AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280011120.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011120.01.T01 MTR_2g099090 93.182 44 3 0 86 129 239 282 3.05e-23 92.8
MsG0280011120.01.T01 MTR_2g099090 93.182 44 3 0 86 129 223 266 3.31e-23 92.4
MsG0280011120.01.T01 MTR_4g068100 72.727 44 12 0 86 129 163 206 8.00e-17 74.7
MsG0280011120.01.T01 MTR_4g068100 72.727 44 12 0 86 129 225 268 1.19e-16 74.7
MsG0280011120.01.T01 MTR_4g068100 72.727 44 12 0 86 129 225 268 1.46e-16 74.7
MsG0280011120.01.T01 MTR_4g068100 72.727 44 12 0 86 129 233 276 1.47e-16 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011120.01.T01 AT2G31350 70.455 44 13 0 86 129 234 277 9.47e-15 69.7
MsG0280011120.01.T01 AT2G31350 70.455 44 13 0 86 129 233 276 9.60e-15 69.7
MsG0280011120.01.T01 AT1G06130 61.364 44 17 0 86 129 238 281 1.13e-12 63.9
MsG0280011120.01.T01 AT1G06130 61.364 44 17 0 86 129 239 282 1.14e-12 63.9

Find 45 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTTCTCATGTTGCATTAC+AGG 0.131265 2:-79772268 MsG0280011120.01.T01:intron
GGCTTGCCCACGGTGAGTTT+TGG 0.284331 2:-79770999 MsG0280011120.01.T01:CDS
ACATCCCACATCTTCAATTA+TGG 0.301100 2:-79771299 MsG0280011120.01.T01:CDS
GAGAAGATTGCATCTGTAAC+AGG 0.316777 2:+79771265 None:intergenic
GGCCACATCACTTCTACTTC+CGG 0.365579 2:-79772036 MsG0280011120.01.T01:CDS
GATCGAAGTTCCTTGTTTCC+AGG 0.387556 2:+79771056 None:intergenic
GCCACATCACTTCTACTTCC+GGG 0.396390 2:-79772035 MsG0280011120.01.T01:CDS
TCACTTCTACTTCCGGGATC+GGG 0.417247 2:-79772029 MsG0280011120.01.T01:CDS
TTACAGATGCAATCTTCTCT+TGG 0.426089 2:-79771261 MsG0280011120.01.T01:CDS
TCATGCGGCAAACTCTTTGA+AGG 0.453311 2:-79771973 MsG0280011120.01.T01:CDS
ATCACTTCTACTTCCGGGAT+CGG 0.463071 2:-79772030 MsG0280011120.01.T01:CDS
ACAGTATATATTGTATCGTC+TGG 0.465841 2:+79771222 None:intergenic
GTGGCTTCCCTTCGAAGTAA+AGG 0.472139 2:-79771020 MsG0280011120.01.T01:CDS
GTTGTCAAGTCAGTTATACT+TGG 0.473032 2:-79771333 MsG0280011120.01.T01:intron
TTCGCATTGAGGCTCGAACC+TGG 0.481254 2:-79771074 MsG0280011120.01.T01:CDS
ACCTGTAATGCAACATGAGA+AGG 0.489390 2:+79772267 None:intergenic
TTATGCCTGGTTATGGCAAA+GGG 0.504635 2:-79772306 None:intergenic
ATTATGCCTGGTTATGGCAA+AGG 0.510014 2:-79772307 None:intergenic
ACTCTCCATAATTGAAGATG+TGG 0.526987 2:+79771294 None:intergenic
AAGGAACTTCGATCCTATGC+TGG 0.529395 2:-79771047 MsG0280011120.01.T01:CDS
