Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007043.01.T01 | XP_003593922.1 | 86.405 | 331 | 15 | 2 | 9 | 309 | 6 | 336 | 0 | 573 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280007031.01 | MsG0280007043.01 | 0.913881 | 3.678476e-84 | 8.889586e-80 |
| MsG0280007043.01 | MsG0280008295.01 | 0.800788 | 1.222873e-48 | 5.744533e-46 |
| MsG0280007043.01 | MsG0280010784.01 | 0.807865 | 4.101558e-50 | 2.309998e-47 |
| MsG0280007043.01 | MsG0580025328.01 | 0.820624 | 6.216755e-53 | 4.943991e-50 |
| MsG0280007043.01 | MsG0680031538.01 | 0.804400 | 2.200191e-49 | 1.133269e-46 |
| MsG0280007043.01 | MsG0880042743.01 | 0.804986 | 1.660283e-49 | 8.682294e-47 |
| MsG0280007043.01 | MsG0880043835.01 | 0.814133 | 1.799187e-51 | 1.197295e-48 |
| MsG0280007043.01 | MsG0880044346.01 | 0.809487 | 1.846757e-50 | 1.085679e-47 |
| MsG0280007043.01 | MsG0880046209.01 | 0.800080 | 1.704328e-48 | 7.864124e-46 |
| MsG0280007043.01 | MsG0880046395.01 | 0.810592 | 1.067703e-50 | 6.462447e-48 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007043.01.T01 | MTR_2g019370 | 86.405 | 331 | 15 | 2 | 9 | 309 | 6 | 336 | 0.0 | 573 |
| MsG0280007043.01.T01 | MTR_4g096840 | 61.280 | 328 | 94 | 4 | 9 | 305 | 7 | 332 | 2.15e-142 | 405 |
| MsG0280007043.01.T01 | MTR_2g070870 | 55.140 | 321 | 106 | 5 | 15 | 305 | 12 | 324 | 1.46e-122 | 354 |
| MsG0280007043.01.T01 | MTR_8g461330 | 52.244 | 312 | 118 | 3 | 25 | 305 | 21 | 332 | 1.32e-108 | 319 |
| MsG0280007043.01.T01 | MTR_1g086550 | 45.048 | 313 | 134 | 4 | 30 | 304 | 19 | 331 | 8.40e-87 | 264 |
| MsG0280007043.01.T01 | MTR_4g123020 | 41.538 | 325 | 146 | 8 | 16 | 303 | 1 | 318 | 1.06e-75 | 235 |
| MsG0280007043.01.T01 | MTR_2g033270 | 40.584 | 308 | 139 | 7 | 31 | 304 | 19 | 316 | 6.69e-65 | 207 |
| MsG0280007043.01.T01 | MTR_2g102570 | 29.179 | 329 | 180 | 9 | 11 | 300 | 36 | 350 | 5.00e-32 | 122 |
| MsG0280007043.01.T01 | MTR_5g055680 | 27.653 | 311 | 173 | 10 | 24 | 295 | 40 | 337 | 3.94e-30 | 117 |
| MsG0280007043.01.T01 | MTR_5g055900 | 27.331 | 311 | 174 | 11 | 24 | 295 | 40 | 337 | 1.25e-28 | 113 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007043.01.T01 | AT1G30040 | 54.769 | 325 | 112 | 4 | 13 | 306 | 12 | 332 | 2.78e-124 | 359 |
| MsG0280007043.01.T01 | AT2G34555 | 50.915 | 328 | 123 | 4 | 8 | 305 | 7 | 326 | 1.03e-110 | 325 |
| MsG0280007043.01.T01 | AT1G78440 | 51.475 | 305 | 117 | 3 | 31 | 305 | 18 | 321 | 3.25e-107 | 315 |
| MsG0280007043.01.T01 | AT1G30040 | 51.695 | 236 | 84 | 3 | 13 | 222 | 12 | 243 | 4.93e-81 | 246 |
