AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580025870.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025870.01.T01 MTR_5g036080 95.031 322 16 0 1 322 1 322 0.0 627
MsG0580025870.01.T01 MTR_5g036410 41.311 305 146 10 41 321 33 328 1.81e-62 202
MsG0580025870.01.T01 MTR_8g062770 39.755 327 158 10 25 321 11 328 1.19e-58 193
MsG0580025870.01.T01 MTR_5g012680 35.374 294 172 8 40 321 28 315 3.15e-50 171
MsG0580025870.01.T01 MTR_5g036087 39.464 261 134 9 74 318 60 312 5.51e-49 167
MsG0580025870.01.T01 MTR_8g005900 35.688 269 150 6 71 321 46 309 4.48e-47 162
MsG0580025870.01.T01 MTR_5g036093 34.848 264 143 8 78 322 35 288 1.68e-40 144
MsG0580025870.01.T01 MTR_5g036360 32.951 349 180 17 1 322 2 323 1.78e-39 142
MsG0580025870.01.T01 MTR_5g036290 35.798 257 143 9 76 322 89 333 1.92e-39 142
MsG0580025870.01.T01 MTR_5g036270 33.115 305 141 9 74 318 3 304 3.84e-38 138
MsG0580025870.01.T01 MTR_5g036083 36.154 260 131 9 74 322 42 277 4.29e-38 138
MsG0580025870.01.T01 MTR_6g008480 29.693 293 158 10 66 318 45 329 4.53e-31 119
MsG0580025870.01.T01 MTR_5g036310 31.538 260 115 10 71 318 68 276 9.82e-28 110
MsG0580025870.01.T01 MTR_5g036110 30.556 252 108 9 74 320 54 243 1.67e-16 79.7
MsG0580025870.01.T01 MTR_5g036365 32.500 120 71 3 166 275 43 162 3.94e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025870.01.T01 AT1G70170 43.772 281 121 10 71 321 53 326 1.54e-60 198
MsG0580025870.01.T01 AT1G59970 40.809 272 133 8 67 318 50 313 6.39e-60 196
MsG0580025870.01.T01 AT1G24140 37.374 297 146 10 57 321 43 331 4.49e-55 184
MsG0580025870.01.T01 AT4G16640 36.237 287 148 12 55 321 50 321 1.25e-45 159
MsG0580025870.01.T01 AT2G45040 34.496 258 147 8 77 321 50 298 5.10e-37 135

Find 53 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAGGATTCAGGAAATTTA+TGG 0.186644 5:+25630468 MsG0580025870.01.T01:CDS
TTCCTCCACCACGGTTTAAA+TGG 0.210107 5:-25630015 None:intergenic
TGATGATTTGCAAAGGATTC+AGG 0.269973 5:+25630458 MsG0580025870.01.T01:CDS
CATCAAGTTGGTCATTTACT+AGG 0.286156 5:+25630357 MsG0580025870.01.T01:CDS
AGTTATGCGTTTAAGATAGA+AGG 0.301172 5:-25629436 None:intergenic
GTTGGTGAAGTTGTTCTTCA+TGG 0.302002 5:-25629983 None:intergenic
AATGCAAAGTTGAAGAGTTT+AGG 0.330631 5:-25629581 None:intergenic
TGAAGAGGATGATGTGGATT+TGG 0.356149 5:+25630320 MsG0580025870.01.T01:CDS
AAAGAATATTTACAATTCTT+CGG 0.396929 5:+25629555 MsG0580025870.01.T01:CDS
CTAACTATTAATCCAATTGC+TGG 0.408047 5:-25629402 None:intergenic
CTTGAGTTTATTCCAGATGC+TGG 0.411033 5:-25629475 None:intergenic
TTCAACAAAGCTCTAGAGAT+TGG 0.411452 5:+25629504 MsG0580025870.01.T01:CDS
