Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017550.01.T01 | XP_003603540.1 | 98.485 | 66 | 1 | 0 | 1 | 66 | 1 | 66 | 4.78E-41 | 138 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017550.01.T01 | G7JCD8 | 98.485 | 66 | 1 | 0 | 1 | 66 | 1 | 66 | 2.28e-41 | 138 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048904.01 | MsG0380017550.01 | 0.813670 | 2.275928e-51 | 1.495671e-48 |
| MsG0180000337.01 | MsG0380017550.01 | 0.813559 | 2.407802e-51 | 1.577553e-48 |
| MsG0180003338.01 | MsG0380017550.01 | 0.805668 | 1.194548e-49 | 6.357323e-47 |
| MsG0180003601.01 | MsG0380017550.01 | 0.803873 | 2.832370e-49 | 1.439397e-46 |
| MsG0180004110.01 | MsG0380017550.01 | 0.814075 | 1.853040e-51 | 1.231214e-48 |
| MsG0180004562.01 | MsG0380017550.01 | 0.811072 | 8.406940e-51 | 5.153966e-48 |
| MsG0180004752.01 | MsG0380017550.01 | 0.830152 | 3.448520e-55 | 3.603568e-52 |
| MsG0180004770.01 | MsG0380017550.01 | 0.801494 | 8.767334e-49 | 4.192965e-46 |
| MsG0180004777.01 | MsG0380017550.01 | 0.818807 | 1.616304e-52 | 1.222218e-49 |
| MsG0180006012.01 | MsG0380017550.01 | 0.818757 | 1.659446e-52 | 1.253167e-49 |
| MsG0380013272.01 | MsG0380017550.01 | 0.808908 | 2.457852e-50 | 1.422721e-47 |
| MsG0380017550.01 | MsG0480018333.01 | 0.824375 | 8.350415e-54 | 7.382915e-51 |
| MsG0380017550.01 | MsG0480019295.01 | 0.875328 | 3.360500e-68 | 1.562541e-64 |
| MsG0380017550.01 | MsG0480020411.01 | 0.851758 | 7.204694e-61 | 1.473183e-57 |
| MsG0380017550.01 | MsG0580025440.01 | 0.887928 | 9.268393e-73 | 7.030930e-69 |
| MsG0380017550.01 | MsG0580027733.01 | 0.827874 | 1.229387e-54 | 1.202016e-51 |
| MsG0380017550.01 | MsG0580028999.01 | 0.818753 | 1.662379e-52 | 1.255231e-49 |
| MsG0380017550.01 | MsG0580029583.01 | 0.814864 | 1.239738e-51 | 8.414400e-49 |
| MsG0380017550.01 | MsG0780038992.01 | 0.836685 | 8.094699e-57 | 1.027710e-53 |
| MsG0380017550.01 | MsG0780039877.01 | 0.852022 | 6.063118e-61 | 1.250133e-57 |
| MsG0380017550.01 | MsG0780040144.01 | 0.807572 | 4.734236e-50 | 2.645991e-47 |
| MsG0380017550.01 | MsG0780040353.01 | 0.801872 | 7.335344e-49 | 3.541928e-46 |
| MsG0380017550.01 | MsG0880043744.01 | 0.809691 | 1.669845e-50 | 9.870445e-48 |
| MsG0380017550.01 | MsG0880043828.01 | 0.802863 | 4.584607e-49 | 2.270240e-46 |
| MsG0380017550.01 | MsG0880044769.01 | 0.832728 | 8.010144e-56 | 9.031186e-53 |
| MsG0380017550.01 | MsG0880045272.01 | 0.802931 | 4.439568e-49 | 2.202151e-46 |
| MsG0380017550.01 | MsG0880045277.01 | 0.806956 | 6.392999e-50 | 3.516471e-47 |
| MsG0380017550.01 | MsG0880046713.01 | 0.821962 | 3.054883e-53 | 2.522294e-50 |
| MsG0380017550.01 | MsG0880046996.01 | 0.819928 | 8.975399e-53 | 7.002247e-50 |
