AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023769.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023769.01.T01 MTR_8g101880 98.347 121 2 0 1 121 1 121 2.44e-87 249
MsG0480023769.01.T01 MTR_8g101890 78.333 120 26 0 2 121 3 122 1.91e-70 207
MsG0480023769.01.T01 MTR_2g100380 61.667 120 46 0 2 121 98 217 9.26e-52 162
MsG0480023769.01.T01 MTR_5g062980 69.492 59 18 0 58 116 1 59 2.41e-26 95.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023769.01.T01 AT4G26210 73.950 119 31 0 2 120 3 121 5.45e-66 196
MsG0480023769.01.T01 AT4G26210 73.950 119 31 0 2 120 3 121 5.45e-66 196
MsG0480023769.01.T01 AT4G29480 73.950 119 31 0 2 120 3 121 9.53e-66 195
MsG0480023769.01.T01 AT2G19680 73.950 119 31 0 2 120 3 121 2.56e-65 194
MsG0480023769.01.T01 AT2G19680 73.950 119 31 0 2 120 3 121 2.56e-65 194

Find 40 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATAACATTATGTTGATTT+TGG 0.100120 4:+89597379 MsG0480023769.01.T01:three_prime_UTR
TTCCATGCTTAGTTATAAAT+TGG 0.149886 4:-89595424 None:intergenic
TTGATTATGTCAAGCAAATA+TGG 0.268577 4:+89597162 MsG0480023769.01.T01:CDS
TGAAAGGACTCACAACGATT+TGG 0.345795 4:-89597131 None:intergenic
AAAACCATGCATAGCATTCC+AGG 0.362552 4:-89597238 None:intergenic
ATGACAGTACTCCTGGCTTT+AGG 0.369756 4:-89597356 None:intergenic
TCCATGCTTAGTTATAAATT+GGG 0.411328 4:-89595423 None:intergenic
GAAGAACAAGGAATACATTC+AGG 0.438061 4:+89595472 MsG0480023769.01.T01:CDS
GTTATAAATGACAGTACTCC+TGG 0.444865 4:-89597363 None:intergenic
GGAAGGGGATTTACTTTCAC+AGG 0.448226 4:+89597278 MsG0480023769.01.T01:CDS
TGGCATTTCTCTACTGTAGC+AGG 0.457610 4:-89595497 None:intergenic
TTCTTACCACAAGCAGCTGT+TGG 0.459464 4:+89595448 MsG0480023769.01.T01:CDS
TTTATAATGTTCGATTTAGC+GGG 0.460232 4:+89597324 MsG0480023769.01.T01:three_prime_UTR
GAATGAATAGACGCAGTAAT+CGG 0.467972 4:-89594703 None:intergenic
TCGTTGTGAGTCCTTTCATA+AGG 0.471099 4:+89597136 MsG0480023769.01.T01:CDS
CTTTATAATGTTCGATTTAG+CGG 0.477701 4:+89597323 MsG0480023769.01.T01:three_prime_UTR
AACACACATCACACAATCCT+TGG 0.494909 4:+89594640 MsG0480023769.01.T01:five_prime_UTR
TGCTGGTCAGATTGTTGGAA+GGG 0.499361 4:+89597262 MsG0480023769.01.T01:CDS
TAACCTCTTGTATTTGAAGC+GGG 0.510997 4:+89597477 MsG0480023769.01.T01:three_prime_UTR
AGTAGAGAAATGCCAACTTC+TGG 0.523939 4:+89595505 MsG0480023769.01.T01:CDS
ATAGTTGCTTGGCCAGAAGT+TGG 0.524773 4:-89595517 None:intergenic
