AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010218.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010218.01.T01 MTR_2g079300 82.225 782 69 8 1 715 137 915 0.0 1174
MsG0280010218.01.T01 MTR_2g079310 66.667 582 157 14 143 710 375 933 0.0 707
MsG0280010218.01.T01 MTR_2g079310 63.212 193 61 4 1 186 170 359 5.67e-55 203
MsG0280010218.01.T01 MTR_2g013640 39.216 765 377 17 16 704 178 930 2.30e-161 490
MsG0280010218.01.T01 MTR_4g011610 30.361 415 248 14 297 685 612 1011 2.64e-40 159
MsG0280010218.01.T01 MTR_4g071100 80.769 52 10 0 422 473 1 52 9.16e-23 95.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010218.01.T01 AT4G21700 39.898 589 303 16 157 714 392 960 5.71e-130 409
MsG0280010218.01.T01 AT1G52780 30.900 411 241 16 297 682 618 1010 2.17e-43 169

Find 125 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTCTTGTTGCTCCAAATT+TGG 0.143102 2:-66324038 None:intergenic
GGAAACATCATTAATGAAAT+TGG 0.171707 2:-66323476 None:intergenic
AAAAGATAGGAGCTATATTA+AGG 0.225281 2:+66324635 MsG0280010218.01.T01:CDS
AAGTAAAGAGGATCGGATTC+TGG 0.226096 2:-66324678 None:intergenic
GTAGCATAGTTGGCCAAATT+TGG 0.228475 2:+66324025 MsG0280010218.01.T01:CDS
TTGGAACTTCCACTAATTAT+AGG 0.235588 2:-66323695 None:intergenic
CTATAACTATAATTTGCTTT+TGG 0.237802 2:-66323497 None:intergenic
TCTACTTCTCCTTCTGATTC+AGG 0.242557 2:-66325617 None:intergenic
CACATGCTTACGATCTTTAC+AGG 0.250812 2:+66325396 MsG0280010218.01.T01:CDS
TCTTTACTTCAAACGTTTAG+AGG 0.271104 2:+66324692 MsG0280010218.01.T01:CDS
AGCAAATTATAGTTATAGTT+TGG 0.277090 2:+66323502 MsG0280010218.01.T01:CDS
GTTTAACATGTCTTATTATT+CGG 0.292650 2:+66324464 MsG0280010218.01.T01:CDS
AAAACATAGGTTGTGTTGTT+AGG 0.295192 2:-66324954 None:intergenic
GAGATGAAGGGAAGCACATT+AGG 0.300140 2:-66324852 None:intergenic
CTGAATGAATCAAAGTTTAA+TGG 0.303797 2:-66323587 None:intergenic
TTCAAGTAATATAACTAGTT+TGG 0.312869 2:+66323394 MsG0280010218.01.T01:CDS
AACACCAACAGCAAATTCTT+TGG 0.329524 2:+66324569 MsG0280010218.01.T01:CDS
GTTGAAAACGAGTTCTCTTT+TGG 0.330319 2:+66323536 MsG0280010218.01.T01:CDS
AGATTGAGATTCCCTCAATT+TGG 0.335511 2:+66323986 MsG0280010218.01.T01:CDS
CATAAAATCTTATGGTGGTT+TGG 0.357544 2:+66325262 MsG0280010218.01.T01:CDS
ATCTTATGGTGGTTTGGTAT+TGG 0.370141 2:+66325268 MsG0280010218.01.T01:CDS
CTTCTTTAGATACAAAAGAT+AGG 0.374932 2:+66324622 MsG0280010218.01.T01:CDS
AAGAAGAGAGAAGAAATGTT+TGG 0.375232 2:+66324739 MsG0280010218.01.T01:CDS
TCAGGGAATGTTTGCATGGT+TGG 0.376527 2:+66323296 None:intergenic
TTGGCTATATTCATACCTTA+AGG 0.379083 2:-66324133 None:intergenic
TTATTATTCGGTGAAGATTT+CGG 0.381979 2:+66324476 MsG0280010218.01.T01:CDS
AGGAATGATGATGTGAATTA+TGG 0.394823 2:+66324096 MsG0280010218.01.T01:CDS
TGGGAGAACATAAAATCTTA+TGG 0.395445 2:+66325254 MsG0280010218.01.T01:CDS
AACAAGGAATTCTTGTCAAA+AGG 0.413713 2:-66324444 None:intergenic
GAGAAGAAATGTTTGGAGAA+TGG 0.414023 2:+66324746 MsG0280010218.01.T01:CDS
AATTGCACTCCTATAATTAG+TGG 0.415041 2:+66323686 MsG0280010218.01.T01:CDS
ATGATGTCGATTCTCACGTT+TGG 0.417652 2:+66324882 MsG0280010218.01.T01:CDS
AATATAACTAGTTTGGTTAG+TGG 0.417955 2:+66323401 MsG0280010218.01.T01:CDS
AAAGATAGGAGCTATATTAA+GGG 0.423119 2:+66324636 MsG0280010218.01.T01:CDS
TTGTATCTAAAGAAGGGAAC+TGG 0.426027 2:-66324614 None:intergenic
AGATGAAGGGAAGCACATTA+GGG 0.428757 2:-66324851 None:intergenic
AAGTTAATGAAATAAGTGTT+AGG 0.433944 2:+66325000 MsG0280010218.01.T01:CDS
