AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016490.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016490.01.T01 MTR_3g090830 98.413 63 1 0 1 63 1 63 4.11e-38 120
MsG0380016490.01.T01 MTR_1g028610 76.271 59 12 1 7 63 4 62 2.39e-18 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016490.01.T01 AT2G46330 63.462 52 19 0 12 63 11 62 1.02e-18 72.4
MsG0380016490.01.T01 AT3G61640 56.667 60 26 0 4 63 3 62 1.41e-17 69.3
MsG0380016490.01.T01 AT5G53250 58.182 55 23 0 9 63 9 63 2.32e-15 63.5
MsG0380016490.01.T01 AT5G24105 63.492 63 23 0 1 63 1 63 5.02e-11 52.4

Find 38 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACAAGTCTTGTAATTATT+TGG 0.106778 3:-84187595 MsG0380016490.01.T01:five_prime_UTR
CTGTTTCATGTGCTTCATTC+AGG 0.175879 3:-84187443 MsG0380016490.01.T01:CDS
CTCTCTTTGCCTTTAAAATT+AGG 0.193691 3:+84186502 None:intergenic
ATCTATAGATTATTGTGTTC+TGG 0.249557 3:-84186371 MsG0380016490.01.T01:three_prime_UTR
TGTTCTAATGCTTGTGGCTT+TGG 0.292333 3:-84186605 MsG0380016490.01.T01:CDS
TTCAGGGTGGTTGCCTTCCT+TGG 0.296499 3:-84187426 MsG0380016490.01.T01:CDS
TGTGTAGGAGAAAGATGTTC+GGG 0.392777 3:+84187613 None:intergenic
GATAAATTAATGAATGATAT+AGG 0.396044 3:+84186576 None:intergenic
AATTAAAATGAAATTCTCAT+TGG 0.396792 3:+84186403 None:intergenic
AATATTAAGAGAAACATATA+TGG 0.399652 3:-84187466 MsG0380016490.01.T01:exon
GAATATTTACCATCGCTTGT+AGG 0.408971 3:+84187342 None:intergenic
TGTTTCATGTGCTTCATTCA+GGG 0.448362 3:-84187442 MsG0380016490.01.T01:CDS
TTACCATCGCTTGTAGGAGC+AGG 0.455603 3:+84187348 None:intergenic
CTAAATGAGGAGTTTCTCTC+TGG 0.478944 3:+84186551 None:intergenic
CGTGAATTTAGAACTAAATG+AGG 0.493233 3:+84186538 None:intergenic
TTGTGTAGGAGAAAGATGTT+CGG 0.508853 3:+84187612 None:intergenic
TGAAATTCTCATTGGAGCAT+AGG 0.513938 3:+84186411 None:intergenic
AATAATTACAAGACTTGTGT+AGG 0.529714 3:+84187598 None:intergenic
AGGAGCAGGGGCAGGTGCAA+TGG 0.532358 3:+84187362 None:intergenic
TTATGCAACACTTATTTCTG+TGG 0.538882 3:-84187397 MsG0380016490.01.T01:CDS
TACCATCGCTTGTAGGAGCA+GGG 0.542274 3:+84187349 None:intergenic
AATGAGGAGTTTCTCTCTGG+TGG 0.547747 3:+84186554 None:intergenic
AAATAAGTGTTGCATAAATG+AGG 0.548157 3:+84187403 None:intergenic
TGCATGTGTTCTAATGCTTG+TGG 0.551000 3:-84186611 MsG0380016490.01.T01:CDS
TTTAGTTCTAAATTCACGAA+TGG 0.560942 3:-84186533 MsG0380016490.01.T01:three_prime_UTR
TCGCTTGTAGGAGCAGGGGC+AGG 0.566771 3:+84187354 None:intergenic
TGCATAAATGAGGCCAAGGA+AGG 0.567934 3:+84187413 None:intergenic
TTAGAACACATGCAATCGCT+TGG 0.574241 3:+84186620 None:intergenic
AAAATCAAGAGAGAAAGAGA+AGG 0.577239 3:-84187525 MsG0380016490.01.T01:five_prime_UTR
TGCAATCGCTTGGTCAATTG+TGG 0.581446 3:+84186630 None:intergenic
