AlfalfaGEDB | MsG0380013531.01

M. sativa cultivar ZhongmuNo.1 / MsG0380013531.01

KEGG: -
GO: GO:0008152 GO:0016787
PFAM: PF07859

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013531.01.T01	XP_013459645.1	84.848	132	15	1	5	136	207	333	2.95E-74	234

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013531.01.T01	Q5NUF4	50.82	122	60	0	13	134	206	327	1.35E-37	133

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013531.01.T01	A0A072UW70	84.848	132	15	1	5	136	207	333	1.41e-74	234

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048748.01	MsG0380013531.01	0.802950	4.399565e-49	2.183339e-46
MsG0080048938.01	MsG0380013531.01	0.823400	1.413963e-53	1.216022e-50
MsG0180002184.01	MsG0380013531.01	0.809438	1.892014e-50	1.110870e-47
MsG0180003189.01	MsG0380013531.01	0.802470	5.524932e-49	2.708605e-46
MsG0180004025.01	MsG0380013531.01	0.804233	2.383796e-49	1.222556e-46
MsG0180005005.01	MsG0380013531.01	0.847069	1.459656e-59	2.564106e-56
MsG0180005339.01	MsG0380013531.01	0.805488	1.302816e-49	6.901545e-47
MsG0380013531.01	MsG0380015847.01	-0.820859	5.488832e-53	4.394382e-50
MsG0380013531.01	MsG0480018426.01	0.807284	5.448054e-50	3.022477e-47
MsG0380013531.01	MsG0480019384.01	0.805868	1.084192e-49	5.799944e-47
MsG0380013531.01	MsG0480020322.01	0.820372	7.102159e-53	5.609507e-50
MsG0380013531.01	MsG0480020323.01	0.812190	4.802980e-51	3.033530e-48
MsG0380013531.01	MsG0580024775.01	0.825777	3.896244e-54	3.585985e-51
MsG0380013531.01	MsG0580025280.01	0.820072	8.318733e-53	6.516415e-50
MsG0380013531.01	MsG0580029237.01	0.814219	1.721988e-51	1.148569e-48
MsG0380013531.01	MsG0680031334.01	0.806471	8.095783e-50	4.397929e-47
MsG0380013531.01	MsG0780036803.01	0.806397	8.390919e-50	4.549457e-47
MsG0380013531.01	MsG0780040177.01	0.805851	1.093142e-49	5.845398e-47
MsG0380013531.01	MsG0780041747.01	0.802064	6.697083e-49	3.249465e-46
MsG0380013531.01	MsG0880041852.01	0.804611	1.987999e-49	1.029595e-46
MsG0380013531.01	MsG0880043829.01	0.824378	8.338794e-54	7.373302e-51
MsG0380013531.01	MsG0880045285.01	0.811465	6.906897e-51	4.278499e-48
MsG0380013531.01	MsG0880045783.01	0.803148	4.003177e-49	1.996734e-46
MsG0380013531.01	MsG0880046542.01	0.812685	3.744368e-51	2.396306e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013531.01.T01	MTR_3g450680	84.848	132	15	1	5	136	207	333	3.57e-78	234
MsG0380013531.01.T01	MTR_8g040860	81.301	123	23	0	14	136	208	330	1.04e-69	213
MsG0380013531.01.T01	MTR_7g051020	55.970	134	57	1	5	136	194	327	2.27e-49	160
MsG0380013531.01.T01	MTR_1g104930	62.400	125	45	2	14	136	200	324	4.81e-43	144
MsG0380013531.01.T01	MTR_5g005920	51.515	132	62	1	7	136	201	332	1.61e-42	143
MsG0380013531.01.T01	MTR_1g104933	59.677	124	48	1	13	136	197	318	1.99e-42	142
MsG0380013531.01.T01	MTR_5g005850	56.034	116	51	0	19	134	213	328	2.42e-42	142
MsG0380013531.01.T01	MTR_1g105020	47.541	122	64	0	13	134	201	322	5.89e-35	123
MsG0380013531.01.T01	MTR_5g005900	47.727	132	67	1	7	136	197	328	3.07e-32	116
MsG0380013531.01.T01	MTR_4g086480	41.085	129	73	2	7	135	194	319	1.46e-27	103
MsG0380013531.01.T01	MTR_4g086470	44.444	117	62	2	20	136	206	319	4.14e-23	92.4
MsG0380013531.01.T01	MTR_3g101420	38.983	118	71	1	19	136	200	316	3.73e-22	89.7
MsG0380013531.01.T01	MTR_4g086510	34.400	125	78	3	12	135	197	318	2.86e-19	82.0
MsG0380013531.01.T01	MTR_4g086540	48.649	74	38	0	61	134	231	304	1.45e-18	80.1
MsG0380013531.01.T01	MTR_7g056207	35.043	117	70	4	20	134	237	349	5.68e-17	75.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013531.01.T01	AT1G47480	37.288	118	73	1	19	136	195	311	4.99e-23	92.0
MsG0380013531.01.T01	AT3G48700	40.594	101	59	1	36	135	226	326	1.40e-19	82.8
MsG0380013531.01.T01	AT2G03550	38.000	100	59	2	37	136	216	312	3.19e-18	79.0
MsG0380013531.01.T01	AT1G19190	34.615	104	65	1	33	136	215	315	5.50e-18	78.6
MsG0380013531.01.T01	AT1G49650	39.216	102	60	2	35	135	273	373	4.61e-14	67.8
MsG0380013531.01.T01	AT3G48690	35.354	99	64	0	37	135	223	321	7.44e-14	67.0
MsG0380013531.01.T01	AT1G49640	31.405	121	75	3	21	134	194	313	1.01e-11	61.2

