AlfalfaGEDB | MsG0580028692.01

M. sativa cultivar ZhongmuNo.1 / MsG0580028692.01

KEGG: -
GO: -
PFAM: PF13086 PF13087

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028692.01.T01	AES99078.2	81.818	913	118	6	25	918	1	884	0	1509

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028692.01.T01	Q00416	29.268	656	350	22	263	882	1279	1856	1.36E-56	217

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028692.01.T01	G7KFP1	81.818	913	118	6	25	918	1	884	0.0	1509

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000470.01	MsG0580028692.01	0.823518	1.326639e-53	1.144879e-50
MsG0180001182.01	MsG0580028692.01	0.800354	1.499261e-48	6.966221e-46
MsG0180001187.01	MsG0580028692.01	0.805941	1.046559e-49	5.609061e-47
MsG0180002234.01	MsG0580028692.01	0.813933	1.991314e-51	1.317943e-48
MsG0180002259.01	MsG0580028692.01	0.816680	4.880678e-52	3.481255e-49
MsG0180004275.01	MsG0580028692.01	0.808139	3.586445e-50	2.034611e-47
MsG0180006134.01	MsG0580028692.01	0.805161	1.525655e-49	8.014308e-47
MsG0380012070.01	MsG0580028692.01	0.814895	1.220501e-51	8.290572e-49
MsG0380013883.01	MsG0580028692.01	0.809581	1.763336e-50	1.039300e-47
MsG0380013896.01	MsG0580028692.01	0.804398	2.202107e-49	1.134209e-46
MsG0380014495.01	MsG0580028692.01	0.802391	5.735526e-49	2.806350e-46
MsG0480018653.01	MsG0580028692.01	0.805358	1.387237e-49	7.324312e-47
MsG0480021587.01	MsG0580028692.01	0.813704	2.236513e-51	1.471155e-48
MsG0380017855.01	MsG0580028692.01	0.815171	1.059895e-51	7.253543e-49
MsG0480018183.01	MsG0580028692.01	0.804468	2.129342e-49	1.098716e-46
MsG0580028169.01	MsG0580028692.01	0.820557	6.441566e-53	5.113641e-50
MsG0580028170.01	MsG0580028692.01	0.807234	5.584933e-50	3.094226e-47
MsG0580028692.01	MsG0580028810.01	0.821139	4.732542e-53	3.818401e-50
MsG0580028692.01	MsG0580028836.01	0.893190	7.916837e-75	7.468373e-71
MsG0580028692.01	MsG0680034150.01	0.800021	1.751843e-48	8.071159e-46
MsG0580028692.01	MsG0780036080.01	0.801284	9.681309e-49	4.605610e-46
MsG0580028692.01	MsG0780036201.01	0.837553	4.855817e-57	6.330020e-54
MsG0580028692.01	MsG0780038439.01	0.810022	1.417291e-50	8.451018e-48
MsG0580028692.01	MsG0880041863.01	0.819887	9.169603e-53	7.145361e-50
MsG0580028692.01	MsG0880044266.01	0.803855	2.856153e-49	1.450798e-46
MsG0280009421.01	MsG0580028692.01	0.829788	4.230755e-55	4.375235e-52

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028692.01.T01	MTR_5g076320	81.818	913	118	6	25	918	1	884	0.0	1509
MsG0580028692.01.T01	MTR_5g076310	69.307	909	219	13	34	933	2	859	0.0	1201
MsG0580028692.01.T01	MTR_5g076380	61.780	955	245	17	25	933	1	881	0.0	1072
MsG0580028692.01.T01	MTR_5g076500	61.784	908	234	18	46	914	12	845	0.0	1016
MsG0580028692.01.T01	MTR_5g076440	58.613	923	272	14	41	914	15	876	0.0	996
MsG0580028692.01.T01	MTR_5g076330	52.626	914	320	12	46	911	10	858	0.0	865
MsG0580028692.01.T01	MTR_3g064190	52.329	923	340	18	38	917	4	869	0.0	865
MsG0580028692.01.T01	MTR_5g076300	49.372	956	337	16	46	929	17	897	0.0	835
MsG0580028692.01.T01	MTR_3g436400	35.691	919	452	25	39	892	11	855	2.35e-163	520
MsG0580028692.01.T01	MTR_0189s0030	36.585	902	437	25	75	904	8	846	2.97e-154	503
MsG0580028692.01.T01	MTR_3g064210	45.740	446	209	5	485	905	499	936	9.17e-122	397
MsG0580028692.01.T01	MTR_3g064210	39.130	437	215	7	49	481	16	405	6.20e-80	281
MsG0580028692.01.T01	MTR_8g020390	38.854	314	171	9	575	879	457	758	2.92e-51	195
MsG0580028692.01.T01	MTR_8g020390	38.854	314	171	9	575	879	745	1046	9.72e-51	194
MsG0580028692.01.T01	MTR_8g020390	38.854	314	171	9	575	879	962	1263	1.31e-50	194
MsG0580028692.01.T01	MTR_5g053930	36.213	301	187	5	583	882	455	751	2.37e-45	176
MsG0580028692.01.T01	MTR_1g052700	35.926	270	168	5	617	884	41	307	3.44e-44	163
MsG0580028692.01.T01	MTR_2g105760	29.944	531	313	16	378	883	1577	2073	2.21e-43	172
MsG0580028692.01.T01	MTR_3g078040	37.453	267	103	7	67	330	56	261	9.25e-42	154
MsG0580028692.01.T01	MTR_5g013820	33.758	314	189	9	611	919	641	940	1.51e-38	156
MsG0580028692.01.T01	MTR_5g013820	33.758	314	189	9	611	919	641	940	1.88e-38	156
MsG0580028692.01.T01	MTR_7g092200	35.599	309	148	13	584	870	348	627	3.21e-29	125
MsG0580028692.01.T01	MTR_5g080800	51.402	107	47	1	799	905	12	113	1.70e-27	120
MsG0580028692.01.T01	MTR_1g040155	32.119	302	172	11	577	859	584	871	2.81e-26	116
MsG0580028692.01.T01	MTR_5g006890	33.096	281	153	10	611	870	282	548	2.77e-23	106
MsG0580028692.01.T01	MTR_5g006890	33.096	281	153	10	611	870	541	807	3.67e-23	106
MsG0580028692.01.T01	MTR_2g049990	33.096	281	153	10	611	870	541	807	3.83e-23	106
MsG0580028692.01.T01	MTR_2g049990	33.096	281	153	10	611	870	282	548	3.86e-23	105
MsG0580028692.01.T01	MTR_1g038815	25.698	537	311	22	352	853	892	1375	7.27e-20	96.3
MsG0580028692.01.T01	MTR_4g061370	43.243	111	52	2	67	177	527	626	1.06e-17	89.4
MsG0580028692.01.T01	MTR_8g063020	46.602	103	44	3	67	169	199	290	1.21e-17	85.9
MsG0580028692.01.T01	MTR_1g047040	62.963	54	15	1	843	891	1	54	2.28e-11	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028692.01.T01	AT1G65810	46.383	871	389	18	46	893	22	837	0.0	696
MsG0580028692.01.T01	AT1G65810	46.383	871	389	18	46	893	22	837	0.0	696
MsG0580028692.01.T01	AT1G65810	46.383	871	389	18	46	893	22	837	0.0	696
