Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005162.01.T01 | XP_003591832.1 | 98.529 | 340 | 2 | 2 | 1 | 339 | 1 | 338 | 0 | 688 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005162.01.T01 | Q9S7Y1 | 64.394 | 264 | 68 | 8 | 96 | 337 | 99 | 358 | 1.21E-92 | 283 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005162.01.T01 | G7I4X0 | 98.529 | 340 | 2 | 2 | 1 | 339 | 1 | 338 | 0.0 | 688 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047814.01 | MsG0180005162.01 | 0.815350 | 9.670052e-52 | 6.650999e-49 |
| MsG0080048856.01 | MsG0180005162.01 | 0.824579 | 7.477120e-54 | 6.650511e-51 |
| MsG0180000144.01 | MsG0180005162.01 | 0.813399 | 2.609945e-51 | 1.702673e-48 |
| MsG0180000555.01 | MsG0180005162.01 | 0.803183 | 3.936108e-49 | 1.965080e-46 |
| MsG0180000836.01 | MsG0180005162.01 | 0.831081 | 2.043222e-55 | 2.193937e-52 |
| MsG0180001173.01 | MsG0180005162.01 | 0.822510 | 2.279083e-53 | 1.911615e-50 |
| MsG0180001566.01 | MsG0180005162.01 | 0.807437 | 5.057356e-50 | 2.816784e-47 |
| MsG0180001662.01 | MsG0180005162.01 | 0.807033 | 6.158145e-50 | 3.394097e-47 |
| MsG0180003031.01 | MsG0180005162.01 | 0.808880 | 2.492193e-50 | 1.441504e-47 |
| MsG0180003304.01 | MsG0180005162.01 | 0.805952 | 1.040924e-49 | 5.580425e-47 |
| MsG0180003627.01 | MsG0180005162.01 | 0.806218 | 9.154245e-50 | 4.941062e-47 |
| MsG0180004180.01 | MsG0180005162.01 | 0.811323 | 7.414133e-51 | 4.575307e-48 |
| MsG0180004295.01 | MsG0180005162.01 | 0.820299 | 7.382344e-53 | 5.818705e-50 |
| MsG0180004481.01 | MsG0180005162.01 | 0.815879 | 7.374708e-52 | 5.145977e-49 |
| MsG0180004482.01 | MsG0180005162.01 | 0.810836 | 9.452958e-51 | 5.759273e-48 |
| MsG0180004754.01 | MsG0180005162.01 | 0.811803 | 5.832959e-51 | 3.645502e-48 |
| MsG0180004802.01 | MsG0180005162.01 | 0.816215 | 6.202572e-52 | 4.368525e-49 |
| MsG0180005049.01 | MsG0180005162.01 | 0.831250 | 1.856468e-55 | 2.003190e-52 |
| MsG0180005162.01 | MsG0180005443.01 | 0.823441 | 1.383053e-53 | 1.190908e-50 |
| MsG0180005162.01 | MsG0180005445.01 | 0.842381 | 2.676642e-58 | 4.051511e-55 |
| MsG0180005162.01 | MsG0180005451.01 | 0.806020 | 1.007647e-49 | 5.411325e-47 |
| MsG0180005162.01 | MsG0180005575.01 | 0.827443 | 1.559757e-54 | 1.506143e-51 |
| MsG0180005162.01 | MsG0180005765.01 | 0.802251 | 6.131441e-49 | 2.989221e-46 |
| MsG0180005162.01 | MsG0180005786.01 | 0.801115 | 1.048399e-48 | 4.966291e-46 |
| MsG0180005162.01 | MsG0180006013.01 | 0.808332 | 3.262472e-50 | 1.860316e-47 |
| MsG0180005162.01 | MsG0180006128.01 | 0.804381 | 2.220557e-49 | 1.143249e-46 |
| MsG0180005162.01 | MsG0180006155.01 | 0.821965 | 3.050032e-53 | 2.518536e-50 |
| MsG0180005162.01 | MsG0280006759.01 | 0.802920 | 4.461715e-49 | 2.212576e-46 |
| MsG0180005162.01 | MsG0280007491.01 | 0.812599 | 3.909044e-51 | 2.495995e-48 |
| MsG0180005162.01 | MsG0280007527.01 | 0.814070 | 1.857847e-51 | 1.234240e-48 |
| MsG0180005162.01 | MsG0280007529.01 | 0.816781 | 4.633910e-52 | 3.314542e-49 |
| MsG0180005162.01 | MsG0280007710.01 | 0.814898 | 1.218543e-51 | 8.278000e-49 |
| MsG0180005162.01 | MsG0280008615.01 | 0.805694 | 1.179385e-49 | 6.281142e-47 |
| MsG0180005162.01 | MsG0280008909.01 | 0.829770 | 4.274946e-55 | 4.418476e-52 |
| MsG0180005162.01 | MsG0280009748.01 | 0.805947 | 1.043466e-49 | 5.593279e-47 |
| MsG0180005162.01 | MsG0280009996.01 | 0.800540 | 1.373582e-48 | 6.412342e-46 |
| MsG0180005162.01 | MsG0280010109.01 | 0.817783 | 2.756862e-52 | 2.026812e-49 |
| MsG0180005162.01 | MsG0280010233.01 | 0.815691 | 8.120592e-52 | 5.637841e-49 |
| MsG0180005162.01 | MsG0280010447.01 | 0.806764 | 7.019578e-50 | 3.841975e-47 |
| MsG0180005162.01 | MsG0280010491.01 | 0.826986 | 2.007124e-54 | 1.912289e-51 |
