AlfalfaGEDB | MsG0680031538.01

M. sativa cultivar ZhongmuNo.1 / MsG0680031538.01

KEGG: K02183
GO: GO:0005509
PFAM: PF13499

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031538.01.T01	KAF8053302.1	100	149	0	0	1	149	1	149	3.15E-103	303

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031538.01.T01	P59220	100	149	0	0	1	149	1	149	2.35E-105	300

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031538.01.T01	A0A218X1R4	100.000	149	0	0	1	149	65	213	8.89e-104	305

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047825.01	MsG0680031538.01	0.846801	1.728756e-59	3.010766e-56
MsG0080047912.01	MsG0680031538.01	0.809131	2.201887e-50	1.282282e-47
MsG0080048177.01	MsG0680031538.01	0.842427	2.603065e-58	3.946009e-55
MsG0080048178.01	MsG0680031538.01	0.828151	1.054123e-54	1.039102e-51
MsG0080048210.01	MsG0680031538.01	0.805063	1.599400e-49	8.380639e-47
MsG0080048656.01	MsG0680031538.01	0.829208	5.853339e-55	5.951115e-52
MsG0080048770.01	MsG0680031538.01	0.816303	5.929713e-52	4.186392e-49
MsG0080048830.01	MsG0680031538.01	0.812744	3.634201e-51	2.329401e-48
MsG0080048968.01	MsG0680031538.01	0.837232	5.870537e-57	7.576890e-54
MsG0180000153.01	MsG0680031538.01	0.808802	2.589482e-50	1.494709e-47

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PPI

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Gene1	Gene2	Type
MsG0180001953.01	MsG0680031538.01	PPI
MsG0280011105.01	MsG0680031538.01	PPI
MsG0480024016.01	MsG0680031538.01	PPI
MsG0580024305.01	MsG0680031538.01	PPI
MsG0580024499.01	MsG0680031538.01	PPI
MsG0680031459.01	MsG0680031538.01	PPI
MsG0680031538.01	MsG0580028980.01	PPI
MsG0680031538.01	MsG0380015686.01	PPI
MsG0680031538.01	MsG0580024478.01	PPI
MsG0680031538.01	MsG0380015687.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031538.01.T01	MTR_6g025320	100.000	149	0	0	1	149	1	149	2.29e-106	300
MsG0680031538.01.T01	MTR_5g088320	99.329	149	1	0	1	149	1	149	8.74e-106	298
MsG0680031538.01.T01	MTR_7g087610	98.658	149	2	0	1	149	1	149	2.35e-104	295
MsG0680031538.01.T01	MTR_1g041285	78.378	148	32	0	1	148	1	148	3.51e-83	241
MsG0680031538.01.T01	MTR_1g041285	47.692	65	34	0	85	149	12	76	5.42e-12	60.1
MsG0680031538.01.T01	MTR_1g076650	77.703	148	33	0	1	148	1	148	5.81e-83	241
MsG0680031538.01.T01	MTR_1g076650	45.070	71	36	1	82	149	6	76	2.91e-11	58.2
MsG0680031538.01.T01	MTR_0168s0120	100.000	113	0	0	37	149	1	113	1.18e-78	228
MsG0680031538.01.T01	MTR_0168s0120	45.455	77	41	1	1	76	37	113	5.83e-14	64.3
MsG0680031538.01.T01	MTR_6g025320	100.000	113	0	0	37	149	1	113	1.18e-78	228

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031538.01.T01	AT3G43810	100.000	148	0	0	1	148	1	148	1.28e-106	303
MsG0680031538.01.T01	AT3G43810	45.455	77	41	1	73	149	1	76	4.86e-13	64.3
MsG0680031538.01.T01	AT3G43810	100.000	149	0	0	1	149	1	149	2.40e-106	300
MsG0680031538.01.T01	AT2G27030	99.329	149	1	0	1	149	1	149	4.05e-106	300
MsG0680031538.01.T01	AT2G27030	44.304	79	43	1	1	78	73	151	1.81e-13	64.7
MsG0680031538.01.T01	AT5G21274	99.329	149	1	0	1	149	1	149	7.59e-106	298
MsG0680031538.01.T01	AT3G56800	99.329	149	1	0	1	149	1	149	9.05e-106	298
MsG0680031538.01.T01	AT2G27030	99.329	149	1	0	1	149	1	149	9.05e-106	298
MsG0680031538.01.T01	AT2G41110	99.329	149	1	0	1	149	1	149	9.05e-106	298
MsG0680031538.01.T01	AT5G37780	97.315	149	4	0	1	149	1	149	2.46e-104	295

