Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012070.01.T01 | KEH15779.1 | 94.257 | 296 | 17 | 0 | 1 | 296 | 1 | 296 | 0 | 587 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012070.01.T01 | Q9SZL8 | 27.306 | 271 | 170 | 6 | 31 | 296 | 68 | 316 | 1.64E-24 | 106 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012070.01.T01 | A0A072TE13 | 94.257 | 296 | 17 | 0 | 1 | 296 | 1 | 296 | 0.0 | 587 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380012070.01 | MsG0380012330.01 | 0.807584 | 4.707433e-50 | 2.631729e-47 |
| MsG0380012070.01 | MsG0380012361.01 | 0.800485 | 1.409603e-48 | 6.571349e-46 |
| MsG0380012070.01 | MsG0380014251.01 | 0.800510 | 1.393283e-48 | 6.499316e-46 |
| MsG0380012070.01 | MsG0580028692.01 | 0.814895 | 1.220501e-51 | 8.290572e-49 |
| MsG0380012070.01 | MsG0680032157.01 | 0.801659 | 8.113315e-49 | 3.896197e-46 |
| MsG0380012070.01 | MsG0780036080.01 | 0.808181 | 3.513214e-50 | 1.995341e-47 |
| MsG0380012070.01 | MsG0780036201.01 | 0.813152 | 2.958253e-51 | 1.916754e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012070.01.T01 | MTR_0558s0020 | 94.257 | 296 | 17 | 0 | 1 | 296 | 1 | 296 | 0.0 | 587 |
| MsG0380012070.01.T01 | MTR_1g083640 | 90.210 | 286 | 13 | 1 | 1 | 271 | 1 | 286 | 0.0 | 533 |
| MsG0380012070.01.T01 | MTR_8g446020 | 80.743 | 296 | 17 | 2 | 1 | 296 | 1 | 256 | 6.59e-171 | 473 |
| MsG0380012070.01.T01 | MTR_6g090505 | 93.478 | 230 | 15 | 0 | 66 | 295 | 1 | 230 | 2.96e-161 | 453 |
| MsG0380012070.01.T01 | MTR_2g014100 | 88.163 | 245 | 21 | 1 | 1 | 237 | 1 | 245 | 2.76e-158 | 441 |
| MsG0380012070.01.T01 | MTR_0337s0040 | 75.479 | 261 | 25 | 1 | 1 | 261 | 1 | 222 | 2.61e-138 | 390 |
| MsG0380012070.01.T01 | MTR_3g008240 | 90.640 | 203 | 19 | 0 | 1 | 203 | 1 | 203 | 5.45e-135 | 380 |
| MsG0380012070.01.T01 | MTR_4g040430 | 91.133 | 203 | 13 | 2 | 1 | 203 | 1 | 198 | 5.01e-133 | 375 |
| MsG0380012070.01.T01 | MTR_5g071590 | 83.889 | 180 | 17 | 1 | 1 | 168 | 1 | 180 | 5.46e-109 | 314 |
| MsG0380012070.01.T01 | MTR_0019s0290 | 89.474 | 152 | 16 | 0 | 83 | 234 | 9 | 160 | 5.11e-99 | 288 |
| MsG0380012070.01.T01 | MTR_1g055240 | 94.406 | 143 | 8 | 0 | 91 | 233 | 2 | 144 | 9.78e-99 | 287 |
| MsG0380012070.01.T01 | MTR_1281s0010 | 91.743 | 109 | 9 | 0 | 20 | 128 | 1 | 109 | 2.51e-69 | 211 |
| MsG0380012070.01.T01 | MTR_4g008820 | 88.750 | 80 | 9 | 0 | 1 | 80 | 125 | 204 | 3.13e-39 | 145 |
| MsG0380012070.01.T01 | MTR_8g046060 | 82.955 | 88 | 7 | 2 | 1 | 80 | 91 | 178 | 9.57e-38 | 133 |
| MsG0380012070.01.T01 | MTR_6g051425 | 30.315 | 254 | 152 | 5 | 41 | 289 | 22 | 255 | 4.62e-30 | 116 |
| MsG0380012070.01.T01 | MTR_1g070355 | 27.491 | 291 | 184 | 7 | 7 | 292 | 43 | 311 | 1.07e-21 | 95.5 |
