Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003128.01.T01 | QJD38934.1 | 95.283 | 106 | 5 | 0 | 21 | 126 | 1 | 106 | 5.69E-69 | 215 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003128.01.T01 | A0A6M3SL49 | 95.283 | 106 | 5 | 0 | 21 | 126 | 1 | 106 | 2.72e-69 | 215 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000152.01 | MsG0180003128.01 | 0.809111 | 2.224060e-50 | 1.294488e-47 |
| MsG0180000614.01 | MsG0180003128.01 | 0.804104 | 2.534899e-49 | 1.295854e-46 |
| MsG0180001795.01 | MsG0180003128.01 | 0.880481 | 5.296072e-70 | 2.989807e-66 |
| MsG0180003128.01 | MsG0180003129.01 | 0.909377 | 6.094927e-82 | 1.182067e-77 |
| MsG0180003128.01 | MsG0180004844.01 | -0.828275 | 9.844135e-55 | 9.738071e-52 |
| MsG0180003128.01 | MsG0280006411.01 | 0.809836 | 1.554046e-50 | 9.221218e-48 |
| MsG0180003128.01 | MsG0280006961.01 | -0.804650 | 1.951295e-49 | 1.011614e-46 |
| MsG0180003128.01 | MsG0280007915.01 | 0.806986 | 6.301431e-50 | 3.468770e-47 |
| MsG0180003128.01 | MsG0280010204.01 | 0.804229 | 2.387387e-49 | 1.224306e-46 |
| MsG0180003128.01 | MsG0280011120.01 | -0.808866 | 2.509564e-50 | 1.451056e-47 |
| MsG0180003128.01 | MsG0380012310.01 | 0.820178 | 7.869243e-53 | 6.182219e-50 |
| MsG0180003128.01 | MsG0380012661.01 | -0.846881 | 1.643945e-59 | 2.869973e-56 |
| MsG0180003128.01 | MsG0380013071.01 | 0.822718 | 2.039001e-53 | 1.720346e-50 |
| MsG0180003128.01 | MsG0380015400.01 | 0.810505 | 1.114964e-50 | 6.732790e-48 |
| MsG0180003128.01 | MsG0380017254.01 | 0.828746 | 7.572510e-55 | 7.593880e-52 |
| MsG0180003128.01 | MsG0480018187.01 | 0.800389 | 1.474514e-48 | 6.857317e-46 |
| MsG0180003128.01 | MsG0480018906.01 | 0.821177 | 4.636930e-53 | 3.745365e-50 |
| MsG0180003128.01 | MsG0480021249.01 | 0.866603 | 2.526925e-65 | 8.567047e-62 |
| MsG0180003128.01 | MsG0480021251.01 | 0.834544 | 2.819169e-56 | 3.356390e-53 |
| MsG0180003128.01 | MsG0580024474.01 | 0.827311 | 1.677605e-54 | 1.613686e-51 |
| MsG0180003128.01 | MsG0580025985.01 | -0.817788 | 2.749409e-52 | 2.021634e-49 |
| MsG0180003128.01 | MsG0580029166.01 | 0.841758 | 3.912293e-58 | 5.806030e-55 |
| MsG0180003128.01 | MsG0580029256.01 | 0.853139 | 2.911946e-61 | 6.228219e-58 |
| MsG0180003128.01 | MsG0580029642.01 | 0.820495 | 6.656286e-53 | 5.275055e-50 |
| MsG0180003128.01 | MsG0680032430.01 | 0.834228 | 3.383679e-56 | 3.990040e-53 |
| MsG0180003128.01 | MsG0780039841.01 | 0.833778 | 4.386454e-56 | 5.103336e-53 |
| MsG0180003128.01 | MsG0780040038.01 | 0.817083 | 3.964331e-52 | 2.859011e-49 |
| MsG0180003128.01 | MsG0880046472.01 | 0.827809 | 1.274377e-54 | 1.243539e-51 |
