AlfalfaGEDB | MsG0180002089.01

M. sativa cultivar ZhongmuNo.1 / MsG0180002089.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180002089.01.T01	AES64392.1	92.157	51	4	0	1	51	27	77	1.63E-24	97.1

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180002089.01.T01	G7ILN7	92.157	51	4	0	1	51	27	77	7.78e-25	97.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047869.01	MsG0180002089.01	0.837108	6.312756e-57	8.117548e-54
MsG0080048856.01	MsG0180002089.01	0.800284	1.549101e-48	7.185084e-46
MsG0080048874.01	MsG0180002089.01	0.809194	2.134119e-50	1.244924e-47
MsG0180000065.01	MsG0180002089.01	0.804998	1.650009e-49	8.631368e-47
MsG0180000145.01	MsG0180002089.01	0.804554	2.042719e-49	1.056378e-46
MsG0180000335.01	MsG0180002089.01	0.801221	9.974987e-49	4.737635e-46
MsG0180000470.01	MsG0180002089.01	0.864287	1.355607e-64	4.233797e-61
MsG0180000650.01	MsG0180002089.01	0.810282	1.245723e-50	7.477859e-48
MsG0180000828.01	MsG0180002089.01	0.819688	1.018234e-52	7.890392e-50
MsG0180001342.01	MsG0180002089.01	0.810334	1.213504e-50	7.294651e-48
MsG0180001410.01	MsG0180002089.01	0.830231	3.298699e-55	3.454929e-52
MsG0180001892.01	MsG0180002089.01	0.820002	8.632320e-53	6.748437e-50
MsG0180002089.01	MsG0180002195.01	0.801617	8.273835e-49	3.969043e-46
MsG0180002089.01	MsG0180002282.01	0.801552	8.531252e-49	4.085891e-46
MsG0180002089.01	MsG0180003438.01	0.805860	1.088332e-49	5.820951e-47
MsG0180002089.01	MsG0180003669.01	0.837596	4.734045e-57	6.179763e-54
MsG0180002089.01	MsG0180004264.01	0.848949	4.421927e-60	8.250985e-57
MsG0180002089.01	MsG0180004426.01	0.807548	4.790038e-50	2.675542e-47
MsG0180002089.01	MsG0180004765.01	0.817026	4.081904e-52	2.939394e-49
MsG0180002089.01	MsG0180005974.01	0.820466	6.756885e-53	5.350699e-50
MsG0180002089.01	MsG0280006324.01	0.828788	7.399115e-55	7.429381e-52
MsG0180002089.01	MsG0280006325.01	0.816635	4.996319e-52	3.559373e-49
MsG0180002089.01	MsG0280006456.01	0.831840	1.328936e-55	1.459583e-52
MsG0180002089.01	MsG0280006491.01	0.811729	6.053495e-51	3.776092e-48
MsG0180002089.01	MsG0280007064.01	0.819676	1.025014e-52	7.940107e-50
MsG0180002089.01	MsG0280007164.01	0.807678	4.495767e-50	2.519686e-47
MsG0180002089.01	MsG0280007547.01	0.826430	2.724292e-54	2.554423e-51
MsG0180002089.01	MsG0280007955.01	0.820389	7.039117e-53	5.562539e-50
MsG0180002089.01	MsG0280008182.01	0.849072	4.087538e-60	7.658403e-57
MsG0180002089.01	MsG0280008615.01	0.822657	2.106548e-53	1.774305e-50
MsG0180002089.01	MsG0280008733.01	0.810666	1.029293e-50	6.242478e-48
MsG0180002089.01	MsG0280009646.01	0.833655	4.707924e-56	5.456901e-53
MsG0180002089.01	MsG0280010443.01	0.853205	2.786830e-61	5.974882e-58
MsG0180002089.01	MsG0280010796.01	0.806363	8.530132e-50	4.620975e-47
MsG0180002089.01	MsG0280011032.01	0.834446	2.982542e-56	3.540528e-53
MsG0180002089.01	MsG0280011156.01	0.823732	1.182418e-53	1.026756e-50
MsG0180002089.01	MsG0380011609.01	0.815104	1.096697e-51	7.491819e-49
MsG0180002089.01	MsG0380011870.01	0.821465	3.978955e-53	3.239475e-50
