Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005162.01.T01 | XP_003591832.1 | 98.529 | 340 | 2 | 2 | 1 | 339 | 1 | 338 | 0 | 688 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047814.01 | MsG0180005162.01 | 0.815350 | 9.670052e-52 | 6.650999e-49 |
| MsG0080048856.01 | MsG0180005162.01 | 0.824579 | 7.477120e-54 | 6.650511e-51 |
| MsG0180000144.01 | MsG0180005162.01 | 0.813399 | 2.609945e-51 | 1.702673e-48 |
| MsG0180000555.01 | MsG0180005162.01 | 0.803183 | 3.936108e-49 | 1.965080e-46 |
| MsG0180000836.01 | MsG0180005162.01 | 0.831081 | 2.043222e-55 | 2.193937e-52 |
| MsG0180001173.01 | MsG0180005162.01 | 0.822510 | 2.279083e-53 | 1.911615e-50 |
| MsG0180001566.01 | MsG0180005162.01 | 0.807437 | 5.057356e-50 | 2.816784e-47 |
| MsG0180001662.01 | MsG0180005162.01 | 0.807033 | 6.158145e-50 | 3.394097e-47 |
| MsG0180003031.01 | MsG0180005162.01 | 0.808880 | 2.492193e-50 | 1.441504e-47 |
| MsG0180003304.01 | MsG0180005162.01 | 0.805952 | 1.040924e-49 | 5.580425e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005162.01.T01 | MTR_1g093750 | 98.529 | 340 | 2 | 2 | 1 | 339 | 1 | 338 | 0.0 | 688 |
| MsG0180005162.01.T01 | MTR_3g070970 | 43.119 | 218 | 110 | 5 | 123 | 331 | 42 | 254 | 1.57e-50 | 169 |
| MsG0180005162.01.T01 | MTR_2g015890 | 43.750 | 176 | 85 | 5 | 149 | 317 | 56 | 224 | 7.03e-35 | 128 |
| MsG0180005162.01.T01 | MTR_4g079760 | 40.625 | 192 | 103 | 2 | 155 | 339 | 64 | 251 | 6.55e-31 | 117 |
| MsG0180005162.01.T01 | MTR_2g091190 | 42.458 | 179 | 92 | 3 | 145 | 316 | 45 | 219 | 2.56e-30 | 115 |
| MsG0180005162.01.T01 | MTR_2g015890 | 48.000 | 125 | 57 | 2 | 149 | 272 | 56 | 173 | 6.34e-27 | 106 |
| MsG0180005162.01.T01 | MTR_5g066080 | 34.934 | 229 | 107 | 4 | 136 | 327 | 8 | 231 | 1.64e-26 | 105 |
| MsG0180005162.01.T01 | MTR_4g087920 | 43.407 | 182 | 89 | 6 | 143 | 317 | 62 | 236 | 1.32e-24 | 101 |
| MsG0180005162.01.T01 | MTR_5g032375 | 32.370 | 173 | 93 | 4 | 157 | 316 | 43 | 204 | 6.38e-23 | 95.1 |
| MsG0180005162.01.T01 | MTR_2g015890 | 38.636 | 132 | 68 | 4 | 192 | 317 | 2 | 126 | 1.04e-17 | 79.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005162.01.T01 | AT1G68810 | 64.394 | 264 | 68 | 8 | 96 | 337 | 99 | 358 | 1.24e-93 | 283 |
| MsG0180005162.01.T01 | AT3G25710 | 49.844 | 321 | 96 | 8 | 72 | 333 | 22 | 336 | 5.83e-80 | 248 |
| MsG0180005162.01.T01 | AT3G56770 | 47.059 | 170 | 82 | 4 | 151 | 317 | 37 | 201 | 7.00e-43 | 148 |
| MsG0180005162.01.T01 | AT2G41130 | 46.629 | 178 | 84 | 5 | 143 | 317 | 55 | 224 | 2.57e-38 | 137 |
