AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005262.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005262.01.T01 MTR_1g095830 89.222 167 5 1 1 154 1 167 7.26e-106 301
MsG0180005262.01.T01 MTR_1g095830 100.000 119 0 0 1 119 1 119 3.55e-85 247
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005262.01.T01 AT3G52300 73.054 167 32 1 1 154 1 167 4.24e-87 253
MsG0180005262.01.T01 AT3G52300 78.704 108 23 0 1 108 1 108 1.59e-61 187

Find 44 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTATAATATTCCCAGTCTA+TGG 0.167189 1:+89301621 None:intergenic
CAGTCAAGACACCCAATTTC+AGG 0.179551 1:-89299095 MsG0180005262.01.T01:three_prime_UTR
CTTACCAGTGCATCAAATTT+AGG 0.208576 1:+89299648 None:intergenic
GTATACATTTGTTCTGATTC+TGG 0.223703 1:-89299024 MsG0180005262.01.T01:three_prime_UTR
ATTTAGAAACTGTTGTCTTA+TGG 0.283362 1:-89299121 MsG0180005262.01.T01:three_prime_UTR
AGGAGATTAGGAACGATAAC+TGG 0.290354 1:-89299307 MsG0180005262.01.T01:CDS
GCTGAAGTTGGTTGAGCTTA+AGG 0.315735 1:-89299548 MsG0180005262.01.T01:intron
GTCCCAATCGATCTTCTTCC+CGG 0.344356 1:+89302475 None:intergenic
AATAAATTGTTGTGTTTGTC+TGG 0.357169 1:-89299050 MsG0180005262.01.T01:three_prime_UTR
AAAAGGAATTGGCACTCGTT+TGG 0.433018 1:-89301599 MsG0180005262.01.T01:CDS
AGCAATTTGATGAGGAGATT+AGG 0.438102 1:-89299319 MsG0180005262.01.T01:CDS
TCAAATTGCTTCTTCAGCTC+AGG 0.438466 1:+89299332 None:intergenic
AGTTACTGTGTCAACAAACT+TGG 0.447924 1:+89299683 None:intergenic
GACTGGGAATATTATAGAAA+AGG 0.455154 1:-89301616 MsG0180005262.01.T01:CDS
GAATATTATAGAAAAGGAAT+TGG 0.458956 1:-89301610 MsG0180005262.01.T01:CDS
TAAGCCTAAATTTGATGCAC+TGG 0.467869 1:-89299652 MsG0180005262.01.T01:intron
AGGAGCCTGAGCCCATAGAC+TGG 0.471648 1:-89301633 MsG0180005262.01.T01:CDS
CTATAATATTCCCAGTCTAT+GGG 0.473165 1:+89301622 None:intergenic
ATTTGTTCTGATTCTGGCAA+TGG 0.477285 1:-89299018 MsG0180005262.01.T01:three_prime_UTR
AAATTCACGACGAGCTTCAT+CGG 0.482178 1:+89302430 None:intergenic
ACAGATGTTGCATTCAAAGC+CGG 0.489390 1:-89302494 MsG0180005262.01.T01:CDS
GCAAGGAAAACATCAACTTC+AGG 0.493258 1:+89299254 None:intergenic
TCAAATTTAGGCTTATATTG+AGG 0.499518 1:+89299660 None:intergenic
AGGAATTGGCACTCGTTTGG+TGG 0.500877 1:-89301596 MsG0180005262.01.T01:CDS
CTTCTTCAGCTCAGGATGCT+CGG 0.501457 1:+89299340 None:intergenic
CAGATGTTGCATTCAAAGCC+GGG 0.515624 1:-89302493 MsG0180005262.01.T01:CDS
TATTCCCAGTCTATGGGCTC+AGG 0.528775 1:+89301628 None:intergenic
AAGCCGGGAAGAAGATCGAT+TGG 0.530458 1:-89302478 MsG0180005262.01.T01:CDS
GGAAGAAGATCGATTGGGAC+GGG 0.534761 1:-89302472 MsG0180005262.01.T01:CDS
