Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009770.01.T01 | XP_013464357.1 | 95.423 | 284 | 13 | 0 | 1 | 284 | 1 | 284 | 0 | 558 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009770.01.T01 | Q9XGI7 | 48.485 | 264 | 136 | 0 | 18 | 281 | 9 | 272 | 5.83E-92 | 278 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009770.01.T01 | A0A072V9C6 | 95.423 | 284 | 13 | 0 | 1 | 284 | 1 | 284 | 0.0 | 558 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280009432.01 | MsG0280009770.01 | 0.815415 | 9.355553e-52 | 6.445939e-49 |
| MsG0280009770.01 | MsG0380016490.01 | 0.810896 | 9.176366e-51 | 5.599750e-48 |
| MsG0280009770.01 | MsG0380017901.01 | 0.822320 | 2.523593e-53 | 2.105195e-50 |
| MsG0280009770.01 | MsG0480022840.01 | 0.808567 | 2.906922e-50 | 1.667688e-47 |
| MsG0280009770.01 | MsG0680030613.01 | 0.816387 | 5.677603e-52 | 4.017418e-49 |
| MsG0280009770.01 | MsG0780040716.01 | 0.800499 | 1.400839e-48 | 6.532653e-46 |
| MsG0280009770.01 | MsG0780040808.01 | 0.802717 | 4.914251e-49 | 2.424481e-46 |
| MsG0280009770.01 | MsG0880043129.01 | 0.801524 | 8.645640e-49 | 4.137856e-46 |
| MsG0280009770.01 | MsG0880043130.01 | 0.800475 | 1.416686e-48 | 6.602471e-46 |
| MsG0280009770.01 | MsG0880045686.01 | 0.803185 | 3.933485e-49 | 1.963861e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009770.01.T01 | MTR_2g070310 | 95.423 | 284 | 13 | 0 | 1 | 284 | 1 | 284 | 0.0 | 558 |
| MsG0280009770.01.T01 | MTR_1g084050 | 49.064 | 267 | 136 | 0 | 15 | 281 | 38 | 304 | 1.78e-92 | 277 |
| MsG0280009770.01.T01 | MTR_7g112130 | 47.566 | 267 | 140 | 0 | 15 | 281 | 5 | 271 | 5.74e-90 | 270 |
| MsG0280009770.01.T01 | MTR_2g034240 | 47.584 | 269 | 139 | 2 | 15 | 281 | 5 | 273 | 4.12e-85 | 256 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009770.01.T01 | AT5G56080 | 52.030 | 271 | 125 | 3 | 15 | 281 | 5 | 274 | 1.15e-90 | 272 |
| MsG0280009770.01.T01 | AT5G04950 | 49.815 | 271 | 132 | 2 | 15 | 281 | 5 | 275 | 2.28e-90 | 271 |
| MsG0280009770.01.T01 | AT1G56430 | 48.507 | 268 | 133 | 4 | 18 | 281 | 9 | 275 | 7.25e-88 | 265 |
| MsG0280009770.01.T01 | AT1G09240 | 51.111 | 270 | 127 | 4 | 16 | 281 | 6 | 274 | 8.86e-88 | 265 |
Find 56 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCACTAAGTTCAATTATAT+TGG | 0.247107 | 2:+57762342 | MsG0280009770.01.T01:CDS |
| ATTTGTGACACAAGATATTA+TGG | 0.267068 | 2:+57762477 | MsG0280009770.01.T01:CDS |
| TAGCTTATCGATCAAGCTTT+AGG | 0.325153 | 2:-57762760 | None:intergenic |
| TTTCTAAGCTAGAATCTCTT+AGG | 0.328740 | 2:+57762002 | MsG0280009770.01.T01:CDS |
| CCCTTCTGCTTTGCCACAAA+GGG | 0.350102 | 2:-57762143 | None:intergenic |
