Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010666.01.T01 | XP_024633071.1 | 93.407 | 91 | 6 | 0 | 1 | 91 | 1 | 91 | 5.82E-56 | 177 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010666.01.T01 | I3SV61 | 93.407 | 91 | 6 | 0 | 1 | 91 | 1 | 91 | 2.78e-56 | 177 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003430.01 | MsG0280010666.01 | 0.864968 | 8.300810e-65 | 2.655673e-61 |
| MsG0180005028.01 | MsG0280010666.01 | 0.843469 | 1.374551e-58 | 2.152751e-55 |
| MsG0180005984.01 | MsG0280010666.01 | 0.801932 | 7.128930e-49 | 3.447349e-46 |
| MsG0280010663.01 | MsG0280010666.01 | 0.897844 | 9.477175e-77 | 1.090603e-72 |
| MsG0280010666.01 | MsG0280010667.01 | 0.841914 | 3.558075e-58 | 5.306348e-55 |
| MsG0280010666.01 | MsG0280010669.01 | 0.931479 | 3.554135e-94 | 2.245955e-89 |
| MsG0280010666.01 | MsG0280010670.01 | 0.833618 | 4.810059e-56 | 5.568971e-53 |
| MsG0280010666.01 | MsG0280010671.01 | 0.926431 | 4.733008e-91 | 2.231365e-86 |
| MsG0280010666.01 | MsG0280010673.01 | 0.848070 | 7.746016e-60 | 1.404487e-56 |
| MsG0280010666.01 | MsG0380017730.01 | 0.809808 | 1.575981e-50 | 9.344295e-48 |
| MsG0280010666.01 | MsG0680030732.01 | 0.807790 | 4.254663e-50 | 2.391626e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010666.01.T01 | MTR_2g089930 | 91.209 | 91 | 8 | 0 | 1 | 91 | 1 | 91 | 1.79e-59 | 176 |
| MsG0280010666.01.T01 | MTR_2g089945 | 92.308 | 91 | 7 | 0 | 1 | 91 | 1 | 91 | 2.38e-59 | 176 |
| MsG0280010666.01.T01 | MTR_2g089865 | 89.655 | 87 | 9 | 0 | 1 | 87 | 1 | 87 | 1.77e-48 | 155 |
| MsG0280010666.01.T01 | MTR_4g045990 | 74.118 | 85 | 19 | 2 | 9 | 91 | 6 | 89 | 1.77e-37 | 121 |
| MsG0280010666.01.T01 | MTR_2g089970 | 66.279 | 86 | 24 | 3 | 9 | 91 | 6 | 89 | 3.83e-34 | 112 |
| MsG0280010666.01.T01 | MTR_2g089950 | 67.901 | 81 | 20 | 3 | 11 | 91 | 10 | 84 | 3.88e-32 | 107 |
| MsG0280010666.01.T01 | MTR_2g089990 | 64.286 | 84 | 25 | 3 | 11 | 91 | 8 | 89 | 9.39e-32 | 106 |
| MsG0280010666.01.T01 | MTR_2g089925 | 64.198 | 81 | 23 | 3 | 11 | 91 | 10 | 84 | 1.60e-30 | 103 |
| MsG0280010666.01.T01 | MTR_2g089905 | 63.333 | 90 | 27 | 3 | 7 | 91 | 4 | 92 | 3.00e-30 | 103 |
| MsG0280010666.01.T01 | MTR_2g089940 | 64.045 | 89 | 27 | 2 | 7 | 91 | 4 | 91 | 1.49e-29 | 101 |
| MsG0280010666.01.T01 | MTR_2g089985 | 64.045 | 89 | 27 | 2 | 7 | 91 | 4 | 91 | 1.49e-29 | 101 |
| MsG0280010666.01.T01 | MTR_2g089960 | 64.045 | 89 | 27 | 2 | 7 | 91 | 4 | 91 | 1.49e-29 | 101 |
| MsG0280010666.01.T01 | MTR_2g089965 | 57.447 | 94 | 30 | 2 | 7 | 91 | 4 | 96 | 9.45e-28 | 97.1 |