TCCCAATTTATAACTAAGCA+TGG 0.525968 4:+89595422 MsG0480023769.01.T01:CDS
CTAAATCGAACATTATAAAG+TGG 0.546080 4:-89597320 None:intergenic
ATAATCAAGTTCCTTATGAA+AGG 0.546893 4:-89597147 None:intergenic
GGCTCTATACTCCTAAAGCC+AGG 0.548608 4:+89597345 MsG0480023769.01.T01:three_prime_UTR
GCTGGTCAGATTGTTGGAAG+GGG 0.563270 4:+89597263 MsG0480023769.01.T01:CDS
GGTTAAAATAAAACCTAGCA+AGG 0.573767 4:-89595550 None:intergenic
GTGCTGGTCAGATTGTTGGA+AGG 0.577540 4:+89597261 MsG0480023769.01.T01:CDS
GTAACCTCTTGTATTTGAAG+CGG 0.579495 4:+89597476 MsG0480023769.01.T01:three_prime_UTR
GCGAGTGTAAAATAGTTGCT+TGG 0.582756 4:-89595528 None:intergenic
TCTTCTCCAACAGCTGCTTG+TGG 0.584533 4:-89595454 None:intergenic
GCAGCGTCGCGAGGTAGCAG+CGG 0.590854 4:+89594670 MsG0480023769.01.T01:five_prime_UTR
TTGGCCTGGAATGCTATGCA+TGG 0.593962 4:+89597234 MsG0480023769.01.T01:CDS
TTATCATGTTAGAAAGCTAG+CGG 0.599612 4:+89597520 MsG0480023769.01.T01:three_prime_UTR
GATTTGAAGATTGAAGATGC+AGG 0.608817 4:+89597197 MsG0480023769.01.T01:CDS
GCAGCTGTTGGAGAAGAACA+AGG 0.648148 4:+89595460 MsG0480023769.01.T01:CDS
ACTGCGTCTATTCATTCACA+AGG 0.658506 4:+89594709 MsG0480023769.01.T01:five_prime_UTR
TATCCCGCTTCAAATACAAG+AGG 0.658735 4:-89597480 None:intergenic
TCAAATACAAGAGGTTACTG+AGG 0.675220 4:-89597471 None:intergenic
GCGACGCTGCAAAAGCACCA+AGG 0.703860 4:-89594657 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATGATTAAAAAAAATTAC+AGG - Chr4:89597557-89597576 None:intergenic 10.0%
!! AGTTTAAAAAAAAAAAATTG+AGG - Chr4:89596090-89596109 None:intergenic 10.0%
!! GAAAATTTATTGAATATTTA+GGG + Chr4:89594919-89594938 MsG0480023769.01.T01:intron 10.0%
!! TCTTTAATTATTAAATATGA+TGG + Chr4:89596260-89596279 MsG0480023769.01.T01:intron 10.0%
!! TGAAAATTTATTGAATATTT+AGG + Chr4:89594918-89594937 MsG0480023769.01.T01:intron 10.0%
!!! GATTTTATAAAATTAACTTA+TGG + Chr4:89595137-89595156 MsG0480023769.01.T01:intron 10.0%
!! TAGATTTATTAATAAGGAAA+GGG - Chr4:89595574-89595593 None:intergenic 15.0%
!!! GTTTTATTAAAGAGTTAAAA+GGG + Chr4:89596110-89596129 MsG0480023769.01.T01:intron 15.0%
!!! TATAACATTATGTTGATTTT+GGG + Chr4:89597380-89597399 MsG0480023769.01.T01:three_prime_UTR 15.0%
!!! TGTTTTATTAAAGAGTTAAA+AGG + Chr4:89596109-89596128 MsG0480023769.01.T01:intron 15.0%
!!! TTATAACATTATGTTGATTT+TGG + Chr4:89597379-89597398 MsG0480023769.01.T01:three_prime_UTR 15.0%
!!! TTTAGGTATTATTTTTTTGT+CGG + Chr4:89595000-89595019 MsG0480023769.01.T01:intron 15.0%
