AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000914.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000914.01.T01 MTR_5g030935 96.923 65 2 0 11 75 21 85 3.50e-40 127
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000914.01.T01 AT2G22640 86.154 65 9 0 11 75 21 85 8.52e-36 116

Find 16 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTACTTGATTGCCAATTTAT+TGG 0.195626 1:-13149051 MsG0180000914.01.T01:three_prime_UTR
ATTTACTAATCCCAATAAAT+TGG 0.224559 1:+13149040 None:intergenic
TACTTGATTGCCAATTTATT+GGG 0.288967 1:-13149050 MsG0180000914.01.T01:three_prime_UTR
TGATGTATTGGAGCGTCGTT+TGG 0.343265 1:-13149291 MsG0180000914.01.T01:CDS
GAAGGATTTGAGTACCGAAT+TGG 0.379954 1:+13149448 None:intergenic
TGTATAATGTATAATCTAGA+TGG 0.379960 1:-13149155 MsG0180000914.01.T01:three_prime_UTR
TTCGATTTCCTTGTCCAATT+CGG 0.396293 1:-13149462 MsG0180000914.01.T01:intron
GATGTATTGGAGCGTCGTTT+GGG 0.412441 1:-13149290 MsG0180000914.01.T01:CDS
GAATGAGAAACTTGATGTAT+TGG 0.424872 1:-13149303 MsG0180000914.01.T01:CDS
ATCTAGATTATACATTATAC+AGG 0.460408 1:+13149157 None:intergenic
AGTTCAAGTTGGCAATGCTT+CGG 0.492873 1:-13149258 MsG0180000914.01.T01:CDS
GGGTTACTTGAAGTTCAAGT+TGG 0.567285 1:-13149269 MsG0180000914.01.T01:CDS
TTTGAGTACCGAATTGGACA+AGG 0.596328 1:+13149454 None:intergenic
ATGTATTGGAGCGTCGTTTG+GGG 0.620005 1:-13149289 MsG0180000914.01.T01:CDS
TATACATTATACAGGTGTGG+TGG 0.640579 1:+13149165 None:intergenic
GATTATACATTATACAGGTG+TGG 0.648256 1:+13149162 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAAAGTTAAAATTAAGA+AGG + Chr1:13149234-13149253 None:intergenic 15.0%
!!! AATCTTATATCTTTTTTTGA+TGG - Chr1:13149279-13149298 MsG0180000914.01.T01:CDS 15.0%
!!! ATCTTATATCTTTTTTTGAT+GGG - Chr1:13149280-13149299 MsG0180000914.01.T01:CDS 15.0%
!!! GTTTTTTTCAATTTAATAAC+AGG + Chr1:13149014-13149033 None:intergenic 15.0%
!! ATCTAGATTATACATTATAC+AGG + Chr1:13149507-13149526 None:intergenic 20.0%
!! ATTTACTAATCCCAATAAAT+TGG + Chr1:13149624-13149643 None:intergenic 20.0%
!! TGTATAATGTATAATCTAGA+TGG - Chr1:13149506-13149525 MsG0180000914.01.T01:CDS 20.0%
!!! TGTTATTTTTGTTATTTCAG+AGG - Chr1:13149307-13149326 MsG0180000914.01.T01:CDS 20.0%
! AAAAAAACATGTTGCTGAAT+TGG - Chr1:13149026-13149045 MsG0180000914.01.T01:three_prime_UTR 25.0%
! AAAAAACATGTTGCTGAATT+GGG - Chr1:13149027-13149046 MsG0180000914.01.T01:three_prime_UTR 25.0%
! TACTTGATTGCCAATTTATT+GGG - Chr1:13149611-13149630 MsG0180000914.01.T01:five_prime_UTR 25.0%