AAAGTACTCATCTGCTGTCA+TGG 0.536378 1:+89299364 None:intergenic
GTTACTGTGTCAACAAACTT+GGG 0.562398 1:+89299684 None:intergenic
AGCCGGGAAGAAGATCGATT+GGG 0.567684 1:-89302477 MsG0180005262.01.T01:CDS
GGAGCCTGAGCCCATAGACT+GGG 0.568751 1:-89301632 MsG0180005262.01.T01:CDS
GAAGATCGATTGGGACGGGA+TGG 0.577380 1:-89302468 MsG0180005262.01.T01:CDS
GGTTGAGCTTAAGGAAGCAG+AGG 0.581516 1:-89299539 MsG0180005262.01.T01:CDS
CTCTGGTAACGATAGTGTCA+AGG 0.588551 1:-89302619 None:intergenic
TATTATTTATTAAACCAGCA+AGG 0.594786 1:+89299237 None:intergenic
ACGATAGTGTCAAGGTGCGA+AGG 0.595692 1:-89302611 None:intergenic
ACTCGAAACAAAGTTCAGCC+AGG 0.598938 1:-89302369 MsG0180005262.01.T01:intron
GGAGATTAGGAACGATAACT+GGG 0.620776 1:-89299306 MsG0180005262.01.T01:CDS
AAAGCGAAAGCGAAAATGAG+CGG 0.621372 1:-89302533 MsG0180005262.01.T01:exon
GGGAAGAAGATCGATTGGGA+CGG 0.627371 1:-89302473 MsG0180005262.01.T01:CDS
GCTGAAGAAGCAATTTGATG+AGG 0.630857 1:-89299327 MsG0180005262.01.T01:CDS
TTACTGTGTCAACAAACTTG+GGG 0.648687 1:+89299685 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAAAATTATTTTTTTTAT+TGG - Chr1:89300693-89300712 MsG0180005262.01.T01:intron 0.0%
!! AAAAAAAATACTAATATAAG+GGG + Chr1:89300061-89300080 None:intergenic 10.0%
!! TAAAAATGAGAAATTAATAT+AGG - Chr1:89300583-89300602 MsG0180005262.01.T01:intron 10.0%
!!! ATATATTCTAATGTAAATTT+TGG + Chr1:89301413-89301432 None:intergenic 10.0%
!!! TTTTTAGTGTTTTTATTATT+AGG + Chr1:89302592-89302611 None:intergenic 10.0%
!!! TTTTTTTAATCTGTATTTAA+TGG - Chr1:89300843-89300862 MsG0180005262.01.T01:intron 10.0%
!! AAATAACAATATATTGAATG+AGG + Chr1:89301550-89301569 None:intergenic 15.0%
!! AATACATAATCTATTTGTAT+AGG + Chr1:89299664-89299683 None:intergenic 15.0%
!!! AATTATTTCTCAAATTGAAA+AGG - Chr1:89301469-89301488 MsG0180005262.01.T01:intron 15.0%
!!! TAATTTGATAACTACATAAT+TGG + Chr1:89299844-89299863 None:intergenic 15.0%
!!! TACATTATTTTTTTGTAACT+TGG - Chr1:89302368-89302387 MsG0180005262.01.T01:intron 15.0%
!!! TACTGTAAATATTTGTATTT+TGG + Chr1:89300743-89300762 None:intergenic 15.0%
!!! TAGTATTTTTTTTTTGTCAA+TGG - Chr1:89300068-89300087 MsG0180005262.01.T01:intron 15.0%
!! AATAACTTGACAACAAATAT+TGG + Chr1:89300672-89300691 None:intergenic 20.0%
!! AATGGTATGTAAAATTTGAA+TGG + Chr1:89301833-89301852 None:intergenic 20.0%
!! AGTTATTGCCTAAATATTTA+TGG - Chr1:89301748-89301767 MsG0180005262.01.T01:intron 20.0%
!! GAATATTATAGAAAAGGAAT+TGG - Chr1:89299970-89299989 MsG0180005262.01.T01:intron 20.0%
!! TAACATCAAAGTTGAATTAA+TGG + Chr1:89301851-89301870 None:intergenic 20.0%