| MsG0180003128.01 | MsG0880047448.01 | 0.818978 | 1.478166e-52 | 1.123235e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0180003128.01 | MsG0580028235.01 | PPI |
| MsG0180003128.01 | MsG0580027943.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003128.01.T01 | MTR_1g054765 | 89.815 | 108 | 9 | 1 | 21 | 126 | 1 | 108 | 1.11e-68 | 204 |
| MsG0180003128.01.T01 | MTR_1g054750 | 72.222 | 126 | 30 | 4 | 1 | 126 | 1 | 121 | 2.76e-56 | 173 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003128.01.T01 | AT3G11930 | 46.491 | 114 | 54 | 4 | 15 | 126 | 25 | 133 | 9.01e-26 | 96.3 |
| MsG0180003128.01.T01 | AT3G11930 | 46.018 | 113 | 55 | 3 | 15 | 126 | 25 | 132 | 9.49e-26 | 96.3 |
| MsG0180003128.01.T01 | AT3G11930 | 45.690 | 116 | 53 | 4 | 15 | 126 | 25 | 134 | 1.37e-25 | 95.9 |
| MsG0180003128.01.T01 | AT3G11930 | 46.491 | 114 | 54 | 4 | 15 | 126 | 25 | 133 | 2.19e-25 | 95.9 |
| MsG0180003128.01.T01 | AT3G58450 | 39.216 | 102 | 57 | 2 | 25 | 126 | 32 | 128 | 3.26e-17 | 74.3 |
| MsG0180003128.01.T01 | AT3G58450 | 36.275 | 102 | 53 | 2 | 25 | 126 | 32 | 121 | 1.07e-14 | 67.4 |
Find 41 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTTATGGCTACTGATACTA+TGG | 0.183037 | 1:-57038560 | MsG0180003128.01.T01:CDS |
| TTCAAAGAATCTGCAACTTC+AGG | 0.297788 | 1:+57038079 | None:intergenic |
| TATGCGTAAGTGTGGAAATT+TGG | 0.310359 | 1:+57038484 | None:intergenic |
| CCAGCTGGTCCCATAGGTAT+AGG | 0.340250 | 1:+57038460 | None:intergenic |
| AAAAGGGTGATGGAGGAAAT+AGG | 0.378001 | 1:-57038712 | None:intergenic |
| TGATAACCTCTTCAACGTTA+TGG | 0.381044 | 1:-57038575 | MsG0180003128.01.T01:CDS |
| CAGCTGGTCCCATAGGTATA+GGG | 0.428625 | 1:+57038461 | None:intergenic |
| TCTGCAACTTCAGGTGTTGA+TGG | 0.429179 | 1:+57038088 | None:intergenic |
| ATCTGCAGCTATTGCCTCAC+GGG | 0.482712 | 1:-57038038 | MsG0180003128.01.T01:CDS |
| TTACGCATACCCTATACCTA+TGG | 0.486548 | 1:-57038470 | MsG0180003128.01.T01:CDS |
| AAAGAGAAGGGTGAAGATGA+AGG | 0.493136 | 1:-57038653 | MsG0180003128.01.T01:CDS |
| GAAGCTTGTATGCTCTTAAG+TGG | 0.498753 | 1:-57038603 | MsG0180003128.01.T01:CDS |
| GCAAATGTGCAAGGATAAGC+AGG | 0.503833 | 1:-57038011 | MsG0180003128.01.T01:CDS |
| AGGTGCAGAAGAAAAGAGAA+GGG | 0.511461 | 1:-57038665 | MsG0180003128.01.T01:CDS |
| TGGAAGTGTTGATCAAGTGA+GGG | 0.512230 | 1:-57038689 | MsG0180003128.01.T01:CDS |
| CCAATACCAGCTGGTCCCAT+AGG | 0.522576 | 1:+57038454 | None:intergenic |
| TAACGTTGAAGAGGTTATCA+AGG | 0.523668 | 1:+57038578 | None:intergenic |
| ATCTGAAAATGAGGAGGGGA+TGG | 0.523730 | 1:-57038527 | MsG0180003128.01.T01:CDS |
| GAGGTGCAGAAGAAAAGAGA+AGG | 0.554300 | 1:-57038666 | MsG0180003128.01.T01:CDS |
| TGGAACAACATCTGAAAATG+AGG | 0.557443 | 1:-57038536 | MsG0180003128.01.T01:CDS |