| MsG0380012070.01.T01 | MTR_0337s0050 | 83.333 | 36 | 6 | 0 | 260 | 295 | 14 | 49 | 2.62e-15 | 70.1 |
| MsG0380012070.01.T01 | MTR_0402s0040 | 25.946 | 185 | 118 | 4 | 50 | 234 | 40 | 205 | 3.33e-15 | 75.9 |
| MsG0380012070.01.T01 | MTR_1g013060 | 29.954 | 217 | 131 | 8 | 41 | 254 | 29 | 227 | 1.28e-14 | 72.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012070.01.T01 | AT4G38180 | 27.306 | 271 | 170 | 6 | 31 | 296 | 68 | 316 | 1.67e-25 | 106 |
| MsG0380012070.01.T01 | AT5G28530 | 23.846 | 260 | 168 | 6 | 41 | 288 | 60 | 301 | 8.70e-14 | 72.0 |
| MsG0380012070.01.T01 | AT5G28530 | 23.846 | 260 | 168 | 6 | 41 | 288 | 60 | 301 | 9.17e-14 | 72.0 |
| MsG0380012070.01.T01 | AT5G28530 | 23.846 | 260 | 168 | 6 | 41 | 288 | 60 | 301 | 9.17e-14 | 72.0 |
| MsG0380012070.01.T01 | AT1G10240 | 22.000 | 250 | 175 | 7 | 50 | 296 | 62 | 294 | 3.29e-11 | 64.3 |
| MsG0380012070.01.T01 | AT1G10240 | 22.000 | 250 | 175 | 7 | 50 | 296 | 62 | 294 | 3.29e-11 | 64.3 |
| MsG0380012070.01.T01 | AT4G15090 | 25.101 | 247 | 164 | 6 | 51 | 296 | 66 | 292 | 6.41e-11 | 63.5 |
| MsG0380012070.01.T01 | AT4G15090 | 25.101 | 247 | 164 | 6 | 51 | 296 | 66 | 292 | 6.41e-11 | 63.5 |
| MsG0380012070.01.T01 | AT4G15090 | 25.101 | 247 | 164 | 6 | 51 | 296 | 66 | 292 | 6.41e-11 | 63.5 |
| MsG0380012070.01.T01 | AT4G15090 | 25.101 | 247 | 164 | 6 | 51 | 296 | 66 | 292 | 6.41e-11 | 63.5 |
| MsG0380012070.01.T01 | AT4G15090 | 25.101 | 247 | 164 | 6 | 51 | 296 | 66 | 292 | 6.41e-11 | 63.5 |
Find 43 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGATGTTGGCCTGTTGTT+AGG | 0.302249 | 3:-10472752 | None:intergenic |
| TTTCAATCTATTAAAGAGAT+TGG | 0.308439 | 3:+10472575 | MsG0380012070.01.T01:CDS |
| GCCGATATTGATTGTTTGTT+TGG | 0.344105 | 3:-10473309 | None:intergenic |
| CCCATGATTCTTTATTACTA+AGG | 0.356218 | 3:-10472541 | None:intergenic |
| GAGAACGTCTTATGAAGTTA+TGG | 0.394356 | 3:+10473018 | MsG0380012070.01.T01:CDS |
| ACCAAACAAACAATCAATAT+CGG | 0.402779 | 3:+10473308 | MsG0380012070.01.T01:CDS |
| ATGTCTATAAGATATATAGA+TGG | 0.423693 | 3:+10472873 | MsG0380012070.01.T01:CDS |
| GAAGTTATGGGTCAAGGAGT+TGG | 0.428685 | 3:+10473031 | MsG0380012070.01.T01:CDS |
| AACAATCAATATCGGCCATT+AGG | 0.428800 | 3:+10473316 | MsG0380012070.01.T01:CDS |
| CGAGAAGTAAATGAAGTTCA+AGG | 0.430014 | 3:+10472956 | MsG0380012070.01.T01:CDS |
| GAAAGGGTTGGTTGTAAAGC+TGG | 0.447509 | 3:+10472821 | MsG0380012070.01.T01:CDS |
| TCTAATGACAATTCGTCAAA+AGG | 0.456711 | 3:+10473099 | MsG0380012070.01.T01:CDS |
| AGAACGTCTTATGAAGTTAT+GGG | 0.466325 | 3:+10473019 | MsG0380012070.01.T01:CDS |
| GTCTGCTTAATCCTAACAAC+AGG | 0.466827 | 3:+10472741 | MsG0380012070.01.T01:CDS |
