AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007545.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007545.01.T01 MTR_2g027540 95.582 249 6 2 11 254 1 249 2.02e-176 485
MsG0280007545.01.T01 MTR_2g027540 85.542 249 7 3 11 254 1 225 6.25e-143 400
MsG0280007545.01.T01 MTR_5g093740 51.759 199 95 1 46 244 8 205 3.80e-67 217
MsG0280007545.01.T01 MTR_5g093740 46.798 203 96 4 46 244 257 451 3.51e-50 172
MsG0280007545.01.T01 MTR_6g082260 48.500 200 101 2 46 244 4 202 1.80e-61 192
MsG0280007545.01.T01 MTR_6g082340 49.231 195 98 1 50 244 8 201 3.68e-61 192
MsG0280007545.01.T01 MTR_6g082310 47.980 198 101 2 50 246 8 204 5.51e-60 189
MsG0280007545.01.T01 MTR_3g061070 46.734 199 105 1 46 244 35 232 2.56e-57 189
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007545.01.T01 AT4G26485 48.529 204 101 3 44 244 4 206 6.84e-59 186
MsG0280007545.01.T01 AT5G56075 47.196 214 94 3 37 247 56 253 1.67e-56 181
MsG0280007545.01.T01 AT1G55790 43.111 225 114 2 22 246 8 218 1.11e-54 181
MsG0280007545.01.T01 AT5G25030 43.367 196 97 2 50 245 11 192 4.22e-48 158
MsG0280007545.01.T01 AT1G55803 48.800 125 64 0 32 156 3 127 3.22e-35 122
MsG0280007545.01.T01 AT5G56060 37.333 150 91 2 86 234 2 149 1.20e-30 112
MsG0280007545.01.T01 AT2G11045 33.121 157 69 4 90 245 14 135 1.76e-16 74.3
MsG0280007545.01.T01 AT1G55800 39.773 88 52 1 159 246 10 96 5.29e-14 69.7

Find 66 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTTTAGATCTTCACTTTA+TGG 0.136642 2:+16972873 MsG0280007545.01.T01:three_prime_UTR
CTTTGTTTAGCTAGGGCTTT+TGG 0.226805 2:+16970615 MsG0280007545.01.T01:CDS
AAAGTTGTTGAAGGGGTTTA+TGG 0.239937 2:+16972528 MsG0280007545.01.T01:CDS
AAGATAGGCTATAATTTGTT+AGG 0.280357 2:-16972802 None:intergenic
TAAAGTAACGATTGGTCAAT+AGG 0.290314 2:+16970354 MsG0280007545.01.T01:five_prime_UTR
TACTTAAACATTGTTGATAA+GGG 0.295882 2:+16972972 MsG0280007545.01.T01:three_prime_UTR
AAACACAAAATTCTCTTTGT+TGG 0.296100 2:+16970570 MsG0280007545.01.T01:CDS
AGCCACTTCCCTTGATTCTC+AGG 0.330415 2:+16970656 MsG0280007545.01.T01:CDS
CTTTCTCTCTTTGTTTAGCT+AGG 0.331146 2:+16970607 MsG0280007545.01.T01:CDS
CAGATTTATCTTTCCCTGTT+GGG 0.342684 2:+16972740 MsG0280007545.01.T01:CDS
ACAAGATCCCATTTGTTGTA+TGG 0.352391 2:-16972616 None:intergenic
CTACTTAAACATTGTTGATA+AGG 0.352461 2:+16972971 MsG0280007545.01.T01:three_prime_UTR
CATCATCGAACACAGCAAAA+TGG 0.353869 2:+16970529 MsG0280007545.01.T01:CDS
AAAGGAAACATTACTGAATC+TGG 0.360922 2:-16970960 None:intergenic
AATCAAGGGAAGTGGCTATA+AGG 0.366752 2:-16970650 None:intergenic
TAAGTACTGGCTTCCCCAAC+AGG 0.374095 2:-16972754 None:intergenic
ATTTGTTAGGGAGAGGTGTT+TGG 0.378150 2:-16972789 None:intergenic
AGGGTGATCCATACAACAAA+TGG 0.390906 2:+16972608 MsG0280007545.01.T01:CDS
TCTTTATGATGTTGATGTTA+AGG 0.404489 2:+16970840 MsG0280007545.01.T01:CDS
CAAAATTCTCTTTGTTGGAG+AGG 0.419240 2:+16970575 MsG0280007545.01.T01:CDS
TCAGATTTATCTTTCCCTGT+TGG 0.434452 2:+16972739 MsG0280007545.01.T01:CDS
