AlfalfaGEDB | MsG0280010695.01

M. sativa cultivar ZhongmuNo.1 / MsG0280010695.01

KEGG: K03644
GO: GO:0003824 GO:0009107 GO:0016992 GO:0051536 GO:0051539
PFAM: PF04055

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010695.01.T01	XP_013465052.1	85.496	262	7	1	1	231	117	378	2.58E-149	431
MsG0280010695.01.T02	XP_013465052.1	85.496	262	7	1	1	231	117	378	2.58E-149	431

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010695.01.T01	B7FM45	85.115	262	8	1	1	231	117	378	4.84E-151	427
MsG0280010695.01.T02	B7FM45	85.115	262	8	1	1	231	117	378	4.84E-151	427

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010695.01.T01	A0A072VBA9	85.496	262	7	1	1	231	117	378	1.23e-149	431
MsG0280010695.01.T02	A0A072VBA9	85.496	262	7	1	1	231	117	378	1.23e-149	431

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047967.01	MsG0280010695.01	0.827734	1.328329e-54	1.293386e-51
MsG0080048690.01	MsG0280010695.01	0.801475	8.849692e-49	4.230243e-46
MsG0080048692.01	MsG0280010695.01	0.899562	1.752784e-77	2.179287e-73
MsG0080048693.01	MsG0280010695.01	0.804643	1.957927e-49	1.014846e-46
MsG0080048694.01	MsG0280010695.01	0.829402	5.251075e-55	5.369079e-52
MsG0080048695.01	MsG0280010695.01	0.828410	9.131263e-55	9.069024e-52
MsG0080048697.01	MsG0280010695.01	0.815030	1.138994e-51	7.765795e-49
MsG0080048698.01	MsG0280010695.01	0.841551	4.436627e-58	6.542193e-55
MsG0080048699.01	MsG0280010695.01	0.868162	8.016242e-66	2.871250e-62
MsG0080048754.01	MsG0280010695.01	0.816745	4.719404e-52	3.372280e-49

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010695.01.T01	MTR_2g090140	85.496	262	7	1	1	231	117	378	3.12e-153	431
MsG0280010695.01.T01	MTR_1g050432	53.386	251	87	1	1	221	127	377	3.52e-97	289
MsG0280010695.01.T02	MTR_2g090140	85.496	262	7	1	1	231	117	378	3.12e-153	431
MsG0280010695.01.T02	MTR_1g050432	53.386	251	87	1	1	221	127	377	3.52e-97	289

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010695.01.T01	AT2G20860	76.834	259	29	1	1	228	111	369	1.24e-136	389
MsG0280010695.01.T01	AT2G20860	76.834	259	29	1	1	228	111	369	1.24e-136	389
MsG0280010695.01.T01	AT2G20860	76.834	259	29	1	1	228	111	369	1.24e-136	389
MsG0280010695.01.T01	AT5G08415	55.556	252	82	2	1	222	135	386	3.95e-90	271
MsG0280010695.01.T02	AT2G20860	76.834	259	29	1	1	228	111	369	1.24e-136	389
MsG0280010695.01.T02	AT2G20860	76.834	259	29	1	1	228	111	369	1.24e-136	389
MsG0280010695.01.T02	AT2G20860	76.834	259	29	1	1	228	111	369	1.24e-136	389
MsG0280010695.01.T02	AT5G08415	55.556	252	82	2	1	222	135	386	3.95e-90	271

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Find 17 sgRNAs with CRISPR-Local

