AlfalfaGEDB | MsG0280010663.01

M. sativa cultivar ZhongmuNo.1 / MsG0280010663.01

KEGG: -
GO: -
PFAM: PF12609

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010663.01.T01	XP_013465014.1	93.407	91	6	0	1	91	1	91	3.22E-56	178

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010663.01.T01	A0A072VAI4	93.407	91	6	0	1	91	1	91	1.54e-56	178

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048595.01	MsG0280010663.01	0.803980	2.690389e-49	1.371016e-46
MsG0180003430.01	MsG0280010663.01	0.827842	1.251571e-54	1.222529e-51
MsG0180005028.01	MsG0280010663.01	0.828916	6.890842e-55	6.944741e-52
MsG0180005984.01	MsG0280010663.01	0.809362	1.964283e-50	1.151010e-47
MsG0280010663.01	MsG0280010666.01	0.897844	9.477175e-77	1.090603e-72
MsG0280010663.01	MsG0280010667.01	0.860317	2.246835e-63	6.115940e-60
MsG0280010663.01	MsG0280010669.01	0.893371	6.692601e-75	6.357330e-71
MsG0280010663.01	MsG0280010670.01	0.849981	2.279969e-60	4.399644e-57
MsG0280010663.01	MsG0280010671.01	0.866377	2.980567e-65	1.002365e-61
MsG0280010663.01	MsG0280010673.01	0.801506	8.719820e-49	4.171453e-46
MsG0280010663.01	MsG0380017076.01	0.821021	5.039223e-53	4.052823e-50
MsG0280010663.01	MsG0480019017.01	0.815072	1.114862e-51	7.609509e-49
MsG0280010663.01	MsG0480023360.01	0.838467	2.826072e-57	3.788684e-54
MsG0280010663.01	MsG0680030732.01	0.822262	2.602226e-53	2.167186e-50

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010663.01.T01	MTR_2g089930	93.407	91	6	0	1	91	1	91	3.90e-60	178
MsG0280010663.01.T01	MTR_2g089945	92.308	91	7	0	1	91	1	91	3.10e-59	176
MsG0280010663.01.T01	MTR_2g089865	89.655	87	9	0	1	87	1	87	1.38e-48	156
MsG0280010663.01.T01	MTR_4g045990	74.118	85	19	2	9	91	6	89	2.20e-37	120
MsG0280010663.01.T01	MTR_2g089970	67.442	86	23	3	9	91	6	89	1.01e-34	114
MsG0280010663.01.T01	MTR_2g089990	63.953	86	26	3	9	91	6	89	2.91e-32	108
MsG0280010663.01.T01	MTR_2g089950	66.667	81	21	3	11	91	10	84	9.86e-32	106
MsG0280010663.01.T01	MTR_2g089925	66.667	81	21	3	11	91	10	84	1.67e-31	105
MsG0280010663.01.T01	MTR_2g089905	61.957	92	29	3	5	91	2	92	6.00e-31	104
MsG0280010663.01.T01	MTR_2g089940	60.440	91	31	2	5	91	2	91	5.51e-30	102
MsG0280010663.01.T01	MTR_2g089985	60.440	91	31	2	5	91	2	91	5.51e-30	102
MsG0280010663.01.T01	MTR_2g089960	60.440	91	31	2	5	91	2	91	5.51e-30	102
MsG0280010663.01.T01	MTR_2g089965	57.292	96	31	2	5	91	2	96	2.93e-28	98.2
MsG0280010663.01.T01	MTR_2g481160	54.651	86	36	1	5	90	2	84	2.42e-26	93.2
MsG0280010663.01.T01	MTR_2g081155	56.322	87	31	3	5	90	2	82	3.39e-26	92.8
MsG0280010663.01.T01	MTR_2g481150	52.326	86	38	1	5	90	2	84	1.28e-25	91.3
MsG0280010663.01.T01	MTR_2g089835	56.818	88	34	4	6	91	3	88	2.69e-23	85.5
MsG0280010663.01.T01	MTR_2g011690	47.297	74	33	3	21	91	19	89	1.70e-11	55.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010663.01.T01	AT4G10265	67.778	90	21	2	1	90	1	82	9.09e-37	119
MsG0280010663.01.T01	AT4G10270	66.292	89	28	1	5	91	2	90	1.36e-35	116