MsG0180002089.01	MsG0380012702.01	0.808607	2.849498e-50	1.636415e-47
MsG0180002089.01	MsG0380013475.01	0.805832	1.103618e-49	5.898227e-47
MsG0180002089.01	MsG0380014251.01	0.831718	1.424128e-55	1.558556e-52
MsG0180002089.01	MsG0380014468.01	0.803155	3.990275e-49	1.990692e-46
MsG0180002089.01	MsG0380014573.01	0.822491	2.302605e-53	1.930218e-50
MsG0180002089.01	MsG0380014894.01	0.809846	1.546014e-50	9.175964e-48
MsG0180002089.01	MsG0380015566.01	0.826303	2.921249e-54	2.729083e-51
MsG0180002089.01	MsG0380015720.01	0.831872	1.304600e-55	1.434264e-52
MsG0180002089.01	MsG0380016004.01	0.800769	1.233602e-48	5.792270e-46
MsG0180002089.01	MsG0380016064.01	0.817339	3.470371e-52	2.520489e-49
MsG0180002089.01	MsG0380016160.01	-0.817560	3.095282e-52	2.261677e-49
MsG0180002089.01	MsG0380016475.01	0.825375	4.850270e-54	4.412920e-51
MsG0180002089.01	MsG0380016517.01	0.802613	5.163348e-49	2.540541e-46
MsG0180002089.01	MsG0380016910.01	0.834195	3.449672e-56	4.063573e-53
MsG0180002089.01	MsG0380017266.01	-0.801994	6.924451e-49	3.353687e-46
MsG0180002089.01	MsG0380017326.01	0.813041	3.128400e-51	2.021023e-48
MsG0180002089.01	MsG0380017461.01	0.808044	3.756968e-50	2.125937e-47
MsG0180002089.01	MsG0380017848.01	0.809908	1.499683e-50	8.915296e-48
MsG0180002089.01	MsG0480018163.01	0.820475	6.724579e-53	5.326307e-50
MsG0180002089.01	MsG0480018778.01	0.808794	2.599921e-50	1.500402e-47
MsG0180002089.01	MsG0480018883.01	0.812729	3.661828e-51	2.346215e-48
MsG0180002089.01	MsG0480018964.01	0.809951	1.467653e-50	8.734874e-48
MsG0180002089.01	MsG0480019337.01	0.818212	2.205353e-52	1.640489e-49
MsG0180002089.01	MsG0480019730.01	0.825161	5.451993e-54	4.930618e-51
MsG0180002089.01	MsG0480019873.01	0.803369	3.602730e-49	1.807176e-46
MsG0180002089.01	MsG0480021274.01	0.819380	1.197229e-52	9.197503e-50
MsG0180002089.01	MsG0480021536.01	0.814590	1.426007e-51	9.607904e-49
MsG0180002089.01	MsG0480021558.01	0.810786	9.691951e-51	5.896906e-48
MsG0180002089.01	MsG0480021853.01	0.825400	4.786250e-54	4.357804e-51
MsG0180002089.01	MsG0480021925.01	0.804142	2.489913e-49	1.274080e-46
MsG0180002089.01	MsG0480022213.01	-0.810465	1.137565e-50	6.861886e-48
MsG0180002089.01	MsG0480022259.01	0.831089	2.033330e-55	2.183836e-52
MsG0180002089.01	MsG0480022262.01	0.810649	1.037866e-50	6.291489e-48
MsG0180002089.01	MsG0480022615.01	-0.805924	1.055230e-49	5.652982e-47
MsG0180002089.01	MsG0480023015.01	0.809174	2.155677e-50	1.256804e-47
MsG0180002089.01	MsG0480023163.01	0.810289	1.241448e-50	7.453584e-48
MsG0180002089.01	MsG0480023654.01	0.808437	3.098000e-50	1.771414e-47
MsG0180002089.01	MsG0480024003.01	0.803141	4.016704e-49	2.003113e-46
MsG0180002089.01	MsG0480024005.01	0.817822	2.702178e-52	1.988710e-49
MsG0180002089.01	MsG0580024038.01	0.819339	1.223604e-52	9.389009e-50
MsG0180002089.01	MsG0580024552.01	0.807879	4.074920e-50	2.295740e-47
MsG0180002089.01	MsG0580024621.01	0.816835	4.504712e-52	3.226892e-49
