AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380017730.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017730.01.T01 MTR_3g111290 61.728 162 7 3 1 107 176 337 2.10e-48 157
MsG0380017730.01.T01 MTR_5g030770 72.289 83 19 1 1 79 159 241 1.89e-29 107
MsG0380017730.01.T01 MTR_5g030770 46.584 161 32 4 1 107 159 319 3.89e-29 107
MsG0380017730.01.T01 MTR_7g083900 74.545 55 14 0 1 55 289 343 1.33e-22 90.9
MsG0380017730.01.T01 MTR_7g083900 74.545 55 14 0 1 55 289 343 1.37e-22 90.9
MsG0380017730.01.T01 MTR_3g498695 72.727 55 15 0 1 55 301 355 5.32e-22 89.4
MsG0380017730.01.T01 MTR_3g498695 72.727 55 15 0 1 55 301 355 5.33e-22 89.4
MsG0380017730.01.T01 MTR_3g030700 55.932 59 26 0 1 59 199 257 3.85e-17 75.5
MsG0380017730.01.T01 MTR_1g112680 55.172 58 26 0 1 58 320 377 6.01e-16 72.4
MsG0380017730.01.T01 MTR_2g081040 53.448 58 27 0 1 58 180 237 8.23e-16 71.6
MsG0380017730.01.T01 MTR_3g104100 51.724 58 28 0 1 58 198 255 3.63e-15 69.7
MsG0380017730.01.T01 MTR_1g072320 54.902 51 23 0 1 51 473 523 3.95e-14 67.0
MsG0380017730.01.T01 MTR_2g038040 47.887 71 33 2 1 70 55 122 1.37e-12 62.0
MsG0380017730.01.T01 MTR_8g027495 51.852 54 26 0 2 55 462 515 2.34e-12 62.0
MsG0380017730.01.T01 MTR_7g117670 49.180 61 31 0 2 62 406 466 3.84e-12 61.2
MsG0380017730.01.T01 MTR_8g024790 50.000 50 25 0 2 51 295 344 4.73e-12 61.2
MsG0380017730.01.T01 MTR_8g067280 50.000 50 25 0 2 51 466 515 6.32e-12 60.8
MsG0380017730.01.T01 MTR_8g024770 50.000 50 25 0 2 51 283 332 9.23e-12 60.5
MsG0380017730.01.T01 MTR_4g057270 46.296 54 29 0 1 54 152 205 9.82e-12 60.1
MsG0380017730.01.T01 MTR_7g096350 46.552 58 31 0 2 59 359 416 1.24e-11 60.1
MsG0380017730.01.T01 MTR_5g030430 54.348 46 21 0 2 47 497 542 1.45e-11 59.7
MsG0380017730.01.T01 MTR_4g092700 42.188 64 37 0 1 64 161 224 1.97e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017730.01.T01 AT5G65640 63.830 94 26 2 1 86 181 274 1.58e-24 94.4
MsG0380017730.01.T01 AT5G65640 67.816 87 20 2 1 79 181 267 5.89e-24 94.0
MsG0380017730.01.T01 AT1G12860 61.429 70 27 0 1 70 270 339 8.26e-23 91.3
MsG0380017730.01.T01 AT1G12860 61.429 70 27 0 1 70 270 339 8.36e-23 91.7
MsG0380017730.01.T01 AT3G26744 74.545 55 14 0 1 55 310 364 1.60e-22 90.9
MsG0380017730.01.T01 AT3G26744 74.545 55 14 0 1 55 310 364 1.60e-22 90.9
MsG0380017730.01.T01 AT3G26744 74.545 55 14 0 1 55 310 364 1.60e-22 90.9
MsG0380017730.01.T01 AT3G26744 74.545 55 14 0 1 55 310 364 1.86e-22 90.5
MsG0380017730.01.T01 AT5G10570 57.292 96 33 3 1 92 154 245 2.26e-21 86.3
MsG0380017730.01.T01 AT2G16910 60.345 58 23 0 1 58 317 374 1.10e-16 74.3
MsG0380017730.01.T01 AT4G29930 48.387 62 31 1 1 61 57 118 1.50e-13 63.5
MsG0380017730.01.T01 AT4G29930 48.387 62 31 1 1 61 57 118 2.10e-13 63.9