| CAGCTTCAAAATTGAAGGAT+TGG | 0.377323 | 2:-57761928 | None:intergenic |
| TGCTAGCAAGATTGTGGCTT+CGG | 0.391793 | 2:+57762429 | MsG0280009770.01.T01:CDS |
| CATTCACAACACCATTCTCT+TGG | 0.394956 | 2:-57762285 | None:intergenic |
| TTAACATAGTTTCCATGGTA+AGG | 0.396731 | 2:-57762235 | None:intergenic |
| GCCAATATAATTGAACTTAG+TGG | 0.399358 | 2:-57762343 | None:intergenic |
| ATGGAAGCTAAGGAGAGGTT+AGG | 0.399895 | 2:+57762496 | MsG0280009770.01.T01:CDS |
| AAAGTAGCATTTATAGGATC+TGG | 0.401897 | 2:+57762313 | MsG0280009770.01.T01:CDS |
| ATATCATTCTCCTCAACAAT+AGG | 0.407208 | 2:-57762664 | None:intergenic |
| GAAAGCCTCATTCCCCTTTG+TGG | 0.425154 | 2:+57762130 | MsG0280009770.01.T01:CDS |
| AAGAGATTCTAGCTTAGAAA+TGG | 0.430571 | 2:-57761999 | None:intergenic |
| CGCATTGTCTTAACCTCTTG+TGG | 0.432545 | 2:-57762106 | None:intergenic |
| TTGCTTGTAAGAAGTGCAAA+AGG | 0.435002 | 2:+57762622 | MsG0280009770.01.T01:CDS |
| ATCTCTTAGGCCTTCAAAGC+AGG | 0.436005 | 2:+57762015 | MsG0280009770.01.T01:CDS |
| AGGGTGTTAACCTGCTTTGA+AGG | 0.438492 | 2:-57762025 | None:intergenic |
| ATAATTGAACTTAGTGGCAT+TGG | 0.438717 | 2:-57762337 | None:intergenic |
| TGTCTTAACCTCTTGTGGTA+AGG | 0.449698 | 2:-57762101 | None:intergenic |
| TATTGTTGAGGAGAATGATA+TGG | 0.461887 | 2:+57762666 | MsG0280009770.01.T01:CDS |
| GAGATATGAGAAGCTCTTGT+GGG | 0.466406 | 2:-57761955 | None:intergenic |
| GTTTGACTAAGTGAGTCAAA+AGG | 0.470350 | 2:-57762045 | None:intergenic |
| GATTTCAGCTTCAAAATTGA+AGG | 0.471295 | 2:-57761933 | None:intergenic |
| CTTTGCCACAAAGGGGAATG+AGG | 0.471873 | 2:-57762135 | None:intergenic |
| CTAGTTTAACATAGTTTCCA+TGG | 0.475050 | 2:-57762240 | None:intergenic |
| AGCAAAATCCTCCAAGAGAA+TGG | 0.476745 | 2:+57762274 | MsG0280009770.01.T01:CDS |
| CCCCTTTGTGGCAAAGCAGA+AGG | 0.479250 | 2:+57762142 | MsG0280009770.01.T01:CDS |
| GCCCTTCTGCTTTGCCACAA+AGG | 0.484713 | 2:-57762144 | None:intergenic |
| TCACAACACCATTCTCTTGG+AGG | 0.492086 | 2:-57762282 | None:intergenic |
| TAACATAGTTTCCATGGTAA+GGG | 0.496507 | 2:-57762234 | None:intergenic |
| TGGTAAGGCTTCAATCTCAA+TGG | 0.508290 | 2:-57762086 | None:intergenic |
| GCTTCAATCTCAATGGATGA+AGG | 0.509882 | 2:-57762079 | None:intergenic |
| ACAAGATATTATGGAAGCTA+AGG | 0.511704 | 2:+57762486 | MsG0280009770.01.T01:CDS |
| AAGGATTGGAAAGATGCCAT+TGG | 0.518413 | 2:-57761914 | None:intergenic |
| CGGATAAAGCACTTGAAAAG+AGG | 0.520656 | 2:+57762449 | MsG0280009770.01.T01:CDS |
| TTTGACTAAGTGAGTCAAAA+GGG | 0.523641 | 2:-57762044 | None:intergenic |
| TGGAAGCTAAGGAGAGGTTA+GGG | 0.528228 | 2:+57762497 | MsG0280009770.01.T01:CDS |
| GATTGAAGCCTTACCACAAG+AGG | 0.528285 | 2:+57762093 | MsG0280009770.01.T01:CDS |