| MsG0280007043.01.T01 | AT1G02400 | 41.883 | 308 | 141 | 5 | 31 | 304 | 24 | 327 | 7.42e-76 | 235 |
| MsG0280007043.01.T01 | AT1G47990 | 40.184 | 326 | 146 | 10 | 17 | 303 | 1 | 316 | 1.69e-65 | 209 |
| MsG0280007043.01.T01 | AT1G02400 | 40.183 | 219 | 99 | 3 | 32 | 222 | 25 | 239 | 1.33e-49 | 166 |
| MsG0280007043.01.T01 | AT1G15550 | 29.755 | 326 | 178 | 10 | 13 | 299 | 40 | 353 | 2.64e-33 | 125 |
| MsG0280007043.01.T01 | AT5G08640 | 29.524 | 315 | 169 | 10 | 14 | 286 | 22 | 325 | 1.16e-30 | 118 |
| MsG0280007043.01.T01 | AT5G08640 | 29.524 | 315 | 169 | 10 | 14 | 286 | 22 | 325 | 1.16e-30 | 118 |
Find 74 sgRNAs with CRISPR-Local
Find 201 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAAGCAGCATTGAATGGA+AGG | 0.537153 | 2:-9825817 | MsG0280007043.01.T01:CDS |
| AACCATTACCCTGAGGACAC+CGG | 0.623538 | 2:+9827592 | None:intergenic |
| AAGGAATTTGCTTGGGTTTG+GGG | 0.471960 | 2:-9825798 | MsG0280007043.01.T01:CDS |
| AAGTAAGAAATTATTAATGT+TGG | 0.490220 | 2:+9826910 | None:intergenic |
| AAGTGTAAAGCATAGGGTTT+TGG | 0.313870 | 2:-9825050 | MsG0280007043.01.T01:CDS |
| AATGCTGCTTGTTGCACCTC+AGG | 0.352397 | 2:+9825827 | None:intergenic |
| ACTATAAGAGTCTTTGCTTC+CGG | 0.371721 | 2:+9826824 | None:intergenic |
| ACTTGCTTAAACACATTCCT+TGG | 0.452376 | 2:+9825906 | None:intergenic |
| ACTTGTTAGAGATAACATCA+GGG | 0.698450 | 2:+9826586 | None:intergenic |
| AGAAAGCAATGTACAATTCA+AGG | 0.429933 | 2:-9824892 | MsG0280007043.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATTATATAGTGACCCATC+TGG | + | Chr2:9825365-9825384 | None:intergenic | 30.0% | |
| ACATTGAAAAGGAAAAACAG+GGG | + | Chr2:9825961-9825980 | None:intergenic | 30.0% | |
| AGAAAGCAATGTACAATTCA+AGG | - | Chr2:9827558-9827577 | MsG0280007043.01.T01:intron | 30.0% | |
| CAACATTGAAAAGGAAAAAC+AGG | + | Chr2:9825963-9825982 | None:intergenic | 30.0% | |
| CCATAAACTGAAAATCTCAT+CGG | + | Chr2:9826008-9826027 | None:intergenic | 30.0% | |
| CCCCACTTCAAAAATTTAAA+TGG | + | Chr2:9827287-9827306 | None:intergenic | 30.0% | |
| GAGAAGAAAAATAGAGATGA+AGG | + | Chr2:9825440-9825459 | None:intergenic | 30.0% | |
| GATAGAATGGAATTCCAATT+CGG | - | Chr2:9825043-9825062 | MsG0280007043.01.T01:CDS | 30.0% | |
| GATGTTAACCAATGTTCTTA+TGG | - | Chr2:9824978-9824997 | MsG0280007043.01.T01:CDS | 30.0% | |
| GGTTATCAAACATTCTAACT+TGG | - | Chr2:9827319-9827338 | MsG0280007043.01.T01:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 9824844 | 9827628 | 9824844 | ID=MsG0280007043.01;Name=MsG0280007043.01 |
