Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010671.01.T01 | XP_013465014.1 | 92.308 | 91 | 7 | 0 | 1 | 91 | 1 | 91 | 7.83E-56 | 177 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010671.01.T01 | A0A072VAI4 | 92.308 | 91 | 7 | 0 | 1 | 91 | 1 | 91 | 3.74e-56 | 177 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003430.01 | MsG0280010671.01 | 0.834997 | 2.168451e-56 | 2.616830e-53 |
| MsG0180005028.01 | MsG0280010671.01 | 0.817338 | 3.473181e-52 | 2.522428e-49 |
| MsG0180005514.01 | MsG0280010671.01 | 0.804315 | 2.291698e-49 | 1.177858e-46 |
| MsG0280010663.01 | MsG0280010671.01 | 0.866377 | 2.980567e-65 | 1.002365e-61 |
| MsG0280010666.01 | MsG0280010671.01 | 0.926431 | 4.733008e-91 | 2.231365e-86 |
| MsG0280010667.01 | MsG0280010671.01 | 0.809225 | 2.101628e-50 | 1.227001e-47 |
| MsG0280010669.01 | MsG0280010671.01 | 0.894900 | 1.594912e-75 | 1.617100e-71 |
| MsG0280010670.01 | MsG0280010671.01 | 0.808080 | 3.692224e-50 | 2.091297e-47 |
| MsG0280010671.01 | MsG0280010673.01 | 0.822772 | 1.980985e-53 | 1.673999e-50 |
| MsG0280010671.01 | MsG0380015543.01 | 0.809157 | 2.173824e-50 | 1.266806e-47 |
| MsG0280010671.01 | MsG0680030732.01 | 0.807492 | 4.922681e-50 | 2.745783e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010671.01.T01 | MTR_2g089930 | 92.308 | 91 | 7 | 0 | 1 | 91 | 1 | 91 | 9.48e-60 | 177 |
| MsG0280010671.01.T01 | MTR_2g089945 | 90.110 | 91 | 9 | 0 | 1 | 91 | 1 | 91 | 3.08e-58 | 173 |
| MsG0280010671.01.T01 | MTR_2g089865 | 87.356 | 87 | 11 | 0 | 1 | 87 | 1 | 87 | 1.08e-47 | 154 |
| MsG0280010671.01.T01 | MTR_4g045990 | 70.588 | 85 | 22 | 2 | 9 | 91 | 6 | 89 | 4.69e-36 | 117 |
| MsG0280010671.01.T01 | MTR_2g089970 | 62.222 | 90 | 29 | 3 | 5 | 91 | 2 | 89 | 5.81e-34 | 112 |
| MsG0280010671.01.T01 | MTR_2g089990 | 61.628 | 86 | 28 | 3 | 9 | 91 | 6 | 89 | 1.12e-31 | 106 |
| MsG0280010671.01.T01 | MTR_2g089950 | 63.415 | 82 | 24 | 3 | 10 | 91 | 9 | 84 | 2.70e-30 | 102 |
| MsG0280010671.01.T01 | MTR_2g089925 | 62.963 | 81 | 24 | 3 | 11 | 91 | 10 | 84 | 8.91e-30 | 101 |
| MsG0280010671.01.T01 | MTR_2g089905 | 58.696 | 92 | 32 | 3 | 5 | 91 | 2 | 92 | 1.10e-29 | 101 |
| MsG0280010671.01.T01 | MTR_2g089940 | 58.242 | 91 | 33 | 2 | 5 | 91 | 2 | 91 | 1.05e-28 | 99.4 |
| MsG0280010671.01.T01 | MTR_2g089985 | 58.242 | 91 | 33 | 2 | 5 | 91 | 2 | 91 | 1.05e-28 | 99.4 |
| MsG0280010671.01.T01 | MTR_2g089960 | 58.242 | 91 | 33 | 2 | 5 | 91 | 2 | 91 | 1.05e-28 | 99.4 |
| MsG0280010671.01.T01 | MTR_2g089965 | 55.208 | 96 | 33 | 2 | 5 | 91 | 2 | 96 | 5.69e-27 | 95.1 |