| MsG0180005162.01.T01 | AT2G40200 | 42.105 | 190 | 89 | 8 | 130 | 311 | 38 | 214 | 1.56e-33 | 124 |
| MsG0180005162.01.T01 | AT4G38070 | 37.324 | 142 | 77 | 3 | 153 | 294 | 87 | 216 | 3.60e-25 | 102 |
| MsG0180005162.01.T01 | AT1G01260 | 31.111 | 180 | 99 | 8 | 38 | 207 | 314 | 478 | 1.67e-11 | 65.9 |
| MsG0180005162.01.T01 | AT1G01260 | 31.111 | 180 | 99 | 8 | 38 | 207 | 314 | 478 | 1.67e-11 | 65.9 |
| MsG0180005162.01.T01 | AT1G01260 | 31.111 | 180 | 99 | 8 | 38 | 207 | 314 | 478 | 1.67e-11 | 65.9 |
| MsG0180005162.01.T01 | AT5G57150 | 25.137 | 183 | 99 | 5 | 166 | 319 | 58 | 231 | 9.03e-11 | 62.0 |
Find 63 sgRNAs with CRISPR-Local
Find 118 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGCTGATATAACAACACT+TGG | 0.602897 | 1:+87831451 | MsG0180005162.01.T01:CDS |
| AAAGGTTCATGAGGGTTGAA+AGG | 0.586522 | 1:-87829782 | None:intergenic |
| AACATGATGATGAGACAATG+AGG | 0.726295 | 1:+87831858 | MsG0180005162.01.T01:three_prime_UTR |
| AATAGTGATCATCATATCTT+TGG | 0.353704 | 1:+87829653 | MsG0180005162.01.T01:CDS |
| AATTAGAATTGATTAAATTA+TGG | 0.270913 | 1:+87831644 | MsG0180005162.01.T01:exon |
| ACAATGAGGTAATGATAATT+TGG | 0.372247 | 1:+87831872 | MsG0180005162.01.T01:three_prime_UTR |
| ACACTTACTTTGGTTGTGCT+TGG | 0.447565 | 1:-87830103 | None:intergenic |
| ACTAATATAATAATAATAAT+AGG | 0.326615 | 1:-87831694 | None:intergenic |
| AGAAGCACTCAAAGCTGTGA+TGG | 0.573378 | 1:+87831546 | MsG0180005162.01.T01:CDS |
| AGAGTCATAGTGAAGCTGAA+AGG | 0.601072 | 1:+87830029 | MsG0180005162.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGCTGATATAACAACACT+TGG | + | Chr1:87831451-87831470 | MsG0180005162.01.T01:CDS | 30.0% | |
| AGAATGTGAAATGGAAATAG+TGG | - | Chr1:87830896-87830915 | None:intergenic | 30.0% | |
| AGTATGATACAAGAAGATCA+AGG | + | Chr1:87829545-87829564 | MsG0180005162.01.T01:exon | 30.0% | |
| ATATCAGATACACTTCAACA+TGG | + | Chr1:87829914-87829933 | MsG0180005162.01.T01:CDS | 30.0% | |
| ATGAGGTAATGATAATTTGG+TGG | + | Chr1:87831875-87831894 | MsG0180005162.01.T01:three_prime_UTR | 30.0% | |
| CTATTGAACAAACTTGCATA+AGG | - | Chr1:87829833-87829852 | None:intergenic | 30.0% | |
| GAATTAGTGTTAATGTCTCT+TGG | + | Chr1:87830669-87830688 | MsG0180005162.01.T01:intron | 30.0% | |
| GATGAAGATTATGGATCAAA+TGG | + | Chr1:87831328-87831347 | MsG0180005162.01.T01:CDS | 30.0% | |
| GTTAATTCATCACATTCAGT+TGG | - | Chr1:87831289-87831308 | None:intergenic | 30.0% | |