CATCATGAAAATTGAGATGA+AGG 0.435480 2:-66324865 None:intergenic
ATGTCATATGAATATGCATC+TGG 0.440507 2:-66324219 None:intergenic
TTCAACATTTCCTAAAACAT+AGG 0.445636 2:-66324967 None:intergenic
GCCAAATTTGGAGCAACAAG+AGG 0.452022 2:+66324037 MsG0280010218.01.T01:CDS
ACCTTCCCTTTGTATGCTGT+CGG 0.452141 2:+66325143 MsG0280010218.01.T01:CDS
AGAGGAACCTTGTGTATGAT+TGG 0.454533 2:+66324519 MsG0280010218.01.T01:CDS
ACGAAAGCAAGAACAATCTC+TGG 0.455404 2:+66325093 MsG0280010218.01.T01:CDS
AACAACTTTGTGTCGTGGAC+AGG 0.455643 2:-66324175 None:intergenic
AGAGAGTTTGAGTTCTGAAA+AGG 0.456424 2:+66323430 MsG0280010218.01.T01:CDS
GTTAAAGAAGGATAGAGATT+CGG 0.464514 2:+66325190 MsG0280010218.01.T01:CDS
TGGACGACCAAGTTAGATAC+AGG 0.464742 2:-66325061 None:intergenic
TCAAGAAGTGAATTTGATGC+TGG 0.466884 2:+66324348 MsG0280010218.01.T01:CDS
CATATGTGATGGTAATTGAT+TGG 0.467774 2:-66323714 None:intergenic
ATCAAATTCACTTCTTGAGA+AGG 0.474088 2:-66324343 None:intergenic
TTTGATCCTTTGAATCTATG+AGG 0.475611 2:-66325578 None:intergenic
GGAATCGTGAACAATGATAA+TGG 0.480001 2:+66324369 MsG0280010218.01.T01:CDS
TCTAAACGTTTGAAGTAAAG+AGG 0.482986 2:-66324690 None:intergenic
ATTGCTGTTGAAGTTAAAGA+AGG 0.484181 2:+66325178 MsG0280010218.01.T01:CDS
AGTAAAGAGGATCGGATTCT+GGG 0.485124 2:-66324677 None:intergenic
ACTTCAAAACGATAACAGTT+AGG 0.487221 2:+66324076 MsG0280010218.01.T01:CDS
TTGGATCGGCATAGAAGTAT+AGG 0.492421 2:-66325448 None:intergenic
CCATCACATATGTCTTTGAA+AGG 0.493040 2:+66323725 MsG0280010218.01.T01:CDS
AGAAGTATAGGTCATTATCT+TGG 0.499463 2:-66325436 None:intergenic
TTCACTTCTTGAGAAGGAAA+CGG 0.499973 2:-66324337 None:intergenic
CATCACATATGTCTTTGAAA+GGG 0.501788 2:+66323726 MsG0280010218.01.T01:CDS
CCCATGACGGATCATGTTGT+CGG 0.501844 2:-66325235 None:intergenic
AGGGCTTGTTTACATGTTGG+TGG 0.508291 2:-66325642 None:intergenic
CATAGATTCAAAGGATCAAA+GGG 0.508834 2:+66325581 MsG0280010218.01.T01:CDS
AGGAAACGGAAGATGTAGAT+TGG 0.511543 2:-66324323 None:intergenic
TATGGTGGTTTGGTATTGGA+TGG 0.513398 2:+66325272 MsG0280010218.01.T01:CDS
AATGGACATGGAAGTTATCA+TGG 0.516707 2:+66324764 MsG0280010218.01.T01:CDS
TGACATGAAATTTGATATGT+CGG 0.517094 2:+66324236 MsG0280010218.01.T01:CDS
TTGTTGAGAACCAAGGGAAA+AGG 0.520792 2:+66324193 MsG0280010218.01.T01:CDS
CATTAGGGTTTCTCTTCACA+TGG 0.524442 2:-66324836 None:intergenic
TCATAGATTCAAAGGATCAA+AGG 0.525867 2:+66325580 MsG0280010218.01.T01:CDS
TACTCATCATCAACAAATGA+AGG 0.529729 2:-66324300 None:intergenic
AAAAGGGCTTGTTTACATGT+TGG 0.530079 2:-66325645 None:intergenic
ATTGATTAACTCTACCAAGT+TGG 0.530709 2:-66324152 None:intergenic
GATCATGTTGTCGGTAGACT+TGG 0.531155 2:-66325226 None:intergenic
CCGTGACCTTGTGTCAAACA+AGG 0.541599 2:+66324545 MsG0280010218.01.T01:CDS
GTGTTCCCTTGTTTGACACA+AGG 0.543475 2:-66324551 None:intergenic
TTCTCAACAACTTTGTGTCG+TGG 0.548134 2:-66324180 None:intergenic
ATAATCCGACAGCATACAAA+GGG 0.550306 2:-66325148 None:intergenic
TCCGTCTCCTGTATCTAACT+TGG 0.554874 2:+66325054 MsG0280010218.01.T01:CDS
AGGTATGAATATAGCCAACT+TGG 0.561427 2:+66324138 MsG0280010218.01.T01:CDS
CCTTGTTTGACACAAGGTCA+CGG 0.563302 2:-66324545 None:intergenic
TCGGTGAAGATTTCGGCAGA+AGG 0.566368 2:+66324483 MsG0280010218.01.T01:CDS
GTCAAAAGGCGAAGAAGACA+TGG 0.571754 2:-66324430 None:intergenic
CCAAATTTGGAGCAACAAGA+GGG 0.573396 2:+66324038 MsG0280010218.01.T01:CDS
GTTCTGCCTCATAGATTCAA+AGG 0.574597 2:+66325572 MsG0280010218.01.T01:CDS
