AlfalfaGEDB | MsG0380012361.01

M. sativa cultivar ZhongmuNo.1 / MsG0380012361.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012361.01.T01	XP_013465233.1	83.969	131	21	0	1	131	91	221	2.00E-73	229

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012361.01.T01	Q9S7Y1	40.404	99	56	2	2	98	207	304	5.26E-15	73.2

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012361.01.T01	A0A072VCU0	83.969	131	21	0	1	131	91	221	9.54e-74	229

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047957.01	MsG0380012361.01	0.801025	1.093867e-48	5.170136e-46
MsG0080047959.01	MsG0380012361.01	0.800339	1.509726e-48	7.012220e-46
MsG0180000091.01	MsG0380012361.01	0.801947	7.080960e-49	3.425344e-46
MsG0180000470.01	MsG0380012361.01	0.856436	3.222379e-62	7.691067e-59
MsG0180001063.01	MsG0380012361.01	0.824180	9.282692e-54	8.161841e-51
MsG0180001884.01	MsG0380012361.01	0.807116	5.913908e-50	3.266402e-47
MsG0180001971.01	MsG0380012361.01	0.807702	4.441816e-50	2.491058e-47
MsG0180002842.01	MsG0380012361.01	0.817410	3.346309e-52	2.435143e-49
MsG0180003686.01	MsG0380012361.01	0.800205	1.607895e-48	7.442428e-46
MsG0180004275.01	MsG0380012361.01	0.816643	4.976017e-52	3.545672e-49
MsG0180004465.01	MsG0380012361.01	0.809341	1.984939e-50	1.162447e-47
MsG0180004926.01	MsG0380012361.01	0.806661	7.382332e-50	4.029583e-47
MsG0180005050.01	MsG0380012361.01	0.805516	1.285438e-49	6.814084e-47
MsG0180005185.01	MsG0380012361.01	0.826732	2.308516e-54	2.183163e-51
MsG0180005732.01	MsG0380012361.01	0.810707	1.008423e-50	6.122591e-48
MsG0180006134.01	MsG0380012361.01	0.832312	1.015656e-55	1.130820e-52
MsG0280010722.01	MsG0380012361.01	0.835282	1.837135e-56	2.236274e-53
MsG0280010807.01	MsG0380012361.01	0.819519	1.112954e-52	8.584019e-50
MsG0280011032.01	MsG0380012361.01	0.817495	3.201524e-52	2.335236e-49
MsG0380011617.01	MsG0380012361.01	0.819456	1.150507e-52	8.858299e-50
MsG0380012009.01	MsG0380012361.01	0.805182	1.510151e-49	7.937227e-47
MsG0380012070.01	MsG0380012361.01	0.800485	1.409603e-48	6.571349e-46
MsG0380012085.01	MsG0380012361.01	0.829464	5.072389e-55	5.195433e-52
MsG0380012330.01	MsG0380012361.01	0.932849	4.584373e-95	3.149018e-90
MsG0380012361.01	MsG0380012365.01	0.829533	4.881064e-55	5.009377e-52
MsG0380012361.01	MsG0380013363.01	0.801163	1.025198e-48	4.862198e-46
MsG0380012361.01	MsG0380014251.01	0.805566	1.254632e-49	6.659428e-47
MsG0380012361.01	MsG0380016210.01	0.808786	2.610088e-50	1.505952e-47
MsG0380012361.01	MsG0380016285.01	0.813430	2.569739e-51	1.677794e-48
MsG0380012361.01	MsG0380016517.01	0.807875	4.081951e-50	2.299506e-47
MsG0380012361.01	MsG0380017048.01	0.835464	1.652893e-56	2.022787e-53
MsG0380012361.01	MsG0380017543.01	0.822162	2.744966e-53	2.279422e-50