ATCTGGCAGTAACTATTCTC+AGG 0.435024 2:-16970943 None:intergenic
TTTCTCTCTTTGTTTAGCTA+GGG 0.444044 2:+16970608 MsG0280007545.01.T01:CDS
AAAGTAACGATTGGTCAATA+GGG 0.447566 2:+16970355 MsG0280007545.01.T01:five_prime_UTR
TTAAATAGAGATAAAACATA+TGG 0.452221 2:-16972901 None:intergenic
TTGCATTACCCTGAGAATCA+AGG 0.455310 2:-16970665 None:intergenic
GAGCTTGAACTTGTAAGTAC+TGG 0.474156 2:-16972767 None:intergenic
GGCTATAATTTGTTAGGGAG+AGG 0.484720 2:-16972796 None:intergenic
GGATAGTCATCTTTGAAGAA+TGG 0.508198 2:-16972682 None:intergenic
TATGACAACAAGAGAGCTCA+TGG 0.509129 2:+16972709 MsG0280007545.01.T01:CDS
AGATAGGCTATAATTTGTTA+GGG 0.513605 2:-16972801 None:intergenic
TTCTTCAAAGATGACTATCC+TGG 0.517775 2:+16972685 MsG0280007545.01.T01:CDS
AAAGAAGTGGGACTAGAAGT+TGG 0.519198 2:-16972827 None:intergenic
AAAATTCTCTTTGTTGGAGA+GGG 0.521483 2:+16970576 MsG0280007545.01.T01:CDS
AGATTTATCTTTCCCTGTTG+GGG 0.524030 2:+16972741 MsG0280007545.01.T01:CDS
ACTAGTAAAGAAAGAGGGAG+GGG 0.524780 2:+16972564 MsG0280007545.01.T01:CDS
AGTAACTATTCTCAGGGTAA+AGG 0.525453 2:-16970936 None:intergenic
ATTGAAAAGAAGTATAGTAA+TGG 0.531936 2:+16970775 MsG0280007545.01.T01:CDS
AAAGGCACTAGTAAAGAAAG+AGG 0.540011 2:+16972558 MsG0280007545.01.T01:CDS
TGCATTACCCTGAGAATCAA+GGG 0.540315 2:-16970664 None:intergenic
GGGTGATCCATACAACAAAT+GGG 0.540448 2:+16972609 MsG0280007545.01.T01:CDS
GCCACTTCCCTTGATTCTCA+GGG 0.542014 2:+16970657 MsG0280007545.01.T01:CDS
GCAAGGGAACTTGAAGAAAG+AGG 0.562096 2:+16970808 MsG0280007545.01.T01:CDS
ACTTAAACATTGTTGATAAG+GGG 0.562256 2:+16972973 MsG0280007545.01.T01:three_prime_UTR
ACCCTGAGAATCAAGGGAAG+TGG 0.567810 2:-16970658 None:intergenic
ATAAGCCAAAATAAAAGAAG+TGG 0.568651 2:-16972840 None:intergenic
CTTTGAAGAATGGAACAGCT+TGG 0.571652 2:-16972672 None:intergenic
GTAATGGAATGAGTAATGCA+AGG 0.572801 2:+16970791 MsG0280007545.01.T01:CDS
GACTAGAAGTTGGCAAAGAT+AGG 0.577531 2:-16972817 None:intergenic
CACTAGTAAAGAAAGAGGGA+GGG 0.592759 2:+16972563 MsG0280007545.01.T01:CDS
GGGGTTTATGGCAAATGCAA+AGG 0.596492 2:+16972540 MsG0280007545.01.T01:CDS
TAACGATTGGTCAATAGGGT+CGG 0.596504 2:+16970359 MsG0280007545.01.T01:five_prime_UTR
AAGGGGTTTGTAATGAATGA+TGG 0.597722 2:+16972990 MsG0280007545.01.T01:three_prime_UTR
GCTCTCTTGTTGTCATAACC+AGG 0.598483 2:-16972703 None:intergenic
AGTGTGAGAGAGAAATAGTG+AGG 0.599035 2:+16970282 MsG0280007545.01.T01:five_prime_UTR
ATTTCTTTCTCAAGACTCAG+AGG 0.603232 2:+16970875 MsG0280007545.01.T01:CDS
TAAGCCAAAATAAAAGAAGT+GGG 0.605418 2:-16972839 None:intergenic
TAATGGAATGAGTAATGCAA+GGG 0.623573 2:+16970792 MsG0280007545.01.T01:CDS
GCACTAGTAAAGAAAGAGGG+AGG 0.626548 2:+16972562 MsG0280007545.01.T01:CDS
GATTCATGTAACTCACAAAG+AGG 0.650804 2:+16972588 MsG0280007545.01.T01:CDS
GGGTAAAGGAATCCAACATG+AGG 0.659464 2:-16970922 None:intergenic
TCTGGCAGTAACTATTCTCA+GGG 0.659879 2:-16970942 None:intergenic
AAGGCACTAGTAAAGAAAGA+GGG 0.660972 2:+16972559 MsG0280007545.01.T01:CDS