AATTGCTGGTGCAAAAGAAA+TGG 0.419697 5:-25629388 None:intergenic
TTGTAAATATTCTTTCAAAT+CGG 0.422468 5:-25629547 None:intergenic
GTTGGTCATTTACTAGGGTT+GGG 0.423122 5:+25630363 MsG0580025870.01.T01:CDS
TTATGTTGGCAGTGTATACT+TGG 0.428399 5:+25630257 MsG0580025870.01.T01:CDS
AGTTGGTCATTTACTAGGGT+TGG 0.441522 5:+25630362 MsG0580025870.01.T01:CDS
GTGGTTCCATTGATTATGTC+GGG 0.454466 5:-25629958 None:intergenic
GATGGAAAAGGTGGAACGGT+TGG 0.463287 5:+25630210 MsG0580025870.01.T01:CDS
TCGTTTGTGACATTATTCTC+TGG 0.479157 5:-25630072 None:intergenic
GTTGGGACATTCTTCTGTTG+AGG 0.487831 5:+25630380 MsG0580025870.01.T01:CDS
TAATACTAAGAGAAACATAT+AGG 0.496205 5:-25629337 None:intergenic
GGAAAAGGTGGAACGGTTGG+AGG 0.500795 5:+25630213 MsG0580025870.01.T01:CDS
TTCAAGATGCGTTTAACCGT+TGG 0.519238 5:+25630109 MsG0580025870.01.T01:CDS
GGGACATTCTTCTGTTGAGG+AGG 0.520468 5:+25630383 MsG0580025870.01.T01:CDS
ATCAAGTTGGTCATTTACTA+GGG 0.524382 5:+25630358 MsG0580025870.01.T01:CDS
GTTATGCGTTTAAGATAGAA+GGG 0.525080 5:-25629435 None:intergenic
GAAGTTGTTCTTCATGGTGG+TGG 0.525941 5:-25629977 None:intergenic
TGGTGCAAAAGAAATGGATT+CGG 0.529226 5:-25629382 None:intergenic
AATTTCAATCTCAATACTAC+AGG 0.532944 5:+25629663 MsG0580025870.01.T01:CDS
ATAAACAATAGTGTTTATGT+TGG 0.547130 5:+25630243 MsG0580025870.01.T01:CDS
GTCCATTTAAACCGTGGTGG+AGG 0.548682 5:+25630013 MsG0580025870.01.T01:CDS
GGTGTTCCCGACATAATCAA+TGG 0.556418 5:+25629952 MsG0580025870.01.T01:CDS
GGTGGTTCCATTGATTATGT+CGG 0.558548 5:-25629959 None:intergenic
ACGCCGTTGCATTTGACCAA+CGG 0.560186 5:-25630125 None:intergenic
GCAAGAAAAGAAGATTGAGT+TGG 0.560872 5:+25630428 MsG0580025870.01.T01:CDS
TTAACGTTGGATGGAAAAGG+TGG 0.570801 5:+25630201 MsG0580025870.01.T01:CDS
TTCTCCACATTCCTCCACCA+CGG 0.583327 5:-25630024 None:intergenic
TGTTGTTGAAGAGGATGATG+TGG 0.588380 5:+25630314 MsG0580025870.01.T01:CDS
GAGATTGGAGACATCGTTAG+CGG 0.595187 5:+25629519 MsG0580025870.01.T01:CDS
TAGTGAAAATGTTGTTGAAG+AGG 0.596863 5:+25630305 MsG0580025870.01.T01:CDS
TAACCGTTGGTCAAATGCAA+CGG 0.603699 5:+25630122 MsG0580025870.01.T01:CDS
TAAACCGTGGTGGAGGAATG+TGG 0.614425 5:+25630020 MsG0580025870.01.T01:CDS
ATTATTTCAAAGCCACGTTG+TGG 0.619324 5:+25629931 MsG0580025870.01.T01:CDS
TGAATGATGATGATTTGCAA+AGG 0.623412 5:+25630451 MsG0580025870.01.T01:CDS
GGTGAAGTTGTTCTTCATGG+TGG 0.629170 5:-25629980 None:intergenic
TCTGTTGTGATGCATCAAGT+TGG 0.649877 5:+25630345 MsG0580025870.01.T01:CDS
CAAGTCCATTTAAACCGTGG+TGG 0.655517 5:+25630010 MsG0580025870.01.T01:CDS
CTTGGATGCAGATGAACAGT+GGG 0.669119 5:+25630275 MsG0580025870.01.T01:CDS