MsG0580028692.01.T01	AT1G65780	40.788	939	410	18	46	911	11	876	0.0	624
MsG0580028692.01.T01	AT5G37140	46.221	688	309	18	254	898	2	671	0.0	555
MsG0580028692.01.T01	AT5G52090	45.877	667	311	17	270	898	1	655	0.0	547
MsG0580028692.01.T01	AT5G37150	44.494	672	321	16	265	898	161	818	3.03e-178	538
MsG0580028692.01.T01	AT5G37150	40.816	147	72	5	42	188	5	136	2.57e-20	97.4
MsG0580028692.01.T01	AT4G05540	45.038	655	290	15	266	865	50	689	6.44e-171	514
MsG0580028692.01.T01	AT5G37165	49.510	408	193	9	499	897	1	404	6.05e-126	388
MsG0580028692.01.T01	AT5G37030	58.163	294	122	1	603	895	343	636	1.30e-115	368
MsG0580028692.01.T01	AT5G37030	50.000	156	73	3	324	477	198	350	5.07e-29	124
MsG0580028692.01.T01	AT5G37030	39.706	136	73	3	42	177	5	131	1.80e-18	90.9
MsG0580028692.01.T01	AT4G15570	28.622	566	318	18	328	877	256	751	4.70e-52	197
MsG0580028692.01.T01	AT4G15570	28.622	566	318	18	328	877	256	751	4.70e-52	197
MsG0580028692.01.T01	AT2G19120	37.419	310	181	8	575	879	742	1043	9.21e-50	191
MsG0580028692.01.T01	AT4G15570	36.066	305	183	6	578	877	365	662	3.56e-49	187
MsG0580028692.01.T01	AT4G15570	36.066	305	183	6	578	877	365	662	3.56e-49	187
MsG0580028692.01.T01	AT4G30100	35.873	315	187	9	575	883	905	1210	1.80e-46	181
MsG0580028692.01.T01	AT4G30100	35.873	315	187	9	575	883	905	1210	1.80e-46	181
MsG0580028692.01.T01	AT1G16800	28.352	522	329	16	378	879	1385	1881	5.10e-42	168
MsG0580028692.01.T01	AT1G16800	28.352	522	329	16	378	879	1427	1923	6.74e-42	167
MsG0580028692.01.T01	AT1G16800	28.352	522	329	16	378	879	1497	1993	7.01e-42	167
MsG0580028692.01.T01	AT5G37160	40.260	231	107	4	266	466	180	409	5.65e-40	154
MsG0580028692.01.T01	AT5G37160	36.552	145	74	6	52	191	24	155	6.85e-14	75.5
MsG0580028692.01.T01	AT5G47010	32.198	323	197	9	611	925	653	961	9.78e-38	154
MsG0580028692.01.T01	AT2G03270	34.333	300	170	13	582	870	339	622	5.23e-26	114
MsG0580028692.01.T01	AT5G35970	33.108	296	165	12	583	859	638	919	6.71e-25	112
MsG0580028692.01.T01	AT1G05460	31.385	325	169	12	611	911	542	836	1.09e-22	105
MsG0580028692.01.T01	AT1G05460	30.982	326	169	13	611	911	542	836	1.60e-22	104
MsG0580028692.01.T01	AT2G38770	32.946	258	146	11	611	853	1120	1365	5.77e-19	93.6
MsG0580028692.01.T01	AT1G08840	28.205	273	148	9	612	859	1050	1299	4.45e-16	84.0
MsG0580028692.01.T01	AT1G08840	28.205	273	148	9	612	859	1053	1302	4.57e-16	84.0
MsG0580028692.01.T01	AT1G08840	28.205	273	148	9	612	859	1037	1286	5.04e-16	84.0
MsG0580028692.01.T01	AT1G08840	28.205	273	148	9	612	859	1018	1267	5.23e-16	84.0

Find 159 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCAAGTCATTTAAGCTTTC+AGG	0.127572	5:+84854421	None:intergenic
AACATTCAAACATATTAATT+TGG	0.175529	5:+84851712	None:intergenic
TTTGAATACAAGAAGATATT+TGG	0.191015	5:-84853339	MsG0580028692.01.T01:CDS
ATGCTGATATCAAACTTATA+TGG	0.207233	5:-84853737	MsG0580028692.01.T01:CDS
TTAGTCTCCCATCCAGTATT+TGG	0.221963	5:+84853432	None:intergenic
TTAGGTGACATTGTGCTATT+TGG	0.241240	5:-84853546	MsG0580028692.01.T01:CDS
TGCACTGGCTTCACCTTTGC+TGG	0.242919	5:+84852979	None:intergenic
ATCCTCTTCACTTTCATTAC+TGG	0.258255	5:+84855404	None:intergenic
GTAAATGTTCGTTCTGTTGA+TGG	0.259767	5:-84852180	MsG0580028692.01.T01:CDS
TGCTCAAGTTTATGAAATTC+AGG	0.269725	5:-84852247	MsG0580028692.01.T01:CDS
TCATGTGTGAGGTTGATTTC+AGG	0.280798	5:+84854086	None:intergenic
ATTCTTTCAGTCGTTGAATC+TGG	0.291715	5:+84851818	None:intergenic
CTCATGTCAATGTTAAATTT+AGG	0.292464	5:+84853096	None:intergenic
TCTGATGAAGTTGAAAATAT+TGG	0.296974	5:-84854493	MsG0580028692.01.T01:CDS
ACGGCGTTTAGGAGGGAAAA+TGG	0.297803	5:-84851776	MsG0580028692.01.T01:CDS
AGATACTGTCTTTGGATATT+AGG	0.305787	5:-84851970	MsG0580028692.01.T01:CDS
TCATAAACTTGAGCATTATA+TGG	0.306286	5:+84852255	None:intergenic
AGGCGATACGGTAGGGGTTT+TGG	0.316658	5:+84854392	None:intergenic
CAATGCTTTCATCCAAATAC+TGG	0.327523	5:-84853444	MsG0580028692.01.T01:CDS
AGCTTGAAACAGCATCTTCT+TGG	0.329857	5:+84853784	None:intergenic
GATCATGATACATATGGTTT+AGG	0.333976	5:-84853564	MsG0580028692.01.T01:CDS
ATAGCAGACCAATGTGAATT+TGG	0.341801	5:-84852742	MsG0580028692.01.T01:CDS
AATATCCATCTTCATGCAAT+TGG	0.341818	5:+84854252	None:intergenic
ATGAAGGTAGATCGACTATC+TGG	0.349186	5:+84853849	None:intergenic
TTTGTTAGCAAATTTCTTCA+TGG	0.353735	5:+84853380	None:intergenic
ATGTCTCATTTCATTGGAAA+TGG	0.356476	5:-84853259	MsG0580028692.01.T01:CDS
CTTGATTTGCTAAGGTCTCT+TGG	0.362091	5:-84853216	MsG0580028692.01.T01:CDS
AAGGTCTCTTGGAAATTCCT+TGG	0.364902	5:-84853205	MsG0580028692.01.T01:CDS
TGCAAAATTGCAGTATTAGT+TGG	0.368307	5:+84853627	None:intergenic
TAATCAACAGTGACTCTGTT+TGG	0.369827	5:-84851933	MsG0580028692.01.T01:CDS
CCTAAACGCCGTTTCTTGAA+TGG	0.370619	5:+84851787	None:intergenic
CCATTCAAGAAACGGCGTTT+AGG	0.370861	5:-84851787	MsG0580028692.01.T01:CDS
AGGTCTCTTGGAAATTCCTT+GGG	0.371531	5:-84853204	MsG0580028692.01.T01:CDS
ACATTATTGATCTCACAAAA+AGG	0.373241	5:+84854607	None:intergenic
TGCTGATATCAAACTTATAT+GGG	0.374218	5:-84853736	MsG0580028692.01.T01:CDS
GCCAACATGTCTCATTTCAT+TGG	0.376355	5:-84853265	MsG0580028692.01.T01:CDS
CTTATAACAACTTGCAAAGT+TGG	0.382793	5:-84855598	MsG0580028692.01.T01:CDS
CATTTGTTGTCATGTTCATA+AGG	0.383088	5:+84854125	None:intergenic