MsG0380017730.01.T01 AT4G29930 48.387 62 31 1 1 61 57 118 2.19e-13 63.9
MsG0380017730.01.T01 AT4G29930 48.387 62 31 1 1 61 57 118 2.75e-13 63.5
MsG0380017730.01.T01 AT4G29930 48.387 62 31 1 1 61 57 118 3.50e-13 63.5
MsG0380017730.01.T01 AT4G29930 48.387 62 31 1 1 61 57 118 3.64e-13 63.5
MsG0380017730.01.T01 AT4G29930 48.387 62 31 1 1 61 57 118 3.64e-13 63.5
MsG0380017730.01.T01 AT2G46510 53.846 52 24 0 2 53 399 450 8.40e-13 63.2
MsG0380017730.01.T01 AT4G00870 49.057 53 27 0 2 54 253 305 4.47e-12 61.2
MsG0380017730.01.T01 AT1G32640 54.348 46 21 0 2 47 456 501 1.18e-11 60.1
MsG0380017730.01.T01 AT1G01260 48.148 54 28 0 2 55 437 490 2.13e-11 59.3
MsG0380017730.01.T01 AT1G01260 48.148 54 28 0 2 55 437 490 2.13e-11 59.3
MsG0380017730.01.T01 AT1G01260 48.148 54 28 0 2 55 437 490 2.13e-11 59.3
MsG0380017730.01.T01 AT4G17880 43.590 78 33 2 2 68 420 497 2.32e-11 59.3
MsG0380017730.01.T01 AT5G46760 48.148 54 28 0 2 55 419 472 3.82e-11 58.5
MsG0380017730.01.T01 AT5G41315 35.897 78 47 1 3 77 446 523 6.69e-11 57.8
MsG0380017730.01.T01 AT5G41315 35.897 78 47 1 3 77 454 531 7.53e-11 57.8
MsG0380017730.01.T01 AT5G41315 35.897 78 47 1 3 77 451 528 8.13e-11 57.8
MsG0380017730.01.T01 AT5G46830 47.541 61 29 1 2 59 347 407 8.92e-11 57.4

Find 15 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTACCTACCTTGCTTATCTT+AGG 0.245588 3:+100133737 None:intergenic
GCAACAGGAATTGGAGAAAA+AGG 0.270835 3:-100132835 MsG0380017730.01.T01:intron
ATACCCTGCACTTCTGAATA+AGG 0.328974 3:+100132782 None:intergenic
TCTCTTTCGTCTTCTCCTTT+CGG 0.335778 3:+100133796 None:intergenic
TACCTACCTTGCTTATCTTA+GGG 0.413903 3:+100133738 None:intergenic
CAAGCCTTATTCAGAAGTGC+AGG 0.468092 3:-100132786 MsG0380017730.01.T01:CDS
ATGGACAGAACAGCTATACT+TGG 0.546390 3:-100133621 MsG0380017730.01.T01:CDS
TTATTCAGAAGTGCAGGGTA+TGG 0.549665 3:-100132780 MsG0380017730.01.T01:CDS
TTCAGAAGTGCAGGGTATGG+TGG 0.556553 3:-100132777 MsG0380017730.01.T01:CDS
TTTCTGATTAAGATTTCACT+TGG 0.582741 3:+100133495 None:intergenic
CGAAGAATCTAATGGCCGAA+AGG 0.611328 3:-100133811 None:intergenic
CTTAATCAGAAATTCTCCAA+AGG 0.629828 3:-100133484 MsG0380017730.01.T01:intron
AATTGTCCCTAAGATAAGCA+AGG 0.652852 3:-100133744 MsG0380017730.01.T01:intron
AAGCCTTATTCAGAAGTGCA+GGG 0.666202 3:-100132785 MsG0380017730.01.T01:CDS
TTGCTCGTAGCATTGAAAGA+CGG 0.697294 3:+100133765 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGATATATAAATTCATCAAA+AGG - Chr3:100133516-100133535 MsG0380017730.01.T01:CDS 15.0%
!!! AGTAGTATATTAGAATTTTT+TGG - Chr3:100133610-100133629 MsG0380017730.01.T01:CDS 15.0%
!! AACACAGATTAATTTCAAAA+GGG + Chr3:100133121-100133140 None:intergenic 20.0%
!! AAGTATCACTAACAAATATT+AGG + Chr3:100133471-100133490 None:intergenic 20.0%