GTTGGATGGAAAAGGTGGAA+CGG 0.688325 5:+25630206 MsG0580025870.01.T01:CDS
ACTTGGATGCAGATGAACAG+TGG 0.694860 5:+25630274 MsG0580025870.01.T01:CDS
AAACAAGTCCATTTAAACCG+TGG 0.711128 5:+25630007 MsG0580025870.01.T01:CDS
ATGTCGGGAACACCACAACG+TGG 0.747885 5:-25629943 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGAATATTTACAATTCTT+CGG + Chr5:25629555-25629574 MsG0580025870.01.T01:CDS 15.0%
!! TTGTAAATATTCTTTCAAAT+CGG - Chr5:25629550-25629569 None:intergenic 15.0%
!! ATAAACAATAGTGTTTATGT+TGG + Chr5:25630243-25630262 MsG0580025870.01.T01:CDS 20.0%
!! TAATACTAAGAGAAACATAT+AGG - Chr5:25629340-25629359 None:intergenic 20.0%
!!! CGTATCTAAATGATTTTTTT+TGG - Chr5:25629716-25629735 None:intergenic 20.0%
! AATTTCAATCTCAATACTAC+AGG + Chr5:25629663-25629682 MsG0580025870.01.T01:CDS 25.0%
! GATAAATACTATATAGTCAC+TGG + Chr5:25629689-25629708 MsG0580025870.01.T01:intron 25.0%
!!! TTTAAATGGACTTGTTTTGT+TGG - Chr5:25630004-25630023 None:intergenic 25.0%
AATGCAAAGTTGAAGAGTTT+AGG - Chr5:25629584-25629603 None:intergenic 30.0%
CAAAGGATTCAGGAAATTTA+TGG + Chr5:25630468-25630487 MsG0580025870.01.T01:CDS 30.0%
CTAACTATTAATCCAATTGC+TGG - Chr5:25629405-25629424 None:intergenic 30.0%
TAGTGAAAATGTTGTTGAAG+AGG + Chr5:25630305-25630324 MsG0580025870.01.T01:CDS 30.0%
! AGTTATGCGTTTAAGATAGA+AGG - Chr5:25629439-25629458 None:intergenic 30.0%
! GTAAATTATTTTCCAGCATC+TGG + Chr5:25629463-25629482 MsG0580025870.01.T01:CDS 30.0%
! GTTATGCGTTTAAGATAGAA+GGG - Chr5:25629438-25629457 None:intergenic 30.0%
! TCTTCAACAACATTTTCACT+AGG - Chr5:25630306-25630325 None:intergenic 30.0%
! TGAATGATGATGATTTGCAA+AGG + Chr5:25630451-25630470 MsG0580025870.01.T01:CDS 30.0%
! TTTTTAACGTTGGATGGAAA+AGG + Chr5:25630198-25630217 MsG0580025870.01.T01:CDS 30.0%
!! ATCAAGTTGGTCATTTACTA+GGG + Chr5:25630358-25630377 MsG0580025870.01.T01:CDS 30.0%
AAACAAGTCCATTTAAACCG+TGG + Chr5:25630007-25630026 MsG0580025870.01.T01:CDS 35.0%
AATTGCTGGTGCAAAAGAAA+TGG - Chr5:25629391-25629410 None:intergenic 35.0%
ATTATTTCAAAGCCACGTTG+TGG + Chr5:25629931-25629950 MsG0580025870.01.T01:CDS 35.0%
GCAAGAAAAGAAGATTGAGT+TGG + Chr5:25630428-25630447 MsG0580025870.01.T01:CDS 35.0%
TCGTTTGTGACATTATTCTC+TGG - Chr5:25630075-25630094 None:intergenic 35.0%
TTATGTTGGCAGTGTATACT+TGG + Chr5:25630257-25630276 MsG0580025870.01.T01:CDS 35.0%
! ATAGCGTTTTTAACGTTGGA+TGG + Chr5:25630192-25630211 MsG0580025870.01.T01:CDS 35.0%
! ATTTCTTTTGCACCAGCAAT+TGG + Chr5:25629390-25629409 MsG0580025870.01.T01:CDS 35.0%
! TCGTATAGCGTTTTTAACGT+TGG + Chr5:25630188-25630207 MsG0580025870.01.T01:CDS 35.0%