ATTCATGTAACTCACAAAGA+GGG 0.661176 2:+16972589 MsG0280007545.01.T01:CDS
AAGTACTGGCTTCCCCAACA+GGG 0.678090 2:-16972753 None:intergenic
TCAGTGACAGTGTTAAGTTG+AGG 0.766236 2:-16970471 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAACAAAAATGAAAAA+TGG - Chr2:16970723-16970742 None:intergenic 10.0%
!!! ATAATAACATCTTAAAATTT+TGG - Chr2:16971119-16971138 None:intergenic 10.0%
!! AAAAATAGTAGTAAATGAAA+AGG - Chr2:16971009-16971028 None:intergenic 15.0%
!! ATATACTTAAATTGAGATTA+AGG - Chr2:16971688-16971707 None:intergenic 15.0%
!! TAGATAAAATGTTATTATCT+TGG + Chr2:16971779-16971798 MsG0280007545.01.T01:intron 15.0%
!! TTAAATAGAGATAAAACATA+TGG - Chr2:16972904-16972923 None:intergenic 15.0%
!!! AAGATAATAACATTTTATCT+AGG - Chr2:16971780-16971799 None:intergenic 15.0%
!! AAAAAAGTAACAGCATAATT+TGG - Chr2:16972928-16972947 None:intergenic 20.0%
!! AAAAAGTAAAAAAGAGAGAA+AGG - Chr2:16970981-16971000 None:intergenic 20.0%
!! TAACATCTCAATTTAATGAT+GGG + Chr2:16971342-16971361 MsG0280007545.01.T01:intron 20.0%
!! TTAACATCTCAATTTAATGA+TGG + Chr2:16971341-16971360 MsG0280007545.01.T01:intron 20.0%
!!! ATTGAAAAGAAGTATAGTAA+TGG + Chr2:16970775-16970794 MsG0280007545.01.T01:CDS 20.0%
!!! CGGGATAATAATATATTTTA+TGG + Chr2:16972021-16972040 MsG0280007545.01.T01:intron 20.0%
!!! TAACATCTTAAAATTTTGGT+TGG - Chr2:16971115-16971134 None:intergenic 20.0%
!!! TACTTAAACATTGTTGATAA+GGG + Chr2:16972972-16972991 MsG0280007545.01.T01:three_prime_UTR 20.0%
!!! TACTTTTTTTTTTCTTTCTG+TGG + Chr2:16972939-16972958 MsG0280007545.01.T01:three_prime_UTR 20.0%
! AAAAATGGACATGCTAAAAA+AGG - Chr2:16970708-16970727 None:intergenic 25.0%
! AAACACAAAATTCTCTTTGT+TGG + Chr2:16970570-16970589 MsG0280007545.01.T01:CDS 25.0%
! AAATGTTATTATCTTGGACT+TGG + Chr2:16971785-16971804 MsG0280007545.01.T01:intron 25.0%
! AACATCTCAATTTAATGATG+GGG + Chr2:16971343-16971362 MsG0280007545.01.T01:intron 25.0%
! AATAGTCAGAATTTACATTG+GGG - Chr2:16971852-16971871 None:intergenic 25.0%
! ACAACCTTGATCTTAAAATT+TGG - Chr2:16971498-16971517 None:intergenic 25.0%
! AGGTCAAATATACTTAAATC+AGG - Chr2:16971668-16971687 None:intergenic 25.0%
! AGTAGCAATAGAAATTAAGT+AGG + Chr2:16971905-16971924 MsG0280007545.01.T01:intron 25.0%
! ATAAGCCAAAATAAAAGAAG+TGG - Chr2:16972843-16972862 None:intergenic 25.0%
! ATAGCCTATCCAAAATTTAT+TGG + Chr2:16971137-16971156 MsG0280007545.01.T01:intron 25.0%
! ATGTTTAGATCTTCACTTTA+TGG + Chr2:16972873-16972892 MsG0280007545.01.T01:three_prime_UTR 25.0%
! ATTTAAGGATGGAAATAGTA+AGG + Chr2:16971981-16972000 MsG0280007545.01.T01:intron 25.0%
! CAACCTTGATCTTAAAATTT+GGG - Chr2:16971497-16971516 None:intergenic 25.0%
! CAATAGTCAGAATTTACATT+GGG - Chr2:16971853-16971872 None:intergenic 25.0%
! CTAAAGTTACATTTGTAAGT+AGG + Chr2:16971043-16971062 MsG0280007545.01.T01:intron 25.0%
! CTACTTAAACATTGTTGATA+AGG + Chr2:16972971-16972990 MsG0280007545.01.T01:three_prime_UTR 25.0%
! CTACTTACAAATGTAACTTT+AGG - Chr2:16971045-16971064 None:intergenic 25.0%