!! TATTATTTATTAAACCAGCA+AGG + Chr1:89302346-89302365 None:intergenic 20.0%
!! TCATAATTATATAACTGAAC+TGG + Chr1:89301159-89301178 None:intergenic 20.0%
!! TGTAGTTATCAAATTATTAC+CGG - Chr1:89299848-89299867 MsG0180005262.01.T01:intron 20.0%
!!! AAAAACATTTTCCTGAAATT+GGG + Chr1:89302499-89302518 None:intergenic 20.0%
!!! GTTAAATTTTAACATGCATT+AGG + Chr1:89301389-89301408 None:intergenic 20.0%
!!! GTTTTATAGTTGTTTTTTTC+AGG - Chr1:89301246-89301265 MsG0180005262.01.T01:intron 20.0%
!!! TTCTCAGTTTTATGTAATAT+TGG - Chr1:89302146-89302165 MsG0180005262.01.T01:intron 20.0%
! AAGTACATAAGAAAAGAAAC+TGG - Chr1:89301492-89301511 MsG0180005262.01.T01:intron 25.0%
! AATAAATTGTTGTGTTTGTC+TGG - Chr1:89302530-89302549 MsG0180005262.01.T01:exon 25.0%
! AATGTCTATCAATTTCAGAT+AGG + Chr1:89301320-89301339 None:intergenic 25.0%
! AATTATGAACCATAGTTTAC+TGG - Chr1:89301171-89301190 MsG0180005262.01.T01:intron 25.0%
! AGGATATTGATAAATTCAAC+TGG - Chr1:89300193-89300212 MsG0180005262.01.T01:intron 25.0%
! ATGTCTATCAATTTCAGATA+GGG + Chr1:89301319-89301338 None:intergenic 25.0%
! ATTTAGAAACTGTTGTCTTA+TGG - Chr1:89302459-89302478 MsG0180005262.01.T01:CDS 25.0%
! CATTAGAATATATGAAACTG+TGG - Chr1:89301420-89301439 MsG0180005262.01.T01:intron 25.0%
! GACTACATCCATAAATATTT+AGG + Chr1:89301759-89301778 None:intergenic 25.0%
! GTATTCAAAACATGATGAAA+AGG + Chr1:89299634-89299653 None:intergenic 25.0%
! TAAAACCTGGAAATTGTATA+TGG - Chr1:89301588-89301607 MsG0180005262.01.T01:CDS 25.0%
! TATCATTTCTTATTAATCGC+AGG - Chr1:89299927-89299946 MsG0180005262.01.T01:intron 25.0%
! TCAAATTTAGGCTTATATTG+AGG + Chr1:89301923-89301942 None:intergenic 25.0%
!! AAAAAGATAATCTTGATAGC+TGG + Chr1:89301011-89301030 None:intergenic 25.0%
!! AGCTATCAAGATTATCTTTT+TGG - Chr1:89301010-89301029 MsG0180005262.01.T01:intron 25.0%
!! CAAAAACATTTTCCTGAAAT+TGG + Chr1:89302500-89302519 None:intergenic 25.0%
!! CTTCATCATCAATTTTGAAT+TGG - Chr1:89299397-89299416 MsG0180005262.01.T01:intron 25.0%
!! TTTTCTGTGAAAACAAACAA+TGG + Chr1:89302187-89302206 None:intergenic 25.0%
!!! AGTTCAGTTTTTTTAAGGAT+CGG + Chr1:89300355-89300374 None:intergenic 25.0%
!!! ATCATGTTTTAGTGCATTTT+CGG - Chr1:89299429-89299448 MsG0180005262.01.T01:intron 25.0%
!!! ATTGTTTGTTTTCACAGAAA+AGG - Chr1:89302186-89302205 MsG0180005262.01.T01:intron 25.0%
!!! ATTTCAGGAAAATGTTTTTG+TGG - Chr1:89302500-89302519 MsG0180005262.01.T01:CDS 25.0%
!!! CAAACAGTTCAGTTTTTTTA+AGG + Chr1:89300360-89300379 None:intergenic 25.0%
AAGAATCTGAACGTTTGAAA+AGG - Chr1:89302075-89302094 MsG0180005262.01.T01:intron 30.0%
AATTATATAACTGAACTGGC+TGG + Chr1:89301155-89301174 None:intergenic 30.0%