ATTTCAACTAACAACGCAAA+AGG + Chr1:13149262-13149281 None:intergenic 30.0%
GAATGAGAAACTTGATGTAT+TGG - Chr1:13149358-13149377 MsG0180000914.01.T01:intron 30.0%
GATTATACATTATACAGGTG+TGG + Chr1:13149502-13149521 None:intergenic 30.0%
GTACTTGATTGCCAATTTAT+TGG - Chr1:13149610-13149629 MsG0180000914.01.T01:five_prime_UTR 30.0%
TTTCAACTAACAACGCAAAA+GGG + Chr1:13149261-13149280 None:intergenic 30.0%
TATACATTATACAGGTGTGG+TGG + Chr1:13149499-13149518 None:intergenic 35.0%
TTCGATTTCCTTGTCCAATT+CGG - Chr1:13149199-13149218 MsG0180000914.01.T01:three_prime_UTR 35.0%
! CATCAAGTTGCAAAAAGAGA+AGG + Chr1:13149434-13149453 None:intergenic 35.0%
GGGTTACTTGAAGTTCAAGT+TGG - Chr1:13149392-13149411 MsG0180000914.01.T01:intron 40.0%
!! AGTTCAAGTTGGCAATGCTT+CGG - Chr1:13149403-13149422 MsG0180000914.01.T01:intron 40.0%
!! GAAGGATTTGAGTACCGAAT+TGG + Chr1:13149216-13149235 None:intergenic 40.0%
!! TTTGAGTACCGAATTGGACA+AGG + Chr1:13149210-13149229 None:intergenic 40.0%
!! ATGTATTGGAGCGTCGTTTG+GGG - Chr1:13149372-13149391 MsG0180000914.01.T01:intron 45.0%
!! GATGTATTGGAGCGTCGTTT+GGG - Chr1:13149371-13149390 MsG0180000914.01.T01:intron 45.0%
!! TGATGTATTGGAGCGTCGTT+TGG - Chr1:13149370-13149389 MsG0180000914.01.T01:intron 45.0%
Chromosome Type Strat End Strand Name
Chr1 gene 13149008 13149675 13149008 ID=MsG0180000914.01;Name=MsG0180000914.01
Chr1 mRNA 13149008 13149675 13149008 ID=MsG0180000914.01.T01;Parent=MsG0180000914.01;Name=MsG0180000914.01.T01;_AED=0.45;_eAED=0.45;_QI=110|1|1|1|1|1|2|224|75
Chr1 exon 13149008 13149356 13149008 ID=MsG0180000914.01.T01:exon:9240;Parent=MsG0180000914.01.T01
Chr1 exon 13149463 13149675 13149463 ID=MsG0180000914.01.T01:exon:9239;Parent=MsG0180000914.01.T01
Chr1 five_prime_UTR 13149566 13149675 13149566 ID=MsG0180000914.01.T01:five_prime_utr;Parent=MsG0180000914.01.T01
Chr1 CDS 13149463 13149565 13149463 ID=MsG0180000914.01.T01:cds;Parent=MsG0180000914.01.T01
Chr1 CDS 13149232 13149356 13149232 ID=MsG0180000914.01.T01:cds;Parent=MsG0180000914.01.T01
Chr1 three_prime_UTR 13149008 13149231 13149008 ID=MsG0180000914.01.T01:three_prime_utr;Parent=MsG0180000914.01.T01
Gene Sequence

>MsG0180000914.01.T01

ATGTTTGCATCTCTACGATACGATACGATGTATGAAAATCGTGAATTCATCTCTCACATTTCTCTCAATGTTCGTCGTCTCTTCGATTTCCTTGTCCAATTCGAGGCTACAACTAAGAGTAAATTAGCTTCATTGAATGAGAAACTTGATGTATTGGAGCGTCGTTTGGGGTTACTTGAAGTTCAAGTTGGCAATGCTTCGGCAAATCCTTCTCTTTTTGCAACTTGA

Protein sequence

>MsG0180000914.01.T01

MFASLRYDTMYENREFISHISLNVRRLFDFLVQFEATTKSKLASLNEKLDVLERRLGLLEVQVGNASANPSLFAT*