!! AAAAATCACTTACAATCAAT+AGG - Chr4:89595641-89595660 None:intergenic 20.0%
!! AATAGAAACAAACTAAAAGT+AGG - Chr4:89596195-89596214 None:intergenic 20.0%
!! CTAGATTTATTAATAAGGAA+AGG - Chr4:89595575-89595594 None:intergenic 20.0%
!! TAGAAAAATACACGAAATTA+TGG - Chr4:89596289-89596308 None:intergenic 20.0%
!! TATACAGTATAAAAGATGAT+CGG + Chr4:89596447-89596466 MsG0480023769.01.T01:intron 20.0%
!! TTCATCTATGAAATAAAGAA+CGG - Chr4:89594752-89594771 None:intergenic 20.0%
!!! AACTTTGAAGAGATAAAAAT+TGG - Chr4:89594883-89594902 None:intergenic 20.0%
!!! TGTTTAGATTAGCTAAATTT+AGG + Chr4:89594983-89595002 MsG0480023769.01.T01:intron 20.0%
!!! TTTTTTTTGCATTTTGATGT+CGG + Chr4:89595666-89595685 MsG0480023769.01.T01:intron 20.0%
! AAAGTAACATCAATGCATAA+TGG + Chr4:89595212-89595231 MsG0480023769.01.T01:intron 25.0%
! ATAATCAAGTTCCTTATGAA+AGG - Chr4:89597150-89597169 None:intergenic 25.0%
! ATACAGTATAAAAGATGATC+GGG + Chr4:89596448-89596467 MsG0480023769.01.T01:intron 25.0%
! ATAGCTAAAAGAAGAAACTA+CGG - Chr4:89597026-89597045 None:intergenic 25.0%
! CAAAAGACAAATGTACAATA+AGG - Chr4:89595357-89595376 None:intergenic 25.0%
! CTAAATCGAACATTATAAAG+TGG - Chr4:89597323-89597342 None:intergenic 25.0%
! CTTTATAATGTTCGATTTAG+CGG + Chr4:89597323-89597342 MsG0480023769.01.T01:three_prime_UTR 25.0%
! GATTGAACAATCAACATATT+AGG - Chr4:89595771-89595790 None:intergenic 25.0%
! TCCAGCTAGATTTATTAATA+AGG - Chr4:89595580-89595599 None:intergenic 25.0%
! TCCATGCTTAGTTATAAATT+GGG - Chr4:89595426-89595445 None:intergenic 25.0%
! TCCTTATTAATAAATCTAGC+TGG + Chr4:89595576-89595595 MsG0480023769.01.T01:intron 25.0%
! TGAACAATTATAATTCGTGT+TGG + Chr4:89595061-89595080 MsG0480023769.01.T01:intron 25.0%
! TTCCATGCTTAGTTATAAAT+TGG - Chr4:89595427-89595446 None:intergenic 25.0%
! TTGAAGAGATAAAAATTGGT+CGG - Chr4:89594879-89594898 None:intergenic 25.0%
! TTGATTATGTCAAGCAAATA+TGG + Chr4:89597162-89597181 MsG0480023769.01.T01:CDS 25.0%
! TTGTACTAAATAGCTTATTG+TGG - Chr4:89596605-89596624 None:intergenic 25.0%
! TTTATAATGTTCGATTTAGC+GGG + Chr4:89597324-89597343 MsG0480023769.01.T01:three_prime_UTR 25.0%
!! ATATACCACTATAGCATTTT+AGG + Chr4:89596374-89596393 MsG0480023769.01.T01:intron 25.0%
!! TATACCACTATAGCATTTTA+GGG + Chr4:89596375-89596394 MsG0480023769.01.T01:intron 25.0%
!!! GTTTTGTTGTTTTGTACTTT+AGG + Chr4:89596236-89596255 MsG0480023769.01.T01:intron 25.0%
