AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280009565.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009565.01.T01 MTR_2g067080 98.131 107 2 0 13 119 275 381 1.46e-73 223
MsG0280009565.01.T02 MTR_2g067080 98.131 107 2 0 13 119 275 381 1.46e-73 223
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009565.01.T01 AT1G79230 75.472 106 26 0 13 118 176 281 9.70e-59 182
MsG0280009565.01.T01 AT1G79230 75.472 106 26 0 13 118 216 321 2.19e-58 183
MsG0280009565.01.T01 AT1G79230 75.472 106 26 0 13 118 273 378 3.55e-58 184
MsG0280009565.01.T01 AT1G16460 73.000 100 27 0 13 112 214 313 2.07e-51 165
MsG0280009565.01.T01 AT1G16460 73.000 100 27 0 13 112 214 313 2.07e-51 165
MsG0280009565.01.T01 AT1G16460 73.000 100 27 0 13 112 214 313 2.07e-51 165
MsG0280009565.01.T01 AT1G16460 73.000 100 27 0 13 112 238 337 2.73e-51 165
MsG0280009565.01.T01 AT1G16460 76.000 50 12 0 13 62 214 263 1.18e-21 87.0
MsG0280009565.01.T01 AT1G16460 76.000 50 12 0 13 62 238 287 1.81e-21 86.7
MsG0280009565.01.T02 AT1G79230 75.472 106 26 0 13 118 176 281 9.70e-59 182
MsG0280009565.01.T02 AT1G79230 75.472 106 26 0 13 118 216 321 2.19e-58 183
MsG0280009565.01.T02 AT1G79230 75.472 106 26 0 13 118 273 378 3.55e-58 184
MsG0280009565.01.T02 AT1G16460 73.000 100 27 0 13 112 214 313 2.07e-51 165
MsG0280009565.01.T02 AT1G16460 73.000 100 27 0 13 112 214 313 2.07e-51 165
MsG0280009565.01.T02 AT1G16460 73.000 100 27 0 13 112 214 313 2.07e-51 165
MsG0280009565.01.T02 AT1G16460 73.000 100 27 0 13 112 238 337 2.73e-51 165
MsG0280009565.01.T02 AT1G16460 76.000 50 12 0 13 62 214 263 1.18e-21 87.0
MsG0280009565.01.T02 AT1G16460 76.000 50 12 0 13 62 238 287 1.81e-21 86.7