GTGTTGCATAAATGAGGCCA+AGG 0.582795 3:+84187409 None:intergenic
GCCCCTGCTCCTACAAGCGA+TGG 0.595947 3:-84187351 MsG0380016490.01.T01:intron
ACCATCGCTTGTAGGAGCAG+GGG 0.636251 3:+84187350 None:intergenic
TTCATGTCATAACAGAGAGG+AGG 0.646835 3:-84186472 MsG0380016490.01.T01:three_prime_UTR
TTCATGTGCTTCATTCAGGG+TGG 0.668781 3:-84187439 MsG0380016490.01.T01:CDS
TCATTCATGTCATAACAGAG+AGG 0.730488 3:-84186475 MsG0380016490.01.T01:three_prime_UTR
TAGGAGAAAGATGTTCGGGG+TGG 0.741469 3:+84187617 None:intergenic
GTGTAGGAGAAAGATGTTCG+GGG 0.753463 3:+84187614 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGAATTTCATTTTAATTTAT+TGG - Chr3:84187561-84187580 MsG0380016490.01.T01:five_prime_UTR 10.0%
!! AACAAATTGAAAATCAAATA+TGG + Chr3:84187216-84187235 None:intergenic 15.0%
!! AATATTAAGAGAAACATATA+TGG - Chr3:84186492-84186511 MsG0380016490.01.T01:three_prime_UTR 15.0%
!! AATTAAAATGAAATTCTCAT+TGG + Chr3:84187558-84187577 None:intergenic 15.0%
!! GATAAATTAATGAATGATAT+AGG + Chr3:84187385-84187404 None:intergenic 15.0%
!! TAATGATTATAAAACAAATG+TGG - Chr3:84187051-84187070 MsG0380016490.01.T01:intron 15.0%
!! TGAAAAATAAATTAAAATGG+AGG + Chr3:84186732-84186751 None:intergenic 15.0%
!!! AATCATTTCATAAATCTTTT+TGG - Chr3:84186926-84186945 MsG0380016490.01.T01:intron 15.0%
!!! TTTATTTTCAGAGATTAAAT+TGG + Chr3:84186772-84186791 None:intergenic 15.0%
!! AAAGTGATGAAACATTAATA+AGG + Chr3:84186672-84186691 None:intergenic 20.0%
!! AAGTGATGAAACATTAATAA+GGG + Chr3:84186671-84186690 None:intergenic 20.0%
!! GCTAAAAGTTAAGTAAAATT+TGG + Chr3:84187133-84187152 None:intergenic 20.0%
!!! AAAACACCTTATACTTTTTA+AGG - Chr3:84186968-84186987 MsG0380016490.01.T01:intron 20.0%
!!! AATTGTTGTTTATAATATGC+AGG - Chr3:84187303-84187322 MsG0380016490.01.T01:intron 20.0%
!!! ATTTAGCCTTAAAAAGTATA+AGG + Chr3:84186977-84186996 None:intergenic 20.0%
! AATAATTACAAGACTTGTGT+AGG + Chr3:84186363-84186382 None:intergenic 25.0%
! ATCTATAGATTATTGTGTTC+TGG - Chr3:84187587-84187606 MsG0380016490.01.T01:five_prime_UTR 25.0%
! TAATATCCATATAAACGGTT+AGG - Chr3:84187076-84187095 MsG0380016490.01.T01:intron 25.0%
! TCTTTATGAGCATTATTGAT+AGG - Chr3:84187254-84187273 MsG0380016490.01.T01:intron 25.0%
! TGGACTAATATCCATATAAA+CGG - Chr3:84187071-84187090 MsG0380016490.01.T01:intron 25.0%
!! AAATAAGTGTTGCATAAATG+AGG + Chr3:84186558-84186577 None:intergenic 25.0%
!! ACACAAGTCTTGTAATTATT+TGG - Chr3:84186363-84186382 MsG0380016490.01.T01:three_prime_UTR 25.0%
!! ATCAAATATGGAGATGTTTT+TGG + Chr3:84187204-84187223 None:intergenic 25.0%
!! GATTAAATTGGTGATTCATT+TGG + Chr3:84186760-84186779 None:intergenic 25.0%
!!! AAACTCACATATATTTTGAG+AGG + Chr3:84187009-84187028 None:intergenic 25.0%