AAAAAAGGTTGAGAAACTAG+TGG + Chr4:89597440-89597459 MsG0480023769.01.T01:three_prime_UTR 30.0%
ACATGCTAGAAACCTAAATT+TGG - Chr4:89594966-89594985 None:intergenic 30.0%
ACCATTAACTTTGTAGTTCA+GGG - Chr4:89595812-89595831 None:intergenic 30.0%
ATGAAATAAAGAACGGAAGA+AGG - Chr4:89594745-89594764 None:intergenic 30.0%
CAAATGTACAATAAGGTACA+AGG - Chr4:89595350-89595369 None:intergenic 30.0%
CATGCTAGAAACCTAAATTT+GGG - Chr4:89594965-89594984 None:intergenic 30.0%
CTATAACTACGTATATCTTG+AGG + Chr4:89595239-89595258 MsG0480023769.01.T01:intron 30.0%
GGTTAAAATAAAACCTAGCA+AGG - Chr4:89595553-89595572 None:intergenic 30.0%
GTGAACTCTAAACTAACTAA+GGG - Chr4:89596538-89596557 None:intergenic 30.0%
TAAGACTTCATTGCTAAGAA+AGG - Chr4:89596496-89596515 None:intergenic 30.0%
TACAAAATACTGTGCAATAG+TGG + Chr4:89596736-89596755 MsG0480023769.01.T01:intron 30.0%
TATAGTGGTATATCGAATAG+CGG - Chr4:89596367-89596386 None:intergenic 30.0%
TCCCAATTTATAACTAAGCA+TGG + Chr4:89595422-89595441 MsG0480023769.01.T01:CDS 30.0%
TCGCTAATATATGTACTTGT+TGG + Chr4:89595615-89595634 MsG0480023769.01.T01:intron 30.0%
TGTGAACTCTAAACTAACTA+AGG - Chr4:89596539-89596558 None:intergenic 30.0%
TGTTGAATTGAAACATCAGA+AGG + Chr4:89596921-89596940 MsG0480023769.01.T01:intron 30.0%
TGTTGAATTGAAACATCAGA+TGG + Chr4:89596921-89596940 MsG0480023769.01.T01:intron 30.0%
TTATCATGTTAGAAAGCTAG+CGG + Chr4:89597520-89597539 MsG0480023769.01.T01:three_prime_UTR 30.0%
TTCAAGAGTGAAACTCAAAT+TGG - Chr4:89595045-89595064 None:intergenic 30.0%
! ATACCACTATAGCATTTTAG+GGG + Chr4:89596376-89596395 MsG0480023769.01.T01:intron 30.0%
! ATTTGTCATTCTTTTCTGTG+AGG + Chr4:89597416-89597435 MsG0480023769.01.T01:three_prime_UTR 30.0%
! ATTTTCTCCCTTGTCAATAA+AGG - Chr4:89595720-89595739 None:intergenic 30.0%
! TCTTTTCTGTGAGGAAAAAA+AGG + Chr4:89597425-89597444 MsG0480023769.01.T01:three_prime_UTR 30.0%
! TGCATTTAGAACCCAAATTT+AGG + Chr4:89594951-89594970 MsG0480023769.01.T01:intron 30.0%
! TTTAAAGTGACTTGTCAGTT+AGG + Chr4:89595279-89595298 MsG0480023769.01.T01:intron 30.0%
! TTTTCTCCCTTGTCAATAAA+GGG - Chr4:89595719-89595738 None:intergenic 30.0%
!!! CTATTGTTACTCTCGTTTTT+TGG + Chr4:89594796-89594815 MsG0480023769.01.T01:intron 30.0%
!!! TCTCTTCAAAGTTTTGTTGT+TGG + Chr4:89594890-89594909 MsG0480023769.01.T01:intron 30.0%
AAATAAAGAACGGAAGAAGG+AGG - Chr4:89594742-89594761 None:intergenic 35.0%
AATATGACACTGTTGCATAG+TGG + Chr4:89596769-89596788 MsG0480023769.01.T01:intron 35.0%
