Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010667.01.T01 | XP_013465011.1 | 89.13 | 92 | 5 | 2 | 1 | 87 | 1 | 92 | 1.26E-44 | 149 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010667.01.T01 | A0A072VBQ8 | 89.130 | 92 | 5 | 2 | 1 | 87 | 1 | 92 | 6.03e-45 | 149 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003430.01 | MsG0280010667.01 | 0.807741 | 4.358826e-50 | 2.447060e-47 |
| MsG0280010663.01 | MsG0280010667.01 | 0.860317 | 2.246835e-63 | 6.115940e-60 |
| MsG0280010666.01 | MsG0280010667.01 | 0.841914 | 3.558075e-58 | 5.306348e-55 |
| MsG0280010667.01 | MsG0280010670.01 | 0.917845 | 3.229526e-86 | 9.533306e-82 |
| MsG0280010667.01 | MsG0280010671.01 | 0.809225 | 2.101628e-50 | 1.227001e-47 |
| MsG0280010667.01 | MsG0380017730.01 | 0.802694 | 4.968907e-49 | 2.449962e-46 |
| MsG0280010667.01 | MsG0480023360.01 | 0.803459 | 3.450506e-49 | 1.734928e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010667.01.T01 | MTR_2g089905 | 89.130 | 92 | 5 | 2 | 1 | 87 | 1 | 92 | 1.53e-48 | 149 |
| MsG0280010667.01.T01 | MTR_2g089940 | 84.615 | 91 | 10 | 1 | 1 | 87 | 1 | 91 | 7.08e-46 | 142 |
| MsG0280010667.01.T01 | MTR_2g089985 | 84.615 | 91 | 10 | 1 | 1 | 87 | 1 | 91 | 7.08e-46 | 142 |
| MsG0280010667.01.T01 | MTR_2g089960 | 84.615 | 91 | 10 | 1 | 1 | 87 | 1 | 91 | 7.08e-46 | 142 |
| MsG0280010667.01.T01 | MTR_2g089965 | 78.125 | 96 | 12 | 1 | 1 | 87 | 1 | 96 | 2.42e-42 | 133 |
| MsG0280010667.01.T01 | MTR_4g045990 | 74.157 | 89 | 21 | 2 | 1 | 87 | 1 | 89 | 2.72e-36 | 118 |
| MsG0280010667.01.T01 | MTR_2g089970 | 65.169 | 89 | 29 | 1 | 1 | 87 | 1 | 89 | 7.94e-36 | 117 |
| MsG0280010667.01.T01 | MTR_2g089990 | 65.169 | 89 | 29 | 1 | 1 | 87 | 1 | 89 | 1.37e-34 | 114 |
| MsG0280010667.01.T01 | MTR_2g089945 | 64.368 | 87 | 30 | 1 | 2 | 87 | 5 | 91 | 5.69e-34 | 112 |
| MsG0280010667.01.T01 | MTR_2g089930 | 60.920 | 87 | 33 | 1 | 2 | 87 | 5 | 91 | 4.23e-33 | 110 |
| MsG0280010667.01.T01 | MTR_2g089950 | 60.674 | 89 | 28 | 2 | 1 | 87 | 1 | 84 | 8.97e-30 | 101 |
| MsG0280010667.01.T01 | MTR_2g089925 | 58.427 | 89 | 30 | 2 | 1 | 87 | 1 | 84 | 2.45e-27 | 95.5 |
| MsG0280010667.01.T01 | MTR_2g481150 | 57.471 | 87 | 33 | 2 | 1 | 86 | 1 | 84 | 5.20e-27 | 94.7 |
| MsG0280010667.01.T01 | MTR_2g481160 | 60.227 | 88 | 29 | 3 | 1 | 86 | 1 | 84 | 7.98e-27 | 94.4 |
| MsG0280010667.01.T01 | MTR_2g089865 | 59.036 | 83 | 33 | 1 | 2 | 83 | 5 | 87 | 5.53e-24 | 92.0 |
| MsG0280010667.01.T01 | MTR_2g081155 | 51.685 | 89 | 33 | 5 | 1 | 86 | 1 | 82 | 2.76e-22 | 82.8 |
| MsG0280010667.01.T01 | MTR_2g089835 | 47.727 | 88 | 45 | 1 | 1 | 87 | 1 | 88 | 1.39e-21 | 80.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010667.01.T01 | AT4G10265 | 58.621 | 87 | 30 | 2 | 1 | 86 | 1 | 82 | 1.19e-30 | 103 |
| MsG0280010667.01.T01 | AT4G10270 | 61.111 | 90 | 32 | 2 | 1 | 87 | 1 | 90 | 1.64e-30 | 103 |
Find 17 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCAATCAGAGGAGTCTTTA+AGG | 0.273830 | 2:+73136740 | MsG0280010667.01.T01:CDS |
| AGTTATGTACTTGAGTTGTT+GGG | 0.380679 | 2:+73136765 | MsG0280010667.01.T01:CDS |
| TTCATCTTCTTCTTCTCCTA+TGG | 0.383759 | 2:+73136690 | MsG0280010667.01.T01:CDS |
| AAGTTATGTACTTGAGTTGT+TGG | 0.395020 | 2:+73136764 | MsG0280010667.01.T01:CDS |