| Chr2 | mRNA | 9824844 | 9827628 | 9824844 | ID=MsG0280007043.01.T01;Parent=MsG0280007043.01;Name=MsG0280007043.01.T01;_AED=0.33;_eAED=0.33;_QI=0|0|0|0.8|1|1|5|0|309 |
| Chr2 | exon | 9827601 | 9827628 | 9827601 | ID=MsG0280007043.01.T01:exon:2239;Parent=MsG0280007043.01.T01 |
| Chr2 | exon | 9826790 | 9826932 | 9826790 | ID=MsG0280007043.01.T01:exon:2238;Parent=MsG0280007043.01.T01 |
| Chr2 | exon | 9826549 | 9826715 | 9826549 | ID=MsG0280007043.01.T01:exon:2237;Parent=MsG0280007043.01.T01 |
| Chr2 | exon | 9825673 | 9826012 | 9825673 | ID=MsG0280007043.01.T01:exon:2236;Parent=MsG0280007043.01.T01 |
| Chr2 | exon | 9824844 | 9825095 | 9824844 | ID=MsG0280007043.01.T01:exon:2235;Parent=MsG0280007043.01.T01 |
| Chr2 | CDS | 9827601 | 9827628 | 9827601 | ID=MsG0280007043.01.T01:cds;Parent=MsG0280007043.01.T01 |
| Chr2 | CDS | 9826790 | 9826932 | 9826790 | ID=MsG0280007043.01.T01:cds;Parent=MsG0280007043.01.T01 |
| Chr2 | CDS | 9826549 | 9826715 | 9826549 | ID=MsG0280007043.01.T01:cds;Parent=MsG0280007043.01.T01 |
| Chr2 | CDS | 9825673 | 9826012 | 9825673 | ID=MsG0280007043.01.T01:cds;Parent=MsG0280007043.01.T01 |
| Chr2 | CDS | 9824844 | 9825095 | 9824844 | ID=MsG0280007043.01.T01:cds;Parent=MsG0280007043.01.T01 |
| Gene Sequence |
>MsG0280007043.01.T01 ATGTGTCTGTCTAACCGGTGTCCTCAGGCCAACATTAATAATTTCTTACTTGTCAAGTCATGCAAACCATCAACAACTTTGTTGAATGGTATTCCTGTCGTCGACCTTGCAGACCCGGAAGCAAAGACTCTTATAGTGAAGGCTTGTAAGGAATTTGGATTCTTCAAAGTGTCAGAGAAAGACAGAGCTGGTCCTCCTGATCCTTTTGGTTATGGAAGTAAGAGAATTGGTTCAAATGGTGATGTTGGTTGGGTTGAATATATCCTTCTTAATACTAACCCTGATGTTATCTCTAACAAGTCTCTTTCCTTTTACCGTGAAAATCGACAAAATCTAAGGTCTGCTGTGGAAGATTATATTGCTGCAATGAAGAAAATGTGCTGTCTAGTGTTGGAATTAATGGCTGATGGTTTGGGATTGAGCCAAGGAATGTGTTTAAGCAAGTTATTGAAAGATGAGAAAAGTGATTCTTGTTTCAGAATTAACCATTACCCACCGTGCCCTGAGGTGCAACAAGCAGCATTGAATGGAAGGAATTTGCTTGGGTTTGGGGAGCATACAGACCCACAAGTCATTTCTGTCTTGAGATCTAATAGCACATCAGGACTCCAAATCTGTCTCACTGATGGAACTTGGGTTTCAGTTCCACCTGATCACACTTCTTTTTCATCAATGTTGGTATTGACTAATGGTAGGTTTAAAAGTGTAAAGCATAGGGTTTTGGCTGACACAACAAAGTCAAGGTTGTCGATGATATACTTTGGAGGACCACCCTTGAGTGAAAAGATAGTGCCTTTACCATCTTTAATGTTAAAAAAAGAAGAAAGTTTGTACAAAGAGTTCACGTGGTTGGAGTACAAGAAAGCAATGTACAATTCAAGGCTGGCTGATTATAGACTTGGGCCTTTTGAAAAATCTTATGGCAAATGA |
| Protein sequence |
>MsG0280007043.01.T01 MCLSNRCPQANINNFLLVKSCKPSTTLLNGIPVVDLADPEAKTLIVKACKEFGFFKVSEKDRAGPPDPFGYGSKRIGSNGDVGWVEYILLNTNPDVISNKSLSFYRENRQNLRSAVEDYIAAMKKMCCLVLELMADGLGLSQGMCLSKLLKDEKSDSCFRINHYPPCPEVQQAALNGRNLLGFGEHTDPQVISVLRSNSTSGLQICLTDGTWVSVPPDHTSFSSMLVLTNGRFKSVKHRVLADTTKSRLSMIYFGGPPLSEKIVPLPSLMLKKEESLYKEFTWLEYKKAMYNSRLADYRLGPFEKSYGK* |