TCATTGCTTTCACACACATT+AGG	0.388248	5:-84854444	MsG0580028692.01.T01:CDS
AATATTCATCTTCATGCATT+CGG	0.392803	5:+84854195	None:intergenic
TCACTTGATCATGATACATA+TGG	0.393125	5:-84853570	MsG0580028692.01.T01:CDS
TATCTGATGCTCCCATTGTC+AGG	0.393935	5:-84852600	MsG0580028692.01.T01:CDS
AAGAGTACATTTCTCCTTCA+AGG	0.396644	5:+84852554	None:intergenic
AGTTATTGATGCTAAGAATA+GGG	0.406528	5:-84851902	MsG0580028692.01.T01:CDS
ATATCTACCGTGAGATCTAA+TGG	0.406558	5:-84852126	MsG0580028692.01.T01:CDS
CATGTGTGAGGTTGATTTCA+GGG	0.407399	5:+84854087	None:intergenic
TGTTGGCATGTGTGTGTGTA+AGG	0.413125	5:+84853281	None:intergenic
ACTTTGCAAGTTGTTATAAG+AGG	0.418026	5:+84855601	None:intergenic
GCTTGAAACAGCATCTTCTT+GGG	0.426070	5:+84853785	None:intergenic
TAGTTATTGATGCTAAGAAT+AGG	0.429708	5:-84851903	MsG0580028692.01.T01:CDS
AAGTCTGAAGAACATGGTTC+AGG	0.431895	5:-84852479	MsG0580028692.01.T01:CDS
GTTGTCTCTCATCTCCGATT+AGG	0.431949	5:+84852872	None:intergenic
AATATAATTAAAACAGTGTT+GGG	0.435420	5:-84854064	MsG0580028692.01.T01:CDS
CTCCACCGTTGCATCCTAAT+CGG	0.437172	5:-84852886	MsG0580028692.01.T01:CDS
TCGGTTGCATAGGTTAGTTA+TGG	0.440947	5:-84853595	MsG0580028692.01.T01:CDS
GTGGCTGACTTAAACGTCTC+TGG	0.444543	5:+84854715	None:intergenic
GGAAATTATAAACCTGTGTC+AGG	0.444557	5:-84854472	MsG0580028692.01.T01:CDS
GCTAAGGGTAAAGAGCAATT+TGG	0.445056	5:-84852511	MsG0580028692.01.T01:CDS
CTTGATTACCTTCTTAACAT+AGG	0.451230	5:+84854372	None:intergenic
AGAAAGACATTGACAAGGCC+TGG	0.454988	5:+84853492	None:intergenic
TTCGCACGGGAATAAGCTAA+TGG	0.459341	5:+84852642	None:intergenic
TACAGTTCCATCATGACATA+TGG	0.461642	5:-84854534	MsG0580028692.01.T01:CDS
GACATTGTGCTATTTGGAAA+TGG	0.464393	5:-84853540	MsG0580028692.01.T01:CDS
ATATGGGGACCACCAGGGAC+AGG	0.468887	5:-84853720	MsG0580028692.01.T01:CDS
TAGCTTTCTTCCCTGACAAT+GGG	0.469265	5:+84852589	None:intergenic
GTTGGTCATGTTTACTTGAA+AGG	0.470464	5:-84855580	MsG0580028692.01.T01:intron
ATCATCCAATTGCATGAAGA+TGG	0.477710	5:-84854257	MsG0580028692.01.T01:CDS
GTTTGAAAGACTTGTAATGT+TGG	0.480465	5:-84852710	MsG0580028692.01.T01:CDS
AACAATTCCATTACAGCTGT+CGG	0.482727	5:-84852911	MsG0580028692.01.T01:CDS
TGGAGACCCGACAGCTGTAA+TGG	0.484216	5:+84852904	None:intergenic
GAATATAATTAAAACAGTGT+TGG	0.489127	5:-84854065	MsG0580028692.01.T01:CDS
TTCAAGCTCTTGATTTGCTA+AGG	0.490192	5:-84853224	MsG0580028692.01.T01:CDS
TGCTATTTGGAAATGGTAAG+AGG	0.490292	5:-84853533	MsG0580028692.01.T01:CDS
AGCTATGTTAATAAAAGAAT+AGG	0.495035	5:+84852531	None:intergenic
TTCAAGAAACGGCGTTTAGG+AGG	0.495052	5:-84851784	MsG0580028692.01.T01:CDS
AACAAATGTGGCTATGACTA+GGG	0.495248	5:-84852067	MsG0580028692.01.T01:CDS
GGAGTTCAGCAACTCTATCA+TGG	0.495440	5:+84853401	None:intergenic
ATAATTAAAAGTCTTTATGA+AGG	0.499892	5:-84852439	MsG0580028692.01.T01:intron
CATGATGGAACTGTATGAAT+TGG	0.504598	5:+84854542	None:intergenic
TTTGAAAGACTTGTAATGTT+GGG	0.506742	5:-84852709	MsG0580028692.01.T01:CDS
CGGCGTTTAGGAGGGAAAAT+GGG	0.506969	5:-84851775	MsG0580028692.01.T01:CDS
TTGAGTCACCATTCAAGAAA+CGG	0.508950	5:-84851795	MsG0580028692.01.T01:CDS
TTAACATAGGCGATACGGTA+GGG	0.510070	5:+84854385	None:intergenic
GAACAAATGTGGCTATGACT+AGG	0.512010	5:-84852068	MsG0580028692.01.T01:CDS
CTTTGCAAGTTGTTATAAGA+GGG	0.512013	5:+84855602	None:intergenic
TGTCAATGTTAAATTTAGGA+AGG	0.513940	5:+84853100	None:intergenic
CTTGAATTTGGCTTGCCCCA+AGG	0.515997	5:+84853188	None:intergenic
CCGTGCGAAGAGTTCTATGA+TGG	0.516329	5:-84852628	MsG0580028692.01.T01:CDS
ACCGTGAGATCTAATGGTAC+TGG	0.518270	5:-84852120	MsG0580028692.01.T01:CDS
CCGTATCGCCTATGTTAAGA+AGG	0.518702	5:-84854380	MsG0580028692.01.T01:CDS
CTATGTTAAGAAGGTAATCA+AGG	0.526997	5:-84854371	MsG0580028692.01.T01:CDS
AAGGTAATCAAGGCAAGGAA+TGG	0.530345	5:-84854361	MsG0580028692.01.T01:CDS
TCCAATGAAATGAGACATGT+TGG	0.531006	5:+84853264	None:intergenic
TCGAGGATGTTGTACTCTCA+TGG	0.532307	5:-84855350	MsG0580028692.01.T01:CDS
ACAATTCCATTACAGCTGTC+GGG	0.533970	5:-84852910	MsG0580028692.01.T01:CDS
CCTTCTTAACATAGGCGATA+CGG	0.534776	5:+84854380	None:intergenic
AAACTTATATGGGGACCACC+AGG	0.535882	5:-84853726	MsG0580028692.01.T01:CDS
ACAGAACATTCTTAATAGCC+CGG	0.537690	5:+84851842	None:intergenic
GTCAATGTTAAATTTAGGAA+GGG	0.543604	5:+84853101	None:intergenic
GTCATGCAAATGCATGAAGA+TGG	0.544816	5:-84854314	MsG0580028692.01.T01:CDS
TCTAATGGTACTGGTGATGT+TGG	0.546314	5:-84852111	MsG0580028692.01.T01:CDS
GCTTTCATCCAAATACTGGA+TGG	0.546705	5:-84853440	MsG0580028692.01.T01:CDS
AGGTGGCAATTCGGTTGCAT+AGG	0.549979	5:-84853605	MsG0580028692.01.T01:CDS
TTGTTTCCACAATGCTGAAG+AGG	0.550324	5:-84851878	MsG0580028692.01.T01:CDS
AGGAAATTGAGCAGTACACT+CGG	0.550497	5:-84852224	MsG0580028692.01.T01:CDS
ACATATCAACTACTTTCTCT+AGG	0.550836	5:-84851744	MsG0580028692.01.T01:CDS
ATAGCTTTCTTCCCTGACAA+TGG	0.550979	5:+84852588	None:intergenic
TGGAGCACAAGTCAATGTCC+TGG	0.557653	5:+84853647	None:intergenic
CGTTGAGAAAGAACGAAGGA+TGG	0.558853	5:-84855433	MsG0580028692.01.T01:CDS
TAACATAGGCGATACGGTAG+GGG	0.562252	5:+84854386	None:intergenic
AAAGTGAACTTAGTATGCTA+AGG	0.562963	5:+84853136	None:intergenic
TCTTCAGCCATATGTCATGA+TGG	0.567416	5:+84854527	None:intergenic
AAAAGAGTTCTTGTATTCTG+TGG	0.570824	5:+84854696	None:intergenic
TCAAGAAACGGCGTTTAGGA+GGG	0.570826	5:-84851783	MsG0580028692.01.T01:CDS
