AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680035577.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035577.01.T01 MTR_6g084520 100.000 97 0 0 1 97 1 97 6.16e-70 204
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035577.01.T01 AT3G18410 78.351 97 21 0 1 97 1 97 4.05e-55 167
MsG0680035577.01.T01 AT3G18410 78.351 97 21 0 1 97 1 97 4.05e-55 167
MsG0680035577.01.T01 AT1G49140 76.289 97 23 0 1 97 1 97 1.02e-53 163
MsG0680035577.01.T01 AT1G49140 76.289 97 23 0 1 97 1 97 1.02e-53 163

Find 28 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAAGCTTCGTTGGTGTTAT+AGG 0.264593 6:+108667719 MsG0680035577.01.T01:CDS
GGTCGTCACCCTTCTCTTCA+TGG 0.286257 6:+108667829 MsG0680035577.01.T01:CDS
AGTCTACTAGAGGGATTGGT+TGG 0.361455 6:+108667797 MsG0680035577.01.T01:CDS
ATCGCCACTGGATCTTTGTA+TGG 0.363135 6:-108667628 None:intergenic
TTGTTGATTAAGGGTATGTT+TGG 0.369681 6:+108668066 MsG0680035577.01.T01:intron
CAAAGATCCAGTGGCGATGC+TGG 0.412298 6:+108667633 MsG0680035577.01.T01:CDS
CGTTGGTGTTATAGGATTGA+AGG 0.432641 6:+108667727 MsG0680035577.01.T01:CDS
GTCTACTAGAGGGATTGGTT+GGG 0.443343 6:+108667798 MsG0680035577.01.T01:CDS
CTCATTTCCAGCATCGCCAC+TGG 0.446593 6:-108667640 None:intergenic
CTTGAGTCTACTAGAGGGAT+TGG 0.477507 6:+108667793 MsG0680035577.01.T01:CDS
GGGAAGGAAGAAGGGGTATG+TGG 0.484400 6:+108667564 MsG0680035577.01.T01:CDS
TCTACTAGAGGGATTGGTTG+GGG 0.490687 6:+108667799 MsG0680035577.01.T01:CDS
CAGTGGCGATGCTGGAAATG+AGG 0.508085 6:+108667641 MsG0680035577.01.T01:CDS
TTCGCCGGATCGAAATCATC+TGG 0.510493 6:-108667604 None:intergenic
GGATCTTTGTATGGGTTCGC+CGG 0.524941 6:-108667619 None:intergenic
TCGCCACTGGATCTTTGTAT+GGG 0.545031 6:-108667627 None:intergenic
GTTGGTGTTATAGGATTGAA+GGG 0.554470 6:+108667728 MsG0680035577.01.T01:CDS
AAACACAAACCATGAAGAGA+AGG 0.554780 6:-108667838 None:intergenic
TTATTCGTGAAAAGCTTCGT+TGG 0.561335 6:+108667710 MsG0680035577.01.T01:CDS
GCCGGATCGAAATCATCTGG+TGG 0.568519 6:-108667601 None:intergenic
AGTGGCGATGCTGGAAATGA+GGG 0.574162 6:+108667642 MsG0680035577.01.T01:CDS
CCCACCAGATGATTTCGATC+CGG 0.583849 6:+108667600 MsG0680035577.01.T01:CDS
CAGTATCTTGAGTCTACTAG+AGG 0.595830 6:+108667787 MsG0680035577.01.T01:CDS
CTACTAGAGGGATTGGTTGG+GGG 0.604522 6:+108667800 MsG0680035577.01.T01:CDS
CCGGATCGAAATCATCTGGT+GGG 0.615720 6:-108667600 None:intergenic
AACACAAACCATGAAGAGAA+GGG 0.648401 6:-108667837 None:intergenic
AGTATCTTGAGTCTACTAGA+GGG 0.663002 6:+108667788 MsG0680035577.01.T01:CDS
GAACCCATACAAAGATCCAG+TGG 0.708004 6:+108667624 MsG0680035577.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCTCTTTTTGATATATAAT+TGG + Chr6:108667873-108667892 MsG0680035577.01.T01:intron 15.0%
!! TTGTTGTGAAATTATGAAAT+GGG + Chr6:108667899-108667918 MsG0680035577.01.T01:intron 20.0%
