Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004111.01.T01 | XP_003590795.1 | 90 | 70 | 7 | 0 | 1 | 70 | 1 | 70 | 4.55E-38 | 131 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004111.01.T01 | P19873 | 60.294 | 68 | 27 | 0 | 3 | 70 | 1 | 68 | 1.55E-23 | 87 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004111.01.T01 | G7I3Z2 | 90.000 | 70 | 7 | 0 | 1 | 70 | 1 | 70 | 2.17e-38 | 131 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000337.01 | MsG0180004111.01 | 0.801443 | 8.983441e-49 | 4.290671e-46 |
| MsG0180004110.01 | MsG0180004111.01 | 0.920168 | 1.800766e-87 | 6.015775e-83 |
| MsG0180004111.01 | MsG0180004154.01 | 0.802565 | 5.283013e-49 | 2.596154e-46 |
| MsG0180004111.01 | MsG0180004770.01 | 0.807134 | 5.861893e-50 | 3.239241e-47 |
| MsG0180004111.01 | MsG0180004867.01 | 0.835815 | 1.346870e-56 | 1.665662e-53 |
| MsG0180004111.01 | MsG0280006475.01 | -0.813185 | 2.909384e-51 | 1.886760e-48 |
| MsG0180004111.01 | MsG0280006974.01 | 0.862477 | 4.929882e-64 | 1.445858e-60 |
| MsG0180004111.01 | MsG0280007253.01 | 0.859474 | 4.035471e-63 | 1.067082e-59 |
| MsG0180004111.01 | MsG0280007579.01 | 0.817954 | 2.522193e-52 | 1.862918e-49 |
| MsG0180004111.01 | MsG0280009858.01 | 0.833809 | 4.307992e-56 | 5.016953e-53 |
| MsG0180004111.01 | MsG0380012054.01 | 0.804730 | 1.877495e-49 | 9.754277e-47 |
| MsG0180004111.01 | MsG0480018333.01 | 0.849368 | 3.382389e-60 | 6.397983e-57 |
| MsG0180004111.01 | MsG0480019295.01 | 0.805009 | 1.641852e-49 | 8.590895e-47 |
| MsG0180004111.01 | MsG0480022152.01 | 0.807268 | 5.491398e-50 | 3.045221e-47 |
| MsG0180004111.01 | MsG0480022274.01 | 0.803729 | 3.033973e-49 | 1.536093e-46 |
| MsG0180004111.01 | MsG0480022530.01 | 0.810995 | 8.735573e-51 | 5.345166e-48 |
| MsG0180004111.01 | MsG0480022760.01 | 0.818817 | 1.608209e-52 | 1.216424e-49 |
| MsG0180004111.01 | MsG0480023318.01 | 0.845540 | 3.810416e-59 | 6.371020e-56 |
| MsG0180004111.01 | MsG0480023947.01 | 0.858991 | 5.633402e-63 | 1.465033e-59 |
| MsG0180004111.01 | MsG0580024407.01 | 0.841219 | 5.426035e-58 | 7.917917e-55 |
| MsG0180004111.01 | MsG0580024575.01 | 0.806553 | 7.778615e-50 | 4.234552e-47 |
| MsG0180004111.01 | MsG0580029583.01 | 0.858078 | 1.054478e-62 | 2.659016e-59 |
| MsG0180004111.01 | MsG0680031055.01 | 0.829190 | 5.911722e-55 | 6.007185e-52 |
| MsG0180004111.01 | MsG0780036876.01 | 0.822432 | 2.376709e-53 | 1.989066e-50 |
| MsG0180004111.01 | MsG0780038123.01 | 0.800256 | 1.569800e-48 | 7.275735e-46 |
| MsG0180004111.01 | MsG0780040144.01 | 0.812042 | 5.172290e-51 | 3.253364e-48 |
| MsG0180004111.01 | MsG0780041357.01 | 0.805099 | 1.571936e-49 | 8.244255e-47 |
| MsG0180004111.01 | MsG0880043094.01 | 0.825993 | 3.462505e-54 | 3.206411e-51 |
| MsG0180004111.01 | MsG0880043285.01 | 0.837320 | 5.572901e-57 | 7.212474e-54 |
| MsG0180004111.01 | MsG0880043744.01 | 0.820881 | 5.425637e-53 | 4.346368e-50 |
| MsG0180004111.01 | MsG0880043745.01 | 0.803035 | 4.223245e-49 | 2.100421e-46 |