ATGTGGCTATGACTAGGGCT+AGG	0.571180	5:-84852062	MsG0580028692.01.T01:intron
CTTTCATCCAAATACTGGAT+GGG	0.573095	5:-84853439	MsG0580028692.01.T01:CDS
ATCGACTATCTGGTTCAGAC+AGG	0.573307	5:+84853859	None:intergenic
GGTAAAGAGCAATTTGGTCA+TGG	0.575475	5:-84852505	MsG0580028692.01.T01:CDS
ACAAGGAAGAAAGTTAGCAT+AGG	0.580771	5:-84852288	MsG0580028692.01.T01:CDS
CCATCATAGAACTCTTCGCA+CGG	0.582805	5:+84852628	None:intergenic
ACCAGTACCATTAGATCTCA+CGG	0.585010	5:+84852119	None:intergenic
AACCAGTAATGAAAGTGAAG+AGG	0.586546	5:-84855406	MsG0580028692.01.T01:CDS
ATCTGATGCTCCCATTGTCA+GGG	0.594382	5:-84852599	MsG0580028692.01.T01:CDS
CACGGGAATAAGCTAATGGA+AGG	0.595715	5:+84852646	None:intergenic
CAGAACATTCTTAATAGCCC+GGG	0.595796	5:+84851843	None:intergenic
TTTAATTATATTCATGTGTG+AGG	0.595989	5:+84854075	None:intergenic
CATCATAGAACTCTTCGCAC+GGG	0.599208	5:+84852629	None:intergenic
ACATTGACAAGGCCTGGATG+AGG	0.599408	5:+84853498	None:intergenic
TATGGGGACCACCAGGGACA+GGG	0.599903	5:-84853719	MsG0580028692.01.T01:CDS
ATGAAATTAAGTCCTCATCC+AGG	0.605229	5:-84853510	MsG0580028692.01.T01:CDS
TGAATATTGAAGATGACTTG+TGG	0.605497	5:-84854180	MsG0580028692.01.T01:CDS
CATCTCCGATTAGGATGCAA+CGG	0.610928	5:+84852881	None:intergenic
GAAACTAATTGATCCAGCAA+AGG	0.611704	5:-84852992	MsG0580028692.01.T01:CDS
TGTGTTTCCTCAAACAATAG+AGG	0.616517	5:+84854670	None:intergenic
TGCCAGTGTTCATGAAGACA+AGG	0.619804	5:-84852305	MsG0580028692.01.T01:intron
GGAAATTGAGCAGTACACTC+GGG	0.620554	5:-84852223	MsG0580028692.01.T01:CDS
TGAAGAGGATGTTCGACTCG+AGG	0.621652	5:-84855367	MsG0580028692.01.T01:CDS
TTAAGAAGGTAATCAAGGCA+AGG	0.623298	5:-84854366	MsG0580028692.01.T01:CDS
TTTGTTCAGAACATCCTCGA+GGG	0.625922	5:+84855326	None:intergenic
AAATTTAACATTGACATGAG+AGG	0.626648	5:-84853093	MsG0580028692.01.T01:CDS
TGTTAGCAATGCAAGTGAAG+AGG	0.630903	5:-84855382	MsG0580028692.01.T01:CDS
CTTAACATAGGCGATACGGT+AGG	0.632234	5:+84854384	None:intergenic
TACTTCGTCCAAATTCACAT+TGG	0.633182	5:+84852734	None:intergenic
TGGACAAAGTCTGAAGAACA+TGG	0.636091	5:-84852485	MsG0580028692.01.T01:CDS
AAATAGACAAAGAACAAATG+TGG	0.638430	5:-84852079	MsG0580028692.01.T01:CDS
TTGTAATGTTGGGATACGAA+AGG	0.638800	5:-84852699	MsG0580028692.01.T01:CDS
AACTTATATGGGGACCACCA+GGG	0.643082	5:-84853725	MsG0580028692.01.T01:CDS
AACTACTTTCTCTAGGCGAG+TGG	0.643303	5:-84851737	MsG0580028692.01.T01:CDS
GCTGATATCAAACTTATATG+GGG	0.644417	5:-84853735	MsG0580028692.01.T01:CDS
AGATGACTTGCAGAACAACA+AGG	0.652641	5:-84854284	MsG0580028692.01.T01:CDS
GCAATGAGATCTCCTGACAC+AGG	0.658736	5:+84854460	None:intergenic
GGTCTCTTGGAAATTCCTTG+GGG	0.661476	5:-84853203	MsG0580028692.01.T01:CDS
AGTATTCTTACAATTGGACG+CGG	0.662302	5:+84854040	None:intergenic
TCAAGTTTATGAAATTCAGG+AGG	0.663310	5:-84852244	MsG0580028692.01.T01:CDS
ATTGAATGAGATTCTCTACA+CGG	0.664980	5:+84853463	None:intergenic
CTCCGATTAGGATGCAACGG+TGG	0.685534	5:+84852884	None:intergenic
TTAGCGTTGAGAAAGAACGA+AGG	0.716168	5:-84855437	MsG0580028692.01.T01:intron
TGTACTCTCATGGACCCTCG+AGG	0.722293	5:-84855340	MsG0580028692.01.T01:CDS
TATCGAGAAAGACATTGACA+AGG	0.728740	5:+84853487	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATAAGCAATTAATCATTA+AGG	+	Chr5:84851877-84851896	None:intergenic	15.0%
!!	AATATAATTAAAACAGTGTT+GGG	-	Chr5:84853273-84853292	MsG0580028692.01.T01:CDS	15.0%
!!	ATAATTAAAAGTCTTTATGA+AGG	-	Chr5:84854898-84854917	MsG0580028692.01.T01:intron	15.0%
!!	GTTAATTTATATGATGTTTA+TGG	-	Chr5:84852276-84852295	MsG0580028692.01.T01:CDS	15.0%
!!	TAAAAGATGATGTAATTAAT+TGG	+	Chr5:84852318-84852337	None:intergenic	15.0%
!!!	AAAGTTATTTTTCTTGAATT+TGG	+	Chr5:84854164-84854183	None:intergenic	15.0%
!!!	ATAAAGATTGAGTATTTTTA+TGG	-	Chr5:84852126-84852145	MsG0580028692.01.T01:CDS	15.0%
!!!	TTTTACAAACTAAAACAAAA+TGG	+	Chr5:84852395-84852414	None:intergenic	15.0%
!!!	TTTTAGTTTGTAAAATAGTA+TGG	-	Chr5:84852400-84852419	MsG0580028692.01.T01:intron	15.0%
!!	AGCTATGTTAATAAAAGAAT+AGG	+	Chr5:84854809-84854828	None:intergenic	20.0%
!!	GAATATAATTAAAACAGTGT+TGG	-	Chr5:84853272-84853291	MsG0580028692.01.T01:CDS	20.0%
!!	GTATGCTATATATTAAATGA+AGG	+	Chr5:84853345-84853364	None:intergenic	20.0%
!!	TTTAATTATATTCATGTGTG+AGG	+	Chr5:84853265-84853284	None:intergenic	20.0%
!!	TTTGAATACAAGAAGATATT+TGG	-	Chr5:84853998-84854017	MsG0580028692.01.T01:intron	20.0%
!!!	AATACAAAAAAGCATTAAAC+AGG	+	Chr5:84853431-84853450	None:intergenic	20.0%
!!!	TCTTTTATTAACATAGCTAA+GGG	-	Chr5:84854811-84854830	MsG0580028692.01.T01:intron	20.0%
!!!	TTCTTTTATTAACATAGCTA+AGG	-	Chr5:84854810-84854829	MsG0580028692.01.T01:intron	20.0%
!	AAATAGACAAAGAACAAATG+TGG	-	Chr5:84855258-84855277	MsG0580028692.01.T01:intron	25.0%
!	AAATTTAACATTGACATGAG+AGG	-	Chr5:84854244-84854263	MsG0580028692.01.T01:CDS	25.0%
!	AACCATTATGTTGTATTTCA+TGG	-	Chr5:84854941-84854960	MsG0580028692.01.T01:intron	25.0%
!	AATATTCATCTTCATGCATT+CGG	+	Chr5:84853145-84853164	None:intergenic	25.0%
!	ACATTATTGATCTCACAAAA+AGG	+	Chr5:84852733-84852752	None:intergenic	25.0%
!	AGTTATTGATGCTAAGAATA+GGG	-	Chr5:84855435-84855454	MsG0580028692.01.T01:CDS	25.0%
!	ATAATGTTCAAAAAACTGCA+TGG	-	Chr5:84852746-84852765	MsG0580028692.01.T01:intron	25.0%
!	ATGCTGATATCAAACTTATA+TGG	-	Chr5:84853600-84853619	MsG0580028692.01.T01:CDS	25.0%
