AlfalfaGEDB | MsG0180004880.01

M. sativa cultivar ZhongmuNo.1 / MsG0180004880.01

KEGG: K00799
GO: GO:0005515
PFAM: PF00043 PF02798

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004880.01.T01	BAB70616.1	100	199	0	0	1	199	16	214	6.21E-145	412

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004880.01.T01	P46423	64.948	194	68	0	1	194	15	208	3.35E-92	271

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004880.01.T01	Q948X4	100.000	199	0	0	1	199	16	214	2.96e-145	412

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180001029.01	MsG0180004880.01	0.805710	1.170446e-49	6.236125e-47
MsG0180003320.01	MsG0180004880.01	0.802635	5.109706e-49	2.515556e-46
MsG0180003404.01	MsG0180004880.01	0.830027	3.701213e-55	3.853307e-52
MsG0180004880.01	MsG0380016490.01	0.818284	2.123638e-52	1.582849e-49
MsG0180004880.01	MsG0480020595.01	0.844428	7.607133e-59	1.227712e-55
MsG0180004880.01	MsG0680031852.01	0.823556	1.299815e-53	1.122887e-50
MsG0180004880.01	MsG0680032932.01	0.815030	1.138799e-51	7.764533e-49
MsG0180004880.01	MsG0680034238.01	0.806064	9.859789e-50	5.301009e-47
MsG0180004880.01	MsG0780036835.01	0.800226	1.591504e-48	7.370740e-46
MsG0180004880.01	MsG0780038318.01	0.808062	3.723881e-50	2.108245e-47
MsG0180004880.01	MsG0880045801.01	0.801159	1.027005e-48	4.870345e-46
MsG0180004880.01	MsG0880046946.01	0.808122	3.617522e-50	2.051260e-47

PPI

Gene1	Gene2	Type
MsG0180004881.01	MsG0180004880.01	PPI
MsG0180004880.01	MsG0180004879.01	PPI
MsG0180004879.01	MsG0180004880.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004880.01.T01	MTR_1g088845	94.472	199	11	0	1	199	16	214	3.13e-140	390
MsG0180004880.01.T01	MTR_1g088840	89.447	199	21	0	1	199	16	214	9.42e-134	374
MsG0180004880.01.T01	MTR_1g088825	89.447	199	21	0	1	199	16	214	9.42e-134	374
MsG0180004880.01.T01	MTR_1g088840	89.157	166	18	0	34	199	2	167	1.60e-108	308
MsG0180004880.01.T01	MTR_1g088840	88.136	118	14	0	82	199	1	118	1.27e-72	215
MsG0180004880.01.T01	MTR_3g450930	43.878	196	109	1	2	196	15	210	2.43e-55	175
MsG0180004880.01.T01	MTR_3g064700	45.078	193	105	1	2	193	16	208	9.84e-55	173
MsG0180004880.01.T01	MTR_3g450790	42.347	196	112	1	2	196	15	210	1.85e-53	170
MsG0180004880.01.T01	MTR_1g088850	40.000	195	116	1	2	195	16	210	6.02e-52	166
MsG0180004880.01.T01	MTR_5g090920	42.347	196	109	3	2	194	15	209	1.23e-49	160
MsG0180004880.01.T01	MTR_1g026140	40.394	203	115	3	2	198	18	220	1.89e-49	160
MsG0180004880.01.T01	MTR_5g090910	39.594	197	117	2	2	196	15	211	1.32e-47	155
MsG0180004880.01.T01	MTR_1g492670	88.235	68	8	0	132	199	2	69	1.75e-39	130
MsG0180004880.01.T01	MTR_5g090910	37.415	147	90	2	52	196	8	154	1.50e-28	105
MsG0180004880.01.T01	MTR_8g098420	29.839	124	87	0	12	135	26	149	6.22e-11	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004880.01.T01	AT1G02920	59.585	193	78	0	2	194	17	209	4.26e-79	235
MsG0180004880.01.T01	AT1G02930	58.549	193	79	1	2	194	17	208	9.05e-79	234
MsG0180004880.01.T01	AT1G02930	58.549	193	79	1	2	194	17	208	9.05e-79	234
MsG0180004880.01.T01	AT2G02930	58.673	196	78	1	2	194	17	212	1.20e-78	234
MsG0180004880.01.T01	AT4G02520	57.436	195	80	1	2	193	17	211	2.19e-78	233
MsG0180004880.01.T01	AT2G47730	54.872	195	87	1	1	194	64	258	2.04e-77	233
MsG0180004880.01.T01	AT2G47730	54.872	195	87	1	1	194	64	258	2.04e-77	233
MsG0180004880.01.T01	AT1G02950	51.579	190	91	1	2	190	40	229	9.27e-71	215
MsG0180004880.01.T01	AT1G02950	51.579	190	91	1	2	190	50	239	1.43e-70	215
MsG0180004880.01.T01	AT1G02950	51.579	190	91	1	2	190	38	227	1.44e-70	214
MsG0180004880.01.T01	AT1G02950	51.579	190	91	1	2	190	38	227	1.44e-70	214
MsG0180004880.01.T01	AT1G02940	46.392	194	103	1	2	194	52	245	6.75e-63	196
MsG0180004880.01.T01	AT1G02940	46.392	194	103	1	2	194	52	245	6.75e-63	196
MsG0180004880.01.T01	AT1G02940	46.392	194	103	1	2	194	65	258	1.12e-62	196
MsG0180004880.01.T01	AT1G02940	46.392	194	103	1	2	194	77	270	1.33e-62	196
MsG0180004880.01.T01	AT3G62760	47.668	193	100	1	2	193	16	208	6.26e-59	184
MsG0180004880.01.T01	AT1G49860	47.692	195	98	2	6	196	22	216	2.96e-57	181
MsG0180004880.01.T01	AT3G03190	44.388	196	108	1	2	196	16	211	2.72e-52	167
MsG0180004880.01.T01	AT5G17220	39.796	196	117	1	2	196	16	211	2.54e-48	157
MsG0180004880.01.T01	AT2G30860	38.462	195	119	1	2	195	15	209	2.93e-46	152
MsG0180004880.01.T01	AT2G30870	38.462	195	119	1	2	195	15	209	2.26e-44	147
MsG0180004880.01.T01	AT1G02950	47.887	142	73	1	50	190	60	201	2.84e-44	147
MsG0180004880.01.T01	AT2G30860	36.691	139	88	0	2	140	15	153	1.32e-28	105
MsG0180004880.01.T01	AT1G09640	28.729	181	121	6	17	195	30	204	5.40e-11	61.2
MsG0180004880.01.T01	AT1G09640	28.402	169	114	5	26	193	40	202	9.63e-11	59.7