| CCCTTTGTGGCAAAGCAGAA+GGG | 0.545668 | 2:+57762143 | MsG0280009770.01.T01:CDS |
| TGAGATATGAGAAGCTCTTG+TGG | 0.550637 | 2:-57761956 | None:intergenic |
| ATAAGAAAGTATATGAAGGA+AGG | 0.551950 | 2:+57762592 | MsG0280009770.01.T01:CDS |
| TGCTTGTAAGAAGTGCAAAA+GGG | 0.561089 | 2:+57762623 | MsG0280009770.01.T01:CDS |
| TGAGGTTGCTAGCAAGATTG+TGG | 0.575623 | 2:+57762423 | MsG0280009770.01.T01:CDS |
| ACATATAAGAAAGTATATGA+AGG | 0.575751 | 2:+57762588 | MsG0280009770.01.T01:CDS |
| TATTGATGAGAAAGCTAATG+AGG | 0.589845 | 2:+57762405 | MsG0280009770.01.T01:CDS |
| CTTCAATCTCAATGGATGAA+GGG | 0.591587 | 2:-57762078 | None:intergenic |
| AGAAAGTATATGAAGGAAGG+TGG | 0.592605 | 2:+57762595 | MsG0280009770.01.T01:CDS |
| TATATTGGCAACTGATCACA+TGG | 0.597728 | 2:+57762357 | MsG0280009770.01.T01:CDS |
| AACGGATGATGTTATCAACT+CGG | 0.627733 | 2:+57762720 | MsG0280009770.01.T01:CDS |
| CTGCACTTGTTGGTATGCGT+AGG | 0.635388 | 2:+57762542 | MsG0280009770.01.T01:CDS |
| CCTTCTGCTTTGCCACAAAG+GGG | 0.652298 | 2:-57762142 | None:intergenic |
| CAATCTCAATGGATGAAGGG+AGG | 0.659476 | 2:-57762075 | None:intergenic |
| GAGTTGATAACATCATCCGT+TGG | 0.686377 | 2:-57762718 | None:intergenic |
| ATATTATGGAAGCTAAGGAG+AGG | 0.694503 | 2:+57762491 | MsG0280009770.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAAGTGTTAAATTTTTCAC+TGG | - | Chr2:57762214-57762233 | None:intergenic | 20.0% |
| !!! | ACATATAAGAAAGTATATGA+AGG | + | Chr2:57762588-57762607 | MsG0280009770.01.T01:CDS | 20.0% |
| ! | ATTTGTGACACAAGATATTA+TGG | + | Chr2:57762477-57762496 | MsG0280009770.01.T01:CDS | 25.0% |
| ! | GCAAAAAAAGTAGCATTTAT+AGG | + | Chr2:57762307-57762326 | MsG0280009770.01.T01:CDS | 25.0% |
| !! | AGTGAAAAAGTGAAGATTTT+GGG | + | Chr2:57762565-57762584 | MsG0280009770.01.T01:CDS | 25.0% |
| !! | ATAAGAAAGTATATGAAGGA+AGG | + | Chr2:57762592-57762611 | MsG0280009770.01.T01:CDS | 25.0% |
| !! | GTTAAATTTTTCACTGGATT+TGG | - | Chr2:57762208-57762227 | None:intergenic | 25.0% |
| !!! | GCAATATGATTGCATTTTTT+TGG | + | Chr2:57762519-57762538 | MsG0280009770.01.T01:CDS | 25.0% |
| !!! | TTTTTTGTATCCTATTGTTG+AGG | + | Chr2:57762654-57762673 | MsG0280009770.01.T01:CDS | 25.0% |
| AAAGTAGCATTTATAGGATC+TGG | + | Chr2:57762313-57762332 | MsG0280009770.01.T01:CDS | 30.0% | |
| ACAAGATATTATGGAAGCTA+AGG | + | Chr2:57762486-57762505 | MsG0280009770.01.T01:CDS | 30.0% | |
| ATAATTGAACTTAGTGGCAT+TGG | - | Chr2:57762340-57762359 | None:intergenic | 30.0% | |
| ATATCATTCTCCTCAACAAT+AGG | - | Chr2:57762667-57762686 | None:intergenic | 30.0% | |
| CTAGTTTAACATAGTTTCCA+TGG | - | Chr2:57762243-57762262 | None:intergenic | 30.0% | |
