Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005898.01.T01 | A0A4D9BJ97 | 51.464 | 239 | 74 | 4 | 33 | 229 | 71 | 309 | 1.30e-59 | 199 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048085.01 | MsG0180005898.01 | 0.819537 | 1.102426e-52 | 8.507363e-50 |
| MsG0080048273.01 | MsG0180005898.01 | 0.811826 | 5.765436e-51 | 3.605526e-48 |
| MsG0080048274.01 | MsG0180005898.01 | 0.817670 | 2.924028e-52 | 2.143035e-49 |
| MsG0080048887.01 | MsG0180005898.01 | 0.866082 | 3.696868e-65 | 1.230427e-61 |
| MsG0080048918.01 | MsG0180005898.01 | 0.840858 | 6.751534e-58 | 9.743559e-55 |
| MsG0080048987.01 | MsG0180005898.01 | 0.804449 | 2.148625e-49 | 1.108136e-46 |
| MsG0080049008.01 | MsG0180005898.01 | -0.820424 | 6.909544e-53 | 5.465336e-50 |
| MsG0080049023.01 | MsG0180005898.01 | 0.810346 | 1.206617e-50 | 7.255463e-48 |
| MsG0080049024.01 | MsG0180005898.01 | 0.859236 | 4.757292e-63 | 1.247637e-59 |
| MsG0080049038.01 | MsG0180005898.01 | 0.818451 | 1.947353e-52 | 1.458337e-49 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005898.01.T01 | MTR_1g109080 | 97.959 | 98 | 2 | 0 | 33 | 130 | 82 | 179 | 2.03e-62 | 198 |
| MsG0180005898.01.T01 | MTR_1g109080 | 98.387 | 62 | 1 | 0 | 168 | 229 | 282 | 343 | 1.08e-33 | 124 |
Find 56 sgRNAs with CRISPR-Local
Find 178 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACTCTGTCAAGTTGCCAA+CGG | 0.663733 | 1:+97258793 | None:intergenic |
| AAAGATATGCTTGATGGTAT+AGG | 0.428767 | 1:-97259223 | MsG0180005898.01.T01:CDS |
| AAAGATCAAAGTCTTTGTGT+GGG | 0.620237 | 1:-97258761 | MsG0180005898.01.T01:intron |
| AACTTGAGTGAGAGGATCTG+TGG | 0.626725 | 1:+97257274 | None:intergenic |
| AAGACTTTGATCTTTCACCC+TGG | 0.602977 | 1:+97258769 | None:intergenic |
| ACCGGATGCTATCAGTTTGG+AGG | 0.588400 | 1:-97257343 | MsG0180005898.01.T01:CDS |
| ACCTCCAAACTGATAGCATC+CGG | 0.541278 | 1:+97257342 | None:intergenic |
| AGAATCTTTATATCAGTTCT+AGG | 0.249354 | 1:-97259067 | MsG0180005898.01.T01:CDS |
| AGATTTCTTCAGCTAGCTCC+TGG | 0.459845 | 1:-97258959 | MsG0180005898.01.T01:CDS |
| AGGAAGAGTAATGCTCTGGT+AGG | 0.605861 | 1:+97257096 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGATCAAAGTCTTTGTGT+GGG | - | Chr1:97257534-97257553 | MsG0180005898.01.T01:intron | 30.0% | |
| AATGTTTGTAATCGATCATC+TGG | - | Chr1:97258485-97258504 | MsG0180005898.01.T01:intron | 30.0% | |
| ATATTACATCACAACTCACT+AGG | + | Chr1:97257437-97257456 | None:intergenic | 30.0% | |
| ATCATAGCCTTTATAATTCC+AGG | - | Chr1:97257980-97257999 | MsG0180005898.01.T01:intron | 30.0% | |
| ATGCTCATTTCTTTATCATC+TGG | + | Chr1:97257180-97257199 | None:intergenic | 30.0% | |