!! ACACACAGAAATAAAATAAT+TGG + Chr3:100133151-100133170 None:intergenic 20.0%
!! ATATATTGTCTTTCTTAGTT+AGG - Chr3:100133680-100133699 MsG0380017730.01.T01:intron 20.0%
!! GAAAGACAATATATACTATT+AGG + Chr3:100133674-100133693 None:intergenic 20.0%
!! TTCAACAAAACAAATTAGAT+CGG + Chr3:100132900-100132919 None:intergenic 20.0%
!!! ATCTAATTTGTTTTGTTGAA+TGG - Chr3:100132900-100132919 MsG0380017730.01.T01:intron 20.0%
! ACTTAATTCGACAGTTAAAA+AGG - Chr3:100133579-100133598 MsG0380017730.01.T01:CDS 25.0%
! CAACACAGATTAATTTCAAA+AGG + Chr3:100133122-100133141 None:intergenic 25.0%
! CAATAAATATTTGTGACAGA+TGG - Chr3:100132924-100132943 MsG0380017730.01.T01:intron 25.0%
! TTAGTTACTTAATACTTCCT+CGG - Chr3:100133369-100133388 MsG0380017730.01.T01:intron 25.0%
! TTGAAATTAATCTGTGTTGA+AGG - Chr3:100133123-100133142 MsG0380017730.01.T01:intron 25.0%
! TTTCTGATTAAGATTTCACT+TGG + Chr3:100133072-100133091 None:intergenic 25.0%
!!! AATTGTTACTTTGTAGTTTG+AGG - Chr3:100133185-100133204 MsG0380017730.01.T01:intron 25.0%
ATAACACATTGTTGAATCTC+AGG + Chr3:100133301-100133320 None:intergenic 30.0%
CGAATTAAGTCATTTACACA+TGG + Chr3:100133569-100133588 None:intergenic 30.0%
CTTAATCAGAAATTCTCCAA+AGG - Chr3:100133080-100133099 MsG0380017730.01.T01:intron 30.0%
TTTCAATGATTTCACAATGC+AGG - Chr3:100133327-100133346 MsG0380017730.01.T01:intron 30.0%
!! TTTCTTGTTTGTTATGCAAC+AGG - Chr3:100133714-100133733 MsG0380017730.01.T01:intron 30.0%
AATTGTCCCTAAGATAAGCA+AGG - Chr3:100132820-100132839 MsG0380017730.01.T01:CDS 35.0%
CGACATTTAAAAAGTAACCG+AGG + Chr3:100133389-100133408 None:intergenic 35.0%
TACCTACCTTGCTTATCTTA+GGG + Chr3:100132829-100132848 None:intergenic 35.0%
! GTCAGTGAATTTTGTACCTT+TGG + Chr3:100133099-100133118 None:intergenic 35.0%
!! GTAAACACATTGGAAGCATT+AGG - Chr3:100133275-100133294 MsG0380017730.01.T01:intron 35.0%
!! GTTTGTTATGCAACAGGAAT+TGG - Chr3:100133720-100133739 MsG0380017730.01.T01:intron 35.0%
AAGGGTTGAAATTGTTGTGC+AGG - Chr3:100133227-100133246 MsG0380017730.01.T01:intron 40.0%
AGGGTTGAAATTGTTGTGCA+GGG - Chr3:100133228-100133247 MsG0380017730.01.T01:intron 40.0%
ATACCCTGCACTTCTGAATA+AGG + Chr3:100133785-100133804 None:intergenic 40.0%
ATGGACAGAACAGCTATACT+TGG - Chr3:100132943-100132962 MsG0380017730.01.T01:intron 40.0%
GCAACAGGAATTGGAGAAAA+AGG - Chr3:100133729-100133748 MsG0380017730.01.T01:intron 40.0%
GCTATCCACAGTAAACACAT+TGG - Chr3:100133265-100133284 MsG0380017730.01.T01:intron 40.0%
GTACCTACCTTGCTTATCTT+AGG + Chr3:100132830-100132849 None:intergenic 40.0%
TCTCTTTCGTCTTCTCCTTT+CGG + Chr3:100132771-100132790 None:intergenic 40.0%
TGCTTCCAATGTGTTTACTG+TGG + Chr3:100133273-100133292 None:intergenic 40.0%
! CTTTGTAGTTTGAGGTAGAG+AGG - Chr3:100133193-100133212 MsG0380017730.01.T01:intron 40.0%