AATCCCCTAAAATGCTATAG+TGG - Chr4:89596382-89596401 None:intergenic 35.0%
ACACTGTGAACTCTAAACTA+AGG - Chr4:89596543-89596562 None:intergenic 35.0%
ACGGAAGGAATAATATGGAT+GGG + Chr4:89595302-89595321 MsG0480023769.01.T01:intron 35.0%
AGCCAATTACAGTGAATTTG+CGG + Chr4:89596018-89596037 MsG0480023769.01.T01:intron 35.0%
ATAGTGGTATATCGAATAGC+GGG - Chr4:89596366-89596385 None:intergenic 35.0%
ATCACATGAACCCTAATTCA+TGG - Chr4:89596873-89596892 None:intergenic 35.0%
CACTGTGAACTCTAAACTAA+GGG - Chr4:89596542-89596561 None:intergenic 35.0%
CCATTAACTTTGTAGTTCAG+GGG - Chr4:89595811-89595830 None:intergenic 35.0%
GAAGAACAAGGAATACATTC+AGG + Chr4:89595472-89595491 MsG0480023769.01.T01:CDS 35.0%
GAATGAATAGACGCAGTAAT+CGG - Chr4:89594706-89594725 None:intergenic 35.0%
GACCATTAACTTTGTAGTTC+AGG - Chr4:89595813-89595832 None:intergenic 35.0%
GAGAGAAATCAGATCTTAAG+AGG - Chr4:89594825-89594844 None:intergenic 35.0%
GATTTGAAGATTGAAGATGC+AGG + Chr4:89597197-89597216 MsG0480023769.01.T01:CDS 35.0%
GTAACCTCTTGTATTTGAAG+CGG + Chr4:89597476-89597495 MsG0480023769.01.T01:three_prime_UTR 35.0%
GTTATAAATGACAGTACTCC+TGG - Chr4:89597366-89597385 None:intergenic 35.0%
TATTGATCAGAATTCCCATG+TGG + Chr4:89596982-89597001 MsG0480023769.01.T01:intron 35.0%
TCAAATACAAGAGGTTACTG+AGG - Chr4:89597474-89597493 None:intergenic 35.0%
TGTTGGATATCATAGAGTTC+AGG + Chr4:89596847-89596866 MsG0480023769.01.T01:intron 35.0%
TTAGGACGGAAGGAATAATA+TGG + Chr4:89595297-89595316 MsG0480023769.01.T01:intron 35.0%
! TAACCTCTTGTATTTGAAGC+GGG + Chr4:89597477-89597496 MsG0480023769.01.T01:three_prime_UTR 35.0%
!! ACTATAGCATTTTAGGGGAT+TGG + Chr4:89596381-89596400 MsG0480023769.01.T01:intron 35.0%
!!! GGTATTATTTTTTTGTCGGC+TGG + Chr4:89595004-89595023 MsG0480023769.01.T01:intron 35.0%
AAAACCATGCATAGCATTCC+AGG - Chr4:89597241-89597260 None:intergenic 40.0%
AACACACATCACACAATCCT+TGG + Chr4:89594640-89594659 MsG0480023769.01.T01:five_prime_UTR 40.0%
ACTGCGTCTATTCATTCACA+AGG + Chr4:89594709-89594728 MsG0480023769.01.T01:five_prime_UTR 40.0%
AGAGTTCAGGCCATGAATTA+GGG + Chr4:89596860-89596879 MsG0480023769.01.T01:intron 40.0%
AGTAGAGAAATGCCAACTTC+TGG + Chr4:89595505-89595524 MsG0480023769.01.T01:CDS 40.0%
ATTGACAAGGGAGAAAATCC+TGG + Chr4:89595722-89595741 MsG0480023769.01.T01:intron 40.0%
CCCCTGAACTACAAAGTTAA+TGG + Chr4:89595808-89595827 MsG0480023769.01.T01:intron 40.0%
