AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380012244.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380012244.01.T01 MTR_3g019160 89.706 204 8 1 1 204 1 191 2.30e-129 362
MsG0380012244.01.T01 MTR_3g019150 79.412 204 28 2 1 204 1 190 1.77e-112 319
MsG0380012244.01.T01 MTR_3g019090 72.059 204 44 1 1 204 1 191 5.75e-104 298
MsG0380012244.01.T01 MTR_8g039090 70.297 202 47 1 3 204 4 192 1.72e-102 294
MsG0380012244.01.T01 MTR_3g019120 63.861 202 63 2 1 202 1 192 8.20e-85 256
MsG0380012244.01.T01 MTR_3g019120 63.861 202 63 2 1 202 1 192 1.49e-83 256
MsG0380012244.01.T01 MTR_3g019090 77.465 142 32 0 63 204 1 142 4.67e-79 233
MsG0380012244.01.T01 MTR_3g019120 72.973 148 40 0 55 202 5 152 4.49e-74 231
MsG0380012244.01.T01 MTR_3g019150 88.119 101 12 0 104 204 34 134 6.92e-62 189
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380012244.01.T01 AT3G27890 67.500 200 52 1 4 203 10 196 3.47e-91 265

Find 53 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCCATTTCAAGCTATTTC+TGG 0.136711 3:-13235737 None:intergenic
AACTCCCTCCAAATTATTCT+TGG 0.252574 3:-13235653 None:intergenic
TCTTGTCACATTGTTCATTC+TGG 0.267855 3:+13235937 MsG0380012244.01.T01:three_prime_UTR
TGAAGATGCCAAGAATAATT+TGG 0.284270 3:+13235645 MsG0380012244.01.T01:CDS
AAAACGAACAAATTATGATT+TGG 0.313283 3:-13235986 None:intergenic
TCAACCGGAATGGATGTAAA+TGG 0.314700 3:-13234271 None:intergenic
CATCAAGACCACCAAATGTT+TGG 0.317863 3:+13235446 MsG0380012244.01.T01:CDS
ATTTGTTCTTTATTTACATT+TGG 0.343120 3:+13235823 MsG0380012244.01.T01:three_prime_UTR
GGCTTTCTAGCCCAAACATT+TGG 0.344063 3:-13235457 None:intergenic
CACTACCATCTCCGACAAAT+TGG 0.356266 3:+13235526 MsG0380012244.01.T01:CDS
CAATCAATTGCATTCTTTAG+TGG 0.360817 3:-13235421 None:intergenic
TAACATGGCTGGAGTAATTA+AGG 0.366124 3:+13234167 None:intergenic
CAGCCTCCAGCAAAATTTAA+CGG 0.370197 3:+13235604 MsG0380012244.01.T01:CDS
ATTCTCTTCATTTGTACAAA+AGG 0.380051 3:-13236012 None:intergenic
ACATCCATTTACATCCATTC+CGG 0.388570 3:+13234267 MsG0380012244.01.T01:CDS
CACTAAAGAATGCAATTGAT+TGG 0.393700 3:+13235422 MsG0380012244.01.T01:CDS
GTTCCAGAAATAGCTTGAAA+TGG 0.396395 3:+13235734 MsG0380012244.01.T01:three_prime_UTR
ATTAAGGTCGTTGCTATCTC+TGG 0.409498 3:+13234183 MsG0380012244.01.T01:CDS
CGAAATGCTTCAACTTCAGC+TGG 0.409631 3:-13234603 None:intergenic
GCATTTACCCTTCAACTTCA+AGG 0.415648 3:+13235691 MsG0380012244.01.T01:CDS
GAGACAGAATAATTATATTC+AGG 0.417383 3:-13234666 None:intergenic
ATCAAGACCACCAAATGTTT+GGG 0.428497 3:+13235447 MsG0380012244.01.T01:CDS
GAATATAATTATTCTGTCTC+AGG 0.442769 3:+13234669 MsG0380012244.01.T01:CDS
TTGAAGGGTAAATGCCTTCA+AGG 0.460642 3:-13235683 None:intergenic
AACACCGGTCTCATCCGTTC+TGG 0.467180 3:+13234228 MsG0380012244.01.T01:CDS
ACTAAAGAATGCAATTGATT+GGG 0.467662 3:+13235423 MsG0380012244.01.T01:CDS