!!! AAGTTTTTAGAGAGAAAGAA+AGG + Chr3:84186411-84186430 None:intergenic 25.0%
!!! CTTTCAATCACATTAGTTTT+CGG + Chr3:84186897-84186916 None:intergenic 25.0%
!!! TTTAGTTCTAAATTCACGAA+TGG - Chr3:84187425-84187444 MsG0380016490.01.T01:CDS 25.0%
AAAATCAAGAGAGAAAGAGA+AGG - Chr3:84186433-84186452 MsG0380016490.01.T01:three_prime_UTR 30.0%
GCTCATAAAGAATAATAGCA+TGG + Chr3:84187245-84187264 None:intergenic 30.0%
! CGTGAATTTAGAACTAAATG+AGG + Chr3:84187423-84187442 None:intergenic 30.0%
!! CTAATTTTAAAGGCAAAGAG+AGG - Chr3:84187457-84187476 MsG0380016490.01.T01:exon 30.0%
!! CTCTCTTTGCCTTTAAAATT+AGG + Chr3:84187459-84187478 None:intergenic 30.0%
!! TTATGCAACACTTATTTCTG+TGG - Chr3:84186561-84186580 MsG0380016490.01.T01:three_prime_UTR 30.0%
GAATATTTACCATCGCTTGT+AGG + Chr3:84186619-84186638 None:intergenic 35.0%
GTTCTTCCTAACCGTTTATA+TGG + Chr3:84187085-84187104 None:intergenic 35.0%
TCATTCATGTCATAACAGAG+AGG - Chr3:84187483-84187502 MsG0380016490.01.T01:five_prime_UTR 35.0%
TGAAATTCTCATTGGAGCAT+AGG + Chr3:84187550-84187569 None:intergenic 35.0%
TGTTTCATGTGCTTCATTCA+GGG - Chr3:84186516-84186535 MsG0380016490.01.T01:three_prime_UTR 35.0%
TTGTGTAGGAGAAAGATGTT+CGG + Chr3:84186349-84186368 None:intergenic 35.0%
! GCTACAGAGCCTAATTTTAA+AGG - Chr3:84187447-84187466 MsG0380016490.01.T01:CDS 35.0%
!! GAGGAGGATGAGATTTTAAT+TGG - Chr3:84187502-84187521 MsG0380016490.01.T01:five_prime_UTR 35.0%
AAGATTGTTCCTACAAACCC+AGG + Chr3:84187107-84187126 None:intergenic 40.0%
ATAAACGGTTAGGAAGAACC+TGG - Chr3:84187086-84187105 MsG0380016490.01.T01:intron 40.0%
CTAAATGAGGAGTTTCTCTC+TGG + Chr3:84187410-84187429 None:intergenic 40.0%
CTGTTTCATGTGCTTCATTC+AGG - Chr3:84186515-84186534 MsG0380016490.01.T01:three_prime_UTR 40.0%
TAAACGGTTAGGAAGAACCT+GGG - Chr3:84187087-84187106 MsG0380016490.01.T01:intron 40.0%
TCCCATTCCCTAGAACTAAA+GGG - Chr3:84187172-84187191 MsG0380016490.01.T01:intron 40.0%
TGCATGTGTTCTAATGCTTG+TGG - Chr3:84187347-84187366 MsG0380016490.01.T01:intron 40.0%
TGTGTAGGAGAAAGATGTTC+GGG + Chr3:84186348-84186367 None:intergenic 40.0%
TGTTCTAATGCTTGTGGCTT+TGG - Chr3:84187353-84187372 MsG0380016490.01.T01:CDS 40.0%
TTAGAACACATGCAATCGCT+TGG + Chr3:84187341-84187360 None:intergenic 40.0%
TTCATGTCATAACAGAGAGG+AGG - Chr3:84187486-84187505 MsG0380016490.01.T01:five_prime_UTR 40.0%
!! AACCCTTTAGTTCTAGGGAA+TGG + Chr3:84187177-84187196 None:intergenic 40.0%
!! ACCCTTTAGTTCTAGGGAAT+GGG + Chr3:84187176-84187195 None:intergenic 40.0%
AATGAGGAGTTTCTCTCTGG+TGG + Chr3:84187407-84187426 None:intergenic 45.0%
GTCCCATTCCCTAGAACTAA+AGG - Chr3:84187171-84187190 MsG0380016490.01.T01:intron 45.0%
GTGTAGGAGAAAGATGTTCG+GGG + Chr3:84186347-84186366 None:intergenic 45.0%
