AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010533.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010533.01.T01 MTR_2g086950 95.506 89 4 0 1 89 1 89 7.57e-52 168
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010533.01.T01 AT5G22320 65.169 89 31 0 1 89 1 89 5.06e-34 121
MsG0280010533.01.T01 AT5G22320 65.169 89 31 0 1 89 1 89 6.56e-34 120

Find 17 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTAGCCAGCTTCAATAAAT+TGG 0.169541 2:+71048458 MsG0280010533.01.T01:CDS
TTCCTCACTGCACTCATTCA+AGG 0.388703 2:-71048230 None:intergenic
GGAGAATAAATTAGAAAGCT+TGG 0.390492 2:+71048663 MsG0280010533.01.T01:CDS
GAAAGCTTGGAAGGAATTCA+AGG 0.449690 2:+71048676 MsG0280010533.01.T01:CDS
TTTGAAGTGGTTATCAGTTG+TGG 0.468829 2:+71048642 MsG0280010533.01.T01:CDS
TGAAGACTGGAAATTGAACT+TGG 0.471828 2:-71048307 None:intergenic
TATGGTGTAAAAGGGATTGA+GGG 0.493320 2:+71048609 MsG0280010533.01.T01:intron
ACTGGAAATTGAACTTGGAT+TGG 0.499943 2:-71048302 None:intergenic
GTATGGTGTAAAAGGGATTG+AGG 0.510413 2:+71048608 MsG0280010533.01.T01:intron
GGAAATTGAACTTGGATTGG+CGG 0.520167 2:-71048299 None:intergenic
AATAAATTAGAAAGCTTGGA+AGG 0.526330 2:+71048667 MsG0280010533.01.T01:CDS
GCTGTCGTTCAATACTTGCT+CGG 0.536067 2:-71048272 None:intergenic
CAATAATCTCACTTCACTTG+AGG 0.539721 2:+71048500 MsG0280010533.01.T01:CDS
CGGAAAGAGCTTTGTGAGTG+AGG 0.545351 2:-71048330 None:intergenic
TTTGTGAGTGAGGTGAAGAC+TGG 0.573523 2:-71048320 None:intergenic
GGGCATGTGTTAATTTGAAG+TGG 0.617171 2:+71048629 MsG0280010533.01.T01:CDS
TGCCTTGAATGAGTGCAGTG+AGG 0.768971 2:+71048228 MsG0280010533.01.T01:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAAAGTTAAGATTTTTT+AGG - Chr2:71048410-71048429 None:intergenic 10.0%
!!! AAAAAAGTTAAGATTTTTTA+GGG - Chr2:71048409-71048428 None:intergenic 10.0%
!! TGAAAGTTATGAAAAAAAAT+TGG + Chr2:71048552-71048571 MsG0280010533.01.T01:intron 15.0%
!!! TTGATTGTATTTTATATGTA+TGG + Chr2:71048591-71048610 MsG0280010533.01.T01:intron 15.0%
!! ATGAAAAAAAATTGGAACTT+TGG + Chr2:71048560-71048579 MsG0280010533.01.T01:intron 20.0%
!! TTTATATGTATGGTGTAAAA+GGG + Chr2:71048601-71048620 MsG0280010533.01.T01:intron 20.0%
!!! TTTTATATGTATGGTGTAAA+AGG + Chr2:71048600-71048619 MsG0280010533.01.T01:intron 20.0%
!!! TTTTTAGGGTTTTAACAAAA+GGG - Chr2:71048395-71048414 None:intergenic 20.0%
!!! TTTTTTAGGGTTTTAACAAA+AGG - Chr2:71048396-71048415 None:intergenic 20.0%
! AATAAATTAGAAAGCTTGGA+AGG + Chr2:71048667-71048686 MsG0280010533.01.T01:CDS 25.0%
!!! TTTTAGGGTTTTAACAAAAG+GGG - Chr2:71048394-71048413 None:intergenic 25.0%
GGAGAATAAATTAGAAAGCT+TGG + Chr2:71048663-71048682 MsG0280010533.01.T01:CDS 30.0%
TTAATCTTTGTTCATTTCCC+AGG + Chr2:71048428-71048447 MsG0280010533.01.T01:intron 30.0%
! CTTTTCCAATTTATTGAAGC+TGG - Chr2:71048466-71048485 None:intergenic 30.0%
ACTGGAAATTGAACTTGGAT+TGG - Chr2:71048305-71048324 None:intergenic 35.0%
AGGGGTTTAGAGAAAAGAAA+AGG - Chr2:71048376-71048395 None:intergenic 35.0%
CAATAATCTCACTTCACTTG+AGG + Chr2:71048500-71048519 MsG0280010533.01.T01:CDS 35.0%
CTTAGCCAGCTTCAATAAAT+TGG + Chr2:71048458-71048477 MsG0280010533.01.T01:CDS 35.0%