CGGAAGGAATAATATGGATG+GGG + Chr4:89595303-89595322 MsG0480023769.01.T01:intron 40.0%
GACGGAAGGAATAATATGGA+TGG + Chr4:89595301-89595320 MsG0480023769.01.T01:intron 40.0%
GCGAGTGTAAAATAGTTGCT+TGG - Chr4:89595531-89595550 None:intergenic 40.0%
TAGAGTTCAGGCCATGAATT+AGG + Chr4:89596859-89596878 MsG0480023769.01.T01:intron 40.0%
TATCCCGCTTCAAATACAAG+AGG - Chr4:89597483-89597502 None:intergenic 40.0%
TCGTTGTGAGTCCTTTCATA+AGG + Chr4:89597136-89597155 MsG0480023769.01.T01:CDS 40.0%
TGAAAGGACTCACAACGATT+TGG - Chr4:89597134-89597153 None:intergenic 40.0%
TGATATCCAACACTGAACAC+AGG - Chr4:89596839-89596858 None:intergenic 40.0%
TTCCCTCCCTTTATTGACAA+GGG + Chr4:89595710-89595729 MsG0480023769.01.T01:intron 40.0%
TTGTCAATAAAGGGAGGGAA+GGG - Chr4:89595710-89595729 None:intergenic 40.0%
TTGTCAATAGCGTGCTATAG+CGG + Chr4:89595909-89595928 MsG0480023769.01.T01:intron 40.0%
! AAGTGACTTGTCAGTTAGGA+CGG + Chr4:89595283-89595302 MsG0480023769.01.T01:intron 40.0%
! TATTGCTGACTGTGGTTTGT+GGG - Chr4:89595984-89596003 None:intergenic 40.0%
! TATTTTACACTCGCCTTGCT+AGG + Chr4:89595537-89595556 MsG0480023769.01.T01:CDS 40.0%
!! TTTTGTGCTGGTCAGATTGT+TGG + Chr4:89597257-89597276 MsG0480023769.01.T01:CDS 40.0%
!!! GCAGGTATTGCTGTTTTGTT+TGG + Chr4:89597215-89597234 MsG0480023769.01.T01:CDS 40.0%
!!! TATTGCTGTTTTGTTTGGCC+TGG + Chr4:89597220-89597239 MsG0480023769.01.T01:CDS 40.0%
AACACAGGTGTAGTCAACTG+TGG - Chr4:89596824-89596843 None:intergenic 45.0%
AACGTATCCGTGGTAGGATT+TGG + Chr4:89595935-89595954 MsG0480023769.01.T01:intron 45.0%
ACAAGGGAGAAAATCCTGGA+GGG + Chr4:89595726-89595745 MsG0480023769.01.T01:intron 45.0%
ACTACACCTGTGTTCAGTGT+TGG + Chr4:89596830-89596849 MsG0480023769.01.T01:intron 45.0%
AGCAGACACGATAGCAGTTT+GGG - Chr4:89596045-89596064 None:intergenic 45.0%
ATAGTTGCTTGGCCAGAAGT+TGG - Chr4:89595520-89595539 None:intergenic 45.0%
ATCCGTGGTAGGATTTGGAA+CGG + Chr4:89595940-89595959 MsG0480023769.01.T01:intron 45.0%
CAAACCACAGTCAGCAATAG+TGG + Chr4:89595985-89596004 MsG0480023769.01.T01:intron 45.0%
CTCCCTTGTCAATAAAGGGA+GGG - Chr4:89595715-89595734 None:intergenic 45.0%
CTGTGCAATAGTGGCTATAG+CGG + Chr4:89596745-89596764 MsG0480023769.01.T01:intron 45.0%
CTTCCCTCCCTTTATTGACA+AGG + Chr4:89595709-89595728 MsG0480023769.01.T01:intron 45.0%
CTTGTCAATAAAGGGAGGGA+AGG - Chr4:89595711-89595730 None:intergenic 45.0%
GGAAGGGGATTTACTTTCAC+AGG + Chr4:89597278-89597297 MsG0480023769.01.T01:CDS 45.0%
