Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008182.01.T01 | XP_039686404.1 | 67.29 | 107 | 8 | 1 | 1 | 107 | 103 | 182 | 2.19E-32 | 122 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008182.01.T01 | A0A396HQF5 | 67.290 | 107 | 8 | 1 | 1 | 107 | 118 | 197 | 2.03e-32 | 121 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048683.01 | MsG0280008182.01 | 0.806964 | 6.368471e-50 | 3.503663e-47 |
| MsG0080048856.01 | MsG0280008182.01 | 0.820703 | 5.962555e-53 | 4.752547e-50 |
| MsG0180000145.01 | MsG0280008182.01 | 0.806184 | 9.303254e-50 | 5.017165e-47 |
| MsG0180000470.01 | MsG0280008182.01 | 0.860845 | 1.554444e-63 | 4.308797e-60 |
| MsG0180000650.01 | MsG0280008182.01 | 0.806670 | 7.348750e-50 | 4.012244e-47 |
| MsG0180001141.01 | MsG0280008182.01 | 0.805004 | 1.645660e-49 | 8.609779e-47 |
| MsG0180001886.01 | MsG0280008182.01 | 0.802597 | 5.202970e-49 | 2.559023e-46 |
| MsG0180001892.01 | MsG0280008182.01 | 0.817648 | 2.957534e-52 | 2.166212e-49 |
| MsG0180002089.01 | MsG0280008182.01 | 0.849072 | 4.087538e-60 | 7.658403e-57 |
| MsG0180002492.01 | MsG0280008182.01 | 0.825033 | 5.842379e-54 | 5.264636e-51 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008182.01.T01 | MTR_2g438770 | 67.290 | 107 | 8 | 1 | 1 | 107 | 138 | 217 | 1.22e-35 | 121 |
| MsG0280008182.01.T01 | MTR_5g044730 | 67.290 | 107 | 8 | 1 | 1 | 107 | 146 | 225 | 1.38e-35 | 121 |
Find 41 sgRNAs with CRISPR-Local
Find 324 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACACCTGGTTTGGACAGA+TGG | 0.445920 | 2:+26814908 | None:intergenic |
| AACATGTCTACTAGTGGTGG+TGG | 0.535019 | 2:-26813469 | MsG0280008182.01.T01:three_prime_UTR |
| AAGAACACCGGTGAGGAGAG+AGG | 0.581305 | 2:-26811515 | MsG0280008182.01.T01:three_prime_UTR |
| AATAACACCTGGCATACAGA+TGG | 0.627198 | 2:-26816611 | MsG0280008182.01.T01:CDS |
| ACAACACAAGTTGAAACACC+TGG | 0.618669 | 2:+26814895 | None:intergenic |
| ACAAGTTGAAACACCTGGTT+TGG | 0.379086 | 2:+26814900 | None:intergenic |
| ACATTATTGAACCACTACAG+AGG | 0.686068 | 2:+26814820 | None:intergenic |
| ACCGGCGAAGGGAAGAACAC+CGG | 0.693537 | 2:-26811527 | MsG0280008182.01.T01:three_prime_UTR |
| ACCGGTGTTCTTCCCTTCGC+CGG | 0.466349 | 2:+26811526 | None:intergenic |
| ACTTCAACCATCTGTATGCC+AGG | 0.511077 | 2:+26816604 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATATCCTGTAAGTGACA+AGG | - | Chr2:26814364-26814383 | MsG0280008182.01.T01:intron | 30.0% | |
| AAACTACAACAGAGTAACTT+TGG | + | Chr2:26812169-26812188 | None:intergenic | 30.0% | |