| ACAAAATAAGAAATCGGATA+AGG | 0.469178 | 3:+10472670 | MsG0380012070.01.T01:CDS |
| GTTGTAATAAATTCTAAAGC+TGG | 0.473712 | 3:+10472986 | MsG0380012070.01.T01:CDS |
| CCCTTAGTAATAAAGAATCA+TGG | 0.477731 | 3:+10472540 | MsG0380012070.01.T01:CDS |
| AGTACAAAATACTAACCTAA+TGG | 0.480025 | 3:-10473331 | None:intergenic |
| TTACTTCTCGAAATGATCTC+AGG | 0.488412 | 3:-10472943 | None:intergenic |
| CCATGATTCTTTATTACTAA+GGG | 0.489319 | 3:-10472540 | None:intergenic |
| CAATTCACAAAATAAGAAAT+CGG | 0.490263 | 3:+10472664 | MsG0380012070.01.T01:CDS |
| CGTTATGTTTGTAACAAACA+AGG | 0.494754 | 3:+10472704 | MsG0380012070.01.T01:CDS |
| CTCAGGTAACACAAATGCTC+TGG | 0.496027 | 3:-10472926 | None:intergenic |
| TGTCTTCTGCAGCATCAACT+TGG | 0.502977 | 3:-10473204 | None:intergenic |
| TTACAACCAACCCTTTCCTC+AGG | 0.505082 | 3:-10472815 | None:intergenic |
| TAACAAACAAGGTTTCAAGA+AGG | 0.511682 | 3:+10472715 | MsG0380012070.01.T01:CDS |
| AAATGCTCTGGTTTATGTAA+TGG | 0.514358 | 3:-10472914 | None:intergenic |
| GGTAATTATGCATATAACAC+TGG | 0.523547 | 3:+10472608 | MsG0380012070.01.T01:CDS |
| TGTCTATAAGATATATAGAT+GGG | 0.523615 | 3:+10472874 | MsG0380012070.01.T01:CDS |
| TTTAAGTCCGAAAACCGAAA+AGG | 0.524077 | 3:-10473070 | None:intergenic |
| TTCACAAACTCAATAACCAC+TGG | 0.531770 | 3:-10472779 | None:intergenic |
| AACAAACAAGGTTTCAAGAA+GGG | 0.554561 | 3:+10472716 | MsG0380012070.01.T01:CDS |
| CCTTAGTAATAAAGAATCAT+GGG | 0.555489 | 3:+10472541 | MsG0380012070.01.T01:CDS |
| CGTCAAAAGGAGATGGTGGT+TGG | 0.556443 | 3:+10473112 | MsG0380012070.01.T01:CDS |
| GACAATTCGTCAAAAGGAGA+TGG | 0.566160 | 3:+10473105 | MsG0380012070.01.T01:CDS |
| TAAGATATATAGATGGGATG+TGG | 0.567659 | 3:+10472880 | MsG0380012070.01.T01:CDS |
| GTTATGGGTCAAGGAGTTGG+AGG | 0.578191 | 3:+10473034 | MsG0380012070.01.T01:CDS |
| TCTTATGAAGTTATGGGTCA+AGG | 0.580120 | 3:+10473025 | MsG0380012070.01.T01:CDS |
| ACCACTGGACTGTCTGATGT+TGG | 0.589854 | 3:-10472764 | None:intergenic |
| ATATCAACTTCATCTGAGAG+TGG | 0.614980 | 3:+10472461 | MsG0380012070.01.T01:CDS |
| AATTCGTCAAAAGGAGATGG+TGG | 0.636402 | 3:+10473108 | MsG0380012070.01.T01:CDS |
| GGGTCAAGGAGTTGGAGGCA+CGG | 0.637387 | 3:+10473039 | MsG0380012070.01.T01:CDS |
| GCCAACATCAGACAGTCCAG+TGG | 0.711751 | 3:+10472763 | MsG0380012070.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGTCTATAAGATATATAGA+TGG | + | Chr3:10472873-10472892 | MsG0380012070.01.T01:CDS | 20.0% |
| !! | CAATTCACAAAATAAGAAAT+CGG | + | Chr3:10472664-10472683 | MsG0380012070.01.T01:CDS | 20.0% |
| !! | TGTCTATAAGATATATAGAT+GGG | + | Chr3:10472874-10472893 | MsG0380012070.01.T01:CDS | 20.0% |