GCTTCGAAATTGAGAACAAG+TGG 0.574778 2:-66324915 None:intergenic
ACTCATGAGTGTACTCTAGT+TGG 0.574884 2:-66323649 None:intergenic
GATGATGTGAATTATGGTGT+GGG 0.575675 2:+66324102 MsG0280010218.01.T01:CDS
ACCGACAACATGATCCGTCA+TGG 0.584585 2:+66325234 MsG0280010218.01.T01:CDS
ACGTTTGAAGTAAAGAGGAT+CGG 0.584769 2:-66324685 None:intergenic
TAGTGATGATGATGAGGATG+AGG 0.585316 2:+66323559 MsG0280010218.01.T01:CDS
GAAGGAGTTTATGATTCAAG+AGG 0.591580 2:+66324501 MsG0280010218.01.T01:CDS
AAATGTTTGGAGAATGGACA+TGG 0.592263 2:+66324752 MsG0280010218.01.T01:CDS
ATCATCAACAAATGAAGGAG+AGG 0.594337 2:-66324295 None:intergenic
ATCATGAAAATTGAGATGAA+GGG 0.595539 2:-66324864 None:intergenic
AATAATCCGACAGCATACAA+AGG 0.597844 2:-66325149 None:intergenic
CACAAAGTTGTTGAGAACCA+AGG 0.597900 2:+66324186 MsG0280010218.01.T01:CDS
CAGTCCAAAGAATTTGCTGT+TGG 0.600317 2:-66324573 None:intergenic
TGATGTGAATTATGGTGTGG+GGG 0.606449 2:+66324104 MsG0280010218.01.T01:CDS
GCAAAGGTGCCTGAATCAGA+AGG 0.607537 2:+66325608 MsG0280010218.01.T01:CDS
AACAAGTTCAGAATTACTTG+TGG 0.608075 2:-66325305 None:intergenic
ACATGCTTACGATCTTTACA+GGG 0.611348 2:+66325397 MsG0280010218.01.T01:CDS
CCTTTCAAAGACATATGTGA+TGG 0.614605 2:-66323725 None:intergenic
CCGACAACATGATCCGTCAT+GGG 0.615320 2:+66325235 MsG0280010218.01.T01:CDS
ACAAAGTTGTTGAGAACCAA+GGG 0.619367 2:+66324187 MsG0280010218.01.T01:CDS
TCGGTAGACTTGGTACATTG+AGG 0.622791 2:-66325216 None:intergenic
AGGATCAAAGGGATATGCAA+AGG 0.628643 2:+66325592 MsG0280010218.01.T01:CDS
ATGATGTGAATTATGGTGTG+GGG 0.631846 2:+66324103 MsG0280010218.01.T01:CDS
TCCGACAGCATACAAAGGGA+AGG 0.634652 2:-66325144 None:intergenic
AAGTGTTAGGCTAATCAGCA+TGG 0.635759 2:+66325013 MsG0280010218.01.T01:CDS
TGATGATGTGAATTATGGTG+TGG 0.636630 2:+66324101 MsG0280010218.01.T01:CDS
CTTCTAATAGCACTTGAGAG+AGG 0.638727 2:-66323623 None:intergenic
GATGTGAATTATGGTGTGGG+GGG 0.639439 2:+66324105 MsG0280010218.01.T01:CDS
GAGAACATAAAATCTTATGG+TGG 0.645989 2:+66325257 MsG0280010218.01.T01:CDS
GCAAGTACAATACCTACCAA+AGG 0.646564 2:-66325509 None:intergenic
AGTGATGATGATGAGGATGA+GGG 0.648880 2:+66323560 MsG0280010218.01.T01:CDS
CTGTAAAGATCGTAAGCATG+TGG 0.656477 2:-66325395 None:intergenic
CGTGACCTTGTGTCAAACAA+GGG 0.663371 2:+66324546 MsG0280010218.01.T01:CDS
ACCAAGTTAGATACAGGAGA+CGG 0.666053 2:-66325055 None:intergenic
ATAATAAGACATGTTAAACA+AGG 0.673949 2:-66324460 None:intergenic
GCGAAGAAGACATGGCTGAG+AGG 0.686145 2:-66324422 None:intergenic
CAAACGTTTAGAGGTATCTG+CGG 0.688357 2:+66324701 MsG0280010218.01.T01:CDS
ACGGCAACCAATCATACACA+AGG 0.708091 2:-66324526 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAGAAGAATAGGATTA+GGG + Chr2:66323763-66323782 MsG0280010218.01.T01:CDS 20.0%
!! AAGTTAATGAAATAAGTGTT+AGG + Chr2:66325000-66325019 MsG0280010218.01.T01:CDS 20.0%
!! AGCAAATTATAGTTATAGTT+TGG + Chr2:66323502-66323521 MsG0280010218.01.T01:CDS 20.0%
!! ATAATAAGACATGTTAAACA+AGG - Chr2:66324463-66324482 None:intergenic 20.0%
!! TTCAAGTAATATAACTAGTT+TGG + Chr2:66323394-66323413 MsG0280010218.01.T01:CDS 20.0%
!!! AAGATTTTTACTCAACTTAT+TGG + Chr2:66325474-66325493 MsG0280010218.01.T01:CDS 20.0%
!!! AATAAGTTGAGTAAAAATCT+TGG - Chr2:66325475-66325494 None:intergenic 20.0%
!!! AGATTTTTACTCAACTTATT+GGG + Chr2:66325475-66325494 MsG0280010218.01.T01:CDS 20.0%
!!! CTATAACTATAATTTGCTTT+TGG - Chr2:66323500-66323519 None:intergenic 20.0%
!!! GTTTAACATGTCTTATTATT+CGG + Chr2:66324464-66324483 MsG0280010218.01.T01:CDS 20.0%