Find 23 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATTTCTTTCTACCAAATTTA+AGG	0.083061	2:+73099202	MsG0280010663.01.T01:CDS
CTTCTTCTCATCCTTAAATT+TGG	0.217937	2:-73099213	None:intergenic
CAAGGACTCCTCTGCTTGTT+TGG	0.238006	2:-73099237	None:intergenic
CTATGTACAAATTAATCAAT+TGG	0.386618	2:-73099291	None:intergenic
TGTTATGTATTTAAGCTGCT+GGG	0.439197	2:+73099265	MsG0280010663.01.T01:CDS
CTGTTATGTATTTAAGCTGC+TGG	0.447401	2:+73099264	MsG0280010663.01.T01:CDS
GGCTGCAAGTGTTGGAGTTG+TGG	0.471925	2:+73099061	MsG0280010663.01.T01:CDS
TATGTACAAATTAATCAATT+GGG	0.486617	2:-73099290	None:intergenic
CAAATTTAAGGATGAGAAGA+AGG	0.489333	2:+73099214	MsG0280010663.01.T01:CDS
AGCAACAAGCAAAGCATGGA+TGG	0.538950	2:+73099037	MsG0280010663.01.T01:CDS
CTTGAGACATTGATCTAACA+CGG	0.543910	2:-73099157	None:intergenic
ATAGTTCCACCTACAGATAC+CGG	0.568375	2:-73099102	None:intergenic
CAACAGCAACAAGCAAAGCA+TGG	0.569709	2:+73099033	MsG0280010663.01.T01:CDS
GTTATGTATTTAAGCTGCTG+GGG	0.572155	2:+73099266	MsG0280010663.01.T01:CDS
TGGATGGTGGCTGCAAGTGT+TGG	0.573709	2:+73099053	MsG0280010663.01.T01:CDS
TGCAAGTGTTGGAGTTGTGG+AGG	0.587512	2:+73099064	MsG0280010663.01.T01:CDS
TAAATACATAACAGTCCTCA+AGG	0.598210	2:-73099255	None:intergenic
GAAGAAGGCCAAACAAGCAG+AGG	0.609690	2:+73099229	MsG0280010663.01.T01:CDS
AAGATCAAGCCGGTATCTGT+AGG	0.628254	2:+73099093	MsG0280010663.01.T01:CDS
AACAAGCAGAGGAGTCCTTG+AGG	0.628944	2:+73099240	MsG0280010663.01.T01:CDS
ATCAAGCCGGTATCTGTAGG+TGG	0.630665	2:+73099096	MsG0280010663.01.T01:CDS
AACAAGCAAAGCATGGATGG+TGG	0.650577	2:+73099040	MsG0280010663.01.T01:CDS
GAGGCTTTGAAAGATCAAGC+CGG	0.680929	2:+73099083	MsG0280010663.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTTCTTTCTACCAAATTTA+AGG	+	Chr2:73099202-73099221	MsG0280010663.01.T01:CDS	20.0%
	CAAATTTAAGGATGAGAAGA+AGG	+	Chr2:73099214-73099233	MsG0280010663.01.T01:CDS	30.0%
	CTTCTTCTCATCCTTAAATT+TGG	-	Chr2:73099216-73099235	None:intergenic	30.0%
	TAAATACATAACAGTCCTCA+AGG	-	Chr2:73099258-73099277	None:intergenic	30.0%
	TGTTATGTATTTAAGCTGCT+GGG	+	Chr2:73099265-73099284	MsG0280010663.01.T01:CDS	30.0%
!	TTGATCTAACACGGTTTTTA+AGG	-	Chr2:73099151-73099170	None:intergenic	30.0%