| ATGGCTACTGATACTATGGA+TGG | 0.557940 | 1:-57038556 | MsG0180003128.01.T01:CDS |
| GTGGAAGTGTTGATCAAGTG+AGG | 0.562018 | 1:-57038690 | MsG0180003128.01.T01:CDS |
| CAGTAGCCATAACGTTGAAG+AGG | 0.571035 | 1:+57038569 | None:intergenic |
| CCTATACCTATGGGACCAGC+TGG | 0.573743 | 1:-57038460 | MsG0180003128.01.T01:intron |
| GTATAGGGTATGCGTAAGTG+TGG | 0.575552 | 1:+57038476 | None:intergenic |
| GGTGAAGATGAAGGTGATGG+TGG | 0.579359 | 1:-57038644 | MsG0180003128.01.T01:CDS |
| TACGCATACCCTATACCTAT+GGG | 0.589603 | 1:-57038469 | MsG0180003128.01.T01:CDS |
| AATCTGCAGCTATTGCCTCA+CGG | 0.590946 | 1:-57038039 | MsG0180003128.01.T01:CDS |
| ACGGGCTTTGCAAATGTGCA+AGG | 0.605600 | 1:-57038020 | MsG0180003128.01.T01:CDS |
| GGAAGTGTTGATCAAGTGAG+GGG | 0.617330 | 1:-57038688 | MsG0180003128.01.T01:CDS |
| AAGGGTGAAGATGAAGGTGA+TGG | 0.619103 | 1:-57038647 | MsG0180003128.01.T01:CDS |
| ACAACATCTGAAAATGAGGA+GGG | 0.619877 | 1:-57038532 | MsG0180003128.01.T01:CDS |
| AACAACATCTGAAAATGAGG+AGG | 0.624375 | 1:-57038533 | MsG0180003128.01.T01:CDS |
| AACGTTGAAGAGGTTATCAA+GGG | 0.625008 | 1:+57038579 | None:intergenic |
| AAGCTTGTATGCTCTTAAGT+GGG | 0.653871 | 1:-57038602 | MsG0180003128.01.T01:CDS |
| GTGGCAATAGATGAGAGTGA+TGG | 0.658459 | 1:-57038625 | MsG0180003128.01.T01:CDS |
| CAACATCTGAAAATGAGGAG+GGG | 0.662503 | 1:-57038531 | MsG0180003128.01.T01:CDS |
| GCACATTTGCAAAGCCCGTG+AGG | 0.665936 | 1:+57038024 | None:intergenic |
| TGTGCAAGGATAAGCAGGTG+CGG | 0.687131 | 1:-57038006 | MsG0180003128.01.T01:CDS |
| AGGGTGATGGAGGAAATAGG+TGG | 0.693358 | 1:-57038709 | None:intergenic |
| AGTGTTGATCAAGTGAGGGG+AGG | 0.733239 | 1:-57038685 | MsG0180003128.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTAATATTGTCTATTTTTCT+TGG | - | Chr1:57038385-57038404 | MsG0180003128.01.T01:intron | 15.0% |
| !! | AAAAATGATGTAACTTTGTA+AGG | - | Chr1:57038354-57038373 | MsG0180003128.01.T01:intron | 20.0% |
| !! | GAATTACACATGTAAAAAAA+AGG | + | Chr1:57038321-57038340 | None:intergenic | 20.0% |
| AACTTACACAAGTGTATTCT+CGG | + | Chr1:57038496-57038515 | None:intergenic | 30.0% | |
| TGATGTAACTTTGTAAGGTT+TGG | - | Chr1:57038359-57038378 | MsG0180003128.01.T01:intron | 30.0% | |
| ! | CAAAGTATCACACAAGAAAA+AGG | + | Chr1:57038578-57038597 | None:intergenic | 30.0% |
| AACAACATCTGAAAATGAGG+AGG | - | Chr1:57038173-57038192 | MsG0180003128.01.T01:intron | 35.0% | |
| AACGTTGAAGAGGTTATCAA+GGG | + | Chr1:57038130-57038149 | None:intergenic | 35.0% | |
| AAGCTTGTATGCTCTTAAGT+GGG | - | Chr1:57038104-57038123 | MsG0180003128.01.T01:intron | 35.0% | |
| ACAACATCTGAAAATGAGGA+GGG | - | Chr1:57038174-57038193 | MsG0180003128.01.T01:intron | 35.0% | |
