AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380012330.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380012330.01.T01 MTR_2g091190 90.076 131 13 0 1 131 91 221 4.81e-84 246
MsG0380012330.01.T01 MTR_4g079760 70.992 131 38 0 1 131 100 230 2.33e-66 201
MsG0380012330.01.T01 MTR_3g096568 82.895 76 13 0 37 112 236 311 9.74e-42 140
MsG0380012330.01.T01 MTR_2g015890 45.455 132 68 2 1 132 1 128 1.24e-31 110
MsG0380012330.01.T01 MTR_4g087920 43.182 132 74 1 1 132 108 238 2.81e-31 112
MsG0380012330.01.T01 MTR_2g015890 45.802 131 67 2 1 131 99 225 2.24e-30 109
MsG0380012330.01.T01 MTR_1g093750 36.364 132 73 3 2 129 191 315 3.38e-19 81.6
MsG0380012330.01.T01 MTR_1g018820 92.105 38 3 0 1 38 59 96 1.69e-17 72.4
MsG0380012330.01.T01 MTR_2g015890 45.000 80 41 1 1 80 1 77 2.08e-17 72.8
MsG0380012330.01.T01 MTR_2g015890 45.000 80 41 1 1 80 99 175 1.47e-16 72.8
MsG0380012330.01.T01 MTR_3g070970 33.636 110 63 2 1 100 105 214 1.39e-15 71.2
MsG0380012330.01.T01 MTR_5g066080 30.328 122 59 2 2 98 70 190 7.10e-12 60.8
MsG0380012330.01.T01 MTR_5g032375 32.381 105 57 3 2 98 85 183 1.42e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380012330.01.T01 AT3G25710 38.333 120 65 2 2 117 165 279 5.14e-19 81.3
MsG0380012330.01.T01 AT1G68810 36.641 131 76 3 2 129 207 333 5.86e-19 81.3
MsG0380012330.01.T01 AT3G56770 34.615 104 65 2 2 103 78 180 1.14e-13 65.5
MsG0380012330.01.T01 AT2G41130 31.818 132 82 3 2 131 100 225 4.46e-13 64.3
MsG0380012330.01.T01 AT2G40200 31.852 135 82 3 1 130 95 224 1.25e-12 63.2