| MsG0280010671.01.T01 | MTR_2g481160 | 54.651 | 86 | 36 | 1 | 5 | 90 | 2 | 84 | 1.92e-26 | 93.6 |
| MsG0280010671.01.T01 | MTR_2g081155 | 56.322 | 87 | 31 | 3 | 5 | 90 | 2 | 82 | 2.97e-26 | 92.8 |
| MsG0280010671.01.T01 | MTR_2g481150 | 52.326 | 86 | 38 | 1 | 5 | 90 | 2 | 84 | 1.04e-25 | 91.7 |
| MsG0280010671.01.T01 | MTR_2g089835 | 54.545 | 88 | 36 | 4 | 6 | 91 | 3 | 88 | 7.38e-22 | 81.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010671.01.T01 | AT4G10265 | 62.069 | 87 | 28 | 1 | 5 | 91 | 2 | 83 | 6.00e-35 | 114 |
| MsG0280010671.01.T01 | AT4G10270 | 64.045 | 89 | 30 | 1 | 5 | 91 | 2 | 90 | 2.22e-34 | 113 |
Find 23 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTATTGCTAGCAAATTTA+AGG | 0.110379 | 2:+73172726 | MsG0280010671.01.T01:CDS |
| CTATGTACAAATTAATCAAT+TGG | 0.391707 | 2:-73172815 | None:intergenic |
| GGATGAGAAGAAGGCAAAAC+AGG | 0.400035 | 2:+73172747 | MsG0280010671.01.T01:CDS |
| CTGTCATGTACTTGAGCTGC+TGG | 0.406277 | 2:+73172788 | MsG0280010671.01.T01:CDS |
| TTAAATTTGCTAGCAATAAG+TGG | 0.408213 | 2:-73172724 | None:intergenic |
| TGTCATGTACTTGAGCTGCT+GGG | 0.470465 | 2:+73172789 | MsG0280010671.01.T01:CDS |
| GGCTGCAAGTGTTGGAGTTG+TGG | 0.471925 | 2:+73172585 | MsG0280010671.01.T01:CDS |
| TATGTACAAATTAATCAATT+GGG | 0.478187 | 2:-73172814 | None:intergenic |
| CAAATTTAAGGATGAGAAGA+AGG | 0.496478 | 2:+73172738 | MsG0280010671.01.T01:CDS |
| AGCAACAAGCAAAGCATGGA+TGG | 0.538950 | 2:+73172561 | MsG0280010671.01.T01:CDS |
| CAAGTACATGACAGTCCTCA+AGG | 0.563012 | 2:-73172779 | None:intergenic |
| GTCATGTACTTGAGCTGCTG+GGG | 0.565171 | 2:+73172790 | MsG0280010671.01.T01:CDS |
| ATAGTTCCACCTACAGATAC+CGG | 0.568375 | 2:-73172626 | None:intergenic |
| CAACAGCAACAAGCAAAGCA+TGG | 0.569299 | 2:+73172557 | MsG0280010671.01.T01:CDS |
| CTTGAGACATTGATTTAACA+CGG | 0.573162 | 2:-73172681 | None:intergenic |
| TGGATGGTGGCTGCAAGTGT+TGG | 0.573709 | 2:+73172577 | MsG0280010671.01.T01:CDS |
| GAAGAAGGCAAAACAGGCAG+AGG | 0.577515 | 2:+73172753 | MsG0280010671.01.T01:CDS |
| TGCAAGTGTTGGAGTTGTGG+AGG | 0.587512 | 2:+73172588 | MsG0280010671.01.T01:CDS |
| AAGATCAAGCCGGTATCTGT+AGG | 0.628254 | 2:+73172617 | MsG0280010671.01.T01:CDS |
| ATCAAGCCGGTATCTGTAGG+TGG | 0.630665 | 2:+73172620 | MsG0280010671.01.T01:CDS |
| AACAAGCAAAGCATGGATGG+TGG | 0.650577 | 2:+73172564 | MsG0280010671.01.T01:CDS |
| GAGGCTTTGAAAGATCAAGC+CGG | 0.680929 | 2:+73172607 | MsG0280010671.01.T01:CDS |
| AACAGGCAGAGGAATCCTTG+AGG | 0.700969 | 2:+73172764 | MsG0280010671.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACTTATTGCTAGCAAATTTA+AGG | + | Chr2:73172726-73172745 | MsG0280010671.01.T01:CDS | 25.0% |
