AlfalfaGEDB | MsG0280010669.01

M. sativa cultivar ZhongmuNo.1 / MsG0280010669.01

KEGG: -
GO: -
PFAM: PF12609

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010669.01.T01	XP_024633071.1	95.604	91	4	0	1	91	1	91	1.79E-57	181

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010669.01.T01	I3SV61	95.604	91	4	0	1	91	1	91	8.56e-58	181

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180003430.01	MsG0280010669.01	0.847885	8.711936e-60	1.570668e-56
MsG0180005028.01	MsG0280010669.01	0.837185	6.034361e-57	7.777464e-54
MsG0280010663.01	MsG0280010669.01	0.893371	6.692601e-75	6.357330e-71
MsG0280010666.01	MsG0280010669.01	0.931479	3.554135e-94	2.245955e-89
MsG0280010669.01	MsG0280010670.01	0.813802	2.128957e-51	1.404085e-48
MsG0280010669.01	MsG0280010671.01	0.894900	1.594912e-75	1.617100e-71
MsG0280010669.01	MsG0280010673.01	0.840582	7.970900e-58	1.140452e-54
MsG0280010669.01	MsG0380015543.01	0.814617	1.406473e-51	9.483082e-49
MsG0280010669.01	MsG0680030288.01	0.808895	2.473091e-50	1.431049e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010669.01.T01	MTR_2g089930	93.407	91	6	0	1	91	1	91	6.58e-61	180
MsG0280010669.01.T01	MTR_2g089945	94.505	91	5	0	1	91	1	91	7.67e-61	180
MsG0280010669.01.T01	MTR_2g089865	91.954	87	7	0	1	87	1	87	5.51e-50	159
MsG0280010669.01.T01	MTR_4g045990	74.118	85	19	2	9	91	6	89	1.60e-37	121
MsG0280010669.01.T01	MTR_2g089970	63.333	90	28	3	5	91	2	89	3.40e-34	113
MsG0280010669.01.T01	MTR_2g089950	67.901	81	20	3	11	91	10	84	3.60e-32	107
MsG0280010669.01.T01	MTR_2g089990	64.286	84	25	3	11	91	8	89	8.99e-32	107
MsG0280010669.01.T01	MTR_2g089905	61.957	92	29	3	5	91	2	92	1.35e-30	103
MsG0280010669.01.T01	MTR_2g089925	64.198	81	23	3	11	91	10	84	1.49e-30	103
MsG0280010669.01.T01	MTR_2g089940	62.637	91	29	2	5	91	2	91	7.40e-30	102
MsG0280010669.01.T01	MTR_2g089985	62.637	91	29	2	5	91	2	91	7.40e-30	102
MsG0280010669.01.T01	MTR_2g089960	62.637	91	29	2	5	91	2	91	7.40e-30	102
MsG0280010669.01.T01	MTR_2g089965	56.250	96	32	2	5	91	2	96	4.20e-28	97.8
MsG0280010669.01.T01	MTR_2g481160	55.814	86	35	1	5	90	2	84	1.98e-26	93.2
MsG0280010669.01.T01	MTR_2g081155	55.172	87	32	3	5	90	2	82	4.04e-26	92.4
MsG0280010669.01.T01	MTR_2g481150	53.488	86	37	1	5	90	2	84	1.08e-25	91.7
MsG0280010669.01.T01	MTR_2g089835	52.874	87	39	2	6	91	3	88	1.83e-23	85.9
MsG0280010669.01.T01	MTR_2g011690	46.053	76	35	3	19	91	17	89	2.43e-11	55.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010669.01.T01	AT4G10265	65.517	87	25	1	5	91	2	83	5.99e-37	119
MsG0280010669.01.T01	AT4G10270	67.416	89	27	1	5	91	2	90	5.64e-36	117

