Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005371.01.T01 | XP_003592063.1 | 100 | 86 | 0 | 0 | 1 | 86 | 1 | 86 | 4.48E-53 | 170 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047869.01 | MsG0180005371.01 | 0.824009 | 1.017960e-53 | 8.906824e-51 |
| MsG0080048764.01 | MsG0180005371.01 | 0.822180 | 2.719482e-53 | 2.259339e-50 |
| MsG0080048856.01 | MsG0180005371.01 | 0.821649 | 3.609209e-53 | 2.953719e-50 |
| MsG0080048874.01 | MsG0180005371.01 | 0.828851 | 7.145261e-55 | 7.187142e-52 |
| MsG0180000065.01 | MsG0180005371.01 | 0.838122 | 3.468255e-57 | 4.600490e-54 |
| MsG0180000152.01 | MsG0180005371.01 | 0.805855 | 1.090982e-49 | 5.834419e-47 |
| MsG0180000178.01 | MsG0180005371.01 | 0.807103 | 5.951420e-50 | 3.285945e-47 |
| MsG0180000334.01 | MsG0180005371.01 | 0.809376 | 1.950626e-50 | 1.143432e-47 |
| MsG0180000335.01 | MsG0180005371.01 | 0.815183 | 1.053186e-51 | 7.210222e-49 |
| MsG0180000470.01 | MsG0180005371.01 | 0.822004 | 2.986493e-53 | 2.468876e-50 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005371.01.T01 | MTR_1g098310 | 100.000 | 86 | 0 | 0 | 1 | 86 | 1 | 86 | 5.42e-57 | 170 |
| MsG0180005371.01.T01 | MTR_3g104640 | 60.465 | 43 | 17 | 0 | 43 | 85 | 67 | 109 | 1.85e-12 | 58.2 |
| MsG0180005371.01.T01 | MTR_4g125990 | 54.386 | 57 | 23 | 1 | 29 | 85 | 69 | 122 | 4.60e-12 | 57.4 |
| MsG0180005371.01.T01 | MTR_2g099630 | 50.000 | 48 | 24 | 0 | 38 | 85 | 59 | 106 | 7.88e-11 | 53.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005371.01.T01 | AT4G09890 | 55.914 | 93 | 27 | 4 | 1 | 86 | 2 | 87 | 1.28e-24 | 88.6 |
| MsG0180005371.01.T01 | AT5G11970 | 54.545 | 44 | 20 | 0 | 42 | 85 | 60 | 103 | 6.35e-12 | 56.6 |
| MsG0180005371.01.T01 | AT3G62640 | 37.209 | 86 | 44 | 2 | 10 | 85 | 25 | 110 | 6.10e-11 | 54.3 |
| MsG0180005371.01.T01 | AT1G72720 | 47.170 | 53 | 28 | 0 | 34 | 86 | 74 | 126 | 8.03e-11 | 54.3 |
Find 16 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACCACTTCCTTTATCACT+TGG | 0.503804 | 1:+90925059 | None:intergenic |
| AAGTTCAGCGATGTTCGGTA+TGG | 0.569967 | 1:-90924918 | MsG0180005371.01.T01:CDS |
| AATCAAGTCCTATAATGTGT+TGG | 0.586946 | 1:-90924997 | MsG0180005371.01.T01:CDS |
| AGAACAAGTTCAGCGATGTT+CGG | 0.522118 | 1:-90924923 | MsG0180005371.01.T01:CDS |
| CAAATGATTCAGAGCTTAAG+AGG | 0.471110 | 1:-90925028 | MsG0180005371.01.T01:CDS |
| CTGAATTTGGATACAGTGAA+AGG | 0.593305 | 1:-90925106 | MsG0180005371.01.T01:CDS |
| GGACCAAGTGATAAAGGAAG+TGG | 0.529951 | 1:-90925062 | MsG0180005371.01.T01:CDS |
| GTCCTATAATGTGTTGGCAG+TGG | 0.532233 | 1:-90924991 | MsG0180005371.01.T01:CDS |
| GTGTAAGAAACAGCTTCAAG+TGG | 0.577880 | 1:-90924950 | MsG0180005371.01.T01:CDS |
| GTGTAAGAACAAGAGTATTC+AGG | 0.369295 | 1:+90925128 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATCAAGTCCTATAATGTGT+TGG | - | Chr1:90925063-90925082 | MsG0180005371.01.T01:CDS | 30.0% | |
| TCTTGTTCTTACACTGAATT+TGG | - | Chr1:90924941-90924960 | MsG0180005371.01.T01:CDS | 30.0% | |
| AAACCACTTCCTTTATCACT+TGG | + | Chr1:90925004-90925023 | None:intergenic | 35.0% | |
| CAAATGATTCAGAGCTTAAG+AGG | - | Chr1:90925032-90925051 | MsG0180005371.01.T01:CDS | 35.0% | |
| CTGAATTTGGATACAGTGAA+AGG | - | Chr1:90924954-90924973 | MsG0180005371.01.T01:CDS | 35.0% | |
| TTCAATGGACCAAGTGATAA+AGG | - | Chr1:90924992-90925011 | MsG0180005371.01.T01:CDS | 35.0% | |
| AGAACAAGTTCAGCGATGTT+CGG | - | Chr1:90925137-90925156 | MsG0180005371.01.T01:CDS | 40.0% | |
| GTGTAAGAAACAGCTTCAAG+TGG | - | Chr1:90925110-90925129 | MsG0180005371.01.T01:CDS | 40.0% | |
| TTCCACTGCCAACACATTAT+AGG | + | Chr1:90925074-90925093 | None:intergenic | 40.0% | |
| AAGTTCAGCGATGTTCGGTA+TGG | - | Chr1:90925142-90925161 | MsG0180005371.01.T01:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 90924911 | 90925171 | 90924911 | ID=MsG0180005371.01;Name=MsG0180005371.01 |
| Chr1 | mRNA | 90924911 | 90925171 | 90924911 | ID=MsG0180005371.01.T01;Parent=MsG0180005371.01;Name=MsG0180005371.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|86 |
| Chr1 | exon | 90924911 | 90925171 | 90924911 | ID=MsG0180005371.01.T01:exon:38120;Parent=MsG0180005371.01.T01 |
| Chr1 | CDS | 90924911 | 90925171 | 90924911 | ID=MsG0180005371.01.T01:cds;Parent=MsG0180005371.01.T01 |
| Gene Sequence |
>MsG0180005371.01.T01 ATGGAGAAAAGCAAGTCATTTCCTGAATACTCTTGTTCTTACACTGAATTTGGATACAGTGAAAGGTCCAACTCATATAATTTCAATGGACCAAGTGATAAAGGAAGTGGTTTTTCTGCATCAAATGATTCAGAGCTTAAGAGGAAGAAGAGAATCAAGTCCTATAATGTGTTGGCAGTGGAAGGAAAGCTTAAAAGTAGTGTAAGAAACAGCTTCAAGTGGATCAAGAACAAGTTCAGCGATGTTCGGTATGGCGTGTGA |
| Protein sequence |
>MsG0180005371.01.T01 MEKSKSFPEYSCSYTEFGYSERSNSYNFNGPSDKGSGFSASNDSELKRKKRIKSYNVLAVEGKLKSSVRNSFKWIKNKFSDVRYGV* |