Find 16 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTTCATCTTATGATAATA+AGG 0.187887 3:+15012920 MsG0380012330.01.T01:CDS
CTTATCTCATGATTGTCTTT+TGG 0.225833 3:-15012787 None:intergenic
TCACAAGAAGGTGGATTAAA+TGG 0.236239 3:+15012817 MsG0380012330.01.T01:CDS
AGTGATGCTCTAATTGAATA+AGG 0.252736 3:-15012844 None:intergenic
TGTAATAACTTCAGCTAGTA+AGG 0.369694 3:-15012710 None:intergenic
AAGGGCAGAGAATGCAACTT+TGG 0.427512 3:+15012939 MsG0380012330.01.T01:CDS
AGGGCAGAGAATGCAACTTT+GGG 0.451743 3:+15012940 MsG0380012330.01.T01:CDS
GTGATGCTCTAATTGAATAA+GGG 0.459428 3:-15012843 None:intergenic
ACTTCATCTTATGATAATAA+GGG 0.489015 3:+15012921 MsG0380012330.01.T01:CDS
CTTATATCAGACAACAAACC+TGG 0.520795 3:-15012883 None:intergenic
CTATGTTGTGAACACAAACC+AGG 0.553984 3:+15012865 MsG0380012330.01.T01:CDS
AATGCAGCACAAGCAAGTGA+AGG 0.583141 3:+15012754 MsG0380012330.01.T01:CDS
ATGCAGCACAAGCAAGTGAA+GGG 0.608119 3:+15012755 MsG0380012330.01.T01:CDS
AGATTTGAATCACAAGAAGG+TGG 0.613421 3:+15012808 MsG0380012330.01.T01:CDS
ATAAGATTTGAATCACAAGA+AGG 0.621854 3:+15012805 MsG0380012330.01.T01:CDS
GCAGAGAATGCAACTTTGGG+AGG 0.622770 3:+15012943 MsG0380012330.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTTCATCTTATGATAATAA+GGG + Chr3:15012921-15012940 MsG0380012330.01.T01:CDS 20.0%
! ATAAGATTTGAATCACAAGA+AGG + Chr3:15012805-15012824 MsG0380012330.01.T01:CDS 25.0%
! CACTTCATCTTATGATAATA+AGG + Chr3:15012920-15012939 MsG0380012330.01.T01:CDS 25.0%
AGTGATGCTCTAATTGAATA+AGG - Chr3:15012847-15012866 None:intergenic 30.0%
GTGATGCTCTAATTGAATAA+GGG - Chr3:15012846-15012865 None:intergenic 30.0%
TGTAATAACTTCAGCTAGTA+AGG - Chr3:15012713-15012732 None:intergenic 30.0%
! CTTATCTCATGATTGTCTTT+TGG - Chr3:15012790-15012809 None:intergenic 30.0%
AGATTTGAATCACAAGAAGG+TGG + Chr3:15012808-15012827 MsG0380012330.01.T01:CDS 35.0%
AGCAAAAAACTGTCGATACT+CGG - Chr3:15013025-15013044 None:intergenic 35.0%
CTTATATCAGACAACAAACC+TGG - Chr3:15012886-15012905 None:intergenic 35.0%
!! TCACAAGAAGGTGGATTAAA+TGG + Chr3:15012817-15012836 MsG0380012330.01.T01:CDS 35.0%
CTATGTTGTGAACACAAACC+AGG + Chr3:15012865-15012884 MsG0380012330.01.T01:CDS 40.0%
!!! GAGTATCGACAGTTTTTTGC+TGG + Chr3:15013024-15013043 MsG0380012330.01.T01:CDS 40.0%
AAGGGCAGAGAATGCAACTT+TGG + Chr3:15012939-15012958 MsG0380012330.01.T01:CDS 45.0%
AATGCAGCACAAGCAAGTGA+AGG + Chr3:15012754-15012773 MsG0380012330.01.T01:CDS 45.0%
AGGGCAGAGAATGCAACTTT+GGG + Chr3:15012940-15012959 MsG0380012330.01.T01:CDS 45.0%
ATGCAGCACAAGCAAGTGAA+GGG + Chr3:15012755-15012774 MsG0380012330.01.T01:CDS 45.0%
GCAGAGAATGCAACTTTGGG+AGG + Chr3:15012943-15012962 MsG0380012330.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr3 gene 15012697 15013095 15012697 ID=MsG0380012330.01;Name=MsG0380012330.01
Chr3 mRNA 15012697 15013095 15012697 ID=MsG0380012330.01.T01;Parent=MsG0380012330.01;Name=MsG0380012330.01.T01;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|0|1|0|132
Chr3 exon 15012697 15013095 15012697 ID=MsG0380012330.01.T01:exon:731;Parent=MsG0380012330.01.T01
Chr3 CDS 15012697 15013095 15012697 ID=MsG0380012330.01.T01:cds;Parent=MsG0380012330.01.T01
Gene Sequence

>MsG0380012330.01.T01

TTTGACAAAACATCCTTACTAGCTGAAGTTATTACACATTTGAAAGAGCTGAAAACAAATGCAGCACAAGCAAGTGAAGGGTTAATTATTCCAAAAGACAATCATGAGATAAGATTTGAATCACAAGAAGGTGGATTAAATGGTTTCCCTTATTCAATTAGAGCATCACTATGTTGTGAACACAAACCAGGTTTGTTGTCTGATATAAGAAAAGCACTTAATGCACTTCATCTTATGATAATAAGGGCAGAGAATGCAACTTTGGGAGGTAGAATGAAGAATATCTTTGTCATAATTAGCTGCAAAGAACAGAATTTTGAAGATGCCGAGTATCGACAGTTTTTTGCTGGATCTGTTCATCAAGCTCTTAGATCTGTACATGATAGATTTTCTAATTAA

Protein sequence

>MsG0380012330.01.T01

FDKTSLLAEVITHLKELKTNAAQASEGLIIPKDNHEIRFESQEGGLNGFPYSIRASLCCEHKPGLLSDIRKALNALHLMIIRAENATLGGRMKNIFVIISCKEQNFEDAEYRQFFAGSVHQALRSVHDRFSN*