Find 25 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGCTTTATATTATTGTTT+TGG 0.219662 2:-54413281 MsG0280009565.01.T01:three_prime_UTR
TGGATTAATTGTGATTAAAT+TGG 0.225379 2:-54413261 MsG0280009565.01.T01:three_prime_UTR
AAATTAAAATGAATGATAAA+AGG 0.237710 2:-54413146 MsG0280009565.01.T01:intron
CAAAGATGCATAGATAAATT+AGG 0.311697 2:+54413300 None:intergenic
TTAGGTGAGCTCAGAATGTT+TGG 0.338838 2:+54413318 None:intergenic
GTTTGTTCGGCGGTGTCAAC+AGG 0.383804 2:+54413395 None:intergenic
TCTTGGAAAGACCGATGTTC+CGG 0.407286 2:-54413462 MsG0280009565.01.T01:CDS
GCGGTGTCAACAGGTGTATC+AGG 0.430114 2:+54413404 None:intergenic
ACCGATGTTCCGGTTTATGA+TGG 0.443384 2:-54413452 MsG0280009565.01.T01:CDS
TTACAGGGTCTTCATCGTCT+TGG 0.449435 2:-54413479 MsG0280009565.01.T01:intron
TTCCGGTTTATGATGGATCA+TGG 0.453486 2:-54413445 MsG0280009565.01.T01:CDS
TTTACTATTAGTATTGTTGT+TGG 0.454393 2:+54413225 None:intergenic
GTCAAATAAAGTCAAAAGTA+AGG 0.456664 2:+54413066 None:intergenic
AAATAACGATAGGATTGTTA+AGG 0.462644 2:-54413182 MsG0280009565.01.T01:three_prime_UTR
GTGGCTCTATTGCGTTTGTT+CGG 0.465727 2:+54413382 None:intergenic
TAGGATTGTTAAGGAGGTTG+TGG 0.474801 2:-54413173 MsG0280009565.01.T01:intron
GTCCATGATCCATCATAAAC+CGG 0.478419 2:+54413443 None:intergenic
AAAAGTATCTGAATGGCAAA+AGG 0.491210 2:-54413357 MsG0280009565.01.T01:three_prime_UTR
ATGATGGATCATGGACTGAA+TGG 0.498892 2:-54413436 MsG0280009565.01.T01:CDS
TGATGGATCATGGACTGAAT+GGG 0.498922 2:-54413435 MsG0280009565.01.T01:CDS
TAACGATAGGATTGTTAAGG+AGG 0.519173 2:-54413179 MsG0280009565.01.T01:three_prime_UTR
GCTCTATTGCGTTTGTTCGG+CGG 0.575727 2:+54413385 None:intergenic
AAGTGTACTTGCATTGAAAG+TGG 0.575781 2:-54413110 MsG0280009565.01.T01:three_prime_UTR
TCCATCATAAACCGGAACAT+CGG 0.647529 2:+54413451 None:intergenic
GATGGATCATGGACTGAATG+GGG 0.715779 2:-54413434 MsG0280009565.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAAAATGAATGATAAA+AGG - Chr2:54414203-54414222 MsG0280009565.01.T01:intron 10.0%
!! TGTAAATACTTTGATATATT+GGG - Chr2:54413810-54413829 MsG0280009565.01.T01:intron 15.0%
!!! TTTGCTTTATATTATTGTTT+TGG - Chr2:54414068-54414087 MsG0280009565.01.T01:intron 15.0%
!!! TTTTCATTTAAAATGAGATA+TGG - Chr2:54414324-54414343 MsG0280009565.01.T01:CDS 15.0%
!! CTTAATAACAATAACGATAT+CGG + Chr2:54413205-54413224 None:intergenic 20.0%
!! GTGTAAATACTTTGATATAT+TGG - Chr2:54413809-54413828 MsG0280009565.01.T01:intron 20.0%
!! TGGATTAATTGTGATTAAAT+TGG - Chr2:54414088-54414107 MsG0280009565.01.T01:intron 20.0%
!! TGTCTTATAAAAATAACGAT+AGG - Chr2:54414157-54414176 MsG0280009565.01.T01:intron 20.0%
!! TTTACTATTAGTATTGTTGT+TGG + Chr2:54414127-54414146 None:intergenic 20.0%
!!! TATCAAAGTATTTACACAAT+AGG + Chr2:54413806-54413825 None:intergenic 20.0%
! AAATAACGATAGGATTGTTA+AGG - Chr2:54414167-54414186 MsG0280009565.01.T01:intron 25.0%
! AATTTCCTATGGAAAAGATT+AGG + Chr2:54413542-54413561 None:intergenic 25.0%
! ATATAATAGACCTGACTATA+TGG + Chr2:54413129-54413148 None:intergenic 25.0%
! ATTTCCTATGGAAAAGATTA+GGG + Chr2:54413541-54413560 None:intergenic 25.0%
! CAAAGATGCATAGATAAATT+AGG + Chr2:54414052-54414071 None:intergenic 25.0%
! GTCAAATAAAGTCAAAAGTA+AGG + Chr2:54414286-54414305 None:intergenic 25.0%
!! ATTAAGTTGTTTATCCAAAC+AGG + Chr2:54413589-54413608 None:intergenic 25.0%
!! ATTTTTATAAGACACATGAC+AGG + Chr2:54414151-54414170 None:intergenic 25.0%
AAGCTTTCTTGGAAAAGTTA+TGG - Chr2:54413707-54413726 MsG0280009565.01.T01:intron 30.0%
AAGGTTTGAACATCTTTCTA+TGG - Chr2:54413367-54413386 MsG0280009565.01.T01:three_prime_UTR 30.0%
TAGCGATTTACAATTTCCTA+TGG + Chr2:54413553-54413572 None:intergenic 30.0%
TTGGCAATGATAAAATAGTC+TGG + Chr2:54413269-54413288 None:intergenic 30.0%