! TGATGATTTGCAAAGGATTC+AGG + Chr5:25630458-25630477 MsG0580025870.01.T01:CDS 35.0%
! TGGTGCAAAAGAAATGGATT+CGG - Chr5:25629385-25629404 None:intergenic 35.0%
! TTCTTTTCTTGCAACGCAAT+CGG - Chr5:25630420-25630439 None:intergenic 35.0%
!! CATCAAGTTGGTCATTTACT+AGG + Chr5:25630357-25630376 MsG0580025870.01.T01:CDS 35.0%
!! TTCAACAAAGCTCTAGAGAT+TGG + Chr5:25629504-25629523 MsG0580025870.01.T01:CDS 35.0%
AGTTGGTCATTTACTAGGGT+TGG + Chr5:25630362-25630381 MsG0580025870.01.T01:CDS 40.0%
CTTGAGTTTATTCCAGATGC+TGG - Chr5:25629478-25629497 None:intergenic 40.0%
GTTGGTCATTTACTAGGGTT+GGG + Chr5:25630363-25630382 MsG0580025870.01.T01:CDS 40.0%
TAACCGTTGGTCAAATGCAA+CGG + Chr5:25630122-25630141 MsG0580025870.01.T01:CDS 40.0%
TCTGTTGTGATGCATCAAGT+TGG + Chr5:25630345-25630364 MsG0580025870.01.T01:CDS 40.0%
TGAAGAGGATGATGTGGATT+TGG + Chr5:25630320-25630339 MsG0580025870.01.T01:CDS 40.0%
TGTTGTTGAAGAGGATGATG+TGG + Chr5:25630314-25630333 MsG0580025870.01.T01:CDS 40.0%
TTAACGTTGGATGGAAAAGG+TGG + Chr5:25630201-25630220 MsG0580025870.01.T01:CDS 40.0%
TTCAAGATGCGTTTAACCGT+TGG + Chr5:25630109-25630128 MsG0580025870.01.T01:CDS 40.0%
! CCAAACAAGTTTTTCTCCAC+AGG + Chr5:25629883-25629902 MsG0580025870.01.T01:intron 40.0%
! CCTGTGGAGAAAAACTTGTT+TGG - Chr5:25629886-25629905 None:intergenic 40.0%
! GGTGGTTCCATTGATTATGT+CGG - Chr5:25629962-25629981 None:intergenic 40.0%
! GTGGTTCCATTGATTATGTC+GGG - Chr5:25629961-25629980 None:intergenic 40.0%
! GTTGGTGAAGTTGTTCTTCA+TGG - Chr5:25629986-25630005 None:intergenic 40.0%
ACTTGGATGCAGATGAACAG+TGG + Chr5:25630274-25630293 MsG0580025870.01.T01:CDS 45.0%
CAAGTCCATTTAAACCGTGG+TGG + Chr5:25630010-25630029 MsG0580025870.01.T01:CDS 45.0%
CTTGGATGCAGATGAACAGT+GGG + Chr5:25630275-25630294 MsG0580025870.01.T01:CDS 45.0%
GAAGTTGTTCTTCATGGTGG+TGG - Chr5:25629980-25629999 None:intergenic 45.0%
GAGATTGGAGACATCGTTAG+CGG + Chr5:25629519-25629538 MsG0580025870.01.T01:CDS 45.0%
GGTGAAGTTGTTCTTCATGG+TGG - Chr5:25629983-25630002 None:intergenic 45.0%
GGTGTTCCCGACATAATCAA+TGG + Chr5:25629952-25629971 MsG0580025870.01.T01:CDS 45.0%
GTTGGATGGAAAAGGTGGAA+CGG + Chr5:25630206-25630225 MsG0580025870.01.T01:CDS 45.0%
GTTGGGACATTCTTCTGTTG+AGG + Chr5:25630380-25630399 MsG0580025870.01.T01:CDS 45.0%
TTCCTCCACCACGGTTTAAA+TGG - Chr5:25630018-25630037 None:intergenic 45.0%
!! ATCTTGATCGAGTTGACCTG+TGG - Chr5:25629902-25629921 None:intergenic 45.0%
ACGCCGTTGCATTTGACCAA+CGG - Chr5:25630128-25630147 None:intergenic 50.0%
GATGGAAAAGGTGGAACGGT+TGG + Chr5:25630210-25630229 MsG0580025870.01.T01:CDS 50.0%
