Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007274.01.T01 | XP_013462873.1 | 98.707 | 232 | 3 | 0 | 1 | 232 | 89 | 320 | 7.84E-173 | 488 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007274.01.T01 | A0A072V6F0 | 98.707 | 232 | 3 | 0 | 1 | 232 | 89 | 320 | 3.74e-173 | 488 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000983.01 | MsG0280007274.01 | 0.803019 | 4.256008e-49 | 2.115767e-46 |
| MsG0180003858.01 | MsG0280007274.01 | 0.811517 | 6.728836e-51 | 4.174071e-48 |
| MsG0180004099.01 | MsG0280007274.01 | 0.800501 | 1.398991e-48 | 6.524477e-46 |
| MsG0180004754.01 | MsG0280007274.01 | 0.808807 | 2.583046e-50 | 1.491193e-47 |
| MsG0280007274.01 | MsG0280008280.01 | 0.802983 | 4.329643e-49 | 2.150446e-46 |
| MsG0280007274.01 | MsG0280009514.01 | 0.813042 | 3.127640e-51 | 2.020566e-48 |
| MsG0280007274.01 | MsG0280010779.01 | 0.831269 | 1.837145e-55 | 1.983537e-52 |
| MsG0280007274.01 | MsG0280011282.01 | 0.809785 | 1.593393e-50 | 9.442354e-48 |
| MsG0280007274.01 | MsG0380016994.01 | 0.812558 | 3.990474e-51 | 2.545295e-48 |
| MsG0280007274.01 | MsG0380017159.01 | 0.800027 | 1.747186e-48 | 8.050858e-46 |
| MsG0280007274.01 | MsG0580026307.01 | 0.800092 | 1.695264e-48 | 7.824486e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007274.01.T01 | MTR_2g023970 | 98.707 | 232 | 3 | 0 | 1 | 232 | 89 | 320 | 9.49e-177 | 488 |
| MsG0280007274.01.T01 | MTR_4g132450 | 77.682 | 233 | 51 | 1 | 1 | 232 | 102 | 334 | 3.10e-140 | 396 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007274.01.T01 | AT1G11915 | 64.255 | 235 | 80 | 4 | 1 | 232 | 96 | 329 | 9.45e-96 | 283 |
Find 45 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCAAAGATTCACTTGCTTT+TGG | 0.216568 | 2:-12934142 | None:intergenic |
| TATGATCAAGTACCTGAATA+TGG | 0.236309 | 2:+12934232 | MsG0280007274.01.T01:CDS |
| TTGGAATTGGAGCTGGTATT+TGG | 0.243382 | 2:+12934995 | MsG0280007274.01.T01:CDS |
| CTAGTTATGCTAGTGTTTAT+TGG | 0.264957 | 2:+12934185 | MsG0280007274.01.T01:CDS |
| GTGCTAGTGTCTAAACTAAA+TGG | 0.287964 | 2:-12933920 | None:intergenic |
| TTCTCAGCTGCAGGGGCAAT+TGG | 0.337882 | 2:+12934976 | MsG0280007274.01.T01:CDS |
| AAGAAATTGCACAGCACAAT+AGG | 0.351853 | 2:+12934824 | MsG0280007274.01.T01:CDS |
| AGTGTTTATTGGAGGGATAT+TGG | 0.378579 | 2:+12934196 | MsG0280007274.01.T01:CDS |
| TGTTCACAAAACATAGGCTT+TGG | 0.380089 | 2:-12934013 | None:intergenic |
| TTCTGTTACCTGCGATTACA+TGG | 0.382922 | 2:-12934852 | None:intergenic |
| CAAGATGAATCACAATGTTC+AGG | 0.392043 | 2:-12934040 | None:intergenic |
| TCAACTCTAATCCCATATTC+AGG | 0.393312 | 2:-12934244 | None:intergenic |
