGACT+TGG | - | Chr2:12607261-12607280 | None:intergenic | 45.0% | |
| GAAAACACTCTACCTCCAAC+TGG | + | Chr2:12607757-12607776 | MsG0280007253.01.T01:CDS | 45.0% | |
| TACAGTGGAGAGAACACCTT+TGG | + | Chr2:12607918-12607937 | MsG0280007253.01.T01:CDS | 45.0% | |
| TAGGCTTGTAGCTAGTGAAG+AGG | + | Chr2:12606853-12606872 | MsG0280007253.01.T01:intron | 45.0% | |
| TCTGACACCTCTTCAATTCG+AGG | + | Chr2:12606378-12606397 | MsG0280007253.01.T01:intron | 45.0% | |
| TTATGCCTAGTGGGAACCAA+AGG | - | Chr2:12607937-12607956 | None:intergenic | 45.0% | |
| ! | ATGTAAAGATGTGGTGCCAG+AGG | - | Chr2:12607137-12607156 | None:intergenic | 45.0% |
| ! | TGCATTCTGGTGTCAGCTAT+TGG | - | Chr2:12607019-12607038 | None:intergenic | 45.0% |
| !! | AAAGGTGTTCTCTCCACTGT+AGG | - | Chr2:12607919-12607938 | None:intergenic | 45.0% |
| !! | AAAAATCTTAATAAAATATA+TGG | - | Chr2:12607175-12607194 | None:intergenic | 5.0% |
| AGAGCAGCTGTAACAACAGG+AGG | - | Chr2:12607843-12607862 | None:intergenic | 50.0% | |
| CAGGTGATTGTGGAGAAAGC+TGG | + | Chr2:12606650-12606669 | MsG0280007253.01.T01:intron | 50.0% | |
| GACCAACAGACAAGTCAGCT+GGG | - | Chr2:12607600-12607619 | None:intergenic | 50.0% | |
| GCTGGATACTTACCAGTTGG+AGG | - | Chr2:12607772-12607791 | None:intergenic | 50.0% | |
| GTGTCAGATGTCGACACATG+TGG | - | Chr2:12606366-12606385 | None:intergenic | 50.0% | |
| GTGTTGTGTGCAGGTGATTG+TGG | + | Chr2:12606640-12606659 | MsG0280007253.01.T01:intron | 50.0% | |
| TGACCAACAGACAAGTCAGC+TGG | - | Chr2:12607601-12607620 | None:intergenic | 50.0% | |
| ! | GAACACCTTTGGTTCCCACT+AGG | + | Chr2:12607929-12607948 | MsG0280007253.01.T01:CDS | 50.0% |
| ! | TCTGGTGTCAGCTATTGGAG+TGG | - | Chr2:12607014-12607033 | None:intergenic | 50.0% |
| CAGACAAGTCAGCTGGGACA+AGG | - | Chr2:12607594-12607613 | None:intergenic | 55.0% | |
| !! | GTCCCAGCTGACTTGTCTGT+TGG | + | Chr2:12607595-12607614 | MsG0280007253.01.T01:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 12605654 | 12608125 | 12605654 | ID=MsG0280007253.01;Name=MsG0280007253.01 |
| Chr2 | mRNA | 12605654 | 12608125 | 12605654 | ID=MsG0280007253.01.T01;Parent=MsG0280007253.01;Name=MsG0280007253.01.T01;_AED=0.38;_eAED=0.38;_QI=147|1|1|1|0.6|0.5|6|175|119 |
| Chr2 | exon | 12605654 | 12605769 | 12605654 | ID=MsG0280007253.01.T01:exon:3720;Parent=MsG0280007253.01.T01 |
| Chr2 | exon | 12605862 | 12605929 | 12605862 | ID=MsG0280007253.01.T01:exon:3721;Parent=MsG0280007253.01.T01 |
| Chr2 | exon | 12606510 | 12606565 | 12606510 | ID=MsG0280007253.01.T01:exon:3722;Parent=MsG0280007253.01.T01 |
| Chr2 | exon | 12606653 | 12606705 | 12606653 | ID=MsG0280007253.01.T01:exon:3723;Parent=MsG0280007253.01.T01 |
| Chr2 | exon | 12607660 | 12607778 | 12607660 | ID=MsG0280007253.01.T01:exon:3724;Parent=MsG0280007253.01.T01 |
| Chr2 | exon | 12607856 | 12608125 | 12607856 | ID=MsG0280007253.01.T01:exon:3725;Parent=MsG0280007253.01.T01 |
| Chr2 | five_prime_UTR | 12605654 | 12605769 | 12605654 | ID=MsG0280007253.01.T01:five_prime_utr;Parent=MsG0280007253.01.T01 |
| Chr2 | five_prime_UTR | 12605862 | 12605892 | 12605862 | ID=MsG0280007253.01.T01:five_prime_utr;Parent=MsG0280007253.01.T01 |
| Chr2 | CDS | 12605893 | 12605929 | 12605893 | ID=MsG0280007253.01.T01:cds;Parent=MsG0280007253.01.T01 |
| Chr2 | CDS | 12606510 | 12606565 | 12606510 | ID=MsG0280007253.01.T01:cds;Parent=MsG0280007253.01.T01 |
| Chr2 | CDS | 12606653 | 12606705 | 12606653 | ID=MsG0280007253.01.T01:cds;Parent=MsG0280007253.01.T01 |
| Chr2 | CDS | 12607660 | 12607778 | 12607660 | ID=MsG0280007253.01.T01:cds;Parent=MsG0280007253.01.T01 |
| Chr2 | CDS | 12607856 | 12607950 | 12607856 | ID=MsG0280007253.01.T01:cds;Parent=MsG0280007253.01.T01 |
| Chr2 | three_prime_UTR | 12607951 | 12608125 | 12607951 | ID=MsG0280007253.01.T01:three_prime_utr;Parent=MsG0280007253.01.T01 |
| Gene Sequence |
| Protein sequence |