MsG0180002089.01	MsG0580024905.01	0.808590	2.873419e-50	1.649460e-47
MsG0180002089.01	MsG0580025022.01	0.803298	3.726997e-49	1.866102e-46
MsG0180002089.01	MsG0580025135.01	0.809568	1.774333e-50	1.045406e-47
MsG0180002089.01	MsG0580025448.01	0.811343	7.342966e-51	4.533611e-48
MsG0180002089.01	MsG0580025638.01	0.819769	9.759811e-53	7.580651e-50
MsG0180002089.01	MsG0580025758.01	0.806646	7.435818e-50	4.057171e-47
MsG0180002089.01	MsG0580026002.01	0.807330	5.329147e-50	2.959969e-47
MsG0180002089.01	MsG0580026165.01	0.800177	1.628711e-48	7.533380e-46
MsG0180002089.01	MsG0580026342.01	0.823568	1.291345e-53	1.115967e-50
MsG0180002089.01	MsG0580027018.01	0.831520	1.593018e-55	1.732994e-52
MsG0180002089.01	MsG0580027650.01	0.800491	1.405834e-48	6.554762e-46
MsG0180002089.01	MsG0580027679.01	0.816776	4.644228e-52	3.321450e-49
MsG0180002089.01	MsG0580027687.01	0.836524	8.899362e-57	1.124313e-53
MsG0180002089.01	MsG0580027688.01	0.840806	6.965037e-58	1.003576e-54
MsG0180002089.01	MsG0580027745.01	0.803245	3.821296e-49	1.910798e-46
MsG0180002089.01	MsG0580027766.01	0.801652	8.139082e-49	3.907878e-46
MsG0180002089.01	MsG0580027833.01	0.801587	8.393289e-49	4.023240e-46
MsG0180002089.01	MsG0580027922.01	0.851381	9.209777e-61	1.860802e-57
MsG0180002089.01	MsG0580027947.01	0.803357	3.622618e-49	1.816605e-46
MsG0180002089.01	MsG0580028527.01	0.822219	2.663256e-53	2.215176e-50
MsG0180002089.01	MsG0580028634.01	0.804769	1.842803e-49	9.583358e-47
MsG0180002089.01	MsG0580028990.01	0.837709	4.430368e-57	5.803708e-54
MsG0180002089.01	MsG0580029204.01	0.824237	8.997396e-54	7.923834e-51
MsG0180002089.01	MsG0580029500.01	-0.811997	5.289928e-51	3.323524e-48
MsG0180002089.01	MsG0580029554.01	0.833319	5.709642e-56	6.552448e-53
MsG0180002089.01	MsG0680030316.01	0.807840	4.152677e-50	2.337260e-47
MsG0180002089.01	MsG0680030366.01	0.834481	2.923886e-56	3.474405e-53
MsG0180002089.01	MsG0680030667.01	0.841315	5.120933e-58	7.494803e-55
MsG0180002089.01	MsG0680030882.01	0.825600	4.291095e-54	3.929451e-51
MsG0180002089.01	MsG0680031210.01	0.808743	2.665992e-50	1.536473e-47
MsG0180002089.01	MsG0680031379.01	0.811760	5.957785e-51	3.719407e-48
MsG0180002089.01	MsG0680031505.01	0.807545	4.797017e-50	2.679278e-47
MsG0180002089.01	MsG0680032158.01	0.810614	1.056202e-50	6.396603e-48
MsG0180002089.01	MsG0680032801.01	0.848875	4.636276e-60	8.630455e-57
MsG0180002089.01	MsG0680032844.01	0.836366	9.762533e-57	1.227671e-53
MsG0180002089.01	MsG0680032996.01	0.803252	3.809728e-49	1.905325e-46
MsG0180002089.01	MsG0680033228.01	0.818181	2.241011e-52	1.665647e-49
MsG0180002089.01	MsG0680033379.01	0.843718	1.179076e-58	1.861057e-55
MsG0180002089.01	MsG0680033412.01	0.807045	6.123354e-50	3.375894e-47
MsG0180002089.01	MsG0680033792.01	0.832458	9.343512e-56	1.044958e-52
MsG0180002089.01	MsG0680034464.01	0.830719	2.506304e-55	2.662631e-52
MsG0180002089.01	MsG0680034466.01	0.809050	2.291648e-50	1.331616e-47
MsG0180002089.01	MsG0680034565.01	0.811689	6.175172e-51	3.847693e-48