GGGACATTCTTCTGTTGAGG+AGG + Chr5:25630383-25630402 MsG0580025870.01.T01:CDS 50.0%
GTCCATTTAAACCGTGGTGG+AGG + Chr5:25630013-25630032 MsG0580025870.01.T01:CDS 50.0%
TTCTCCACATTCCTCCACCA+CGG - Chr5:25630027-25630046 None:intergenic 50.0%
! TAAACCGTGGTGGAGGAATG+TGG + Chr5:25630020-25630039 MsG0580025870.01.T01:CDS 50.0%
ATGTCGGGAACACCACAACG+TGG - Chr5:25629946-25629965 None:intergenic 55.0%
GGAAAAGGTGGAACGGTTGG+AGG + Chr5:25630213-25630232 MsG0580025870.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr5 gene 25629315 25630503 25629315 ID=MsG0580025870.01;Name=MsG0580025870.01
Chr5 mRNA 25629315 25630503 25629315 ID=MsG0580025870.01.T01;Parent=MsG0580025870.01;Name=MsG0580025870.01.T01;_AED=0.45;_eAED=0.45;_QI=0|0|0|1|1|1|2|0|322
Chr5 exon 25629315 25629684 25629315 ID=MsG0580025870.01.T01:exon:4191;Parent=MsG0580025870.01.T01
Chr5 exon 25629905 25630503 25629905 ID=MsG0580025870.01.T01:exon:4192;Parent=MsG0580025870.01.T01
Chr5 CDS 25629315 25629684 25629315 ID=MsG0580025870.01.T01:cds;Parent=MsG0580025870.01.T01
Chr5 CDS 25629905 25630503 25629905 ID=MsG0580025870.01.T01:cds;Parent=MsG0580025870.01.T01
Gene Sequence

>MsG0580025870.01.T01

ATGAAATTGAAAACACAAAAAACCTATATGTTTCTCTTAGTATTATCTTTTCTACTTGCAGATGTATCCGAATCCATTTCTTTTGCACCAGCAATTGGATTAATAGTTAGAAGCAGAATTCCCTTCTATCTTAAACGCATAACTTCAAGTAAATTATTTTCCAGCATCTGGAATAAACTCAAGTCAATCTTCAACAAAGCTCTAGAGATTGGAGACATCGTTAGCGGAATTTCCGATTTGAAAGAATATTTACAATTCTTCGGTTACCTAAACTCTTCAACTTTGCATTCAAATTTCACAGATGCTTTCACAGAAAATCTTCAATCTGCAATAATCGAATTTCAAACAAATTTCAATCTCAATACTACAGGTCAACTCGATCAAGATATATATGAAATTATTTCAAAGCCACGTTGTGGTGTTCCCGACATAATCAATGGAACCACCACCATGAAGAACAACTTCACCAACAAAACAAGTCCATTTAAACCGTGGTGGAGGAATGTGGAGAACAGATCGTTAGCATATGCTTTTCATCCAGAGAATAATGTCACAAACGATGTTAATTCTTTGTTTCAAGATGCGTTTAACCGTTGGTCAAATGCAACGGCGTTGAACTTCATTGAGACAACGTCGTTTAATGATTCAGATATTCGTATAGCGTTTTTAACGTTGGATGGAAAAGGTGGAACGGTTGGAGGCAGTTATATAAACAATAGTGTTTATGTTGGCAGTGTATACTTGGATGCAGATGAACAGTGGGTGTTACCTAGTGAAAATGTTGTTGAAGAGGATGATGTGGATTTGGAATCTGTTGTGATGCATCAAGTTGGTCATTTACTAGGGTTGGGACATTCTTCTGTTGAGGAGGCTATTATGTATCCGATTGCGTTGCAAGAAAAGAAGATTGAGTTGGTGAATGATGATGATTTGCAAAGGATTCAGGAAATTTATGGTGTCAATACTTGA

Protein sequence

>MsG0580025870.01.T01

MKLKTQKTYMFLLVLSFLLADVSESISFAPAIGLIVRSRIPFYLKRITSSKLFSSIWNKLKSIFNKALEIGDIVSGISDLKEYLQFFGYLNSSTLHSNFTDAFTENLQSAIIEFQTNFNLNTTGQLDQDIYEIISKPRCGVPDIINGTTTMKNNFTNKTSPFKPWWRNVENRSLAYAFHPENNVTNDVNSLFQDAFNRWSNATALNFIETTSFNDSDIRIAFLTLDGKGGTVGGSYINNSVYVGSVYLDADEQWVLPSENVVEEDDVDLESVVMHQVGHLLGLGHSSVEEAIMYPIALQEKKIELVNDDDLQRIQEIYGVNT*