| GCTGCAGGGGCAATTGGAAT+TGG | 0.402123 | 2:+12934982 | MsG0280007274.01.T01:CDS |
| TTAGAATAGTCTATTATCAT+TGG | 0.412158 | 2:-12935067 | None:intergenic |
| TGAAGACAATGAGTTGTAAC+AGG | 0.413119 | 2:-12934277 | None:intergenic |
| CAAAAGCAAGTGAATCTTTG+AGG | 0.416395 | 2:+12934143 | MsG0280007274.01.T01:CDS |
| TATTCAGGTACTTGATCATA+AGG | 0.447126 | 2:-12934229 | None:intergenic |
| AACTCATTGTCTTCAATGTC+AGG | 0.451481 | 2:+12934285 | MsG0280007274.01.T01:CDS |
| GTTACAGCATTCTCAGCTGC+AGG | 0.468617 | 2:+12934967 | MsG0280007274.01.T01:CDS |
| TTTATGTGTTTGTGTAAGGA+TGG | 0.480529 | 2:+12934358 | MsG0280007274.01.T01:CDS |
| ATGATCAAGTACCTGAATAT+GGG | 0.489019 | 2:+12934233 | MsG0280007274.01.T01:CDS |
| GTTATGCTAGTGTTTATTGG+AGG | 0.492582 | 2:+12934188 | MsG0280007274.01.T01:CDS |
| GGAAAATGTTCACAAAACAT+AGG | 0.507873 | 2:-12934019 | None:intergenic |
| TACTTATGCAGACATTTACC+AGG | 0.520887 | 2:+12934076 | MsG0280007274.01.T01:CDS |
| GGTACTTGATCATAAGGCTC+AGG | 0.533579 | 2:-12934223 | None:intergenic |
| TTATGCTAGTGTTTATTGGA+GGG | 0.537025 | 2:+12934189 | MsG0280007274.01.T01:CDS |
| GTTTGAACTCCACATGTGAC+TGG | 0.542667 | 2:-12935045 | None:intergenic |
| TCAATGTCAGGACACAATGA+AGG | 0.545706 | 2:+12934297 | MsG0280007274.01.T01:CDS |
| AACTCCACTACACATTGTAA+AGG | 0.560482 | 2:+12934382 | MsG0280007274.01.T01:CDS |
| GGGGCAATTGGAATTGGAGC+TGG | 0.569061 | 2:+12934988 | MsG0280007274.01.T01:CDS |
| AGGAAGGTCCATGTAATCGC+AGG | 0.573932 | 2:+12934844 | MsG0280007274.01.T01:CDS |
| TTACAGCATTCTCAGCTGCA+GGG | 0.578537 | 2:+12934968 | MsG0280007274.01.T01:CDS |
| CATACCTTTACAATGTGTAG+TGG | 0.589945 | 2:-12934386 | None:intergenic |
| CAGGACACAATGAAGGGTGG+TGG | 0.591439 | 2:+12934304 | MsG0280007274.01.T01:CDS |
| ATGAAGGGTGGTGGAACTTG+TGG | 0.595707 | 2:+12934313 | MsG0280007274.01.T01:CDS |
| TGGAACTTGTGGATTTGATA+CGG | 0.599283 | 2:+12934324 | MsG0280007274.01.T01:CDS |
| CAATGTCAGGACACAATGAA+GGG | 0.621855 | 2:+12934298 | MsG0280007274.01.T01:CDS |
| TCTAAACTAAATGGTCGCGT+AGG | 0.631352 | 2:-12933911 | None:intergenic |
| TACAGCATTCTCAGCTGCAG+GGG | 0.645559 | 2:+12934969 | MsG0280007274.01.T01:CDS |
| AATTGCACAGCACAATAGGA+AGG | 0.647476 | 2:+12934828 | MsG0280007274.01.T01:CDS |
| GCTACTGCACCAGTCACATG+TGG | 0.647888 | 2:+12935036 | MsG0280007274.01.T01:CDS |
| TTCATGTGGAAATGTGAGGA+TGG | 0.661162 | 2:+12933875 | None:intergenic |
| TCCATTCATGTGGAAATGTG+AGG | 0.693776 | 2:+12933871 | None:intergenic |
| ACTTATGCAGACATTTACCA+GGG | 0.706431 | 2:+12934077 | MsG0280007274.01.T01:CDS |