! GTATGAAGAAACAAAAATAG+AGG - Chr2:16972065-16972084 None:intergenic 25.0%
! TAAGCCAAAATAAAAGAAGT+GGG - Chr2:16972842-16972861 None:intergenic 25.0%
! TAGCCTATCCAAAATTTATT+GGG + Chr2:16971138-16971157 MsG0280007545.01.T01:intron 25.0%
! TCAATAGTCAGAATTTACAT+TGG - Chr2:16971854-16971873 None:intergenic 25.0%
! TCTTTATGATGTTGATGTTA+AGG + Chr2:16970840-16970859 MsG0280007545.01.T01:CDS 25.0%
! TTAAGTCAACCTTAAAAAAC+TGG - Chr2:16971090-16971109 None:intergenic 25.0%
! TTCCATCCTTAAATTCTATT+AGG - Chr2:16971975-16971994 None:intergenic 25.0%
! TTCCTAATAGAATTTAAGGA+TGG + Chr2:16971970-16971989 MsG0280007545.01.T01:intron 25.0%
! TTGAACAAAAAGTTGTTGAA+GGG + Chr2:16972520-16972539 MsG0280007545.01.T01:CDS 25.0%
! TTTCTATTGCTACTCTAATT+TGG - Chr2:16971899-16971918 None:intergenic 25.0%
!! ACTTAAACATTGTTGATAAG+GGG + Chr2:16972973-16972992 MsG0280007545.01.T01:three_prime_UTR 25.0%
!! AGGTTTGACTAAATTTTACT+CGG + Chr2:16972001-16972020 MsG0280007545.01.T01:intron 25.0%
!! ATCTAGGATATATATAAACC+AGG - Chr2:16971764-16971783 None:intergenic 25.0%
!! GTTTATAATTTTCCTCATGT+TGG + Chr2:16970910-16970929 MsG0280007545.01.T01:CDS 25.0%
!! TCTAGGATATATATAAACCA+GGG - Chr2:16971763-16971782 None:intergenic 25.0%
!! TCTTTTCATAAAGTAACGAT+TGG + Chr2:16970346-16970365 MsG0280007545.01.T01:five_prime_UTR 25.0%
!!! AAGATAGGCTATAATTTGTT+AGG - Chr2:16972805-16972824 None:intergenic 25.0%
!!! AGATAGGCTATAATTTGTTA+GGG - Chr2:16972804-16972823 None:intergenic 25.0%
!!! ATAAACCAGGGTTTTAAAAA+AGG - Chr2:16971751-16971770 None:intergenic 25.0%
!!! TTACCCAAATTTTAAGATCA+AGG + Chr2:16971491-16971510 MsG0280007545.01.T01:intron 25.0%
AAAATTCTCTTTGTTGGAGA+GGG + Chr2:16970576-16970595 MsG0280007545.01.T01:CDS 30.0%
AAAGGAAACATTACTGAATC+TGG - Chr2:16970963-16970982 None:intergenic 30.0%
AAATGTATATGACCACAACT+TGG - Chr2:16971636-16971655 None:intergenic 30.0%
ATTCATGTAACTCACAAAGA+GGG + Chr2:16972589-16972608 MsG0280007545.01.T01:CDS 30.0%
CAAATATTGTCAACTAGTAG+CGG - Chr2:16972315-16972334 None:intergenic 30.0%
GATAGTGATGTAAACATACA+GGG + Chr2:16971433-16971452 MsG0280007545.01.T01:intron 30.0%
GCAATAGAAATTAAGTAGGT+AGG + Chr2:16971909-16971928 MsG0280007545.01.T01:intron 30.0%
GGTACATAAATACTGTATAG+CGG + Chr2:16972334-16972353 MsG0280007545.01.T01:intron 30.0%
GTAAGAAAAGCTGAAAAAAG+AGG + Chr2:16972637-16972656 MsG0280007545.01.T01:CDS 30.0%
GTAGTTGAATGACTCAAAAA+TGG - Chr2:16971820-16971839 None:intergenic 30.0%
GTGCTTCCTAATAGAATTTA+AGG + Chr2:16971966-16971985 MsG0280007545.01.T01:intron 30.0%
GTTGAACAAAAAGTTGTTGA+AGG + Chr2:16972519-16972538 MsG0280007545.01.T01:CDS 30.0%
TAATGGAATGAGTAATGCAA+GGG + Chr2:16970792-16970811 MsG0280007545.01.T01:CDS 30.0%
TGAACAAAAAGTTGTTGAAG+GGG + Chr2:16972521-16972540 MsG0280007545.01.T01:CDS 30.0%
TGTCTCAAGAATAGTTTGAA+AGG - Chr2:16972454-16972473 None:intergenic 30.0%
TTGACCTTGATGTTAAACAT+AGG + Chr2:16971708-16971727 MsG0280007545.01.T01:intron 30.0%