GGGCAAGCCTTTACTTCGAA+GGG 0.545894 2:+79771013 None:intergenic
GGAAGCCACGTGGCCAGCAT+AGG 0.547492 2:+79771034 None:intergenic
TGGGCAAGCCTTTACTTCGA+AGG 0.550848 2:+79771012 None:intergenic
CACTTCTACTTCCGGGATCG+GGG 0.561880 2:-79772028 MsG0280011120.01.T01:CDS
ACTCTTTGAAGGAACCCCTC+AGG 0.563265 2:-79771962 MsG0280011120.01.T01:CDS
AGGTAAAACCAACCTCCTGA+GGG 0.580185 2:+79771947 None:intergenic
CAGGTAAAACCAACCTCCTG+AGG 0.581515 2:+79771946 None:intergenic
CTCTCCATAATTGAAGATGT+GGG 0.586910 2:+79771295 None:intergenic
GAGGCTCGAACCTGGAAACA+AGG 0.587160 2:-79771066 MsG0280011120.01.T01:CDS
CCTGTAATGCAACATGAGAA+GGG 0.602438 2:+79772268 None:intergenic
CTTTGAAGGAACCCCTCAGG+AGG 0.623384 2:-79771959 MsG0280011120.01.T01:intron
TCCCGGAAGTAGAAGTGATG+TGG 0.630241 2:+79772034 None:intergenic
AGAAGATTGCATCTGTAACA+GGG 0.641756 2:+79771266 None:intergenic
ATAATACGGACTTCGCATTG+AGG 0.642456 2:-79771085 MsG0280011120.01.T01:CDS
CCAATTCCAAAACTCACCGT+GGG 0.643980 2:+79770993 None:intergenic
GCCAATTCCAAAACTCACCG+TGG 0.646189 2:+79770992 None:intergenic
TCGAAGTAAAGGCTTGCCCA+CGG 0.654918 2:-79771009 MsG0280011120.01.T01:CDS
AGACGATACAATATATACTG+TGG 0.659925 2:-79771220 MsG0280011120.01.T01:CDS
ACTTTGTTCAGCTTGTCATG+CGG 0.663144 2:-79771988 MsG0280011120.01.T01:CDS
CTGTGGACACGAGTACACAT+TGG 0.683875 2:-79771203 MsG0280011120.01.T01:intron
TCGATCCTATGCTGGCCACG+TGG 0.686304 2:-79771039 MsG0280011120.01.T01:CDS
TACTTCGAAGGGAAGCCACG+TGG 0.689024 2:+79771024 None:intergenic
ACTACTCATAAAGAATAACA+AGG 0.704151 2:+79770967 None:intergenic
GGTAAAACCAACCTCCTGAG+GGG 0.722369 2:+79771948 None:intergenic
AGATTGCATCTGTAACAGGG+AGG 0.764846 2:+79771269 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAATGAGCATAATTTTTA+GGG - Chr2:79771512-79771531 MsG0280011120.01.T01:intron 15.0%
!! ATAAAAATACTGAGTTCATT+CGG - Chr2:79771750-79771769 MsG0280011120.01.T01:intron 20.0%
!! ATTATGCTCATTAAAGATAT+TGG + Chr2:79771507-79771526 None:intergenic 20.0%
!!! CTTTAATGAGCATAATTTTT+AGG - Chr2:79771511-79771530 MsG0280011120.01.T01:intron 20.0%
!!! TGTATTTTTGCAGAATAATA+CGG - Chr2:79772163-79772182 MsG0280011120.01.T01:intron 20.0%
! ATTTACTTGCATTTACAACT+AGG + Chr2:79771901-79771920 None:intergenic 25.0%
! TGTTTATTTATACTGTAAGC+AGG - Chr2:79771205-79771224 MsG0280011120.01.T01:CDS 25.0%
AAAATGTTATTGCTGATGCA+AGG - Chr2:79771875-79771894 MsG0280011120.01.T01:intron 30.0%