TGTTCATTCTGGTATTACAT+AGG 0.484792 3:+13235948 MsG0380012244.01.T01:three_prime_UTR
AAATTATGATTTGGAACGAC+CGG 0.488555 3:-13235977 None:intergenic
TTTCTAGCCCAAACATTTGG+TGG 0.496551 3:-13235454 None:intergenic
TGTAAATGGATGTAAGAAGT+AGG 0.525606 3:-13234257 None:intergenic
TGATTAACAAATAACATGGC+TGG 0.529188 3:+13234156 None:intergenic
CTGCTCTCATTCAACCGGAA+TGG 0.535184 3:-13234281 None:intergenic
CCAGCAAAATTTAACGGTGA+TGG 0.537439 3:+13235610 MsG0380012244.01.T01:CDS
CGACGAAAACTCCAATTTGT+CGG 0.538450 3:-13235537 None:intergenic
GCTGCAATAGTGAGTGTTGG+AGG 0.549316 3:+13235481 MsG0380012244.01.T01:CDS
AAACTCCAATTTGTCGGAGA+TGG 0.555469 3:-13235531 None:intergenic
AACACTGATCTTGAGACAGA+AGG 0.569151 3:+13234573 MsG0380012244.01.T01:CDS
CCGGTCTCATCCGTTCTGGT+TGG 0.571922 3:+13234232 MsG0380012244.01.T01:CDS
AGAACGGATGAGACCGGTGT+TGG 0.572988 3:-13234226 None:intergenic
CCAACACTCACTATTGCAGC+AGG 0.576426 3:-13235478 None:intergenic
TCAAGATCAGTGTTCAACAT+TGG 0.577534 3:-13234564 None:intergenic
CGAAAAGCTTCTTCCAACAC+CGG 0.586905 3:+13234213 MsG0380012244.01.T01:CDS
ACACTGATCTTGAGACAGAA+GGG 0.588342 3:+13234574 MsG0380012244.01.T01:CDS
GTGAGTGTTGGAGGAGGACA+CGG 0.590742 3:+13235490 MsG0380012244.01.T01:CDS
CCAACCAGAACGGATGAGAC+CGG 0.599652 3:-13234232 None:intergenic
AGATGCCAAGAATAATTTGG+AGG 0.599992 3:+13235648 MsG0380012244.01.T01:CDS
GATGCCAAGAATAATTTGGA+GGG 0.601338 3:+13235649 MsG0380012244.01.T01:CDS
CCTGCTGCAATAGTGAGTGT+TGG 0.618578 3:+13235478 MsG0380012244.01.T01:CDS
GAAGTAGGAACCAACCAGAA+CGG 0.644564 3:-13234242 None:intergenic
GCAATAGTGAGTGTTGGAGG+AGG 0.656596 3:+13235484 MsG0380012244.01.T01:CDS
ATAACATTGTAAATCAAACA+AGG 0.698016 3:-13235872 None:intergenic
TTGTAAATCAAACAAGGACA+AGG 0.707064 3:-13235866 None:intergenic
AGTGTTGGAGGAGGACACGG+TGG 0.780760 3:+13235493 MsG0380012244.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATATGTTGAAATTTAAAT+GGG + Chr3:13235044-13235063 MsG0380012244.01.T01:intron 10.0%
!!! TTTACTTATATATTTCATTT+TGG - Chr3:13234825-13234844 None:intergenic 10.0%
!! CTAATATGTTGAAATTTAAA+TGG + Chr3:13235043-13235062 MsG0380012244.01.T01:intron 15.0%
!! TGATAAAAAAAATCAATACT+TGG - Chr3:13235266-13235285 None:intergenic 15.0%
!!! ATTTGTTCTTTATTTACATT+TGG + Chr3:13235823-13235842 MsG0380012244.01.T01:three_prime_UTR 15.0%
!!! TAAATTTTACCAATTAAAGA+TGG + Chr3:13235113-13235132 MsG0380012244.01.T01:intron 15.0%
!! AAAACGAACAAATTATGATT+TGG - Chr3:13235989-13236008 None:intergenic 20.0%
!! ATAACATTGTAAATCAAACA+AGG - Chr3:13235875-13235894 None:intergenic 20.0%
!! TAACAACAAATTCTCTAATT+CGG - Chr3:13235177-13235196 None:intergenic 20.0%
!! TGTTTGTGTTTAATTAAAGA+TGG + Chr3:13235138-13235157 MsG0380012244.01.T01:intron 20.0%