Find 36 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACCTTATGATGTTAAATATT+AGG	0.247847	3:+40010258	None:intergenic
AGAATTATTATTTGTGTTGC+TGG	0.269306	3:+40010470	MsG0380013531.01.T01:CDS
ATCTTGCCACTCTCAGATTC+TGG	0.270525	3:-40010614	None:intergenic
TAACCCTTTGATTAATCCTT+TGG	0.292699	3:+40010409	MsG0380013531.01.T01:CDS
TCAATTCCAAAAGGTGCATT+TGG	0.294236	3:-40010386	None:intergenic
TTTCCATAAGGTTTGGCATT+TGG	0.301055	3:+40010358	MsG0380013531.01.T01:CDS
TTTCAAAGCTCTACCCTATT+GGG	0.301225	3:+40010303	MsG0380013531.01.T01:CDS
ACACCAAATGCCAAACCTTA+TGG	0.304815	3:-40010361	None:intergenic
GGGACAGAGGAGTTTGGTAT+TGG	0.336444	3:+40010507	MsG0380013531.01.T01:CDS
TAGTGGGTGGAATGGGAAAT+TGG	0.342499	3:+40010544	MsG0380013531.01.T01:CDS
AACCCTTTGATTAATCCTTT+GGG	0.363602	3:+40010410	MsG0380013531.01.T01:CDS
GTGTACCCAAATGCACCTTT+TGG	0.365720	3:+40010380	MsG0380013531.01.T01:CDS
AGGGTAGAGCTTTGAAAATA+AGG	0.367189	3:-40010297	None:intergenic
ATAATAGGTTCTGACCCAAT+AGG	0.376755	3:-40010317	None:intergenic
GGACAGAGGAGTTTGGTATT+GGG	0.400599	3:+40010508	MsG0380013531.01.T01:CDS
AGTTAAGGGACAGAGGAGTT+TGG	0.407607	3:+40010501	MsG0380013531.01.T01:CDS
CAATTCCAAAAGGTGCATTT+GGG	0.417461	3:-40010385	None:intergenic
TCACGACCCTTCTTCACTCT+TGG	0.420561	3:-40010664	None:intergenic
TTGCTGGAAAAGATAAGTTA+AGG	0.423689	3:+40010486	MsG0380013531.01.T01:CDS
CTCCCAAAGGATTAATCAAA+GGG	0.429238	3:-40010412	None:intergenic
TAATAGGTTCTGACCCAATA+GGG	0.452643	3:-40010316	None:intergenic
TGGGAATGTGTGAAGAATAG+TGG	0.473385	3:+40010527	MsG0380013531.01.T01:CDS
TGCTGGAAAAGATAAGTTAA+GGG	0.485690	3:+40010487	MsG0380013531.01.T01:CDS
TTGATTAATCCTTTGGGAGA+AGG	0.485999	3:+40010416	MsG0380013531.01.T01:CDS
TGAAGAATAGTGGGTGGAAT+GGG	0.506837	3:+40010537	MsG0380013531.01.T01:CDS
CAAGATATTGATCCAACGAT+TGG	0.511817	3:+40010631	MsG0380013531.01.T01:CDS
TCTCCCAAAGGATTAATCAA+AGG	0.514419	3:-40010413	None:intergenic
AAAGATAAGTTAAGGGACAG+AGG	0.546448	3:+40010494	MsG0380013531.01.T01:CDS
AAAGGGTTATCAATTCCAAA+AGG	0.547123	3:-40010395	None:intergenic
GGACAAAACTAACCAATCGT+TGG	0.553376	3:-40010643	None:intergenic
GGGAATGTGTGAAGAATAGT+GGG	0.579984	3:+40010528	MsG0380013531.01.T01:CDS
CTAGGTGCACCTTCTCCCAA+AGG	0.593677	3:-40010425	None:intergenic
GTGAAGAATAGTGGGTGGAA+TGG	0.599012	3:+40010536	MsG0380013531.01.T01:CDS
GTTTAAGCAAATGATAAACA+TGG	0.602550	3:-40010592	None:intergenic
CTTAAACCAGAATCTGAGAG+TGG	0.624713	3:+40010608	MsG0380013531.01.T01:CDS
AATGTGTGAAGAATAGTGGG+TGG	0.742181	3:+40010531	MsG0380013531.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TGAAAATAATCTTTTCCATA+AGG	+	Chr3:40010346-40010365	MsG0380013531.01.T01:CDS	20.0%
!!!	TTATGGAAAAGATTATTTTC+AGG	-	Chr3:40010347-40010366	None:intergenic	20.0%
!	AGAATTATTATTTGTGTTGC+TGG	+	Chr3:40010470-40010489	MsG0380013531.01.T01:CDS	25.0%
!	GTTTAAGCAAATGATAAACA+TGG	-	Chr3:40010595-40010614	None:intergenic	25.0%
!!	ATAATCTTTTCCATAAGGTT+TGG	+	Chr3:40010351-40010370	MsG0380013531.01.T01:CDS	25.0%
!!!	TTATTTTCAGGCTCAATAAT+AGG	-	Chr3:40010335-40010354	None:intergenic	25.0%
	AAAGGGTTATCAATTCCAAA+AGG	-	Chr3:40010398-40010417	None:intergenic	30.0%
	AACCCTTTGATTAATCCTTT+GGG	+	Chr3:40010410-40010429	MsG0380013531.01.T01:CDS	30.0%
