Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014869.01.T01 | XP_013460341.1 | 97.73 | 925 | 21 | 0 | 1 | 925 | 1 | 925 | 0 | 1873 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014869.01.T01 | A0A072UXV6 | 97.730 | 925 | 21 | 0 | 1 | 925 | 1 | 925 | 0.0 | 1873 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000085.01 | MsG0380014869.01 | 0.820711 | 5.936987e-53 | 4.733228e-50 |
| MsG0180003948.01 | MsG0380014869.01 | 0.800320 | 1.522907e-48 | 7.070100e-46 |
| MsG0280006311.01 | MsG0380014869.01 | 0.809496 | 1.839090e-50 | 1.081420e-47 |
| MsG0280007545.01 | MsG0380014869.01 | 0.804963 | 1.678561e-49 | 8.773108e-47 |
| MsG0280008280.01 | MsG0380014869.01 | 0.803910 | 2.781867e-49 | 1.415118e-46 |
| MsG0280008862.01 | MsG0380014869.01 | 0.825215 | 5.293389e-54 | 4.794529e-51 |
| MsG0280009514.01 | MsG0380014869.01 | 0.831982 | 1.225733e-55 | 1.351832e-52 |
| MsG0280010779.01 | MsG0380014869.01 | 0.813367 | 2.652820e-51 | 1.729123e-48 |
| MsG0280011399.01 | MsG0380014869.01 | 0.817031 | 4.071616e-52 | 2.932380e-49 |
| MsG0380013636.01 | MsG0380014869.01 | 0.816260 | 6.059874e-52 | 4.273334e-49 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014869.01.T01 | MTR_3g465430 | 97.730 | 925 | 21 | 0 | 1 | 925 | 1 | 925 | 0.0 | 1873 |
| MsG0380014869.01.T01 | MTR_4g014680 | 57.447 | 940 | 364 | 10 | 1 | 922 | 9 | 930 | 0.0 | 1079 |
| MsG0380014869.01.T01 | MTR_1g071400 | 47.535 | 852 | 421 | 6 | 74 | 920 | 51 | 881 | 0.0 | 786 |
| MsG0380014869.01.T01 | MTR_1g071400 | 46.556 | 784 | 393 | 6 | 74 | 852 | 51 | 813 | 0.0 | 704 |
| MsG0380014869.01.T01 | MTR_1g071400 | 32.298 | 161 | 109 | 0 | 750 | 910 | 382 | 542 | 3.87e-18 | 90.1 |
| MsG0380014869.01.T01 | MTR_7g069700 | 51.935 | 672 | 296 | 7 | 1 | 654 | 9 | 671 | 0.0 | 672 |
| MsG0380014869.01.T01 | MTR_7g069700 | 72.963 | 270 | 73 | 0 | 653 | 922 | 773 | 1042 | 3.29e-131 | 421 |
| MsG0380014869.01.T01 | MTR_7g069700 | 32.338 | 201 | 132 | 1 | 385 | 581 | 830 | 1030 | 1.94e-23 | 107 |
| MsG0380014869.01.T01 | MTR_7g069700 | 30.978 | 184 | 127 | 0 | 727 | 910 | 413 | 596 | 4.18e-20 | 97.1 |
| MsG0380014869.01.T01 | MTR_1g071400 | 42.962 | 682 | 363 | 6 | 74 | 750 | 51 | 711 | 0.0 | 553 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014869.01.T01 | AT3G51770 | 63.036 | 863 | 316 | 3 | 62 | 924 | 89 | 948 | 0.0 | 1150 |
| MsG0380014869.01.T01 | AT3G51770 | 63.036 | 863 | 316 | 3 | 62 | 924 | 97 | 956 | 0.0 | 1150 |