!	CAAAAATCTTTACAAAGACA+AGG	-	Chr5:84852030-84852049	MsG0580028692.01.T01:intron	25.0%
!	CATTGTAAAGAATCTAAAGA+TGG	+	Chr5:84852063-84852082	None:intergenic	25.0%
!	CTCATGTCAATGTTAAATTT+AGG	+	Chr5:84854244-84854263	None:intergenic	25.0%
!	GTCAATGTTAAATTTAGGAA+GGG	+	Chr5:84854239-84854258	None:intergenic	25.0%
!	TAGTTATTGATGCTAAGAAT+AGG	-	Chr5:84855434-84855453	MsG0580028692.01.T01:CDS	25.0%
!	TCATAAACTTGAGCATTATA+TGG	+	Chr5:84855085-84855104	None:intergenic	25.0%
!	TCTGATGAAGTTGAAAATAT+TGG	-	Chr5:84852844-84852863	MsG0580028692.01.T01:intron	25.0%
!	TGCTGATATCAAACTTATAT+GGG	-	Chr5:84853601-84853620	MsG0580028692.01.T01:CDS	25.0%
!	TGTCAATGTTAAATTTAGGA+AGG	+	Chr5:84854240-84854259	None:intergenic	25.0%
!	TTCCATGAAATACAACATAA+TGG	+	Chr5:84854946-84854965	None:intergenic	25.0%
!	TTTGAAAGACTTGTAATGTT+GGG	-	Chr5:84854628-84854647	MsG0580028692.01.T01:CDS	25.0%
!	TTTGTTAGCAAATTTCTTCA+TGG	+	Chr5:84853960-84853979	None:intergenic	25.0%
!!	AATACTCTTTTGATCTGAAT+AGG	-	Chr5:84853314-84853333	MsG0580028692.01.T01:CDS	25.0%
!!	TATAATAAGCTTTTCCTTGA+AGG	-	Chr5:84854769-84854788	MsG0580028692.01.T01:intron	25.0%
!!	TATCATGTCTTCTTTTATGA+AGG	+	Chr5:84853507-84853526	None:intergenic	25.0%
!!	TCTTTGTGTTTTCGTTAATT+TGG	-	Chr5:84851829-84851848	MsG0580028692.01.T01:CDS	25.0%
!!	TTCAAACGTAATTTTTCATG+CGG	+	Chr5:84853373-84853392	None:intergenic	25.0%
!!!	ATGAATTGGTTTTGATCTTT+TGG	+	Chr5:84852784-84852803	None:intergenic	25.0%
!!!	CAAAAGAGTATTCTTACAAT+TGG	+	Chr5:84853306-84853325	None:intergenic	25.0%
	AAAAGAGTTCTTGTATTCTG+TGG	+	Chr5:84852644-84852663	None:intergenic	30.0%
	AAAGTGAACTTAGTATGCTA+AGG	+	Chr5:84854204-84854223	None:intergenic	30.0%
	AAGTGCATTGCATTGTTAAA+AGG	+	Chr5:84852436-84852455	None:intergenic	30.0%
	AATATCCATCTTCATGCAAT+TGG	+	Chr5:84853088-84853107	None:intergenic	30.0%
	AATCTTTATTGAGACTGGTA+TGG	+	Chr5:84852115-84852134	None:intergenic	30.0%
	ACATATCAACTACTTTCTCT+AGG	-	Chr5:84855593-84855612	MsG0580028692.01.T01:CDS	30.0%
	ACTTTGCAAGTTGTTATAAG+AGG	+	Chr5:84851739-84851758	None:intergenic	30.0%
	AGATACTGTCTTTGGATATT+AGG	-	Chr5:84855367-84855386	MsG0580028692.01.T01:CDS	30.0%
	ATGTCTCATTTCATTGGAAA+TGG	-	Chr5:84854078-84854097	MsG0580028692.01.T01:CDS	30.0%
	ATTGAATGAGATTCTCTACA+CGG	+	Chr5:84853877-84853896	None:intergenic	30.0%
	CTATGTTAAGAAGGTAATCA+AGG	-	Chr5:84852966-84852985	MsG0580028692.01.T01:CDS	30.0%
	CTCTAACTGTTTAGTTACTT+AGG	-	Chr5:84851794-84851813	MsG0580028692.01.T01:CDS	30.0%
	CTTATAACAACTTGCAAAGT+TGG	-	Chr5:84851739-84851758	MsG0580028692.01.T01:CDS	30.0%
	CTTGATTACCTTCTTAACAT+AGG	+	Chr5:84852968-84852987	None:intergenic	30.0%
	CTTTGCAAGTTGTTATAAGA+GGG	+	Chr5:84851738-84851757	None:intergenic	30.0%
	GATCATGATACATATGGTTT+AGG	-	Chr5:84853773-84853792	MsG0580028692.01.T01:CDS	30.0%
	GCTGATATCAAACTTATATG+GGG	-	Chr5:84853602-84853621	MsG0580028692.01.T01:CDS	30.0%
	GTTTGAAAGACTTGTAATGT+TGG	-	Chr5:84854627-84854646	MsG0580028692.01.T01:CDS	30.0%
	TACTCAATCTTTATTGAGAC+TGG	+	Chr5:84852120-84852139	None:intergenic	30.0%
	TCAAGTTTATGAAATTCAGG+AGG	-	Chr5:84855093-84855112	MsG0580028692.01.T01:intron	30.0%
	TCACTTGATCATGATACATA+TGG	-	Chr5:84853767-84853786	MsG0580028692.01.T01:CDS	30.0%
	TCGATTACTAAAAATTGCAC+TGG	+	Chr5:84854376-84854395	None:intergenic	30.0%
	TGAATATTGAAGATGACTTG+TGG	-	Chr5:84853157-84853176	MsG0580028692.01.T01:CDS	30.0%
	TGCAAAATTGCAGTATTAGT+TGG	+	Chr5:84853713-84853732	None:intergenic	30.0%
	TGCTCAAGTTTATGAAATTC+AGG	-	Chr5:84855090-84855109	MsG0580028692.01.T01:intron	30.0%
	TTCAAGTCATTTAAGCTTTC+AGG	+	Chr5:84852919-84852938	None:intergenic	30.0%
!	AACTAATACTGCAATTTTGC+AGG	-	Chr5:84853712-84853731	MsG0580028692.01.T01:CDS	30.0%
!	TCAAACGTAATTTTTCATGC+GGG	+	Chr5:84853372-84853391	None:intergenic	30.0%
!	TTAGTAAAAAGCAAGGTACT+CGG	-	Chr5:84854496-84854515	MsG0580028692.01.T01:CDS	30.0%
!	TTTTGTACCTCTATTGTTTG+AGG	-	Chr5:84852660-84852679	MsG0580028692.01.T01:CDS	30.0%
!!	CATTTGTTGTCATGTTCATA+AGG	+	Chr5:84853215-84853234	None:intergenic	30.0%
!!!	TGCTTTTTTGTATTTCAGAG+TGG	-	Chr5:84853437-84853456	MsG0580028692.01.T01:CDS	30.0%
	AACAAATGTGGCTATGACTA+GGG	-	Chr5:84855270-84855289	MsG0580028692.01.T01:intron	35.0%
	AACAATTCCATTACAGCTGT+CGG	-	Chr5:84854426-84854445	MsG0580028692.01.T01:CDS	35.0%
	AACCAGTAATGAAAGTGAAG+AGG	-	Chr5:84851931-84851950	MsG0580028692.01.T01:CDS	35.0%
	ACAAGGAAGAAAGTTAGCAT+AGG	-	Chr5:84855049-84855068	MsG0580028692.01.T01:intron	35.0%
	ACAGAACATTCTTAATAGCC+CGG	+	Chr5:84855498-84855517	None:intergenic	35.0%
	AGTATTCTTACAATTGGACG+CGG	+	Chr5:84853300-84853319	None:intergenic	35.0%
	ATAGCAGACCAATGTGAATT+TGG	-	Chr5:84854595-84854614	MsG0580028692.01.T01:CDS	35.0%
	ATATCTACCGTGAGATCTAA+TGG	-	Chr5:84855211-84855230	MsG0580028692.01.T01:intron	35.0%
	ATCATCCAATTGCATGAAGA+TGG	-	Chr5:84853080-84853099	MsG0580028692.01.T01:CDS	35.0%
	ATCCTCTTCACTTTCATTAC+TGG	+	Chr5:84851936-84851955	None:intergenic	35.0%
	ATGAAATTAAGTCCTCATCC+AGG	-	Chr5:84853827-84853846	MsG0580028692.01.T01:CDS	35.0%
	ATTCTTTCAGTCGTTGAATC+TGG	+	Chr5:84855522-84855541	None:intergenic	35.0%
	CAATGCTTTCATCCAAATAC+TGG	-	Chr5:84853893-84853912	MsG0580028692.01.T01:intron	35.0%
	CTCTACTCAAGTTAAAAACC+AGG	-	Chr5:84853672-84853691	MsG0580028692.01.T01:CDS	35.0%