MsG0380012361.01	MsG0480018121.01	0.816290	5.967087e-52	4.211388e-49
MsG0380012361.01	MsG0480018236.01	0.821584	3.736295e-53	3.051956e-50
MsG0380012361.01	MsG0480018318.01	0.804507	2.089354e-49	1.079166e-46
MsG0380012361.01	MsG0480019190.01	0.802203	6.270983e-49	3.053406e-46
MsG0380012361.01	MsG0480019297.01	0.804188	2.434767e-49	1.247327e-46
MsG0380012361.01	MsG0480019435.01	0.821670	3.569657e-53	2.923067e-50
MsG0380012361.01	MsG0480020807.01	0.810057	1.392436e-50	8.310221e-48
MsG0380012361.01	MsG0480021276.01	0.800253	1.571642e-48	7.283753e-46
MsG0380012361.01	MsG0480021948.01	0.824266	8.859196e-54	7.808203e-51
MsG0380012361.01	MsG0480022068.01	0.817268	3.601811e-52	2.610690e-49
MsG0380012361.01	MsG0480022145.01	0.825392	4.806515e-54	4.375224e-51
MsG0380012361.01	MsG0480022169.01	0.808760	2.643910e-50	1.524441e-47
MsG0380012361.01	MsG0480022259.01	0.854671	1.053767e-61	2.371569e-58
MsG0380012361.01	MsG0480022262.01	0.863171	3.010739e-64	9.042478e-61
MsG0380012361.01	MsG0480023251.01	0.807897	4.038407e-50	2.276289e-47
MsG0380012361.01	MsG0480023654.01	0.808556	2.922464e-50	1.676142e-47
MsG0380012361.01	MsG0480023670.01	0.801769	7.702919e-49	3.709347e-46
MsG0380012361.01	MsG0580024038.01	0.809398	1.930128e-50	1.132059e-47
MsG0380012361.01	MsG0580024305.01	0.802402	5.707675e-49	2.793422e-46
MsG0380012361.01	MsG0580024306.01	0.803518	3.355879e-49	1.689913e-46
MsG0380012361.01	MsG0580024363.01	-0.809125	2.208731e-50	1.286050e-47
MsG0380012361.01	MsG0580024690.01	0.801309	9.568175e-49	4.554588e-46
MsG0380012361.01	MsG0580024781.01	0.800080	1.704793e-48	7.866072e-46
MsG0380012361.01	MsG0580025758.01	0.810198	1.298714e-50	7.778902e-48
MsG0380012361.01	MsG0580025832.01	0.802237	6.169930e-49	3.006920e-46
MsG0380012361.01	MsG0580025934.01	0.811456	6.939126e-51	4.297488e-48
MsG0380012361.01	MsG0580026122.01	0.806548	7.797533e-50	4.244276e-47
MsG0380012361.01	MsG0580026726.01	0.801524	8.645595e-49	4.137838e-46
MsG0380012361.01	MsG0580027152.01	0.802335	5.891427e-49	2.878420e-46
MsG0380012361.01	MsG0580027548.01	0.822120	2.807813e-53	2.328803e-50
MsG0380012361.01	MsG0580027947.01	0.817450	3.277785e-52	2.387912e-49
MsG0380012361.01	MsG0580028798.01	0.837393	5.338571e-57	6.924376e-54
MsG0380012361.01	MsG0680030561.01	0.812728	3.663498e-51	2.347203e-48
MsG0380012361.01	MsG0680030880.01	0.800381	1.480568e-48	6.884083e-46
MsG0380012361.01	MsG0680032157.01	0.834175	3.488132e-56	4.106394e-53
MsG0380012361.01	MsG0680032355.01	0.810198	1.298364e-50	7.776890e-48
MsG0380012361.01	MsG0680033639.01	0.806420	8.296928e-50	4.501264e-47
MsG0380012361.01	MsG0680034132.01	0.817536	3.134193e-52	2.288606e-49
MsG0380012361.01	MsG0680035444.01	0.810857	9.358848e-51	5.704998e-48