! GCAATTTTCATAAGCTTTCT+TGG - Chr2:54413696-54413715 MsG0280009565.01.T01:intron 30.0%
! TGAAGATTTTATCCTTTGAC+AGG - Chr2:54413017-54413036 MsG0280009565.01.T01:three_prime_UTR 30.0%
! TTTGCCATTCAGATACTTTT+TGG + Chr2:54413992-54414011 None:intergenic 30.0%
! TTTTACCTGTAGATGTTAGA+TGG - Chr2:54413294-54413313 MsG0280009565.01.T01:three_prime_UTR 30.0%
!! AAAAGTATCTGAATGGCAAA+AGG - Chr2:54413992-54414011 MsG0280009565.01.T01:intron 30.0%
!!! GATTGACCTTTTTTGTTACA+GGG - Chr2:54413855-54413874 MsG0280009565.01.T01:CDS 30.0%
!!! TGATTGACCTTTTTTGTTAC+AGG - Chr2:54413854-54413873 MsG0280009565.01.T01:CDS 30.0%
AAGTGTACTTGCATTGAAAG+TGG - Chr2:54414239-54414258 MsG0280009565.01.T01:intron 35.0%
AATTCCATCAAACCTGTCAA+AGG + Chr2:54413032-54413051 None:intergenic 35.0%
ATATGGTGTACCTGAGAAAA+GGG + Chr2:54413112-54413131 None:intergenic 35.0%
ATCTACAGGTAAAAGACGTT+TGG + Chr2:54413288-54413307 None:intergenic 35.0%
TAAATCGCTAATCTGTGCTT+GGG - Chr2:54413563-54413582 MsG0280009565.01.T01:intron 35.0%
TAACAATAACGATATCGGAC+AGG + Chr2:54413200-54413219 None:intergenic 35.0%
TAACGATAGGATTGTTAAGG+AGG - Chr2:54414170-54414189 MsG0280009565.01.T01:intron 35.0%
TATATGGTGTACCTGAGAAA+AGG + Chr2:54413113-54413132 None:intergenic 35.0%
TGAAGACCCTGTAACAAAAA+AGG + Chr2:54413864-54413883 None:intergenic 35.0%
TTATCCTTTGACAGGTTTGA+TGG - Chr2:54413025-54413044 MsG0280009565.01.T01:three_prime_UTR 35.0%
! CGCATATGAATCAGCTATTT+TGG - Chr2:54413628-54413647 MsG0280009565.01.T01:intron 35.0%
! TCTTCCCTAATCTTTTCCAT+AGG - Chr2:54413534-54413553 MsG0280009565.01.T01:intron 35.0%
AAGAAGCGGTTTGATCAAGA+AGG - Chr2:54413348-54413367 MsG0280009565.01.T01:three_prime_UTR 40.0%
AAGAGATACCTGCAAGTCAA+AGG + Chr2:54413444-54413463 None:intergenic 40.0%
AGAGATACCTGCAAGTCAAA+GGG + Chr2:54413443-54413462 None:intergenic 40.0%
GGAAAAGATTAGGGAAGAGA+CGG + Chr2:54413532-54413551 None:intergenic 40.0%
GTAAATCGCTAATCTGTGCT+TGG - Chr2:54413562-54413581 MsG0280009565.01.T01:intron 40.0%
GTCCATGATCCATCATAAAC+CGG + Chr2:54413909-54413928 None:intergenic 40.0%
GTGAACCATCTAACATCTAC+AGG + Chr2:54413302-54413321 None:intergenic 40.0%
TAGGATTGTTAAGGAGGTTG+TGG - Chr2:54414176-54414195 MsG0280009565.01.T01:intron 40.0%
TCCATCATAAACCGGAACAT+CGG + Chr2:54413901-54413920 None:intergenic 40.0%
TTAGGTGAGCTCAGAATGTT+TGG + Chr2:54414034-54414053 None:intergenic 40.0%
! CAAATGCATTCCCTTTTCTC+AGG - Chr2:54413099-54413118 MsG0280009565.01.T01:three_prime_UTR 40.0%
! TGATGGATCATGGACTGAAT+GGG - Chr2:54413914-54413933 MsG0280009565.01.T01:intron 40.0%
!! ATGATGGATCATGGACTGAA+TGG - Chr2:54413913-54413932 MsG0280009565.01.T01:intron 40.0%
!! GCCACCAAAAAGTATCTGAA+TGG - Chr2:54413985-54414004 MsG0280009565.01.T01:intron 40.0%
!! GCCATTCAGATACTTTTTGG+TGG + Chr2:54413989-54414008 None:intergenic 40.0%
!! TTCCGGTTTATGATGGATCA+TGG - Chr2:54413904-54413923 MsG0280009565.01.T01:intron 40.0%
!! TTGACTTGCAGGTATCTCTT+TGG - Chr2:54413444-54413463 MsG0280009565.01.T01:CDS 40.0%
AACTGCATGCATTCTTGCAC+TGG - Chr2:54413501-54413520 MsG0280009565.01.T01:intron 45.0%
ACCGATGTTCCGGTTTATGA+TGG - Chr2:54413897-54413916 MsG0280009565.01.T01:CDS 45.0%
CTCAGGTACACCATATAGTC+AGG - Chr2:54413116-54413135 MsG0280009565.01.T01:intron 45.0%
GAGCATGTGACGGTTGAATT+CGG + Chr2:54413421-54413440 None:intergenic 45.0%
TCTTGGAAAGACCGATGTTC+CGG - Chr2:54413887-54413906 MsG0280009565.01.T01:CDS 45.0%
TTACAGGGTCTTCATCGTCT+TGG - Chr2:54413870-54413889 MsG0280009565.01.T01:CDS 45.0%
! AAGTCAAAGGGAGCATGTGA+CGG + Chr2:54413431-54413450 None:intergenic 45.0%
! AGGTATCTCTTTGGAAAGCC+CGG - Chr2:54413453-54413472 MsG0280009565.01.T01:CDS 45.0%