!!! TATCTTTTGTATCTAAAGAA+GGG - Chr2:66324623-66324642 None:intergenic 20.0%
!!! TTTTAGGAAATGTTGAAAAA+TGG + Chr2:66324973-66324992 MsG0280010218.01.T01:CDS 20.0%
! AAAAATAGTAGTAGCATAGT+TGG + Chr2:66324015-66324034 MsG0280010218.01.T01:CDS 25.0%
! AAAAGATAGGAGCTATATTA+AGG + Chr2:66324635-66324654 MsG0280010218.01.T01:CDS 25.0%
! AAAGATAGGAGCTATATTAA+GGG + Chr2:66324636-66324655 MsG0280010218.01.T01:CDS 25.0%
! AATACCAAAACTATGTTGAT+AGG + Chr2:66323848-66323867 MsG0280010218.01.T01:intron 25.0%
! ATACCAAAACTATGTTGATA+GGG + Chr2:66323849-66323868 MsG0280010218.01.T01:intron 25.0%
! ATCATGAAAATTGAGATGAA+GGG - Chr2:66324867-66324886 None:intergenic 25.0%
! CAAAAAAGAAGAATAGGATT+AGG + Chr2:66323762-66323781 MsG0280010218.01.T01:CDS 25.0%
! CTGAATGAATCAAAGTTTAA+TGG - Chr2:66323590-66323609 None:intergenic 25.0%
! CTTCTTTAGATACAAAAGAT+AGG + Chr2:66324622-66324641 MsG0280010218.01.T01:CDS 25.0%
! GGAAACATCATTAATGAAAT+TGG - Chr2:66323479-66323498 None:intergenic 25.0%
! GTTCTTCAAAAAAGAAGAAT+AGG + Chr2:66323756-66323775 MsG0280010218.01.T01:CDS 25.0%
! TGACATGAAATTTGATATGT+CGG + Chr2:66324236-66324255 MsG0280010218.01.T01:CDS 25.0%
! TTCAACATTTCCTAAAACAT+AGG - Chr2:66324970-66324989 None:intergenic 25.0%
!! AATATAACTAGTTTGGTTAG+TGG + Chr2:66323401-66323420 MsG0280010218.01.T01:CDS 25.0%
!! CTATCTTTTGTATCTAAAGA+AGG - Chr2:66324624-66324643 None:intergenic 25.0%
!! TTATTATTCGGTGAAGATTT+CGG + Chr2:66324476-66324495 MsG0280010218.01.T01:CDS 25.0%
!!! CGAATAGTTTTGATTACTAT+TGG + Chr2:66323801-66323820 MsG0280010218.01.T01:intron 25.0%
!!! CTTTCATGCTCATTTTATTT+TGG + Chr2:66325350-66325369 MsG0280010218.01.T01:CDS 25.0%
!!! GTTTTGATTACTATTGGAAT+AGG + Chr2:66323807-66323826 MsG0280010218.01.T01:intron 25.0%
AACAAGGAATTCTTGTCAAA+AGG - Chr2:66324447-66324466 None:intergenic 30.0%
AACAAGTTCAGAATTACTTG+TGG - Chr2:66325308-66325327 None:intergenic 30.0%
AAGAAGAGAGAAGAAATGTT+TGG + Chr2:66324739-66324758 MsG0280010218.01.T01:CDS 30.0%
AATTGCACTCCTATAATTAG+TGG + Chr2:66323686-66323705 MsG0280010218.01.T01:CDS 30.0%
ACTTCAAAACGATAACAGTT+AGG + Chr2:66324076-66324095 MsG0280010218.01.T01:CDS 30.0%
ATCAAATTCACTTCTTGAGA+AGG - Chr2:66324346-66324365 None:intergenic 30.0%
ATGTCATATGAATATGCATC+TGG - Chr2:66324222-66324241 None:intergenic 30.0%
ATTGATTAACTCTACCAAGT+TGG - Chr2:66324155-66324174 None:intergenic 30.0%
CATAAAATCTTATGGTGGTT+TGG + Chr2:66325262-66325281 MsG0280010218.01.T01:CDS 30.0%
CATAGATTCAAAGGATCAAA+GGG + Chr2:66325581-66325600 MsG0280010218.01.T01:CDS 30.0%
CATATGTGATGGTAATTGAT+TGG - Chr2:66323717-66323736 None:intergenic 30.0%
CATCATGAAAATTGAGATGA+AGG - Chr2:66324868-66324887 None:intergenic 30.0%
GAGAACATAAAATCTTATGG+TGG + Chr2:66325257-66325276 MsG0280010218.01.T01:CDS 30.0%
GTTAAAGAAGGATAGAGATT+CGG + Chr2:66325190-66325209 MsG0280010218.01.T01:CDS 30.0%
TACTCATCATCAACAAATGA+AGG - Chr2:66324303-66324322 None:intergenic 30.0%
TCATAGATTCAAAGGATCAA+AGG + Chr2:66325580-66325599 MsG0280010218.01.T01:CDS 30.0%
TCTTTACTTCAAACGTTTAG+AGG + Chr2:66324692-66324711 MsG0280010218.01.T01:CDS 30.0%
TGGGAGAACATAAAATCTTA+TGG + Chr2:66325254-66325273 MsG0280010218.01.T01:CDS 30.0%
TTGGAACTTCCACTAATTAT+AGG - Chr2:66323698-66323717 None:intergenic 30.0%
TTGGCTATATTCATACCTTA+AGG - Chr2:66324136-66324155 None:intergenic 30.0%
TTTGATCCTTTGAATCTATG+AGG - Chr2:66325581-66325600 None:intergenic 30.0%