MsG0380012361.01	MsG0680035763.01	0.823680	1.215896e-53	1.054237e-50
MsG0380012361.01	MsG0780036018.01	0.804361	2.241939e-49	1.153653e-46
MsG0380012361.01	MsG0780036080.01	0.849450	3.209084e-60	6.085726e-57
MsG0380012361.01	MsG0780036201.01	0.805463	1.318711e-49	6.981433e-47
MsG0380012361.01	MsG0780037845.01	0.814421	1.554326e-51	1.042489e-48
MsG0380012361.01	MsG0780039178.01	0.801810	7.551761e-49	3.640627e-46
MsG0380012361.01	MsG0880042161.01	0.802419	5.660621e-49	2.771669e-46
MsG0380012361.01	MsG0880042247.01	0.807478	4.957132e-50	2.763922e-47
MsG0380012361.01	MsG0880043812.01	0.829495	4.986816e-55	5.112213e-52
MsG0380012361.01	MsG0880043941.01	0.800381	1.480019e-48	6.881672e-46
MsG0380012361.01	MsG0880044573.01	0.819723	1.000098e-52	7.757521e-50
MsG0380012361.01	MsG0880045117.01	0.805406	1.355420e-49	7.165168e-47
MsG0380012361.01	MsG0880045736.01	0.808615	2.839682e-50	1.631072e-47
MsG0380012361.01	MsG0880045895.01	0.824979	6.016775e-54	5.413341e-51
MsG0280006588.01	MsG0380012361.01	0.801428	9.045066e-49	4.318508e-46
MsG0280006770.01	MsG0380012361.01	0.840964	6.332372e-58	9.168164e-55
MsG0280007072.01	MsG0380012361.01	0.804196	2.426410e-49	1.243264e-46
MsG0280008524.01	MsG0380012361.01	0.806911	6.534550e-50	3.590131e-47
MsG0280009383.01	MsG0380012361.01	0.824874	6.371089e-54	5.714959e-51
MsG0280009421.01	MsG0380012361.01	0.810964	8.869892e-51	5.422815e-48
MsG0280010443.01	MsG0380012361.01	0.817698	2.881177e-52	2.113215e-49
MsG0280010517.01	MsG0380012361.01	0.802080	6.646820e-49	3.226383e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012361.01.T01	MTR_2g091190	83.969	131	21	0	1	131	91	221	2.42e-77	229
MsG0380012361.01.T01	MTR_4g079760	66.412	131	44	0	1	131	100	230	1.71e-60	187
MsG0380012361.01.T01	MTR_3g096568	75.610	82	19	1	37	118	236	316	1.12e-39	135
MsG0380012361.01.T01	MTR_4g087920	40.152	132	78	1	1	132	108	238	6.72e-28	103
MsG0380012361.01.T01	MTR_2g015890	43.182	132	71	2	1	132	1	128	2.38e-27	99.4
MsG0380012361.01.T01	MTR_2g015890	43.182	132	71	2	1	132	99	226	2.71e-26	99.4
MsG0380012361.01.T01	MTR_1g018820	92.105	38	3	0	1	38	59	96	2.86e-17	72.0
MsG0380012361.01.T01	MTR_2g015890	45.000	80	41	1	1	80	1	77	3.64e-17	72.4
MsG0380012361.01.T01	MTR_2g015890	46.154	78	39	1	1	78	99	173	2.74e-16	72.4
MsG0380012361.01.T01	MTR_1g093750	34.091	132	76	3	2	129	191	315	4.12e-16	73.2
MsG0380012361.01.T01	MTR_3g070970	31.818	110	65	2	1	100	105	214	1.06e-13	65.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012361.01.T01	AT1G68810	40.404	99	56	2	2	98	207	304	5.36e-16	73.2
MsG0380012361.01.T01	AT3G25710	34.746	118	68	2	2	115	165	277	2.46e-15	71.2
MsG0380012361.01.T01	AT3G56770	32.692	104	67	2	2	103	78	180	1.18e-11	60.1
MsG0380012361.01.T01	AT2G41130	29.545	132	85	3	2	131	100	225	5.28e-11	58.5