! AAAGTAACGATTGGTCAATA+GGG + Chr2:16970355-16970374 MsG0280007545.01.T01:five_prime_UTR 30.0%
! CCTTAAAAAACTGGCTTATA+AGG - Chr2:16971081-16971100 None:intergenic 30.0%
! CTCTCAATAAATAGCCATTT+TGG - Chr2:16971460-16971479 None:intergenic 30.0%
! GGTTTGACTAAATTTTACTC+GGG + Chr2:16972002-16972021 MsG0280007545.01.T01:intron 30.0%
! TAAAGTAACGATTGGTCAAT+AGG + Chr2:16970354-16970373 MsG0280007545.01.T01:five_prime_UTR 30.0%
! TGATAGTGATGTAAACATAC+AGG + Chr2:16971432-16971451 MsG0280007545.01.T01:intron 30.0%
! TTGACGTCTCAATTTAACTT+GGG - Chr2:16971256-16971275 None:intergenic 30.0%
! TTGCCCAATAAATTTTGGAT+AGG - Chr2:16971144-16971163 None:intergenic 30.0%
! TTTCTCTCTTTGTTTAGCTA+GGG + Chr2:16970608-16970627 MsG0280007545.01.T01:CDS 30.0%
!! ACTCTAATTTGGCTTGATTT+AGG - Chr2:16971888-16971907 None:intergenic 30.0%
!! TAGTCCCACTTCTTTTATTT+TGG + Chr2:16972835-16972854 MsG0280007545.01.T01:three_prime_UTR 30.0%
!! TGATGATTTTTCAGCTGATT+TGG - Chr2:16971320-16971339 None:intergenic 30.0%
!!! CCTTATAAGCCAGTTTTTTA+AGG + Chr2:16971078-16971097 MsG0280007545.01.T01:intron 30.0%
!!! TGATCATGTTGTTTTTGTGT+TGG - Chr2:16970450-16970469 None:intergenic 30.0%
AAAGTTGCACAACAACTTCA+TGG - Chr2:16972161-16972180 None:intergenic 35.0%
AAAGTTGTTGAAGGGGTTTA+TGG + Chr2:16972528-16972547 MsG0280007545.01.T01:CDS 35.0%
AAGGGGTTTGTAATGAATGA+TGG + Chr2:16972990-16973009 MsG0280007545.01.T01:three_prime_UTR 35.0%
AGATTTATCTTTCCCTGTTG+GGG + Chr2:16972741-16972760 MsG0280007545.01.T01:CDS 35.0%
AGTAACTATTCTCAGGGTAA+AGG - Chr2:16970939-16970958 None:intergenic 35.0%
ATGCACCAAACAAGAATACA+TGG - Chr2:16972396-16972415 None:intergenic 35.0%
ATTTCTTTCTCAAGACTCAG+AGG + Chr2:16970875-16970894 MsG0280007545.01.T01:CDS 35.0%
CAAAATTCTCTTTGTTGGAG+AGG + Chr2:16970575-16970594 MsG0280007545.01.T01:CDS 35.0%
CAGATTTATCTTTCCCTGTT+GGG + Chr2:16972740-16972759 MsG0280007545.01.T01:CDS 35.0%
CATCAAAAACCTTGATGTCA+TGG + Chr2:16971609-16971628 MsG0280007545.01.T01:intron 35.0%
CCATCAATGTCGTTAATTGT+GGG + Chr2:16972223-16972242 MsG0280007545.01.T01:intron 35.0%
CGCTACTAGTTGACAATATT+TGG + Chr2:16972313-16972332 MsG0280007545.01.T01:intron 35.0%
CTGATCCATGTATTCTTGTT+TGG + Chr2:16972388-16972407 MsG0280007545.01.T01:intron 35.0%
GATTCATGTAACTCACAAAG+AGG + Chr2:16972588-16972607 MsG0280007545.01.T01:CDS 35.0%
GTTGACGTCTCAATTTAACT+TGG - Chr2:16971257-16971276 None:intergenic 35.0%
TAAACATACAGGGACCAAAA+TGG + Chr2:16971443-16971462 MsG0280007545.01.T01:intron 35.0%
TATTCTTGAGACACAGTGTA+TGG + Chr2:16972461-16972480 MsG0280007545.01.T01:intron 35.0%
TCAGATTTATCTTTCCCTGT+TGG + Chr2:16972739-16972758 MsG0280007545.01.T01:CDS 35.0%
TCCATCAATGTCGTTAATTG+TGG + Chr2:16972222-16972241 MsG0280007545.01.T01:intron 35.0%
TCGTGGTTTCCTTTCAATAA+GGG + Chr2:16972111-16972130 MsG0280007545.01.T01:intron 35.0%
TCTGCCTATGTTTAACATCA+AGG - Chr2:16971715-16971734 None:intergenic 35.0%