GGGGTTTAGAGAAAAGAAAA+GGG - Chr2:71048375-71048394 None:intergenic 35.0%
GGGTTTAGAGAAAAGAAAAG+GGG - Chr2:71048374-71048393 None:intergenic 35.0%
GGTTTAGAGAAAAGAAAAGG+GGG - Chr2:71048373-71048392 None:intergenic 35.0%
TATGGTGTAAAAGGGATTGA+GGG + Chr2:71048609-71048628 MsG0280010533.01.T01:intron 35.0%
TGAAGACTGGAAATTGAACT+TGG - Chr2:71048310-71048329 None:intergenic 35.0%
! TTTGAAGTGGTTATCAGTTG+TGG + Chr2:71048642-71048661 MsG0280010533.01.T01:CDS 35.0%
AGCTTACTGTAAGTACCTAG+AGG + Chr2:71048707-71048726 MsG0280010533.01.T01:CDS 40.0%
GAAAGCTTGGAAGGAATTCA+AGG + Chr2:71048676-71048695 MsG0280010533.01.T01:CDS 40.0%
GTATGGTGTAAAAGGGATTG+AGG + Chr2:71048608-71048627 MsG0280010533.01.T01:intron 40.0%
TTAGAGAAAAGAAAAGGGGG+TGG - Chr2:71048370-71048389 None:intergenic 40.0%
! GGAAATTGAACTTGGATTGG+CGG - Chr2:71048302-71048321 None:intergenic 40.0%
! GGGCATGTGTTAATTTGAAG+TGG + Chr2:71048629-71048648 MsG0280010533.01.T01:CDS 40.0%
AAGAAAAGGGGGTGGAAAGA+AGG - Chr2:71048362-71048381 None:intergenic 45.0%
GCTGTCGTTCAATACTTGCT+CGG - Chr2:71048275-71048294 None:intergenic 45.0%
GGGTGGAAAGAAGGTTACAT+CGG - Chr2:71048353-71048372 None:intergenic 45.0%
TTCCTCACTGCACTCATTCA+AGG - Chr2:71048233-71048252 None:intergenic 45.0%
TTTGTGAGTGAGGTGAAGAC+TGG - Chr2:71048323-71048342 None:intergenic 45.0%
AGCTGGCTAAGCATGATACC+TGG - Chr2:71048449-71048468 None:intergenic 50.0%
GCTGGCTAAGCATGATACCT+GGG - Chr2:71048448-71048467 None:intergenic 50.0%
TGCCTTGAATGAGTGCAGTG+AGG + Chr2:71048228-71048247 MsG0280010533.01.T01:five_prime_UTR 50.0%
! CGGAAAGAGCTTTGTGAGTG+AGG - Chr2:71048333-71048352 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr2 gene 71048200 71048726 71048200 ID=MsG0280010533.01;Name=MsG0280010533.01
Chr2 mRNA 71048200 71048726 71048200 ID=MsG0280010533.01.T01;Parent=MsG0280010533.01;Name=MsG0280010533.01.T01;_AED=0.49;_eAED=0.50;_QI=56|1|0.66|1|0.5|0.66|3|0|91
Chr2 exon 71048200 71048354 71048200 ID=MsG0280010533.01.T01:exon:10389;Parent=MsG0280010533.01.T01
Chr2 exon 71048450 71048521 71048450 ID=MsG0280010533.01.T01:exon:10390;Parent=MsG0280010533.01.T01
Chr2 exon 71048622 71048726 71048622 ID=MsG0280010533.01.T01:exon:10391;Parent=MsG0280010533.01.T01
Chr2 five_prime_UTR 71048200 71048255 71048200 ID=MsG0280010533.01.T01:five_prime_utr;Parent=MsG0280010533.01.T01
Chr2 CDS 71048256 71048354 71048256 ID=MsG0280010533.01.T01:cds;Parent=MsG0280010533.01.T01
Chr2 CDS 71048450 71048521 71048450 ID=MsG0280010533.01.T01:cds;Parent=MsG0280010533.01.T01
Chr2 CDS 71048622 71048726 71048622 ID=MsG0280010533.01.T01:cds;Parent=MsG0280010533.01.T01
Gene Sequence

>MsG0280010533.01.T01

ATGACTCGTTTGAGCTCCGAGCAAGTATTGAACGACAGCAATGCCGCCAATCCAAGTTCAATTTCCAGTCTTCACCTCACTCACAAAGCTCTTTCCGATGTATCATGCTTAGCCAGCTTCAATAAATTGGAAAAGCTTGATCTAAAATTCAATAATCTCACTTCACTTGAGGGATTGAGGGCATGTGTTAATTTGAAGTGGTTATCAGTTGTGGAGAATAAATTAGAAAGCTTGGAAGGAATTCAAGGACTTACTAAGCTTACTGTAAGTACCTAG

Protein sequence

>MsG0280010533.01.T01

MTRLSSEQVLNDSNAANPSSISSLHLTHKALSDVSCLASFNKLEKLDLKFNNLTSLEGLRACVNLKWLSVVENKLESLEGIQGLTKLTVST*