! AAAACATAGGTTGTGTTGTT+AGG - Chr2:66324957-66324976 None:intergenic 30.0%
! AACAACACAACCTATGTTTT+AGG + Chr2:66324957-66324976 MsG0280010218.01.T01:CDS 30.0%
! AAGAATAGGATTAGGGTTTT+GGG + Chr2:66323770-66323789 MsG0280010218.01.T01:intron 30.0%
! AGAAGTATAGGTCATTATCT+TGG - Chr2:66325439-66325458 None:intergenic 30.0%
! AGGAATGATGATGTGAATTA+TGG + Chr2:66324096-66324115 MsG0280010218.01.T01:CDS 30.0%
! ATTGCTGTTGAAGTTAAAGA+AGG + Chr2:66325178-66325197 MsG0280010218.01.T01:CDS 30.0%
! ATTTTTGATCGACCAAATTG+AGG - Chr2:66324001-66324020 None:intergenic 30.0%
! CATCACATATGTCTTTGAAA+GGG + Chr2:66323726-66323745 MsG0280010218.01.T01:CDS 30.0%
! GATATTTTCATTCCTTTGGT+AGG + Chr2:66325497-66325516 MsG0280010218.01.T01:CDS 30.0%
! TACCAAAACTATGTTGATAG+GGG + Chr2:66323850-66323869 MsG0280010218.01.T01:intron 30.0%
! TCTAAACGTTTGAAGTAAAG+AGG - Chr2:66324693-66324712 None:intergenic 30.0%
! TTGGGATATTTTCATTCCTT+TGG + Chr2:66325493-66325512 MsG0280010218.01.T01:CDS 30.0%
! TTTTTGATCGACCAAATTGA+GGG - Chr2:66324000-66324019 None:intergenic 30.0%
!! TAGATTTGCTACCATTGAAA+TGG - Chr2:66323922-66323941 None:intergenic 30.0%
!!! ATCTACTTGCAGCAATTTTT+TGG + Chr2:66325539-66325558 MsG0280010218.01.T01:CDS 30.0%
AAATGTTTGGAGAATGGACA+TGG + Chr2:66324752-66324771 MsG0280010218.01.T01:CDS 35.0%
AACACCAACAGCAAATTCTT+TGG + Chr2:66324569-66324588 MsG0280010218.01.T01:CDS 35.0%
AATAATCCGACAGCATACAA+AGG - Chr2:66325152-66325171 None:intergenic 35.0%
AATGGACATGGAAGTTATCA+TGG + Chr2:66324764-66324783 MsG0280010218.01.T01:CDS 35.0%
ACAAAGTTGTTGAGAACCAA+GGG + Chr2:66324187-66324206 MsG0280010218.01.T01:CDS 35.0%
ACATGCTTACGATCTTTACA+GGG + Chr2:66325397-66325416 MsG0280010218.01.T01:CDS 35.0%
AGATTGAGATTCCCTCAATT+TGG + Chr2:66323986-66324005 MsG0280010218.01.T01:CDS 35.0%
AGGTATGAATATAGCCAACT+TGG + Chr2:66324138-66324157 MsG0280010218.01.T01:CDS 35.0%
AGTCCAAAAAAAGAGTAGCT+TGG + Chr2:66324265-66324284 MsG0280010218.01.T01:CDS 35.0%
ATAATCCGACAGCATACAAA+GGG - Chr2:66325151-66325170 None:intergenic 35.0%
ATCATCAACAAATGAAGGAG+AGG - Chr2:66324298-66324317 None:intergenic 35.0%
ATGATGTGAATTATGGTGTG+GGG + Chr2:66324103-66324122 MsG0280010218.01.T01:CDS 35.0%
CCATCACATATGTCTTTGAA+AGG + Chr2:66323725-66323744 MsG0280010218.01.T01:CDS 35.0%
CCTTTCAAAGACATATGTGA+TGG - Chr2:66323728-66323747 None:intergenic 35.0%
GAAGGAGTTTATGATTCAAG+AGG + Chr2:66324501-66324520 MsG0280010218.01.T01:CDS 35.0%
GAGAAGAAATGTTTGGAGAA+TGG + Chr2:66324746-66324765 MsG0280010218.01.T01:CDS 35.0%
GATGATGTGAATTATGGTGT+GGG + Chr2:66324102-66324121 MsG0280010218.01.T01:CDS 35.0%
GGAATCGTGAACAATGATAA+TGG + Chr2:66324369-66324388 MsG0280010218.01.T01:CDS 35.0%
GTCCAAAAAAAGAGTAGCTT+GGG + Chr2:66324266-66324285 MsG0280010218.01.T01:CDS 35.0%
GTTGAGTAAAAATCTTGGCT+TGG - Chr2:66325470-66325489 None:intergenic 35.0%
TCAAGAAGTGAATTTGATGC+TGG + Chr2:66324348-66324367 MsG0280010218.01.T01:CDS 35.0%
TCCAAAAAAAGAGTAGCTTG+GGG + Chr2:66324267-66324286 MsG0280010218.01.T01:CDS 35.0%
TGGAATAGGAATAATAGCCA+AGG + Chr2:66323821-66323840 MsG0280010218.01.T01:intron 35.0%
TTCACTTCTTGAGAAGGAAA+CGG - Chr2:66324340-66324359 None:intergenic 35.0%
TTGTATCTAAAGAAGGGAAC+TGG - Chr2:66324617-66324636 None:intergenic 35.0%
! AAAACTATGTTGATAGGGGA+AGG + Chr2:66323854-66323873 MsG0280010218.01.T01:intron 35.0%
! ACGTTTGAAGTAAAGAGGAT+CGG - Chr2:66324688-66324707 None:intergenic 35.0%