	CTTTCATCCAAATACTGGAT+GGG	-	Chr5:84853898-84853917	MsG0580028692.01.T01:intron	35.0%
	GAAACTAATTGATCCAGCAA+AGG	-	Chr5:84854345-84854364	MsG0580028692.01.T01:CDS	35.0%
	GGAAATTATAAACCTGTGTC+AGG	-	Chr5:84852865-84852884	MsG0580028692.01.T01:CDS	35.0%
	GTAAATGTTCGTTCTGTTGA+TGG	-	Chr5:84855157-84855176	MsG0580028692.01.T01:intron	35.0%
	GTTGGTCATGTTTACTTGAA+AGG	-	Chr5:84851757-84851776	MsG0580028692.01.T01:CDS	35.0%
	TAATCAACAGTGACTCTGTT+TGG	-	Chr5:84855404-84855423	MsG0580028692.01.T01:CDS	35.0%
	TACAGTTCCATCATGACATA+TGG	-	Chr5:84852803-84852822	MsG0580028692.01.T01:intron	35.0%
	TACTTCGTCCAAATTCACAT+TGG	+	Chr5:84854606-84854625	None:intergenic	35.0%
	TATCGAGAAAGACATTGACA+AGG	+	Chr5:84853853-84853872	None:intergenic	35.0%
	TCATTGCTTTCACACACATT+AGG	-	Chr5:84852893-84852912	MsG0580028692.01.T01:CDS	35.0%
	TCCAATGAAATGAGACATGT+TGG	+	Chr5:84854076-84854095	None:intergenic	35.0%
	TCCTGCATTAGTAAAAAGCA+AGG	-	Chr5:84854489-84854508	MsG0580028692.01.T01:CDS	35.0%
	TGCTGAAGAGGACAAAAAAT+TGG	-	Chr5:84855471-84855490	MsG0580028692.01.T01:intron	35.0%
	TGTGTTTCCTCAAACAATAG+AGG	+	Chr5:84852670-84852689	None:intergenic	35.0%
	TTAAGAAGGTAATCAAGGCA+AGG	-	Chr5:84852971-84852990	MsG0580028692.01.T01:CDS	35.0%
	TTGAGTCACCATTCAAGAAA+CGG	-	Chr5:84855542-84855561	MsG0580028692.01.T01:intron	35.0%
	TTGTAATGTTGGGATACGAA+AGG	-	Chr5:84854638-84854657	MsG0580028692.01.T01:CDS	35.0%
!	AAGAGTACATTTCTCCTTCA+AGG	+	Chr5:84854786-84854805	None:intergenic	35.0%
!	CATGATGGAACTGTATGAAT+TGG	+	Chr5:84852798-84852817	None:intergenic	35.0%
!	TAATACTGCAATTTTGCAGG+TGG	-	Chr5:84853715-84853734	MsG0580028692.01.T01:CDS	35.0%
!	TACAATGTGCACTTGTTTCT+TGG	-	Chr5:84852076-84852095	MsG0580028692.01.T01:CDS	35.0%
!	TAGTAAAAAGCAAGGTACTC+GGG	-	Chr5:84854497-84854516	MsG0580028692.01.T01:CDS	35.0%
!	TGCTATTTGGAAATGGTAAG+AGG	-	Chr5:84853804-84853823	MsG0580028692.01.T01:CDS	35.0%
!	TTAGGTGACATTGTGCTATT+TGG	-	Chr5:84853791-84853810	MsG0580028692.01.T01:CDS	35.0%
!	TTTTGTGCAGATACTGTCTT+TGG	-	Chr5:84855359-84855378	MsG0580028692.01.T01:CDS	35.0%
!!	GACAGTACTGAGATTTTATC+AGG	+	Chr5:84853006-84853025	None:intergenic	35.0%
!!	GACATTGTGCTATTTGGAAA+TGG	-	Chr5:84853797-84853816	MsG0580028692.01.T01:CDS	35.0%
!!	TTCAAGCTCTTGATTTGCTA+AGG	-	Chr5:84854113-84854132	MsG0580028692.01.T01:CDS	35.0%
!!!	ACCTTGCTTTTTACTAATGC+AGG	+	Chr5:84854493-84854512	None:intergenic	35.0%
	AAGAGGACAAAAAATTGGCC+CGG	-	Chr5:84855476-84855495	MsG0580028692.01.T01:intron	40.0%
	AAGGTAATCAAGGCAAGGAA+TGG	-	Chr5:84852976-84852995	MsG0580028692.01.T01:CDS	40.0%
	AAGGTCTCTTGGAAATTCCT+TGG	-	Chr5:84854132-84854151	MsG0580028692.01.T01:CDS	40.0%
	AAGTCTGAAGAACATGGTTC+AGG	-	Chr5:84854858-84854877	MsG0580028692.01.T01:intron	40.0%
	ACAATTCCATTACAGCTGTC+GGG	-	Chr5:84854427-84854446	MsG0580028692.01.T01:CDS	40.0%
	ACCAGTACCATTAGATCTCA+CGG	+	Chr5:84855221-84855240	None:intergenic	40.0%
	AGCTTGAAACAGCATCTTCT+TGG	+	Chr5:84853556-84853575	None:intergenic	40.0%
	AGGAAATTGAGCAGTACACT+CGG	-	Chr5:84855113-84855132	MsG0580028692.01.T01:intron	40.0%
	AGGTCTCTTGGAAATTCCTT+GGG	-	Chr5:84854133-84854152	MsG0580028692.01.T01:CDS	40.0%
	ATACGAACGCTGAAGATTTC+TGG	+	Chr5:84854969-84854988	None:intergenic	40.0%
	ATAGCTTTCTTCCCTGACAA+TGG	+	Chr5:84854752-84854771	None:intergenic	40.0%
	ATGAAGGTAGATCGACTATC+TGG	+	Chr5:84853491-84853510	None:intergenic	40.0%
	CAGAACATTCTTAATAGCCC+GGG	+	Chr5:84855497-84855516	None:intergenic	40.0%
	CATATGCGAGACTACATAGA+AGG	+	Chr5:84852180-84852199	None:intergenic	40.0%
	CCTTCTTAACATAGGCGATA+CGG	+	Chr5:84852960-84852979	None:intergenic	40.0%
	GAAAAAACCTATGTTCTCGC+AGG	-	Chr5:84852588-84852607	MsG0580028692.01.T01:CDS	40.0%
	GAACAAATGTGGCTATGACT+AGG	-	Chr5:84855269-84855288	MsG0580028692.01.T01:intron	40.0%
	GCCAACATGTCTCATTTCAT+TGG	-	Chr5:84854072-84854091	MsG0580028692.01.T01:CDS	40.0%
	GCTAAGGGTAAAGAGCAATT+TGG	-	Chr5:84854826-84854845	MsG0580028692.01.T01:intron	40.0%
	GCTTGAAACAGCATCTTCTT+GGG	+	Chr5:84853555-84853574	None:intergenic	40.0%
	GCTTTCATCCAAATACTGGA+TGG	-	Chr5:84853897-84853916	MsG0580028692.01.T01:intron	40.0%
	GGTAAAGAGCAATTTGGTCA+TGG	-	Chr5:84854832-84854851	MsG0580028692.01.T01:intron	40.0%
	GTCATGCAAATGCATGAAGA+TGG	-	Chr5:84853023-84853042	MsG0580028692.01.T01:CDS	40.0%
	TAGCTTTCTTCCCTGACAAT+GGG	+	Chr5:84854751-84854770	None:intergenic	40.0%
	TCTTCAGCCATATGTCATGA+TGG	+	Chr5:84852813-84852832	None:intergenic	40.0%
	TGGACAAAGTCTGAAGAACA+TGG	-	Chr5:84854852-84854871	MsG0580028692.01.T01:intron	40.0%
	TGTTAGCAATGCAAGTGAAG+AGG	-	Chr5:84851955-84851974	MsG0580028692.01.T01:CDS	40.0%
	TTAACATAGGCGATACGGTA+GGG	+	Chr5:84852955-84852974	None:intergenic	40.0%
	TTAGTCTCCCATCCAGTATT+TGG	+	Chr5:84853908-84853927	None:intergenic	40.0%
	TTATGAACCTGCGAGAACAT+AGG	+	Chr5:84852598-84852617	None:intergenic	40.0%
	TTCCTTGTCTTCATGAACAC+TGG	+	Chr5:84855037-84855056	None:intergenic	40.0%
	TTGTTTCCACAATGCTGAAG+AGG	-	Chr5:84855459-84855478	MsG0580028692.01.T01:intron	40.0%
	TTTGTTCAGAACATCCTCGA+GGG	+	Chr5:84852014-84852033	None:intergenic	40.0%
!	AGATGACTTGCAGAACAACA+AGG	-	Chr5:84853053-84853072	MsG0580028692.01.T01:CDS	40.0%
!	CAATTTTGCAGGTGGCAATT+CGG	-	Chr5:84853723-84853742	MsG0580028692.01.T01:CDS	40.0%
!	CATGTGTGAGGTTGATTTCA+GGG	+	Chr5:84853253-84853272	None:intergenic	40.0%