| MsG0280010666.01.T01 | MTR_2g481160 | 55.952 | 84 | 34 | 1 | 8 | 91 | 5 | 85 | 1.07e-25 | 91.7 |
| MsG0280010666.01.T01 | MTR_2g081155 | 54.023 | 87 | 33 | 3 | 5 | 90 | 2 | 82 | 1.79e-25 | 90.9 |
| MsG0280010666.01.T01 | MTR_2g481150 | 54.217 | 83 | 35 | 1 | 8 | 90 | 5 | 84 | 4.35e-25 | 90.1 |
| MsG0280010666.01.T01 | MTR_2g089835 | 52.326 | 86 | 39 | 2 | 7 | 91 | 4 | 88 | 7.35e-23 | 84.3 |
| MsG0280010666.01.T01 | MTR_2g011690 | 46.053 | 76 | 35 | 3 | 19 | 91 | 17 | 89 | 2.09e-11 | 55.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010666.01.T01 | AT4G10265 | 67.857 | 84 | 22 | 1 | 8 | 91 | 5 | 83 | 8.70e-37 | 119 |
| MsG0280010666.01.T01 | AT4G10270 | 68.966 | 87 | 25 | 1 | 7 | 91 | 4 | 90 | 1.31e-35 | 116 |
Find 22 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTTGTTGCTACCAAATTTA+AGG | 0.083204 | 2:+73120240 | MsG0280010666.01.T01:CDS |
| CTTCTTGTCATCCTTAAATT+TGG | 0.215497 | 2:-73120251 | None:intergenic |
| CAAAGACTCCTCTGCCTGTT+TGG | 0.289223 | 2:-73120275 | None:intergenic |
| CACTGTACAATTGAATCAAT+TGG | 0.325370 | 2:-73120329 | None:intergenic |
| CTGTCATGTACTTGAGCTGC+TGG | 0.406277 | 2:+73120302 | MsG0280010666.01.T01:CDS |
| AAGATCAAGCTGGTATTTGT+AGG | 0.423985 | 2:+73120131 | MsG0280010666.01.T01:CDS |
| GGATGACAAGAAGGCCAAAC+AGG | 0.439731 | 2:+73120261 | MsG0280010666.01.T01:CDS |
| AACAGGCAGAGGAGTCTTTG+AGG | 0.455186 | 2:+73120278 | MsG0280010666.01.T01:CDS |
| TGTCATGTACTTGAGCTGCT+GGG | 0.470465 | 2:+73120303 | MsG0280010666.01.T01:CDS |
| GGCTGCAAGTGTTGGAGTTG+TGG | 0.471925 | 2:+73120099 | MsG0280010666.01.T01:CDS |
| ACTGTACAATTGAATCAATT+GGG | 0.478022 | 2:-73120328 | None:intergenic |
| CAAGAAGGCCAAACAGGCAG+AGG | 0.502891 | 2:+73120267 | MsG0280010666.01.T01:CDS |
| CAAATTTAAGGATGACAAGA+AGG | 0.525172 | 2:+73120252 | MsG0280010666.01.T01:CDS |
| CTTGAGAAATTGATCTAACA+CGG | 0.546721 | 2:-73120195 | None:intergenic |
| AGTAACAAGCAAAGCATGGA+TGG | 0.564370 | 2:+73120075 | MsG0280010666.01.T01:CDS |
| GTCATGTACTTGAGCTGCTG+GGG | 0.565171 | 2:+73120304 | MsG0280010666.01.T01:CDS |
| TGGATGGTGGCTGCAAGTGT+TGG | 0.573709 | 2:+73120091 | MsG0280010666.01.T01:CDS |
| CAGCAGTAACAAGCAAAGCA+TGG | 0.579805 | 2:+73120071 | MsG0280010666.01.T01:CDS |
| TGCAAGTGTTGGAGTTGTGG+AGG | 0.602761 | 2:+73120102 | MsG0280010666.01.T01:CDS |
| ATCAAGCTGGTATTTGTAGG+TGG | 0.610975 | 2:+73120134 | MsG0280010666.01.T01:CDS |
| AACAAGCAAAGCATGGATGG+TGG | 0.655314 | 2:+73120078 | MsG0280010666.01.T01:CDS |