	TAACCCTTTGATTAATCCTT+TGG	+	Chr3:40010409-40010428	MsG0380013531.01.T01:CDS	30.0%
	TGCTGGAAAAGATAAGTTAA+GGG	+	Chr3:40010487-40010506	MsG0380013531.01.T01:CDS	30.0%
	TTGCTGGAAAAGATAAGTTA+AGG	+	Chr3:40010486-40010505	MsG0380013531.01.T01:CDS	30.0%
	AAAGATAAGTTAAGGGACAG+AGG	+	Chr3:40010494-40010513	MsG0380013531.01.T01:CDS	35.0%
	CAAGATATTGATCCAACGAT+TGG	+	Chr3:40010631-40010650	MsG0380013531.01.T01:CDS	35.0%
	CTCCCAAAGGATTAATCAAA+GGG	-	Chr3:40010415-40010434	None:intergenic	35.0%
	TCTCCCAAAGGATTAATCAA+AGG	-	Chr3:40010416-40010435	None:intergenic	35.0%
	TTTCAAAGCTCTACCCTATT+GGG	+	Chr3:40010303-40010322	MsG0380013531.01.T01:CDS	35.0%
	TTTCCATAAGGTTTGGCATT+TGG	+	Chr3:40010358-40010377	MsG0380013531.01.T01:CDS	35.0%
!	ATAATAGGTTCTGACCCAAT+AGG	-	Chr3:40010320-40010339	None:intergenic	35.0%
!	CAATTCCAAAAGGTGCATTT+GGG	-	Chr3:40010388-40010407	None:intergenic	35.0%
!	TAATAGGTTCTGACCCAATA+GGG	-	Chr3:40010319-40010338	None:intergenic	35.0%
!	TCAATTCCAAAAGGTGCATT+TGG	-	Chr3:40010389-40010408	None:intergenic	35.0%
!	TTGATTAATCCTTTGGGAGA+AGG	+	Chr3:40010416-40010435	MsG0380013531.01.T01:CDS	35.0%
!	TTTTCAAAGCTCTACCCTAT+TGG	+	Chr3:40010302-40010321	MsG0380013531.01.T01:CDS	35.0%
!!	AGGGTAGAGCTTTGAAAATA+AGG	-	Chr3:40010300-40010319	None:intergenic	35.0%
!!!	CAACCAAGTTTTTTCAAGCT+AGG	-	Chr3:40010446-40010465	None:intergenic	35.0%
	ACACCAAATGCCAAACCTTA+TGG	-	Chr3:40010364-40010383	None:intergenic	40.0%
	CTTAAACCAGAATCTGAGAG+TGG	+	Chr3:40010608-40010627	MsG0380013531.01.T01:CDS	40.0%
	GCACCTAGCTTGAAAAAACT+TGG	+	Chr3:40010440-40010459	MsG0380013531.01.T01:CDS	40.0%
	GGACAAAACTAACCAATCGT+TGG	-	Chr3:40010646-40010665	None:intergenic	40.0%
	GGGAATGTGTGAAGAATAGT+GGG	+	Chr3:40010528-40010547	MsG0380013531.01.T01:CDS	40.0%
	TGGGAATGTGTGAAGAATAG+TGG	+	Chr3:40010527-40010546	MsG0380013531.01.T01:CDS	40.0%
!	AATGTGTGAAGAATAGTGGG+TGG	+	Chr3:40010531-40010550	MsG0380013531.01.T01:CDS	40.0%
!!	TGAAGAATAGTGGGTGGAAT+GGG	+	Chr3:40010537-40010556	MsG0380013531.01.T01:CDS	40.0%
	AGTTAAGGGACAGAGGAGTT+TGG	+	Chr3:40010501-40010520	MsG0380013531.01.T01:CDS	45.0%
	ATCTTGCCACTCTCAGATTC+TGG	-	Chr3:40010617-40010636	None:intergenic	45.0%
	GGACAGAGGAGTTTGGTATT+GGG	+	Chr3:40010508-40010527	MsG0380013531.01.T01:CDS	45.0%
!	GTGTACCCAAATGCACCTTT+TGG	+	Chr3:40010380-40010399	MsG0380013531.01.T01:CDS	45.0%
!	TAGTGGGTGGAATGGGAAAT+TGG	+	Chr3:40010544-40010563	MsG0380013531.01.T01:CDS	45.0%
!!	GTGAAGAATAGTGGGTGGAA+TGG	+	Chr3:40010536-40010555	MsG0380013531.01.T01:CDS	45.0%
	GGGACAGAGGAGTTTGGTAT+TGG	+	Chr3:40010507-40010526	MsG0380013531.01.T01:CDS	50.0%
	CTAGGTGCACCTTCTCCCAA+AGG	-	Chr3:40010428-40010447	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	40010263	40010673	40010263	ID=MsG0380013531.01;Name=MsG0380013531.01
Chr3	mRNA	40010263	40010673	40010263	ID=MsG0380013531.01.T01;Parent=MsG0380013531.01;Name=MsG0380013531.01.T01;_AED=0.34;_eAED=0.34;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|136
Chr3	exon	40010263	40010673	40010263	ID=MsG0380013531.01.T01:exon:22357;Parent=MsG0380013531.01.T01
Chr3	CDS	40010263	40010673	40010263	ID=MsG0380013531.01.T01:cds;Parent=MsG0380013531.01.T01