| !! | TTGATTTAACACGGTTTTTA+AGG | - | Chr2:73172675-73172694 | None:intergenic | 25.0% |
| !!! | TTAAATTTGCTAGCAATAAG+TGG | - | Chr2:73172727-73172746 | None:intergenic | 25.0% |
| CAAATTTAAGGATGAGAAGA+AGG | + | Chr2:73172738-73172757 | MsG0280010671.01.T01:CDS | 30.0% | |
| !! | CTTGAGACATTGATTTAACA+CGG | - | Chr2:73172684-73172703 | None:intergenic | 30.0% |
| ATAGTTCCACCTACAGATAC+CGG | - | Chr2:73172629-73172648 | None:intergenic | 40.0% | |
| AACAAGCAAAGCATGGATGG+TGG | + | Chr2:73172564-73172583 | MsG0280010671.01.T01:CDS | 45.0% | |
| AAGATCAAGCCGGTATCTGT+AGG | + | Chr2:73172617-73172636 | MsG0280010671.01.T01:CDS | 45.0% | |
| AGCAACAAGCAAAGCATGGA+TGG | + | Chr2:73172561-73172580 | MsG0280010671.01.T01:CDS | 45.0% | |
| CAACAGCAACAAGCAAAGCA+TGG | + | Chr2:73172557-73172576 | MsG0280010671.01.T01:CDS | 45.0% | |
| CAAGTACATGACAGTCCTCA+AGG | - | Chr2:73172782-73172801 | None:intergenic | 45.0% | |
| GGATGAGAAGAAGGCAAAAC+AGG | + | Chr2:73172747-73172766 | MsG0280010671.01.T01:CDS | 45.0% | |
| TGTCATGTACTTGAGCTGCT+GGG | + | Chr2:73172789-73172808 | MsG0280010671.01.T01:CDS | 45.0% | |
| !! | GAGGCTTTGAAAGATCAAGC+CGG | + | Chr2:73172607-73172626 | MsG0280010671.01.T01:CDS | 45.0% |
| AACAGGCAGAGGAATCCTTG+AGG | + | Chr2:73172764-73172783 | MsG0280010671.01.T01:CDS | 50.0% | |
| ATCAAGCCGGTATCTGTAGG+TGG | + | Chr2:73172620-73172639 | MsG0280010671.01.T01:CDS | 50.0% | |
| CTGTCATGTACTTGAGCTGC+TGG | + | Chr2:73172788-73172807 | MsG0280010671.01.T01:CDS | 50.0% | |
| GAAGAAGGCAAAACAGGCAG+AGG | + | Chr2:73172753-73172772 | MsG0280010671.01.T01:CDS | 50.0% | |
| GTCATGTACTTGAGCTGCTG+GGG | + | Chr2:73172790-73172809 | MsG0280010671.01.T01:CDS | 50.0% | |
| !! | TGCAAGTGTTGGAGTTGTGG+AGG | + | Chr2:73172588-73172607 | MsG0280010671.01.T01:CDS | 50.0% |
| ! | TGGATGGTGGCTGCAAGTGT+TGG | + | Chr2:73172577-73172596 | MsG0280010671.01.T01:CDS | 55.0% |
| !! | GGCTGCAAGTGTTGGAGTTG+TGG | + | Chr2:73172585-73172604 | MsG0280010671.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 73172547 | 73172822 | 73172547 | ID=MsG0280010671.01;Name=MsG0280010671.01 |
| Chr2 | mRNA | 73172547 | 73172822 | 73172547 | ID=MsG0280010671.01.T01;Parent=MsG0280010671.01;Name=MsG0280010671.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|91 |
| Chr2 | exon | 73172547 | 73172822 | 73172547 | ID=MsG0280010671.01.T01:exon:19629;Parent=MsG0280010671.01.T01 |
| Chr2 | CDS | 73172547 | 73172822 | 73172547 | ID=MsG0280010671.01.T01:cds;Parent=MsG0280010671.01.T01 |
| Gene Sequence |
| Protein sequence |