! AGAATAGGATTAGGGTTTTG+GGG + Chr2:66323771-66323790 MsG0280010218.01.T01:intron 35.0%
! CACATATGAAGCTTTTCTCT+CGG - Chr2:66325123-66325142 None:intergenic 35.0%
! GAAGAATAGGATTAGGGTTT+TGG + Chr2:66323769-66323788 MsG0280010218.01.T01:intron 35.0%
! GATTTTATGTTCTCCCATGA+CGG - Chr2:66325251-66325270 None:intergenic 35.0%
! GTAGTTCTTTGCCATTTCAA+TGG + Chr2:66323908-66323927 MsG0280010218.01.T01:intron 35.0%
! GTTGAAAACGAGTTCTCTTT+TGG + Chr2:66323536-66323555 MsG0280010218.01.T01:CDS 35.0%
! TGATGATGTGAATTATGGTG+TGG + Chr2:66324101-66324120 MsG0280010218.01.T01:CDS 35.0%
! TTCCCCTATCAACATAGTTT+TGG - Chr2:66323855-66323874 None:intergenic 35.0%
!! AGAGAGTTTGAGTTCTGAAA+AGG + Chr2:66323430-66323449 MsG0280010218.01.T01:CDS 35.0%
!! AGCAAAGGTGTTTCGAAAAA+AGG + Chr2:66323323-66323342 MsG0280010218.01.T01:CDS 35.0%
!! ATCTTATGGTGGTTTGGTAT+TGG + Chr2:66325268-66325287 MsG0280010218.01.T01:CDS 35.0%
!! GTAAAAATCTTGGCTTGGAT+CGG - Chr2:66325465-66325484 None:intergenic 35.0%
!!! TAGTTTTGGTATTGAAGCCT+TGG - Chr2:66323841-66323860 None:intergenic 35.0%
!!! TTTTGGTAGTGATGATGATG+AGG + Chr2:66323553-66323572 MsG0280010218.01.T01:CDS 35.0%
AAGTAAAGAGGATCGGATTC+TGG - Chr2:66324681-66324700 None:intergenic 40.0%
AAGTGTTAGGCTAATCAGCA+TGG + Chr2:66325013-66325032 MsG0280010218.01.T01:CDS 40.0%
ACCAAGTTAGATACAGGAGA+CGG - Chr2:66325058-66325077 None:intergenic 40.0%
ACGAAAGCAAGAACAATCTC+TGG + Chr2:66325093-66325112 MsG0280010218.01.T01:CDS 40.0%
ACTCATGAGTGTACTCTAGT+TGG - Chr2:66323652-66323671 None:intergenic 40.0%
AGAGGAACCTTGTGTATGAT+TGG + Chr2:66324519-66324538 MsG0280010218.01.T01:CDS 40.0%
AGCAAATCTACATCCATGGA+TGG + Chr2:66323932-66323951 MsG0280010218.01.T01:intron 40.0%
AGGAAACGGAAGATGTAGAT+TGG - Chr2:66324326-66324345 None:intergenic 40.0%
AGGATCAAAGGGATATGCAA+AGG + Chr2:66325592-66325611 MsG0280010218.01.T01:CDS 40.0%
AGTAAAGAGGATCGGATTCT+GGG - Chr2:66324680-66324699 None:intergenic 40.0%
CACAAAGTTGTTGAGAACCA+AGG + Chr2:66324186-66324205 MsG0280010218.01.T01:CDS 40.0%
CACATGCTTACGATCTTTAC+AGG + Chr2:66325396-66325415 MsG0280010218.01.T01:CDS 40.0%
CATTAGGGTTTCTCTTCACA+TGG - Chr2:66324839-66324858 None:intergenic 40.0%
CTGTAAAGATCGTAAGCATG+TGG - Chr2:66325398-66325417 None:intergenic 40.0%
CTTCTAATAGCACTTGAGAG+AGG - Chr2:66323626-66323645 None:intergenic 40.0%
GCAAATCTACATCCATGGAT+GGG + Chr2:66323933-66323952 MsG0280010218.01.T01:intron 40.0%
GCAAGTACAATACCTACCAA+AGG - Chr2:66325512-66325531 None:intergenic 40.0%
GCTTCGAAATTGAGAACAAG+TGG - Chr2:66324918-66324937 None:intergenic 40.0%
GTAGCATAGTTGGCCAAATT+TGG + Chr2:66324025-66324044 MsG0280010218.01.T01:CDS 40.0%
GTTCTGCCTCATAGATTCAA+AGG + Chr2:66325572-66325591 MsG0280010218.01.T01:CDS 40.0%
TCTACTTCTCCTTCTGATTC+AGG - Chr2:66325620-66325639 None:intergenic 40.0%
TGATGTGAATTATGGTGTGG+GGG + Chr2:66324104-66324123 MsG0280010218.01.T01:CDS 40.0%
TGGTAGCAAATCTACATCCA+TGG + Chr2:66323928-66323947 MsG0280010218.01.T01:intron 40.0%
TTCTCAACAACTTTGTGTCG+TGG - Chr2:66324183-66324202 None:intergenic 40.0%
TTGTTGAGAACCAAGGGAAA+AGG + Chr2:66324193-66324212 MsG0280010218.01.T01:CDS 40.0%
! ACTCTAGCATGTGTTTTCGT+CGG + Chr2:66324801-66324820 MsG0280010218.01.T01:CDS 40.0%
! AGATGAAGGGAAGCACATTA+GGG - Chr2:66324854-66324873 None:intergenic 40.0%
! AGTGATGATGATGAGGATGA+GGG + Chr2:66323560-66323579 MsG0280010218.01.T01:CDS 40.0%
! CCAAATTTGGAGCAACAAGA+GGG + Chr2:66324038-66324057 MsG0280010218.01.T01:CDS 40.0%
