Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010673.01.T01 | XP_013465011.1 | 84.783 | 92 | 14 | 0 | 1 | 92 | 1 | 92 | 6.74E-44 | 147 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010673.01.T01 | A0A072VBQ8 | 84.783 | 92 | 14 | 0 | 1 | 92 | 1 | 92 | 3.22e-44 | 147 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003430.01 | MsG0280010673.01 | 0.827812 | 1.272423e-54 | 1.241729e-51 |
| MsG0280010663.01 | MsG0280010673.01 | 0.801506 | 8.719820e-49 | 4.171453e-46 |
| MsG0280010666.01 | MsG0280010673.01 | 0.848070 | 7.746016e-60 | 1.404487e-56 |
| MsG0280010669.01 | MsG0280010673.01 | 0.840582 | 7.970900e-58 | 1.140452e-54 |
| MsG0280010670.01 | MsG0280010673.01 | 0.815105 | 1.096293e-51 | 7.489246e-49 |
| MsG0280010671.01 | MsG0280010673.01 | 0.822772 | 1.980985e-53 | 1.673999e-50 |
| MsG0280010673.01 | MsG0780036060.01 | 0.805430 | 1.339888e-49 | 7.087295e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010673.01.T01 | MTR_2g089905 | 84.783 | 92 | 14 | 0 | 1 | 92 | 1 | 92 | 8.17e-48 | 147 |
| MsG0280010673.01.T01 | MTR_2g089940 | 85.870 | 92 | 12 | 1 | 1 | 92 | 1 | 91 | 8.04e-46 | 142 |
| MsG0280010673.01.T01 | MTR_2g089985 | 85.870 | 92 | 12 | 1 | 1 | 92 | 1 | 91 | 8.04e-46 | 142 |
| MsG0280010673.01.T01 | MTR_2g089960 | 85.870 | 92 | 12 | 1 | 1 | 92 | 1 | 91 | 8.04e-46 | 142 |
| MsG0280010673.01.T01 | MTR_2g089965 | 89.583 | 96 | 6 | 1 | 1 | 92 | 1 | 96 | 1.75e-45 | 141 |
| MsG0280010673.01.T01 | MTR_2g089970 | 63.043 | 92 | 31 | 2 | 1 | 92 | 1 | 89 | 1.21e-33 | 111 |
| MsG0280010673.01.T01 | MTR_2g089990 | 65.217 | 92 | 29 | 2 | 1 | 92 | 1 | 89 | 1.59e-33 | 111 |
| MsG0280010673.01.T01 | MTR_4g045990 | 71.277 | 94 | 20 | 3 | 1 | 92 | 1 | 89 | 5.80e-33 | 109 |
| MsG0280010673.01.T01 | MTR_2g089945 | 59.783 | 92 | 31 | 2 | 2 | 92 | 5 | 91 | 5.27e-32 | 107 |
| MsG0280010673.01.T01 | MTR_2g089930 | 55.435 | 92 | 35 | 2 | 2 | 92 | 5 | 91 | 5.20e-31 | 105 |
| MsG0280010673.01.T01 | MTR_2g089950 | 55.319 | 94 | 30 | 2 | 1 | 92 | 1 | 84 | 6.57e-28 | 97.1 |
| MsG0280010673.01.T01 | MTR_2g089925 | 55.319 | 94 | 30 | 3 | 1 | 92 | 1 | 84 | 3.62e-26 | 92.4 |
| MsG0280010673.01.T01 | MTR_2g481150 | 51.087 | 92 | 36 | 2 | 1 | 91 | 1 | 84 | 1.22e-24 | 89.0 |
| MsG0280010673.01.T01 | MTR_2g481160 | 51.087 | 92 | 36 | 2 | 1 | 91 | 1 | 84 | 4.07e-24 | 87.4 |
| MsG0280010673.01.T01 | MTR_2g081155 | 48.936 | 94 | 33 | 5 | 1 | 91 | 1 | 82 | 3.90e-22 | 82.4 |
| MsG0280010673.01.T01 | MTR_2g089865 | 54.545 | 88 | 34 | 2 | 2 | 88 | 5 | 87 | 7.16e-22 | 87.0 |
| MsG0280010673.01.T01 | MTR_2g089835 | 48.387 | 93 | 42 | 3 | 1 | 92 | 1 | 88 | 3.04e-21 | 80.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010673.01.T01 | AT4G10270 | 60.215 | 93 | 33 | 2 | 1 | 92 | 1 | 90 | 7.16e-29 | 99.8 |
| MsG0280010673.01.T01 | AT4G10265 | 55.435 | 92 | 30 | 3 | 1 | 91 | 1 | 82 | 2.82e-28 | 97.8 |
Find 15 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATTGATCAAAGATATTAAT+TGG | 0.110664 | 2:-73183214 | None:intergenic |