AGATTATGTATTCAGTTACG+AGG - Chr1:89299672-89299691 MsG0180005262.01.T01:CDS 30.0%
AGCATCTAAGAACAAAATGA+AGG + Chr1:89299302-89299321 None:intergenic 30.0%
ATGCACGATTAATAAATGCA+AGG + Chr1:89301658-89301677 None:intergenic 30.0%
ATTGAATGAGGCAAAGTTAT+AGG + Chr1:89301538-89301557 None:intergenic 30.0%
ATTTCCCAACATCATTACAA+AGG + Chr1:89299538-89299557 None:intergenic 30.0%
CGAATGAGACAAAAAGATAT+TGG + Chr1:89301050-89301069 None:intergenic 30.0%
CTATAATATTCCCAGTCTAT+GGG + Chr1:89299961-89299980 None:intergenic 30.0%
CTGATACTTAATAGCTTGAA+CGG + Chr1:89301217-89301236 None:intergenic 30.0%
CTTGATTGATAGAGAAAAGT+TGG + Chr1:89301979-89301998 None:intergenic 30.0%
GACTGGGAATATTATAGAAA+AGG - Chr1:89299964-89299983 MsG0180005262.01.T01:intron 30.0%
GGTATGTAAAATTTGAATGG+CGG + Chr1:89301830-89301849 None:intergenic 30.0%
GTACCATTGAATACAAGAAT+CGG + Chr1:89300221-89300240 None:intergenic 30.0%
GTATACATTTGTTCTGATTC+TGG - Chr1:89302556-89302575 MsG0180005262.01.T01:five_prime_UTR 30.0%
GTTATTTGACCTATTCAGAT+GGG - Chr1:89301563-89301582 MsG0180005262.01.T01:intron 30.0%
TAAGCAAAACTGCAACTAAT+GGG + Chr1:89301131-89301150 None:intergenic 30.0%
TCTATAATATTCCCAGTCTA+TGG + Chr1:89299962-89299981 None:intergenic 30.0%
TCTTCCCTATGAAAAGAATA+TGG + Chr1:89300243-89300262 None:intergenic 30.0%
TGATACCATATACAATTTCC+AGG + Chr1:89301596-89301615 None:intergenic 30.0%
TGTACCTTTGTAATGATGTT+GGG - Chr1:89299531-89299550 MsG0180005262.01.T01:CDS 30.0%
TGTCTATCAATTTCAGATAG+GGG + Chr1:89301318-89301337 None:intergenic 30.0%
TTGTACCTTTGTAATGATGT+TGG - Chr1:89299530-89299549 MsG0180005262.01.T01:CDS 30.0%
TTTGTAATGATGTTGGGAAA+TGG - Chr1:89299537-89299556 MsG0180005262.01.T01:CDS 30.0%
! AAAGCAGTTTTACAAACAGT+TGG - Chr1:89300624-89300643 MsG0180005262.01.T01:intron 30.0%
! ATATATGAAACTGTGGATTG+TGG - Chr1:89301427-89301446 MsG0180005262.01.T01:intron 30.0%
! CTGCAGTTGTCAAAATTTTA+TGG - Chr1:89302423-89302442 MsG0180005262.01.T01:CDS 30.0%
! GGTACCATATTCTTTTCATA+GGG - Chr1:89300236-89300255 MsG0180005262.01.T01:intron 30.0%
! TCGTGCATTGAATTGATTTA+AGG - Chr1:89301670-89301689 MsG0180005262.01.T01:intron 30.0%
! TGGTACCATATTCTTTTCAT+AGG - Chr1:89300235-89300254 MsG0180005262.01.T01:intron 30.0%
! TGGTTTTTCTCCAGTTTAAA+AGG - Chr1:89302388-89302407 MsG0180005262.01.T01:CDS 30.0%
! TGTTATTTGACCTATTCAGA+TGG - Chr1:89301562-89301581 MsG0180005262.01.T01:intron 30.0%
!! ACTGAACTACTTTTGAAGAA+TGG + Chr1:89300433-89300452 None:intergenic 30.0%
!!! GTTCAGTTTTTTTAAGGATC+GGG + Chr1:89300354-89300373 None:intergenic 30.0%