! CTTGCTTTCGTCAGTTTTTC+TGG - Chr2:66325084-66325103 None:intergenic 40.0%
! TAGTGATGATGATGAGGATG+AGG + Chr2:66323559-66323578 MsG0280010218.01.T01:CDS 40.0%
! TTGGATCGGCATAGAAGTAT+AGG - Chr2:66325451-66325470 None:intergenic 40.0%
!! ATGATGTCGATTCTCACGTT+TGG + Chr2:66324882-66324901 MsG0280010218.01.T01:CDS 40.0%
!! CAAACGTTTAGAGGTATCTG+CGG + Chr2:66324701-66324720 MsG0280010218.01.T01:CDS 40.0%
!! CAGTCCAAAGAATTTGCTGT+TGG - Chr2:66324576-66324595 None:intergenic 40.0%
!! GAATAGGATTAGGGTTTTGG+GGG + Chr2:66323772-66323791 MsG0280010218.01.T01:intron 40.0%
!! TATGGTGGTTTGGTATTGGA+TGG + Chr2:66325272-66325291 MsG0280010218.01.T01:CDS 40.0%
!!! ACCCCAAGCTACTCTTTTTT+TGG - Chr2:66324271-66324290 None:intergenic 40.0%
AACAACTTTGTGTCGTGGAC+AGG - Chr2:66324178-66324197 None:intergenic 45.0%
ACCTTCCCTTTGTATGCTGT+CGG + Chr2:66325143-66325162 MsG0280010218.01.T01:CDS 45.0%
ACGGCAACCAATCATACACA+AGG - Chr2:66324529-66324548 None:intergenic 45.0%
CCCTCTTGTTGCTCCAAATT+TGG - Chr2:66324041-66324060 None:intergenic 45.0%
CGTGACCTTGTGTCAAACAA+GGG + Chr2:66324546-66324565 MsG0280010218.01.T01:CDS 45.0%
GATCATGTTGTCGGTAGACT+TGG - Chr2:66325229-66325248 None:intergenic 45.0%
GATGTGAATTATGGTGTGGG+GGG + Chr2:66324105-66324124 MsG0280010218.01.T01:CDS 45.0%
GTCAAAAGGCGAAGAAGACA+TGG - Chr2:66324433-66324452 None:intergenic 45.0%
TCCGTCTCCTGTATCTAACT+TGG + Chr2:66325054-66325073 MsG0280010218.01.T01:CDS 45.0%
TGGACGACCAAGTTAGATAC+AGG - Chr2:66325064-66325083 None:intergenic 45.0%
! CATCTGGATACCTTTTCCCT+TGG - Chr2:66324206-66324225 None:intergenic 45.0%
! CCTTGTTTGACACAAGGTCA+CGG - Chr2:66324548-66324567 None:intergenic 45.0%
! CTATGTTGATAGGGGAAGGA+TGG + Chr2:66323858-66323877 MsG0280010218.01.T01:intron 45.0%
! GAGATGAAGGGAAGCACATT+AGG - Chr2:66324855-66324874 None:intergenic 45.0%
! GCCAAATTTGGAGCAACAAG+AGG + Chr2:66324037-66324056 MsG0280010218.01.T01:CDS 45.0%
! GTGTTCCCTTGTTTGACACA+AGG - Chr2:66324554-66324573 None:intergenic 45.0%
! TCGGTAGACTTGGTACATTG+AGG - Chr2:66325219-66325238 None:intergenic 45.0%
ACCAACATGAGTCCCATCCA+TGG - Chr2:66323948-66323967 None:intergenic 50.0%
ACCGACAACATGATCCGTCA+TGG + Chr2:66325234-66325253 MsG0280010218.01.T01:CDS 50.0%
CCGACAACATGATCCGTCAT+GGG + Chr2:66325235-66325254 MsG0280010218.01.T01:CDS 50.0%
CCGTGACCTTGTGTCAAACA+AGG + Chr2:66324545-66324564 MsG0280010218.01.T01:CDS 50.0%
TCCATGGATGGGACTCATGT+TGG + Chr2:66323944-66323963 MsG0280010218.01.T01:intron 50.0%
TCCGACAGCATACAAAGGGA+AGG - Chr2:66325147-66325166 None:intergenic 50.0%
TCGGTGAAGATTTCGGCAGA+AGG + Chr2:66324483-66324502 MsG0280010218.01.T01:CDS 50.0%
! GTTGATAGGGGAAGGATGGT+GGG + Chr2:66323862-66323881 MsG0280010218.01.T01:intron 50.0%
! TGTTGATAGGGGAAGGATGG+TGG + Chr2:66323861-66323880 MsG0280010218.01.T01:intron 50.0%
!! CCCATGACGGATCATGTTGT+CGG - Chr2:66325238-66325257 None:intergenic 50.0%
!! GCAAAGGTGCCTGAATCAGA+AGG + Chr2:66325608-66325627 MsG0280010218.01.T01:CDS 50.0%
GCGAAGAAGACATGGCTGAG+AGG - Chr2:66324425-66324444 None:intergenic 55.0%
GTGTGGGGGGTAAAGCCTTA+AGG + Chr2:66324118-66324137 MsG0280010218.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr2 gene 66323311 66325655 66323311 ID=MsG0280010218.01;Name=MsG0280010218.01
Chr2 mRNA 66323311 66325655 66323311 ID=MsG0280010218.01.T01;Parent=MsG0280010218.01;Name=MsG0280010218.01.T01;_AED=0.33;_eAED=0.33;_QI=0|0|0|1|1|1|2|0|715