| AGGAAGTAAGCAAAGAAAGT+TGG | + | Chr1:57038301-57038320 | None:intergenic | 35.0% | |
| ATATACTAACCAATACCAGC+TGG | + | Chr1:57038264-57038283 | None:intergenic | 35.0% | |
| ATGGATAAACAGCTGTTGAA+AGG | + | Chr1:57038602-57038621 | None:intergenic | 35.0% | |
| GCAGAAATGAGAAAATCAGA+TGG | + | Chr1:57038442-57038461 | None:intergenic | 35.0% | |
| TAACGTTGAAGAGGTTATCA+AGG | + | Chr1:57038131-57038150 | None:intergenic | 35.0% | |
| TATGCGTAAGTGTGGAAATT+TGG | + | Chr1:57038225-57038244 | None:intergenic | 35.0% | |
| TGATAACCTCTTCAACGTTA+TGG | - | Chr1:57038131-57038150 | MsG0180003128.01.T01:intron | 35.0% | |
| TGGAACAACATCTGAAAATG+AGG | - | Chr1:57038170-57038189 | MsG0180003128.01.T01:intron | 35.0% | |
| TGTGTAAGTTAATTCACGCT+AGG | - | Chr1:57038506-57038525 | MsG0180003128.01.T01:CDS | 35.0% | |
| TTCAAAGAATCTGCAACTTC+AGG | + | Chr1:57038630-57038649 | None:intergenic | 35.0% | |
| AAAGAGAAGGGTGAAGATGA+AGG | - | Chr1:57038053-57038072 | MsG0180003128.01.T01:CDS | 40.0% | |
| AGGTGCAGAAGAAAAGAGAA+GGG | - | Chr1:57038041-57038060 | MsG0180003128.01.T01:CDS | 40.0% | |
| ATGGCTACTGATACTATGGA+TGG | - | Chr1:57038150-57038169 | MsG0180003128.01.T01:intron | 40.0% | |
| CAACATCTGAAAATGAGGAG+GGG | - | Chr1:57038175-57038194 | MsG0180003128.01.T01:intron | 40.0% | |
| CAGATGGAAGAAGAGCAATT+TGG | + | Chr1:57038426-57038445 | None:intergenic | 40.0% | |
| CGTTATGGCTACTGATACTA+TGG | - | Chr1:57038146-57038165 | MsG0180003128.01.T01:intron | 40.0% | |
| GAAGCTTGTATGCTCTTAAG+TGG | - | Chr1:57038103-57038122 | MsG0180003128.01.T01:intron | 40.0% | |
| GGGAACGAGCAAAAATAGAT+GGG | - | Chr1:57038539-57038558 | MsG0180003128.01.T01:CDS | 40.0% | |
| TACGCATACCCTATACCTAT+GGG | - | Chr1:57038237-57038256 | MsG0180003128.01.T01:intron | 40.0% | |
| TTACGCATACCCTATACCTA+TGG | - | Chr1:57038236-57038255 | MsG0180003128.01.T01:intron | 40.0% | |
| TTCTCGGTATGAGTTAACTC+AGG | + | Chr1:57038480-57038499 | None:intergenic | 40.0% | |
| ! | AGCTGCAGATTTTTCTTGCT+GGG | + | Chr1:57038658-57038677 | None:intergenic | 40.0% |
| ! | TAGCTGCAGATTTTTCTTGC+TGG | + | Chr1:57038659-57038678 | None:intergenic | 40.0% |
| !! | TGGAAGTGTTGATCAAGTGA+GGG | - | Chr1:57038017-57038036 | MsG0180003128.01.T01:CDS | 40.0% |
| !! | TTCACGCTAGGCTATTTTGA+CGG | - | Chr1:57038518-57038537 | MsG0180003128.01.T01:CDS | 40.0% |
| AAGGGTGAAGATGAAGGTGA+TGG | - | Chr1:57038059-57038078 | MsG0180003128.01.T01:CDS | 45.0% | |
| AATCTGCAGCTATTGCCTCA+CGG | - | Chr1:57038667-57038686 | MsG0180003128.01.T01:CDS | 45.0% | |
| ATCTGAAAATGAGGAGGGGA+TGG | - | Chr1:57038179-57038198 | MsG0180003128.01.T01:intron | 45.0% | |
| CAGTAGCCATAACGTTGAAG+AGG | + | Chr1:57038140-57038159 | None:intergenic | 45.0% | |