| MsG0180004111.01 | MsG0880045277.01 | 0.812174 | 4.841629e-51 | 3.056589e-48 |
| MsG0180004111.01 | MsG0880045819.01 | 0.848090 | 7.647620e-60 | 1.387534e-56 |
| MsG0180004111.01 | MsG0880046753.01 | 0.802545 | 5.332299e-49 | 2.619158e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004111.01.T01 | MTR_1g075340 | 90.000 | 70 | 7 | 0 | 1 | 70 | 1 | 70 | 5.51e-42 | 131 |
| MsG0180004111.01.T01 | MTR_1g075340 | 90.000 | 70 | 7 | 0 | 1 | 70 | 1 | 70 | 5.51e-42 | 131 |
| MsG0180004111.01.T01 | MTR_1g075410 | 83.824 | 68 | 11 | 0 | 3 | 70 | 5 | 72 | 1.92e-36 | 117 |
| MsG0180004111.01.T01 | MTR_5g090250 | 84.286 | 70 | 11 | 0 | 1 | 70 | 1 | 70 | 2.85e-36 | 116 |
| MsG0180004111.01.T01 | MTR_5g090250 | 84.286 | 70 | 11 | 0 | 1 | 70 | 1 | 70 | 2.85e-36 | 116 |
| MsG0180004111.01.T01 | MTR_1g075380 | 79.412 | 68 | 14 | 0 | 3 | 70 | 4 | 71 | 8.80e-32 | 105 |
| MsG0180004111.01.T01 | MTR_1g070335 | 54.286 | 70 | 32 | 0 | 1 | 70 | 1 | 70 | 9.30e-22 | 80.1 |
| MsG0180004111.01.T01 | MTR_5g045470 | 53.731 | 67 | 31 | 0 | 4 | 70 | 3 | 69 | 7.04e-19 | 72.8 |
| MsG0180004111.01.T01 | MTR_1g070330 | 55.556 | 54 | 24 | 0 | 17 | 70 | 1 | 54 | 1.41e-15 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004111.01.T01 | AT2G38870 | 52.857 | 70 | 33 | 0 | 1 | 70 | 1 | 70 | 1.51e-21 | 79.7 |
| MsG0180004111.01.T01 | AT5G43580 | 55.556 | 72 | 30 | 1 | 1 | 70 | 28 | 99 | 5.05e-21 | 79.0 |
| MsG0180004111.01.T01 | AT3G46860 | 46.032 | 63 | 34 | 0 | 8 | 70 | 23 | 85 | 4.13e-13 | 58.5 |
| MsG0180004111.01.T01 | AT5G43570 | 46.575 | 73 | 33 | 1 | 1 | 67 | 1 | 73 | 6.26e-12 | 56.2 |
| MsG0180004111.01.T01 | AT2G38900 | 42.188 | 64 | 37 | 0 | 7 | 70 | 10 | 73 | 2.12e-11 | 53.9 |
| MsG0180004111.01.T01 | AT2G38900 | 42.188 | 64 | 37 | 0 | 7 | 70 | 25 | 88 | 3.22e-11 | 53.9 |
| MsG0180004111.01.T01 | AT5G43570 | 50.000 | 60 | 30 | 0 | 8 | 67 | 24 | 83 | 6.65e-11 | 53.9 |
Find 30 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTCCACTCCAACCAATTC+TGG | 0.174728 | 1:-73520499 | None:intergenic |
| CTGTGACAGGGTTTGGGTTT+GGG | 0.259063 | 1:+73520612 | MsG0180004111.01.T01:CDS |
| ACTTCCGCTGTGACAGGGTT+TGG | 0.267379 | 1:+73520605 | MsG0180004111.01.T01:CDS |
| CTTCCGCTGTGACAGGGTTT+GGG | 0.309008 | 1:+73520606 | MsG0180004111.01.T01:CDS |
| GCTGTGACAGGGTTTGGGTT+TGG | 0.326505 | 1:+73520611 | MsG0180004111.01.T01:CDS |
| TAAAGATGGAATTGTCTATC+AGG | 0.326953 | 1:+73520639 | MsG0180004111.01.T01:CDS |
| CCTTTGACTTCCGCTGTGAC+AGG | 0.333094 | 1:+73520599 | MsG0180004111.01.T01:CDS |
| ATAAATTTAATGTTACAATA+AGG | 0.340780 | 1:+73520719 | MsG0180004111.01.T01:three_prime_UTR |
| TAAGAGTTCATGGCCAGAAT+TGG | 0.414180 | 1:+73520486 | MsG0180004111.01.T01:CDS |
| ATAGTAGTATAGTATAGAAT+TGG | 0.416384 | 1:-73520757 | None:intergenic |
| TGGGTTTGGGTTAATAAAGA+TGG | 0.418377 | 1:+73520625 | MsG0180004111.01.T01:CDS |
| TGTGGTCAGGTAAGAGTTCA+TGG | 0.426967 | 1:+73520476 | MsG0180004111.01.T01:intron |