TGGCTTGATTTAGGAAATAG+AGG - Chr2:16971879-16971898 None:intergenic 35.0%
TTATGACTACTATGACATCG+AGG - Chr2:16971194-16971213 None:intergenic 35.0%
TTCTTCAAAGATGACTATCC+TGG + Chr2:16972685-16972704 MsG0280007545.01.T01:CDS 35.0%
! AACTTTTTGTTCAACCTGCT+TGG - Chr2:16972514-16972533 None:intergenic 35.0%
! AAGGCACTAGTAAAGAAAGA+GGG + Chr2:16972559-16972578 MsG0280007545.01.T01:CDS 35.0%
! CTTTCTCTCTTTGTTTAGCT+AGG + Chr2:16970607-16970626 MsG0280007545.01.T01:CDS 35.0%
! GCTCTTTGCCCAATAAATTT+TGG - Chr2:16971149-16971168 None:intergenic 35.0%
! GGATAGTCATCTTTGAAGAA+TGG - Chr2:16972685-16972704 None:intergenic 35.0%
! GTAATGGAATGAGTAATGCA+AGG + Chr2:16970791-16970810 MsG0280007545.01.T01:CDS 35.0%
! TATTGGGCAAAGAGCAATTT+TGG + Chr2:16971154-16971173 MsG0280007545.01.T01:intron 35.0%
!! AAAGGCACTAGTAAAGAAAG+AGG + Chr2:16972558-16972577 MsG0280007545.01.T01:CDS 35.0%
!! ACAAGATCCCATTTGTTGTA+TGG - Chr2:16972619-16972638 None:intergenic 35.0%
!! GGATTTGCTTTCATTTTCGT+CGG - Chr2:16971279-16971298 None:intergenic 35.0%
!! TATTCTTGTTTGGTGCATTG+TGG + Chr2:16972398-16972417 MsG0280007545.01.T01:intron 35.0%
AAAGAAGTGGGACTAGAAGT+TGG - Chr2:16972830-16972849 None:intergenic 40.0%
AATCAAGGGAAGTGGCTATA+AGG - Chr2:16970653-16970672 None:intergenic 40.0%
ACTAGTAAAGAAAGAGGGAG+GGG + Chr2:16972564-16972583 MsG0280007545.01.T01:CDS 40.0%
AGGGTGATCCATACAACAAA+TGG + Chr2:16972608-16972627 MsG0280007545.01.T01:CDS 40.0%
AGTGTGAGAGAGAAATAGTG+AGG + Chr2:16970282-16970301 MsG0280007545.01.T01:five_prime_UTR 40.0%
ATCTGGCAGTAACTATTCTC+AGG - Chr2:16970946-16970965 None:intergenic 40.0%
ATGGGGAAGACTATGACTAA+CGG + Chr2:16971360-16971379 MsG0280007545.01.T01:intron 40.0%
ATGTCGTTAATTGTGGGTAG+CGG + Chr2:16972229-16972248 MsG0280007545.01.T01:intron 40.0%
CACAAACCTCTGTAATACCA+TGG - Chr2:16971548-16971567 None:intergenic 40.0%
CACAGTTACATTGTGATCCA+TGG + Chr2:16971528-16971547 MsG0280007545.01.T01:intron 40.0%
CACTAGTAAAGAAAGAGGGA+GGG + Chr2:16972563-16972582 MsG0280007545.01.T01:CDS 40.0%
CATCATCGAACACAGCAAAA+TGG + Chr2:16970529-16970548 MsG0280007545.01.T01:CDS 40.0%
CATGGTATTACAGAGGTTTG+TGG + Chr2:16971546-16971565 MsG0280007545.01.T01:intron 40.0%
CCCACAATTAACGACATTGA+TGG - Chr2:16972226-16972245 None:intergenic 40.0%
CGTGGTTTCCTTTCAATAAG+GGG + Chr2:16972112-16972131 MsG0280007545.01.T01:intron 40.0%
CTCGTGGTTTCCTTTCAATA+AGG + Chr2:16972110-16972129 MsG0280007545.01.T01:intron 40.0%
GACTAGAAGTTGGCAAAGAT+AGG - Chr2:16972820-16972839 None:intergenic 40.0%
GAGCTTGAACTTGTAAGTAC+TGG - Chr2:16972770-16972789 None:intergenic 40.0%
GGGTGATCCATACAACAAAT+GGG + Chr2:16972609-16972628 MsG0280007545.01.T01:CDS 40.0%
TAACAGACTGCAAAGTGAGT+GGG - Chr2:16971220-16971239 None:intergenic 40.0%
TATGACAACAAGAGAGCTCA+TGG + Chr2:16972709-16972728 MsG0280007545.01.T01:CDS 40.0%
TCAACTAGTAGCGGCTATAA+TGG - Chr2:16972306-16972325 None:intergenic 40.0%
TCCAAGAACCCCTTATTGAA+AGG - Chr2:16972123-16972142 None:intergenic 40.0%
