Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006974.01.T01 | XP_013462645.1 | 95.455 | 132 | 1 | 1 | 1 | 132 | 1 | 127 | 1.01E-84 | 253 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006974.01.T01 | A0A072VET2 | 95.455 | 132 | 1 | 1 | 1 | 132 | 1 | 127 | 4.84e-85 | 253 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004110.01 | MsG0280006974.01 | 0.885746 | 6.233421e-72 | 4.330541e-68 |
| MsG0180004111.01 | MsG0280006974.01 | 0.862477 | 4.929882e-64 | 1.445858e-60 |
| MsG0180004770.01 | MsG0280006974.01 | 0.821116 | 4.791751e-53 | 3.863633e-50 |
| MsG0180004867.01 | MsG0280006974.01 | 0.842674 | 2.238313e-58 | 3.418661e-55 |
| MsG0180005813.01 | MsG0280006974.01 | 0.809760 | 1.613364e-50 | 9.554529e-48 |
| MsG0180006012.01 | MsG0280006974.01 | 0.813373 | 2.645284e-51 | 1.724490e-48 |
| MsG0180006047.01 | MsG0280006974.01 | 0.832746 | 7.926185e-56 | 8.941211e-53 |
| MsG0280006974.01 | MsG0280007073.01 | 0.803149 | 4.000379e-49 | 1.995430e-46 |
| MsG0280006974.01 | MsG0280007253.01 | 0.867556 | 1.254520e-65 | 4.398644e-62 |
| MsG0280006974.01 | MsG0280009394.01 | 0.802357 | 5.830059e-49 | 2.850110e-46 |
| MsG0280006974.01 | MsG0280011457.01 | 0.802588 | 5.224805e-49 | 2.569161e-46 |
| MsG0280006974.01 | MsG0480018333.01 | 0.819668 | 1.029316e-52 | 7.971869e-50 |
| MsG0280006974.01 | MsG0480019295.01 | 0.827687 | 1.363552e-54 | 1.325850e-51 |
| MsG0280006974.01 | MsG0480019880.01 | 0.823472 | 1.359620e-53 | 1.171792e-50 |
| MsG0280006974.01 | MsG0480022152.01 | 0.842328 | 2.765638e-58 | 4.178939e-55 |
| MsG0280006974.01 | MsG0480023318.01 | 0.821870 | 3.208066e-53 | 2.642063e-50 |
| MsG0280006974.01 | MsG0480023947.01 | 0.838457 | 2.844168e-57 | 3.811779e-54 |
| MsG0280006974.01 | MsG0580024407.01 | 0.835946 | 1.247908e-56 | 1.549350e-53 |
| MsG0280006974.01 | MsG0580024575.01 | 0.810809 | 9.581856e-51 | 5.833340e-48 |
| MsG0280006974.01 | MsG0580027733.01 | 0.809244 | 2.082446e-50 | 1.216410e-47 |
| MsG0280006974.01 | MsG0580029583.01 | 0.803714 | 3.056179e-49 | 1.546664e-46 |
| MsG0280006974.01 | MsG0580029930.01 | 0.825893 | 3.656452e-54 | 3.376455e-51 |
| MsG0280006974.01 | MsG0680031055.01 | 0.813594 | 2.365151e-51 | 1.551134e-48 |
| MsG0280006974.01 | MsG0780038921.01 | 0.805432 | 1.339017e-49 | 7.082951e-47 |
| MsG0280006974.01 | MsG0780038925.01 | 0.823439 | 1.384204e-53 | 1.191829e-50 |
| MsG0280006974.01 | MsG0780039250.01 | 0.813558 | 2.408242e-51 | 1.577823e-48 |
| MsG0280006974.01 | MsG0780039877.01 | 0.806931 | 6.471255e-50 | 3.557245e-47 |
| MsG0280006974.01 | MsG0780039985.01 | 0.800474 | 1.417044e-48 | 6.604032e-46 |
| MsG0280006974.01 | MsG0780041357.01 | 0.805970 | 1.032196e-49 | 5.536158e-47 |