| TGTCAGGACACAATGAAGGG+TGG | 0.712905 | 2:+12934301 | MsG0280007274.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GACTTTTTTTTTTTATTTTT+TGG | - | Chr2:12934694-12934713 | None:intergenic | 10.0% |
| !! | ACACATAATTCAATAGAATA+GGG | - | Chr2:12934424-12934443 | None:intergenic | 20.0% |
| !! | TACACATAATTCAATAGAAT+AGG | - | Chr2:12934425-12934444 | None:intergenic | 20.0% |
| !! | TACTGAAATAACAAAAACTT+GGG | + | Chr2:12934904-12934923 | MsG0280007274.01.T01:intron | 20.0% |
| !! | TTACTGAAATAACAAAAACT+TGG | + | Chr2:12934903-12934922 | MsG0280007274.01.T01:intron | 20.0% |
| !! | TTAGAATAGTCTATTATCAT+TGG | - | Chr2:12935070-12935089 | None:intergenic | 20.0% |
| !!! | ATGATTAATTGTACTAGTTT+CGG | - | Chr2:12934473-12934492 | None:intergenic | 20.0% |
| !!! | TTATTTTTTGGTACAAAAGA+GGG | - | Chr2:12934682-12934701 | None:intergenic | 20.0% |
| !!! | TTTATTTTTTGGTACAAAAG+AGG | - | Chr2:12934683-12934702 | None:intergenic | 20.0% |
| ! | AAAAAAAAAAAGTCGTTCAC+AGG | + | Chr2:12934700-12934719 | MsG0280007274.01.T01:intron | 25.0% |
| ! | ATATTCTCTCATTCAACTTA+AGG | - | Chr2:12934772-12934791 | None:intergenic | 25.0% |
| ! | TACCTATAATAGTGTTATGT+TGG | - | Chr2:12934608-12934627 | None:intergenic | 25.0% |
| ! | TTGATTGTCTAATGTTTGTT+TGG | + | Chr2:12934795-12934814 | MsG0280007274.01.T01:intron | 25.0% |
| !! | TGATTAATTGTACTAGTTTC+GGG | - | Chr2:12934472-12934491 | None:intergenic | 25.0% |
| ATGATCAAGTACCTGAATAT+GGG | + | Chr2:12934233-12934252 | MsG0280007274.01.T01:CDS | 30.0% | |
| CTAGTTATGCTAGTGTTTAT+TGG | + | Chr2:12934185-12934204 | MsG0280007274.01.T01:CDS | 30.0% | |
| GGAAAATGTTCACAAAACAT+AGG | - | Chr2:12934022-12934041 | None:intergenic | 30.0% | |
| GTCCAACATAACACTATTAT+AGG | + | Chr2:12934603-12934622 | MsG0280007274.01.T01:intron | 30.0% | |
| TATGATCAAGTACCTGAATA+TGG | + | Chr2:12934232-12934251 | MsG0280007274.01.T01:CDS | 30.0% | |
| TATTCAGGTACTTGATCATA+AGG | - | Chr2:12934232-12934251 | None:intergenic | 30.0% | |
| TTATGCTAGTGTTTATTGGA+GGG | + | Chr2:12934189-12934208 | MsG0280007274.01.T01:CDS | 30.0% | |
| TTTATGTGTTTGTGTAAGGA+TGG | + | Chr2:12934358-12934377 | MsG0280007274.01.T01:CDS | 30.0% | |
| ! | TCTCTAGAAATTAAAGCTCA+CGG | - | Chr2:12934527-12934546 | None:intergenic | 30.0% |
| !! | GAGTTTTATGTGTTTGTGTA+AGG | + | Chr2:12934354-12934373 | MsG0280007274.01.T01:CDS | 30.0% |
| AACTCATTGTCTTCAATGTC+AGG | + | Chr2:12934285-12934304 | MsG0280007274.01.T01:CDS | 35.0% | |
| AACTCCACTACACATTGTAA+AGG | + | Chr2:12934382-12934401 | MsG0280007274.01.T01:CDS | 35.0% | |
| AAGAAATTGCACAGCACAAT+AGG | + | Chr2:12934824-12934843 | MsG0280007274.01.T01:CDS | 35.0% | |