| MsG0380017550.01 | MsG0880047486.01 | 0.813638 | 2.313158e-51 | 1.518787e-48 |
| MsG0280006883.01 | MsG0380017550.01 | 0.800573 | 1.352744e-48 | 6.320367e-46 |
| MsG0280009394.01 | MsG0380017550.01 | 0.846824 | 1.704270e-59 | 2.970123e-56 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017550.01.T01 | MTR_3g108890 | 98.485 | 66 | 1 | 0 | 1 | 66 | 1 | 66 | 5.79e-45 | 138 |
| MsG0380017550.01.T01 | MTR_2g038220 | 46.970 | 66 | 35 | 0 | 1 | 66 | 37 | 102 | 1.19e-21 | 80.5 |
| MsG0380017550.01.T01 | MTR_4g127190 | 47.692 | 65 | 34 | 0 | 1 | 65 | 1 | 65 | 5.24e-20 | 75.5 |
| MsG0380017550.01.T01 | MTR_4g127200 | 47.692 | 65 | 34 | 0 | 1 | 65 | 1 | 65 | 1.14e-19 | 74.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017550.01.T01 | AT5G10695 | 63.077 | 65 | 24 | 0 | 1 | 65 | 1 | 65 | 4.66e-29 | 98.2 |
| MsG0380017550.01.T01 | AT5G57123 | 58.462 | 65 | 27 | 0 | 1 | 65 | 1 | 65 | 3.04e-24 | 86.3 |
| MsG0380017550.01.T01 | AT4G29905 | 52.308 | 65 | 31 | 0 | 1 | 65 | 1 | 65 | 8.47e-21 | 77.4 |
Find 19 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCGTTTGGTCTTGTGGTACA+AGG | 0.398618 | 3:-97782045 | None:intergenic |
| CTGGGTCGTGTCCTTATTGT+GGG | 0.403271 | 3:+97781970 | MsG0380017550.01.T01:CDS |
| GTGTCAAGGCAGCAAGCACC+TGG | 0.433792 | 3:+97781951 | MsG0380017550.01.T01:CDS |
| CCTGGGTCGTGTCCTTATTG+TGG | 0.469178 | 3:+97781969 | MsG0380017550.01.T01:CDS |
| AGTGCCTGCGTTTGGTCTTG+TGG | 0.531939 | 3:-97782052 | None:intergenic |
| ATGTGTTTAGTGTTTGTGTG+TGG | 0.565529 | 3:+97781912 | MsG0380017550.01.T01:CDS |
| GGTGCAATAGTGCCTGCGTT+TGG | 0.567231 | 3:-97782060 | None:intergenic |
| CGTTTGGTCTTGTGGTACAA+GGG | 0.591494 | 3:-97782044 | None:intergenic |
| TTTAGTGTTTGTGTGTGGAG+AGG | 0.592181 | 3:+97781917 | MsG0380017550.01.T01:CDS |
| ATTCAGGCTGTGGACGTAGA+CGG | 0.595597 | 3:+97781999 | MsG0380017550.01.T01:CDS |
| TGGGTCGTGTCCTTATTGTG+GGG | 0.611223 | 3:+97781971 | MsG0380017550.01.T01:CDS |
| CTGTGGACGTAGACGGCCGG+TGG | 0.618821 | 3:+97782006 | MsG0380017550.01.T01:CDS |
| GGGTCGTGTCCTTATTGTGG+GGG | 0.621404 | 3:+97781972 | MsG0380017550.01.T01:CDS |
| AGGCTGTGGACGTAGACGGC+CGG | 0.632905 | 3:+97782003 | MsG0380017550.01.T01:CDS |
| AGGATGAAAGAGTTGTGTCA+AGG | 0.635892 | 3:+97781937 | MsG0380017550.01.T01:CDS |
| CCACAATAAGGACACGACCC+AGG | 0.664635 | 3:-97781969 | None:intergenic |
| GTACGAAACAGAAATTCCAC+CGG | 0.682806 | 3:-97782022 | None:intergenic |
| TGTCAAGGCAGCAAGCACCT+GGG | 0.683923 | 3:+97781952 | MsG0380017550.01.T01:CDS |
| TGTACCACAAGACCAAACGC+AGG | 0.741937 | 3:+97782048 | MsG0380017550.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTATTTGACAACTAGTTTTC+TGG | - | Chr3:97782093-97782112 | None:intergenic | 30.0% |