AAATAAAGCAAAGAACACTG+AGG + Chr2:79772105-79772124 None:intergenic 30.0%
ACAGTATATATTGTATCGTC+TGG + Chr2:79772043-79772062 None:intergenic 30.0%
AGACGATACAATATATACTG+TGG - Chr2:79772042-79772061 MsG0280011120.01.T01:intron 30.0%
CATTGATATCCATTGAATGA+CGG - Chr2:79771054-79771073 MsG0280011120.01.T01:CDS 30.0%
GGTTTCACATAATGCAAATA+AGG + Chr2:79771154-79771173 None:intergenic 30.0%
TATGTATGTCGAAAAATGCT+TGG - Chr2:79771822-79771841 MsG0280011120.01.T01:intron 30.0%
TTTCAAATTTCAAGGACTTC+TGG + Chr2:79771427-79771446 None:intergenic 30.0%
! ATACAAAGTACTAGAGTCAA+TGG + Chr2:79772148-79772167 None:intergenic 30.0%
!!! CATAATTTTTAGGGCTTGTT+GGG - Chr2:79771521-79771540 MsG0280011120.01.T01:intron 30.0%
AAAAAAGACAAGTTAGTGCC+AGG + Chr2:79771338-79771357 None:intergenic 35.0%
AATTTGAAAATACCCGCACA+AGG - Chr2:79771438-79771457 MsG0280011120.01.T01:intron 35.0%
ACATCCCACATCTTCAATTA+TGG - Chr2:79771963-79771982 MsG0280011120.01.T01:CDS 35.0%
ACTCTCCATAATTGAAGATG+TGG + Chr2:79771971-79771990 None:intergenic 35.0%
AGAAGATTGCATCTGTAACA+GGG + Chr2:79771999-79772018 None:intergenic 35.0%
ATTCAATGGATATCAATGCC+AGG + Chr2:79771052-79771071 None:intergenic 35.0%
ATTTAAATTGCAGCCAAACG+AGG - Chr2:79771469-79771488 MsG0280011120.01.T01:intron 35.0%
CGCTGTAATAATATGAGAGT+GGG - Chr2:79771545-79771564 MsG0280011120.01.T01:intron 35.0%
CTCGTTGATATGTCTTCTTA+AGG - Chr2:79771784-79771803 MsG0280011120.01.T01:intron 35.0%
CTCTCCATAATTGAAGATGT+GGG + Chr2:79771970-79771989 None:intergenic 35.0%
GTTGTCAAGTCAGTTATACT+TGG - Chr2:79771929-79771948 MsG0280011120.01.T01:intron 35.0%
TAAATCACAAGTTCCTTGTG+CGG + Chr2:79771454-79771473 None:intergenic 35.0%
TACACTAAGATTGCAGACTT+GGG + Chr2:79771625-79771644 None:intergenic 35.0%
TCGCTGTAATAATATGAGAG+TGG - Chr2:79771544-79771563 MsG0280011120.01.T01:intron 35.0%
TTACAGATGCAATCTTCTCT+TGG - Chr2:79772001-79772020 MsG0280011120.01.T01:CDS 35.0%
! GCGGGTATTTTCAAATTTCA+AGG + Chr2:79771435-79771454 None:intergenic 35.0%
!!! GCATAATTTTTAGGGCTTGT+TGG - Chr2:79771520-79771539 MsG0280011120.01.T01:intron 35.0%
AAATCACAAGTTCCTTGTGC+GGG + Chr2:79771453-79771472 None:intergenic 40.0%
AAGTACTAGAGTCAATGGCA+CGG + Chr2:79772143-79772162 None:intergenic 40.0%
AATTTCAAGGACTTCTGGCA+AGG + Chr2:79771422-79771441 None:intergenic 40.0%
ACACTAAGATTGCAGACTTG+GGG + Chr2:79771624-79771643 None:intergenic 40.0%