| ACACAAATAAAGTCGTTCAC+AGG | + | Chr2:12934651-12934670 | MsG0280007274.01.T01:intron | 35.0% | |
| ACTTATGCAGACATTTACCA+GGG | + | Chr2:12934077-12934096 | MsG0280007274.01.T01:CDS | 35.0% | |
| AGTGTTTATTGGAGGGATAT+TGG | + | Chr2:12934196-12934215 | MsG0280007274.01.T01:CDS | 35.0% | |
| ATATGTGGTCTATTGTTGAC+GGG | - | Chr2:12934576-12934595 | None:intergenic | 35.0% | |
| CAAAAGCAAGTGAATCTTTG+AGG | + | Chr2:12934143-12934162 | MsG0280007274.01.T01:CDS | 35.0% | |
| CAAGATGAATCACAATGTTC+AGG | - | Chr2:12934043-12934062 | None:intergenic | 35.0% | |
| CAATAGACCACATATCAAGA+CGG | + | Chr2:12934581-12934600 | MsG0280007274.01.T01:intron | 35.0% | |
| CATACCTTTACAATGTGTAG+TGG | - | Chr2:12934389-12934408 | None:intergenic | 35.0% | |
| GATATGTGGTCTATTGTTGA+CGG | - | Chr2:12934577-12934596 | None:intergenic | 35.0% | |
| GTGCTAGTGTCTAAACTAAA+TGG | - | Chr2:12933923-12933942 | None:intergenic | 35.0% | |
| GTTATGCTAGTGTTTATTGG+AGG | + | Chr2:12934188-12934207 | MsG0280007274.01.T01:CDS | 35.0% | |
| TACTTATGCAGACATTTACC+AGG | + | Chr2:12934076-12934095 | MsG0280007274.01.T01:CDS | 35.0% | |
| TCAACTCTAATCCCATATTC+AGG | - | Chr2:12934247-12934266 | None:intergenic | 35.0% | |
| TGAAGACAATGAGTTGTAAC+AGG | - | Chr2:12934280-12934299 | None:intergenic | 35.0% | |
| TGGAACTTGTGGATTTGATA+CGG | + | Chr2:12934324-12934343 | MsG0280007274.01.T01:CDS | 35.0% | |
| TGTTCACAAAACATAGGCTT+TGG | - | Chr2:12934016-12934035 | None:intergenic | 35.0% | |
| ! | ATTGTACTAGTTTCGGGTAA+AGG | - | Chr2:12934466-12934485 | None:intergenic | 35.0% |
| ! | CTCAAAGATTCACTTGCTTT+TGG | - | Chr2:12934145-12934164 | None:intergenic | 35.0% |
| ! | GAGCTTTAATTTCTAGAGAG+CGG | + | Chr2:12934528-12934547 | MsG0280007274.01.T01:intron | 35.0% |
| ! | TTGTACTAGTTTCGGGTAAA+GGG | - | Chr2:12934465-12934484 | None:intergenic | 35.0% |
| !! | GTTGTTTTGTCTTGGAATTG+TGG | + | Chr2:12934933-12934952 | MsG0280007274.01.T01:intron | 35.0% |
| AATTGCACAGCACAATAGGA+AGG | + | Chr2:12934828-12934847 | MsG0280007274.01.T01:CDS | 40.0% | |
| CAATGTCAGGACACAATGAA+GGG | + | Chr2:12934298-12934317 | MsG0280007274.01.T01:CDS | 40.0% | |
| GTCTTGGAATTGTGGTTTCA+GGG | + | Chr2:12934941-12934960 | MsG0280007274.01.T01:intron | 40.0% | |
| GTTAGAGAAAAAGAACACCC+TGG | - | Chr2:12934097-12934116 | None:intergenic | 40.0% | |
| TCAATGTCAGGACACAATGA+AGG | + | Chr2:12934297-12934316 | MsG0280007274.01.T01:CDS | 40.0% | |
| TCTAAACTAAATGGTCGCGT+AGG | - | Chr2:12933914-12933933 | None:intergenic | 40.0% | |
| TGTCTTGGAATTGTGGTTTC+AGG | + | Chr2:12934940-12934959 | MsG0280007274.01.T01:intron | 40.0% | |