!!! TTCAGTTTTTTTAAGGATCG+GGG + Chr1:89300353-89300372 None:intergenic 30.0%
!!! TTGTTTTTTTCAGGTCAGTT+AGG - Chr1:89301255-89301274 MsG0180005262.01.T01:intron 30.0%
AGCAATTTGATGAGGAGATT+AGG - Chr1:89302261-89302280 MsG0180005262.01.T01:intron 35.0%
AGGTAATAGAAATCACCACA+TGG - Chr1:89302122-89302141 MsG0180005262.01.T01:intron 35.0%
AGTTACTGTGTCAACAAACT+TGG + Chr1:89301900-89301919 None:intergenic 35.0%
AGTTATAGGAAACGTTGTCA+TGG + Chr1:89301524-89301543 None:intergenic 35.0%
ATACCAACACTTCTGTTTGA+AGG - Chr1:89299759-89299778 MsG0180005262.01.T01:intron 35.0%
ATGAGATGTCAATTGTGCTA+AGG + Chr1:89300559-89300578 None:intergenic 35.0%
ATGCACTTTCTCTTCTTTAG+GGG - Chr1:89300158-89300177 MsG0180005262.01.T01:intron 35.0%
ATTCAGATGGGAATAAAACC+TGG - Chr1:89301575-89301594 MsG0180005262.01.T01:CDS 35.0%
ATTTGTTCTGATTCTGGCAA+TGG - Chr1:89302562-89302581 MsG0180005262.01.T01:five_prime_UTR 35.0%
CATAAAACTGAGAAACCATG+TGG + Chr1:89302140-89302159 None:intergenic 35.0%
CATGCACTTTCTCTTCTTTA+GGG - Chr1:89300157-89300176 MsG0180005262.01.T01:intron 35.0%
CCAAAAAAAACTGAACTGTC+CGG - Chr1:89300315-89300334 MsG0180005262.01.T01:intron 35.0%
CTAAGCAAAACTGCAACTAA+TGG + Chr1:89301132-89301151 None:intergenic 35.0%
CTTACCAGTGCATCAAATTT+AGG + Chr1:89301935-89301954 None:intergenic 35.0%
GAAATTAGAGAGATCTGTGA+AGG + Chr1:89299247-89299266 None:intergenic 35.0%
GATCCGATTCTTGTATTCAA+TGG - Chr1:89300215-89300234 MsG0180005262.01.T01:intron 35.0%
GTTACTGTGTCAACAAACTT+GGG + Chr1:89301899-89301918 None:intergenic 35.0%
GTTATAGGAAACGTTGTCAT+GGG + Chr1:89301523-89301542 None:intergenic 35.0%
TAAGCCTAAATTTGATGCAC+TGG - Chr1:89301928-89301947 MsG0180005262.01.T01:intron 35.0%
TAATTCACAAAAGCCATCCT+CGG - Chr1:89300271-89300290 MsG0180005262.01.T01:intron 35.0%
TACTTTGGTCCAGTAAACTA+TGG + Chr1:89301183-89301202 None:intergenic 35.0%
TCATGCACTTTCTCTTCTTT+AGG - Chr1:89300156-89300175 MsG0180005262.01.T01:intron 35.0%
TGAGATGTCAATTGTGCTAA+GGG + Chr1:89300558-89300577 None:intergenic 35.0%
TGATGTTATGCTTCTTCTGT+AGG - Chr1:89301863-89301882 MsG0180005262.01.T01:intron 35.0%
TGTGAAGGAATTTATCTACC+TGG + Chr1:89299232-89299251 None:intergenic 35.0%
TTACTGTGTCAACAAACTTG+GGG + Chr1:89301898-89301917 None:intergenic 35.0%
! ATGTGGAGAACCTTTTAAAC+TGG + Chr1:89302401-89302420 None:intergenic 35.0%
! ATGTGTTTTCTTGCTGAAGT+TGG - Chr1:89302020-89302039 MsG0180005262.01.T01:intron 35.0%
! GTAAAATTTGAATGGCGGAA+TGG + Chr1:89301825-89301844 None:intergenic 35.0%
! TTTTGACAACTGCAGAAATG+TGG + Chr1:89302418-89302437 None:intergenic 35.0%
