AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048764.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048764.01.T01 MTR_2g105760 97.872 94 2 0 1 94 2230 2323 2.89e-56 187
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 17 sgRNAs with CRISPR-Local

Find 17 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGAGCTTCTAATCAAAGTA+TGG 0.287990 contig428end:-28418 MsG0080048764.01.T01:CDS
CGACAAAAGTTTCTGCAAAA+AGG 0.314037 contig428end:-28305 MsG0080048764.01.T01:CDS
TATTCGCAACTCTCTCTTTG+TGG 0.404962 contig428end:+28448 MsG0080048764.01.T01:intergenic
TTTCCTCATCTGTTGCAAAC+GGG 0.450132 contig428end:-28278 MsG0080048764.01.T01:CDS
TGGGTGCTGAAATTTCAAAA+AGG 0.515878 contig428end:-28398 MsG0080048764.01.T01:CDS
AGAACTGCTCATATCCCCTC+TGG 0.517730 contig428end:+28503 MsG0080048764.01.T01:intergenic
GTTTGCAACAGATGAGGAAA+AGG 0.532827 contig428end:+28281 MsG0080048764.01.T01:intergenic
ATGTTTGATATCTACGAAGA+AGG 0.548233 contig428end:-28337 MsG0080048764.01.T01:CDS
GTTACTGCTTCCTCAATGAA+AGG 0.581593 contig428end:-28474 MsG0080048764.01.T01:CDS
AAACCACCTAAGAAAAGAAG+TGG 0.583304 contig428end:-28249 MsG0080048764.01.T01:CDS
AGAGCTTCTAATCAAAGTAT+GGG 0.589838 contig428end:-28417 MsG0080048764.01.T01:CDS
ATTAGTAAAGAGCAAAGATG+AGG 0.607022 contig428end:+28219 MsG0080048765.01.T01:intergenic
TTTGTGGCATCCTTTCATTG+AGG 0.623333 contig428end:+28464 MsG0080048765.01.T01:intergenic
AACTGGCTATGCATTCCAGA+GGG 0.627352 contig428end:-28518 MsG0080048765.01.T01:CDS
ATTCCCGTTTGCAACAGATG+AGG 0.634564 contig428end:+28275 MsG0080048765.01.T01:intergenic
AAACTGGCTATGCATTCCAG+AGG 0.672098 contig428end:-28519 MsG0080048765.01.T01:CDS
ACTGGCTATGCATTCCAGAG+GGG 0.727251 contig428end:-28517 MsG0080048765.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGAGCTTCTAATCAAAGTAT+GGG - contig428end:28299-28318 MsG0080048764.01.T01:CDS 30.0%
ATGTTTGATATCTACGAAGA+AGG - contig428end:28379-28398 MsG0080048764.01.T01:CDS 30.0%
ATTAGTAAAGAGCAAAGATG+AGG + contig428end:28500-28519 MsG0080048764.01.T01:intergenic 30.0%
TAGAGCTTCTAATCAAAGTA+TGG - contig428end:28298-28317 MsG0080048764.01.T01:CDS 30.0%
! AGTTTACCACTTCTTTTCTT+AGG + contig428end:28476-28495 MsG0080048764.01.T01:intergenic 30.0%
AAACCACCTAAGAAAAGAAG+TGG - contig428end:28467-28486 MsG0080048764.01.T01:CDS 35.0%
CGACAAAAGTTTCTGCAAAA+AGG - contig428end:28411-28430 MsG0080048764.01.T01:CDS 35.0%
! TTACCACTTCTTTTCTTAGG+TGG + contig428end:28473-28492 MsG0080048764.01.T01:intergenic 35.0%
! TTTTCCTCATCTGTTGCAAA+CGG - contig428end:28437-28456 MsG0080048764.01.T01:CDS 35.0%
!! TGGGTGCTGAAATTTCAAAA+AGG - contig428end:28318-28337 MsG0080048764.01.T01:CDS 35.0%
GTTACTGCTTCCTCAATGAA+AGG - contig428end:28242-28261 MsG0080048764.01.T01:CDS 40.0%
GTTTGCAACAGATGAGGAAA+AGG + contig428end:28438-28457 MsG0080048764.01.T01:intergenic 40.0%
TATTCGCAACTCTCTCTTTG+TGG + contig428end:28271-28290 MsG0080048764.01.T01:intergenic 40.0%
TTTCCTCATCTGTTGCAAAC+GGG - contig428end:28438-28457 MsG0080048764.01.T01:CDS 40.0%
TTTGTGGCATCCTTTCATTG+AGG + contig428end:28255-28274 MsG0080048764.01.T01:intergenic 40.0%
ATTCCCGTTTGCAACAGATG+AGG + contig428end:28444-28463 MsG0080048764.01.T01:intergenic 45.0%
AGAACTGCTCATATCCCCTC+TGG + contig428end:28216-28235 MsG0080048764.01.T01:intergenic 50.0%
Chromosome Type Strat End Strand Name
contig428end gene 28206 28532 28206 ID=MsG0080048764.01;Name=MsG0080048764.01
contig428end mRNA 28206 28532 28206 ID=MsG0080048764.01.T01;Parent=MsG0080048764.01;Name=MsG0080048764.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|108
contig428end exon 28206 28532 28206 ID=MsG0080048764.01.T01:exon:22827;Parent=MsG0080048764.01.T01
contig428end CDS 28206 28532 28206 ID=MsG0080048764.01.T01:cds;Parent=MsG0080048764.01.T01
Gene Sequence

>MsG0080048764.01.T01

ATGCATTCCAGAGGGGATATGAGCAGTTCTACAGAAGTTACTGCTTCCTCAATGAAAGGATGCCACAAAGAGAGAGTTGCGAATAATCAAGATAGAGCTTCTAATCAAAGTATGGGTGCTGAAATTTCAAAAAGGAAACAGCAGCGTGAAGCTGTTGATGCTATTCTTAACTCATGTTTGATATCTACGAAGAAGGATGAGAGATCGACAAAAGTTTCTGCAAAAAGGTCCTTTTCCTCATCTGTTGCAAACGGGAATATGAAACCACCTAAGAAAAGAAGTGGTAAACTTCCTCATCTTTGCTCTTTACTAATATTGATGATTTAG

Protein sequence

>MsG0080048764.01.T01

MHSRGDMSSSTEVTASSMKGCHKERVANNQDRASNQSMGAEISKRKQQREAVDAILNSCLISTKKDERSTKVSAKRSFSSSVANGNMKPPKKRSGKLPHLCSLLILMI*