| GACAATAAAGATATGCTTGA+TGG | - | Chr1:97257066-97257085 | MsG0180005898.01.T01:three_prime_UTR | 30.0% | |
| GCATTTCATGTGAAGTTTAA+AGG | - | Chr1:97257472-97257491 | MsG0180005898.01.T01:intron | 30.0% | |
| GTAATGGTTATATCACACAA+AGG | + | Chr1:97257850-97257869 | None:intergenic | 30.0% | |
| GTCCAATAGAGTTGTATAAT+CGG | + | Chr1:97258911-97258930 | None:intergenic | 30.0% | |
| TAGAGAAGTCAAATTTAGGA+GGG | - | Chr1:97257792-97257811 | MsG0180005898.01.T01:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 97257000 | 97259317 | 97257000 | ID=MsG0180005898.01;Name=MsG0180005898.01 |
| Chr1 | mRNA | 97257000 | 97259317 | 97257000 | ID=MsG0180005898.01.T01;Parent=MsG0180005898.01;Name=MsG0180005898.01.T01;_AED=0.37;_eAED=0.47;_QI=0|0|0.33|1|0.5|0.33|3|205|229 |
| Chr1 | exon | 97257000 | 97257412 | 97257000 | ID=MsG0180005898.01.T01:exon:50659;Parent=MsG0180005898.01.T01 |
| Chr1 | exon | 97258762 | 97258830 | 97258762 | ID=MsG0180005898.01.T01:exon:50658;Parent=MsG0180005898.01.T01 |
| Chr1 | exon | 97258905 | 97259317 | 97258905 | ID=MsG0180005898.01.T01:exon:50657;Parent=MsG0180005898.01.T01 |
| Chr1 | CDS | 97258905 | 97259317 | 97258905 | ID=MsG0180005898.01.T01:cds;Parent=MsG0180005898.01.T01 |
| Chr1 | CDS | 97258762 | 97258830 | 97258762 | ID=MsG0180005898.01.T01:cds;Parent=MsG0180005898.01.T01 |
| Chr1 | CDS | 97257205 | 97257412 | 97257205 | ID=MsG0180005898.01.T01:cds;Parent=MsG0180005898.01.T01 |
| Chr1 | three_prime_UTR | 97257000 | 97257204 | 97257000 | ID=MsG0180005898.01.T01:three_prime_utr;Parent=MsG0180005898.01.T01 |
| Gene Sequence |
>MsG0180005898.01.T01 ATGATTATGCATGCAAAAGTTTTAGAAATAGAAACTCTTACTGAATTAACTTTCTATTGTTGTGTTGACAATAAAGATATGCTTGATGGTATAGGTGAAGAAACATTGGAAAATTTTAAGGAAGATGGAGAAAGAAGTGCTATCTATGATTTTCTTTATCCTGATAAAGAGCTTCTTCCAGATGATAAAGAAATGAGCATATTTGATCACCTTGAAGAGCTTCGACAAAGAATCTTTATATCAGTTCTAGGAGTTGGAGGAAGCATTTTGGGATGCTTTGCATTTTCAAAAGATTTGGTGAGGCTTCTTGAAGCTCCTGTTCAATCAGAGGGTGTCAGATTTCTTCAGCTAGCTCCTGGTGAATTTTTCTTCACCACTTTAAAGGTAAATTTTTTACTCAAAATATCTCCGGGTTTAAAGGTGCTATACCGTTGGCAACTTGACAGAGTTTTCCAGGGTGAAAGATCAAAGTCTTTGTGTGGGTACCGATTATACAACTCTATTGGACAACTTGGATTGGTTACTGGAGACCGGATGCTATCAGTTTGGAGGTACGTTGTAGTCGGAGCAGTAGTGGCAGCTGCTATAGTTACGCCATCCACAGATCCTCTCACTCAAGTTCTTCTAGCAGCACCATTATTAGGTCTTTACCTAGGTGGTGCATGGATGGTCAAACTTGCTGGGCGTTAA |
| Protein sequence |
>MsG0180005898.01.T01 MIMHAKVLEIETLTELTFYCCVDNKDMLDGIGEETLENFKEDGERSAIYDFLYPDKELLPDDKEMSIFDHLEELRQRIFISVLGVGGSILGCFAFSKDLVRLLEAPVQSEGVRFLQLAPGEFFFTTLKVNFLLKISPGLKVLYRWQLDRVFQGERSKSLCGYRLYNSIGQLGLVTGDRMLSVWRYVVVGAVVAAAIVTPSTDPLTQVLLAAPLLGLYLGGAWMVKLAGR* |
