Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010670.01.T01 | XP_013465011.1 | 92.391 | 92 | 4 | 1 | 1 | 89 | 1 | 92 | 2.28E-47 | 156 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010670.01.T01 | A0A072VBQ8 | 92.391 | 92 | 4 | 1 | 1 | 89 | 1 | 92 | 1.09e-47 | 156 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003430.01 | MsG0280010670.01 | 0.820991 | 5.119905e-53 | 4.114256e-50 |
| MsG0180005984.01 | MsG0280010670.01 | 0.804774 | 1.837965e-49 | 9.559467e-47 |
| MsG0280010663.01 | MsG0280010670.01 | 0.849981 | 2.279969e-60 | 4.399644e-57 |
| MsG0280010666.01 | MsG0280010670.01 | 0.833618 | 4.810059e-56 | 5.568971e-53 |
| MsG0280010667.01 | MsG0280010670.01 | 0.917845 | 3.229526e-86 | 9.533306e-82 |
| MsG0280010669.01 | MsG0280010670.01 | 0.813802 | 2.128957e-51 | 1.404085e-48 |
| MsG0280010670.01 | MsG0280010671.01 | 0.808080 | 3.692224e-50 | 2.091297e-47 |
| MsG0280010670.01 | MsG0280010673.01 | 0.815105 | 1.096293e-51 | 7.489246e-49 |
| MsG0280010670.01 | MsG0380017730.01 | 0.802283 | 6.038374e-49 | 2.946334e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010670.01.T01 | MTR_2g089905 | 92.391 | 92 | 4 | 1 | 1 | 89 | 1 | 92 | 2.76e-51 | 156 |
| MsG0280010670.01.T01 | MTR_2g089940 | 87.912 | 91 | 9 | 1 | 1 | 89 | 1 | 91 | 4.19e-47 | 145 |
| MsG0280010670.01.T01 | MTR_2g089985 | 87.912 | 91 | 9 | 1 | 1 | 89 | 1 | 91 | 4.19e-47 | 145 |
| MsG0280010670.01.T01 | MTR_2g089960 | 87.912 | 91 | 9 | 1 | 1 | 89 | 1 | 91 | 4.19e-47 | 145 |
| MsG0280010670.01.T01 | MTR_2g089965 | 79.167 | 96 | 13 | 1 | 1 | 89 | 1 | 96 | 8.48e-43 | 134 |
| MsG0280010670.01.T01 | MTR_2g089970 | 64.045 | 89 | 32 | 0 | 1 | 89 | 1 | 89 | 7.71e-37 | 119 |
| MsG0280010670.01.T01 | MTR_2g089990 | 64.045 | 89 | 32 | 0 | 1 | 89 | 1 | 89 | 1.35e-35 | 116 |
| MsG0280010670.01.T01 | MTR_4g045990 | 73.626 | 91 | 20 | 3 | 1 | 89 | 1 | 89 | 1.01e-34 | 114 |
| MsG0280010670.01.T01 | MTR_2g089945 | 62.921 | 89 | 30 | 2 | 2 | 89 | 5 | 91 | 1.63e-33 | 111 |
| MsG0280010670.01.T01 | MTR_2g089930 | 59.551 | 89 | 33 | 2 | 2 | 89 | 5 | 91 | 4.28e-32 | 107 |
| MsG0280010670.01.T01 | MTR_2g089950 | 59.341 | 91 | 28 | 2 | 1 | 89 | 1 | 84 | 1.79e-29 | 100 |
| MsG0280010670.01.T01 | MTR_2g089925 | 57.143 | 91 | 30 | 2 | 1 | 89 | 1 | 84 | 3.89e-27 | 94.7 |
| MsG0280010670.01.T01 | MTR_2g481150 | 53.933 | 89 | 35 | 2 | 1 | 88 | 1 | 84 | 3.66e-26 | 92.4 |
| MsG0280010670.01.T01 | MTR_2g481160 | 55.556 | 90 | 32 | 3 | 1 | 88 | 1 | 84 | 6.61e-26 | 92.0 |
| MsG0280010670.01.T01 | MTR_2g089865 | 57.647 | 85 | 33 | 2 | 2 | 85 | 5 | 87 | 1.81e-23 | 90.9 |
| MsG0280010670.01.T01 | MTR_2g081155 | 50.549 | 91 | 33 | 5 | 1 | 88 | 1 | 82 | 4.42e-22 | 82.0 |
| MsG0280010670.01.T01 | MTR_2g089835 | 46.667 | 90 | 45 | 2 | 1 | 89 | 1 | 88 | 5.48e-20 | 77.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010670.01.T01 | AT4G10270 | 64.444 | 90 | 31 | 1 | 1 | 89 | 1 | 90 | 1.41e-32 | 108 |
| MsG0280010670.01.T01 | AT4G10265 | 57.303 | 89 | 30 | 2 | 1 | 88 | 1 | 82 | 3.38e-30 | 102 |
Find 15 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCAATCAGAGGAATCTTTA+AGG | 0.315897 | 2:+73147104 | MsG0280010670.01.T01:CDS |
