AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045677.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045677.01.T01 MTR_8g076680 97.531 81 1 1 50 129 158 238 7.54e-52 164
MsG0880045677.01.T01 MTR_8g076680 97.531 81 1 1 50 129 158 238 3.02e-51 165
MsG0880045677.01.T01 MTR_7g113050 39.560 91 53 2 41 129 148 238 1.44e-11 59.7
MsG0880045677.01.T01 MTR_7g108825 34.444 90 58 1 41 129 147 236 2.03e-11 59.3
MsG0880045677.01.T01 MTR_7g113050 39.560 91 53 2 41 129 148 238 2.55e-11 59.7
MsG0880045677.01.T01 MTR_7g108825 34.444 90 58 1 41 129 147 236 3.34e-11 59.3
MsG0880045677.01.T01 MTR_4g120240 34.694 98 63 1 33 129 177 274 3.61e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045677.01.T01 AT3G51000 62.963 81 29 1 50 129 158 238 8.21e-31 112
MsG0880045677.01.T01 AT4G02340 37.634 93 50 4 41 129 147 235 6.70e-11 58.5

Find 32 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAACAGCATATAACAATT+TGG 0.237381 8:-62625591 None:intergenic
TCCAAGAAATATGGTATTCT+TGG 0.311721 8:+62625544 MsG0880045677.01.T01:CDS
GCTCATGGTTCCAAGAAATA+TGG 0.342189 8:+62625535 None:intergenic
GCCAAGAATACCATATTTCT+TGG 0.346010 8:-62625545 None:intergenic
GTGTATGAATTGGGGTTGAT+AGG 0.358412 8:-62625647 None:intergenic
GGCCAAGTCGTTTGAGTTGG+TGG 0.382321 8:-62625569 None:intergenic
TGATTTCTTGCCAACTCCTT+CGG 0.394280 8:+62627027 MsG0880045677.01.T01:CDS
GCAGACAAATTCCAAGAGTC+TGG 0.396715 8:+62627088 MsG0880045677.01.T01:CDS
TGTTGAAGAGTGTATGAATT+GGG 0.407460 8:-62625656 None:intergenic
GCAACAGCATATAACAATTT+GGG 0.414578 8:-62625590 None:intergenic
GCACCTGATCTAAGAGGATA+TGG 0.428528 8:+62625611 MsG0880045677.01.T01:CDS
CACCGGTGAAACCAGACTCT+TGG 0.472500 8:-62627099 None:intergenic
TTCCAAGAGTCTGGTTTCAC+CGG 0.475025 8:+62627097 MsG0880045677.01.T01:CDS
CGCCACCAACTCAAACGACT+TGG 0.494042 8:+62625567 MsG0880045677.01.T01:CDS
TCACCATATCCTCTTAGATC+AGG 0.494603 8:-62625614 None:intergenic
ATTGGGGTTGATAGGAGAAT+CGG 0.507484 8:-62625639 None:intergenic
ATGTTGAAGAGTGTATGAAT+TGG 0.512303 8:-62625657 None:intergenic
TTGGGCCAAGTCGTTTGAGT+TGG 0.524319 8:-62625572 None:intergenic
ATACACTCTTCAACATATGT+TGG 0.526041 8:+62625664 MsG0880045677.01.T01:CDS
TATAACAGCTCCTCCTGGCA+TGG 0.539454 8:+62626997 MsG0880045677.01.T01:CDS
TTGCGTGGTTGGCAGGAACC+AGG 0.547693 8:+62626896 MsG0880045677.01.T01:intron
AAATCAACAATCTCCATGCC+AGG 0.548033 8:-62627010 None:intergenic
GCTGTTGCACCTGATCTAAG+AGG 0.580412 8:+62625605 MsG0880045677.01.T01:CDS
AAAGCTCTCTCTGCACGACC+TGG 0.595328 8:-62626914 None:intergenic
ATCCACGACGGCAAAACCGA+AGG 0.595962 8:-62627043 None:intergenic
GACGGCAAAACCGAAGGAGT+TGG 0.599928 8:-62627037 None:intergenic
GTGGATAACCGAGAAACTCA+TGG 0.623008 8:+62627060 MsG0880045677.01.T01:CDS
GTTGAAGAGTGTATGAATTG+GGG 0.627329 8:-62625655 None:intergenic