| MsG0180005162.01 | MsG0280010980.01 | 0.803250 | 3.812851e-49 | 1.906790e-46 |
| MsG0180005162.01 | MsG0280011124.01 | 0.817861 | 2.647722e-52 | 1.950705e-49 |
| MsG0180005162.01 | MsG0280011293.01 | 0.816650 | 4.957196e-52 | 3.533098e-49 |
| MsG0180005162.01 | MsG0380013363.01 | 0.813527 | 2.447381e-51 | 1.602165e-48 |
| MsG0180005162.01 | MsG0380013549.01 | 0.815406 | 9.399387e-52 | 6.474611e-49 |
| MsG0180005162.01 | MsG0380013834.01 | 0.824521 | 7.716282e-54 | 6.851249e-51 |
| MsG0180005162.01 | MsG0380014537.01 | 0.804302 | 2.305733e-49 | 1.184707e-46 |
| MsG0180005162.01 | MsG0380015332.01 | 0.805679 | 1.188449e-49 | 6.326724e-47 |
| MsG0180005162.01 | MsG0380015831.01 | 0.802433 | 5.624451e-49 | 2.754824e-46 |
| MsG0180005162.01 | MsG0380015944.01 | 0.800727 | 1.258309e-48 | 5.902079e-46 |
| MsG0180005162.01 | MsG0380016765.01 | 0.812032 | 5.198578e-51 | 3.269067e-48 |
| MsG0180005162.01 | MsG0380016905.01 | 0.806002 | 1.016144e-49 | 5.454688e-47 |
| MsG0180005162.01 | MsG0380016970.01 | 0.810341 | 1.209307e-50 | 7.270818e-48 |
| MsG0180005162.01 | MsG0380016971.01 | 0.834232 | 3.376573e-56 | 3.982089e-53 |
| MsG0180005162.01 | MsG0380017195.01 | 0.809232 | 2.095046e-50 | 1.223369e-47 |
| MsG0180005162.01 | MsG0380017375.01 | 0.830486 | 2.858778e-55 | 3.015975e-52 |
| MsG0180005162.01 | MsG0480018163.01 | 0.812295 | 4.555503e-51 | 2.885426e-48 |
| MsG0180005162.01 | MsG0480018313.01 | 0.829243 | 5.741696e-55 | 5.843445e-52 |
| MsG0180005162.01 | MsG0480018640.01 | 0.802822 | 4.673756e-49 | 2.312135e-46 |
| MsG0180005162.01 | MsG0480019404.01 | 0.802207 | 6.258993e-49 | 3.047869e-46 |
| MsG0180005162.01 | MsG0480019970.01 | 0.804590 | 2.008481e-49 | 1.039626e-46 |
| MsG0180005162.01 | MsG0480020331.01 | 0.811081 | 8.368499e-51 | 5.131630e-48 |
| MsG0180005162.01 | MsG0480020591.01 | 0.802916 | 4.471351e-49 | 2.217140e-46 |
| MsG0180005162.01 | MsG0480020739.01 | 0.817350 | 3.451145e-52 | 2.507222e-49 |
| MsG0180005162.01 | MsG0480020780.01 | 0.808291 | 3.328184e-50 | 1.895696e-47 |
| MsG0180005162.01 | MsG0480022318.01 | 0.817665 | 2.931753e-52 | 2.148371e-49 |
| MsG0180005162.01 | MsG0480022696.01 | 0.805674 | 1.190984e-49 | 6.339338e-47 |
| MsG0180005162.01 | MsG0480023245.01 | 0.801055 | 1.078669e-48 | 5.102057e-46 |
| MsG0180005162.01 | MsG0480023258.01 | 0.808504 | 2.998623e-50 | 1.717514e-47 |
| MsG0180005162.01 | MsG0480023412.01 | 0.820062 | 8.366200e-53 | 6.551688e-50 |
| MsG0180005162.01 | MsG0480023415.01 | 0.801855 | 7.393968e-49 | 3.568663e-46 |
| MsG0180005162.01 | MsG0480023463.01 | 0.807159 | 5.793216e-50 | 3.203326e-47 |
| MsG0180005162.01 | MsG0480023600.01 | 0.830171 | 3.412936e-55 | 3.568233e-52 |
| MsG0180005162.01 | MsG0480023873.01 | 0.853458 | 2.358504e-61 | 5.099601e-58 |
| MsG0180005162.01 | MsG0580024197.01 | 0.820900 | 5.370496e-53 | 4.304549e-50 |
| MsG0180005162.01 | MsG0580024781.01 | 0.811452 | 6.950697e-51 | 4.304207e-48 |
| MsG0180005162.01 | MsG0580024819.01 | 0.811959 | 5.393609e-51 | 3.385075e-48 |
| MsG0180005162.01 | MsG0580025651.01 | 0.803950 | 2.729672e-49 | 1.389973e-46 |
| MsG0180005162.01 | MsG0580027441.01 | 0.807282 | 5.454017e-50 | 3.025607e-47 |
| MsG0180005162.01 | MsG0580029640.01 | 0.800154 | 1.646741e-48 | 7.612303e-46 |
| MsG0180005162.01 | MsG0580029926.01 | 0.817115 | 3.897529e-52 | 2.813330e-49 |
| MsG0180005162.01 | MsG0680031214.01 | 0.818278 | 2.130950e-52 | 1.587974e-49 |
| MsG0180005162.01 | MsG0680031651.01 | 0.803333 | 3.664681e-49 | 1.836587e-46 |
| MsG0180005162.01 | MsG0680031699.01 | 0.804098 | 2.542935e-49 | 1.299748e-46 |
| MsG0180005162.01 | MsG0680032075.01 | 0.811527 | 6.697179e-51 | 4.155453e-48 |
| MsG0180005162.01 | MsG0680033392.01 | 0.803704 | 3.070813e-49 | 1.553667e-46 |