!! TTATAAAGTGAATTAGAGAA+TGG + Chr3:13235322-13235341 MsG0380012244.01.T01:intron 20.0%
!!! ATTTTAATTATTATCGACGA+GGG + Chr3:13234473-13234492 MsG0380012244.01.T01:intron 20.0%
!!! TTCTCAACATGTTATTTTTT+TGG + Chr3:13235001-13235020 MsG0380012244.01.T01:intron 20.0%
! ACTAAAGAATGCAATTGATT+GGG + Chr3:13235423-13235442 MsG0380012244.01.T01:CDS 25.0%
! ATAATTTAGACCAAATGCAT+TGG - Chr3:13234863-13234882 None:intergenic 25.0%
! ATTCTCTTCATTTGTACAAA+AGG - Chr3:13236015-13236034 None:intergenic 25.0%
! GAATATAATTATTCTGTCTC+AGG + Chr3:13234669-13234688 MsG0380012244.01.T01:CDS 25.0%
! GAGACAGAATAATTATATTC+AGG - Chr3:13234669-13234688 None:intergenic 25.0%
!! AACAACACTTTTATTGTCAA+AGG - Chr3:13234741-13234760 None:intergenic 25.0%
!!! GATTTTAATTATTATCGACG+AGG + Chr3:13234472-13234491 MsG0380012244.01.T01:intron 25.0%
AAACGCGTTTAAGAAAAACT+CGG - Chr3:13235584-13235603 None:intergenic 30.0%
AAGAAAACATCATGTTCATG+TGG + Chr3:13234693-13234712 MsG0380012244.01.T01:intron 30.0%
ACAAACACACCATCTTTAAT+TGG - Chr3:13235125-13235144 None:intergenic 30.0%
AGAAAACATCATGTTCATGT+GGG + Chr3:13234694-13234713 MsG0380012244.01.T01:intron 30.0%
CAATCAATTGCATTCTTTAG+TGG - Chr3:13235424-13235443 None:intergenic 30.0%
CACTAAAGAATGCAATTGAT+TGG + Chr3:13235422-13235441 MsG0380012244.01.T01:CDS 30.0%
GTATAAAAGTCCAATGCATT+TGG + Chr3:13234850-13234869 MsG0380012244.01.T01:intron 30.0%
TGAAGATGCCAAGAATAATT+TGG + Chr3:13235645-13235664 MsG0380012244.01.T01:CDS 30.0%
TGGGTCATACTAAATTTATG+TGG + Chr3:13235063-13235082 MsG0380012244.01.T01:intron 30.0%
TGTAAATGGATGTAAGAAGT+AGG - Chr3:13234260-13234279 None:intergenic 30.0%
TGTTCATTCTGGTATTACAT+AGG + Chr3:13235948-13235967 MsG0380012244.01.T01:three_prime_UTR 30.0%
TGTTGAAAAGAGTTTAGTAG+AGG - Chr3:13234952-13234971 None:intergenic 30.0%
TTGTAAATCAAACAAGGACA+AGG - Chr3:13235869-13235888 None:intergenic 30.0%
! AAATTATGATTTGGAACGAC+CGG - Chr3:13235980-13235999 None:intergenic 30.0%
! AATTTTTGCCTTGAAGTTGA+AGG - Chr3:13235702-13235721 None:intergenic 30.0%
! ATTCCATTTCAAGCTATTTC+TGG - Chr3:13235740-13235759 None:intergenic 30.0%
! ATTTTTGCCTTGAAGTTGAA+GGG - Chr3:13235701-13235720 None:intergenic 30.0%
! CTTCAGTCGTGATTTTTATT+CGG + Chr3:13235788-13235807 MsG0380012244.01.T01:three_prime_UTR 30.0%
! GTGAATTAGAGAATGGTTTT+TGG + Chr3:13235329-13235348 MsG0380012244.01.T01:intron 30.0%
!! GTTTACGTGCATTGATTAAT+TGG + Chr3:13234437-13234456 MsG0380012244.01.T01:intron 30.0%
!!! GGTTTTTGGAATATTTGAGA+CGG + Chr3:13235343-13235362 MsG0380012244.01.T01:intron 30.0%
AAAACATCATGTTCATGTGG+GGG + Chr3:13234696-13234715 MsG0380012244.01.T01:intron 35.0%
AACGCGTTTAAGAAAAACTC+GGG - Chr3:13235583-13235602 None:intergenic 35.0%
AACTCCCTCCAAATTATTCT+TGG - Chr3:13235656-13235675 None:intergenic 35.0%
ACATCCATTTACATCCATTC+CGG + Chr3:13234267-13234286 MsG0380012244.01.T01:CDS 35.0%