| AAATATCCTGTAAGTGACAA+GGG | - | Chr2:26814365-26814384 | MsG0280008182.01.T01:intron | 30.0% | |
| AAGGAAGAAAAAGGGAAATT+AGG | + | Chr2:26813691-26813710 | None:intergenic | 30.0% | |
| AAGTACCTCATTTATGTAGT+TGG | - | Chr2:26815339-26815358 | MsG0280008182.01.T01:intron | 30.0% | |
| AATAAACCTCCAATGTTTAC+TGG | - | Chr2:26814756-26814775 | MsG0280008182.01.T01:intron | 30.0% | |
| ACATCTTAATAGAAGGGAAT+AGG | + | Chr2:26811800-26811819 | None:intergenic | 30.0% | |
| AGAGGACTTGAAATGAAAAA+TGG | + | Chr2:26815520-26815539 | None:intergenic | 30.0% | |
| AGATTTGAACAAGAAGATGA+AGG | + | Chr2:26816217-26816236 | None:intergenic | 30.0% | |
| AGTAAACATTGGAGGTTTAT+TGG | + | Chr2:26814757-26814776 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 26811486 | 26816668 | 26811486 | ID=MsG0280008182.01;Name=MsG0280008182.01 |
| Chr2 | mRNA | 26811486 | 26816668 | 26811486 | ID=MsG0280008182.01.T01;Parent=MsG0280008182.01;Name=MsG0280008182.01.T01;_AED=0.50;_eAED=0.78;_QI=0|0|0|1|0|0|4|249|107 |
| Chr2 | exon | 26816529 | 26816668 | 26816529 | ID=MsG0280008182.01.T01:exon:18778;Parent=MsG0280008182.01.T01 |
| Chr2 | exon | 26814835 | 26815017 | 26814835 | ID=MsG0280008182.01.T01:exon:18777;Parent=MsG0280008182.01.T01 |
| Chr2 | exon | 26813425 | 26813507 | 26813425 | ID=MsG0280008182.01.T01:exon:18776;Parent=MsG0280008182.01.T01 |
| Chr2 | exon | 26811486 | 26811652 | 26811486 | ID=MsG0280008182.01.T01:exon:18775;Parent=MsG0280008182.01.T01 |
| Chr2 | CDS | 26816529 | 26816668 | 26816529 | ID=MsG0280008182.01.T01:cds;Parent=MsG0280008182.01.T01 |
| Chr2 | CDS | 26814835 | 26815017 | 26814835 | ID=MsG0280008182.01.T01:cds;Parent=MsG0280008182.01.T01 |
| Chr2 | CDS | 26813507 | 26813507 | 26813507 | ID=MsG0280008182.01.T01:cds;Parent=MsG0280008182.01.T01 |
| Chr2 | three_prime_UTR | 26813425 | 26813506 | 26813425 | ID=MsG0280008182.01.T01:three_prime_utr;Parent=MsG0280008182.01.T01 |
| Chr2 | three_prime_UTR | 26811486 | 26811652 | 26811486 | ID=MsG0280008182.01.T01:three_prime_utr;Parent=MsG0280008182.01.T01 |
| Gene Sequence |
>MsG0280008182.01.T01 ATGCTGAAGCTGCATGCGACTGACGAGGTCGTGCCAATAACACCTGGCATACAGATGGTTGAAGTAACAACGCCTGCTTCTGATGAAAACTATGTTGATGTTGCTGCTGCTGTGCAATCCTTTTGTGAATACCTTGCCTCTGGCGAATCATGTTTTTGGTTTGTTCCTTCATATTGGATTTCATCACTGACATATGCAATTCTTCGGTGTCAATTTCTCAGGCTTCTCCATCTGTCCAAACCAGGTGTTTCAACTTGTGTTGTTCCTACGGCTGCAGCTGCTGCTGCATTTCTAATGTCTGATGGTGGTGGTACACCTCTGTAA |
| Protein sequence |
>MsG0280008182.01.T01 MLKLHATDEVVPITPGIQMVEVTTPASDENYVDVAAAVQSFCEYLASGESCFWFVPSYWISSLTYAILRCQFLRLLHLSKPGVSTCVVPTAAAAAAFLMSDGGGTPL* |