TCCTTTCAATAAGGGGTTCT+TGG + Chr2:16972119-16972138 MsG0280007545.01.T01:intron 40.0%
TCTGGCAGTAACTATTCTCA+GGG - Chr2:16970945-16970964 None:intergenic 40.0%
TGAATTTGTGACAACCAAGC+AGG + Chr2:16972497-16972516 MsG0280007545.01.T01:intron 40.0%
TGCATTACCCTGAGAATCAA+GGG - Chr2:16970667-16970686 None:intergenic 40.0%
TGTGATCCATGGTATTACAG+AGG + Chr2:16971539-16971558 MsG0280007545.01.T01:intron 40.0%
TTGCATTACCCTGAGAATCA+AGG - Chr2:16970668-16970687 None:intergenic 40.0%
! ATGACTAACGGATGCAGATT+GGG + Chr2:16971372-16971391 MsG0280007545.01.T01:intron 40.0%
! CTTTGAAGAATGGAACAGCT+TGG - Chr2:16972675-16972694 None:intergenic 40.0%
! TAACGATTGGTCAATAGGGT+CGG + Chr2:16970359-16970378 MsG0280007545.01.T01:five_prime_UTR 40.0%
! TATGACTAACGGATGCAGAT+TGG + Chr2:16971371-16971390 MsG0280007545.01.T01:intron 40.0%
! TCAGTGACAGTGTTAAGTTG+AGG - Chr2:16970474-16970493 None:intergenic 40.0%
!! ATTTGTTAGGGAGAGGTGTT+TGG - Chr2:16972792-16972811 None:intergenic 40.0%
!! CTTTGTTTAGCTAGGGCTTT+TGG + Chr2:16970615-16970634 MsG0280007545.01.T01:CDS 40.0%
!! GGCTATAATTTGTTAGGGAG+AGG - Chr2:16972799-16972818 None:intergenic 40.0%
!! GGGGGGTTATTTTGTGTTTT+TGG + Chr2:16971392-16971411 MsG0280007545.01.T01:intron 40.0%
!!! GCAGACCTTTTTTAAAACCC+TGG + Chr2:16971743-16971762 MsG0280007545.01.T01:intron 40.0%
!!! TTTTCAGCTGATTTGGCAAG+TGG - Chr2:16971313-16971332 None:intergenic 40.0%
AACAGACTGCAAAGTGAGTG+GGG - Chr2:16971219-16971238 None:intergenic 45.0%
CAACTTGGACCATGACATCA+AGG - Chr2:16971621-16971640 None:intergenic 45.0%
CAATAAGGGGTTCTTGGAAC+TGG + Chr2:16972125-16972144 MsG0280007545.01.T01:intron 45.0%
CTAACAGACTGCAAAGTGAG+TGG - Chr2:16971221-16971240 None:intergenic 45.0%
GCAAGGGAACTTGAAGAAAG+AGG + Chr2:16970808-16970827 MsG0280007545.01.T01:CDS 45.0%
GCACTAGTAAAGAAAGAGGG+AGG + Chr2:16972562-16972581 MsG0280007545.01.T01:CDS 45.0%
GGGGTTTATGGCAAATGCAA+AGG + Chr2:16972540-16972559 MsG0280007545.01.T01:CDS 45.0%
GGGTAAAGGAATCCAACATG+AGG - Chr2:16970925-16970944 None:intergenic 45.0%
TGATGTCATGGTCCAAGTTG+TGG + Chr2:16971621-16971640 MsG0280007545.01.T01:intron 45.0%
! GCTCTCTTGTTGTCATAACC+AGG - Chr2:16972706-16972725 None:intergenic 45.0%
! TGACTAACGGATGCAGATTG+GGG + Chr2:16971373-16971392 MsG0280007545.01.T01:intron 45.0%
!! GTTTGGTGCATTGTGGACTA+TGG + Chr2:16972405-16972424 MsG0280007545.01.T01:intron 45.0%
ACAGACTGCAAAGTGAGTGG+GGG - Chr2:16971218-16971237 None:intergenic 50.0%
ACCCTGAGAATCAAGGGAAG+TGG - Chr2:16970661-16970680 None:intergenic 50.0%
AGCCACTTCCCTTGATTCTC+AGG + Chr2:16970656-16970675 MsG0280007545.01.T01:CDS 50.0%
GACATCGAGGTGCACATTCA+AGG - Chr2:16971181-16971200 None:intergenic 50.0%
GCCACTTCCCTTGATTCTCA+GGG + Chr2:16970657-16970676 MsG0280007545.01.T01:CDS 50.0%
GGAAACCACGAGAGCTAACT+TGG - Chr2:16972102-16972121 None:intergenic 50.0%
! ACTAACGGATGCAGATTGGG+GGG + Chr2:16971375-16971394 MsG0280007545.01.T01:intron 50.0%
! ATCAGCCAAGTTAGCTCTCG+TGG + Chr2:16972094-16972113 MsG0280007545.01.T01:intron 50.0%