	CTGTTATGTATTTAAGCTGC+TGG	+	Chr2:73099264-73099283	MsG0280010663.01.T01:CDS	35.0%
	CTTGAGACATTGATCTAACA+CGG	-	Chr2:73099160-73099179	None:intergenic	35.0%
	GTTATGTATTTAAGCTGCTG+GGG	+	Chr2:73099266-73099285	MsG0280010663.01.T01:CDS	35.0%
	ATAGTTCCACCTACAGATAC+CGG	-	Chr2:73099105-73099124	None:intergenic	40.0%
	AACAAGCAAAGCATGGATGG+TGG	+	Chr2:73099040-73099059	MsG0280010663.01.T01:CDS	45.0%
	AAGATCAAGCCGGTATCTGT+AGG	+	Chr2:73099093-73099112	MsG0280010663.01.T01:CDS	45.0%
	AGCAACAAGCAAAGCATGGA+TGG	+	Chr2:73099037-73099056	MsG0280010663.01.T01:CDS	45.0%
	CAACAGCAACAAGCAAAGCA+TGG	+	Chr2:73099033-73099052	MsG0280010663.01.T01:CDS	45.0%
!!	GAGGCTTTGAAAGATCAAGC+CGG	+	Chr2:73099083-73099102	MsG0280010663.01.T01:CDS	45.0%
	AACAAGCAGAGGAGTCCTTG+AGG	+	Chr2:73099240-73099259	MsG0280010663.01.T01:CDS	50.0%
	ATCAAGCCGGTATCTGTAGG+TGG	+	Chr2:73099096-73099115	MsG0280010663.01.T01:CDS	50.0%
	CAAGGACTCCTCTGCTTGTT+TGG	-	Chr2:73099240-73099259	None:intergenic	50.0%
	GAAGAAGGCCAAACAAGCAG+AGG	+	Chr2:73099229-73099248	MsG0280010663.01.T01:CDS	50.0%
!!	TGCAAGTGTTGGAGTTGTGG+AGG	+	Chr2:73099064-73099083	MsG0280010663.01.T01:CDS	50.0%
!	TGGATGGTGGCTGCAAGTGT+TGG	+	Chr2:73099053-73099072	MsG0280010663.01.T01:CDS	55.0%
!!	GGCTGCAAGTGTTGGAGTTG+TGG	+	Chr2:73099061-73099080	MsG0280010663.01.T01:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	73099023	73099298	73099023	ID=MsG0280010663.01;Name=MsG0280010663.01
Chr2	mRNA	73099023	73099298	73099023	ID=MsG0280010663.01.T01;Parent=MsG0280010663.01;Name=MsG0280010663.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|91
Chr2	exon	73099023	73099298	73099023	ID=MsG0280010663.01.T01:exon:19616;Parent=MsG0280010663.01.T01
Chr2	CDS	73099023	73099298	73099023	ID=MsG0280010663.01.T01:cds;Parent=MsG0280010663.01.T01

Gene Sequence	>MsG0280010663.01.T01 ATGAGTAGTGCAACAGCAACAAGCAAAGCATGGATGGTGGCTGCAAGTGTTGGAGTTGTGGAGGCTTTGAAAGATCAAGCCGGTATCTGTAGGTGGAACTATGCTTTAAGACAAGCTCAACAACACCTTAAAAACCGTGTTAGATCAATGTCTCAAGCAAAGAATTTCTCTTCTTCTTCATTTCTTTCTACCAAATTTAAGGATGAGAAGAAGGCCAAACAAGCAGAGGAGTCCTTGAGGACTGTTATGTATTTAAGCTGCTGGGGTCCCAATTGA
Protein sequence	>MsG0280010663.01.T01 MSSATATSKAWMVAASVGVVEALKDQAGICRWNYALRQAQQHLKNRVRSMSQAKNFSSSSFLSTKFKDEKKAKQAEESLRTVMYLSCWGPN*