! TTATTCAGAAGTGCAGGGTA+TGG - Chr3:100133784-100133803 MsG0380017730.01.T01:CDS 40.0%
! TTGCTCGTAGCATTGAAAGA+CGG + Chr3:100132802-100132821 None:intergenic 40.0%
!! AAGCCTTATTCAGAAGTGCA+GGG - Chr3:100133779-100133798 MsG0380017730.01.T01:CDS 40.0%
CGACAGTTAAAAAGGAGCGA+GGG - Chr3:100133587-100133606 MsG0380017730.01.T01:CDS 45.0%
GTCCCTAAGATAAGCAAGGT+AGG - Chr3:100132824-100132843 MsG0380017730.01.T01:CDS 45.0%
TCGACAGTTAAAAAGGAGCG+AGG - Chr3:100133586-100133605 MsG0380017730.01.T01:CDS 45.0%
!! CAAGCCTTATTCAGAAGTGC+AGG - Chr3:100133778-100133797 MsG0380017730.01.T01:CDS 45.0%
AATTGTTGTGCAGGGAAGCC+AGG - Chr3:100133236-100133255 MsG0380017730.01.T01:intron 50.0%
ACTGTGGATAGCAACAACCC+TGG + Chr3:100133257-100133276 None:intergenic 50.0%
ATTGTTGTGCAGGGAAGCCA+GGG - Chr3:100133237-100133256 MsG0380017730.01.T01:intron 50.0%
CACAATGCAGGCTTCTTGCT+CGG - Chr3:100133339-100133358 MsG0380017730.01.T01:intron 50.0%
! TTCAGAAGTGCAGGGTATGG+TGG - Chr3:100133787-100133806 MsG0380017730.01.T01:CDS 50.0%
! TTTGAGGTAGAGAGGAGTGC+AGG - Chr3:100133201-100133220 MsG0380017730.01.T01:intron 50.0%
AATGCAGGCTTCTTGCTCGG+AGG - Chr3:100133342-100133361 MsG0380017730.01.T01:intron 55.0%
! AGAGAGGAGTGCAGGCACAA+GGG - Chr3:100133209-100133228 MsG0380017730.01.T01:intron 55.0%
! TAGAGAGGAGTGCAGGCACA+AGG - Chr3:100133208-100133227 MsG0380017730.01.T01:intron 55.0%
Chromosome Type Strat End Strand Name
Chr3 gene 100132764 100133822 100132764 ID=MsG0380017730.01;Name=MsG0380017730.01
Chr3 mRNA 100132764 100133822 100132764 ID=MsG0380017730.01.T01;Parent=MsG0380017730.01;Name=MsG0380017730.01.T01;_AED=0.43;_eAED=0.44;_QI=0|0|0|1|0.5|0.66|3|0|107
Chr3 exon 100133745 100133822 100133745 ID=MsG0380017730.01.T01:exon:17923;Parent=MsG0380017730.01.T01
Chr3 exon 100133485 100133643 100133485 ID=MsG0380017730.01.T01:exon:17922;Parent=MsG0380017730.01.T01
Chr3 exon 100132764 100132850 100132764 ID=MsG0380017730.01.T01:exon:17921;Parent=MsG0380017730.01.T01
Chr3 CDS 100133745 100133822 100133745 ID=MsG0380017730.01.T01:cds;Parent=MsG0380017730.01.T01
Chr3 CDS 100133485 100133643 100133485 ID=MsG0380017730.01.T01:cds;Parent=MsG0380017730.01.T01
Chr3 CDS 100132764 100132850 100132764 ID=MsG0380017730.01.T01:cds;Parent=MsG0380017730.01.T01
Gene Sequence

>MsG0380017730.01.T01

ATGGCCGAAAGGAGAAGACGAAAGAGATTAAATGACCGTCTTTCAATGCTACGAGCAATTGTCCCTAAGATAAGCAAGATGGACAGAACAGCTATACTTGGAGATACTATTGATTACATGAAAGAGCTTTTAGAGAAAATCAACAATCTGCAACAAGAAATTGAACTAGATTCAAATATGACAAGCATTGTCAAAGATGTCAAACCAAGTGAAATCTTAATCAGAAATTCTCCAAAGGAATTGGAGAAAAAGGCAATGCTAAGTTCTGAAGATATTAAGCAAGCCTTATTCAGAAGTGCAGGGTATGGTGGAAGATGTTTGTGA

Protein sequence

>MsG0380017730.01.T01

MAERRRRKRLNDRLSMLRAIVPKISKMDRTAILGDTIDYMKELLEKINNLQQEIELDSNMTSIVKDVKPSEILIRNSPKELEKKAMLSSEDIKQALFRSAGYGGRCL*