Find 312 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATCCATGATTAATTCAGAT+CGG	0.512241	2:+73485665	MsG0280010695.01.T01:CDS
AGCCCGATCTGAATTAATCA+TGG	0.510455	2:-73485668	None:intergenic
ATATGTGGCATCCGGGCCCA+TGG	0.566837	2:+73485606	MsG0280010695.01.T01:CDS
ATCCATGATTAATTCAGATC+GGG	0.456945	2:+73485666	MsG0280010695.01.T01:CDS
ATTAAGATGGTATTAATGAG+TGG	0.510060	2:+73485830	MsG0280010695.01.T02:three_prime_UTR
CATGGTAAGGTCATCATACA+AGG	0.536106	2:+73485624	MsG0280010695.01.T01:CDS
CATGTAATCTATCATTAAGA+TGG	0.518940	2:+73485817	MsG0280010695.01.T01:three_prime_UTR
CTTGTATGATGACCTTACCA+TGG	0.582701	2:-73485623	None:intergenic
GATTTCGATATGTGGCATCC+GGG	0.563544	2:+73485599	MsG0280010695.01.T01:CDS
GGATTTCGATATGTGGCATC+CGG	0.456753	2:+73485598	MsG0280010695.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATAGTCTGATACTTCTCAA+AGG	-	Chr2:73484945-73484964	None:intergenic	30.0%
AATATTTGGTCCACAATACA+AGG	+	Chr2:73485161-73485180	MsG0280010695.01.T01:intron	30.0%
AATCATAATAGTAGCAGTAG+CGG	-	Chr2:73482041-73482060	None:intergenic	30.0%
ACCAAGACTTCAATAATGTT+AGG	+	Chr2:73484785-73484804	MsG0280010695.01.T01:intron	30.0%
ACCTAACATTATTGAAGTCT+TGG	-	Chr2:73484789-73484808	None:intergenic	30.0%
ACTGCTACTATTATGATTCT+TGG	+	Chr2:73482043-73482062	MsG0280010695.01.T01:CDS	30.0%
ACTTCAATAATGTTAGGTGT+GGG	+	Chr2:73484791-73484810	MsG0280010695.01.T01:intron	30.0%
AGTGCTAATTACATTCATCT+AGG	+	Chr2:73483095-73483114	MsG0280010695.01.T01:intron	30.0%
ATAAACTATATACTCTCACG+GGG	-	Chr2:73483620-73483639	None:intergenic	30.0%
ATAAGCAAGCTGTATTACTT+TGG	+	Chr2:73482363-73482382	MsG0280010695.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	73481567	73485905	73481567	ID=MsG0280010695.01;Name=MsG0280010695.01
Chr2	mRNA	73481567	73485845	73481567	ID=MsG0280010695.01.T01;Parent=MsG0280010695.01;Name=MsG0280010695.01.T01;_AED=0.36;_eAED=0.36;_QI=437\|0.8\|0.66\|1\|0.8\|0.66\|6\|107\|231
Chr2	exon	73485598	73485735	73485598	ID=MsG0280010695.01.T01:exon:7275;Parent=MsG0280010695.01.T01
Chr2	exon	73485766	73485845	73485766	ID=MsG0280010695.01.T01:exon:7276;Parent=MsG0280010695.01.T01
Chr2	five_prime_UTR	73481567	73482003	73481567	ID=MsG0280010695.01.T01:five_prime_utr;Parent=MsG0280010695.01.T01
Chr2	CDS	73482004	73482089	73482004	ID=MsG0280010695.01.T01:cds;Parent=MsG0280010695.01.T01
Chr2	CDS	73483848	73484059	73483848	ID=MsG0280010695.01.T01:cds;Parent=MsG0280010695.01.T01
Chr2	CDS	73484601	73484717	73484601	ID=MsG0280010695.01.T01:cds;Parent=MsG0280010695.01.T01
Chr2	CDS	73484807	73484976	73484807	ID=MsG0280010695.01.T01:cds;Parent=MsG0280010695.01.T01
Chr2	CDS	73485598	73485708	73485598	ID=MsG0280010695.01.T01:cds;Parent=MsG0280010695.01.T01
Chr2	three_prime_UTR	73485709	73485735	73485709	ID=MsG0280010695.01.T01:three_prime_utr;Parent=MsG0280010695.01.T01
Chr2	three_prime_UTR	73485766	73485845	73485766	ID=MsG0280010695.01.T01:three_prime_utr;Parent=MsG0280010695.01.T01
Chr2	mRNA	73481567	73485905	73481567	ID=MsG0280010695.01.T02;Parent=MsG0280010695.01;Name=MsG0280010695.01.T02;_AED=0.35;_eAED=0.35;_QI=437\|0.75\|0.6\|1\|1\|1\|5\|197\|231
Chr2	exon	73485598	73485905	73485598	ID=MsG0280010695.01.T02:exon:7277;Parent=MsG0280010695.01.T02
Chr2	five_prime_UTR	73481567	73482003	73481567	ID=MsG0280010695.01.T02:five_prime_utr;Parent=MsG0280010695.01.T02
Chr2	CDS	73482004	73482089	73482004	ID=MsG0280010695.01.T02:cds;Parent=MsG0280010695.01.T02
Chr2	CDS	73483848	73484059	73483848	ID=MsG0280010695.01.T02:cds;Parent=MsG0280010695.01.T02
Chr2	CDS	73484601	73484717	73484601	ID=MsG0280010695.01.T02:cds;Parent=MsG0280010695.01.T02
Chr2	CDS	73484807	73484976	73484807	ID=MsG0280010695.01.T02:cds;Parent=MsG0280010695.01.T02
Chr2	CDS	73485598	73485708	73485598	ID=MsG0280010695.01.T02:cds;Parent=MsG0280010695.01.T02
Chr2	three_prime_UTR	73485709	73485905	73485709	ID=MsG0280010695.01.T02:three_prime_utr;Parent=MsG0280010695.01.T02

Gene Sequence

>MsG0280010695.01.T02

ATGGGAGAGTGTTGGTCTGGTGGTGAAACGGGTACCGCTACTGCTACTATTATGATTCTTGGTGATACTTGTACTCGAGGTTGCAGATTTTGCAATGTTAAGACATCAAGGACTCCTCCACCACCTGACCCTGATGAGCCCACTAATGTGGCTGAAGCAATTGCATCGTGGGGTTTGGATTATGTGGTTATAACAAGCGTTGACCGTGATGATTTACCTGATCAAGGGAGCGGTCATTTTACTGAAACAGTTCAGAAGCTGAAGATACTGAAACCTAGCATACTGATAGAAGCCTTAGTCCCCGACTTTCGAGGAAATGCTGAGTGTGTAGAGAAGGTTTCCAAATCAGGATTAGATGTCCTTGCGCATAATATTGAGACAGTTGAAGAGCTACAGAGTGCTGTGCGGGATCACCGTGTGGGAAACCCTGACCAAATTGTGAAGACAATGGAGAAGGTGAGAGCAGCAGGTGTTGATGTGATGACATTTGGTCAGTATATGAGACCCTCCAAACGCCATATGCCAGTATCAGAATATATTACACCTGAGGCCTTTGAGAAGTATCAGACTATTGGCATGGAAATGGGATTTCGATATGTGGCATCCGGGCCCATGGTAAGGTCATCATACAAGGCAGGTGAATTCTATATTAAATCCATGATTAATTCAGATCGGGCTGTATCTTCTCAAAGCTGA

>MsG0280010695.01.T01

Protein sequence

>MsG0280010695.01.T02

MGECWSGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPDEPTNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFTETVQKLKILKPSILIEALVPDFRGNAECVEKVSKSGLDVLAHNIETVEELQSAVRDHRVGNPDQIVKTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQTIGMEMGFRYVASGPMVRSSYKAGEFYIKSMINSDRAVSSQS*

>MsG0280010695.01.T01