AGATGCCAAGAATAATTTGG+AGG + Chr3:13235648-13235667 MsG0380012244.01.T01:CDS 35.0%
ATCAAGACCACCAAATGTTT+GGG + Chr3:13235447-13235466 MsG0380012244.01.T01:CDS 35.0%
GAAAACATCATGTTCATGTG+GGG + Chr3:13234695-13234714 MsG0380012244.01.T01:intron 35.0%
GATGCCAAGAATAATTTGGA+GGG + Chr3:13235649-13235668 MsG0380012244.01.T01:CDS 35.0%
GTTCCAGAAATAGCTTGAAA+TGG + Chr3:13235734-13235753 MsG0380012244.01.T01:three_prime_UTR 35.0%
TCAAGATCAGTGTTCAACAT+TGG - Chr3:13234567-13234586 None:intergenic 35.0%
TCTTGTCACATTGTTCATTC+TGG + Chr3:13235937-13235956 MsG0380012244.01.T01:three_prime_UTR 35.0%
! GGTATTACATAGGTCTTTTC+CGG + Chr3:13235958-13235977 MsG0380012244.01.T01:three_prime_UTR 35.0%
AAACTCCAATTTGTCGGAGA+TGG - Chr3:13235534-13235553 None:intergenic 40.0%
AACACTGATCTTGAGACAGA+AGG + Chr3:13234573-13234592 MsG0380012244.01.T01:CDS 40.0%
ACACTGATCTTGAGACAGAA+GGG + Chr3:13234574-13234593 MsG0380012244.01.T01:CDS 40.0%
ATTAAGGTCGTTGCTATCTC+TGG + Chr3:13234183-13234202 MsG0380012244.01.T01:CDS 40.0%
CAGCCTCCAGCAAAATTTAA+CGG + Chr3:13235604-13235623 MsG0380012244.01.T01:CDS 40.0%
CATCAAGACCACCAAATGTT+TGG + Chr3:13235446-13235465 MsG0380012244.01.T01:CDS 40.0%
CCAGCAAAATTTAACGGTGA+TGG + Chr3:13235610-13235629 MsG0380012244.01.T01:CDS 40.0%
CGACGAAAACTCCAATTTGT+CGG - Chr3:13235540-13235559 None:intergenic 40.0%
GATTGAGCTGAGCAAAAATG+TGG + Chr3:13234500-13234519 MsG0380012244.01.T01:CDS 40.0%
GCATTTACCCTTCAACTTCA+AGG + Chr3:13235691-13235710 MsG0380012244.01.T01:CDS 40.0%
TCAACCGGAATGGATGTAAA+TGG - Chr3:13234274-13234293 None:intergenic 40.0%
TTGAAGGGTAAATGCCTTCA+AGG - Chr3:13235686-13235705 None:intergenic 40.0%
! CCATCACCGTTAAATTTTGC+TGG - Chr3:13235613-13235632 None:intergenic 40.0%
! TCACCGTTAAATTTTGCTGG+AGG - Chr3:13235610-13235629 None:intergenic 40.0%
! TTTCTAGCCCAAACATTTGG+TGG - Chr3:13235457-13235476 None:intergenic 40.0%
!! GTGTTGTTTGATGTGTGTCT+TGG + Chr3:13234753-13234772 MsG0380012244.01.T01:intron 40.0%
CACTACCATCTCCGACAAAT+TGG + Chr3:13235526-13235545 MsG0380012244.01.T01:CDS 45.0%
CGAAAAGCTTCTTCCAACAC+CGG + Chr3:13234213-13234232 MsG0380012244.01.T01:CDS 45.0%
CGAAATGCTTCAACTTCAGC+TGG - Chr3:13234606-13234625 None:intergenic 45.0%
CTGAGCAAAAATGTGGTCGA+GGG + Chr3:13234507-13234526 MsG0380012244.01.T01:CDS 45.0%
GAAGTAGGAACCAACCAGAA+CGG - Chr3:13234245-13234264 None:intergenic 45.0%
TAACGACGAAATTGACGTGC+TGG + Chr3:13234349-13234368 MsG0380012244.01.T01:intron 45.0%
! GGCTTTCTAGCCCAAACATT+TGG - Chr3:13235460-13235479 None:intergenic 45.0%
! GGGAGTTCTTTTGTCCTTGA+AGG + Chr3:13235669-13235688 MsG0380012244.01.T01:CDS 45.0%
CCAACACTCACTATTGCAGC+AGG - Chr3:13235481-13235500 None:intergenic 50.0%
CCTGCTGCAATAGTGAGTGT+TGG + Chr3:13235478-13235497 MsG0380012244.01.T01:CDS 50.0%