| AGTTATGTACTTGAGTTGTT+GGG | 0.380679 | 2:+73147129 | MsG0280010670.01.T01:CDS |
| AAGTTATGTACTTGAGTTGT+TGG | 0.395020 | 2:+73147128 | MsG0280010670.01.T01:CDS |
| CAACCAAAGCTCCTCTTAGT+TGG | 0.412441 | 2:-73147155 | None:intergenic |
| TTCTAATTCTAGACAACAAA+AGG | 0.422761 | 2:+73147078 | MsG0280010670.01.T01:CDS |
| AACCAAAGCTCCTCTTAGTT+GGG | 0.489304 | 2:-73147154 | None:intergenic |
| AGCAGAGCATGGGTAGCTGC+AGG | 0.497606 | 2:+73146905 | MsG0280010670.01.T01:CDS |
| GTGGAAGCATTGAAAGATCA+AGG | 0.506795 | 2:+73146941 | MsG0280010670.01.T01:CDS |
| TTTGAACTGACTTTAGGGTA+TGG | 0.511934 | 2:-73146979 | None:intergenic |
| GTTATGTACTTGAGTTGTTG+GGG | 0.552188 | 2:+73147130 | MsG0280010670.01.T01:CDS |
| GAGTTCAACAAGCAGAGCAT+GGG | 0.554434 | 2:+73146895 | MsG0280010670.01.T01:CDS |
| TGCAGGCAGTGTTGCAGTTG+TGG | 0.558647 | 2:+73146922 | MsG0280010670.01.T01:CDS |
| TGAGTTCAACAAGCAGAGCA+TGG | 0.560896 | 2:+73146894 | MsG0280010670.01.T01:CDS |
| AAGATCAAGGAATATGCAGA+TGG | 0.613727 | 2:+73146954 | MsG0280010670.01.T01:CDS |
| ACAAAAGGCAAAGCAATCAG+AGG | 0.651467 | 2:+73147093 | MsG0280010670.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTCTAATTCTAGACAACAAA+AGG | + | Chr2:73147078-73147097 | MsG0280010670.01.T01:CDS | 25.0% |
| ! | AAGTTATGTACTTGAGTTGT+TGG | + | Chr2:73147128-73147147 | MsG0280010670.01.T01:CDS | 30.0% |
| ! | AGTTATGTACTTGAGTTGTT+GGG | + | Chr2:73147129-73147148 | MsG0280010670.01.T01:CDS | 30.0% |
| !! | GTGATTTTGAACTGACTTTA+GGG | - | Chr2:73146987-73147006 | None:intergenic | 30.0% |
| AAGATCAAGGAATATGCAGA+TGG | + | Chr2:73146954-73146973 | MsG0280010670.01.T01:CDS | 35.0% | |
| AGCAATCAGAGGAATCTTTA+AGG | + | Chr2:73147104-73147123 | MsG0280010670.01.T01:CDS | 35.0% | |
| TTCTTCTTCTTCTTCTGCTA+TGG | + | Chr2:73147054-73147073 | MsG0280010670.01.T01:CDS | 35.0% | |
| ! | GTTATGTACTTGAGTTGTTG+GGG | + | Chr2:73147130-73147149 | MsG0280010670.01.T01:CDS | 35.0% |
| !! | CGTGATTTTGAACTGACTTT+AGG | - | Chr2:73146988-73147007 | None:intergenic | 35.0% |
| !! | TTTGAACTGACTTTAGGGTA+TGG | - | Chr2:73146982-73147001 | None:intergenic | 35.0% |
| ACAAAAGGCAAAGCAATCAG+AGG | + | Chr2:73147093-73147112 | MsG0280010670.01.T01:CDS | 40.0% | |
| !! | GTGGAAGCATTGAAAGATCA+AGG | + | Chr2:73146941-73146960 | MsG0280010670.01.T01:CDS | 40.0% |
| GAGTTCAACAAGCAGAGCAT+GGG | + | Chr2:73146895-73146914 | MsG0280010670.01.T01:CDS | 45.0% | |
| TGAGTTCAACAAGCAGAGCA+TGG | + | Chr2:73146894-73146913 | MsG0280010670.01.T01:CDS | 45.0% | |
| TGCAGGCAGTGTTGCAGTTG+TGG | + | Chr2:73146922-73146941 | MsG0280010670.01.T01:CDS | 55.0% | |
| AGCAGAGCATGGGTAGCTGC+AGG | + | Chr2:73146905-73146924 | MsG0280010670.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 73146893 | 73147162 | 73146893 | ID=MsG0280010670.01;Name=MsG0280010670.01 |
| Chr2 | mRNA | 73146893 | 73147162 | 73146893 | ID=MsG0280010670.01.T01;Parent=MsG0280010670.01;Name=MsG0280010670.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|89 |
| Chr2 | exon | 73146893 | 73147162 | 73146893 | ID=MsG0280010670.01.T01:exon:19083;Parent=MsG0280010670.01.T01 |
| Chr2 | CDS | 73146893 | 73147162 | 73146893 | ID=MsG0280010670.01.T01:cds;Parent=MsG0280010670.01.T01 |
| Gene Sequence |
| Protein sequence |
