AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005371.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005371.01.T01 MTR_1g098310 100.000 86 0 0 1 86 1 86 5.42e-57 170
MsG0180005371.01.T01 MTR_3g104640 60.465 43 17 0 43 85 67 109 1.85e-12 58.2
MsG0180005371.01.T01 MTR_4g125990 54.386 57 23 1 29 85 69 122 4.60e-12 57.4
MsG0180005371.01.T01 MTR_2g099630 50.000 48 24 0 38 85 59 106 7.88e-11 53.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005371.01.T01 AT4G09890 55.914 93 27 4 1 86 2 87 1.28e-24 88.6
MsG0180005371.01.T01 AT5G11970 54.545 44 20 0 42 85 60 103 6.35e-12 56.6
MsG0180005371.01.T01 AT3G62640 37.209 86 44 2 10 85 25 110 6.10e-11 54.3
MsG0180005371.01.T01 AT1G72720 47.170 53 28 0 34 86 74 126 8.03e-11 54.3

Find 16 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTGTTCTTACACTGAATT+TGG 0.137121 1:-90925119 MsG0180005371.01.T01:CDS
TTCCACTGCCAACACATTAT+AGG 0.253589 1:+90924989 None:intergenic
TTCAATGGACCAAGTGATAA+AGG 0.349768 1:-90925068 MsG0180005371.01.T01:CDS
GTGTAAGAACAAGAGTATTC+AGG 0.369295 1:+90925128 None:intergenic
TCCATTGAAATTATATGAGT+TGG 0.413854 1:+90925082 None:intergenic
CAAATGATTCAGAGCTTAAG+AGG 0.471110 1:-90925028 MsG0180005371.01.T01:CDS
TCCAACTCATATAATTTCAA+TGG 0.471387 1:-90925083 MsG0180005371.01.T01:CDS
AAACCACTTCCTTTATCACT+TGG 0.503804 1:+90925059 None:intergenic
AGAACAAGTTCAGCGATGTT+CGG 0.522118 1:-90924923 MsG0180005371.01.T01:CDS
GGACCAAGTGATAAAGGAAG+TGG 0.529951 1:-90925062 MsG0180005371.01.T01:CDS
GTCCTATAATGTGTTGGCAG+TGG 0.532233 1:-90924991 MsG0180005371.01.T01:CDS
TATAATGTGTTGGCAGTGGA+AGG 0.566724 1:-90924987 MsG0180005371.01.T01:CDS
AAGTTCAGCGATGTTCGGTA+TGG 0.569967 1:-90924918 MsG0180005371.01.T01:CDS
GTGTAAGAAACAGCTTCAAG+TGG 0.577880 1:-90924950 MsG0180005371.01.T01:CDS
AATCAAGTCCTATAATGTGT+TGG 0.586946 1:-90924997 MsG0180005371.01.T01:CDS
CTGAATTTGGATACAGTGAA+AGG 0.593305 1:-90925106 MsG0180005371.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TCCAACTCATATAATTTCAA+TGG - Chr1:90924977-90924996 MsG0180005371.01.T01:CDS 25.0%
! TCCATTGAAATTATATGAGT+TGG + Chr1:90924981-90925000 None:intergenic 25.0%
AATCAAGTCCTATAATGTGT+TGG - Chr1:90925063-90925082 MsG0180005371.01.T01:CDS 30.0%
TCTTGTTCTTACACTGAATT+TGG - Chr1:90924941-90924960 MsG0180005371.01.T01:CDS 30.0%
AAACCACTTCCTTTATCACT+TGG + Chr1:90925004-90925023 None:intergenic 35.0%
CAAATGATTCAGAGCTTAAG+AGG - Chr1:90925032-90925051 MsG0180005371.01.T01:CDS 35.0%
CTGAATTTGGATACAGTGAA+AGG - Chr1:90924954-90924973 MsG0180005371.01.T01:CDS 35.0%
TTCAATGGACCAAGTGATAA+AGG - Chr1:90924992-90925011 MsG0180005371.01.T01:CDS 35.0%
!! GTGTAAGAACAAGAGTATTC+AGG + Chr1:90924935-90924954 None:intergenic 35.0%
AGAACAAGTTCAGCGATGTT+CGG - Chr1:90925137-90925156 MsG0180005371.01.T01:CDS 40.0%
GTGTAAGAAACAGCTTCAAG+TGG - Chr1:90925110-90925129 MsG0180005371.01.T01:CDS 40.0%
TTCCACTGCCAACACATTAT+AGG + Chr1:90925074-90925093 None:intergenic 40.0%
! TATAATGTGTTGGCAGTGGA+AGG - Chr1:90925073-90925092 MsG0180005371.01.T01:CDS 40.0%
AAGTTCAGCGATGTTCGGTA+TGG - Chr1:90925142-90925161 MsG0180005371.01.T01:CDS 45.0%
GGACCAAGTGATAAAGGAAG+TGG - Chr1:90924998-90925017 MsG0180005371.01.T01:CDS 45.0%
! GTCCTATAATGTGTTGGCAG+TGG - Chr1:90925069-90925088 MsG0180005371.01.T01:CDS 45.0%
Chromosome Type Strat End Strand Name
Chr1 gene 90924911 90925171 90924911 ID=MsG0180005371.01;Name=MsG0180005371.01
Chr1 mRNA 90924911 90925171 90924911 ID=MsG0180005371.01.T01;Parent=MsG0180005371.01;Name=MsG0180005371.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|86
Chr1 exon 90924911 90925171 90924911 ID=MsG0180005371.01.T01:exon:38120;Parent=MsG0180005371.01.T01
Chr1 CDS 90924911 90925171 90924911 ID=MsG0180005371.01.T01:cds;Parent=MsG0180005371.01.T01
Gene Sequence

>MsG0180005371.01.T01

ATGGAGAAAAGCAAGTCATTTCCTGAATACTCTTGTTCTTACACTGAATTTGGATACAGTGAAAGGTCCAACTCATATAATTTCAATGGACCAAGTGATAAAGGAAGTGGTTTTTCTGCATCAAATGATTCAGAGCTTAAGAGGAAGAAGAGAATCAAGTCCTATAATGTGTTGGCAGTGGAAGGAAAGCTTAAAAGTAGTGTAAGAAACAGCTTCAAGTGGATCAAGAACAAGTTCAGCGATGTTCGGTATGGCGTGTGA

Protein sequence

>MsG0180005371.01.T01

MEKSKSFPEYSCSYTEFGYSERSNSYNFNGPSDKGSGFSASNDSELKRKKRIKSYNVLAVEGKLKSSVRNSFKWIKNKFSDVRYGV*