| CGGGAACGAGCAAAAATAGA+TGG | - | Chr1:57038538-57038557 | MsG0180003128.01.T01:CDS | 45.0% | |
| GAGGTGCAGAAGAAAAGAGA+AGG | - | Chr1:57038040-57038059 | MsG0180003128.01.T01:CDS | 45.0% | |
| GCAAATGTGCAAGGATAAGC+AGG | - | Chr1:57038695-57038714 | MsG0180003128.01.T01:CDS | 45.0% | |
| GTATAGGGTATGCGTAAGTG+TGG | + | Chr1:57038233-57038252 | None:intergenic | 45.0% | |
| GTGGCAATAGATGAGAGTGA+TGG | - | Chr1:57038081-57038100 | MsG0180003128.01.T01:CDS | 45.0% | |
| !! | GGAAGTGTTGATCAAGTGAG+GGG | - | Chr1:57038018-57038037 | MsG0180003128.01.T01:CDS | 45.0% |
| !! | GTGGAAGTGTTGATCAAGTG+AGG | - | Chr1:57038016-57038035 | MsG0180003128.01.T01:CDS | 45.0% |
| !! | TCTGCAACTTCAGGTGTTGA+TGG | + | Chr1:57038621-57038640 | None:intergenic | 45.0% |
| !!! | TCACGCTAGGCTATTTTGAC+GGG | - | Chr1:57038519-57038538 | MsG0180003128.01.T01:CDS | 45.0% |
| ACGGGCTTTGCAAATGTGCA+AGG | - | Chr1:57038686-57038705 | MsG0180003128.01.T01:CDS | 50.0% | |
| ATCTGCAGCTATTGCCTCAC+GGG | - | Chr1:57038668-57038687 | MsG0180003128.01.T01:CDS | 50.0% | |
| CAGCTGGTCCCATAGGTATA+GGG | + | Chr1:57038248-57038267 | None:intergenic | 50.0% | |
| TGTGCAAGGATAAGCAGGTG+CGG | - | Chr1:57038700-57038719 | MsG0180003128.01.T01:CDS | 50.0% | |
| ! | GGTGAAGATGAAGGTGATGG+TGG | - | Chr1:57038062-57038081 | MsG0180003128.01.T01:CDS | 50.0% |
| !! | AGTGTTGATCAAGTGAGGGG+AGG | - | Chr1:57038021-57038040 | MsG0180003128.01.T01:CDS | 50.0% |
| CCAATACCAGCTGGTCCCAT+AGG | + | Chr1:57038255-57038274 | None:intergenic | 55.0% | |
| CCAGCTGGTCCCATAGGTAT+AGG | + | Chr1:57038249-57038268 | None:intergenic | 55.0% | |
| CCTATACCTATGGGACCAGC+TGG | - | Chr1:57038246-57038265 | MsG0180003128.01.T01:intron | 55.0% | |
| CCTATGGGACCAGCTGGTAT+TGG | - | Chr1:57038252-57038271 | MsG0180003128.01.T01:intron | 55.0% | |
| GCACATTTGCAAAGCCCGTG+AGG | + | Chr1:57038685-57038704 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 57038003 | 57038725 | 57038003 | ID=MsG0180003128.01;Name=MsG0180003128.01 |
| Chr1 | mRNA | 57038003 | 57038725 | 57038003 | ID=MsG0180003128.01.T01;Parent=MsG0180003128.01;Name=MsG0180003128.01.T01;_AED=0.50;_eAED=0.51;_QI=0|0|0|1|1|1|2|0|126 |
| Chr1 | exon | 57038461 | 57038725 | 57038461 | ID=MsG0180003128.01.T01:exon:16080;Parent=MsG0180003128.01.T01 |
| Chr1 | exon | 57038003 | 57038118 | 57038003 | ID=MsG0180003128.01.T01:exon:16079;Parent=MsG0180003128.01.T01 |
| Chr1 | CDS | 57038461 | 57038725 | 57038461 | ID=MsG0180003128.01.T01:cds;Parent=MsG0180003128.01.T01 |
| Chr1 | CDS | 57038003 | 57038118 | 57038003 | ID=MsG0180003128.01.T01:cds;Parent=MsG0180003128.01.T01 |
| Gene Sequence |
| Protein sequence |