ACCTGTAATGCAACATGAGA+AGG + Chr2:79770998-79771017 None:intergenic 40.0%
ACTTTGTTCAGCTTGTCATG+CGG - Chr2:79771274-79771293 MsG0280011120.01.T01:CDS 40.0%
ATAATACGGACTTCGCATTG+AGG - Chr2:79772177-79772196 MsG0280011120.01.T01:intron 40.0%
ATTTCAAGGACTTCTGGCAA+GGG + Chr2:79771421-79771440 None:intergenic 40.0%
CACTTATCACCGTCATTCAA+TGG + Chr2:79771066-79771085 None:intergenic 40.0%
CATGTTGCATTACAGGTGAT+TGG - Chr2:79771001-79771020 MsG0280011120.01.T01:CDS 40.0%
CATTGAATGACGGTGATAAG+TGG - Chr2:79771064-79771083 MsG0280011120.01.T01:CDS 40.0%
CCTGTAATGCAACATGAGAA+GGG + Chr2:79770997-79771016 None:intergenic 40.0%
GAGAAGATTGCATCTGTAAC+AGG + Chr2:79772000-79772019 None:intergenic 40.0%
GTACACTAAGATTGCAGACT+TGG + Chr2:79771626-79771645 None:intergenic 40.0%
TGCAATCTTAGTGTACGAGA+AGG - Chr2:79771630-79771649 MsG0280011120.01.T01:intron 40.0%
! CACGTGTATGAAGTGTTCAA+TGG - Chr2:79771677-79771696 MsG0280011120.01.T01:intron 40.0%
! TCATACACGTGCTGATCAAA+GGG + Chr2:79771668-79771687 None:intergenic 40.0%
! TGTGAAACCTTCTTGTTGTG+TGG - Chr2:79771165-79771184 MsG0280011120.01.T01:intron 40.0%
! TTCATACACGTGCTGATCAA+AGG + Chr2:79771669-79771688 None:intergenic 40.0%
AAGGAACTTCGATCCTATGC+TGG - Chr2:79772215-79772234 MsG0280011120.01.T01:intron 45.0%
ACCGACAACAAAAGGATTCC+TGG - Chr2:79771031-79771050 MsG0280011120.01.T01:CDS 45.0%
AGAAGAGCCACACAACAAGA+AGG + Chr2:79771175-79771194 None:intergenic 45.0%
AGAGTTTGAGTGTAGTGCGA+TGG - Chr2:79771577-79771596 MsG0280011120.01.T01:intron 45.0%
AGATTGCATCTGTAACAGGG+AGG + Chr2:79771996-79772015 None:intergenic 45.0%
AGGTAAAACCAACCTCCTGA+GGG + Chr2:79771318-79771337 None:intergenic 45.0%
AGTCAATGGCACGGTACTTA+GGG + Chr2:79772134-79772153 None:intergenic 45.0%
ATCACTTCTACTTCCGGGAT+CGG - Chr2:79771232-79771251 MsG0280011120.01.T01:CDS 45.0%
CACTAAGATTGCAGACTTGG+GGG + Chr2:79771623-79771642 None:intergenic 45.0%
CCAATTCCAAAACTCACCGT+GGG + Chr2:79772272-79772291 None:intergenic 45.0%
CCCTTCTCATGTTGCATTAC+AGG - Chr2:79770994-79771013 MsG0280011120.01.T01:CDS 45.0%
GGTGATAAGTGGATGTTTGC+TGG - Chr2:79771075-79771094 MsG0280011120.01.T01:CDS 45.0%
GTTCAGCAACCGACAACAAA+AGG - Chr2:79771023-79771042 MsG0280011120.01.T01:CDS 45.0%
TCATGCGGCAAACTCTTTGA+AGG - Chr2:79771289-79771308 MsG0280011120.01.T01:CDS 45.0%
TGTAAAAATTGCCCCGATCC+CGG + Chr2:79771248-79771267 None:intergenic 45.0%