MsG0180002089.01	MsG0680035444.01	0.841538	4.472858e-58	6.592787e-55
MsG0180002089.01	MsG0680035455.01	0.801605	8.320486e-49	3.990186e-46
MsG0180002089.01	MsG0680035475.01	0.810590	1.068919e-50	6.469414e-48
MsG0180002089.01	MsG0680035573.01	0.806021	1.006727e-49	5.406732e-47
MsG0180002089.01	MsG0680035692.01	0.829917	3.935598e-55	4.084794e-52
MsG0180002089.01	MsG0680035828.01	0.806264	8.952327e-50	4.837712e-47
MsG0180002089.01	MsG0780035948.01	0.823477	1.356352e-53	1.169131e-50
MsG0180002089.01	MsG0780036477.01	0.817240	3.654389e-52	2.646844e-49
MsG0180002089.01	MsG0780036498.01	0.829428	5.177098e-55	5.297104e-52
MsG0180002089.01	MsG0780037054.01	0.818887	1.550244e-52	1.174962e-49
MsG0180002089.01	MsG0780037457.01	0.826385	2.793547e-54	2.615958e-51
MsG0180002089.01	MsG0780038401.01	0.812634	3.841649e-51	2.455275e-48
MsG0180002089.01	MsG0780038827.01	0.802737	4.867377e-49	2.402594e-46
MsG0180002089.01	MsG0780039829.01	0.803754	2.997597e-49	1.518666e-46
MsG0180002089.01	MsG0780039867.01	-0.819298	1.249948e-52	9.580565e-50
MsG0180002089.01	MsG0780040057.01	0.813603	2.354508e-51	1.544522e-48
MsG0180002089.01	MsG0780040576.01	-0.819822	9.488727e-53	7.380821e-50
MsG0180002089.01	MsG0780040596.01	0.841143	5.679783e-58	8.269206e-55
MsG0180002089.01	MsG0780040704.01	0.823175	1.595362e-53	1.363512e-50
MsG0180002089.01	MsG0880041836.01	0.826194	3.100335e-54	2.887507e-51
MsG0180002089.01	MsG0880042161.01	0.818230	2.184324e-52	1.625615e-49
MsG0180002089.01	MsG0880042232.01	0.813811	2.119228e-51	1.398037e-48
MsG0180002089.01	MsG0880042294.01	0.806168	9.378887e-50	5.055808e-47
MsG0180002089.01	MsG0880042404.01	0.801010	1.101621e-48	5.204826e-46
MsG0180002089.01	MsG0880042454.01	0.823065	1.692942e-53	1.442476e-50
MsG0180002089.01	MsG0880042800.01	0.858405	8.426449e-63	2.148245e-59
MsG0180002089.01	MsG0880042886.01	0.822858	1.891617e-53	1.602329e-50
MsG0180002089.01	MsG0880042966.01	0.843115	1.708481e-58	2.645742e-55
MsG0180002089.01	MsG0880044323.01	0.824530	7.679748e-54	6.820663e-51
MsG0180002089.01	MsG0880044415.01	0.825695	4.075028e-54	3.741642e-51
MsG0180002089.01	MsG0880044416.01	0.821308	4.325343e-53	3.506274e-50
MsG0180002089.01	MsG0880044542.01	0.814269	1.679191e-51	1.121590e-48
MsG0180002089.01	MsG0880044554.01	0.814714	1.338620e-51	9.049242e-49
MsG0180002089.01	MsG0880044574.01	0.848830	4.772980e-60	8.871618e-57
MsG0180002089.01	MsG0880045362.01	0.804556	2.041205e-49	1.055640e-46
MsG0180002089.01	MsG0880045765.01	0.804340	2.264564e-49	1.164668e-46
MsG0180002089.01	MsG0880046457.01	0.802832	4.652632e-49	2.302230e-46
MsG0180002089.01	MsG0880046581.01	0.838442	2.868930e-57	3.843242e-54
MsG0180002089.01	MsG0880046620.01	0.800549	1.367903e-48	6.387247e-46
MsG0180002089.01	MsG0880046645.01	0.803293	3.735260e-49	1.870044e-46
MsG0180002089.01	MsG0880046803.01	0.810295	1.237273e-50	7.429795e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180002089.01.T01	MTR_2g021870	92.157	51	4	0	1	51	27	77	1.97e-28	97.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 10 sgRNAs with CRISPR-Local