GGCCGCAAATTCACTGTAAT+TGG - Chr4:89596023-89596042 None:intergenic 45.0%
TAGCAGACACGATAGCAGTT+TGG - Chr4:89596046-89596065 None:intergenic 45.0%
TCTCCCTTGTCAATAAAGGG+AGG - Chr4:89595716-89595735 None:intergenic 45.0%
TGCTGGTCAGATTGTTGGAA+GGG + Chr4:89597262-89597281 MsG0480023769.01.T01:CDS 45.0%
TGGCATTTCTCTACTGTAGC+AGG - Chr4:89595500-89595519 None:intergenic 45.0%
TTCTTACCACAAGCAGCTGT+TGG + Chr4:89595448-89595467 MsG0480023769.01.T01:CDS 45.0%
! ATAGCGGAATAACGTATCCG+TGG + Chr4:89595925-89595944 MsG0480023769.01.T01:intron 45.0%
! ATGACAGTACTCCTGGCTTT+AGG - Chr4:89597359-89597378 None:intergenic 45.0%
! CTATTGCTGACTGTGGTTTG+TGG - Chr4:89595985-89596004 None:intergenic 45.0%
!! GCCTCTAAAAAGCACCACAT+GGG - Chr4:89596999-89597018 None:intergenic 45.0%
!! TCCCATGTGGTGCTTTTTAG+AGG + Chr4:89596995-89597014 MsG0480023769.01.T01:intron 45.0%
!! TGCCTCTAAAAAGCACCACA+TGG - Chr4:89597000-89597019 None:intergenic 45.0%
!!! TGCTATGCATGGTTTTGTGC+TGG + Chr4:89597245-89597264 MsG0480023769.01.T01:CDS 45.0%
!!! AAATGTAATTAATTTTAAAA+AGG + Chr4:89595104-89595123 MsG0480023769.01.T01:intron 5.0%
AACTTGAAACCTCACCCTCC+AGG - Chr4:89595743-89595762 None:intergenic 50.0%
AAGATGATCGGGTGATCGTG+TGG + Chr4:89596459-89596478 MsG0480023769.01.T01:intron 50.0%
ACCACAGTCAGCAATAGTGG+TGG + Chr4:89595988-89596007 MsG0480023769.01.T01:intron 50.0%
CGGAATAACGTATCCGTGGT+AGG + Chr4:89595929-89595948 MsG0480023769.01.T01:intron 50.0%
CTGCTATCGTGTCTGCTAGT+AGG + Chr4:89596048-89596067 MsG0480023769.01.T01:intron 50.0%
GACAAGGGAGAAAATCCTGG+AGG + Chr4:89595725-89595744 MsG0480023769.01.T01:intron 50.0%
GACTTGTCAGTTAGGACGGA+AGG + Chr4:89595287-89595306 MsG0480023769.01.T01:intron 50.0%
GCAGACACGATAGCAGTTTG+GGG - Chr4:89596044-89596063 None:intergenic 50.0%
GCAGCTGTTGGAGAAGAACA+AGG + Chr4:89595460-89595479 MsG0480023769.01.T01:CDS 50.0%
GCTGGTCAGATTGTTGGAAG+GGG + Chr4:89597263-89597282 MsG0480023769.01.T01:CDS 50.0%
GGCTCTATACTCCTAAAGCC+AGG + Chr4:89597345-89597364 MsG0480023769.01.T01:three_prime_UTR 50.0%
GTCCGTTCCAAATCCTACCA+CGG - Chr4:89595945-89595964 None:intergenic 50.0%
TCTTCTCCAACAGCTGCTTG+TGG - Chr4:89595457-89595476 None:intergenic 50.0%
TTGGCCTGGAATGCTATGCA+TGG + Chr4:89597234-89597253 MsG0480023769.01.T01:CDS 50.0%
! ACCACCACTATTGCTGACTG+TGG - Chr4:89595992-89596011 None:intergenic 50.0%