GCGATAGTAATTGAATGCAC+CGG 0.666695 8:-62627116 None:intergenic
AGTTTCTCGGTTATCCACGA+CGG 0.704697 8:-62627055 None:intergenic
ATTCAATTACTATCGCGCAA+TGG 0.705978 8:+62627123 MsG0880045677.01.T01:CDS
TCAACAATCTCCATGCCAGG+AGG 0.721559 8:-62627007 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATTAAACAAAAAAACG+TGG - Chr8:62625760-62625779 None:intergenic 15.0%
!! TATTTCATAAATAATCTACA+CGG - Chr8:62626546-62626565 None:intergenic 15.0%
!! AAATTAGGAAATTAGGAAAA+AGG - Chr8:62626598-62626617 None:intergenic 20.0%
!! AGAGAATAAATTAGGAAATT+AGG - Chr8:62626605-62626624 None:intergenic 20.0%
!!! AACTTTAGGTTTTTACAAAT+GGG + Chr8:62626352-62626371 MsG0880045677.01.T01:intron 20.0%
!!! GTTTACATATATCGTTTTTT+AGG + Chr8:62626490-62626509 MsG0880045677.01.T01:intron 20.0%
!!! TACTTATCTATTTTCTTTAG+AGG - Chr8:62626131-62626150 None:intergenic 20.0%
!!! TGAAACTATAAGAAAGTATA+CGG + Chr8:62626773-62626792 MsG0880045677.01.T01:intron 20.0%
!!! TTATATCAAGTTGAATATTG+TGG - Chr8:62625933-62625952 None:intergenic 20.0%
! AAACGATATATGTAAACTTG+AGG - Chr8:62626487-62626506 None:intergenic 25.0%
! AACTTTATCAAGCCATAAAA+GGG - Chr8:62626835-62626854 None:intergenic 25.0%
! AATTACAATTTCCATTGTAC+CGG + Chr8:62626245-62626264 MsG0880045677.01.T01:intron 25.0%
! GAAATATAATTGTACGCAAT+TGG + Chr8:62626560-62626579 MsG0880045677.01.T01:intron 25.0%
! GTAACTTTATGATTATTGCA+AGG + Chr8:62626431-62626450 MsG0880045677.01.T01:intron 25.0%
! GTACAATGGAAATTGTAATT+TGG - Chr8:62626245-62626264 None:intergenic 25.0%
! TAACTTTATCAAGCCATAAA+AGG - Chr8:62626836-62626855 None:intergenic 25.0%
! TATCTTTGAACGTGATTATT+AGG - Chr8:62625840-62625859 None:intergenic 25.0%
! TATGTTAATTAGTGAGTAGA+TGG + Chr8:62625858-62625877 MsG0880045677.01.T01:intron 25.0%
! TATTAAACAAAAAAACGTGG+AGG - Chr8:62625757-62625776 None:intergenic 25.0%
!! ATGTAAATCGTTTGTGTTTT+GGG - Chr8:62626160-62626179 None:intergenic 25.0%
!! TAATTTTCATTGAAATTGCG+TGG + Chr8:62626881-62626900 MsG0880045677.01.T01:intron 25.0%
!! TATGTAAATCGTTTGTGTTT+TGG - Chr8:62626161-62626180 None:intergenic 25.0%
!!! ACTTTAGGTTTTTACAAATG+GGG + Chr8:62626353-62626372 MsG0880045677.01.T01:intron 25.0%
!!! CAACTTTAGGTTTTTACAAA+TGG + Chr8:62626351-62626370 MsG0880045677.01.T01:intron 25.0%
!!! TCGTTTTTTAGGTGTAATTT+AGG + Chr8:62626501-62626520 MsG0880045677.01.T01:intron 25.0%
!!! TTATCTATTTTCTTTAGAGG+TGG - Chr8:62626128-62626147 None:intergenic 25.0%
AATGATCAAGAAGACCAATA+AGG - Chr8:62625693-62625712 None:intergenic 30.0%
ACAATTGCATGATGAGATTA+AGG + Chr8:62625980-62625999 MsG0880045677.01.T01:intron 30.0%
ACATATGCCTTCATTCAAAT+GGG - Chr8:62626632-62626651 None:intergenic 30.0%
ATACACTCTTCAACATATGT+TGG + Chr8:62625664-62625683 MsG0880045677.01.T01:CDS 30.0%
ATTCACTCATAACTCACAAT+GGG - Chr8:62626319-62626338 None:intergenic 30.0%
