AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780036803.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036803.01.T01 MTR_7g019160 98.387 62 1 0 1 62 1 62 1.89e-37 119
MsG0780036803.01.T01 MTR_6g016890 68.333 60 19 0 1 60 2 61 1.69e-22 81.3
MsG0780036803.01.T01 MTR_5g005330 64.516 62 16 3 3 58 5 66 2.71e-15 63.5
MsG0780036803.01.T02 MTR_7g019160 97.297 37 1 0 1 37 26 62 2.39e-19 72.4
MsG0780036803.01.T02 MTR_6g016890 80.000 35 7 0 1 35 27 61 7.27e-14 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 8 sgRNAs with CRISPR-Local

Find 7 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTCTCTTCAATAAGCTTTC+TGG 0.211273 7:-14192574 None:intergenic
CAATTTCAAAGACTTTATTT+TGG 0.218407 7:-14192487 None:intergenic
GATTATGGGTCTTGGAAGAT+TGG 0.341904 7:+14192449 None:intergenic
CTCTTCTCCATCTTATCATA+TGG 0.344037 7:-14192516 None:intergenic
AAGAAAGGGATTATGGGTCT+TGG 0.427535 7:+14192441 None:intergenic
GAAGAAGCCATATGATAAGA+TGG 0.518528 7:+14192509 MsG0780036803.01.T01:CDS
ATGGGTCTTGGAAGATTGGT+AGG 0.523383 7:+14192453 MsG0780036803.01.T01:CDS
GAAGAGTGAGAGTATGAGAG+TGG 0.529189 7:+14192533 MsG0780036803.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAATTTCAAAGACTTTATTT+TGG - Chr7:14192490-14192509 None:intergenic 20.0%
!!! AAGTTGAAAGTTTTAGTCTT+TGG - Chr7:14192618-14192637 None:intergenic 25.0%
AGTCTCTTCAATAAGCTTTC+TGG - Chr7:14192577-14192596 None:intergenic 35.0%
CTCTTCTCCATCTTATCATA+TGG - Chr7:14192519-14192538 None:intergenic 35.0%
GAAGAAGCCATATGATAAGA+TGG + Chr7:14192509-14192528 MsG0780036803.01.T01:CDS 35.0%
ATGGGTCTTGGAAGATTGGT+AGG + Chr7:14192453-14192472 MsG0780036803.01.T01:CDS 45.0%
! GAAGAGTGAGAGTATGAGAG+TGG + Chr7:14192533-14192552 MsG0780036803.01.T01:CDS 45.0%
Chromosome Type Strat End Strand Name
Chr7 gene 14192453 14192641 14192453 ID=MsG0780036803.01;Name=MsG0780036803.01
Chr7 mRNA 14192453 14192641 14192453 ID=MsG0780036803.01.T01;Parent=MsG0780036803.01;Name=MsG0780036803.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|62
Chr7 exon 14192453 14192641 14192453 ID=MsG0780036803.01.T01:exon:2925;Parent=MsG0780036803.01.T01
Chr7 CDS 14192453 14192641 14192453 ID=MsG0780036803.01.T01:cds;Parent=MsG0780036803.01.T01
Chr7 mRNA 14192528 14192641 14192528 ID=MsG0780036803.01.T02;Parent=MsG0780036803.01;Name=MsG0780036803.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|37
Chr7 exon 14192528 14192641 14192528 ID=MsG0780036803.01.T02:exon:2926;Parent=MsG0780036803.01.T02
Chr7 CDS 14192528 14192641 14192528 ID=MsG0780036803.01.T02:cds;Parent=MsG0780036803.01.T02
Gene Sequence

>MsG0780036803.01.T01

ATGGGTCTTGGAAGATTGGTAGGAACACTAAAATCCAAAATAAAGTCTTTGAAATTGAAGAAGCCATATGATAAGATGGAGAAGAGTGAGAGTATGAGAGTGGAGATAAAGAGCAGAAAAGCCAGAAAGCTTATTGAAGAGACTCTCAAAATTGCTGATTCACCAAAGACTAAAACTTTCAACTTCTGA

>MsG0780036803.01.T02

ATGGAGAAGAGTGAGAGTATGAGAGTGGAGATAAAGAGCAGAAAAGCCAGAAAGCTTATTGAAGAGACTCTCAAAATTGCTGATTCACCAAAGACTAAAACTTTCAACTTCTGA

Protein sequence

>MsG0780036803.01.T01

MGLGRLVGTLKSKIKSLKLKKPYDKMEKSESMRVEIKSRKARKLIEETLKIADSPKTKTFNF*

>MsG0780036803.01.T02

MEKSESMRVEIKSRKARKLIEETLKIADSPKTKTFNF*