Find 43 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTAGGGATTAAGACTTTATT+TGG	0.143067	1:-83912312	MsG0180004880.01.T01:CDS
ACTCATTATTGCCATCTTCT+TGG	0.252577	1:+83912395	None:intergenic
GACGGAGATTTAAAGCTCTT+TGG	0.303156	1:-83912680	MsG0180004880.01.T01:intron
GACTTTATTTGGAATTCCTT+TGG	0.310575	1:-83912301	MsG0180004880.01.T01:CDS
TCAATACTTGTTTGGGAACT+AGG	0.337085	1:-83912330	MsG0180004880.01.T01:CDS
AAGTAGCATCAATACTTGTT+TGG	0.355376	1:-83912338	MsG0180004880.01.T01:CDS
GAGGAATGGTGAACACAAAA+AGG	0.363061	1:-83912861	MsG0180004880.01.T01:CDS
AGTAGCATCAATACTTGTTT+GGG	0.373002	1:-83912337	MsG0180004880.01.T01:CDS
ATCTTTATGTTGCAGCCATT+TGG	0.377161	1:-83912722	MsG0180004880.01.T01:intron
GATCATGAGTATGCTGATAA+GGG	0.387666	1:-83912441	MsG0180004880.01.T01:CDS
TGATCATGAGTATGCTGATA+AGG	0.403067	1:-83912442	MsG0180004880.01.T01:CDS
CAAGACCTTATGTTAATGCT+TGG	0.407435	1:-83912104	MsG0180004880.01.T01:CDS
AAGACCTTATGTTAATGCTT+GGG	0.420971	1:-83912103	MsG0180004880.01.T01:CDS
AAATCTCCGTCTTCAAAAGC+TGG	0.444244	1:+83912692	None:intergenic
ATGATCTTAATTAGGTATCA+TGG	0.448138	1:+83912038	None:intergenic
GCTTACATTGATTGATATGA+AGG	0.465911	1:+83912835	None:intergenic
ATGCTACTTTATCGTAGTGT+AGG	0.467657	1:+83912352	None:intergenic
CAATACTTGTTTGGGAACTA+GGG	0.472992	1:-83912329	MsG0180004880.01.T01:CDS
AAAGCTGGAACTTGACCAAA+TGG	0.480424	1:+83912707	None:intergenic
TCCTTTGGATTCAAAAGTTG+TGG	0.481662	1:-83912286	MsG0180004880.01.T01:CDS
AATGAGTGTGTGGTCGGAAG+TGG	0.508607	1:-83912379	MsG0180004880.01.T01:CDS
AGGTGAGTGTTTCACCTTGG+TGG	0.510447	1:-83912190	MsG0180004880.01.T01:CDS
TCAACCCAAGCATTAACATA+AGG	0.524477	1:+83912099	None:intergenic
ATTGTCTGAATCTAAATACA+TGG	0.527540	1:-83912214	MsG0180004880.01.T01:CDS
ATCAAGAATGCTATCCAACT+TGG	0.528639	1:+83912251	None:intergenic
GGAAGAGAATGAAGCCAAGT+TGG	0.538389	1:-83912265	MsG0180004880.01.T01:CDS
AAAATTGAAATGCAGAGTCA+AGG	0.547213	1:-83912482	MsG0180004880.01.T01:intron
AAATTGAAATGCAGAGTCAA+GGG	0.557402	1:-83912481	MsG0180004880.01.T01:intron
TTCATCAAGTAGTGAAGAGA+AGG	0.557567	1:+83912153	None:intergenic
GTAAGCATTAACATGAGGAA+TGG	0.557734	1:-83912875	MsG0180004880.01.T01:CDS
AAGCAGTGACTCCAAGAAGA+TGG	0.558112	1:-83912406	MsG0180004880.01.T01:CDS
GCAAGTACTTTAGACCAAGC+TGG	0.578209	1:+83912060	None:intergenic
CTTACATTGATTGATATGAA+GGG	0.578372	1:+83912836	None:intergenic
GGCAATAATGAGTGTGTGGT+CGG	0.583453	1:-83912385	MsG0180004880.01.T01:CDS
ATATCACTGCAAGACCAGCT+TGG	0.601324	1:-83912074	MsG0180004880.01.T01:CDS
ATCATGAGTATGCTGATAAG+GGG	0.611479	1:-83912440	MsG0180004880.01.T01:CDS