| ATGTGTTTAGTGTTTGTGTG+TGG | + | Chr3:97781912-97781931 | MsG0380017550.01.T01:CDS | 35.0% | |
| !!! | AACTAGTTTTCTGGAACACA+TGG | - | Chr3:97782084-97782103 | None:intergenic | 35.0% |
| AGGATGAAAGAGTTGTGTCA+AGG | + | Chr3:97781937-97781956 | MsG0380017550.01.T01:CDS | 40.0% | |
| GTACGAAACAGAAATTCCAC+CGG | - | Chr3:97782025-97782044 | None:intergenic | 40.0% | |
| TGAATCATACCCCCACAATA+AGG | - | Chr3:97781984-97782003 | None:intergenic | 40.0% | |
| TTTAGTGTTTGTGTGTGGAG+AGG | + | Chr3:97781917-97781936 | MsG0380017550.01.T01:CDS | 40.0% | |
| ! | TTATTGTGGGGGTATGATTC+AGG | + | Chr3:97781983-97782002 | MsG0380017550.01.T01:CDS | 40.0% |
| !! | CGTTTGGTCTTGTGGTACAA+GGG | - | Chr3:97782047-97782066 | None:intergenic | 45.0% |
| ATTCAGGCTGTGGACGTAGA+CGG | + | Chr3:97781999-97782018 | MsG0380017550.01.T01:CDS | 50.0% | |
| CTGGGTCGTGTCCTTATTGT+GGG | + | Chr3:97781970-97781989 | MsG0380017550.01.T01:CDS | 50.0% | |
| TGGGTCGTGTCCTTATTGTG+GGG | + | Chr3:97781971-97781990 | MsG0380017550.01.T01:CDS | 50.0% | |
| TGTACCACAAGACCAAACGC+AGG | + | Chr3:97782048-97782067 | MsG0380017550.01.T01:CDS | 50.0% | |
| !! | GCGTTTGGTCTTGTGGTACA+AGG | - | Chr3:97782048-97782067 | None:intergenic | 50.0% |
| CCACAATAAGGACACGACCC+AGG | - | Chr3:97781972-97781991 | None:intergenic | 55.0% | |
| CCTGGGTCGTGTCCTTATTG+TGG | + | Chr3:97781969-97781988 | MsG0380017550.01.T01:CDS | 55.0% | |
| GGGTCGTGTCCTTATTGTGG+GGG | + | Chr3:97781972-97781991 | MsG0380017550.01.T01:CDS | 55.0% | |
| GGTGCAATAGTGCCTGCGTT+TGG | - | Chr3:97782063-97782082 | None:intergenic | 55.0% | |
| TGTCAAGGCAGCAAGCACCT+GGG | + | Chr3:97781952-97781971 | MsG0380017550.01.T01:CDS | 55.0% | |
| ! | TGGGGGTATGATTCAGGCTG+TGG | + | Chr3:97781989-97782008 | MsG0380017550.01.T01:CDS | 55.0% |
| !! | AGTGCCTGCGTTTGGTCTTG+TGG | - | Chr3:97782055-97782074 | None:intergenic | 55.0% |
| GTGTCAAGGCAGCAAGCACC+TGG | + | Chr3:97781951-97781970 | MsG0380017550.01.T01:CDS | 60.0% | |
| AGGCTGTGGACGTAGACGGC+CGG | + | Chr3:97782003-97782022 | MsG0380017550.01.T01:CDS | 65.0% | |
| CTGTGGACGTAGACGGCCGG+TGG | + | Chr3:97782006-97782025 | MsG0380017550.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 97781912 | 97782112 | 97781912 | ID=MsG0380017550.01;Name=MsG0380017550.01 |
| Chr3 | mRNA | 97781912 | 97782112 | 97781912 | ID=MsG0380017550.01.T01;Parent=MsG0380017550.01;Name=MsG0380017550.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|66 |
| Chr3 | exon | 97781912 | 97782112 | 97781912 | ID=MsG0380017550.01.T01:exon:17677;Parent=MsG0380017550.01.T01 |
| Chr3 | CDS | 97781912 | 97782112 | 97781912 | ID=MsG0380017550.01.T01:cds;Parent=MsG0380017550.01.T01 |
| Gene Sequence |
| Protein sequence |