!! TCAGGAAAATGTTTTTGTGG+CGG - Chr1:89302503-89302522 MsG0180005262.01.T01:CDS 35.0%
!! TGCTAGATCGATTGACTTTT+AGG - Chr1:89299318-89299337 MsG0180005262.01.T01:CDS 35.0%
!! TTAGAGCATGACTTGTTTGA+TGG - Chr1:89300107-89300126 MsG0180005262.01.T01:intron 35.0%
!!! GGTTTTATTCCCATCTGAAT+AGG + Chr1:89301575-89301594 None:intergenic 35.0%
AAAAGCCATCCTCGGAAATT+AGG - Chr1:89300279-89300298 MsG0180005262.01.T01:intron 40.0%
AAAAGGAATTGGCACTCGTT+TGG - Chr1:89299981-89300000 MsG0180005262.01.T01:intron 40.0%
AAAGCCATCCTCGGAAATTA+GGG - Chr1:89300280-89300299 MsG0180005262.01.T01:intron 40.0%
AAAGCGAAAGCGAAAATGAG+CGG - Chr1:89299047-89299066 MsG0180005262.01.T01:three_prime_UTR 40.0%
AAATTCACGACGAGCTTCAT+CGG + Chr1:89299153-89299172 None:intergenic 40.0%
ACAGATGTTGCATTCAAAGC+CGG - Chr1:89299086-89299105 MsG0180005262.01.T01:three_prime_UTR 40.0%
ACTGGACCAAAGTACAAGTT+CGG - Chr1:89301189-89301208 MsG0180005262.01.T01:intron 40.0%
AGACATTCTTTCGTTGTGTC+TGG - Chr1:89301333-89301352 MsG0180005262.01.T01:intron 40.0%
AGGAGATTAGGAACGATAAC+TGG - Chr1:89302273-89302292 MsG0180005262.01.T01:intron 40.0%
ATGAACAACCCTAATTTCCG+AGG + Chr1:89300291-89300310 None:intergenic 40.0%
CATACATAGTGAGAGAGATG+AGG - Chr1:89301787-89301806 MsG0180005262.01.T01:intron 40.0%
CATTCGCAAAACGTGATGAT+GGG - Chr1:89301064-89301083 MsG0180005262.01.T01:intron 40.0%
GCAAGGAAAACATCAACTTC+AGG + Chr1:89302329-89302348 None:intergenic 40.0%
GCTGAAGAAGCAATTTGATG+AGG - Chr1:89302253-89302272 MsG0180005262.01.T01:intron 40.0%
GGAGATTAGGAACGATAACT+GGG - Chr1:89302274-89302293 MsG0180005262.01.T01:intron 40.0%
TATTCAGTTACGAGGCTTAG+AGG - Chr1:89299680-89299699 MsG0180005262.01.T01:CDS 40.0%
TCAAATTGCTTCTTCAGCTC+AGG + Chr1:89302251-89302270 None:intergenic 40.0%
TCAGACACACTAACAAGTGT+CGG + Chr1:89299797-89299816 None:intergenic 40.0%
TCATTCGCAAAACGTGATGA+TGG - Chr1:89301063-89301082 MsG0180005262.01.T01:intron 40.0%
TTTAGGGGTAAAGTGGTAGA+AGG - Chr1:89300173-89300192 MsG0180005262.01.T01:intron 40.0%
! AAAGTACTCATCTGCTGTCA+TGG + Chr1:89302219-89302238 None:intergenic 40.0%
! AAGCCTTCAAACAGAAGTGT+TGG + Chr1:89299765-89299784 None:intergenic 40.0%
! ATTCGCAAAACGTGATGATG+GGG - Chr1:89301065-89301084 MsG0180005262.01.T01:intron 40.0%
! TCTCTTCTTTAGGGGTAAAG+TGG - Chr1:89300166-89300185 MsG0180005262.01.T01:intron 40.0%
!! AATTTCAGATAGGGGTGTTC+GGG + Chr1:89301310-89301329 None:intergenic 40.0%
!! CAATTTCAGATAGGGGTGTT+CGG + Chr1:89301311-89301330 None:intergenic 40.0%
!!! CCGGACAGTTCAGTTTTTTT+TGG + Chr1:89300318-89300337 None:intergenic 40.0%
!!! GAAGTTGATGTTTTCCTTGC+TGG - Chr1:89302329-89302348 MsG0180005262.01.T01:intron 40.0%