| MsG0180005162.01 | MsG0680035306.01 | 0.816540 | 5.247228e-52 | 3.728330e-49 |
| MsG0180005162.01 | MsG0780036240.01 | 0.803520 | 3.351779e-49 | 1.687969e-46 |
| MsG0180005162.01 | MsG0780036663.01 | 0.814291 | 1.659912e-51 | 1.109404e-48 |
| MsG0180005162.01 | MsG0780036719.01 | 0.809228 | 2.099322e-50 | 1.225723e-47 |
| MsG0180005162.01 | MsG0780036773.01 | 0.817848 | 2.664586e-52 | 1.962484e-49 |
| MsG0180005162.01 | MsG0780037144.01 | 0.800144 | 1.654144e-48 | 7.644866e-46 |
| MsG0180005162.01 | MsG0780038443.01 | 0.808279 | 3.348437e-50 | 1.906671e-47 |
| MsG0180005162.01 | MsG0780038565.01 | 0.816080 | 6.648172e-52 | 4.665177e-49 |
| MsG0180005162.01 | MsG0780039443.01 | 0.805497 | 1.297420e-49 | 6.874275e-47 |
| MsG0180005162.01 | MsG0780040044.01 | 0.811913 | 5.519431e-51 | 3.459625e-48 |
| MsG0180005162.01 | MsG0780040669.01 | 0.816486 | 5.394089e-52 | 3.827040e-49 |
| MsG0180005162.01 | MsG0780041065.01 | 0.803313 | 3.700608e-49 | 1.853603e-46 |
| MsG0180005162.01 | MsG0780041305.01 | 0.837034 | 6.593583e-57 | 8.459956e-54 |
| MsG0180005162.01 | MsG0780041535.01 | 0.800676 | 1.288935e-48 | 6.038036e-46 |
| MsG0180005162.01 | MsG0780041667.01 | 0.816311 | 5.903358e-52 | 4.168812e-49 |
| MsG0180005162.01 | MsG0880042311.01 | 0.817724 | 2.842410e-52 | 2.086382e-49 |
| MsG0180005162.01 | MsG0880042324.01 | 0.804847 | 1.774574e-49 | 9.247458e-47 |
| MsG0180005162.01 | MsG0880042518.01 | 0.815144 | 1.074469e-51 | 7.348095e-49 |
| MsG0180005162.01 | MsG0880042760.01 | 0.841346 | 5.024937e-58 | 7.361819e-55 |
| MsG0180005162.01 | MsG0880042939.01 | 0.808591 | 2.873197e-50 | 1.649341e-47 |
| MsG0180005162.01 | MsG0880043116.01 | 0.812097 | 5.032901e-51 | 3.170667e-48 |
| MsG0180005162.01 | MsG0880043479.01 | 0.804138 | 2.494437e-49 | 1.276263e-46 |
| MsG0180005162.01 | MsG0880043480.01 | 0.816097 | 6.593045e-52 | 4.628544e-49 |
| MsG0180005162.01 | MsG0880043522.01 | 0.804995 | 1.652551e-49 | 8.643994e-47 |
| MsG0180005162.01 | MsG0880043532.01 | 0.819875 | 9.232449e-53 | 7.191786e-50 |
| MsG0180005162.01 | MsG0880043592.01 | 0.812192 | 4.797062e-51 | 3.030015e-48 |
| MsG0180005162.01 | MsG0880044565.01 | 0.811946 | 5.427796e-51 | 3.405409e-48 |
| MsG0180005162.01 | MsG0880045145.01 | 0.802385 | 5.754186e-49 | 2.815027e-46 |
| MsG0180005162.01 | MsG0880045393.01 | 0.813142 | 2.972684e-51 | 1.925604e-48 |
| MsG0180005162.01 | MsG0880045754.01 | 0.821105 | 4.818249e-53 | 3.883962e-50 |
| MsG0180005162.01 | MsG0880046027.01 | 0.847862 | 8.839035e-60 | 1.592422e-56 |
| MsG0180005162.01 | MsG0880047470.01 | 0.830786 | 2.413662e-55 | 2.569180e-52 |
| MsG0180005162.01 | MsG0880047472.01 | 0.810244 | 1.269550e-50 | 7.613603e-48 |
| MsG0180005162.01 | MsG0880047473.01 | 0.837365 | 5.424956e-57 | 7.030853e-54 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005162.01.T01 | MTR_1g093750 | 98.529 | 340 | 2 | 2 | 1 | 339 | 1 | 338 | 0.0 | 688 |
| MsG0180005162.01.T01 | MTR_3g070970 | 43.119 | 218 | 110 | 5 | 123 | 331 | 42 | 254 | 1.57e-50 | 169 |
| MsG0180005162.01.T01 | MTR_2g015890 | 43.750 | 176 | 85 | 5 | 149 | 317 | 56 | 224 | 7.03e-35 | 128 |
| MsG0180005162.01.T01 | MTR_4g079760 | 40.625 | 192 | 103 | 2 | 155 | 339 | 64 | 251 | 6.55e-31 | 117 |
| MsG0180005162.01.T01 | MTR_2g091190 | 42.458 | 179 | 92 | 3 | 145 | 316 | 45 | 219 | 2.56e-30 | 115 |
| MsG0180005162.01.T01 | MTR_2g015890 | 48.000 | 125 | 57 | 2 | 149 | 272 | 56 | 173 | 6.34e-27 | 106 |
| MsG0180005162.01.T01 | MTR_5g066080 | 34.934 | 229 | 107 | 4 | 136 | 327 | 8 | 231 | 1.64e-26 | 105 |
| MsG0180005162.01.T01 | MTR_4g087920 | 43.407 | 182 | 89 | 6 | 143 | 317 | 62 | 236 | 1.32e-24 | 101 |
| MsG0180005162.01.T01 | MTR_5g032375 | 32.370 | 173 | 93 | 4 | 157 | 316 | 43 | 204 | 6.38e-23 | 95.1 |