| GATTTCAGCTTCAAAATTGA+AGG | - | Chr2:57761936-57761955 | None:intergenic | 30.0% | |
| GCCAATATAATTGAACTTAG+TGG | - | Chr2:57762346-57762365 | None:intergenic | 30.0% | |
| GCCACTAAGTTCAATTATAT+TGG | + | Chr2:57762342-57762361 | MsG0280009770.01.T01:CDS | 30.0% | |
| TAACATAGTTTCCATGGTAA+GGG | - | Chr2:57762237-57762256 | None:intergenic | 30.0% | |
| TATTGATGAGAAAGCTAATG+AGG | + | Chr2:57762405-57762424 | MsG0280009770.01.T01:CDS | 30.0% | |
| TATTGTTGAGGAGAATGATA+TGG | + | Chr2:57762666-57762685 | MsG0280009770.01.T01:CDS | 30.0% | |
| TTAACATAGTTTCCATGGTA+AGG | - | Chr2:57762238-57762257 | None:intergenic | 30.0% | |
| TTTCTAAGCTAGAATCTCTT+AGG | + | Chr2:57762002-57762021 | MsG0280009770.01.T01:CDS | 30.0% | |
| TTTGACTAAGTGAGTCAAAA+GGG | - | Chr2:57762047-57762066 | None:intergenic | 30.0% | |
| ! | GAGTGAAAAAGTGAAGATTT+TGG | + | Chr2:57762564-57762583 | MsG0280009770.01.T01:CDS | 30.0% |
| ! | GCTTACAATTTTTCATCCAA+CGG | + | Chr2:57762702-57762721 | MsG0280009770.01.T01:CDS | 30.0% |
| !! | AAGAGATTCTAGCTTAGAAA+TGG | - | Chr2:57762002-57762021 | None:intergenic | 30.0% |
| AACGGATGATGTTATCAACT+CGG | + | Chr2:57762720-57762739 | MsG0280009770.01.T01:CDS | 35.0% | |
| ATATTATGGAAGCTAAGGAG+AGG | + | Chr2:57762491-57762510 | MsG0280009770.01.T01:CDS | 35.0% | |
| CAGCTTCAAAATTGAAGGAT+TGG | - | Chr2:57761931-57761950 | None:intergenic | 35.0% | |
| CTTCAATCTCAATGGATGAA+GGG | - | Chr2:57762081-57762100 | None:intergenic | 35.0% | |
| GTTTGACTAAGTGAGTCAAA+AGG | - | Chr2:57762048-57762067 | None:intergenic | 35.0% | |
| TATATTGGCAACTGATCACA+TGG | + | Chr2:57762357-57762376 | MsG0280009770.01.T01:CDS | 35.0% | |
| ! | AGAAAGTATATGAAGGAAGG+TGG | + | Chr2:57762595-57762614 | MsG0280009770.01.T01:CDS | 35.0% |
| ! | TGCTTGTAAGAAGTGCAAAA+GGG | + | Chr2:57762623-57762642 | MsG0280009770.01.T01:CDS | 35.0% |
| ! | TTAACACTTTTCCCTTACCA+TGG | + | Chr2:57762223-57762242 | MsG0280009770.01.T01:CDS | 35.0% |
| ! | TTGCTTGTAAGAAGTGCAAA+AGG | + | Chr2:57762622-57762641 | MsG0280009770.01.T01:CDS | 35.0% |
| !!! | ATTTTTTTGGCTGCACTTGT+TGG | + | Chr2:57762532-57762551 | MsG0280009770.01.T01:CDS | 35.0% |
| !!! | TTTTCACTGGATTTGGTGTT+TGG | - | Chr2:57762201-57762220 | None:intergenic | 35.0% |
| AGCAAAATCCTCCAAGAGAA+TGG | + | Chr2:57762274-57762293 | MsG0280009770.01.T01:CDS | 40.0% | |
| CATTCACAACACCATTCTCT+TGG | - | Chr2:57762288-57762307 | None:intergenic | 40.0% | |
| GAGATATGAGAAGCTCTTGT+GGG | - | Chr2:57761958-57761977 | None:intergenic | 40.0% | |
| GCTTCAATCTCAATGGATGA+AGG | - | Chr2:57762082-57762101 | None:intergenic | 40.0% | |