!	TCATGTGTGAGGTTGATTTC+AGG	+	Chr5:84853254-84853273	None:intergenic	40.0%
!	TCGGTTGCATAGGTTAGTTA+TGG	-	Chr5:84853742-84853761	MsG0580028692.01.T01:CDS	40.0%
!	TTAGCGTTGAGAAAGAACGA+AGG	-	Chr5:84851900-84851919	MsG0580028692.01.T01:CDS	40.0%
!	TTTTGTCCTCTTCAGCATTG+TGG	+	Chr5:84855468-84855487	None:intergenic	40.0%
!	TTTTGTTCAGAACATCCTCG+AGG	+	Chr5:84852015-84852034	None:intergenic	40.0%
!!	CTTGATTTGCTAAGGTCTCT+TGG	-	Chr5:84854121-84854140	MsG0580028692.01.T01:CDS	40.0%
!!	TCTAATGGTACTGGTGATGT+TGG	-	Chr5:84855226-84855245	MsG0580028692.01.T01:intron	40.0%
!!!	CGTTCTGTTGATGGTTTTCA+AGG	-	Chr5:84855166-84855185	MsG0580028692.01.T01:intron	40.0%
!!!	TCTGTTGATGGTTTTCAAGG+TGG	-	Chr5:84855169-84855188	MsG0580028692.01.T01:intron	40.0%
	AAACTTATATGGGGACCACC+AGG	-	Chr5:84853611-84853630	MsG0580028692.01.T01:CDS	45.0%
	AACTACTTTCTCTAGGCGAG+TGG	-	Chr5:84855600-84855619	MsG0580028692.01.T01:CDS	45.0%
	AACTTATATGGGGACCACCA+GGG	-	Chr5:84853612-84853631	MsG0580028692.01.T01:CDS	45.0%
	ACCGTGAGATCTAATGGTAC+TGG	-	Chr5:84855217-84855236	MsG0580028692.01.T01:intron	45.0%
	AGAAAGACATTGACAAGGCC+TGG	+	Chr5:84853848-84853867	None:intergenic	45.0%
	AGAGGACAAAAAATTGGCCC+GGG	-	Chr5:84855477-84855496	MsG0580028692.01.T01:intron	45.0%
	ATCTGATGCTCCCATTGTCA+GGG	-	Chr5:84854738-84854757	MsG0580028692.01.T01:intron	45.0%
	CACGGGAATAAGCTAATGGA+AGG	+	Chr5:84854694-84854713	None:intergenic	45.0%
	CATCATAGAACTCTTCGCAC+GGG	+	Chr5:84854711-84854730	None:intergenic	45.0%
	CATCTCCGATTAGGATGCAA+CGG	+	Chr5:84854459-84854478	None:intergenic	45.0%
	CCATCATAGAACTCTTCGCA+CGG	+	Chr5:84854712-84854731	None:intergenic	45.0%
	CCATTCAAGAAACGGCGTTT+AGG	-	Chr5:84855550-84855569	MsG0580028692.01.T01:intron	45.0%
	CCGTATCGCCTATGTTAAGA+AGG	-	Chr5:84852957-84852976	MsG0580028692.01.T01:CDS	45.0%
	CCTAAACGCCGTTTCTTGAA+TGG	+	Chr5:84855553-84855572	None:intergenic	45.0%
	CGTTGAGAAAGAACGAAGGA+TGG	-	Chr5:84851904-84851923	MsG0580028692.01.T01:CDS	45.0%
	CTTAACATAGGCGATACGGT+AGG	+	Chr5:84852956-84852975	None:intergenic	45.0%
	GGAAATTGAGCAGTACACTC+GGG	-	Chr5:84855114-84855133	MsG0580028692.01.T01:intron	45.0%
	GGAGTTCAGCAACTCTATCA+TGG	+	Chr5:84853939-84853958	None:intergenic	45.0%
	GGTCTCTTGGAAATTCCTTG+GGG	-	Chr5:84854134-84854153	MsG0580028692.01.T01:CDS	45.0%
	GTTGTCTCTCATCTCCGATT+AGG	+	Chr5:84854468-84854487	None:intergenic	45.0%
	TAACATAGGCGATACGGTAG+GGG	+	Chr5:84852954-84852973	None:intergenic	45.0%
	TATCTGATGCTCCCATTGTC+AGG	-	Chr5:84854737-84854756	MsG0580028692.01.T01:intron	45.0%
	TCAAGAAACGGCGTTTAGGA+GGG	-	Chr5:84855554-84855573	MsG0580028692.01.T01:intron	45.0%
	TCGAGGATGTTGTACTCTCA+TGG	-	Chr5:84851987-84852006	MsG0580028692.01.T01:intron	45.0%
	TGCCAGTGTTCATGAAGACA+AGG	-	Chr5:84855032-84855051	MsG0580028692.01.T01:intron	45.0%
	TGTTGGCATGTGTGTGTGTA+AGG	+	Chr5:84854059-84854078	None:intergenic	45.0%
	TTCAAGAAACGGCGTTTAGG+AGG	-	Chr5:84855553-84855572	MsG0580028692.01.T01:intron	45.0%
	TTCGCACGGGAATAAGCTAA+TGG	+	Chr5:84854698-84854717	None:intergenic	45.0%
!	ATCGACTATCTGGTTCAGAC+AGG	+	Chr5:84853481-84853500	None:intergenic	45.0%
!!	TGATGGTTTTCAAGGTGGTG+AGG	-	Chr5:84855174-84855193	MsG0580028692.01.T01:intron	45.0%
	ACATTGACAAGGCCTGGATG+AGG	+	Chr5:84853842-84853861	None:intergenic	50.0%
	ACGGCGTTTAGGAGGGAAAA+TGG	-	Chr5:84855561-84855580	MsG0580028692.01.T01:intron	50.0%
	AGGTGGCAATTCGGTTGCAT+AGG	-	Chr5:84853732-84853751	MsG0580028692.01.T01:CDS	50.0%
	ATGTGGCTATGACTAGGGCT+AGG	-	Chr5:84855275-84855294	MsG0580028692.01.T01:intron	50.0%
	CCGTGCGAAGAGTTCTATGA+TGG	-	Chr5:84854709-84854728	MsG0580028692.01.T01:CDS	50.0%
	CGGCGTTTAGGAGGGAAAAT+GGG	-	Chr5:84855562-84855581	MsG0580028692.01.T01:intron	50.0%
	CTCCACCGTTGCATCCTAAT+CGG	-	Chr5:84854451-84854470	MsG0580028692.01.T01:CDS	50.0%
	GCAATGAGATCTCCTGACAC+AGG	+	Chr5:84852880-84852899	None:intergenic	50.0%
	TGGAGCACAAGTCAATGTCC+TGG	+	Chr5:84853693-84853712	None:intergenic	50.0%
!	GTGGCTGACTTAAACGTCTC+TGG	+	Chr5:84852625-84852644	None:intergenic	50.0%
!	TGAAGAGGATGTTCGACTCG+AGG	-	Chr5:84851970-84851989	MsG0580028692.01.T01:CDS	50.0%
!!	CTTGAATTTGGCTTGCCCCA+AGG	+	Chr5:84854152-84854171	None:intergenic	50.0%
!!	TTTGTTTTCCCTGTCCCTGG+TGG	+	Chr5:84853629-84853648	None:intergenic	50.0%
!!!	GTCTTTGTTTTCCCTGTCCC+TGG	+	Chr5:84853632-84853651	None:intergenic	50.0%
	CTCCGATTAGGATGCAACGG+TGG	+	Chr5:84854456-84854475	None:intergenic	55.0%
	TGGAGACCCGACAGCTGTAA+TGG	+	Chr5:84854436-84854455	None:intergenic	55.0%
	TGTACTCTCATGGACCCTCG+AGG	-	Chr5:84851997-84852016	MsG0580028692.01.T01:intron	55.0%
!	AGGCGATACGGTAGGGGTTT+TGG	+	Chr5:84852948-84852967	None:intergenic	55.0%
!!	TGCACTGGCTTCACCTTTGC+TGG	+	Chr5:84854361-84854380	None:intergenic	55.0%
	ATATGGGGACCACCAGGGAC+AGG	-	Chr5:84853617-84853636	MsG0580028692.01.T01:CDS	60.0%
	TATGGGGACCACCAGGGACA+GGG	-	Chr5:84853618-84853637	MsG0580028692.01.T01:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	84851726	84855633	84851726	ID=MsG0580028692.01;Name=MsG0580028692.01
Chr5	mRNA	84851726	84855633	84851726	ID=MsG0580028692.01.T01;Parent=MsG0580028692.01;Name=MsG0580028692.01.T01;_AED=0.05;_eAED=0.05;_QI=0\|0\|0\|1\|0.83\|0.85\|7\|0\|934