Find 17 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACTTCATCTTGTGATAATA+AGG	0.167508	3:+15445105	MsG0380012361.01.T01:CDS
CTTATCTCATGATTGTCTTT+TGG	0.225833	3:-15444972	None:intergenic
TCACAAGAAGGTGGATTAAA+TGG	0.236239	3:+15445002	MsG0380012361.01.T01:CDS
AGTGATGCTCTAATTGAATA+AGG	0.252736	3:-15445029	None:intergenic
TGTAATAACTTCAGCTAGTA+AGG	0.369694	3:-15444895	None:intergenic
ACTTCATCTTGTGATAATAA+GGG	0.370541	3:+15445106	MsG0380012361.01.T01:CDS
GTGATGCTCTAATTGAATAA+GGG	0.459428	3:-15445028	None:intergenic
CTTATATCAGACAACAAACC+TGG	0.520795	3:-15445068	None:intergenic
CTATGTTGTGAACACAAACC+AGG	0.553984	3:+15445050	MsG0380012361.01.T01:CDS
AAGGGGCAGAGAATGCAACT+TGG	0.561924	3:+15445124	MsG0380012361.01.T01:CDS
AATGCAGCACAAGCAAGTGA+AGG	0.583141	3:+15444939	MsG0380012361.01.T01:CDS
AGGGGCAGAGAATGCAACTT+GGG	0.594239	3:+15445125	MsG0380012361.01.T01:CDS
CTTCATCTTGTGATAATAAG+GGG	0.600825	3:+15445107	MsG0380012361.01.T01:CDS
ATGCAGCACAAGCAAGTGAA+GGG	0.608119	3:+15444940	MsG0380012361.01.T01:CDS
AGATTTGAATCACAAGAAGG+TGG	0.613421	3:+15444993	MsG0380012361.01.T01:CDS
ATAAGATTTGAATCACAAGA+AGG	0.621854	3:+15444990	MsG0380012361.01.T01:CDS
GGCAGAGAATGCAACTTGGG+AGG	0.659871	3:+15445128	MsG0380012361.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ACTTCATCTTGTGATAATAA+GGG	+	Chr3:15445106-15445125	MsG0380012361.01.T01:CDS	25.0%
!	ATAAGATTTGAATCACAAGA+AGG	+	Chr3:15444990-15445009	MsG0380012361.01.T01:CDS	25.0%
	AGTGATGCTCTAATTGAATA+AGG	-	Chr3:15445032-15445051	None:intergenic	30.0%
	CACTTCATCTTGTGATAATA+AGG	+	Chr3:15445105-15445124	MsG0380012361.01.T01:CDS	30.0%
	CTTCATCTTGTGATAATAAG+GGG	+	Chr3:15445107-15445126	MsG0380012361.01.T01:CDS	30.0%
	GTGATGCTCTAATTGAATAA+GGG	-	Chr3:15445031-15445050	None:intergenic	30.0%
	TGTAATAACTTCAGCTAGTA+AGG	-	Chr3:15444898-15444917	None:intergenic	30.0%
!	CTTATCTCATGATTGTCTTT+TGG	-	Chr3:15444975-15444994	None:intergenic	30.0%
	AGATTTGAATCACAAGAAGG+TGG	+	Chr3:15444993-15445012	MsG0380012361.01.T01:CDS	35.0%
	CTTATATCAGACAACAAACC+TGG	-	Chr3:15445071-15445090	None:intergenic	35.0%
	GCAAAAAAACTGTCGATACT+CGG	-	Chr3:15445210-15445229	None:intergenic	35.0%
!!	TCACAAGAAGGTGGATTAAA+TGG	+	Chr3:15445002-15445021	MsG0380012361.01.T01:CDS	35.0%
!!!	AGTATCGACAGTTTTTTTGC+TGG	+	Chr3:15445210-15445229	MsG0380012361.01.T01:CDS	35.0%
	CTATGTTGTGAACACAAACC+AGG	+	Chr3:15445050-15445069	MsG0380012361.01.T01:CDS	40.0%
	AATGCAGCACAAGCAAGTGA+AGG	+	Chr3:15444939-15444958	MsG0380012361.01.T01:CDS	45.0%
	ATGCAGCACAAGCAAGTGAA+GGG	+	Chr3:15444940-15444959	MsG0380012361.01.T01:CDS	45.0%
	AAGGGGCAGAGAATGCAACT+TGG	+	Chr3:15445124-15445143	MsG0380012361.01.T01:CDS	50.0%
	AGGGGCAGAGAATGCAACTT+GGG	+	Chr3:15445125-15445144	MsG0380012361.01.T01:CDS	50.0%
	GGCAGAGAATGCAACTTGGG+AGG	+	Chr3:15445128-15445147	MsG0380012361.01.T01:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	15444882	15445280	15444882	ID=MsG0380012361.01;Name=MsG0380012361.01
Chr3	mRNA	15444882	15445280	15444882	ID=MsG0380012361.01.T01;Parent=MsG0380012361.01;Name=MsG0380012361.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|132
Chr3	exon	15444882	15445280	15444882	ID=MsG0380012361.01.T01:exon:940;Parent=MsG0380012361.01.T01
Chr3	CDS	15444882	15445280	15444882	ID=MsG0380012361.01.T01:cds;Parent=MsG0380012361.01.T01

Gene Sequence

>MsG0380012361.01.T01

TTTGACAAAACATCCTTACTAGCTGAAGTTATTACACATTTGAAAGAGCTGAAAACAAATGCAGCACAAGCAAGTGAAGGGTTAATTATTCCAAAAGACAATCATGAGATAAGATTTGAATCACAAGAAGGTGGATTAAATGGTTTCCCTTATTCAATTAGAGCATCACTATGTTGTGAACACAAACCAGGTTTGTTGTCTGATATAAGAAAAGCACTTAATGCACTTCATCTTGTGATAATAAGGGGCAGAGAATGCAACTTGGGAGGTAGAATGAAGAATATCTTTGTCATAATTAGCTGCAAAGAACAGAATTTTGAAGATGCCGAGTATCGACAGTTTTTTTGCTGGATTGTTCATCAAGCTCTTAGATCTGTACATGATAGATTTTCTAATTAA

Protein sequence

>MsG0380012361.01.T01

FDKTSLLAEVITHLKELKTNAAQASEGLIIPKDNHEIRFESQEGGLNGFPYSIRASLCCEHKPGLLSDIRKALNALHLVIIRGRECNLGGRMKNIFVIISCKEQNFEDAEYRQFFCWIVHQALRSVHDRFSN*