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Find 62 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTATGAGGAATTCGTTA+AGG	0.465882	6:-21910139	MsG0680031538.01.T01:CDS
AAGAAGAAGAAAAGCGACGA+TGG	0.585072	6:-21913244	MsG0680031538.01.T01:exon
AAGGAAATGACAATAGGGGA+GGG	0.580835	6:-21910044	MsG0680031538.01.T01:three_prime_UTR
ACCATTCACCAAAATATGAA+TGG	0.512874	6:+21909827	None:intergenic
AGCTTTCAGCTTATTCGATA+AGG	0.414465	6:-21913181	MsG0680031538.01.T01:CDS
AGGAAATGACAATAGGGGAG+GGG	0.569407	6:-21910043	MsG0680031538.01.T01:three_prime_UTR
AGGAGCTGAAAGAGGCGTTT+CGG	0.391958	6:-21910299	MsG0680031538.01.T01:CDS
AGTCCTGCTGCTATTGGTTG+TGG	0.512344	6:-21909928	MsG0680031538.01.T01:three_prime_UTR
AGTCGATGAGATGATCCGTG+AGG	0.760734	6:-21910187	MsG0680031538.01.T01:CDS
AGTTCCTACCATTCATATTT+TGG	0.145819	6:-21909835	MsG0680031538.01.T01:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACTATGAGGAATTCGTTA+AGG	-	Chr6:21912967-21912986	MsG0680031538.01.T01:intron	30.0%
AACGGATCAAAGCAAATAAT+GGG	-	Chr6:21911233-21911252	MsG0680031538.01.T01:intron	30.0%
ACCATTCACCAAAATATGAA+TGG	+	Chr6:21913282-21913301	None:intergenic	30.0%
ATCATAATCTTTATCCTTGC+TGG	+	Chr6:21910969-21910988	None:intergenic	30.0%
ATGACTTTGATTACAAGTGT+TGG	+	Chr6:21910757-21910776	None:intergenic	30.0%
ATGTGGCTTATATACTAGTA+GGG	-	Chr6:21911549-21911568	MsG0680031538.01.T01:intron	30.0%
CCGAATTGTTAGTGTTAAAT+AGG	-	Chr6:21910897-21910916	MsG0680031538.01.T01:intron	30.0%
GATTAAGGAAATGACAATAG+GGG	-	Chr6:21913058-21913077	MsG0680031538.01.T01:intron	30.0%
GTAAGAAGTCATTTGCTTAT+AGG	-	Chr6:21912547-21912566	MsG0680031538.01.T01:intron	30.0%
TATGTGGCTTATATACTAGT+AGG	-	Chr6:21911548-21911567	MsG0680031538.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	21909740	21913388	21909740	ID=MsG0680031538.01;Name=MsG0680031538.01
Chr6	mRNA	21909740	21913388	21909740	ID=MsG0680031538.01.T01;Parent=MsG0680031538.01;Name=MsG0680031538.01.T01;_AED=0.49;_eAED=0.49;_QI=141\|1\|1\|1\|1\|1\|2\|382\|149
Chr6	exon	21909740	21910495	21909740	ID=MsG0680031538.01.T01:exon:13795;Parent=MsG0680031538.01.T01
Chr6	exon	21913172	21913388	21913172	ID=MsG0680031538.01.T01:exon:13794;Parent=MsG0680031538.01.T01
Chr6	five_prime_UTR	21913248	21913388	21913248	ID=MsG0680031538.01.T01:five_prime_utr;Parent=MsG0680031538.01.T01
Chr6	CDS	21913172	21913247	21913172	ID=MsG0680031538.01.T01:cds;Parent=MsG0680031538.01.T01
Chr6	CDS	21910122	21910495	21910122	ID=MsG0680031538.01.T01:cds;Parent=MsG0680031538.01.T01
Chr6	three_prime_UTR	21909740	21910121	21909740	ID=MsG0680031538.01.T01:three_prime_utr;Parent=MsG0680031538.01.T01

Gene Sequence

>MsG0680031538.01.T01

ATGGCAGATCAACTCACAGATGATCAGATCTCTGAGTTCAAGGAAGCTTTCAGCTTATTCGATAAGGATGGCGATGGTTGCATCACAACCAAGGAGCTTGGAACTGTTATGCGTTCATTGGGGCAAAACCCAACTGAGGCAGAGCTTCAGGACATGATCAATGAAGTAGATGCTGATGGGAATGGTACTATTGACTTCCCTGAGTTTCTTAACCTCATGGCAAGGAAGATGAAGGACACCGACTCCGAGGAGGAGCTGAAAGAGGCGTTTCGGGTTTTTGACAAGGATCAGAATGGTTTCATTTCTGCTGCTGAGCTTCGTCATGTGATGACCAACCTTGGCGAGAAGCTCACCGATGAAGAAGTCGATGAGATGATCCGTGAGGCTGATGTTGATGGTGATGGTCAAATAAACTATGAGGAATTCGTTAAGGTCATGATGGCTAAATGA

Protein sequence

>MsG0680031538.01.T01

MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDEMIREADVDGDGQINYEEFVKVMMAK*