| !! | TTTCAATCTATTAAAGAGAT+TGG | + | Chr3:10472575-10472594 | MsG0380012070.01.T01:CDS | 20.0% |
| ! | ACAAAATAAGAAATCGGATA+AGG | + | Chr3:10472670-10472689 | MsG0380012070.01.T01:CDS | 25.0% |
| ! | ACCAAACAAACAATCAATAT+CGG | + | Chr3:10473308-10473327 | MsG0380012070.01.T01:CDS | 25.0% |
| ! | CCATGATTCTTTATTACTAA+GGG | - | Chr3:10472543-10472562 | None:intergenic | 25.0% |
| ! | CCTTAGTAATAAAGAATCAT+GGG | + | Chr3:10472541-10472560 | MsG0380012070.01.T01:CDS | 25.0% |
| ! | TGGATATCATAGTAAAAAGA+TGG | - | Chr3:10473187-10473206 | None:intergenic | 25.0% |
| !! | AAAGAGATTGGAGATTTTTA+TGG | + | Chr3:10472587-10472606 | MsG0380012070.01.T01:CDS | 25.0% |
| !! | ATAACACTGGATTTTCTATT+AGG | + | Chr3:10472621-10472640 | MsG0380012070.01.T01:CDS | 25.0% |
| !!! | GTTGTAATAAATTCTAAAGC+TGG | + | Chr3:10472986-10473005 | MsG0380012070.01.T01:CDS | 25.0% |
| AACAAACAAGGTTTCAAGAA+GGG | + | Chr3:10472716-10472735 | MsG0380012070.01.T01:CDS | 30.0% | |
| AGAACGTCTTATGAAGTTAT+GGG | + | Chr3:10473019-10473038 | MsG0380012070.01.T01:CDS | 30.0% | |
| CCCATGATTCTTTATTACTA+AGG | - | Chr3:10472544-10472563 | None:intergenic | 30.0% | |
| CCCTTAGTAATAAAGAATCA+TGG | + | Chr3:10472540-10472559 | MsG0380012070.01.T01:CDS | 30.0% | |
| CGTTATGTTTGTAACAAACA+AGG | + | Chr3:10472704-10472723 | MsG0380012070.01.T01:CDS | 30.0% | |
| GGTAATTATGCATATAACAC+TGG | + | Chr3:10472608-10472627 | MsG0380012070.01.T01:CDS | 30.0% | |
| TAACAAACAAGGTTTCAAGA+AGG | + | Chr3:10472715-10472734 | MsG0380012070.01.T01:CDS | 30.0% | |
| TAAGATATATAGATGGGATG+TGG | + | Chr3:10472880-10472899 | MsG0380012070.01.T01:CDS | 30.0% | |
| TCTAATGACAATTCGTCAAA+AGG | + | Chr3:10473099-10473118 | MsG0380012070.01.T01:CDS | 30.0% | |
| ! | AAATGCTCTGGTTTATGTAA+TGG | - | Chr3:10472917-10472936 | None:intergenic | 30.0% |
| AACAATCAATATCGGCCATT+AGG | + | Chr3:10473316-10473335 | MsG0380012070.01.T01:CDS | 35.0% | |
| ATATCAACTTCATCTGAGAG+TGG | + | Chr3:10472461-10472480 | MsG0380012070.01.T01:CDS | 35.0% | |
| CGAGAAGTAAATGAAGTTCA+AGG | + | Chr3:10472956-10472975 | MsG0380012070.01.T01:CDS | 35.0% | |
| GAAAGAAAAACCTGAGGAAA+GGG | + | Chr3:10472805-10472824 | MsG0380012070.01.T01:CDS | 35.0% | |
| GAAAGTGAAAGAAAAACCTG+AGG | + | Chr3:10472799-10472818 | MsG0380012070.01.T01:CDS | 35.0% | |
| GAGAACGTCTTATGAAGTTA+TGG | + | Chr3:10473018-10473037 | MsG0380012070.01.T01:CDS | 35.0% | |
| TCTTATGAAGTTATGGGTCA+AGG | + | Chr3:10473025-10473044 | MsG0380012070.01.T01:CDS | 35.0% | |
| TGAAAGAAAAACCTGAGGAA+AGG | + | Chr3:10472804-10472823 | MsG0380012070.01.T01:CDS | 35.0% | |
| TTACTTCTCGAAATGATCTC+AGG | - | Chr3:10472946-10472965 | None:intergenic | 35.0% | |