Find 14 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATAATGAAGATAATGAAAA+TGG	0.293353	1:-32659363	MsG0180002089.01.T01:CDS
TCATTATCTTCATTATTGAT+TGG	0.384406	1:+32659369	None:intergenic
CTTCATTATTGATTGGACTA+GGG	0.456427	1:+32659376	None:intergenic
TCTTCATTATTGATTGGACT+AGG	0.459814	1:+32659375	None:intergenic
GATAATACAAGCTGCTAGAC+AGG	0.497499	1:-32659640	MsG0180002089.01.T01:intron
TTCATTATTGATTGGACTAG+GGG	0.510352	1:+32659377	None:intergenic
TTGGACTAGGGGAATTCAAA+GGG	0.520133	1:+32659388	None:intergenic
ATTGGACTAGGGGAATTCAA+AGG	0.524515	1:+32659387	None:intergenic
CTAGGGGAATTCAAAGGGTT+AGG	0.527124	1:+32659393	None:intergenic
GATAGAGTTAAGACTATGGA+GGG	0.632932	1:-32659723	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATAATGAAGATAATGAAAA+TGG	-	Chr1:32659659-32659678	MsG0180002089.01.T01:CDS	15.0%
!!	TCATTATCTTCATTATTGAT+TGG	+	Chr1:32659656-32659675	None:intergenic	20.0%
!!!	TTTTTGATTTTCTGTGATAT+AGG	-	Chr1:32659586-32659605	MsG0180002089.01.T01:intron	20.0%
!!!	AAATCAAGAGTATTGCTAAA+TGG	+	Chr1:32659545-32659564	None:intergenic	25.0%
!!!	TTCATTGATTTAAGAGAACA+TGG	-	Chr1:32659447-32659466	MsG0180002089.01.T01:intron	25.0%
!	CTTCATTATTGATTGGACTA+GGG	+	Chr1:32659649-32659668	None:intergenic	30.0%
!	TCTTCATTATTGATTGGACT+AGG	+	Chr1:32659650-32659669	None:intergenic	30.0%
!	TTCATTATTGATTGGACTAG+GGG	+	Chr1:32659648-32659667	None:intergenic	30.0%
!!	TTGATTCAAAGTCGATTTTC+TGG	-	Chr1:32659344-32659363	MsG0180002089.01.T01:CDS	30.0%
	GATAATACAAGCTGCTAGAC+AGG	-	Chr1:32659382-32659401	MsG0180002089.01.T01:CDS	40.0%
	GTTTGAGTCACACAATTCGT+TGG	-	Chr1:32659513-32659532	MsG0180002089.01.T01:intron	40.0%
!	ATTGGACTAGGGGAATTCAA+AGG	+	Chr1:32659638-32659657	None:intergenic	40.0%
!	TTGGACTAGGGGAATTCAAA+GGG	+	Chr1:32659637-32659656	None:intergenic	40.0%
	CTAGGGGAATTCAAAGGGTT+AGG	+	Chr1:32659632-32659651	None:intergenic	45.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	32659314	32659730	32659314	ID=MsG0180002089.01;Name=MsG0180002089.01
Chr1	mRNA	32659314	32659730	32659314	ID=MsG0180002089.01.T01;Parent=MsG0180002089.01;Name=MsG0180002089.01.T01;_AED=0.50;_eAED=0.61;_QI=0\|0\|0\|1\|1\|0.5\|2\|0\|70
Chr1	exon	32659641	32659730	32659641	ID=MsG0180002089.01.T01:exon:36603;Parent=MsG0180002089.01.T01
Chr1	exon	32659314	32659436	32659314	ID=MsG0180002089.01.T01:exon:36604;Parent=MsG0180002089.01.T01
Chr1	CDS	32659641	32659730	32659641	ID=MsG0180002089.01.T01:cds;Parent=MsG0180002089.01.T01
Chr1	CDS	32659314	32659436	32659314	ID=MsG0180002089.01.T01:cds;Parent=MsG0180002089.01.T01

Gene Sequence	>MsG0180002089.01.T01 ATGGAGGGCTTGTTAGCAAATGTCATGACTTTGATTCAAAGTCGATTTTCTGGAGATGATGTGAATGAGATAATACAAGCTGCTAGACAGGTTACTGATGCTTCTAGTGTTCCTAACCCTTTGAATTCCCCTAGTCCAATCAATAATGAAGATAATGAAAATGGTATTCAATTAAATATCACTGAAGTTGTTAATATTTTCTTTGTTTTTTAG
Protein sequence	>MsG0180002089.01.T01 MEGLLANVMTLIQSRFSGDDVNEIIQAARQVTDASSVPNPLNSPSPINNEDNENGIQLNITEVVNIFFVF*