| GTAACATTAAATTTATAACT+TGG | 0.441318 | 1:-73520712 | None:intergenic |
| AAATTGGATAGAAAGTTTCA+TGG | 0.443912 | 1:+73520668 | MsG0180004111.01.T01:exon |
| CTTTGACTTCCGCTGTGACA+GGG | 0.463269 | 1:+73520600 | MsG0180004111.01.T01:CDS |
| CTGTCACAGCGGAAGTCAAA+GGG | 0.467050 | 1:-73520598 | None:intergenic |
| GATGCCATCATTGTGCTAGA+AGG | 0.504821 | 1:+73520565 | MsG0180004111.01.T01:CDS |
| CCAGAATTGGTTGGAGTGGA+AGG | 0.515293 | 1:+73520499 | MsG0180004111.01.T01:CDS |
| AGTTCCTTCTAGCACAATGA+TGG | 0.538007 | 1:-73520569 | None:intergenic |
| AATTCAGAGGGAGAATCCAT+TGG | 0.542280 | 1:+73520540 | MsG0180004111.01.T01:CDS |
| CCTGTCACAGCGGAAGTCAA+AGG | 0.545493 | 1:-73520599 | None:intergenic |
| ATGGCCAGAATTGGTTGGAG+TGG | 0.561764 | 1:+73520495 | MsG0180004111.01.T01:CDS |
| AGTTCATGGCCAGAATTGGT+TGG | 0.565712 | 1:+73520490 | MsG0180004111.01.T01:CDS |
| ATTGGTTGGAGTGGAAGGAA+AGG | 0.570071 | 1:+73520504 | MsG0180004111.01.T01:CDS |
| AGCAGAAGCTACAATTCAGA+GGG | 0.593359 | 1:+73520528 | MsG0180004111.01.T01:CDS |
| TAGCAGAAGCTACAATTCAG+AGG | 0.596029 | 1:+73520527 | MsG0180004111.01.T01:CDS |
| ACAATGATGGCATCAACCAA+TGG | 0.650325 | 1:-73520556 | None:intergenic |
| AAAATGTCTGATGAATGCAA+AGG | 0.675933 | 1:+73520322 | None:intergenic |
| TGTCACAGCGGAAGTCAAAG+GGG | 0.698463 | 1:-73520597 | None:intergenic |
| AAACCCAAACCCTGTCACAG+CGG | 0.730650 | 1:-73520609 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAATTTAATGTTACAATA+AGG | + | Chr1:73520719-73520738 | MsG0180004111.01.T01:three_prime_UTR | 10.0% |
| !! | TATATGAATAATAAATTGAA+TGG | + | Chr1:73520406-73520425 | MsG0180004111.01.T01:intron | 10.0% |
| !! | GTAACATTAAATTTATAACT+TGG | - | Chr1:73520715-73520734 | None:intergenic | 15.0% |
| !!! | TTACATGCATATATATTTTT+TGG | + | Chr1:73520369-73520388 | MsG0180004111.01.T01:intron | 15.0% |
| !! | ATAGTAGTATAGTATAGAAT+TGG | - | Chr1:73520760-73520779 | None:intergenic | 20.0% |
| ! | AAATTGGATAGAAAGTTTCA+TGG | + | Chr1:73520668-73520687 | MsG0180004111.01.T01:exon | 25.0% |
| ! | TTGAATGAATCAATATTGTG+TGG | + | Chr1:73520458-73520477 | MsG0180004111.01.T01:intron | 25.0% |
| !! | AACTTGTAATTCGTTTTCTT+TGG | + | Chr1:73520827-73520846 | MsG0180004111.01.T01:three_prime_UTR | 25.0% |
| !! | GAAACTTTCTATCCAATTTT+TGG | - | Chr1:73520667-73520686 | None:intergenic | 25.0% |
| TAAAGATGGAATTGTCTATC+AGG | + | Chr1:73520639-73520658 | MsG0180004111.01.T01:CDS | 30.0% | |
| GTCTATCAGGTTCCAAAAAT+TGG | + | Chr1:73520652-73520671 | MsG0180004111.01.T01:CDS | 35.0% | |
| TGAATCAATATTGTGTGGTC+AGG | + | Chr1:73520463-73520482 | MsG0180004111.01.T01:intron | 35.0% | |
| ! | TGGGTTTGGGTTAATAAAGA+TGG | + | Chr1:73520625-73520644 | MsG0180004111.01.T01:CDS | 35.0% |
| AATTCAGAGGGAGAATCCAT+TGG | + | Chr1:73520540-73520559 | MsG0180004111.01.T01:CDS | 40.0% | |
| ACAATGATGGCATCAACCAA+TGG | - | Chr1:73520559-73520578 | None:intergenic | 40.0% | |