| MsG0180005162.01.T01 | MTR_2g015890 | 38.636 | 132 | 68 | 4 | 192 | 317 | 2 | 126 | 1.04e-17 | 79.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005162.01.T01 | AT1G68810 | 64.394 | 264 | 68 | 8 | 96 | 337 | 99 | 358 | 1.24e-93 | 283 |
| MsG0180005162.01.T01 | AT3G25710 | 49.844 | 321 | 96 | 8 | 72 | 333 | 22 | 336 | 5.83e-80 | 248 |
| MsG0180005162.01.T01 | AT3G56770 | 47.059 | 170 | 82 | 4 | 151 | 317 | 37 | 201 | 7.00e-43 | 148 |
| MsG0180005162.01.T01 | AT2G41130 | 46.629 | 178 | 84 | 5 | 143 | 317 | 55 | 224 | 2.57e-38 | 137 |
| MsG0180005162.01.T01 | AT2G40200 | 42.105 | 190 | 89 | 8 | 130 | 311 | 38 | 214 | 1.56e-33 | 124 |
| MsG0180005162.01.T01 | AT4G38070 | 37.324 | 142 | 77 | 3 | 153 | 294 | 87 | 216 | 3.60e-25 | 102 |
| MsG0180005162.01.T01 | AT1G01260 | 31.111 | 180 | 99 | 8 | 38 | 207 | 314 | 478 | 1.67e-11 | 65.9 |
| MsG0180005162.01.T01 | AT1G01260 | 31.111 | 180 | 99 | 8 | 38 | 207 | 314 | 478 | 1.67e-11 | 65.9 |
| MsG0180005162.01.T01 | AT1G01260 | 31.111 | 180 | 99 | 8 | 38 | 207 | 314 | 478 | 1.67e-11 | 65.9 |
| MsG0180005162.01.T01 | AT5G57150 | 25.137 | 183 | 99 | 5 | 166 | 319 | 58 | 231 | 9.03e-11 | 62.0 |
Find 63 sgRNAs with CRISPR-Local
Find 118 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTATTATTATATTAGTATA+TGG | 0.263249 | 1:+87831700 | MsG0180005162.01.T01:three_prime_UTR |
| AATTAGAATTGATTAAATTA+TGG | 0.270913 | 1:+87831644 | MsG0180005162.01.T01:exon |
| ATGCAAGTTTGTTCAATAGA+AGG | 0.283417 | 1:+87829834 | MsG0180005162.01.T01:CDS |
| GATGAGTGCTCAAGAAATTA+TGG | 0.286315 | 1:+87829982 | MsG0180005162.01.T01:CDS |
| TCAAGACATTGAAAGCATTA+AGG | 0.296728 | 1:+87831416 | MsG0180005162.01.T01:CDS |
| TTCAATGTCTTGATTAGTTC+AGG | 0.303436 | 1:-87831406 | None:intergenic |
| ATGCAGGTTCTGAGCATCTT+AGG | 0.326044 | 1:+87829888 | MsG0180005162.01.T01:CDS |
| ACTAATATAATAATAATAAT+AGG | 0.326615 | 1:-87831694 | None:intergenic |
| GCAGATGATGATGAAGATTA+TGG | 0.331671 | 1:+87831319 | MsG0180005162.01.T01:CDS |
| GTTGGAGATGAATCTGCTTC+TGG | 0.334849 | 1:+87831577 | MsG0180005162.01.T01:CDS |
| AATAGTGATCATCATATCTT+TGG | 0.353704 | 1:+87829653 | MsG0180005162.01.T01:CDS |
| TGATCTTCTTGTATCATACT+TGG | 0.359086 | 1:-87829542 | None:intergenic |
| GTAAGATGTTGTTATGTATA+TGG | 0.364483 | 1:+87831827 | MsG0180005162.01.T01:three_prime_UTR |
| ACAATGAGGTAATGATAATT+TGG | 0.372247 | 1:+87831872 | MsG0180005162.01.T01:three_prime_UTR |
| GACCATGTTTGTTGCAAGAT+TGG | 0.409233 | 1:-87829731 | None:intergenic |
| GATGAAGATTATGGATCAAA+TGG | 0.416803 | 1:+87831328 | MsG0180005162.01.T01:CDS |
| TTCAGCTTCACTATGACTCT+TGG | 0.417321 | 1:-87830026 | None:intergenic |
| TTATATTAGTATATGGTACT+TGG | 0.421823 | 1:+87831707 | MsG0180005162.01.T01:three_prime_UTR |
| TCATCACATTCAGTTGGAAC+TGG | 0.424698 | 1:-87831280 | None:intergenic |
| CCTCACTTTGCTGTGATGAT+AGG | 0.438078 | 1:+87831371 | MsG0180005162.01.T01:CDS |
| GGATCAGGTTCAGGACCCTT+TGG | 0.439350 | 1:+87829935 | MsG0180005162.01.T01:CDS |
| ACACTTACTTTGGTTGTGCT+TGG | 0.447565 | 1:-87830103 | None:intergenic |
| CTATTGAACAAACTTGCATA+AGG | 0.450006 | 1:-87829830 | None:intergenic |
| CTTCAACATGGATCAGGTTC+AGG | 0.466813 | 1:+87829926 | MsG0180005162.01.T01:CDS |
| GCCTCATCATCTGTTGTTGA+AGG | 0.470688 | 1:-87829625 | None:intergenic |
| TGCAAGTTTGTTCAATAGAA+GGG | 0.484018 | 1:+87829835 | MsG0180005162.01.T01:CDS |
| GTTAATTCATCACATTCAGT+TGG | 0.495856 | 1:-87831286 | None:intergenic |
| TTCCCTACAGCAGGAGGAGG+GGG | 0.504915 | 1:+87829692 | MsG0180005162.01.T01:CDS |
| ATGAGGTAATGATAATTTGG+TGG | 0.510319 | 1:+87831875 | MsG0180005162.01.T01:three_prime_UTR |
| TCAGGTTCAGGACCCTTTGG+TGG | 0.514868 | 1:+87829938 | MsG0180005162.01.T01:CDS |