Find 22 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATTTGTTGCTACCAAATTTA+AGG	0.083204	2:+73143230	MsG0280010669.01.T01:CDS
CTTCTTGTCATCCTTAAATT+TGG	0.215497	2:-73143241	None:intergenic
CAAAGACTCCTCTGCCTGTT+TGG	0.289223	2:-73143265	None:intergenic
CTGTCATGTACTTGAGCTGC+TGG	0.406277	2:+73143292	MsG0280010669.01.T01:CDS
CTATGTACAAATGAATCAAT+TGG	0.410350	2:-73143319	None:intergenic
GGATGACAAGAAGGCCAAAC+AGG	0.439731	2:+73143251	MsG0280010669.01.T01:CDS
AACAGGCAGAGGAGTCTTTG+AGG	0.455186	2:+73143268	MsG0280010669.01.T01:CDS
TGTCATGTACTTGAGCTGCT+GGG	0.470465	2:+73143293	MsG0280010669.01.T01:CDS
GGCTGCAAGTGTTGGAGTTG+TGG	0.471925	2:+73143089	MsG0280010669.01.T01:CDS
TATGTACAAATGAATCAATT+GGG	0.493533	2:-73143318	None:intergenic
CAAGAAGGCCAAACAGGCAG+AGG	0.502891	2:+73143257	MsG0280010669.01.T01:CDS
CAAATTTAAGGATGACAAGA+AGG	0.525172	2:+73143242	MsG0280010669.01.T01:CDS
AGCAACAAGCAAAGCATGGA+TGG	0.538950	2:+73143065	MsG0280010669.01.T01:CDS
CTTGAGAAATTGATCTAACA+CGG	0.546721	2:-73143185	None:intergenic
GTCATGTACTTGAGCTGCTG+GGG	0.565171	2:+73143294	MsG0280010669.01.T01:CDS
CAACAGCAACAAGCAAAGCA+TGG	0.569709	2:+73143061	MsG0280010669.01.T01:CDS
TGGATGGTGGCTGCAAGTGT+TGG	0.573709	2:+73143081	MsG0280010669.01.T01:CDS
TGCAAGTGTTGGAGTTGTGG+AGG	0.602761	2:+73143092	MsG0280010669.01.T01:CDS
AAGATCAAGCTGGTATCTGT+AGG	0.603543	2:+73143121	MsG0280010669.01.T01:CDS
ATCAAGCTGGTATCTGTAGG+TGG	0.610789	2:+73143124	MsG0280010669.01.T01:CDS
AACAAGCAAAGCATGGATGG+TGG	0.650577	2:+73143068	MsG0280010669.01.T01:CDS
GAGGCATTGAAAGATCAAGC+TGG	0.657595	2:+73143111	MsG0280010669.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTTGTTGCTACCAAATTTA+AGG	+	Chr2:73143230-73143249	MsG0280010669.01.T01:CDS	25.0%
	CAAATTTAAGGATGACAAGA+AGG	+	Chr2:73143242-73143261	MsG0280010669.01.T01:CDS	30.0%
	CTTCTTGTCATCCTTAAATT+TGG	-	Chr2:73143244-73143263	None:intergenic	30.0%
	CTTGAGAAATTGATCTAACA+CGG	-	Chr2:73143188-73143207	None:intergenic	30.0%
!	TTGATCTAACACGGTTTTTA+AGG	-	Chr2:73143179-73143198	None:intergenic	30.0%
	AAGATCAAGCTGGTATCTGT+AGG	+	Chr2:73143121-73143140	MsG0280010669.01.T01:CDS	40.0%
	AACAAGCAAAGCATGGATGG+TGG	+	Chr2:73143068-73143087	MsG0280010669.01.T01:CDS	45.0%
	AGCAACAAGCAAAGCATGGA+TGG	+	Chr2:73143065-73143084	MsG0280010669.01.T01:CDS	45.0%
	ATCAAGCTGGTATCTGTAGG+TGG	+	Chr2:73143124-73143143	MsG0280010669.01.T01:CDS	45.0%
	CAACAGCAACAAGCAAAGCA+TGG	+	Chr2:73143061-73143080	MsG0280010669.01.T01:CDS	45.0%
	TGTCATGTACTTGAGCTGCT+GGG	+	Chr2:73143293-73143312	MsG0280010669.01.T01:CDS	45.0%
!!	GAGGCATTGAAAGATCAAGC+TGG	+	Chr2:73143111-73143130	MsG0280010669.01.T01:CDS	45.0%
	AACAGGCAGAGGAGTCTTTG+AGG	+	Chr2:73143268-73143287	MsG0280010669.01.T01:CDS	50.0%
	CAAAGACTCCTCTGCCTGTT+TGG	-	Chr2:73143268-73143287	None:intergenic	50.0%
	CTGTCATGTACTTGAGCTGC+TGG	+	Chr2:73143292-73143311	MsG0280010669.01.T01:CDS	50.0%
	GGATGACAAGAAGGCCAAAC+AGG	+	Chr2:73143251-73143270	MsG0280010669.01.T01:CDS	50.0%
	GTCATGTACTTGAGCTGCTG+GGG	+	Chr2:73143294-73143313	MsG0280010669.01.T01:CDS	50.0%
!!	TGCAAGTGTTGGAGTTGTGG+AGG	+	Chr2:73143092-73143111	MsG0280010669.01.T01:CDS	50.0%
	CAAGAAGGCCAAACAGGCAG+AGG	+	Chr2:73143257-73143276	MsG0280010669.01.T01:CDS	55.0%
!	TGGATGGTGGCTGCAAGTGT+TGG	+	Chr2:73143081-73143100	MsG0280010669.01.T01:CDS	55.0%
!!	GGCTGCAAGTGTTGGAGTTG+TGG	+	Chr2:73143089-73143108	MsG0280010669.01.T01:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	73143051	73143326	73143051	ID=MsG0280010669.01;Name=MsG0280010669.01
Chr2	mRNA	73143051	73143326	73143051	ID=MsG0280010669.01.T01;Parent=MsG0280010669.01;Name=MsG0280010669.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|91
Chr2	exon	73143051	73143326	73143051	ID=MsG0280010669.01.T01:exon:19623;Parent=MsG0280010669.01.T01
Chr2	CDS	73143051	73143326	73143051	ID=MsG0280010669.01.T01:cds;Parent=MsG0280010669.01.T01

Gene Sequence	>MsG0280010669.01.T01 ATGAATCGTGCAACAGCAACAAGCAAAGCATGGATGGTGGCTGCAAGTGTTGGAGTTGTGGAGGCATTGAAAGATCAAGCTGGTATCTGTAGGTGGAACTATGCTTTAAGACAAGCTCAACAACACCTTAAAAACCGTGTTAGATCAATTTCTCAAGCAAAGAATTTCTCTTCTTCTTCATTTGTTGCTACCAAATTTAAGGATGACAAGAAGGCCAAACAGGCAGAGGAGTCTTTGAGGACTGTCATGTACTTGAGCTGCTGGGGTCCCAATTGA
Protein sequence	>MsG0280010669.01.T01 MNRATATSKAWMVAASVGVVEALKDQAGICRWNYALRQAQQHLKNRVRSISQAKNFSSSSFVATKFKDDKKAKQAEESLRTVMYLSCWGPN*