| TTCCTCTTCTTCTTCTGCTT+TGG | 0.275504 | 2:+73183107 | MsG0280010673.01.T01:CDS |
| AGTCATGTACTTGAGTTGTT+GGG | 0.407346 | 2:+73183188 | MsG0280010673.01.T01:CDS |
| AAGTCATGTACTTGAGTTGT+TGG | 0.447760 | 2:+73183187 | MsG0280010673.01.T01:CDS |
| AAGATCAAGGAATATGTAGA+TGG | 0.463290 | 2:+73183004 | MsG0280010673.01.T01:CDS |
| ATTGATCAAAGATATTAATT+GGG | 0.468411 | 2:-73183213 | None:intergenic |
| AACCAAAGCAGAAGAAGAAG+AGG | 0.481950 | 2:-73183109 | None:intergenic |
| AGCAGAAGAAGAAGAGGAAG+AGG | 0.487847 | 2:-73183103 | None:intergenic |
| GTAGAAGCATTGAAAGATCA+AGG | 0.504268 | 2:+73182991 | MsG0280010673.01.T01:CDS |
| AGCAGAGCATGGGTAGCTGC+AGG | 0.522642 | 2:+73182955 | MsG0280010673.01.T01:CDS |
| TGAGTTCAACAAGCAGAGCA+TGG | 0.554075 | 2:+73182944 | MsG0280010673.01.T01:CDS |
| GTCATGTACTTGAGTTGTTG+GGG | 0.566556 | 2:+73183189 | MsG0280010673.01.T01:CDS |
| AGCAATCAGAGGAATCTTTG+AGG | 0.586212 | 2:+73183163 | MsG0280010673.01.T01:CDS |
| GAGTTCAACAAGCAGAGCAT+GGG | 0.590239 | 2:+73182945 | MsG0280010673.01.T01:CDS |
| AGAGAATGCAAAGCAATCAG+AGG | 0.632612 | 2:+73183152 | MsG0280010673.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGATCAAGGAATATGTAGA+TGG | + | Chr2:73183004-73183023 | MsG0280010673.01.T01:CDS | 30.0% | |
| ! | AAGTCATGTACTTGAGTTGT+TGG | + | Chr2:73183187-73183206 | MsG0280010673.01.T01:CDS | 35.0% |
| ! | AGTCATGTACTTGAGTTGTT+GGG | + | Chr2:73183188-73183207 | MsG0280010673.01.T01:CDS | 35.0% |
| !! | GTAGAAGCATTGAAAGATCA+AGG | + | Chr2:73182991-73183010 | MsG0280010673.01.T01:CDS | 35.0% |
| AACCAAAGCAGAAGAAGAAG+AGG | - | Chr2:73183112-73183131 | None:intergenic | 40.0% | |
| AGAGAATGCAAAGCAATCAG+AGG | + | Chr2:73183152-73183171 | MsG0280010673.01.T01:CDS | 40.0% | |
| AGCAATCAGAGGAATCTTTG+AGG | + | Chr2:73183163-73183182 | MsG0280010673.01.T01:CDS | 40.0% | |
| TTCCTCTTCTTCTTCTGCTT+TGG | + | Chr2:73183107-73183126 | MsG0280010673.01.T01:CDS | 40.0% | |
| ! | GTCATGTACTTGAGTTGTTG+GGG | + | Chr2:73183189-73183208 | MsG0280010673.01.T01:CDS | 40.0% |
| AGCAGAAGAAGAAGAGGAAG+AGG | - | Chr2:73183106-73183125 | None:intergenic | 45.0% | |
| GAGTTCAACAAGCAGAGCAT+GGG | + | Chr2:73182945-73182964 | MsG0280010673.01.T01:CDS | 45.0% | |
| TGAGTTCAACAAGCAGAGCA+TGG | + | Chr2:73182944-73182963 | MsG0280010673.01.T01:CDS | 45.0% | |
| AGCAGAGCATGGGTAGCTGC+AGG | + | Chr2:73182955-73182974 | MsG0280010673.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 73182943 | 73183221 | 73182943 | ID=MsG0280010673.01;Name=MsG0280010673.01 |
| Chr2 | mRNA | 73182943 | 73183221 | 73182943 | ID=MsG0280010673.01.T01;Parent=MsG0280010673.01;Name=MsG0280010673.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|92 |
| Chr2 | exon | 73182943 | 73183221 | 73182943 | ID=MsG0280010673.01.T01:exon:19631;Parent=MsG0280010673.01.T01 |
| Chr2 | CDS | 73182943 | 73183221 | 73182943 | ID=MsG0280010673.01.T01:cds;Parent=MsG0280010673.01.T01 |
| Gene Sequence |
| Protein sequence |