| TTCACAAACTCAATAACCAC+TGG | - | Chr3:10472782-10472801 | None:intergenic | 35.0% | |
| TTTAAGTCCGAAAACCGAAA+AGG | - | Chr3:10473073-10473092 | None:intergenic | 35.0% | |
| ! | AAGACATAGCTAGCATTTTC+TGG | + | Chr3:10473221-10473240 | MsG0380012070.01.T01:CDS | 35.0% |
| ! | AGACATAGCTAGCATTTTCT+GGG | + | Chr3:10473222-10473241 | MsG0380012070.01.T01:CDS | 35.0% |
| ! | GAATCTTCCTTTTCGGTTTT+CGG | + | Chr3:10473063-10473082 | MsG0380012070.01.T01:CDS | 35.0% |
| ! | GCCGATATTGATTGTTTGTT+TGG | - | Chr3:10473312-10473331 | None:intergenic | 35.0% |
| AATTCGTCAAAAGGAGATGG+TGG | + | Chr3:10473108-10473127 | MsG0380012070.01.T01:CDS | 40.0% | |
| GACAATTCGTCAAAAGGAGA+TGG | + | Chr3:10473105-10473124 | MsG0380012070.01.T01:CDS | 40.0% | |
| GTCTGCTTAATCCTAACAAC+AGG | + | Chr3:10472741-10472760 | MsG0380012070.01.T01:CDS | 40.0% | |
| CTCAGGTAACACAAATGCTC+TGG | - | Chr3:10472929-10472948 | None:intergenic | 45.0% | |
| GAAAAACCTGAGGAAAGGGT+TGG | + | Chr3:10472809-10472828 | MsG0380012070.01.T01:CDS | 45.0% | |
| GAAGTTATGGGTCAAGGAGT+TGG | + | Chr3:10473031-10473050 | MsG0380012070.01.T01:CDS | 45.0% | |
| TGTCTTCTGCAGCATCAACT+TGG | - | Chr3:10473207-10473226 | None:intergenic | 45.0% | |
| TTACAACCAACCCTTTCCTC+AGG | - | Chr3:10472818-10472837 | None:intergenic | 45.0% | |
| ! | GCACGGAGAATCTTCCTTTT+CGG | + | Chr3:10473056-10473075 | MsG0380012070.01.T01:CDS | 45.0% |
| ! | TCTGATGTTGGCCTGTTGTT+AGG | - | Chr3:10472755-10472774 | None:intergenic | 45.0% |
| !! | GAAAGGGTTGGTTGTAAAGC+TGG | + | Chr3:10472821-10472840 | MsG0380012070.01.T01:CDS | 45.0% |
| ACCACTGGACTGTCTGATGT+TGG | - | Chr3:10472767-10472786 | None:intergenic | 50.0% | |
| GTTATGGGTCAAGGAGTTGG+AGG | + | Chr3:10473034-10473053 | MsG0380012070.01.T01:CDS | 50.0% | |
| ! | CGTCAAAAGGAGATGGTGGT+TGG | + | Chr3:10473112-10473131 | MsG0380012070.01.T01:CDS | 50.0% |
| ! | GCTAGCATTTTCTGGGCAGA+TGG | + | Chr3:10473229-10473248 | MsG0380012070.01.T01:CDS | 50.0% |
| GCCAACATCAGACAGTCCAG+TGG | + | Chr3:10472763-10472782 | MsG0380012070.01.T01:CDS | 55.0% | |
| GGGTCAAGGAGTTGGAGGCA+CGG | + | Chr3:10473039-10473058 | MsG0380012070.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 10472452 | 10473342 | 10472452 | ID=MsG0380012070.01;Name=MsG0380012070.01 |
| Chr3 | mRNA | 10472452 | 10473342 | 10472452 | ID=MsG0380012070.01.T01;Parent=MsG0380012070.01;Name=MsG0380012070.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|296 |
| Chr3 | exon | 10472452 | 10473342 | 10472452 | ID=MsG0380012070.01.T01:exon:21001;Parent=MsG0380012070.01.T01 |
| Chr3 | CDS | 10472452 | 10473342 | 10472452 | ID=MsG0380012070.01.T01:cds;Parent=MsG0380012070.01.T01 |
| Gene Sequence |
| Protein sequence |