!!! GATTTTTATATAGCATTGAA+GGG + Chr6:108667947-108667966 MsG0680035577.01.T01:intron 20.0%
!!! TGATTTTTATATAGCATTGA+AGG + Chr6:108667946-108667965 MsG0680035577.01.T01:intron 20.0%
!!! TTGATGTTTTTGTTGATTAA+GGG + Chr6:108668057-108668076 MsG0680035577.01.T01:intron 20.0%
!!! TTTGATGTTTTTGTTGATTA+AGG + Chr6:108668056-108668075 MsG0680035577.01.T01:intron 20.0%
!!! TTTGTTGTGAAATTATGAAA+TGG + Chr6:108667898-108667917 MsG0680035577.01.T01:intron 20.0%
!!! TTGTATAATGGGTTTTTGTT+GGG + Chr6:108667971-108667990 MsG0680035577.01.T01:intron 25.0%
!!! TTTGTATAATGGGTTTTTGT+TGG + Chr6:108667970-108667989 MsG0680035577.01.T01:intron 25.0%
AACACATTGTTCGTGAAAAA+TGG + Chr6:108667665-108667684 MsG0680035577.01.T01:CDS 30.0%
CATTGAAGGGTTTGTATAAT+GGG + Chr6:108667960-108667979 MsG0680035577.01.T01:intron 30.0%
TTTGTTGGGAATAGTGAAAT+GGG + Chr6:108667985-108668004 MsG0680035577.01.T01:intron 30.0%
! TGATTTTTATGTCGTATGGA+AGG + Chr6:108668032-108668051 MsG0680035577.01.T01:intron 30.0%
! TTGTTGATTAAGGGTATGTT+TGG + Chr6:108668066-108668085 MsG0680035577.01.T01:intron 30.0%
! TTTTGTTGGGAATAGTGAAA+TGG + Chr6:108667984-108668003 MsG0680035577.01.T01:intron 30.0%
AAACACAAACCATGAAGAGA+AGG - Chr6:108667841-108667860 None:intergenic 35.0%
AACACAAACCATGAAGAGAA+GGG - Chr6:108667840-108667859 None:intergenic 35.0%
AGTATCTTGAGTCTACTAGA+GGG + Chr6:108667788-108667807 MsG0680035577.01.T01:CDS 35.0%
ATCTCCGAAAGAGTGAAAAA+TGG + Chr6:108667543-108667562 MsG0680035577.01.T01:exon 35.0%
GCATTGAAGGGTTTGTATAA+TGG + Chr6:108667959-108667978 MsG0680035577.01.T01:intron 35.0%
TCTCCGAAAGAGTGAAAAAT+GGG + Chr6:108667544-108667563 MsG0680035577.01.T01:exon 35.0%
TGAAAAATGGGAAGGAAGAA+GGG + Chr6:108667556-108667575 MsG0680035577.01.T01:exon 35.0%
TTATTCGTGAAAAGCTTCGT+TGG + Chr6:108667710-108667729 MsG0680035577.01.T01:CDS 35.0%
! GCTGTGATTTTTATGTCGTA+TGG + Chr6:108668028-108668047 MsG0680035577.01.T01:intron 35.0%
!! AAAAGCTTCGTTGGTGTTAT+AGG + Chr6:108667719-108667738 MsG0680035577.01.T01:CDS 35.0%
!! CTTCCCATTTTTCACTCTTT+CGG - Chr6:108667550-108667569 None:intergenic 35.0%
!! GTTGGTGTTATAGGATTGAA+GGG + Chr6:108667728-108667747 MsG0680035577.01.T01:CDS 35.0%
CAGTATCTTGAGTCTACTAG+AGG + Chr6:108667787-108667806 MsG0680035577.01.T01:CDS 40.0%
CGAAAGAGTGAAAAATGGGA+AGG + Chr6:108667548-108667567 MsG0680035577.01.T01:exon 40.0%
GAAAAATGGGAAGGAAGAAG+GGG + Chr6:108667557-108667576 MsG0680035577.01.T01:exon 40.0%
GTGAAAAATGGGAAGGAAGA+AGG + Chr6:108667555-108667574 MsG0680035577.01.T01:exon 40.0%
!! CGTTGGTGTTATAGGATTGA+AGG + Chr6:108667727-108667746 MsG0680035577.01.T01:CDS 40.0%
ATCGCCACTGGATCTTTGTA+TGG - Chr6:108667631-108667650 None:intergenic 45.0%
CTTGAGTCTACTAGAGGGAT+TGG + Chr6:108667793-108667812 MsG0680035577.01.T01:CDS 45.0%