GCAACAGCATATAACAATTT+GGG - Chr8:62625593-62625612 None:intergenic 30.0%
GTTACTAATGCATCATGAAA+TGG - Chr8:62626416-62626435 None:intergenic 30.0%
TAATCTCATCATGCAATTGT+GGG - Chr8:62625980-62625999 None:intergenic 30.0%
TACATATGCCTTCATTCAAA+TGG - Chr8:62626633-62626652 None:intergenic 30.0%
TAGTTTCAACAGTTTATCTG+TGG - Chr8:62626761-62626780 None:intergenic 30.0%
TATATGTAAACTTGAGGTCT+TGG - Chr8:62626481-62626500 None:intergenic 30.0%
TCCAAGAAATATGGTATTCT+TGG + Chr8:62625544-62625563 MsG0880045677.01.T01:CDS 30.0%
TGCAACAGCATATAACAATT+TGG - Chr8:62625594-62625613 None:intergenic 30.0%
TGGGATAGAGAGAATAAATT+AGG - Chr8:62626613-62626632 None:intergenic 30.0%
TGGTCATATTCAACAACTTT+AGG + Chr8:62626338-62626357 MsG0880045677.01.T01:intron 30.0%
TTAATCTCATCATGCAATTG+TGG - Chr8:62625981-62626000 None:intergenic 30.0%
TTAGGAAAAAGGATAGTTTG+AGG - Chr8:62626587-62626606 None:intergenic 30.0%
TTGTGGTCAATTCAACTATA+AGG + Chr8:62625904-62625923 MsG0880045677.01.T01:intron 30.0%
! AATTTTGCCTACAACATGAA+AGG - Chr8:62626381-62626400 None:intergenic 30.0%
! ATGTTGAAGAGTGTATGAAT+TGG - Chr8:62625660-62625679 None:intergenic 30.0%
! TATAAGAAAGTATACGGAGA+TGG + Chr8:62626779-62626798 MsG0880045677.01.T01:intron 30.0%
! TGTTGAAGAGTGTATGAATT+GGG - Chr8:62625659-62625678 None:intergenic 30.0%
! TTGATCATTTGGTGAACAAA+AGG + Chr8:62625704-62625723 MsG0880045677.01.T01:intron 30.0%
!! AGAAGTTTTTGCTGATTACA+TGG + Chr8:62626966-62626985 MsG0880045677.01.T01:CDS 30.0%
!! GTGTTTTTGACTCAAACATA+TGG - Chr8:62626459-62626478 None:intergenic 30.0%
!! TATTGGTCTTCTTGATCATT+TGG + Chr8:62625693-62625712 MsG0880045677.01.T01:intron 30.0%
AAGGGTAATCTACCTGAAAT+TGG - Chr8:62626817-62626836 None:intergenic 35.0%
ATATGTAGTAGGATCTGACT+GGG + Chr8:62626646-62626665 MsG0880045677.01.T01:intron 35.0%
ATTCAATTACTATCGCGCAA+TGG + Chr8:62627123-62627142 MsG0880045677.01.T01:CDS 35.0%
CATTCACTCATAACTCACAA+TGG - Chr8:62626320-62626339 None:intergenic 35.0%
CATTGTGAGTTATGAGTGAA+TGG + Chr8:62626318-62626337 MsG0880045677.01.T01:intron 35.0%
GCCAAGAATACCATATTTCT+TGG - Chr8:62625548-62625567 None:intergenic 35.0%
TACATAGAGTTCGTCAATCA+TGG + Chr8:62626175-62626194 MsG0880045677.01.T01:intron 35.0%
TATCAGGCCTAAATAGACTT+AGG - Chr8:62626691-62626710 None:intergenic 35.0%
TCATAACTCACAATGGGTAT+GGG - Chr8:62626313-62626332 None:intergenic 35.0%
TCTCTATCCCATTTGAATGA+AGG + Chr8:62626622-62626641 MsG0880045677.01.T01:intron 35.0%
TGCAAAAACCATGAGTTTCT+CGG - Chr8:62627071-62627090 None:intergenic 35.0%
TTTCATTGAAATTGCGTGGT+TGG + Chr8:62626885-62626904 MsG0880045677.01.T01:intron 35.0%
! GTTGAAGAGTGTATGAATTG+GGG - Chr8:62625658-62625677 None:intergenic 35.0%
! TCAGGTAGATTACCCTTTTA+TGG + Chr8:62626820-62626839 MsG0880045677.01.T01:intron 35.0%