! GATGGATCATGGACTGAATG+GGG - Chr2:54413915-54413934 MsG0280009565.01.T01:intron 45.0%
! GTGGCTCTATTGCGTTTGTT+CGG + Chr2:54413970-54413989 None:intergenic 45.0%
AATCTGTGCTTGGGCCTGTT+TGG - Chr2:54413572-54413591 MsG0280009565.01.T01:intron 50.0%
AATTGCACCAGAGCCCAGAA+AGG - Chr2:54413048-54413067 MsG0280009565.01.T01:three_prime_UTR 50.0%
ACCACTCCTGATTCCCTTTC+TGG + Chr2:54413065-54413084 None:intergenic 50.0%
ATCAGGAGTGGTCATGTACC+TGG - Chr2:54413073-54413092 MsG0280009565.01.T01:three_prime_UTR 50.0%
ATTGCACCAGAGCCCAGAAA+GGG - Chr2:54413049-54413068 MsG0280009565.01.T01:three_prime_UTR 50.0%
CAGCAGACGAGCTTAAGAAG+CGG - Chr2:54413334-54413353 MsG0280009565.01.T01:three_prime_UTR 50.0%
CCACTCCTGATTCCCTTTCT+GGG + Chr2:54413064-54413083 None:intergenic 50.0%
GCTCTATTGCGTTTGTTCGG+CGG + Chr2:54413967-54413986 None:intergenic 50.0%
GTTCCACACGAAGTCACAAC+CGG + Chr2:54413475-54413494 None:intergenic 50.0%
GTTGTGACTTCGTGTGGAAC+TGG - Chr2:54413475-54413494 MsG0280009565.01.T01:CDS 50.0%
TTCCACACGAAGTCACAACC+GGG + Chr2:54413474-54413493 None:intergenic 50.0%
! AAGGGAATGCATTTGCTGCC+AGG + Chr2:54413094-54413113 None:intergenic 50.0%
!! ACATGCTCCCTTTGACTTGC+AGG - Chr2:54413433-54413452 MsG0280009565.01.T01:CDS 50.0%
CAGAGCCCAGAAAGGGAATC+AGG - Chr2:54413056-54413075 MsG0280009565.01.T01:three_prime_UTR 55.0%
CCCAGAAAGGGAATCAGGAG+TGG - Chr2:54413061-54413080 MsG0280009565.01.T01:three_prime_UTR 55.0%
CGCTTCTTAAGCTCGTCTGC+TGG + Chr2:54413336-54413355 None:intergenic 55.0%
CTGATTCCCTTTCTGGGCTC+TGG + Chr2:54413058-54413077 None:intergenic 55.0%
! GCGGTGTCAACAGGTGTATC+AGG + Chr2:54413948-54413967 None:intergenic 55.0%
! GTTTGTTCGGCGGTGTCAAC+AGG + Chr2:54413957-54413976 None:intergenic 55.0%
AGCCCGGTTGTGACTTCGTG+TGG - Chr2:54413469-54413488 MsG0280009565.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 54413001 54414370 54413001 ID=MsG0280009565.01;Name=MsG0280009565.01
Chr2 mRNA 54413001 54414370 54413001 ID=MsG0280009565.01.T01;Parent=MsG0280009565.01;Name=MsG0280009565.01.T01;_AED=0.45;_eAED=0.48;_QI=0|1|0.8|1|0.75|0.6|5|343|119
Chr2 exon 54413174 54413495 54413174 ID=MsG0280009565.01.T01:exon:38098;Parent=MsG0280009565.01.T01
Chr2 exon 54413001 54413129 54413001 ID=MsG0280009565.01.T01:exon:38099;Parent=MsG0280009565.01.T01
Chr2 CDS 54414251 54414370 54414251 ID=MsG0280009565.01.T01:cds;Parent=MsG0280009565.01.T01
Chr2 CDS 54414002 54414065 54414002 ID=MsG0280009565.01.T01:cds;Parent=MsG0280009565.01.T01
Chr2 CDS 54413849 54413916 54413849 ID=MsG0280009565.01.T01:cds;Parent=MsG0280009565.01.T01
Chr2 CDS 54413388 54413495 54413388 ID=MsG0280009565.01.T01:cds;Parent=MsG0280009565.01.T01
Chr2 three_prime_UTR 54413174 54413387 54413174 ID=MsG0280009565.01.T01:three_prime_utr;Parent=MsG0280009565.01.T01
Chr2 three_prime_UTR 54413001 54413129 54413001 ID=MsG0280009565.01.T01:three_prime_utr;Parent=MsG0280009565.01.T01
Chr2 mRNA 54413002 54414370 54413002 ID=MsG0280009565.01.T02;Parent=MsG0280009565.01;Name=MsG0280009565.01.T02;_AED=0.45;_eAED=0.48;_QI=0|1|0.75|1|1|1|4|386|119
Chr2 exon 54413002 54413495 54413002 ID=MsG0280009565.01.T02:exon:38100;Parent=MsG0280009565.01.T02
Chr2 CDS 54414251 54414370 54414251 ID=MsG0280009565.01.T02:cds;Parent=MsG0280009565.01.T02
Chr2 CDS 54414002 54414065 54414002 ID=MsG0280009565.01.T02:cds;Parent=MsG0280009565.01.T02
Chr2 CDS 54413849 54413916 54413849 ID=MsG0280009565.01.T02:cds;Parent=MsG0280009565.01.T02
Chr2 CDS 54413388 54413495 54413388 ID=MsG0280009565.01.T02:cds;Parent=MsG0280009565.01.T02
Chr2 three_prime_UTR 54413002 54413387 54413002 ID=MsG0280009565.01.T02:three_prime_utr;Parent=MsG0280009565.01.T02
Gene Sequence