AGTTTGTAAGCATTAACATG+AGG	0.629431	1:-83912880	MsG0180004880.01.T01:CDS
GGGAGGTGAGTGTTTCACCT+TGG	0.632509	1:-83912193	MsG0180004880.01.T01:CDS
TTGTCTGAATCTAAATACAT+GGG	0.650116	1:-83912213	MsG0180004880.01.T01:CDS
CAGAGTTGCAGTAACCCGCA+TGG	0.653094	1:+83912925	None:intergenic
TCTGAATCTAAATACATGGG+AGG	0.678031	1:-83912210	MsG0180004880.01.T01:CDS
TAAATGATGCAAATCCACCA+AGG	0.710594	1:+83912176	None:intergenic
AGATGGCAATAATGAGTGTG+TGG	0.714038	1:-83912389	MsG0180004880.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AACATAAAGATTTAATTAGT+TGG	+	Chr1:83912236-83912255	None:intergenic	15.0%
!	ATTAGTTGGATCAATATAGT+TGG	+	Chr1:83912222-83912241	None:intergenic	25.0%
!	ATTGTCTGAATCTAAATACA+TGG	-	Chr1:83912753-83912772	MsG0180004880.01.T01:intron	25.0%
!	TTGTCTGAATCTAAATACAT+GGG	-	Chr1:83912754-83912773	MsG0180004880.01.T01:intron	25.0%
!!	CTTACATTGATTGATATGAA+GGG	+	Chr1:83912134-83912153	None:intergenic	25.0%
	AAAATTGAAATGCAGAGTCA+AGG	-	Chr1:83912485-83912504	MsG0180004880.01.T01:intron	30.0%
	AAATTGAAATGCAGAGTCAA+GGG	-	Chr1:83912486-83912505	MsG0180004880.01.T01:intron	30.0%
	AAGACCTTATGTTAATGCTT+GGG	-	Chr1:83912864-83912883	MsG0180004880.01.T01:CDS	30.0%
	AAGTAGCATCAATACTTGTT+TGG	-	Chr1:83912629-83912648	MsG0180004880.01.T01:intron	30.0%
	AGTAGCATCAATACTTGTTT+GGG	-	Chr1:83912630-83912649	MsG0180004880.01.T01:intron	30.0%
	AGTTTGTAAGCATTAACATG+AGG	-	Chr1:83912087-83912106	MsG0180004880.01.T01:CDS	30.0%
	CTAGGGATTAAGACTTTATT+TGG	-	Chr1:83912655-83912674	MsG0180004880.01.T01:intron	30.0%
	TCAATATAGTTGGAACAAGA+AGG	+	Chr1:83912212-83912231	None:intergenic	30.0%
!	GACTTTATTTGGAATTCCTT+TGG	-	Chr1:83912666-83912685	MsG0180004880.01.T01:intron	30.0%
!	GCTTACATTGATTGATATGA+AGG	+	Chr1:83912135-83912154	None:intergenic	30.0%
	ACTCATTATTGCCATCTTCT+TGG	+	Chr1:83912575-83912594	None:intergenic	35.0%
	ATCAAGAATGCTATCCAACT+TGG	+	Chr1:83912719-83912738	None:intergenic	35.0%
	ATCATGAGTATGCTGATAAG+GGG	-	Chr1:83912527-83912546	MsG0180004880.01.T01:intron	35.0%
	ATCTTTATGTTGCAGCCATT+TGG	-	Chr1:83912245-83912264	MsG0180004880.01.T01:CDS	35.0%
	CAAGACCTTATGTTAATGCT+TGG	-	Chr1:83912863-83912882	MsG0180004880.01.T01:CDS	35.0%
	CAATACTTGTTTGGGAACTA+GGG	-	Chr1:83912638-83912657	MsG0180004880.01.T01:intron	35.0%
	GATCATGAGTATGCTGATAA+GGG	-	Chr1:83912526-83912545	MsG0180004880.01.T01:intron	35.0%
	GTAAGCATTAACATGAGGAA+TGG	-	Chr1:83912092-83912111	MsG0180004880.01.T01:CDS	35.0%
	TAAATGATGCAAATCCACCA+AGG	+	Chr1:83912794-83912813	None:intergenic	35.0%
	TCAACCCAAGCATTAACATA+AGG	+	Chr1:83912871-83912890	None:intergenic	35.0%
	TCAATACTTGTTTGGGAACT+AGG	-	Chr1:83912637-83912656	MsG0180004880.01.T01:intron	35.0%