| AGTGATCATCATATCTTTGG+TGG | 0.524209 | 1:+87829656 | MsG0180005162.01.T01:CDS |
| ATGTTCCCTACAGCAGGAGG+AGG | 0.528325 | 1:+87829689 | MsG0180005162.01.T01:CDS |
| AGGTCTATGTTCCCTACAGC+AGG | 0.528893 | 1:+87829683 | MsG0180005162.01.T01:CDS |
| GCTGATATAACAACACTTGG+AGG | 0.532615 | 1:+87831454 | MsG0180005162.01.T01:CDS |
| CACCAATCTTGCAACAAACA+TGG | 0.533876 | 1:+87829729 | MsG0180005162.01.T01:CDS |
| TTTGCATATGATCATCATGC+AGG | 0.539983 | 1:+87829872 | MsG0180005162.01.T01:CDS |
| TCAAGAAATTATGGAGGCTA+AGG | 0.546278 | 1:+87829991 | MsG0180005162.01.T01:CDS |
| GATACACTTCAACATGGATC+AGG | 0.552437 | 1:+87829920 | MsG0180005162.01.T01:CDS |
| TCATATGCAAATTGAAGAGA+AGG | 0.553108 | 1:-87829860 | None:intergenic |
| ATCATATCTTTGGTGGAGGA+AGG | 0.562826 | 1:+87829663 | MsG0180005162.01.T01:CDS |
| TGTTCCCTACAGCAGGAGGA+GGG | 0.570414 | 1:+87829690 | MsG0180005162.01.T01:CDS |
| AGAAGCACTCAAAGCTGTGA+TGG | 0.573378 | 1:+87831546 | MsG0180005162.01.T01:CDS |
| AGCTGCGTAGCTTAGCAAGA+TGG | 0.576612 | 1:-87830075 | None:intergenic |
| TCCTTCAACAACAGATGATG+AGG | 0.577140 | 1:+87829624 | MsG0180005162.01.T01:CDS |
| TGTTGTTGAGGAATAACGAA+AGG | 0.577534 | 1:-87829800 | None:intergenic |
| GATCATCATATCTTTGGTGG+AGG | 0.579767 | 1:+87829659 | MsG0180005162.01.T01:CDS |
| TTCACCTTGAAAACCACCAA+AGG | 0.584286 | 1:-87829951 | None:intergenic |
| GTTCCCTACAGCAGGAGGAG+GGG | 0.584625 | 1:+87829691 | MsG0180005162.01.T01:CDS |
| TCACCTTGAAAACCACCAAA+GGG | 0.586225 | 1:-87829950 | None:intergenic |
| AAAGGTTCATGAGGGTTGAA+AGG | 0.586522 | 1:-87829782 | None:intergenic |
| TAAGGTGAAGCTTGTTGTTG+AGG | 0.593376 | 1:-87829812 | None:intergenic |
| AGAGTCATAGTGAAGCTGAA+AGG | 0.601072 | 1:+87830029 | MsG0180005162.01.T01:CDS |
| AAAGCTGATATAACAACACT+TGG | 0.602897 | 1:+87831451 | MsG0180005162.01.T01:CDS |
| CTGGAAGTGTCAAGAGACAA+AGG | 0.603991 | 1:+87831596 | MsG0180005162.01.T01:CDS |
| GCTGTGATGGAGAAGAGTGT+TGG | 0.619796 | 1:+87831559 | MsG0180005162.01.T01:CDS |
| CCTATCATCACAGCAAAGTG+AGG | 0.634985 | 1:-87831371 | None:intergenic |
| AGTATGATACAAGAAGATCA+AGG | 0.636259 | 1:+87829545 | MsG0180005162.01.T01:exon |
| GGAATAACGAAAGGTTCATG+AGG | 0.639813 | 1:-87829791 | None:intergenic |
| TCTATGTTCCCTACAGCAGG+AGG | 0.647545 | 1:+87829686 | MsG0180005162.01.T01:CDS |
| GAATAACGAAAGGTTCATGA+GGG | 0.671930 | 1:-87829790 | None:intergenic |
| ATATCAGATACACTTCAACA+TGG | 0.699194 | 1:+87829914 | MsG0180005162.01.T01:CDS |
| GAGTGCTCAAGAAATTATGG+AGG | 0.703562 | 1:+87829985 | MsG0180005162.01.T01:CDS |
| AACATGATGATGAGACAATG+AGG | 0.726295 | 1:+87831858 | MsG0180005162.01.T01:three_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTTATAGTTAATTAAAG+TGG | - | Chr1:87831094-87831113 | None:intergenic | 10.0% |
| !! | AATTAGAATTGATTAAATTA+TGG | + | Chr1:87831644-87831663 | MsG0180005162.01.T01:exon | 10.0% |
| !! | ATTATATATTAGTACTATAT+TGG | - | Chr1:87830268-87830287 | None:intergenic | 10.0% |
| !!! | TTTTTATCTTTTTTTTTTTG+GGG | + | Chr1:87831762-87831781 | MsG0180005162.01.T01:three_prime_UTR | 10.0% |
| !!! | TTTTATCTTTTTTTTTTTGG+GGG | + | Chr1:87831763-87831782 | MsG0180005162.01.T01:three_prime_UTR | 15.0% |
| !! | ATAGATCTGATATATACATA+AGG | - | Chr1:87830946-87830965 | None:intergenic | 20.0% |
| !! | TATGATATAAGAATGTGAAA+TGG | - | Chr1:87830905-87830924 | None:intergenic | 20.0% |
| !! | TGAAAATATTTACAATCTCA+TGG | - | Chr1:87830979-87830998 | None:intergenic | 20.0% |
| !! | TTATATTAGTATATGGTACT+TGG | + | Chr1:87831707-87831726 | MsG0180005162.01.T01:three_prime_UTR | 20.0% |
| !!! | AATAGTGGATATATTTTGAA+TGG | - | Chr1:87830881-87830900 | None:intergenic | 20.0% |
| !!! | AGATATTTTGAATCTACAAA+AGG | - | Chr1:87830379-87830398 | None:intergenic | 20.0% |