| MsG0280006974.01 | MsG0880042843.01 | 0.811523 | 6.709917e-51 | 4.162893e-48 |
| MsG0280006974.01 | MsG0880043094.01 | 0.816279 | 6.002285e-52 | 4.234867e-49 |
| MsG0280006974.01 | MsG0880043285.01 | 0.860659 | 1.770171e-63 | 4.876018e-60 |
| MsG0280006974.01 | MsG0880043745.01 | 0.820687 | 6.014408e-53 | 4.791540e-50 |
| MsG0280006974.01 | MsG0880045441.01 | 0.800494 | 1.403520e-48 | 6.544550e-46 |
| MsG0280006974.01 | MsG0880045819.01 | 0.805591 | 1.239926e-49 | 6.585644e-47 |
| MsG0280006974.01 | MsG0880047375.01 | 0.817718 | 2.851628e-52 | 2.092724e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006974.01.T01 | MTR_2g018175 | 95.455 | 132 | 1 | 1 | 1 | 132 | 1 | 127 | 1.23e-88 | 253 |
| MsG0280006974.01.T01 | MTR_2g023390 | 50.602 | 83 | 33 | 3 | 57 | 131 | 74 | 156 | 3.26e-16 | 70.9 |
| MsG0280006974.01.T01 | MTR_3g052330 | 61.224 | 49 | 17 | 1 | 85 | 131 | 150 | 198 | 1.39e-12 | 62.0 |
| MsG0280006974.01.T01 | MTR_2g094410 | 40.816 | 98 | 44 | 4 | 43 | 129 | 60 | 154 | 1.31e-11 | 58.9 |
| MsG0280006974.01.T01 | MTR_7g105130 | 56.250 | 48 | 19 | 1 | 86 | 131 | 170 | 217 | 9.38e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006974.01.T01 | AT1G29640 | 40.000 | 145 | 69 | 6 | 1 | 132 | 1 | 140 | 6.86e-25 | 92.8 |
| MsG0280006974.01.T01 | AT2G34340 | 39.860 | 143 | 68 | 5 | 1 | 132 | 1 | 136 | 1.40e-23 | 89.4 |
| MsG0280006974.01.T01 | AT5G45630 | 34.532 | 139 | 59 | 4 | 1 | 132 | 1 | 114 | 8.11e-20 | 79.0 |
| MsG0280006974.01.T01 | AT4G21970 | 46.667 | 90 | 29 | 3 | 57 | 131 | 60 | 145 | 2.17e-18 | 76.6 |
| MsG0280006974.01.T01 | AT4G18980 | 42.222 | 135 | 46 | 7 | 1 | 132 | 1 | 106 | 6.08e-18 | 73.9 |
| MsG0280006974.01.T01 | AT4G04630 | 64.000 | 50 | 16 | 1 | 84 | 131 | 119 | 168 | 5.57e-15 | 67.8 |
| MsG0280006974.01.T01 | AT3G15040 | 56.863 | 51 | 20 | 1 | 83 | 131 | 193 | 243 | 9.53e-12 | 60.5 |
Find 46 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGTTAGGAATTCGATTCTT+AGG | 0.266852 | 2:+8861149 | MsG0280006974.01.T01:CDS |
| CAGCTTCAGATTGATGAATC+TGG | 0.295768 | 2:-8860838 | None:intergenic |
| CTAGCCTCCGCGCCATAATC+AGG | 0.322627 | 2:-8861060 | None:intergenic |
| GAAGGGATTTGAGCAGGGTT+AGG | 0.370718 | 2:+8861134 | MsG0280006974.01.T01:CDS |
| GGTGCCGCCGCACCTGATTA+TGG | 0.386353 | 2:+8861048 | MsG0280006974.01.T01:CDS |
| TGAAGGGAAGGGATTTGAGC+AGG | 0.444324 | 2:+8861128 | MsG0280006974.01.T01:CDS |
| ATTATGGCGCGGAGGCTAGC+TGG | 0.455420 | 2:+8861064 | MsG0280006974.01.T01:CDS |
| ATATTTCACCGGAGGGGTAA+TGG | 0.465755 | 2:+8860995 | MsG0280006974.01.T01:CDS |
| AATTCGATTCTTAGGATGAC+TGG | 0.471954 | 2:+8861157 | MsG0280006974.01.T01:CDS |