GAACCCATACAAAGATCCAG+TGG + Chr6:108667624-108667643 MsG0680035577.01.T01:CDS 45.0%
TCGCCACTGGATCTTTGTAT+GGG - Chr6:108667630-108667649 None:intergenic 45.0%
! AGTCTACTAGAGGGATTGGT+TGG + Chr6:108667797-108667816 MsG0680035577.01.T01:CDS 45.0%
!! GTCTACTAGAGGGATTGGTT+GGG + Chr6:108667798-108667817 MsG0680035577.01.T01:CDS 45.0%
!! TCTACTAGAGGGATTGGTTG+GGG + Chr6:108667799-108667818 MsG0680035577.01.T01:CDS 45.0%
AGTGGCGATGCTGGAAATGA+GGG + Chr6:108667642-108667661 MsG0680035577.01.T01:CDS 50.0%
CCCACCAGATGATTTCGATC+CGG + Chr6:108667600-108667619 MsG0680035577.01.T01:CDS 50.0%
CCGGATCGAAATCATCTGGT+GGG - Chr6:108667603-108667622 None:intergenic 50.0%
GGATCTTTGTATGGGTTCGC+CGG - Chr6:108667622-108667641 None:intergenic 50.0%
TTCGCCGGATCGAAATCATC+TGG - Chr6:108667607-108667626 None:intergenic 50.0%
!! CTACTAGAGGGATTGGTTGG+GGG + Chr6:108667800-108667819 MsG0680035577.01.T01:CDS 50.0%
CAAAGATCCAGTGGCGATGC+TGG + Chr6:108667633-108667652 MsG0680035577.01.T01:CDS 55.0%
CAGTGGCGATGCTGGAAATG+AGG + Chr6:108667641-108667660 MsG0680035577.01.T01:CDS 55.0%
CTCATTTCCAGCATCGCCAC+TGG - Chr6:108667643-108667662 None:intergenic 55.0%
GCCGGATCGAAATCATCTGG+TGG - Chr6:108667604-108667623 None:intergenic 55.0%
GGTCGTCACCCTTCTCTTCA+TGG + Chr6:108667829-108667848 MsG0680035577.01.T01:CDS 55.0%
! GGGAAGGAAGAAGGGGTATG+TGG + Chr6:108667564-108667583 MsG0680035577.01.T01:CDS 55.0%
!! GGGATTGGTTGGGGGAAAGA+TGG + Chr6:108667808-108667827 MsG0680035577.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr6 gene 108667530 108668112 108667530 ID=MsG0680035577.01;Name=MsG0680035577.01
Chr6 mRNA 108667530 108668112 108667530 ID=MsG0680035577.01.T01;Parent=MsG0680035577.01;Name=MsG0680035577.01.T01;_AED=0.47;_eAED=0.52;_QI=32|0|0.5|1|1|1|2|0|107
Chr6 exon 108667530 108667850 108667530 ID=MsG0680035577.01.T01:exon:8221;Parent=MsG0680035577.01.T01
Chr6 exon 108668078 108668112 108668078 ID=MsG0680035577.01.T01:exon:8222;Parent=MsG0680035577.01.T01
Chr6 five_prime_UTR 108667530 108667561 108667530 ID=MsG0680035577.01.T01:five_prime_utr;Parent=MsG0680035577.01.T01
Chr6 CDS 108667562 108667850 108667562 ID=MsG0680035577.01.T01:cds;Parent=MsG0680035577.01.T01
Chr6 CDS 108668078 108668112 108668078 ID=MsG0680035577.01.T01:cds;Parent=MsG0680035577.01.T01
Gene Sequence

>MsG0680035577.01.T01

ATGGGAAGGAAGAAGGGGTATGTGGAGTTCGATGACAACCCACCAGATGATTTCGATCCGGCGAACCCATACAAAGATCCAGTGGCGATGCTGGAAATGAGGGAACACATTGTTCGTGAAAAATGGATTCAGATCGAAAAAGCAAAGATTATTCGTGAAAAGCTTCGTTGGTGTTATAGGATTGAAGGGATTAATCATCATCAGAAATGTCGTCATCTTGTGAAACAGTATCTTGAGTCTACTAGAGGGATTGGTTGGGGGAAAGATGGTCGTCACCCTTCTCTTCATGGGTATGTTTGGATTGATTTAGTTGAGCTTATATGA

Protein sequence

>MsG0680035577.01.T01

MGRKKGYVEFDDNPPDDFDPANPYKDPVAMLEMREHIVREKWIQIEKAKIIREKLRWCYRIEGINHHQKCRHLVKQYLESTRGIGWGKDGRHPSLHGYVWIDLVELI*