ACAACCCTAATTTCCGAGGA+TGG + Chr1:89300287-89300306 None:intergenic 45.0%
ACGGATCCGAACTTGTACTT+TGG + Chr1:89301198-89301217 None:intergenic 45.0%
ACTCGAAACAAAGTTCAGCC+AGG - Chr1:89299211-89299230 MsG0180005262.01.T01:exon 45.0%
AGAGAGAGCTGACAACAGTT+TGG + Chr1:89300469-89300488 None:intergenic 45.0%
CAGATGTTGCATTCAAAGCC+GGG - Chr1:89299087-89299106 MsG0180005262.01.T01:three_prime_UTR 45.0%
CAGTCAAGACACCCAATTTC+AGG - Chr1:89302485-89302504 MsG0180005262.01.T01:CDS 45.0%
TGCTAATGTACAGTCGCTGA+AGG - Chr1:89302102-89302121 MsG0180005262.01.T01:intron 45.0%
! GCTGAAGTTGGTTGAGCTTA+AGG - Chr1:89302032-89302051 MsG0180005262.01.T01:intron 45.0%
! TTTTGTGCGAACTGAACTGC+TGG - Chr1:89300382-89300401 MsG0180005262.01.T01:intron 45.0%
!! ATTTCAGATAGGGGTGTTCG+GGG + Chr1:89301309-89301328 None:intergenic 45.0%
!! CACTAACAAGTGTCGGACAT+CGG + Chr1:89299790-89299809 None:intergenic 45.0%
!! TTTTAAGGATCGGGGCAGTT+GGG + Chr1:89300345-89300364 None:intergenic 45.0%
!! TTTTTAAGGATCGGGGCAGT+TGG + Chr1:89300346-89300365 None:intergenic 45.0%
!! AAAAAAAAAATACTAATATA+AGG + Chr1:89300063-89300082 None:intergenic 5.0%
!! AAAAAAAAATACTAATATAA+GGG + Chr1:89300062-89300081 None:intergenic 5.0%
AAGCCGGGAAGAAGATCGAT+TGG - Chr1:89299102-89299121 MsG0180005262.01.T01:three_prime_UTR 50.0%
AGCCGGGAAGAAGATCGATT+GGG - Chr1:89299103-89299122 MsG0180005262.01.T01:three_prime_UTR 50.0%
AGGAATTGGCACTCGTTTGG+TGG - Chr1:89299984-89300003 MsG0180005262.01.T01:intron 50.0%
CACCTGACACATCACAACAC+CGG + Chr1:89299870-89299889 None:intergenic 50.0%
CTTCTTCAGCTCAGGATGCT+CGG + Chr1:89302243-89302262 None:intergenic 50.0%
GGAAGAAGATCGATTGGGAC+GGG - Chr1:89299108-89299127 MsG0180005262.01.T01:three_prime_UTR 50.0%
GGGAAGAAGATCGATTGGGA+CGG - Chr1:89299107-89299126 MsG0180005262.01.T01:three_prime_UTR 50.0%
GGTTGAGCTTAAGGAAGCAG+AGG - Chr1:89302041-89302060 MsG0180005262.01.T01:intron 50.0%
GTCCCAATCGATCTTCTTCC+CGG + Chr1:89299108-89299127 None:intergenic 50.0%
TATTCCCAGTCTATGGGCTC+AGG + Chr1:89299955-89299974 None:intergenic 50.0%
!! AAACGTGATGATGGGGTGGA+AGG - Chr1:89301072-89301091 MsG0180005262.01.T01:intron 50.0%
!! TACCGGTGTTGTGATGTGTC+AGG - Chr1:89299865-89299884 MsG0180005262.01.T01:intron 50.0%
GAAGATCGATTGGGACGGGA+TGG - Chr1:89299112-89299131 MsG0180005262.01.T01:three_prime_UTR 55.0%
!! AGATAGGGGTGTTCGGGGTT+GGG + Chr1:89301304-89301323 None:intergenic 55.0%
!! CGCAAAACGTGATGATGGGG+TGG - Chr1:89301068-89301087 MsG0180005262.01.T01:intron 55.0%
AGGAGCCTGAGCCCATAGAC+TGG - Chr1:89299947-89299966 MsG0180005262.01.T01:intron 60.0%