! TGAATGAAGGCATATGTAGT+AGG + Chr8:62626635-62626654 MsG0880045677.01.T01:intron 35.0%
!! ACATATGTTGGTGACCTTAT+TGG + Chr8:62625676-62625695 MsG0880045677.01.T01:intron 35.0%
!!! AAATCGTTTGTGTTTTGGGA+TGG - Chr8:62626156-62626175 None:intergenic 35.0%
!!! ATTGACCACAATTTTGACTG+TGG - Chr8:62625895-62625914 None:intergenic 35.0%
AAATCAACAATCTCCATGCC+AGG - Chr8:62627013-62627032 None:intergenic 40.0%
AGCTCATGTTTGCCAATTTC+AGG + Chr8:62626802-62626821 MsG0880045677.01.T01:intron 40.0%
ATATATCTGAGCGAGTCTCA+AGG + Chr8:62626074-62626093 MsG0880045677.01.T01:intron 40.0%
ATTTAGGCCTGATAGAGTGA+AGG + Chr8:62626697-62626716 MsG0880045677.01.T01:intron 40.0%
CAACAAACTTACCGGTACAA+TGG - Chr8:62626259-62626278 None:intergenic 40.0%
CAAGTCCACAGTCAAAATTG+TGG + Chr8:62625887-62625906 MsG0880045677.01.T01:intron 40.0%
CAGTTTATCTGTGGAGATCT+TGG - Chr8:62626752-62626771 None:intergenic 40.0%
CATATGTAGTAGGATCTGAC+TGG + Chr8:62626645-62626664 MsG0880045677.01.T01:intron 40.0%
CGAATTTACTCCACCATCTA+CGG - Chr8:62626011-62626030 None:intergenic 40.0%
CTCATAACTCACAATGGGTA+TGG - Chr8:62626314-62626333 None:intergenic 40.0%
GATGGCACCTAAGTCTATTT+AGG + Chr8:62626681-62626700 MsG0880045677.01.T01:intron 40.0%
GATTAAGGATATGCCGTAGA+TGG + Chr8:62625995-62626014 MsG0880045677.01.T01:intron 40.0%
GCGATAGTAATTGAATGCAC+CGG - Chr8:62627119-62627138 None:intergenic 40.0%
TATATCTGAGCGAGTCTCAA+GGG + Chr8:62626075-62626094 MsG0880045677.01.T01:intron 40.0%
TATGTAGTAGGATCTGACTG+GGG + Chr8:62626647-62626666 MsG0880045677.01.T01:intron 40.0%
TCACCATATCCTCTTAGATC+AGG - Chr8:62625617-62625636 None:intergenic 40.0%
TGATTTCTTGCCAACTCCTT+CGG + Chr8:62627027-62627046 MsG0880045677.01.T01:CDS 40.0%
TGTGGAGATCTTGGAAAGTA+AGG - Chr8:62626743-62626762 None:intergenic 40.0%
TTTAGGCCTGATAGAGTGAA+GGG + Chr8:62626698-62626717 MsG0880045677.01.T01:intron 40.0%
! CATTTTATAACAGCTCCTCC+TGG + Chr8:62626992-62627011 MsG0880045677.01.T01:CDS 40.0%
! GTGTATGAATTGGGGTTGAT+AGG - Chr8:62625650-62625669 None:intergenic 40.0%
! TGAAAGCGCAACAAACTTAC+CGG - Chr8:62626267-62626286 None:intergenic 40.0%
!! ATTGGGGTTGATAGGAGAAT+CGG - Chr8:62625642-62625661 None:intergenic 40.0%
!! CGTAGATGGTGGAGTAAATT+CGG + Chr8:62626009-62626028 MsG0880045677.01.T01:intron 40.0%
AACGTGGAGGCTCAAGAAAA+TGG - Chr8:62625744-62625763 None:intergenic 45.0%
ACAAACCCCTTCACTCTATC+AGG - Chr8:62626707-62626726 None:intergenic 45.0%
AGTTTCTCGGTTATCCACGA+CGG - Chr8:62627058-62627077 None:intergenic 45.0%
ATTGAAATTGCGTGGTTGGC+AGG + Chr8:62626889-62626908 MsG0880045677.01.T01:intron 45.0%
GCACCTGATCTAAGAGGATA+TGG + Chr8:62625611-62625630 MsG0880045677.01.T01:CDS 45.0%
GCAGACAAATTCCAAGAGTC+TGG + Chr8:62627088-62627107 MsG0880045677.01.T01:CDS 45.0%
GTGGATAACCGAGAAACTCA+TGG + Chr8:62627060-62627079 MsG0880045677.01.T01:CDS 45.0%