| !!! | GATTTTGATTTTGTTGTTTA+TGG | - | Chr1:87830414-87830433 | None:intergenic | 20.0% |
| !!! | TCTTTTCTTTTCTTTTTTCA+TGG | + | Chr1:87831798-87831817 | MsG0180005162.01.T01:three_prime_UTR | 20.0% |
| ! | AATAGTGATCATCATATCTT+TGG | + | Chr1:87829653-87829672 | MsG0180005162.01.T01:CDS | 25.0% |
| ! | AATATTTACAATCTCATGGT+AGG | - | Chr1:87830975-87830994 | None:intergenic | 25.0% |
| ! | ACAATGAGGTAATGATAATT+TGG | + | Chr1:87831872-87831891 | MsG0180005162.01.T01:three_prime_UTR | 25.0% |
| ! | ATATTTACAATCTCATGGTA+GGG | - | Chr1:87830974-87830993 | None:intergenic | 25.0% |
| ! | ATCTGATATATACATAAGGT+TGG | - | Chr1:87830942-87830961 | None:intergenic | 25.0% |
| ! | GGCATATATTGAATTCAAAA+AGG | - | Chr1:87830358-87830377 | None:intergenic | 25.0% |
| ! | GTAAGATGTTGTTATGTATA+TGG | + | Chr1:87831827-87831846 | MsG0180005162.01.T01:three_prime_UTR | 25.0% |
| ! | TGAAAATATATTGTCTTTGC+CGG | + | Chr1:87830738-87830757 | MsG0180005162.01.T01:intron | 25.0% |
| ! | TGGAGAATGAAAAGAAATAA+AGG | + | Chr1:87830788-87830807 | MsG0180005162.01.T01:intron | 25.0% |
| ! | TTACAACTATACACTTACTT+TGG | - | Chr1:87830116-87830135 | None:intergenic | 25.0% |
| !! | GGTTTGCATTTTAAATTTCT+TGG | - | Chr1:87830224-87830243 | None:intergenic | 25.0% |
| !!! | AAGAATGTTTTGTTCATTAC+TGG | + | Chr1:87831484-87831503 | MsG0180005162.01.T01:CDS | 25.0% |
| !!! | CACATTGTTCTCTTTTTTTT+TGG | + | Chr1:87830521-87830540 | MsG0180005162.01.T01:intron | 25.0% |
| !!! | GATAATTTGGTGGTTTTATT+TGG | + | Chr1:87831885-87831904 | MsG0180005162.01.T01:three_prime_UTR | 25.0% |
| !!! | GTAACTATATGTTTTGTTGT+GGG | + | Chr1:87830574-87830593 | MsG0180005162.01.T01:intron | 25.0% |
| !!! | TAGTAACCTTTTAGAAAATG+AGG | + | Chr1:87830437-87830456 | MsG0180005162.01.T01:intron | 25.0% |
| !!! | TGTAACTATATGTTTTGTTG+TGG | + | Chr1:87830573-87830592 | MsG0180005162.01.T01:intron | 25.0% |
| AAAGCTGATATAACAACACT+TGG | + | Chr1:87831451-87831470 | MsG0180005162.01.T01:CDS | 30.0% | |
| AGAATGTGAAATGGAAATAG+TGG | - | Chr1:87830896-87830915 | None:intergenic | 30.0% | |
| AGTATGATACAAGAAGATCA+AGG | + | Chr1:87829545-87829564 | MsG0180005162.01.T01:exon | 30.0% | |
| ATATCAGATACACTTCAACA+TGG | + | Chr1:87829914-87829933 | MsG0180005162.01.T01:CDS | 30.0% | |
| ATGAGGTAATGATAATTTGG+TGG | + | Chr1:87831875-87831894 | MsG0180005162.01.T01:three_prime_UTR | 30.0% | |
| CTATTGAACAAACTTGCATA+AGG | - | Chr1:87829833-87829852 | None:intergenic | 30.0% | |
| GAATTAGTGTTAATGTCTCT+TGG | + | Chr1:87830669-87830688 | MsG0180005162.01.T01:intron | 30.0% | |
| GATGAAGATTATGGATCAAA+TGG | + | Chr1:87831328-87831347 | MsG0180005162.01.T01:CDS | 30.0% | |
| GTTAATTCATCACATTCAGT+TGG | - | Chr1:87831289-87831308 | None:intergenic | 30.0% | |
| TCATATGCAAATTGAAGAGA+AGG | - | Chr1:87829863-87829882 | None:intergenic | 30.0% | |
| TGACTGATCAACATATTATC+CGG | - | Chr1:87830760-87830779 | None:intergenic | 30.0% | |
| TGATCTTCTTGTATCATACT+TGG | - | Chr1:87829545-87829564 | None:intergenic | 30.0% | |
| ! | ATGCAAGTTTGTTCAATAGA+AGG | + | Chr1:87829834-87829853 | MsG0180005162.01.T01:CDS | 30.0% |
| ! | GATATATACATAAGGTTGGT+AGG | - | Chr1:87830938-87830957 | None:intergenic | 30.0% |
| ! | GCTTTTGTTAACATCCTTTT+TGG | + | Chr1:87830485-87830504 | MsG0180005162.01.T01:intron | 30.0% |
| ! | TGCAAGTTTGTTCAATAGAA+GGG | + | Chr1:87829835-87829854 | MsG0180005162.01.T01:CDS | 30.0% |
| ! | TTCAATGTCTTGATTAGTTC+AGG | - | Chr1:87831409-87831428 | None:intergenic | 30.0% |
| ! | TTTGCACCTCATTTTCTAAA+AGG | - | Chr1:87830446-87830465 | None:intergenic | 30.0% |
| !! | AGTGGATATATTTTGAATGG+TGG | - | Chr1:87830878-87830897 | None:intergenic | 30.0% |
| !! | TCAAGACATTGAAAGCATTA+AGG | + | Chr1:87831416-87831435 | MsG0180005162.01.T01:CDS | 30.0% |