| TGAGATATGAGAAGCTCTTG+TGG | - | Chr2:57761959-57761978 | None:intergenic | 40.0% | |
| TGGTAAGGCTTCAATCTCAA+TGG | - | Chr2:57762089-57762108 | None:intergenic | 40.0% | |
| TGTCTTAACCTCTTGTGGTA+AGG | - | Chr2:57762104-57762123 | None:intergenic | 40.0% | |
| ! | CGGATAAAGCACTTGAAAAG+AGG | + | Chr2:57762449-57762468 | MsG0280009770.01.T01:CDS | 40.0% |
| !! | GAGTTGATAACATCATCCGT+TGG | - | Chr2:57762721-57762740 | None:intergenic | 40.0% |
| ATCTCTTAGGCCTTCAAAGC+AGG | + | Chr2:57762015-57762034 | MsG0280009770.01.T01:CDS | 45.0% | |
| ATGGAAGCTAAGGAGAGGTT+AGG | + | Chr2:57762496-57762515 | MsG0280009770.01.T01:CDS | 45.0% | |
| CAATCTCAATGGATGAAGGG+AGG | - | Chr2:57762078-57762097 | None:intergenic | 45.0% | |
| CGCATTGTCTTAACCTCTTG+TGG | - | Chr2:57762109-57762128 | None:intergenic | 45.0% | |
| GATTGAAGCCTTACCACAAG+AGG | + | Chr2:57762093-57762112 | MsG0280009770.01.T01:CDS | 45.0% | |
| TCACAACACCATTCTCTTGG+AGG | - | Chr2:57762285-57762304 | None:intergenic | 45.0% | |
| TGAGGTTGCTAGCAAGATTG+TGG | + | Chr2:57762423-57762442 | MsG0280009770.01.T01:CDS | 45.0% | |
| TGCTAGCAAGATTGTGGCTT+CGG | + | Chr2:57762429-57762448 | MsG0280009770.01.T01:CDS | 45.0% | |
| TGGAAGCTAAGGAGAGGTTA+GGG | + | Chr2:57762497-57762516 | MsG0280009770.01.T01:CDS | 45.0% | |
| ! | AGGGTGTTAACCTGCTTTGA+AGG | - | Chr2:57762028-57762047 | None:intergenic | 45.0% |
| CCCTTCTGCTTTGCCACAAA+GGG | - | Chr2:57762146-57762165 | None:intergenic | 50.0% | |
| CCCTTTGTGGCAAAGCAGAA+GGG | + | Chr2:57762143-57762162 | MsG0280009770.01.T01:CDS | 50.0% | |
| CCTTCTGCTTTGCCACAAAG+GGG | - | Chr2:57762145-57762164 | None:intergenic | 50.0% | |
| CTTTGCCACAAAGGGGAATG+AGG | - | Chr2:57762138-57762157 | None:intergenic | 50.0% | |
| GAAAGCCTCATTCCCCTTTG+TGG | + | Chr2:57762130-57762149 | MsG0280009770.01.T01:CDS | 50.0% | |
| ! | CTGCACTTGTTGGTATGCGT+AGG | + | Chr2:57762542-57762561 | MsG0280009770.01.T01:CDS | 50.0% |
| CCCCTTTGTGGCAAAGCAGA+AGG | + | Chr2:57762142-57762161 | MsG0280009770.01.T01:CDS | 55.0% | |
| GCCCTTCTGCTTTGCCACAA+AGG | - | Chr2:57762147-57762166 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 57761917 | 57762771 | 57761917 | ID=MsG0280009770.01;Name=MsG0280009770.01 |
| Chr2 | mRNA | 57761917 | 57762771 | 57761917 | ID=MsG0280009770.01.T01;Parent=MsG0280009770.01;Name=MsG0280009770.01.T01;_AED=0.10;_eAED=0.10;_QI=0|-1|0|1|-1|1|1|0|284 |
| Chr2 | exon | 57761917 | 57762771 | 57761917 | ID=MsG0280009770.01.T01:exon:16462;Parent=MsG0280009770.01.T01 |
| Chr2 | CDS | 57761917 | 57762771 | 57761917 | ID=MsG0280009770.01.T01:cds;Parent=MsG0280009770.01.T01 |
| Gene Sequence |
| Protein sequence |
