AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180001886.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 12 sgRNAs with CRISPR-Local

Find 12 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCGACTTTGCTATCATTGA+CGG 0.418660 1:-28801139 None:intergenic
ATGAGAACAATGGATTTGTT+CGG 0.449405 1:+28801030 MsG0180001886.01.T01:CDS
ATCATTGACGGCAATACCTT+CGG 0.457365 1:-28801127 None:intergenic
CAACATCCATAGCCATGGTT+AGG 0.489502 1:-28801077 None:intergenic
AGATAACCTAACCATGGCTA+TGG 0.512476 1:+28801071 MsG0180001886.01.T01:CDS
TCAGCTCATCGAAATCGTCT+TGG 0.545230 1:-28801185 None:intergenic
TTGTTCGGAGAGCGAGATGG+AGG 0.578762 1:+28801045 MsG0180001886.01.T01:CDS
AATTTCGATATCTTATCCGA+AGG 0.608255 1:+28801111 MsG0180001886.01.T01:CDS
GATTTGTTCGGAGAGCGAGA+TGG 0.613269 1:+28801042 MsG0180001886.01.T01:CDS
GTCATCAACATCCATAGCCA+TGG 0.620045 1:-28801082 None:intergenic
TAGTCGAAGAATGAGAACAA+TGG 0.638863 1:+28801020 None:intergenic
AGGATCAGATAACCTAACCA+TGG 0.691590 1:+28801065 MsG0180001886.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAGTCTTATTTTTTTTCATA+TGG + Chr1:28801215-28801234 MsG0180001886.01.T01:CDS 20.0%
AATTTCGATATCTTATCCGA+AGG + Chr1:28801111-28801130 MsG0180001886.01.T01:CDS 30.0%
! ATGAGAACAATGGATTTGTT+CGG + Chr1:28801030-28801049 MsG0180001886.01.T01:CDS 30.0%
AGATAACCTAACCATGGCTA+TGG + Chr1:28801071-28801090 MsG0180001886.01.T01:CDS 40.0%
AGGATCAGATAACCTAACCA+TGG + Chr1:28801065-28801084 MsG0180001886.01.T01:CDS 40.0%
ATCATTGACGGCAATACCTT+CGG - Chr1:28801130-28801149 None:intergenic 40.0%
! AGCGACTTTGCTATCATTGA+CGG - Chr1:28801142-28801161 None:intergenic 40.0%
CAACATCCATAGCCATGGTT+AGG - Chr1:28801080-28801099 None:intergenic 45.0%
GTCATCAACATCCATAGCCA+TGG - Chr1:28801085-28801104 None:intergenic 45.0%
TCAGCTCATCGAAATCGTCT+TGG - Chr1:28801188-28801207 None:intergenic 45.0%
! GATTTGTTCGGAGAGCGAGA+TGG + Chr1:28801042-28801061 MsG0180001886.01.T01:CDS 50.0%
TTGTTCGGAGAGCGAGATGG+AGG + Chr1:28801045-28801064 MsG0180001886.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 28801030 28801242 28801030 ID=MsG0180001886.01;Name=MsG0180001886.01
Chr1 mRNA 28801030 28801242 28801030 ID=MsG0180001886.01.T01;Parent=MsG0180001886.01;Name=MsG0180001886.01.T01;_AED=0.34;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|70
Chr1 exon 28801030 28801242 28801030 ID=MsG0180001886.01.T01:exon:41993;Parent=MsG0180001886.01.T01
Chr1 CDS 28801030 28801242 28801030 ID=MsG0180001886.01.T01:cds;Parent=MsG0180001886.01.T01
Gene Sequence

>MsG0180001886.01.T01

ATGAGAACAATGGATTTGTTCGGAGAGCGAGATGGAGGATCAGATAACCTAACCATGGCTATGGATGTTGATGACGTCGACAATTTCGATATCTTATCCGAAGGTATTGCCGTCAATGATAGCAAAGTCGCTGATGCTGATTTCTTTCAATTCTTCCAAGACGATTTCGATGAGCTGATATCAAAGAGTCTTATTTTTTTTCATATGGATTAG

Protein sequence

>MsG0180001886.01.T01

MRTMDLFGERDGGSDNLTMAMDVDDVDNFDILSEGIAVNDSKVADADFFQFFQDDFDELISKSLIFFHMD*