TAAGGATATGCCGTAGATGG+TGG + Chr8:62625998-62626017 MsG0880045677.01.T01:intron 45.0%
TGGGGAACCTTTCATGTTGT+AGG + Chr8:62626371-62626390 MsG0880045677.01.T01:intron 45.0%
TTAGGCCTGATAGAGTGAAG+GGG + Chr8:62626699-62626718 MsG0880045677.01.T01:intron 45.0%
! TTCCAAGAGTCTGGTTTCAC+CGG + Chr8:62627097-62627116 MsG0880045677.01.T01:CDS 45.0%
ACTGGGGAGCAAACATTGGA+TGG + Chr8:62626663-62626682 MsG0880045677.01.T01:intron 50.0%
GCTGTTGCACCTGATCTAAG+AGG + Chr8:62625605-62625624 MsG0880045677.01.T01:CDS 50.0%
TATAACAGCTCCTCCTGGCA+TGG + Chr8:62626997-62627016 MsG0880045677.01.T01:CDS 50.0%
TCAACAATCTCCATGCCAGG+AGG - Chr8:62627010-62627029 None:intergenic 50.0%
TCTGACTGGGGAGCAAACAT+TGG + Chr8:62626659-62626678 MsG0880045677.01.T01:intron 50.0%
TTGGGCCAAGTCGTTTGAGT+TGG - Chr8:62625575-62625594 None:intergenic 50.0%
AAAGCTCTCTCTGCACGACC+TGG - Chr8:62626917-62626936 None:intergenic 55.0%
ATCCACGACGGCAAAACCGA+AGG - Chr8:62627046-62627065 None:intergenic 55.0%
CACCGGTGAAACCAGACTCT+TGG - Chr8:62627102-62627121 None:intergenic 55.0%
CGCCACCAACTCAAACGACT+TGG + Chr8:62625567-62625586 MsG0880045677.01.T01:CDS 55.0%
GACGGCAAAACCGAAGGAGT+TGG - Chr8:62627040-62627059 None:intergenic 55.0%
!! GGCCAAGTCGTTTGAGTTGG+TGG - Chr8:62625572-62625591 None:intergenic 55.0%
TTGCGTGGTTGGCAGGAACC+AGG + Chr8:62626896-62626915 MsG0880045677.01.T01:intron 60.0%
! CTCCTTCGGTTTTGCCGTCG+TGG + Chr8:62627041-62627060 MsG0880045677.01.T01:CDS 60.0%
GGGCGCTCGAGCATATCGAG+TGG - Chr8:62626293-62626312 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 62625539 62627153 62625539 ID=MsG0880045677.01;Name=MsG0880045677.01
Chr8 mRNA 62625539 62627153 62625539 ID=MsG0880045677.01.T01;Parent=MsG0880045677.01;Name=MsG0880045677.01.T01;_AED=0.34;_eAED=0.38;_QI=0|0|0|1|1|1|2|0|129
Chr8 exon 62625539 62625685 62625539 ID=MsG0880045677.01.T01:exon:12992;Parent=MsG0880045677.01.T01
Chr8 exon 62626911 62627153 62626911 ID=MsG0880045677.01.T01:exon:12993;Parent=MsG0880045677.01.T01
Chr8 CDS 62625539 62625685 62625539 ID=MsG0880045677.01.T01:cds;Parent=MsG0880045677.01.T01
Chr8 CDS 62626911 62627153 62626911 ID=MsG0880045677.01.T01:cds;Parent=MsG0880045677.01.T01
Gene Sequence

>MsG0880045677.01.T01

ATGGTTCCAAGAAATATGGTATTCTTGGCGCCACCAACTCAAACGACTTGGCCCAAATTGTTATATGCTGTTGCACCTGATCTAAGAGGATATGGTGACTCCGATTCTCCTATCAACCCCAATTCATACACTCTTCAACATATGTTGGAACCAGGTCGTGCAGAGAGAGCTTTTGCTAGATATGATTGTTTGACAGTGATGAAGAAGTTTTTGCTGATTACATGGACACATTTTATAACAGCTCCTCCTGGCATGGAGATTGTTGATTTCTTGCCAACTCCTTCGGTTTTGCCGTCGTGGATAACCGAGAAACTCATGGTTTTTGCAGACAAATTCCAAGAGTCTGGTTTCACCGGTGCATTCAATTACTATCGCGCAATGGACCTGTAA

Protein sequence

>MsG0880045677.01.T01

MVPRNMVFLAPPTQTTWPKLLYAVAPDLRGYGDSDSPINPNSYTLQHMLEPGRAERAFARYDCLTVMKKFLLITWTHFITAPPGMEIVDFLPTPSVLPSWITEKLMVFADKFQESGFTGAFNYYRAMDL*