| AGCAGAAGCTACAATTCAGA+GGG | + | Chr1:73520528-73520547 | MsG0180004111.01.T01:CDS | 40.0% | |
| AGTTCCTTCTAGCACAATGA+TGG | - | Chr1:73520572-73520591 | None:intergenic | 40.0% | |
| GTCTGATGAATGCAAAGGTA+AGG | + | Chr1:73520327-73520346 | MsG0180004111.01.T01:intron | 40.0% | |
| TAAGAGTTCATGGCCAGAAT+TGG | + | Chr1:73520486-73520505 | MsG0180004111.01.T01:CDS | 40.0% | |
| TAGCAGAAGCTACAATTCAG+AGG | + | Chr1:73520527-73520546 | MsG0180004111.01.T01:CDS | 40.0% | |
| AGTTCATGGCCAGAATTGGT+TGG | + | Chr1:73520490-73520509 | MsG0180004111.01.T01:CDS | 45.0% | |
| ATTGGTTGGAGTGGAAGGAA+AGG | + | Chr1:73520504-73520523 | MsG0180004111.01.T01:CDS | 45.0% | |
| GATGCCATCATTGTGCTAGA+AGG | + | Chr1:73520565-73520584 | MsG0180004111.01.T01:CDS | 45.0% | |
| TGTGGTCAGGTAAGAGTTCA+TGG | + | Chr1:73520476-73520495 | MsG0180004111.01.T01:intron | 45.0% | |
| AAACCCAAACCCTGTCACAG+CGG | - | Chr1:73520612-73520631 | None:intergenic | 50.0% | |
| ATGGCCAGAATTGGTTGGAG+TGG | + | Chr1:73520495-73520514 | MsG0180004111.01.T01:CDS | 50.0% | |
| CCAGAATTGGTTGGAGTGGA+AGG | + | Chr1:73520499-73520518 | MsG0180004111.01.T01:CDS | 50.0% | |
| CCTTCCACTCCAACCAATTC+TGG | - | Chr1:73520502-73520521 | None:intergenic | 50.0% | |
| CTGTCACAGCGGAAGTCAAA+GGG | - | Chr1:73520601-73520620 | None:intergenic | 50.0% | |
| TGTCACAGCGGAAGTCAAAG+GGG | - | Chr1:73520600-73520619 | None:intergenic | 50.0% | |
| ! | CTGTGACAGGGTTTGGGTTT+GGG | + | Chr1:73520612-73520631 | MsG0180004111.01.T01:CDS | 50.0% |
| ! | CTTTGACTTCCGCTGTGACA+GGG | + | Chr1:73520600-73520619 | MsG0180004111.01.T01:CDS | 50.0% |
| ACTTCCGCTGTGACAGGGTT+TGG | + | Chr1:73520605-73520624 | MsG0180004111.01.T01:CDS | 55.0% | |
| CCTGTCACAGCGGAAGTCAA+AGG | - | Chr1:73520602-73520621 | None:intergenic | 55.0% | |
| CTTCCGCTGTGACAGGGTTT+GGG | + | Chr1:73520606-73520625 | MsG0180004111.01.T01:CDS | 55.0% | |
| ! | CCTTTGACTTCCGCTGTGAC+AGG | + | Chr1:73520599-73520618 | MsG0180004111.01.T01:CDS | 55.0% |
| ! | GCTGTGACAGGGTTTGGGTT+TGG | + | Chr1:73520611-73520630 | MsG0180004111.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 73520325 | 73520877 | 73520325 | ID=MsG0180004111.01;Name=MsG0180004111.01 |
| Chr1 | mRNA | 73520325 | 73520877 | 73520325 | ID=MsG0180004111.01.T01;Parent=MsG0180004111.01;Name=MsG0180004111.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0|0.5|1|1|1|2|199|70 |
| Chr1 | exon | 73520325 | 73520343 | 73520325 | ID=MsG0180004111.01.T01:exon:31318;Parent=MsG0180004111.01.T01 |
| Chr1 | exon | 73520485 | 73520877 | 73520485 | ID=MsG0180004111.01.T01:exon:31319;Parent=MsG0180004111.01.T01 |
| Chr1 | CDS | 73520325 | 73520343 | 73520325 | ID=MsG0180004111.01.T01:cds;Parent=MsG0180004111.01.T01 |
| Chr1 | CDS | 73520485 | 73520678 | 73520485 | ID=MsG0180004111.01.T01:cds;Parent=MsG0180004111.01.T01 |
| Chr1 | three_prime_UTR | 73520679 | 73520877 | 73520679 | ID=MsG0180004111.01.T01:three_prime_utr;Parent=MsG0180004111.01.T01 |
| Gene Sequence |
| Protein sequence |