TGTCCATTACTTGCACCTCA+TGG + Chr2:79771101-79771120 None:intergenic 45.0%
! CATACACGTGCTGATCAAAG+GGG + Chr2:79771667-79771686 None:intergenic 45.0%
! GATCGAAGTTCCTTGTTTCC+AGG + Chr2:79772209-79772228 None:intergenic 45.0%
!! GCCAGGAATCCTTTTGTTGT+CGG + Chr2:79771035-79771054 None:intergenic 45.0%
CAGGTAAAACCAACCTCCTG+AGG + Chr2:79771319-79771338 None:intergenic 50.0%
CTGTGGACACGAGTACACAT+TGG - Chr2:79772059-79772078 MsG0280011120.01.T01:intron 50.0%
GAGTCAATGGCACGGTACTT+AGG + Chr2:79772135-79772154 None:intergenic 50.0%
GCCAATTCCAAAACTCACCG+TGG + Chr2:79772273-79772292 None:intergenic 50.0%
GCCACATCACTTCTACTTCC+GGG - Chr2:79771227-79771246 MsG0280011120.01.T01:CDS 50.0%
GGCCACATCACTTCTACTTC+CGG - Chr2:79771226-79771245 MsG0280011120.01.T01:CDS 50.0%
GGGCAAGCCTTTACTTCGAA+GGG + Chr2:79772252-79772271 None:intergenic 50.0%
GGTAAAACCAACCTCCTGAG+GGG + Chr2:79771317-79771336 None:intergenic 50.0%
GTCACCACAAAGTCCTCGTT+TGG + Chr2:79771485-79771504 None:intergenic 50.0%
GTGGCTTCCCTTCGAAGTAA+AGG - Chr2:79772242-79772261 MsG0280011120.01.T01:intron 50.0%
TCACTTCTACTTCCGGGATC+GGG - Chr2:79771233-79771252 MsG0280011120.01.T01:CDS 50.0%
TCCCGGAAGTAGAAGTGATG+TGG + Chr2:79771231-79771250 None:intergenic 50.0%
TCGAAGTAAAGGCTTGCCCA+CGG - Chr2:79772253-79772272 MsG0280011120.01.T01:intron 50.0%
TGGCCATGAGGTGCAAGTAA+TGG - Chr2:79771095-79771114 MsG0280011120.01.T01:intron 50.0%
TGGGCAAGCCTTTACTTCGA+AGG + Chr2:79772253-79772272 None:intergenic 50.0%
! ACTCTTTGAAGGAACCCCTC+AGG - Chr2:79771300-79771319 MsG0280011120.01.T01:CDS 50.0%
! GGGATCGGGGCAATTTTTAC+AGG - Chr2:79771247-79771266 MsG0280011120.01.T01:CDS 50.0%
!! GTGCAAGTAATGGACACACC+TGG - Chr2:79771105-79771124 MsG0280011120.01.T01:intron 50.0%
!!! CCCACGGTGAGTTTTGGAAT+TGG - Chr2:79772269-79772288 MsG0280011120.01.T01:CDS 50.0%
!!! CTCAGGAGGTTGGTTTTACC+TGG - Chr2:79771317-79771336 MsG0280011120.01.T01:CDS 50.0%
ATGTGACCTCGAGTGTGACC+AGG + Chr2:79771126-79771145 None:intergenic 55.0%
CACTTCTACTTCCGGGATCG+GGG - Chr2:79771234-79771253 MsG0280011120.01.T01:CDS 55.0%
GAGGCTCGAACCTGGAAACA+AGG - Chr2:79772196-79772215 MsG0280011120.01.T01:intron 55.0%
GCAGCCAAACGAGGACTTTG+TGG - Chr2:79771478-79771497 MsG0280011120.01.T01:intron 55.0%
TACTTCGAAGGGAAGCCACG+TGG + Chr2:79772241-79772260 None:intergenic 55.0%