| MsG0380014869.01.T01 | AT5G58550 | 56.587 | 926 | 378 | 7 | 1 | 919 | 1 | 909 | 0.0 | 1033 |
| MsG0380014869.01.T01 | AT4G02680 | 48.531 | 851 | 416 | 5 | 74 | 920 | 51 | 883 | 0.0 | 783 |
Find 174 sgRNAs with CRISPR-Local
Find 220 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACCATTCCACCTCAATGC+AGG | 0.545159 | 3:+61719503 | MsG0380014869.01.T01:CDS |
| AAACTCAAGAAGCTATACAT+TGG | 0.574044 | 3:+61720744 | MsG0380014869.01.T01:three_prime_UTR |
| AAAGTCTCTACAAGGTTGAT+TGG | 0.404114 | 3:-61717426 | None:intergenic |
| AAATATGAACCTTTGAGAAA+AGG | 0.411072 | 3:-61717074 | None:intergenic |
| AAATGATTTCTGAATTTCAA+TGG | 0.382753 | 3:-61719601 | None:intergenic |
| AACAAGATTTGCAAATTCTG+AGG | 0.534851 | 3:-61717346 | None:intergenic |
| AACCTGCTATGCTCAGAAAG+TGG | 0.607602 | 3:-61716745 | None:intergenic |
| AACTCTAGCAGGCTCTTTCC+AGG | 0.361101 | 3:-61719088 | None:intergenic |
| AAGAAATCAAGTGTGTTAGA+AGG | 0.403139 | 3:+61717859 | MsG0380014869.01.T01:CDS |
| AAGAGTGTGCTGCAGAGAAA+TGG | 0.531402 | 3:+61718405 | MsG0380014869.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACTCAAGAAGCTATACAT+TGG | + | Chr3:61720744-61720763 | MsG0380014869.01.T01:three_prime_UTR | 30.0% | |
| AACAAGATTTGCAAATTCTG+AGG | - | Chr3:61717349-61717368 | None:intergenic | 30.0% | |
| AAGAAATCAAGTGTGTTAGA+AGG | + | Chr3:61717859-61717878 | MsG0380014869.01.T01:CDS | 30.0% | |
| AATGATGAATGATCTGAACT+TGG | - | Chr3:61716946-61716965 | None:intergenic | 30.0% | |
| ATATTCGAGTATAAACCAGT+CGG | + | Chr3:61718611-61718630 | MsG0380014869.01.T01:CDS | 30.0% | |
| ATATTGTTCTGTGTCTGTTA+AGG | - | Chr3:61717284-61717303 | None:intergenic | 30.0% | |
| ATCTTCTTGTGATTCTCATT+TGG | + | Chr3:61718100-61718119 | MsG0380014869.01.T01:CDS | 30.0% | |
| ATGGAGTTAGATAAGTTTCT+AGG | + | Chr3:61718785-61718804 | MsG0380014869.01.T01:CDS | 30.0% | |
| ATGTAATGTAACTGTACAAG+AGG | - | Chr3:61720954-61720973 | None:intergenic | 30.0% | |
| ATTACATAACTTTACATGGG+AGG | + | Chr3:61718918-61718937 | MsG0380014869.01.T01:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 61716673 | 61721090 | 61716673 | ID=MsG0380014869.01;Name=MsG0380014869.01 |
| Chr3 | mRNA | 61716673 | 61721090 | 61716673 | ID=MsG0380014869.01.T01;Parent=MsG0380014869.01;Name=MsG0380014869.01.T01;_AED=0.11;_eAED=0.11;_QI=507|1|1|1|1|1|4|357|925 |
| Chr3 | exon | 61716673 | 61719134 | 61716673 | ID=MsG0380014869.01.T01:exon:6360;Parent=MsG0380014869.01.T01 |