	TCCTTTGGATTCAAAAGTTG+TGG	-	Chr1:83912681-83912700	MsG0180004880.01.T01:CDS	35.0%
	TCTGAATCTAAATACATGGG+AGG	-	Chr1:83912757-83912776	MsG0180004880.01.T01:intron	35.0%
	TGATCATGAGTATGCTGATA+AGG	-	Chr1:83912525-83912544	MsG0180004880.01.T01:intron	35.0%
	TTCATCAAGTAGTGAAGAGA+AGG	+	Chr1:83912817-83912836	None:intergenic	35.0%
!	TCCACAACTTTTGAATCCAA+AGG	+	Chr1:83912685-83912704	None:intergenic	35.0%
!!	ATGCTACTTTATCGTAGTGT+AGG	+	Chr1:83912618-83912637	None:intergenic	35.0%
	AAAGCTGGAACTTGACCAAA+TGG	+	Chr1:83912263-83912282	None:intergenic	40.0%
	AAATCTCCGTCTTCAAAAGC+TGG	+	Chr1:83912278-83912297	None:intergenic	40.0%
	AGATGGCAATAATGAGTGTG+TGG	-	Chr1:83912578-83912597	MsG0180004880.01.T01:intron	40.0%
	GAGGAATGGTGAACACAAAA+AGG	-	Chr1:83912106-83912125	MsG0180004880.01.T01:CDS	40.0%
!	GACGGAGATTTAAAGCTCTT+TGG	-	Chr1:83912287-83912306	MsG0180004880.01.T01:CDS	40.0%
!!	CAAGTTCCAGCTTTTGAAGA+CGG	-	Chr1:83912269-83912288	MsG0180004880.01.T01:CDS	40.0%
	AAGCAGTGACTCCAAGAAGA+TGG	-	Chr1:83912561-83912580	MsG0180004880.01.T01:intron	45.0%
	ATATCACTGCAAGACCAGCT+TGG	-	Chr1:83912893-83912912	MsG0180004880.01.T01:CDS	45.0%
	GACACGATATCAAACACACG+TGG	-	Chr1:83912383-83912402	MsG0180004880.01.T01:CDS	45.0%
	GCAAGTACTTTAGACCAAGC+TGG	+	Chr1:83912910-83912929	None:intergenic	45.0%
	GGAAGAGAATGAAGCCAAGT+TGG	-	Chr1:83912702-83912721	MsG0180004880.01.T01:CDS	45.0%
	GGCAATAATGAGTGTGTGGT+CGG	-	Chr1:83912582-83912601	MsG0180004880.01.T01:intron	45.0%
	AATGAGTGTGTGGTCGGAAG+TGG	-	Chr1:83912588-83912607	MsG0180004880.01.T01:intron	50.0%
	AGGTGAGTGTTTCACCTTGG+TGG	-	Chr1:83912777-83912796	MsG0180004880.01.T01:intron	50.0%
	GGGAGGTGAGTGTTTCACCT+TGG	-	Chr1:83912774-83912793	MsG0180004880.01.T01:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	83912044	83912945	83912044	ID=MsG0180004880.01;Name=MsG0180004880.01
Chr1	mRNA	83912044	83912945	83912044	ID=MsG0180004880.01.T01;Parent=MsG0180004880.01;Name=MsG0180004880.01.T01;_AED=0.48;_eAED=0.49;_QI=0\|0\|0\|1\|1\|1\|3\|0\|199
Chr1	exon	83912841	83912945	83912841	ID=MsG0180004880.01.T01:exon:9082;Parent=MsG0180004880.01.T01
Chr1	exon	83912681	83912729	83912681	ID=MsG0180004880.01.T01:exon:9081;Parent=MsG0180004880.01.T01
Chr1	exon	83912044	83912489	83912044	ID=MsG0180004880.01.T01:exon:9080;Parent=MsG0180004880.01.T01
Chr1	CDS	83912841	83912945	83912841	ID=MsG0180004880.01.T01:cds;Parent=MsG0180004880.01.T01
Chr1	CDS	83912681	83912729	83912681	ID=MsG0180004880.01.T01:cds;Parent=MsG0180004880.01.T01
Chr1	CDS	83912044	83912489	83912044	ID=MsG0180004880.01.T01:cds;Parent=MsG0180004880.01.T01

