AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007531.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007531.01.T01 MTR_2g027130 95.556 135 3 1 3 134 450 584 2.01e-83 255
MsG0280007531.01.T01 MTR_4g133450 45.238 126 61 1 6 131 197 314 1.87e-30 111
MsG0280007531.01.T01 MTR_4g134110 46.032 126 60 1 6 131 433 550 2.91e-30 114
MsG0280007531.01.T01 MTR_3g060660 43.396 106 60 0 13 118 465 570 1.97e-19 83.6
MsG0280007531.01.T01 MTR_3g060660 43.396 106 60 0 13 118 454 559 3.13e-19 82.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007531.01.T01 AT1G12150 49.107 112 55 2 7 116 432 543 1.19e-21 89.7
MsG0280007531.01.T01 AT1G12150 49.107 112 55 2 7 116 432 543 1.19e-21 89.7
MsG0280007531.01.T01 AT4G17210 40.541 111 66 0 3 113 413 523 5.46e-19 82.0

Find 16 sgRNAs with CRISPR-Local

Find 17 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATAAAAGCAGCAACAGATT+TGG 0.368777 2:+16692048 MsG0280007531.01.T01:CDS
TTCTAGCTCTGCAGCGAGAT+TGG 0.424207 2:-16692184 None:intergenic
TGGCGTCAAGAAGAACAAAA+AGG 0.451952 2:+16692133 MsG0280007531.01.T01:CDS
TCAGCAAAACTAGCAGTTGA+AGG 0.511221 2:+16692097 MsG0280007531.01.T01:CDS
AGAAACAACTGCAATGGCTC+AGG 0.523434 2:+16691955 MsG0280007531.01.T01:CDS
GATGAATTTGCTGCTCTAAG+TGG 0.529272 2:+16691896 MsG0280007531.01.T01:CDS
GGCTCTGAGGAATGCAGAGA+TGG 0.535386 2:+16692069 MsG0280007531.01.T01:CDS
AACAACTGCAATGGCTCAGG+TGG 0.549356 2:+16691958 MsG0280007531.01.T01:CDS
CTTACCACTGGAATCAGAAA+CGG 0.572360 2:-16691858 None:intergenic
TGTCAACACGATCTTACCAC+TGG 0.594761 2:-16691870 None:intergenic
AATGTGAAGATTTGATTGAG+AGG 0.600637 2:+16691930 MsG0280007531.01.T01:CDS
CAGCAACAGATTTGGCTCTG+AGG 0.613910 2:+16692056 MsG0280007531.01.T01:CDS
GAGGACAGAAACAACTGCAA+TGG 0.637220 2:+16691949 MsG0280007531.01.T01:CDS
GTCTCCGTTTCTGATTCCAG+TGG 0.639257 2:+16691854 MsG0280007531.01.T01:CDS
TTGAAGGTGAATTAAAGAAG+TGG 0.644395 2:+16692113 MsG0280007531.01.T01:CDS
AAGTTGTTGTCTGACGCACA+AGG 0.705883 2:+16691827 MsG0280007531.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AATAAAAGCAGCAACAGATT+TGG + Chr2:16692048-16692067 MsG0280007531.01.T01:CDS 30.0%
AATGTGAAGATTTGATTGAG+AGG + Chr2:16691930-16691949 MsG0280007531.01.T01:CDS 30.0%
ACAAAAAGGTTTGGATTTGT+CGG + Chr2:16692147-16692166 MsG0280007531.01.T01:CDS 30.0%
TCAAGAAGAACAAAAAGGTT+TGG + Chr2:16692138-16692157 MsG0280007531.01.T01:CDS 30.0%
TTGAAGGTGAATTAAAGAAG+TGG + Chr2:16692113-16692132 MsG0280007531.01.T01:CDS 30.0%
CTTACCACTGGAATCAGAAA+CGG - Chr2:16691861-16691880 None:intergenic 40.0%
TCAGCAAAACTAGCAGTTGA+AGG + Chr2:16692097-16692116 MsG0280007531.01.T01:CDS 40.0%
TGGCGTCAAGAAGAACAAAA+AGG + Chr2:16692133-16692152 MsG0280007531.01.T01:CDS 40.0%
!! GATGAATTTGCTGCTCTAAG+TGG + Chr2:16691896-16691915 MsG0280007531.01.T01:CDS 40.0%
AAGTTGTTGTCTGACGCACA+AGG + Chr2:16691827-16691846 MsG0280007531.01.T01:CDS 45.0%
AGAAACAACTGCAATGGCTC+AGG + Chr2:16691955-16691974 MsG0280007531.01.T01:CDS 45.0%
GAGGACAGAAACAACTGCAA+TGG + Chr2:16691949-16691968 MsG0280007531.01.T01:CDS 45.0%
TGTCAACACGATCTTACCAC+TGG - Chr2:16691873-16691892 None:intergenic 45.0%
AACAACTGCAATGGCTCAGG+TGG + Chr2:16691958-16691977 MsG0280007531.01.T01:CDS 50.0%
GTCTCCGTTTCTGATTCCAG+TGG + Chr2:16691854-16691873 MsG0280007531.01.T01:CDS 50.0%
!! CAGCAACAGATTTGGCTCTG+AGG + Chr2:16692056-16692075 MsG0280007531.01.T01:CDS 50.0%
!! GGCTCTGAGGAATGCAGAGA+TGG + Chr2:16692069-16692088 MsG0280007531.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr2 gene 16691800 16692204 16691800 ID=MsG0280007531.01;Name=MsG0280007531.01
Chr2 mRNA 16691800 16692204 16691800 ID=MsG0280007531.01.T01;Parent=MsG0280007531.01;Name=MsG0280007531.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|134
Chr2 exon 16691800 16692204 16691800 ID=MsG0280007531.01.T01:exon:1311;Parent=MsG0280007531.01.T01
Chr2 CDS 16691800 16692204 16691800 ID=MsG0280007531.01.T01:cds;Parent=MsG0280007531.01.T01
Gene Sequence

>MsG0280007531.01.T01

ATGAAAAAAAGAGCTGTTGAGGAGATGAAGTTGTTGTCTGACGCACAAGGTAGTGTCTCCGTTTCTGATTCCAGTGGTAAGATCGTGTTGACAGTTGATGAATTTGCTGCTCTAAGTGGAAAAATCAATGAATGTGAAGATTTGATTGAGAGGACAGAAACAACTGCAATGGCTCAGGTGGAAGCAATCAACACAAGAAGAAATGAAGTGAACAAAAAAGTCGAAGCTAATCTTCAAGCAATCGAAGAAATAAAAGCAGCAACAGATTTGGCTCTGAGGAATGCAGAGATGGCAGACTCAGCAAAACTAGCAGTTGAAGGTGAATTAAAGAAGTGGCGTCAAGAAGAACAAAAAGGTTTGGATTTGTCGGATAACTCTTCAACACCAATCTCGCTGCAGAGCTAG

Protein sequence

>MsG0280007531.01.T01

MKKRAVEEMKLLSDAQGSVSVSDSSGKIVLTVDEFAALSGKINECEDLIERTETTAMAQVEAINTRRNEVNKKVEANLQAIEEIKAATDLALRNAEMADSAKLAVEGELKKWRQEEQKGLDLSDNSSTPISLQS*