| Chr3 | exon | 61719458 | 61719749 | 61719458 | ID=MsG0380014869.01.T01:exon:6361;Parent=MsG0380014869.01.T01 |
| Chr3 | exon | 61719831 | 61720140 | 61719831 | ID=MsG0380014869.01.T01:exon:6362;Parent=MsG0380014869.01.T01 |
| Chr3 | exon | 61720513 | 61721090 | 61720513 | ID=MsG0380014869.01.T01:exon:6363;Parent=MsG0380014869.01.T01 |
| Chr3 | five_prime_UTR | 61716673 | 61717179 | 61716673 | ID=MsG0380014869.01.T01:five_prime_utr;Parent=MsG0380014869.01.T01 |
| Chr3 | CDS | 61717180 | 61719134 | 61717180 | ID=MsG0380014869.01.T01:cds;Parent=MsG0380014869.01.T01 |
| Chr3 | CDS | 61719458 | 61719749 | 61719458 | ID=MsG0380014869.01.T01:cds;Parent=MsG0380014869.01.T01 |
| Chr3 | CDS | 61719831 | 61720140 | 61719831 | ID=MsG0380014869.01.T01:cds;Parent=MsG0380014869.01.T01 |
| Chr3 | CDS | 61720513 | 61720733 | 61720513 | ID=MsG0380014869.01.T01:cds;Parent=MsG0380014869.01.T01 |
| Chr3 | three_prime_UTR | 61720734 | 61721090 | 61720734 | ID=MsG0380014869.01.T01:three_prime_utr;Parent=MsG0380014869.01.T01 |
| Gene Sequence |
>MsG0380014869.01.T01 ATGCGTAGTTTGAAGATTGTTGAACGTTTTAAGAGTATTCAAGTTCATGCTCTTAGTTCTTCATCTGAAACTAATGGTGATAGCAAAACCAAACCTCACAACCTTAACAGACACAGAACAATATTGTCTTGGTCAAAATCAAAATTCAACAACAGTAACACAAAAACCTCAGAATTTGCAAATCTTGTTTCTCTTCAGTTACCTTCAACTGACACTATTGAACCATCTATAGAACCTTACCTTAAACCAATCAACCTTGTAGAGACTTTAGCAGAACTCTATCAACGGATTGAGTTTTGCTCTTCACAGTCTGAGAAAGTGACACTTTTTGTTGAACTTTTCTCTGTTTTGTATGGTCTTGGAGACCAGAAGCTACTCAGAAGATGTCTTAGAAATGCGCGGCAGAACGCGGAAGATGTGATGTCAAAGGTTGTGTTGTCTGCATGGTTGAGGTTTGAAAGAAGGGATGATGAACTTGTTGGTGTTTGTTCAATGGATTGTGGTGGTTATAATGTTCTTGAATGTCCAAAGATGAATTTGGAAAATGGGTTTAGTCCTTTTTCAATTAATGATCACTGCAAGTGTACTCAAGAAGAGAAAAAGCATGAAAATTTTGATAATGATGAGTGTGTTTGTTTGTCTGATGAGGAAAGTGATGTTTTGTTTTGTGTTGGGAATGAAGAAATCAAGTGTGTTAGAAGGAGAATTGCTTCACTTTCAGAACCATTGAATGCAATGCTTTGTGGTGGATTTCTAGAATCCAAAATGTGGAAAATTGATTTTTCAGGTAATGGATTATGTTCAGAAGGAATGAAAGCAGTTGAAGTTTACAGCAGAACAAAAAGATTGGATCTTTTTTGTCCCAATACTGTTTTAGAGCTTCTGTCATTTGCCAATAGGTTTTGTTGTGAGGAGATGAAATCTTCTTGTGATTCTCATTTGGCTTCAATTGTTGGAAATGTTGAAGATGCATTGATACTTATTGAGTATGGATTGGAAGAAAGTGCAAAACTTCTTGTAGTTTCTTGCTTGCAAATTTTTCTCAGGGAGCTTCCGAATTCGCTTCATAATTCAAAGGTGATAAATTTCTTATGTAGTTTTGAGTCAAAGGAAAAGTTGGAAAATTTAGGGTGTGCTACTTTCTTGTTGTACTATTTTCTCAGTCAGGTGGCAATGGAAGAAAGCATGGTATCGAAAACAACAGCGATGTTGTTGGAGAGAATGAAAGAGTGTGCTGCAGAGAAATGGCAGAAGAGTCTTGCATTGCATCAATTAGGGTGTGTGTTTCTTGAAAGAAGAGAGTATAAAGAAGCTCAACATTGCTTCGATGAAGCAGTTGAATTAGGCCATGTTTATTCCATAGCAGGTGTGGCTAGAACAAAGCATAAACAAGGTCAACCATATTCGGCTTATAAGTTAATTAGTTCACTCATATTCGAGTATAAACCAGTCGGTTGGATGTATCAAGAGAGAGCATTGTACAATATGGGAAGAGAAAAAGGTTTTGATTTGGATTTTGCAACTCAACTCGATCCTTCACTTTCATTTCCGTATAAATATAGAGCATTGGAGAAAGTCGAGGAGAAGCAAATAAAGGAAGGGATTATGGAGTTAGATAAGTTTCTAGGATTTAAGCTTTCACCTGATTGTTTAGAGTTGAGGGCTTGGTTGTATATTGCATTAGATGATTATGATAGTGCTATAAGAGATATTAGAGCTTTGTTAACCATAGAGGCTAATTACATAACTTTACATGGGAGGATCAAAGGTGAATGTTTGGTTCAAATTCTTCAAAGTAGAATTCAGAAGAAGAATCAGGCTGATTGTTGGATGCAATTATACCAACAATGGTCTTCCGTTGATGATGTTGGTTCTTTGGCTATTATACATCAGATGCTAGAGAATGAGCCTGGAAAGAGCCTGCTAGAGTTTCGTCTGTCTCTACTCCTCTTGAGATTAAACTGTCAAAAGGCTGCAATGCGCAGTTTGCGGCTGGCAAGAAACCATTCCACCTCAATGCAGGAGAGGCTAATTTATGAAGGATGGATTCTATACGATACTGGCTATCGTGACGAGGCTGTTACAAGGGCTGACAAATCCATTGAAATTCAGAAATCATTTGAAGCCTTTTTCCTAAAAGCATATGTGTTAGCAGATACAAATCTTGATCCTGAATCTTCTTCTTATGTTATTCAGCTTTTGAAAGAAGCACTTACATGTCCTTCAGACGGACTTCGCAAAGGACAAGCATTGAATAACATGGGGAGTATTTATGTGGATTGTGGTAAAATAGAACTTGCAAAGGAATGCTACAACAATGCACTTGCAATTAGGCACACAAGAGCTCATCAAGGTCTGGCGCGTGTTTATCATCAGAAAAATCAACGAAAGGCTGCATACGATGAGATGACAATGCTGATTGAGAAAGCGGAAAGTAATGCCTCGGCATATGAGAAACGATCAGAATACTGTGATCGTGAGATGGCGAAGGCTGATCTTGATGTTGCAACACACCTTGATCCTTTAAGGACATACCCTTATAGATACAGGGCAGCAGTGCTGATGGATGAACAAAAAGAAACAGAAGCAGTAGAAGAACTCACTAAGGCTATAAAATTCAAGCCTGATCTTCAAATGCTTCATCTGCGAGCCGCGTTTTACGAGTCAATGGGAGATCTATCTTCCGCTCTTAAAGATTGTCAAGCTGCTCTTTGTTTAGATCCAACCCATGCAGCCACACTTGATTTATGTCAAAGGATACAAAAGCTGAACTTTTGA |
| Protein sequence |
>MsG0380014869.01.T01 MRSLKIVERFKSIQVHALSSSSETNGDSKTKPHNLNRHRTILSWSKSKFNNSNTKTSEFANLVSLQLPSTDTIEPSIEPYLKPINLVETLAELYQRIEFCSSQSEKVTLFVELFSVLYGLGDQKLLRRCLRNARQNAEDVMSKVVLSAWLRFERRDDELVGVCSMDCGGYNVLECPKMNLENGFSPFSINDHCKCTQEEKKHENFDNDECVCLSDEESDVLFCVGNEEIKCVRRRIASLSEPLNAMLCGGFLESKMWKIDFSGNGLCSEGMKAVEVYSRTKRLDLFCPNTVLELLSFANRFCCEEMKSSCDSHLASIVGNVEDALILIEYGLEESAKLLVVSCLQIFLRELPNSLHNSKVINFLCSFESKEKLENLGCATFLLYYFLSQVAMEESMVSKTTAMLLERMKECAAEKWQKSLALHQLGCVFLERREYKEAQHCFDEAVELGHVYSIAGVARTKHKQGQPYSAYKLISSLIFEYKPVGWMYQERALYNMGREKGFDLDFATQLDPSLSFPYKYRALEKVEEKQIKEGIMELDKFLGFKLSPDCLELRAWLYIALDDYDSAIRDIRALLTIEANYITLHGRIKGECLVQILQSRIQKKNQADCWMQLYQQWSSVDDVGSLAIIHQMLENEPGKSLLEFRLSLLLLRLNCQKAAMRSLRLARNHSTSMQERLIYEGWILYDTGYRDEAVTRADKSIEIQKSFEAFFLKAYVLADTNLDPESSSYVIQLLKEALTCPSDGLRKGQALNNMGSIYVDCGKIELAKECYNNALAIRHTRAHQGLARVYHQKNQRKAAYDEMTMLIEKAESNASAYEKRSEYCDREMAKADLDVATHLDPLRTYPYRYRAAVLMDEQKETEAVEELTKAIKFKPDLQMLHLRAAFYESMGDLSSALKDCQAALCLDPTHAATLDLCQRIQKLNF* |