! GGGATTGGTGCATGCTATCT+GGG + Chr4:89596396-89596415 MsG0480023769.01.T01:intron 50.0%
! GTGCTGGTCAGATTGTTGGA+AGG + Chr4:89597261-89597280 MsG0480023769.01.T01:CDS 50.0%
GGAGAAAATCCTGGAGGGTG+AGG + Chr4:89595731-89595750 MsG0480023769.01.T01:intron 55.0%
! AGGATTTGGAACGGACCGCA+CGG + Chr4:89595949-89595968 MsG0480023769.01.T01:intron 55.0%
! GGGGATTGGTGCATGCTATC+TGG + Chr4:89596395-89596414 MsG0480023769.01.T01:intron 55.0%
! AACGGACCGCACGGTGCTTT+TGG + Chr4:89595958-89595977 MsG0480023769.01.T01:intron 60.0%
!! GCGACGCTGCAAAAGCACCA+AGG - Chr4:89594660-89594679 None:intergenic 60.0%
!! TGTGGGCCAAAAGCACCGTG+CGG - Chr4:89595967-89595986 None:intergenic 60.0%
! GGTGCTTTTGCAGCGTCGCG+AGG + Chr4:89594661-89594680 MsG0480023769.01.T01:five_prime_UTR 65.0%
GCAGCGTCGCGAGGTAGCAG+CGG + Chr4:89594670-89594689 MsG0480023769.01.T01:five_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 89594569 89597643 89594569 ID=MsG0480023769.01;Name=MsG0480023769.01
Chr4 mRNA 89594569 89597643 89594569 ID=MsG0480023769.01.T01;Parent=MsG0480023769.01;Name=MsG0480023769.01.T01;_AED=0.44;_eAED=0.44;_QI=162|1|1|1|0.5|0.33|3|330|121
Chr4 exon 89594569 89594730 89594569 ID=MsG0480023769.01.T01:exon:25297;Parent=MsG0480023769.01.T01
Chr4 exon 89595380 89595558 89595380 ID=MsG0480023769.01.T01:exon:25298;Parent=MsG0480023769.01.T01
Chr4 exon 89597127 89597643 89597127 ID=MsG0480023769.01.T01:exon:25299;Parent=MsG0480023769.01.T01
Chr4 five_prime_UTR 89594569 89594730 89594569 ID=MsG0480023769.01.T01:five_prime_utr;Parent=MsG0480023769.01.T01
Chr4 CDS 89595380 89595558 89595380 ID=MsG0480023769.01.T01:cds;Parent=MsG0480023769.01.T01
Chr4 CDS 89597127 89597313 89597127 ID=MsG0480023769.01.T01:cds;Parent=MsG0480023769.01.T01
Chr4 three_prime_UTR 89597314 89597643 89597314 ID=MsG0480023769.01.T01:three_prime_utr;Parent=MsG0480023769.01.T01
Gene Sequence

>MsG0480023769.01.T01

ATGTCGAAGTTGCAGCAGTTGACATCAAAAGCGTGTCAAGCGTCCCAATTTATAACTAAGCATGGAACTTCTTACCACAAGCAGCTGTTGGAGAAGAACAAGGAATACATTCAGGATCCTGCTACAGTAGAGAAATGCCAACTTCTGGCCAAGCAACTATTTTACACTCGCCTTGCTAGTATTCCAAATCGTTGTGAGTCCTTTCATAAGGAACTTGATTATGTCAAGCAAATATGGAAGAATAAGCAAGATTTGAAGATTGAAGATGCAGGTATTGCTGTTTTGTTTGGCCTGGAATGCTATGCATGGTTTTGTGCTGGTCAGATTGTTGGAAGGGGATTTACTTTCACAGGATACTATGTCTGA
Protein sequence

>MsG0480023769.01.T01

MSKLQQLTSKACQASQFITKHGTSYHKQLLEKNKEYIQDPATVEKCQLLAKQLFYTRLASIPNRCESFHKELDYVKQIWKNKQDLKIEDAGIAVLFGLECYAWFCAGQIVGRGFTFTGYYV*