| CCATGTGTTCTGGGCATGGA+AGG | 0.480561 | 2:+8861101 | MsG0280006974.01.T01:CDS |
| TGGGCATGGAAGGACTTTGA+AGG | 0.498075 | 2:+8861111 | MsG0280006974.01.T01:CDS |
| GGTGCTGCTGAAGCTGAAGA+AGG | 0.521781 | 2:+8860871 | MsG0280006974.01.T01:CDS |
| CTATGAGGAGGACGAGGATG+AGG | 0.526881 | 2:+8861018 | MsG0280006974.01.T01:CDS |
| CCGGAGGGGTAATGGCTATG+AGG | 0.530842 | 2:+8861003 | MsG0280006974.01.T01:CDS |
| GGACGAGGATGAGGAGGAGA+TGG | 0.533776 | 2:+8861027 | MsG0280006974.01.T01:CDS |
| CCTCATAGCCATTACCCCTC+CGG | 0.534896 | 2:-8861003 | None:intergenic |
| CCCTCTGAGGGGATATTCAT+CGG | 0.539053 | 2:-8860971 | None:intergenic |
| CCTTCCATGCCCAGAACACA+TGG | 0.539401 | 2:-8861101 | None:intergenic |
| TGGAAGGACTTTGAAGGGAA+GGG | 0.555164 | 2:+8861117 | MsG0280006974.01.T01:CDS |
| CAACAACAACAAGAAGAAGA+TGG | 0.556088 | 2:+8860937 | MsG0280006974.01.T01:CDS |
| CCTCAGAGGGGATATTTCAC+CGG | 0.559919 | 2:+8860984 | MsG0280006974.01.T01:CDS |
| TGAGGAGTTTCTTGAATCCG+AGG | 0.568990 | 2:+8860802 | MsG0280006974.01.T01:CDS |
| ATCTGAAGCTGAAGACAAGA+AGG | 0.569372 | 2:+8860850 | MsG0280006974.01.T01:CDS |
| GGGCATGGAAGGACTTTGAA+GGG | 0.570682 | 2:+8861112 | MsG0280006974.01.T01:CDS |
| TGAGGGGATATTCATCGGCA+TGG | 0.573297 | 2:-8860966 | None:intergenic |
| ATGGAAGGACTTTGAAGGGA+AGG | 0.573855 | 2:+8861116 | MsG0280006974.01.T01:CDS |
| CGGTGAAATATCCCCTCTGA+GGG | 0.581904 | 2:-8860983 | None:intergenic |
| CCGATGAATATCCCCTCAGA+GGG | 0.585354 | 2:+8860971 | MsG0280006974.01.T01:CDS |
| GAAGGGAAGGGATTTGAGCA+GGG | 0.588262 | 2:+8861129 | MsG0280006974.01.T01:CDS |
| TAATGGCTATGAGGAGGACG+AGG | 0.588381 | 2:+8861012 | MsG0280006974.01.T01:CDS |
| TAACTTCACCAACATGTCTG+AGG | 0.596960 | 2:+8860784 | None:intergenic |
| TGAGGAGGACGAGGATGAGG+AGG | 0.598626 | 2:+8861021 | MsG0280006974.01.T01:CDS |
| CGCGCCATAATCAGGTGCGG+CGG | 0.605054 | 2:-8861052 | None:intergenic |
| CGCCGCACCTGATTATGGCG+CGG | 0.605485 | 2:+8861053 | MsG0280006974.01.T01:CDS |
| GCTGCTGAAGCTGAAGAAGG+AGG | 0.612278 | 2:+8860874 | MsG0280006974.01.T01:CDS |
| CTCCGCGCCATAATCAGGTG+CGG | 0.621040 | 2:-8861055 | None:intergenic |
| CCGGTGAAATATCCCCTCTG+AGG | 0.632955 | 2:-8860984 | None:intergenic |
| AGAGGGGATATTTCACCGGA+GGG | 0.643919 | 2:+8860988 | MsG0280006974.01.T01:CDS |
| CAGAGGGGATATTTCACCGG+AGG | 0.645698 | 2:+8860987 | MsG0280006974.01.T01:CDS |
| CGCACCTGATTATGGCGCGG+AGG | 0.650193 | 2:+8861056 | MsG0280006974.01.T01:CDS |
| GCCGATGAATATCCCCTCAG+AGG | 0.662649 | 2:+8860970 | MsG0280006974.01.T01:CDS |
| GAGGGGTAATGGCTATGAGG+AGG | 0.676604 | 2:+8861006 | MsG0280006974.01.T01:CDS |
| ACAACAAGAAGAAGATGGTG+AGG | 0.679378 | 2:+8860942 | MsG0280006974.01.T01:CDS |
| GAGGGGATATTTCACCGGAG+GGG | 0.727432 | 2:+8860989 | MsG0280006974.01.T01:CDS |