GTGTTGCATAAATGAGGCCA+AGG + Chr3:84186552-84186571 None:intergenic 45.0%
TAGGAAGAACCTGGGTTTGT+AGG - Chr3:84187095-84187114 MsG0380016490.01.T01:intron 45.0%
TCCCTAGAACTAAAGGGTTG+TGG - Chr3:84187178-84187197 MsG0380016490.01.T01:intron 45.0%
TGCAATCGCTTGGTCAATTG+TGG + Chr3:84187331-84187350 None:intergenic 45.0%
TGCATAAATGAGGCCAAGGA+AGG + Chr3:84186548-84186567 None:intergenic 45.0%
TTCATGTGCTTCATTCAGGG+TGG - Chr3:84186519-84186538 MsG0380016490.01.T01:three_prime_UTR 45.0%
!! GCCACAACCCTTTAGTTCTA+GGG + Chr3:84187182-84187201 None:intergenic 45.0%
!! TAGTTCTAGGGAATGGGACA+TGG + Chr3:84187170-84187189 None:intergenic 45.0%
!! TATTGAAAAATAAATTAAAA+TGG + Chr3:84186735-84186754 None:intergenic 5.0%
TACCATCGCTTGTAGGAGCA+GGG + Chr3:84186612-84186631 None:intergenic 50.0%
TAGGAGAAAGATGTTCGGGG+TGG + Chr3:84186344-84186363 None:intergenic 50.0%
TTACCATCGCTTGTAGGAGC+AGG + Chr3:84186613-84186632 None:intergenic 50.0%
!! GGCCACAACCCTTTAGTTCT+AGG + Chr3:84187183-84187202 None:intergenic 50.0%
ACCATCGCTTGTAGGAGCAG+GGG + Chr3:84186611-84186630 None:intergenic 55.0%
TTCAGGGTGGTTGCCTTCCT+TGG - Chr3:84186532-84186551 MsG0380016490.01.T01:three_prime_UTR 55.0%
AGGAGCAGGGGCAGGTGCAA+TGG + Chr3:84186599-84186618 None:intergenic 65.0%
GCCCCTGCTCCTACAAGCGA+TGG - Chr3:84186607-84186626 MsG0380016490.01.T01:CDS 65.0%
TCGCTTGTAGGAGCAGGGGC+AGG + Chr3:84186607-84186626 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr3 gene 84186340 84187640 84186340 ID=MsG0380016490.01;Name=MsG0380016490.01
Chr3 mRNA 84186340 84187640 84186340 ID=MsG0380016490.01.T01;Parent=MsG0380016490.01;Name=MsG0380016490.01.T01;_AED=0.49;_eAED=0.49;_QI=171|1|1|1|0|0|2|242|63
Chr3 exon 84186340 84186655 84186340 ID=MsG0380016490.01.T01:exon:33405;Parent=MsG0380016490.01.T01
Chr3 exon 84187352 84187640 84187352 ID=MsG0380016490.01.T01:exon:33404;Parent=MsG0380016490.01.T01
Chr3 five_prime_UTR 84187470 84187640 84187470 ID=MsG0380016490.01.T01:five_prime_utr;Parent=MsG0380016490.01.T01
Chr3 CDS 84187352 84187469 84187352 ID=MsG0380016490.01.T01:cds;Parent=MsG0380016490.01.T01
Chr3 CDS 84186582 84186655 84186582 ID=MsG0380016490.01.T01:cds;Parent=MsG0380016490.01.T01
Chr3 three_prime_UTR 84186340 84186581 84186340 ID=MsG0380016490.01.T01:three_prime_utr;Parent=MsG0380016490.01.T01
Gene Sequence

>MsG0380016490.01.T01

ATGGCTGTTTCATGTGCTTCATTCAGGGTGGTTGCCTTCCTTGGCCTCATTTATGCAACACTTATTTCTGTGGCTTGTTCACAATCCATTGCACCTGCCCCTGCTCCTACAAGCGATGGCACCACAATTGACCAAGCGATTGCATGTGTTCTAATGCTTGTGGCTTTGGTGTTGACCTATATCATTCATTAA

Protein sequence

>MsG0380016490.01.T01

MAVSCASFRVVAFLGLIYATLISVACSQSIAPAPAPTSDGTTIDQAIACVLMLVALVLTYIIH*