| TTCTGTTACCTGCGATTACA+TGG | - | Chr2:12934855-12934874 | None:intergenic | 40.0% | |
| TTGGAATTGGAGCTGGTATT+TGG | + | Chr2:12934995-12935014 | MsG0280007274.01.T01:CDS | 40.0% | |
| !!! | GGCTCATTGTTGTTTTGTCT+TGG | + | Chr2:12934925-12934944 | MsG0280007274.01.T01:intron | 40.0% |
| GGTACTTGATCATAAGGCTC+AGG | - | Chr2:12934226-12934245 | None:intergenic | 45.0% | |
| GTTTGAACTCCACATGTGAC+TGG | - | Chr2:12935048-12935067 | None:intergenic | 45.0% | |
| TGTTGGACCGTCTTGATATG+TGG | - | Chr2:12934591-12934610 | None:intergenic | 45.0% | |
| TTACAGCATTCTCAGCTGCA+GGG | + | Chr2:12934968-12934987 | MsG0280007274.01.T01:CDS | 45.0% | |
| AGGAAGGTCCATGTAATCGC+AGG | + | Chr2:12934844-12934863 | MsG0280007274.01.T01:CDS | 50.0% | |
| GTTACAGCATTCTCAGCTGC+AGG | + | Chr2:12934967-12934986 | MsG0280007274.01.T01:CDS | 50.0% | |
| TACAGCATTCTCAGCTGCAG+GGG | + | Chr2:12934969-12934988 | MsG0280007274.01.T01:CDS | 50.0% | |
| TGTCAGGACACAATGAAGGG+TGG | + | Chr2:12934301-12934320 | MsG0280007274.01.T01:CDS | 50.0% | |
| !! | ATGAAGGGTGGTGGAACTTG+TGG | + | Chr2:12934313-12934332 | MsG0280007274.01.T01:CDS | 50.0% |
| CAGGACACAATGAAGGGTGG+TGG | + | Chr2:12934304-12934323 | MsG0280007274.01.T01:CDS | 55.0% | |
| GCTACTGCACCAGTCACATG+TGG | + | Chr2:12935036-12935055 | MsG0280007274.01.T01:CDS | 55.0% | |
| GCTGCAGGGGCAATTGGAAT+TGG | + | Chr2:12934982-12935001 | MsG0280007274.01.T01:CDS | 55.0% | |
| GGGGCAATTGGAATTGGAGC+TGG | + | Chr2:12934988-12935007 | MsG0280007274.01.T01:CDS | 55.0% | |
| TTCTCAGCTGCAGGGGCAAT+TGG | + | Chr2:12934976-12934995 | MsG0280007274.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 12933878 | 12935089 | 12933878 | ID=MsG0280007274.01;Name=MsG0280007274.01 |
| Chr2 | mRNA | 12933878 | 12935089 | 12933878 | ID=MsG0280007274.01.T01;Parent=MsG0280007274.01;Name=MsG0280007274.01.T01;_AED=0.18;_eAED=0.18;_QI=0|0|0|1|1|1|3|0|232 |
| Chr2 | exon | 12933878 | 12934403 | 12933878 | ID=MsG0280007274.01.T01:exon:3802;Parent=MsG0280007274.01.T01 |
| Chr2 | exon | 12934821 | 12934865 | 12934821 | ID=MsG0280007274.01.T01:exon:3803;Parent=MsG0280007274.01.T01 |
| Chr2 | exon | 12934962 | 12935089 | 12934962 | ID=MsG0280007274.01.T01:exon:3804;Parent=MsG0280007274.01.T01 |
| Chr2 | CDS | 12933878 | 12934403 | 12933878 | ID=MsG0280007274.01.T01:cds;Parent=MsG0280007274.01.T01 |
| Chr2 | CDS | 12934821 | 12934865 | 12934821 | ID=MsG0280007274.01.T01:cds;Parent=MsG0280007274.01.T01 |
| Chr2 | CDS | 12934962 | 12935089 | 12934962 | ID=MsG0280007274.01.T01:cds;Parent=MsG0280007274.01.T01 |
| Gene Sequence |
| Protein sequence |