| CGATGAATATCCCCTCAGAG+GGG | 0.754143 | 2:+8860972 | MsG0280006974.01.T01:CDS |
| GGTGAAATATCCCCTCTGAG+GGG | 0.755545 | 2:-8860982 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATTCGATTCTTAGGATGAC+TGG | + | Chr2:8861157-8861176 | MsG0280006974.01.T01:CDS | 35.0% | |
| CAACAACAACAAGAAGAAGA+TGG | + | Chr2:8860937-8860956 | MsG0280006974.01.T01:CDS | 35.0% | |
| CTGGTTGAAAAAAAGAACCT+CGG | - | Chr2:8860822-8860841 | None:intergenic | 35.0% | |
| ! | ATGACATTTTCCATGTGTTC+TGG | + | Chr2:8861091-8861110 | MsG0280006974.01.T01:CDS | 35.0% |
| ! | TGACATTTTCCATGTGTTCT+GGG | + | Chr2:8861092-8861111 | MsG0280006974.01.T01:CDS | 35.0% |
| !!! | TAGGATGACTGGTTTTATTG+AGG | + | Chr2:8861168-8861187 | MsG0280006974.01.T01:CDS | 35.0% |
| ACAACAAGAAGAAGATGGTG+AGG | + | Chr2:8860942-8860961 | MsG0280006974.01.T01:CDS | 40.0% | |
| CAGCTTCAGATTGATGAATC+TGG | - | Chr2:8860841-8860860 | None:intergenic | 40.0% | |
| GGGTTAGGAATTCGATTCTT+AGG | + | Chr2:8861149-8861168 | MsG0280006974.01.T01:CDS | 40.0% | |
| ! | ATCTGAAGCTGAAGACAAGA+AGG | + | Chr2:8860850-8860869 | MsG0280006974.01.T01:CDS | 40.0% |
| ATATTTCACCGGAGGGGTAA+TGG | + | Chr2:8860995-8861014 | MsG0280006974.01.T01:CDS | 45.0% | |
| TGAGGAGTTTCTTGAATCCG+AGG | + | Chr2:8860802-8860821 | MsG0280006974.01.T01:CDS | 45.0% | |
| ! | ATGGAAGGACTTTGAAGGGA+AGG | + | Chr2:8861116-8861135 | MsG0280006974.01.T01:CDS | 45.0% |
| ! | TGGAAGGACTTTGAAGGGAA+GGG | + | Chr2:8861117-8861136 | MsG0280006974.01.T01:CDS | 45.0% |
| !! | TTTTCCATGTGTTCTGGGCA+TGG | + | Chr2:8861097-8861116 | MsG0280006974.01.T01:CDS | 45.0% |
| AGAGGGGATATTTCACCGGA+GGG | + | Chr2:8860988-8861007 | MsG0280006974.01.T01:CDS | 50.0% | |
| CCGATGAATATCCCCTCAGA+GGG | + | Chr2:8860971-8860990 | MsG0280006974.01.T01:CDS | 50.0% | |
| CCTCAGAGGGGATATTTCAC+CGG | + | Chr2:8860984-8861003 | MsG0280006974.01.T01:CDS | 50.0% | |
| CGATGAATATCCCCTCAGAG+GGG | + | Chr2:8860972-8860991 | MsG0280006974.01.T01:CDS | 50.0% | |
| CGGTGAAATATCCCCTCTGA+GGG | - | Chr2:8860986-8861005 | None:intergenic | 50.0% | |
| GAAGGGAAGGGATTTGAGCA+GGG | + | Chr2:8861129-8861148 | MsG0280006974.01.T01:CDS | 50.0% | |
| GAAGGGATTTGAGCAGGGTT+AGG | + | Chr2:8861134-8861153 | MsG0280006974.01.T01:CDS | 50.0% | |
| GGGCATGGAAGGACTTTGAA+GGG | + | Chr2:8861112-8861131 | MsG0280006974.01.T01:CDS | 50.0% | |
| GGTGAAATATCCCCTCTGAG+GGG | - | Chr2:8860985-8861004 | None:intergenic | 50.0% | |
| TAATGGCTATGAGGAGGACG+AGG | + | Chr2:8861012-8861031 | MsG0280006974.01.T01:CDS | 50.0% | |
| TGAAGGGAAGGGATTTGAGC+AGG | + | Chr2:8861128-8861147 | MsG0280006974.01.T01:CDS | 50.0% | |
| TGAGGGGATATTCATCGGCA+TGG | - | Chr2:8860969-8860988 | None:intergenic | 50.0% | |