Chr2 exon 66323311 66323784 66323311 ID=MsG0280010218.01.T01:exon:5294;Parent=MsG0280010218.01.T01
Chr2 exon 66323982 66325655 66323982 ID=MsG0280010218.01.T01:exon:5295;Parent=MsG0280010218.01.T01
Chr2 CDS 66323311 66323784 66323311 ID=MsG0280010218.01.T01:cds;Parent=MsG0280010218.01.T01
Chr2 CDS 66323982 66325655 66323982 ID=MsG0280010218.01.T01:cds;Parent=MsG0280010218.01.T01
Gene Sequence

>MsG0280010218.01.T01

ATGGTTGGAAAAAGCAAAGGTGTTTCGAAAAAAGGTGATTCTCTCAATCTTGATGTTGTTTTTAAACTTAACAATGTCTTCGATTCAAGTAATATAACTAGTTTGGTTAGTGGAAGCTTAGAGAGTTTGAGTTCTGAAAAGGATCATGATGATCATTACTTTGAACCAATTTCATTAATGATGTTTCCAAAAGCAAATTATAGTTATAGTTTGGATTCTAAAGAAGTTGAAAACGAGTTCTCTTTTGGTAGTGATGATGATGAGGATGAGGGTTTACCATTAAACTTTGATTCATTCAGTTTTTGTAAATATCCTCTCTCAAGTGCTATTAGAAGACTCCAACTAGAGTACACTCATGAGTGCAATTCTTCAAAGAATTGCACTCCTATAATTAGTGGAAGTTCCAATCAATTACCATCACATATGTCTTTGAAAGGGATTGAATGTTCTTCAAAAAAGAAGAATAGGATTAGGATTAAGATTGAGATTCCCTCAATTTGGTCGATCAAAAATAGTAGTAGCATAGTTGGCCAAATTTGGAGCAACAAGAGGGATAATGATCAAAACTACTTCAAAACGATAACAGTTAGGAATGATGATGTGAATTATGGTGTGGGGGGTAAAGCCTTAAGGTATGAATATAGCCAACTTGGTAGAGTTAATCAATCCTGTCCACGACACAAAGTTGTTGAGAACCAAGGGAAAAGGTATCCAGATGCATATTCATATGACATGAAATTTGATATGTCGGTTAGAGAGTCCAAAAAAAGAGTAGCTTGGGGTTATTCCTCTCCTTCATTTGTTGATGATGAGTACCAATCTACATCTTCCGTTTCCTTCTCAAGAAGTGAATTTGATGCTGGAATCGTGAACAATGATAATGGTAGCTTGTTTAACATTAGCTACAAGATTAGCCTCTCAGCCATGTCTTCTTCGCCTTTTGACAAGAATTCCTTGTTTAACATGTCTTATTATTCGGTGAAGATTTCGGCAGAAGGAGTTTATGATTCAAGAGGAACCTTGTGTATGATTGGTTGCCGTGACCTTGTGTCAAACAAGGGAACACCAACAGCAAATTCTTTGGACTGTGAGATTCTGATGAAATTCCAGTTCCCTTCTTTAGATACAAAAGATAGGAGCTATATTAAGGGAAGCATTGAAAGCACGCGCCCAGAATCCGATCCTCTTTACTTCAAACGTTTAGAGGTATCTGCGGTAGCGTATTACATCAAAGAAGAGAGAAGAAATGTTTGGAGAATGGACATGGAAGTTATCATGGCTCTGATATCTACAACTCTAGCATGTGTTTTCGTCGGATTGCAACTCAACCATGTGAAGAGAAACCCTAATGTGCTTCCCTTCATCTCAATTTTCATGATGTCGATTCTCACGTTTGGTCACATGATACCACTTGTTCTCAATTTCGAAGCACTTCTTGCTAAAAATCCTAACAACACAACCTATGTTTTAGGAAATGTTGAAAAATGGCTTGAAGTTAATGAAATAAGTGTTAGGCTAATCAGCATGGTAGCTTTCTTATTGCAATTCCGTCTCCTGTATCTAACTTGGTCGTCCAGAAAAACTGACGAAAGCAAGAACAATCTCTGGATTGCCGAGAGAAAAGCTTCATATGTGACCTTCCCTTTGTATGCTGTCGGATTATTGATTGCATTGCTGTTGAAGTTAAAGAAGGATAGAGATTCGGTTACCTCAATGTACCAAGTCTACCGACAACATGATCCGTCATGGGAGAACATAAAATCTTATGGTGGTTTGGTATTGGATGGCTTTCTTGTGCCACAAGTAATTCTGAACTTGTTTTCAAATATGAATGAGAATGTTCTTTCATGCTCATTTTATTTTGGAACTACTTTTGTGAGACTCTTGCCACATGCTTACGATCTTTACAGGGCTCATAATTATGCTGACCAAGATAATGACCTATACTTCTATGCCGATCCAAGCCAAGATTTTTACTCAACTTATTGGGATATTTTCATTCCTTTGGTAGGTATTGTACTTGCTATCATTATCTACTTGCAGCAATTTTTTGGTGCTCAATGTGTTCTGCCTCATAGATTCAAAGGATCAAAGGGATATGCAAAGGTGCCTGAATCAGAAGGAGAAGTAGAGACCACCAACATGTAA
Protein sequence

>MsG0280010218.01.T01

MVGKSKGVSKKGDSLNLDVVFKLNNVFDSSNITSLVSGSLESLSSEKDHDDHYFEPISLMMFPKANYSYSLDSKEVENEFSFGSDDDEDEGLPLNFDSFSFCKYPLSSAIRRLQLEYTHECNSSKNCTPIISGSSNQLPSHMSLKGIECSSKKKNRIRIKIEIPSIWSIKNSSSIVGQIWSNKRDNDQNYFKTITVRNDDVNYGVGGKALRYEYSQLGRVNQSCPRHKVVENQGKRYPDAYSYDMKFDMSVRESKKRVAWGYSSPSFVDDEYQSTSSVSFSRSEFDAGIVNNDNGSLFNISYKISLSAMSSSPFDKNSLFNMSYYSVKISAEGVYDSRGTLCMIGCRDLVSNKGTPTANSLDCEILMKFQFPSLDTKDRSYIKGSIESTRPESDPLYFKRLEVSAVAYYIKEERRNVWRMDMEVIMALISTTLACVFVGLQLNHVKRNPNVLPFISIFMMSILTFGHMIPLVLNFEALLAKNPNNTTYVLGNVEKWLEVNEISVRLISMVAFLLQFRLLYLTWSSRKTDESKNNLWIAERKASYVTFPLYAVGLLIALLLKLKKDRDSVTSMYQVYRQHDPSWENIKSYGGLVLDGFLVPQVILNLFSNMNENVLSCSFYFGTTFVRLLPHAYDLYRAHNYADQDNDLYFYADPSQDFYSTYWDIFIPLVGIVLAIIIYLQQFFGAQCVLPHRFKGSKGYAKVPESEGEVETTNM*