! GACTAACGGATGCAGATTGG+GGG + Chr2:16971374-16971393 MsG0280007545.01.T01:intron 50.0%
!! AAGTACTGGCTTCCCCAACA+GGG - Chr2:16972756-16972775 None:intergenic 50.0%
!! AGCTGATTTGGCAAGTGGAG+AGG - Chr2:16971308-16971327 None:intergenic 50.0%
!! TAAGTACTGGCTTCCCCAAC+AGG - Chr2:16972757-16972776 None:intergenic 50.0%
CAGACTGCAAAGTGAGTGGG+GGG - Chr2:16971217-16971236 None:intergenic 55.0%
TGGCAAGTGGAGAGGCAAGT+GGG - Chr2:16971300-16971319 None:intergenic 55.0%
TTGGCAAGTGGAGAGGCAAG+TGG - Chr2:16971301-16971320 None:intergenic 55.0%
!! GGGTAGCGGAGAATAGCAAG+CGG + Chr2:16972243-16972262 MsG0280007545.01.T01:intron 55.0%
ATGGCGCTGTAGCACTGCTG+CGG - Chr2:16972287-16972306 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 16970232 16973048 16970232 ID=MsG0280007545.01;Name=MsG0280007545.01
Chr2 mRNA 16970232 16973048 16970232 ID=MsG0280007545.01.T01;Parent=MsG0280007545.01;Name=MsG0280007545.01.T01;_AED=0.44;_eAED=0.44;_QI=179|1|1|1|1|1|3|232|254
Chr2 exon 16970232 16970678 16970232 ID=MsG0280007545.01.T01:exon:3640;Parent=MsG0280007545.01.T01
Chr2 exon 16970770 16970968 16970770 ID=MsG0280007545.01.T01:exon:3641;Parent=MsG0280007545.01.T01
Chr2 exon 16972519 16973048 16972519 ID=MsG0280007545.01.T01:exon:3642;Parent=MsG0280007545.01.T01
Chr2 five_prime_UTR 16970232 16970410 16970232 ID=MsG0280007545.01.T01:five_prime_utr;Parent=MsG0280007545.01.T01
Chr2 CDS 16970411 16970678 16970411 ID=MsG0280007545.01.T01:cds;Parent=MsG0280007545.01.T01
Chr2 CDS 16970770 16970968 16970770 ID=MsG0280007545.01.T01:cds;Parent=MsG0280007545.01.T01
Chr2 CDS 16972519 16972816 16972519 ID=MsG0280007545.01.T01:cds;Parent=MsG0280007545.01.T01
Chr2 three_prime_UTR 16972817 16973048 16972817 ID=MsG0280007545.01.T01:three_prime_utr;Parent=MsG0280007545.01.T01
Gene Sequence

>MsG0280007545.01.T01

ATGATTGAATATCAATTGATCAAAATCAAAATGAGTCCAACACAAAAACAACATGATCATCCTCAACTTAACACTGTCACTGACACTGATGATGAAGAACAAGAATCAGAAGAAGAATCATCATCGAACACAGCAAAATGGAAAAAACACTACTCATCAAAACACAAAATTCTCTTTGTTGGAGAGGGTGATTTTTCTTTCTCTCTTTGTTTAGCTAGGGCTTTTGGTTCTGCTCATAACCTTATAGCCACTTCCCTTGATTCTCAGGAGAAAATTGAAAAGAAGTATAGTAATGGAATGAGTAATGCAAGGGAACTTGAAGAAAGAGGTTGTATTGTTCTTTATGATGTTGATGTTAAGGTGATGAGTCAGCATTTCTTTCTCAAGACTCAGAGGTTTGATCGTGTTGTTTATAATTTTCCTCATGTTGGATTCCTTTACCCTGAGAATAGTTACTGCCAGATTCAGTTGAACAAAAAGTTGTTGAAGGGGTTTATGGCAAATGCAAAGGCACTAGTAAAGAAAGAGGGAGGGGAGATTCATGTAACTCACAAAGAGGGTGATCCATACAACAAATGGGATCTTGTAAGAAAAGCTGAAAAAAGAGGACTGTTCTTACACCAAGCTGTTCCATTCTTCAAAGATGACTATCCTGGTTATGACAACAAGAGAGCTCATGGAAAGTTATCAGATTTATCTTTCCCTGTTGGGGAAGCCAGTACTTACAAGTTCAAGCTCCAAACACCTCTCCCTAACAAATTATAG
Protein sequence

>MsG0280007545.01.T01

MIEYQLIKIKMSPTQKQHDHPQLNTVTDTDDEEQESEEESSSNTAKWKKHYSSKHKILFVGEGDFSFSLCLARAFGSAHNLIATSLDSQEKIEKKYSNGMSNARELEERGCIVLYDVDVKVMSQHFFLKTQRFDRVVYNFPHVGFLYPENSYCQIQLNKKLLKGFMANAKALVKKEGGEIHVTHKEGDPYNKWDLVRKAEKRGLFLHQAVPFFKDDYPGYDNKRAHGKLSDLSFPVGEASTYKFKLQTPLPNKL*