TTCGCATTGAGGCTCGAACC+TGG - Chr2:79772188-79772207 MsG0280011120.01.T01:intron 55.0%
! CTTTGAAGGAACCCCTCAGG+AGG - Chr2:79771303-79771322 MsG0280011120.01.T01:CDS 55.0%
! GTGGATGTTTGCTGGCCATG+AGG - Chr2:79771083-79771102 MsG0280011120.01.T01:CDS 55.0%
GAAGGAACCCCTCAGGAGGT+TGG - Chr2:79771307-79771326 MsG0280011120.01.T01:CDS 60.0%
GACACACCTGGTCACACTCG+AGG - Chr2:79771117-79771136 MsG0280011120.01.T01:intron 60.0%
TCGATCCTATGCTGGCCACG+TGG - Chr2:79772223-79772242 MsG0280011120.01.T01:intron 60.0%
! GGCTTGCCCACGGTGAGTTT+TGG - Chr2:79772263-79772282 MsG0280011120.01.T01:intron 60.0%
GGAAGCCACGTGGCCAGCAT+AGG + Chr2:79772231-79772250 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 79770968 79772316 79770968 ID=MsG0280011120.01;Name=MsG0280011120.01
Chr2 mRNA 79770968 79772316 79770968 ID=MsG0280011120.01.T01;Parent=MsG0280011120.01;Name=MsG0280011120.01.T01;_AED=0.47;_eAED=0.55;_QI=0|0.33|0.25|1|0.66|0.75|4|0|141
Chr2 exon 79772269 79772316 79772269 ID=MsG0280011120.01.T01:exon:10116;Parent=MsG0280011120.01.T01
Chr2 exon 79771960 79772057 79771960 ID=MsG0280011120.01.T01:exon:10115;Parent=MsG0280011120.01.T01
Chr2 exon 79771204 79771342 79771204 ID=MsG0280011120.01.T01:exon:10114;Parent=MsG0280011120.01.T01
Chr2 exon 79770968 79771108 79770968 ID=MsG0280011120.01.T01:exon:10113;Parent=MsG0280011120.01.T01
Chr2 CDS 79772269 79772316 79772269 ID=MsG0280011120.01.T01:cds;Parent=MsG0280011120.01.T01
Chr2 CDS 79771960 79772057 79771960 ID=MsG0280011120.01.T01:cds;Parent=MsG0280011120.01.T01
Chr2 CDS 79771204 79771342 79771204 ID=MsG0280011120.01.T01:cds;Parent=MsG0280011120.01.T01
Chr2 CDS 79770968 79771108 79770968 ID=MsG0280011120.01.T01:cds;Parent=MsG0280011120.01.T01
Gene Sequence

>MsG0280011120.01.T01

ATGGCAAAGGGCTGTGTAAATGTTAACCCTTCTCATGTTGCATTACAGGCCACATCACTTCTACTTCCGGGATCGGGGCAATTTTTACAGGAGACACTTTGTTCAGCTTGTCATGCGGCAAACTCTTTGAAGGAACCCCTCAGGAGTTATACTTGGAAAGCTTTAGAACATCCCACATCTTCAATTATGGAGAGTTTCCTCCCTGTTACAGATGCAATCTTCTCTTGGAAAGATCATGTCTTTACCAGACGATACAATATATACTGTGGACACGAGTACACATTGAATAATACGGACTTCGCATTGAGGCTCGAACCTGGAAACAAGGAACTTCGATCCTATGCTGGCCACGTGGCTTCCCTTCGAAGTAAAGGCTTGCCCACGGTGAGTTTTGGAATTGGCTTCCTTGTTATTCTTTATGAGTAG

Protein sequence

>MsG0280011120.01.T01

MAKGCVNVNPSHVALQATSLLLPGSGQFLQETLCSACHAANSLKEPLRSYTWKALEHPTSSIMESFLPVTDAIFSWKDHVFTRRYNIYCGHEYTLNNTDFALRLEPGNKELRSYAGHVASLRSKGLPTVSFGIGFLVILYE*