Gene Sequence

>MsG0180004880.01.T01

ATGCGGGTTACTGCAACTCTGTATGAGAAAGAGCTTGAATTTGAGTTTGTAAGCATTAACATGAGGAATGGTGAACACAAAAAGGAACCCTTCATATCAATCAATCCATTTGGTCAAGTTCCAGCTTTTGAAGACGGAGATTTAAAGCTCTTTGAGTCAAGGGCGATAACACAATATATTGATCATGAGTATGCTGATAAGGGGACTAAGTTAACAAGCAGTGACTCCAAGAAGATGGCAATAATGAGTGTGTGGTCGGAAGTGGAAAGCCTACACTACGATAAAGTAGCATCAATACTTGTTTGGGAACTAGGGATTAAGACTTTATTTGGAATTCCTTTGGATTCAAAAGTTGTGGAAGAGAATGAAGCCAAGTTGGATAGCATTCTTGATGTGTATGAGAAAAGATTGTCTGAATCTAAATACATGGGAGGTGAGTGTTTCACCTTGGTGGATTTGCATCATTTACCTTCTCTTCACTACTTGATGAAGTCACAGAGCAAGAAATTGTTTGAATCAAGACCTTATGTTAATGCTTGGGTTGATGATATCACTGCAAGACCAGCTTGGTCTAAAGTACTTGCCATGATACCTAATTAA

Protein sequence

>MsG0180004880.01.T01

MRVTATLYEKELEFEFVSINMRNGEHKKEPFISINPFGQVPAFEDGDLKLFESRAITQYIDHEYADKGTKLTSSDSKKMAIMSVWSEVESLHYDKVASILVWELGIKTLFGIPLDSKVVEENEAKLDSILDVYEKRLSESKYMGGECFTLVDLHHLPSLHYLMKSQSKKLFESRPYVNAWVDDITARPAWSKVLAMIPN*