| !! | TTGAGTTGAGATCTTTGATT+AGG | - | Chr1:87830245-87830264 | None:intergenic | 30.0% |
| !!! | TATCTTTTTTTTTTTGGGGG+TGG | + | Chr1:87831766-87831785 | MsG0180005162.01.T01:three_prime_UTR | 30.0% |
| AGTGATCATCATATCTTTGG+TGG | + | Chr1:87829656-87829675 | MsG0180005162.01.T01:CDS | 35.0% | |
| GAATAACGAAAGGTTCATGA+GGG | - | Chr1:87829793-87829812 | None:intergenic | 35.0% | |
| GATGAGTGCTCAAGAAATTA+TGG | + | Chr1:87829982-87830001 | MsG0180005162.01.T01:CDS | 35.0% | |
| GTTATGTTTGTGACTGACAA+AGG | - | Chr1:87830832-87830851 | None:intergenic | 35.0% | |
| GTTGATCAGTCAAACTCAAT+TGG | + | Chr1:87830768-87830787 | MsG0180005162.01.T01:intron | 35.0% | |
| TATAATGTGTGGTAGTTCCA+AGG | + | Chr1:87830605-87830624 | MsG0180005162.01.T01:intron | 35.0% | |
| TCAAGAAATTATGGAGGCTA+AGG | + | Chr1:87829991-87830010 | MsG0180005162.01.T01:CDS | 35.0% | |
| TGTCTCTTGGTATGATAAAG+AGG | + | Chr1:87830682-87830701 | MsG0180005162.01.T01:intron | 35.0% | |
| TGTTGTTGAGGAATAACGAA+AGG | - | Chr1:87829803-87829822 | None:intergenic | 35.0% | |
| TTTGCATATGATCATCATGC+AGG | + | Chr1:87829872-87829891 | MsG0180005162.01.T01:CDS | 35.0% | |
| ! | AACATGATGATGAGACAATG+AGG | + | Chr1:87831858-87831877 | MsG0180005162.01.T01:three_prime_UTR | 35.0% |
| ! | GCAGATGATGATGAAGATTA+TGG | + | Chr1:87831319-87831338 | MsG0180005162.01.T01:CDS | 35.0% |
| !! | TTTTCAACTGCATGCTGTTT+TGG | - | Chr1:87830710-87830729 | None:intergenic | 35.0% |
| !!! | GCAAGAGTGTTTTTTCTAAG+TGG | + | Chr1:87830332-87830351 | MsG0180005162.01.T01:intron | 35.0% |
| AAAGGTTCATGAGGGTTGAA+AGG | - | Chr1:87829785-87829804 | None:intergenic | 40.0% | |
| ACACTTACTTTGGTTGTGCT+TGG | - | Chr1:87830106-87830125 | None:intergenic | 40.0% | |
| AGAGTCATAGTGAAGCTGAA+AGG | + | Chr1:87830029-87830048 | MsG0180005162.01.T01:CDS | 40.0% | |
| ATCATATCTTTGGTGGAGGA+AGG | + | Chr1:87829663-87829682 | MsG0180005162.01.T01:CDS | 40.0% | |
| CACCAATCTTGCAACAAACA+TGG | + | Chr1:87829729-87829748 | MsG0180005162.01.T01:CDS | 40.0% | |
| CTAGCTGGCTAAAGAAAAAG+TGG | + | Chr1:87830634-87830653 | MsG0180005162.01.T01:intron | 40.0% | |
| GAGTGCTCAAGAAATTATGG+AGG | + | Chr1:87829985-87830004 | MsG0180005162.01.T01:CDS | 40.0% | |
| GATACACTTCAACATGGATC+AGG | + | Chr1:87829920-87829939 | MsG0180005162.01.T01:CDS | 40.0% | |
| GATCATCATATCTTTGGTGG+AGG | + | Chr1:87829659-87829678 | MsG0180005162.01.T01:CDS | 40.0% | |
| GCTGATATAACAACACTTGG+AGG | + | Chr1:87831454-87831473 | MsG0180005162.01.T01:CDS | 40.0% | |
| GGAATAACGAAAGGTTCATG+AGG | - | Chr1:87829794-87829813 | None:intergenic | 40.0% | |
| GTATACCAGACAAGCCAAAA+AGG | - | Chr1:87830502-87830521 | None:intergenic | 40.0% | |
| TCACCTTGAAAACCACCAAA+GGG | - | Chr1:87829953-87829972 | None:intergenic | 40.0% | |
| TCATCACATTCAGTTGGAAC+TGG | - | Chr1:87831283-87831302 | None:intergenic | 40.0% | |
| TCCTTCAACAACAGATGATG+AGG | + | Chr1:87829624-87829643 | MsG0180005162.01.T01:CDS | 40.0% | |
| TTCACCTTGAAAACCACCAA+AGG | - | Chr1:87829954-87829973 | None:intergenic | 40.0% | |
| TTCAGCTTCACTATGACTCT+TGG | - | Chr1:87830029-87830048 | None:intergenic | 40.0% | |
| ! | GACCATGTTTGTTGCAAGAT+TGG | - | Chr1:87829734-87829753 | None:intergenic | 40.0% |
| !! | TAAGGTGAAGCTTGTTGTTG+AGG | - | Chr1:87829815-87829834 | None:intergenic | 40.0% |
| !!! | AACATCCTTTTTGGCTTGTC+TGG | + | Chr1:87830494-87830513 | MsG0180005162.01.T01:intron | 40.0% |
| CCTATCATCACAGCAAAGTG+AGG | - | Chr1:87831374-87831393 | None:intergenic | 45.0% | |
| CTTCAACATGGATCAGGTTC+AGG | + | Chr1:87829926-87829945 | MsG0180005162.01.T01:CDS | 45.0% | |
| GCCTCATCATCTGTTGTTGA+AGG | - | Chr1:87829628-87829647 | None:intergenic | 45.0% | |
| GTTGGAGATGAATCTGCTTC+TGG | + | Chr1:87831577-87831596 | MsG0180005162.01.T01:CDS | 45.0% | |