Gene Sequence

>MsG0380013531.01.T01

ATGATGTTAAATATTAGGAGCTATTTTAATTCATCCTTATTTTCAAAGCTCTACCCTATTGGGTCAGAACCTATTATTGAGCCTGAAAATAATCTTTTCCATAAGGTTTGGCATTTGGTGTACCCAAATGCACCTTTTGGAATTGATAACCCTTTGATTAATCCTTTGGGAGAAGGTGCACCTAGCTTGAAAAAACTTGGTTGTTCTAGAATTATTATTTGTGTTGCTGGAAAAGATAAGTTAAGGGACAGAGGAGTTTGGTATTGGGAATGTGTGAAGAATAGTGGGTGGAATGGGAAATTGGAATTCTTTGAAGAGAAAGATGAGAACCATGTTTATCATTTGCTTAAACCAGAATCTGAGAGTGGCAAGATATTGATCCAACGATTGGTTAGTTTTGTCCAAGAGTGA

Protein sequence

>MsG0380013531.01.T01

MMLNIRSYFNSSLFSKLYPIGSEPIIEPENNLFHKVWHLVYPNAPFGIDNPLINPLGEGAPSLKKLGCSRIIICVAGKDKLRDRGVWYWECVKNSGWNGKLEFFEEKDENHVYHLLKPESESGKILIQRLVSFVQE*