>MsG0280009565.01.T01

ATGGCTCTTGGATTTCTGAAGATTTTATCCTTTGACAGGTTTGATGGAATTGCACCAGAGCCCAGAAAGGGAATCAGGAGTGGTCATGTACCTGGCAGCAAATGCATTCCCTTTTCTCAGATGTTAGATGGTTCACAGACATTGTTACCAGCAGACGAGCTTAAGAAGCGGTTTGATCAAGAAGGTATCTCTTTGGAAAGCCCGGTTGTGACTTCGTGTGGAACTGGAGTAACTGCATGCATTCTTGCACTGGGTCTTCATCGTCTTGGAAAGACCGATGTTCCGGTTTATGATGGATCATGGACTGAATGGGGAGCACATCCTGATACACCTGTTGACACCGCCGAACAAACGCAATAG

>MsG0280009565.01.T02

ATGGCTCTTGGATTTCTGAAGATTTTATCCTTTGACAGGTTTGATGGAATTGCACCAGAGCCCAGAAAGGGAATCAGGAGTGGTCATGTACCTGGCAGCAAATGCATTCCCTTTTCTCAGATGTTAGATGGTTCACAGACATTGTTACCAGCAGACGAGCTTAAGAAGCGGTTTGATCAAGAAGGTATCTCTTTGGAAAGCCCGGTTGTGACTTCGTGTGGAACTGGAGTAACTGCATGCATTCTTGCACTGGGTCTTCATCGTCTTGGAAAGACCGATGTTCCGGTTTATGATGGATCATGGACTGAATGGGGAGCACATCCTGATACACCTGTTGACACCGCCGAACAAACGCAATAG

Protein sequence

>MsG0280009565.01.T01

MALGFLKILSFDRFDGIAPEPRKGIRSGHVPGSKCIPFSQMLDGSQTLLPADELKKRFDQEGISLESPVVTSCGTGVTACILALGLHRLGKTDVPVYDGSWTEWGAHPDTPVDTAEQTQ*

>MsG0280009565.01.T02

MALGFLKILSFDRFDGIAPEPRKGIRSGHVPGSKCIPFSQMLDGSQTLLPADELKKRFDQEGISLESPVVTSCGTGVTACILALGLHRLGKTDVPVYDGSWTEWGAHPDTPVDTAEQTQ*