| ! | CCTCACTTTGCTGTGATGAT+AGG | + | Chr1:87831371-87831390 | MsG0180005162.01.T01:CDS | 45.0% |
| !! | AGAAGCACTCAAAGCTGTGA+TGG | + | Chr1:87831546-87831565 | MsG0180005162.01.T01:CDS | 45.0% |
| !! | ATGCAGGTTCTGAGCATCTT+AGG | + | Chr1:87829888-87829907 | MsG0180005162.01.T01:CDS | 45.0% |
| !! | CTGGAAGTGTCAAGAGACAA+AGG | + | Chr1:87831596-87831615 | MsG0180005162.01.T01:CDS | 45.0% |
| !! | GGGAGTGTCTCTATAATGTG+TGG | + | Chr1:87830594-87830613 | MsG0180005162.01.T01:intron | 45.0% |
| !! | GGTGGTTTTCAAGGTGAAGT+TGG | + | Chr1:87829956-87829975 | MsG0180005162.01.T01:CDS | 45.0% |
| !! | ACTAATATAATAATAATAAT+AGG | - | Chr1:87831697-87831716 | None:intergenic | 5.0% |
| !! | ATTATTATTATATTAGTATA+TGG | + | Chr1:87831700-87831719 | MsG0180005162.01.T01:three_prime_UTR | 5.0% |
| !!! | AATTTTTATCTTTTTTTTTT+TGG | + | Chr1:87831760-87831779 | MsG0180005162.01.T01:three_prime_UTR | 5.0% |
| !!! | ATTTTTATCTTTTTTTTTTT+GGG | + | Chr1:87831761-87831780 | MsG0180005162.01.T01:three_prime_UTR | 5.0% |
| !!! | GTTATTATATATTTTTTATT+TGG | + | Chr1:87831729-87831748 | MsG0180005162.01.T01:three_prime_UTR | 5.0% |
| !!! | TATAAATTTTATCTAATTAA+AGG | + | Chr1:87831671-87831690 | MsG0180005162.01.T01:three_prime_UTR | 5.0% |
| AGCTGCGTAGCTTAGCAAGA+TGG | - | Chr1:87830078-87830097 | None:intergenic | 50.0% | |
| AGGTCTATGTTCCCTACAGC+AGG | + | Chr1:87829683-87829702 | MsG0180005162.01.T01:CDS | 50.0% | |
| TCTATGTTCCCTACAGCAGG+AGG | + | Chr1:87829686-87829705 | MsG0180005162.01.T01:CDS | 50.0% | |
| TGAGCTGAGCTGAGATCTCA+TGG | - | Chr1:87831005-87831024 | None:intergenic | 50.0% | |
| ! | GCTGTGATGGAGAAGAGTGT+TGG | + | Chr1:87831559-87831578 | MsG0180005162.01.T01:CDS | 50.0% |
| !! | GGACCCTTTGGTGGTTTTCA+AGG | + | Chr1:87829947-87829966 | MsG0180005162.01.T01:CDS | 50.0% |
| ATGTTCCCTACAGCAGGAGG+AGG | + | Chr1:87829689-87829708 | MsG0180005162.01.T01:CDS | 55.0% | |
| GGATCAGGTTCAGGACCCTT+TGG | + | Chr1:87829935-87829954 | MsG0180005162.01.T01:CDS | 55.0% | |
| GTTCCAAGGAAGCAGCTAGC+TGG | + | Chr1:87830619-87830638 | MsG0180005162.01.T01:intron | 55.0% | |
| TAGCCAGCTAGCTGCTTCCT+TGG | - | Chr1:87830625-87830644 | None:intergenic | 55.0% | |
| TCAGGTTCAGGACCCTTTGG+TGG | + | Chr1:87829938-87829957 | MsG0180005162.01.T01:CDS | 55.0% | |
| TGTTCCCTACAGCAGGAGGA+GGG | + | Chr1:87829690-87829709 | MsG0180005162.01.T01:CDS | 55.0% | |
| GTTCCCTACAGCAGGAGGAG+GGG | + | Chr1:87829691-87829710 | MsG0180005162.01.T01:CDS | 60.0% | |
| TTCCCTACAGCAGGAGGAGG+GGG | + | Chr1:87829692-87829711 | MsG0180005162.01.T01:CDS | 60.0% | |
| CACCCCCTCCTCCTGCTGTA+GGG | - | Chr1:87829697-87829716 | None:intergenic | 65.0% | |
| TCACCCCCTCCTCCTGCTGT+AGG | - | Chr1:87829698-87829717 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 87829525 | 87831905 | 87829525 | ID=MsG0180005162.01;Name=MsG0180005162.01 |
| Chr1 | mRNA | 87829525 | 87831905 | 87829525 | ID=MsG0180005162.01.T01;Parent=MsG0180005162.01;Name=MsG0180005162.01.T01;_AED=0.32;_eAED=0.32;_QI=23|1|1|1|1|1|2|260|339 |
| Chr1 | exon | 87829525 | 87830117 | 87829525 | ID=MsG0180005162.01.T01:exon:14303;Parent=MsG0180005162.01.T01 |
| Chr1 | exon | 87831196 | 87831905 | 87831196 | ID=MsG0180005162.01.T01:exon:14304;Parent=MsG0180005162.01.T01 |
| Chr1 | five_prime_UTR | 87829525 | 87829547 | 87829525 | ID=MsG0180005162.01.T01:five_prime_utr;Parent=MsG0180005162.01.T01 |
| Chr1 | CDS | 87829548 | 87830117 | 87829548 | ID=MsG0180005162.01.T01:cds;Parent=MsG0180005162.01.T01 |
| Chr1 | CDS | 87831196 | 87831645 | 87831196 | ID=MsG0180005162.01.T01:cds;Parent=MsG0180005162.01.T01 |
| Chr1 | three_prime_UTR | 87831646 | 87831905 | 87831646 | ID=MsG0180005162.01.T01:three_prime_utr;Parent=MsG0180005162.01.T01 |
| Gene Sequence |
| Protein sequence |