| GAGGCATTGAAAGATCAAGC+TGG | 0.657595 | 2:+73120121 | MsG0280010666.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTGTTGCTACCAAATTTA+AGG | + | Chr2:73120240-73120259 | MsG0280010666.01.T01:CDS | 25.0% |
| CAAATTTAAGGATGACAAGA+AGG | + | Chr2:73120252-73120271 | MsG0280010666.01.T01:CDS | 30.0% | |
| CTTCTTGTCATCCTTAAATT+TGG | - | Chr2:73120254-73120273 | None:intergenic | 30.0% | |
| CTTGAGAAATTGATCTAACA+CGG | - | Chr2:73120198-73120217 | None:intergenic | 30.0% | |
| ! | TTGATCTAACACGGTTTTTA+AGG | - | Chr2:73120189-73120208 | None:intergenic | 30.0% |
| AAGATCAAGCTGGTATTTGT+AGG | + | Chr2:73120131-73120150 | MsG0280010666.01.T01:CDS | 35.0% | |
| AGTAACAAGCAAAGCATGGA+TGG | + | Chr2:73120075-73120094 | MsG0280010666.01.T01:CDS | 40.0% | |
| ATCAAGCTGGTATTTGTAGG+TGG | + | Chr2:73120134-73120153 | MsG0280010666.01.T01:CDS | 40.0% | |
| AACAAGCAAAGCATGGATGG+TGG | + | Chr2:73120078-73120097 | MsG0280010666.01.T01:CDS | 45.0% | |
| CAGCAGTAACAAGCAAAGCA+TGG | + | Chr2:73120071-73120090 | MsG0280010666.01.T01:CDS | 45.0% | |
| TGTCATGTACTTGAGCTGCT+GGG | + | Chr2:73120303-73120322 | MsG0280010666.01.T01:CDS | 45.0% | |
| !! | GAGGCATTGAAAGATCAAGC+TGG | + | Chr2:73120121-73120140 | MsG0280010666.01.T01:CDS | 45.0% |
| AACAGGCAGAGGAGTCTTTG+AGG | + | Chr2:73120278-73120297 | MsG0280010666.01.T01:CDS | 50.0% | |
| CAAAGACTCCTCTGCCTGTT+TGG | - | Chr2:73120278-73120297 | None:intergenic | 50.0% | |
| CTGTCATGTACTTGAGCTGC+TGG | + | Chr2:73120302-73120321 | MsG0280010666.01.T01:CDS | 50.0% | |
| GGATGACAAGAAGGCCAAAC+AGG | + | Chr2:73120261-73120280 | MsG0280010666.01.T01:CDS | 50.0% | |
| GTCATGTACTTGAGCTGCTG+GGG | + | Chr2:73120304-73120323 | MsG0280010666.01.T01:CDS | 50.0% | |
| !! | TGCAAGTGTTGGAGTTGTGG+AGG | + | Chr2:73120102-73120121 | MsG0280010666.01.T01:CDS | 50.0% |
| CAAGAAGGCCAAACAGGCAG+AGG | + | Chr2:73120267-73120286 | MsG0280010666.01.T01:CDS | 55.0% | |
| ! | TGGATGGTGGCTGCAAGTGT+TGG | + | Chr2:73120091-73120110 | MsG0280010666.01.T01:CDS | 55.0% |
| !! | GGCTGCAAGTGTTGGAGTTG+TGG | + | Chr2:73120099-73120118 | MsG0280010666.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 73120061 | 73120336 | 73120061 | ID=MsG0280010666.01;Name=MsG0280010666.01 |
| Chr2 | mRNA | 73120061 | 73120336 | 73120061 | ID=MsG0280010666.01.T01;Parent=MsG0280010666.01;Name=MsG0280010666.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|91 |
| Chr2 | exon | 73120061 | 73120336 | 73120061 | ID=MsG0280010666.01.T01:exon:34470;Parent=MsG0280010666.01.T01 |
| Chr2 | CDS | 73120061 | 73120336 | 73120061 | ID=MsG0280010666.01.T01:cds;Parent=MsG0280010666.01.T01 |
| Gene Sequence |
| Protein sequence |