| CAACCAAAGCTCCTCTTAGT+TGG | 0.412441 | 2:-73136791 | None:intergenic |
| TTCTAATTCTAGACAACAAA+AGG | 0.422761 | 2:+73136714 | MsG0280010667.01.T01:CDS |
| AACCAAAGCTCCTCTTAGTT+GGG | 0.489304 | 2:-73136790 | None:intergenic |
| AGCAGAGCATGGGTAGCTGC+AGG | 0.497606 | 2:+73136547 | MsG0280010667.01.T01:CDS |
| GTGGAAGCATTGAAAGATCA+AGG | 0.506795 | 2:+73136583 | MsG0280010667.01.T01:CDS |
| TTTGAACTGACTTTAGGGTA+TGG | 0.511934 | 2:-73136621 | None:intergenic |
| GTTATGTACTTGAGTTGTTG+GGG | 0.552188 | 2:+73136766 | MsG0280010667.01.T01:CDS |
| GAGTTCAACAAGCAGAGCAT+GGG | 0.554434 | 2:+73136537 | MsG0280010667.01.T01:CDS |
| TGCAGGCAGTGTTGCAGTTG+TGG | 0.558647 | 2:+73136564 | MsG0280010667.01.T01:CDS |
| TGAGTTCAACAAGCAGAGCA+TGG | 0.560896 | 2:+73136536 | MsG0280010667.01.T01:CDS |
| TGTCTAGAATTAGAAACCAT+AGG | 0.610973 | 2:-73136706 | None:intergenic |
| AAGATCAAGGAATATGCAGA+TGG | 0.613727 | 2:+73136596 | MsG0280010667.01.T01:CDS |
| ACAAAAGGCAAAGCAATCAG+AGG | 0.651878 | 2:+73136729 | MsG0280010667.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTCTAATTCTAGACAACAAA+AGG | + | Chr2:73136714-73136733 | MsG0280010667.01.T01:CDS | 25.0% |
| ! | AAGTTATGTACTTGAGTTGT+TGG | + | Chr2:73136764-73136783 | MsG0280010667.01.T01:CDS | 30.0% |
| ! | AGTTATGTACTTGAGTTGTT+GGG | + | Chr2:73136765-73136784 | MsG0280010667.01.T01:CDS | 30.0% |
| ! | TGTCTAGAATTAGAAACCAT+AGG | - | Chr2:73136709-73136728 | None:intergenic | 30.0% |
| !! | GTGATTTTGAACTGACTTTA+GGG | - | Chr2:73136629-73136648 | None:intergenic | 30.0% |
| AAGATCAAGGAATATGCAGA+TGG | + | Chr2:73136596-73136615 | MsG0280010667.01.T01:CDS | 35.0% | |
| TTCATCTTCTTCTTCTCCTA+TGG | + | Chr2:73136690-73136709 | MsG0280010667.01.T01:CDS | 35.0% | |
| ! | GTTATGTACTTGAGTTGTTG+GGG | + | Chr2:73136766-73136785 | MsG0280010667.01.T01:CDS | 35.0% |
| !! | CGTGATTTTGAACTGACTTT+AGG | - | Chr2:73136630-73136649 | None:intergenic | 35.0% |
| !! | TTTGAACTGACTTTAGGGTA+TGG | - | Chr2:73136624-73136643 | None:intergenic | 35.0% |
| ACAAAAGGCAAAGCAATCAG+AGG | + | Chr2:73136729-73136748 | MsG0280010667.01.T01:CDS | 40.0% | |
| AGCAATCAGAGGAGTCTTTA+AGG | + | Chr2:73136740-73136759 | MsG0280010667.01.T01:CDS | 40.0% | |
| !! | GTGGAAGCATTGAAAGATCA+AGG | + | Chr2:73136583-73136602 | MsG0280010667.01.T01:CDS | 40.0% |
| GAGTTCAACAAGCAGAGCAT+GGG | + | Chr2:73136537-73136556 | MsG0280010667.01.T01:CDS | 45.0% | |
| TGAGTTCAACAAGCAGAGCA+TGG | + | Chr2:73136536-73136555 | MsG0280010667.01.T01:CDS | 45.0% | |
| TGCAGGCAGTGTTGCAGTTG+TGG | + | Chr2:73136564-73136583 | MsG0280010667.01.T01:CDS | 55.0% | |
| AGCAGAGCATGGGTAGCTGC+AGG | + | Chr2:73136547-73136566 | MsG0280010667.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 73136535 | 73136798 | 73136535 | ID=MsG0280010667.01;Name=MsG0280010667.01 |
| Chr2 | mRNA | 73136535 | 73136798 | 73136535 | ID=MsG0280010667.01.T01;Parent=MsG0280010667.01;Name=MsG0280010667.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|87 |
| Chr2 | exon | 73136535 | 73136798 | 73136535 | ID=MsG0280010667.01.T01:exon:19620;Parent=MsG0280010667.01.T01 |
| Chr2 | CDS | 73136535 | 73136798 | 73136535 | ID=MsG0280010667.01.T01:cds;Parent=MsG0280010667.01.T01 |
| Gene Sequence |
| Protein sequence |