CTGCTCTCATTCAACCGGAA+TGG - Chr3:13234284-13234303 None:intergenic 50.0%
GCACACTGCTCTCATTCAAC+CGG - Chr3:13234289-13234308 None:intergenic 50.0%
GCTGAGCAAAAATGTGGTCG+AGG + Chr3:13234506-13234525 MsG0380012244.01.T01:CDS 50.0%
!! GCAATAGTGAGTGTTGGAGG+AGG + Chr3:13235484-13235503 MsG0380012244.01.T01:CDS 50.0%
!! GCTGCAATAGTGAGTGTTGG+AGG + Chr3:13235481-13235500 MsG0380012244.01.T01:CDS 50.0%
AACACCGGTCTCATCCGTTC+TGG + Chr3:13234228-13234247 MsG0380012244.01.T01:CDS 55.0%
AGAACGGATGAGACCGGTGT+TGG - Chr3:13234229-13234248 None:intergenic 55.0%
CCAACCAGAACGGATGAGAC+CGG - Chr3:13234235-13234254 None:intergenic 55.0%
!! GTGAGTGTTGGAGGAGGACA+CGG + Chr3:13235490-13235509 MsG0380012244.01.T01:CDS 55.0%
CCGGTCTCATCCGTTCTGGT+TGG + Chr3:13234232-13234251 MsG0380012244.01.T01:CDS 60.0%
! AGTGTTGGAGGAGGACACGG+TGG + Chr3:13235493-13235512 MsG0380012244.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr3 gene 13234171 13236044 13234171 ID=MsG0380012244.01;Name=MsG0380012244.01
Chr3 mRNA 13234171 13236044 13234171 ID=MsG0380012244.01.T01;Parent=MsG0380012244.01;Name=MsG0380012244.01.T01;_AED=0.38;_eAED=0.41;_QI=0|0.5|0.66|1|1|1|3|321|204
Chr3 exon 13234171 13234288 13234171 ID=MsG0380012244.01.T01:exon:468;Parent=MsG0380012244.01.T01
Chr3 exon 13234499 13234690 13234499 ID=MsG0380012244.01.T01:exon:469;Parent=MsG0380012244.01.T01
Chr3 exon 13235419 13236044 13235419 ID=MsG0380012244.01.T01:exon:470;Parent=MsG0380012244.01.T01
Chr3 CDS 13234171 13234288 13234171 ID=MsG0380012244.01.T01:cds;Parent=MsG0380012244.01.T01
Chr3 CDS 13234499 13234690 13234499 ID=MsG0380012244.01.T01:cds;Parent=MsG0380012244.01.T01
Chr3 CDS 13235419 13235723 13235419 ID=MsG0380012244.01.T01:cds;Parent=MsG0380012244.01.T01
Chr3 three_prime_UTR 13235724 13236044 13235724 ID=MsG0380012244.01.T01:three_prime_utr;Parent=MsG0380012244.01.T01
Gene Sequence

>MsG0380012244.01.T01

ATGGCTGGAGTAATTAAGGTCGTTGCTATCTCTGGTTCTCTTCGAAAAGCTTCTTCCAACACCGGTCTCATCCGTTCTGGTTGGTTCCTACTTCTTACATCCATTTACATCCATTCCGCGATTGAGCTGAGCAAAAATGTGGTCGAGGGTGTTGATATTGAGTACATTGATATTTCAAATCTTCCAATGTTGAACACTGATCTTGAGACAGAAGGGAATTTTCCAGCTGAAGTTGAAGCATTTCGTCAAAAGATTCTTGCAGCTGATAGTGTTCTCTTTGCTTCTCCTGAATATAATTATTCTGTCTCAGCTCCACTAAAGAATGCAATTGATTGGGCATCAAGACCACCAAATGTTTGGGCTAGAAAGCCTGCTGCAATAGTGAGTGTTGGAGGAGGACACGGTGGTGCAAAGTCACACTACCATCTCCGACAAATTGGAGTTTTCGTCGATCTTCATTTCATCAATAAACCCGAGTTTTTCTTAAACGCGTTTCAGCCTCCAGCAAAATTTAACGGTGATGGTGATTTGATCGATGAAGATGCCAAGAATAATTTGGAGGGAGTTCTTTTGTCCTTGAAGGCATTTACCCTTCAACTTCAAGGCAAAAATTGA

Protein sequence

>MsG0380012244.01.T01

MAGVIKVVAISGSLRKASSNTGLIRSGWFLLLTSIYIHSAIELSKNVVEGVDIEYIDISNLPMLNTDLETEGNFPAEVEAFRQKILAADSVLFASPEYNYSVSAPLKNAIDWASRPPNVWARKPAAIVSVGGGHGGAKSHYHLRQIGVFVDLHFINKPEFFLNAFQPPAKFNGDGDLIDEDAKNNLEGVLLSLKAFTLQLQGKN*