| TGGGCATGGAAGGACTTTGA+AGG | + | Chr2:8861111-8861130 | MsG0280006974.01.T01:CDS | 50.0% | |
| ! | CCCTCTGAGGGGATATTCAT+CGG | - | Chr2:8860974-8860993 | None:intergenic | 50.0% |
| CAGAGGGGATATTTCACCGG+AGG | + | Chr2:8860987-8861006 | MsG0280006974.01.T01:CDS | 55.0% | |
| CCGGTGAAATATCCCCTCTG+AGG | - | Chr2:8860987-8861006 | None:intergenic | 55.0% | |
| CCTCATAGCCATTACCCCTC+CGG | - | Chr2:8861006-8861025 | None:intergenic | 55.0% | |
| CCTTCCATGCCCAGAACACA+TGG | - | Chr2:8861104-8861123 | None:intergenic | 55.0% | |
| CTATGAGGAGGACGAGGATG+AGG | + | Chr2:8861018-8861037 | MsG0280006974.01.T01:CDS | 55.0% | |
| GAGGGGATATTTCACCGGAG+GGG | + | Chr2:8860989-8861008 | MsG0280006974.01.T01:CDS | 55.0% | |
| GCCGATGAATATCCCCTCAG+AGG | + | Chr2:8860970-8860989 | MsG0280006974.01.T01:CDS | 55.0% | |
| GCTGCTGAAGCTGAAGAAGG+AGG | + | Chr2:8860874-8860893 | MsG0280006974.01.T01:CDS | 55.0% | |
| ! | CCATGTGTTCTGGGCATGGA+AGG | + | Chr2:8861101-8861120 | MsG0280006974.01.T01:CDS | 55.0% |
| ! | GAGGGGTAATGGCTATGAGG+AGG | + | Chr2:8861006-8861025 | MsG0280006974.01.T01:CDS | 55.0% |
| !! | GGTGCTGCTGAAGCTGAAGA+AGG | + | Chr2:8860871-8860890 | MsG0280006974.01.T01:CDS | 55.0% |
| ATTATGGCGCGGAGGCTAGC+TGG | + | Chr2:8861064-8861083 | MsG0280006974.01.T01:CDS | 60.0% | |
| CTAGCCTCCGCGCCATAATC+AGG | - | Chr2:8861063-8861082 | None:intergenic | 60.0% | |
| CTCCGCGCCATAATCAGGTG+CGG | - | Chr2:8861058-8861077 | None:intergenic | 60.0% | |
| GGACGAGGATGAGGAGGAGA+TGG | + | Chr2:8861027-8861046 | MsG0280006974.01.T01:CDS | 60.0% | |
| TGAGGAGGACGAGGATGAGG+AGG | + | Chr2:8861021-8861040 | MsG0280006974.01.T01:CDS | 60.0% | |
| ! | CCGGAGGGGTAATGGCTATG+AGG | + | Chr2:8861003-8861022 | MsG0280006974.01.T01:CDS | 60.0% |
| CGCACCTGATTATGGCGCGG+AGG | + | Chr2:8861056-8861075 | MsG0280006974.01.T01:CDS | 65.0% | |
| CGCCGCACCTGATTATGGCG+CGG | + | Chr2:8861053-8861072 | MsG0280006974.01.T01:CDS | 65.0% | |
| CGCGCCATAATCAGGTGCGG+CGG | - | Chr2:8861055-8861074 | None:intergenic | 65.0% | |
| ! | GGTGCCGCCGCACCTGATTA+TGG | + | Chr2:8861048-8861067 | MsG0280006974.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 8860797 | 8861195 | 8860797 | ID=MsG0280006974.01;Name=MsG0280006974.01 |
| Chr2 | mRNA | 8860797 | 8861195 | 8860797 | ID=MsG0280006974.01.T01;Parent=MsG0280006974.01;Name=MsG0280006974.01.T01;_AED=0.31;_eAED=0.31;_QI=0|-1|0|1|-1|1|1|0|132 |
| Chr2 | exon | 8860797 | 8861195 | 8860797 | ID=MsG0280006974.01.T01:exon:5805;Parent=MsG0280006974.01.T01 |
| Chr2 | CDS | 8860797 | 8861195 | 8860797 | ID=MsG0280006974.01.T01:cds;Parent=MsG0280006974.01.T01 |
| Gene Sequence |
| Protein sequence |