| TCATATGCAAATTGAAGAGA+AGG | - | Chr1:87829863-87829882 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 87829525 | 87831905 | 87829525 | ID=MsG0180005162.01;Name=MsG0180005162.01 |
| Chr1 | mRNA | 87829525 | 87831905 | 87829525 | ID=MsG0180005162.01.T01;Parent=MsG0180005162.01;Name=MsG0180005162.01.T01;_AED=0.32;_eAED=0.32;_QI=23|1|1|1|1|1|2|260|339 |
| Chr1 | exon | 87829525 | 87830117 | 87829525 | ID=MsG0180005162.01.T01:exon:14303;Parent=MsG0180005162.01.T01 |
| Chr1 | exon | 87831196 | 87831905 | 87831196 | ID=MsG0180005162.01.T01:exon:14304;Parent=MsG0180005162.01.T01 |
| Chr1 | five_prime_UTR | 87829525 | 87829547 | 87829525 | ID=MsG0180005162.01.T01:five_prime_utr;Parent=MsG0180005162.01.T01 |
| Chr1 | CDS | 87829548 | 87830117 | 87829548 | ID=MsG0180005162.01.T01:cds;Parent=MsG0180005162.01.T01 |
| Chr1 | CDS | 87831196 | 87831645 | 87831196 | ID=MsG0180005162.01.T01:cds;Parent=MsG0180005162.01.T01 |
| Chr1 | three_prime_UTR | 87831646 | 87831905 | 87831646 | ID=MsG0180005162.01.T01:three_prime_utr;Parent=MsG0180005162.01.T01 |
| Gene Sequence |
>MsG0180005162.01.T01 ATGATACAAGAAGATCAAGGACAGTGTTCATCTCAAACAATCAATAACTTTGAAACTTATCAACAAGAGCAGTTTCTCCTTCAACAACAGATGATGAGGCAACAAAATAGTGATCATCATATCTTTGGTGGAGGAAGGTCTATGTTCCCTACAGCAGGAGGAGGGGGTGATCATCAAGTTTCACCAATCTTGCAACAAACATGGTCTATGCAACAACTTCATCATCATCATCATCCTTTCAACCCTCATGAACCTTTCGTTATTCCTCAACAACAAGCTTCACCTTATGCAAGTTTGTTCAATAGAAGGGTTCCTTCTCTTCAATTTGCATATGATCATCATGCAGGTTCTGAGCATCTTAGGATCATATCAGATACACTTCAACATGGATCAGGTTCAGGACCCTTTGGTGGTTTTCAAGGTGAAGTTGGAAAGATGAGTGCTCAAGAAATTATGGAGGCTAAGGCTCTTGCTGCTTCCAAGAGTCATAGTGAAGCTGAAAGGAGACGCAGAGAAAGAATCAATAACCATCTTGCTAAGCTACGCAGCTTGTTGCCAAGCACAACCAAAACAGACAAAGCATCATTGCTAGCAGAAGTGATACAACATGTAAAAGAATTGAAGCGTCAAACATCATTGATAGCAGAAACAAGTCCAGTTCCAACTGAATGTGATGAATTAACAGTAGATGCTGCAGATGATGATGAAGATTATGGATCAAATGGAAACAAGTTTATCATAAAAGCCTCACTTTGCTGTGATGATAGGTCAGATCTTTTGCCTGAACTAATCAAGACATTGAAAGCATTAAGGTTAAGAACACTCAAAGCTGATATAACAACACTTGGAGGACGTGTTAAGAATGTTTTGTTCATTACTGGTGAAGAAGATGATCATGAATATTGCATTAGTTCAATACAAGAAGCACTCAAAGCTGTGATGGAGAAGAGTGTTGGAGATGAATCTGCTTCTGGAAGTGTCAAGAGACAAAGGACTAATATAATATCAATATCTAATTAA |
| Protein sequence |
>MsG0180005162.01.T01 MIQEDQGQCSSQTINNFETYQQEQFLLQQQMMRQQNSDHHIFGGGRSMFPTAGGGGDHQVSPILQQTWSMQQLHHHHHPFNPHEPFVIPQQQASPYASLFNRRVPSLQFAYDHHAGSEHLRIISDTLQHGSGSGPFGGFQGEVGKMSAQEIMEAKALAASKSHSEAERRRRERINNHLAKLRSLLPSTTKTDKASLLAEVIQHVKELKRQTSLIAETSPVPTECDELTVDAADDDEDYGSNGNKFIIKASLCCDDRSDLLPELIKTLKALRLRTLKADITTLGGRVKNVLFITGEEDDHEYCISSIQEALKAVMEKSVGDESASGSVKRQRTNIISISN* |