ATCGGGGCAGTTGGGTTCTC+CGG + Chr1:89300337-89300356 None:intergenic 60.0%
GGAGCCTGAGCCCATAGACT+GGG - Chr1:89299948-89299967 MsG0180005262.01.T01:intron 60.0%
!! CAGATAGGGGTGTTCGGGGT+TGG + Chr1:89301305-89301324 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 89298974 89302628 89298974 ID=MsG0180005262.01;Name=MsG0180005262.01
Chr1 mRNA 89298974 89302628 89298974 ID=MsG0180005262.01.T01;Parent=MsG0180005262.01;Name=MsG0180005262.01.T01;_AED=0.41;_eAED=0.41;_QI=88|0.75|0.8|1|1|1|5|243|182
Chr1 exon 89298974 89299399 89298974 ID=MsG0180005262.01.T01:exon:17230;Parent=MsG0180005262.01.T01
Chr1 exon 89299516 89299563 89299516 ID=MsG0180005262.01.T01:exon:17229;Parent=MsG0180005262.01.T01
Chr1 exon 89299653 89299717 89299653 ID=MsG0180005262.01.T01:exon:17228;Parent=MsG0180005262.01.T01
Chr1 exon 89301572 89301653 89301572 ID=MsG0180005262.01.T01:exon:17227;Parent=MsG0180005262.01.T01
Chr1 exon 89302370 89302628 89302370 ID=MsG0180005262.01.T01:exon:17226;Parent=MsG0180005262.01.T01
Chr1 five_prime_UTR 89302541 89302628 89302541 ID=MsG0180005262.01.T01:five_prime_utr;Parent=MsG0180005262.01.T01
Chr1 CDS 89302370 89302540 89302370 ID=MsG0180005262.01.T01:cds;Parent=MsG0180005262.01.T01
Chr1 CDS 89301572 89301653 89301572 ID=MsG0180005262.01.T01:cds;Parent=MsG0180005262.01.T01
Chr1 CDS 89299653 89299717 89299653 ID=MsG0180005262.01.T01:cds;Parent=MsG0180005262.01.T01
Chr1 CDS 89299516 89299563 89299516 ID=MsG0180005262.01.T01:cds;Parent=MsG0180005262.01.T01
Chr1 CDS 89299217 89299399 89299217 ID=MsG0180005262.01.T01:cds;Parent=MsG0180005262.01.T01
Chr1 three_prime_UTR 89298974 89299216 89298974 ID=MsG0180005262.01.T01:three_prime_utr;Parent=MsG0180005262.01.T01
Gene Sequence

>MsG0180005262.01.T01

ATGAGCGGAACAGTGAAAAAAGTTACAGATGTTGCATTCAAAGCCGGGAAGAAGATCGATTGGGACGGGATGGCTAAGCTTCTCGTCACCGATGAAGCTCGTCGTGAATTTTTCAATCTTCGTCGCGCTTTCGACGAAGTTAACACTCAACTCGAAACAAAGTTCAGCCAGGAGCCTGAGCCCATAGACTGGGAATATTATAGAAAAGGAATTGGCACTCGTTTGGTGGATATGTACAAGCAGCATTATGAGAGCATTGAGATCCCCAAGTTTGTTGACACAGTAACTCCTCAATATAAGCCTAAATTTGATGCACTGTTGGTTGAGCTTAAGGAAGCAGAGGAGAAATCTCTGAAGAATCTGAACAAAAGGATTTCTACCATGACAGCAGATGAGTACTTTGCCGAGCATCCTGAGCTGAAGAAGCAATTTGATGAGGAGATTAGGAACGATAACTGGGCTATTCACACTACAAAACTGAGCAAAACGCCTGAAGTTGATGTTTTCCTTGCTGGTTTAATAAATAATAACTACATTATTTTTTTGTAA
Protein sequence

>MsG0180005262.01.T01

MSGTVKKVTDVAFKAGKKIDWDGMAKLLVTDEARREFFNLRRAFDEVNTQLETKFSQEPEPIDWEYYRKGIGTRLVDMYKQHYESIEIPKFVDTVTPQYKPKFDALLVELKEAEEKSLKNLNKRISTMTADEYFAEHPELKKQFDEEIRNDNWAIHTTKLSKTPEVDVFLAGLINNNYIIFL*