Chr5	exon	84855581	84855633	84855581	ID=MsG0580028692.01.T01:exon:5981;Parent=MsG0580028692.01.T01
Chr5	exon	84855308	84855455	84855308	ID=MsG0580028692.01.T01:exon:5980;Parent=MsG0580028692.01.T01
Chr5	exon	84854051	84854749	84854051	ID=MsG0580028692.01.T01:exon:5979;Parent=MsG0580028692.01.T01
Chr5	exon	84852849	84853904	84852849	ID=MsG0580028692.01.T01:exon:5978;Parent=MsG0580028692.01.T01
Chr5	exon	84852440	84852764	84852440	ID=MsG0580028692.01.T01:exon:5977;Parent=MsG0580028692.01.T01
Chr5	exon	84852063	84852321	84852063	ID=MsG0580028692.01.T01:exon:5976;Parent=MsG0580028692.01.T01
Chr5	exon	84851726	84851990	84851726	ID=MsG0580028692.01.T01:exon:5975;Parent=MsG0580028692.01.T01
Chr5	CDS	84855581	84855633	84855581	ID=MsG0580028692.01.T01:cds;Parent=MsG0580028692.01.T01
Chr5	CDS	84855308	84855455	84855308	ID=MsG0580028692.01.T01:cds;Parent=MsG0580028692.01.T01
Chr5	CDS	84854051	84854749	84854051	ID=MsG0580028692.01.T01:cds;Parent=MsG0580028692.01.T01
Chr5	CDS	84852849	84853904	84852849	ID=MsG0580028692.01.T01:cds;Parent=MsG0580028692.01.T01
Chr5	CDS	84852440	84852764	84852440	ID=MsG0580028692.01.T01:cds;Parent=MsG0580028692.01.T01
Chr5	CDS	84852063	84852321	84852063	ID=MsG0580028692.01.T01:cds;Parent=MsG0580028692.01.T01
Chr5	CDS	84851726	84851990	84851726	ID=MsG0580028692.01.T01:cds;Parent=MsG0580028692.01.T01

Gene Sequence

>MsG0580028692.01.T01

ATGTTTGTTCCCTCTTATAACAACTTGCAAAGTTGGTCATGTTTACTTGAAAGCGTTGAGAAAGAACGAAGGATGGAAACAACCAGTAATGAAAGTGAAGAGGATGTTAGCAATGCAAGTGAAGAGGATGTTCGACTCGAGGATGTTGTACTCTCATGGACCCTCGAGGATGTTCTGAACAAAAATCTTTACAAAGACAAGGTTCATAAGATTCCAGAGACGTTTAAGTCAGCCACAGAATACAAGAACTCTTTTGTACCTCTATTGTTTGAGGAAACACACACTGATTTATCTTCAAGCTTGTTTGCTGTTTCTCAAGCTCCTTTTTGTGAGATCAATAATGTTCAAAAAACTGCATGGAAACTTTCAATTCCAAAAGATCAAAACCAATTCATACAGTTCCATCATGACATATGGCTGAAGAGTACAACTGAATCTGATGAAGTTGAAAATATTGGAAATTATAAACCTGTGTCAGGAGATCTCATTGCTTTCACACACATTAGGCCTGAAAGCTTAAATGACTTGAACACACACCAAAACCCCTACCGTATCGCCTATGTTAAGAAGGTAATCAAGGCAAGGAATGGAATTCCTGATAAAATCTCAGTACTGTCATGCAAATGCATGAAGATGGATATCAAAGATGACTTGCAGAACAACAAGGAGCTATCATCCAATTGCATGAAGATGGATATTGAAGACGACTTGTGTAACAACAAAGAGCTAGCATCCGAATGCATGAAGATGAATATTGAAGATGACTTGTGGAAGAACAAAGAGTTGAAACTGTATGCAGTTTACCTTATGAACATGACAACAAATGTTCGTATTTGCAATGCCCTGAAATCAACCTCACACATGAATATAATTAAAACAGTGTTGGGACCGCGTCCAATTAGTGGAGAAAACTGTCAACTTTGCCTGTCTGAACCAGATAGTCGATCTACCTTCATAAAAGAAGACATGATAATTCGTTCTCAGAATCTAAATGAATCCCAAGAAGATGCTGTTTCAAGCTGTGTTAGCATGATGAATTGCAATCATGCTGATATCAAACTTATATGGGGACCACCAGGGACAGGGAAAACAAAGACAGTTGCATGCTTGCTATTTTCTCTACTCAAGTTAAAAACCAGGACATTGACTTGTGCTCCAACTAATACTGCAATTTTGCAGGTGGCAATTCGGTTGCATAGGTTAGTTATGGATTCACTTGATCATGATACATATGGTTTAGGTGACATTGTGCTATTTGGAAATGGTAAGAGGATGAAATTAAGTCCTCATCCAGGCCTTGTCAATGTCTTTCTCGATAACCGTGTAGAGAATCTCATTCAATGCTTTCATCCAAATACTGGATGGGAGACTAACTTGCGATCCATGATAGAGTTGCTGAACTCCATGAAGAAATTTGCTAACAAAAATGTGACTAGTAAAGTTTTTGAATACAAGAAGATATTTGGTCAACAAAAAGAGAAGCTCGAGTTCTTGATGCAAACCTTACACACACACATGCCAACATGTCTCATTTCATTGGAAATGGTTGAAAAAATGCTTCAAGCTCTTGATTTGCTAAGGTCTCTTGGAAATTCCTTGGGGCAAGCCAAATTCAAGAAAAATAACTTTGATTGTATAGATGAGTGCCTTAGCATACTAAGTTCACTTTCTGAGACTGTTTCCCTTCCTAAATTTAACATTGACATGAGAGGTGCTATCAATAGAGTTCAAGTAGAAAACTTTTGCTTGTCGAATGCTTGTCTGATTATGTGCACTGCATCAAGTTCTATGAAACTAATTGATCCAGCAAAGGTGAAGCCAGTGCAATTTTTAGTAATCGATGAAGCTGCACAGCTCAAAGAATGTGAATCAACAATTCCATTACAGCTGTCGGGTCTCCACCGTTGCATCCTAATCGGAGATGAGAGACAACTTCCTGCATTAGTAAAAAGCAAGATAGCAGACCAATGTGAATTTGGACGAAGTATGTTTGAAAGACTTGTAATGTTGGGATACGAAAGGAAAATGCTTAATGTTCAGTATAGAATGCATCCTTCCATTAGCTTATTCCCGTGCGAAGAGTTCTATGATGGAAAGCTATCTGATGCTCCCATTGTCAGGGAAGAAAGCTATAATAAGCTTTTCCTTGAAGGAGAAATGTACTCTTCCTATTCTTTTATTAACATAGCTAAGGGTAAAGAGCAATTTGGTCATGGACAAAGTCTGAAGAACATGGTTCAGGTTGCTGTTATTTCTGAGATAATTAAAAGTCTTTATGAAGTGTTCATGAAGACAAGGAAGAAAGTTAGCATAGGAATCATATCTCCATATAATGCTCAAGTTTATGAAATTCAGGAGGAAATTGAGCAGTACACTCGGGTTGCTAATTCTAAGTTCTCTGTAAATGTTCGTTCTGTTGATGGTTTTCAAGGTGGTGAGGAAGATATTATAATAATATCTACCGTGAGATCTAATGGTACTGGTGATGTTGGTTTTCTTTCAAATAGACAAAGAACAAATGTGGCTATGACTAGGGCTAGATACTGTCTTTGGATATTAGGAAATGCATGTACTTTAATCAACAGTGACTCTGTTTGGAGAAATGTAGTTATTGATGCTAAGAATAGGGATTGTTTCCACAATGCTGAAGAGGACAAAAAATTGGCCCGGGCTATTAAGAATGTTCTGTTCCAGATTCAACGACTGAAAGAATTTGAGTCACCATTCAAGAAACGGCGTTTAGGAGGGAAAATGGGAAATCATAACATATCAACTACTTTCTCTAGGCGAGTGGCACCAAATTAA

Protein sequence

>MsG0580028692.01.T01

MFVPSYNNLQSWSCLLESVEKERRMETTSNESEEDVSNASEEDVRLEDVVLSWTLEDVLNKNLYKDKVHKIPETFKSATEYKNSFVPLLFEETHTDLSSSLFAVSQAPFCEINNVQKTAWKLSIPKDQNQFIQFHHDIWLKSTTESDEVENIGNYKPVSGDLIAFTHIRPESLNDLNTHQNPYRIAYVKKVIKARNGIPDKISVLSCKCMKMDIKDDLQNNKELSSNCMKMDIEDDLCNNKELASECMKMNIEDDLWKNKELKLYAVYLMNMTTNVRICNALKSTSHMNIIKTVLGPRPISGENCQLCLSEPDSRSTFIKEDMIIRSQNLNESQEDAVSSCVSMMNCNHADIKLIWGPPGTGKTKTVACLLFSLLKLKTRTLTCAPTNTAILQVAIRLHRLVMDSLDHDTYGLGDIVLFGNGKRMKLSPHPGLVNVFLDNRVENLIQCFHPNTGWETNLRSMIELLNSMKKFANKNVTSKVFEYKKIFGQQKEKLEFLMQTLHTHMPTCLISLEMVEKMLQALDLLRSLGNSLGQAKFKKNNFDCIDECLSILSSLSETVSLPKFNIDMRGAINRVQVENFCLSNACLIMCTASSSMKLIDPAKVKPVQFLVIDEAAQLKECESTIPLQLSGLHRCILIGDERQLPALVKSKIADQCEFGRSMFERLVMLGYERKMLNVQYRMHPSISLFPCEEFYDGKLSDAPIVREESYNKLFLEGEMYSSYSFINIAKGKEQFGHGQSLKNMVQVAVISEIIKSLYEVFMKTRKKVSIGIISPYNAQVYEIQEEIEQYTRVANSKFSVNVRSVDGFQGGEEDIIIISTVRSNGTGDVGFLSNRQRTNVAMTRARYCLWILGNACTLINSDSVWRNVVIDAKNRDCFHNAEEDKKLARAIKNVLFQIQRLKEFESPFKKRRLGGKMGNHNISTTFSRRVAPN*